Rh7CG292700

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0009053
Physical Location & Seq
Reverse (-)
29879322 .. 29883315
3994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG292700.1

Sequence Viewer

Length: 2637 bp
ATGGACGTGAGGCTAACGGAGGGGGCGATACTGAAGATCACGACGGAAGACCATGACGCGCAGTATAGGCCCATCCTGCAAGTGCACGACATGAAGCAGATCCACGCACAGGGCGGCGCCGGCGCGGACAAAGAGCGCTACAGGCTGTCCTTGTCCGATGGGTCCCACACCCAGCAGGGGATGATTGCCACTCAGCAGAACTTCCTGATTCGGCAGGGCCACCTCCAGAAGGGCTCCGTCGTCTGCCTCAATCAATTCACCTGCACCACCGTCCAAGGCCGCCAGATCATCATTATAATCTCATTGGATGTGCTAGTTGATACATGTGAAATCATTGGACAGCCTGCCAGAATTTCGAATGCCCAGTCCCCAGAGACAATACCTGGAAATGCTCTATCTCTTAACAGCAGTGCTCGTGTGGCGGGTCATGAAACTATGACTGAGGCGACTCCCCAACAACCTAGATTGAATCAATCGTATGGTGGTTCTTATTCTGGTAGTGCTGACCCAGGAAGATATGCTACAATGAGTACAGTTCCCAATCACCCTAGACCAGAGACTGGTTCTGGTTTTGCTGGGTTAGCACCAGTAAGCGGGTTATATGCTGGTCAGAACACAGGTTTACGCAGCCCCATATCCCAGGTTCCACCTCCACTTCAAAATTCTTATGCTCAGCCGACTTATCAACAGTCACACTCAAATTCTTATGCCCGCCCGCCTCAGGCAACATATCAACAGCCACCTCCAAGTTCTTATGCACGCCCACCTCAGCCAACTCATCAACAGCCACCTCCATTATATAGTAACAGAGGACCAGTTGCCAAAAATGAAGCTCCTGGTAGAATAGTACAAATTTGTGCCCTGAATCGGTACCTAAATAAGTGGACAATTAAGGCTAGAGTGACGGCTAAATCAGAACTCAGGTCCTACACCAATGCACGTGGTGAGGGGAAAGTATTTAATTTTGATCTCATTGATTGTGGTGGGGAAATTCGTGTGACCTGCTTTAATTCAATAGCTGAAGAATTCTACAATTTGATTGAAGTTGGTAAGGTGTACTTGATTTCTAAGGGTACAGTAAAGGCAGCTGTAAAGAAATTCAACCACCTTAGTAACGAGTTTGAAATTCAATTGGACACCACTTCGACAATACAGTTATGTTTTGAGGATGATGGCTCGATTCCACAGCAGCGGTTCAATTTTCGGCCAATAAGTGATGTTGAAGGTTTGGAGAACAACACTATTGTGGATATAATAGGTGTGGTAACATTCATCAGTCCTGCTGCTTCAATAAGCACGAAAAACGGTGAGACACAAAAGAGAACTCTCCAGTTGAAGGATATGTCTTGTCGTAGCGTTGAAGTAACTCTGTGGGGCAATTTGTGCAATGCAGAAGGGCAAAAACTTCAAAGTATGTGTGATTCTGGGTTATTTCCAGTGCTAGCTGTTAAAGCTGTTAGAGTCAATGACTTCAATGGCAAGGGGGTGGGGACCATGGCTACTAGTCAACTCTTTATAGAGCCCAATAACTCTCAAGCTGATGAAGTGAGGGAATGGTTTGACAGGGAAGGAAGGAATTCCCCATGTGTCTCTATCTCCAGGGGGACAGCAAGTGTGGGGAGAAATGATATCCGAAAGACTATATCCCAGATTAAAGATGAGAAGCTAGGGACTTCTGAGAAGCCAGATTGGATCACAATCAGTGCTACTGTCATATTTGTAAAGAGTGATAGCTACTTCTATGCAGCATGCCCACTCAAGATAGGGGAGCGGCAATGCAACAAAAAGGTTATAAATGATGGAGACGGGACATGGCGGTGTGAAAGATGTGAGCAGTCCGTAGAACAGTGTGACTACAGATATATACTCAACTTACAGATACAAGACCATACTGGCATAGCATGGGTAACTGCATTTCAGGAGGCTGGTGAAGAAATAATGGGTATAACTGCAAAAGATTTTCATAATTTGAAATATGAAATGCAGGATGAAGATAAAGTTGCAGAAATCGTTCGAAGGGTCTTGTTTACTGAATTTGTTTTCAAGTTGAAAATAAAGGAGGAGACTTTTAGTGATGAGCAGCGTGTGAAATCAACAATTGTCAAAGCAGATAAAGTTAGTTTCTCTTCAAAATCTAGATTTCATTTAGATCTGATGGATAGACTTAAAAATGGGGAAACTGCAATACCTTGTGCCGGGATGAACAATACTGAAGTCGGGAGTTCTGCAATTAGACAACATACACCAGCTGTGAATGCTAGTTACGGTACCAATGCTGTCAATGCTGGTAGAGAGTTTGGAGCACCAGCAAATCAAGTACATGTTGGAAGCCAGTATATTAGTCCTAGGGTTCCTTCAACTGGCTCTGCTGGTTTGTATCCTACCTGTAACATCTGTGGAGGTACAAGCCATAATTCTTTGAACTGCCCAAATGTTATGAATGGCCCAGGACAGTCATCAGGAGGGGGTTACACCAATAGTAGGCCAGTTGCCAGTAATTCTGGTGAATGCTATAAATGTCACCAACCTGGGCACTGGGCGAAAGACTGTCCTAGCTCGAACCATATTCCAGCTTATGGAAGGAGTGGAGTCCAGCCGGGCAGATATGGAGTTTCAAATCAAAGGGTCAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

878

Amino Acids

96.27

Weight (kDa)

8.19

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 106 9.3e-26 Replication factor-A protein 1, N-terminal domain
tRNA_anti-codon PF01336 295 - 378 3.1e-10 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 404 - 506 7.4e-26 Replication protein A OB domain
Rep_fac-A_C PF08646 564 - 713 1.2e-49 Replication factor-A C terminal domain
zf-CCHC PF00098 836 - 852 6.6e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 296, 1793
AarI CACCTGC 1 cut(s) 269
Acc36I ACCTGC 2 cut(s) 269, 1010
Acc65I GGTACC 2 cut(s) 870, 2267
AccB1I GGYRCC 3 cut(s) 116, 870, 2267
AccB7I CCANNNNNTGG 2 cut(s) 560, 2576
AccBSI CCGCTC 1 cut(s) 1771
AccII CGCG 2 cut(s) 59, 125
AclWI GGATC 2 cut(s) 94, 1700
AcoI YGGCCR 1 cut(s) 1205
AcuI CTGAAG 3 cut(s) 53, 1041, 2232
AcvI CACGTG 1 cut(s) 941
AcyI GRCGYC 1 cut(s) 117
AdeI CACNNNGTG 1 cut(s) 944
AfaI GTAC 8 cut(s) 532, 849, 872, 1058, 1075, 2269, 2319, 2404
AfeI AGCGCT 1 cut(s) 137
AflIII ACRYGT 2 cut(s) 323, 2320
AhlI ACTAGT 1 cut(s) 1502
AjiI CACGTC 1 cut(s) 7
AjnI CCWGG 7 cut(s) 382, 508, 639, 835, 1598, 2446, 2527
AleI CACNNNNGTG 1 cut(s) 1244
Alw21I GWGCWC 3 cut(s) 87, 415, 2305
Alw26I GTCTC 6 cut(s) 368, 551, 1304, 1594, 1797, 2057
Alw44I GTGCAC 1 cut(s) 83
AlwI GGATC 2 cut(s) 94, 1700
AlwNI CAGNNNCTG 1 cut(s) 560
Aor51HI AGCGCT 1 cut(s) 137
AoxI GGCC 6 cut(s) 68, 217, 277, 1205, 2443, 2483
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 6 cut(s) 627, 1085, 1189, 1283, 1745, 2080
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
Asp700I GAANNNNTTC 2 cut(s) 1576, 2010
Asp718I GGTACC 2 cut(s) 870, 2267
AspA2I CCTAGG 1 cut(s) 2345
AspLEI GCGC 4 cut(s) 61, 119, 125, 138
AspS9I GGNCC 7 cut(s) 69, 162, 217, 812, 924, 1491, 2444
AsuC2I CCSGG 2 cut(s) 2197, 2598
AsuHPI GGTGA 7 cut(s) 250, 536, 956, 1319, 1940, 2513, 2516
AsuII TTCGAA 2 cut(s) 356, 2014
AsuNHI GCTAGC 1 cut(s) 1441
AvaII GGWCC 4 cut(s) 162, 812, 924, 1491
AvrII CCTAGG 1 cut(s) 2345
AxyI CCTNAGG 1 cut(s) 720
BaeGI GKGCMC 3 cut(s) 87, 862, 2535
BanI GGYRCC 3 cut(s) 116, 870, 2267
BanII GRGCYC 2 cut(s) 236, 1524
BarI GAAGNNNNNNTAC 2 cut(s) 505, 537
BauI CACGAG 1 cut(s) 414
BbrPI CACGTG 1 cut(s) 941
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 3 cut(s) 87, 415, 2305
BbvCI CCTCAGC 1 cut(s) 768
BbvI GCAGC 6 cut(s) 639, 1097, 1201, 1270, 1757, 2092
BccI CCATC 5 cut(s) 80, 152, 1166, 1793, 2149
BceAI ACGGC 1 cut(s) 921
BciT130I CCWGG 7 cut(s) 384, 510, 641, 837, 1600, 2448, 2529
BciVI GTATCC 1 cut(s) 2388
BcnI CCSGG 2 cut(s) 2197, 2598
BcoDI GTCTC 6 cut(s) 368, 551, 1304, 1594, 1797, 2057
BcuI ACTAGT 1 cut(s) 1502
BfmI CTRYAG 2 cut(s) 139, 1855
BfoI RGCGCY 2 cut(s) 120, 139
BfuAI ACCTGC 2 cut(s) 269, 1010
BfuI GTATCC 1 cut(s) 2388
BglII AGATCT 1 cut(s) 2149
BisI GCNGC 9 cut(s) 115, 280, 628, 1086, 1190, 1284, 1746, 1772, 2081
BlnI CCTAGG 1 cut(s) 2345
BlpI GCTNAGC 1 cut(s) 672
BlsI GCNGC 9 cut(s) 116, 281, 629, 1087, 1191, 1285, 1747, 1773, 2082
Bme1390I CCNGG 9 cut(s) 384, 510, 641, 837, 1600, 2197, 2448, 2529, 2598
Bme18I GGWCC 4 cut(s) 162, 812, 924, 1491
BmgBI CACGTC 1 cut(s) 7
BmgT120I GGNCC 7 cut(s) 69, 162, 217, 812, 924, 1491, 2444
BmiI GGNNCC 9 cut(s) 118, 163, 164, 235, 645, 872, 1492, 2269, 2352
BmrFI CCNGG 9 cut(s) 384, 510, 641, 837, 1600, 2197, 2448, 2529, 2598
BmrI ACTGGG 2 cut(s) 358, 2545
BmtI GCTAGC 1 cut(s) 1445
BmuI ACTGGG 2 cut(s) 358, 2545
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 3 cut(s) 209, 1313, 1582
Bpu10I CCTNAGC 1 cut(s) 768
Bpu1102I GCTNAGC 1 cut(s) 672
Bpu14I TTCGAA 2 cut(s) 356, 2014
BpuEI CTTGAG 2 cut(s) 1518, 1742
BpuMI CCSGG 2 cut(s) 2197, 2598
BsaAI YACGTR 1 cut(s) 941
BsaBI GATNNNNATC 1 cut(s) 1697
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 8 cut(s) 274, 508, 639, 1494, 1599, 2345, 2446, 2528
Bse118I RCCGGY 1 cut(s) 119
Bse21I CCTNAGG 1 cut(s) 720
Bse3DI GCAATG 2 cut(s) 1393, 1781
Bse8I GATNNNNATC 1 cut(s) 1697
BseBI CCWGG 7 cut(s) 384, 510, 641, 837, 1600, 2448, 2529
BseDI CCNNGG 8 cut(s) 274, 508, 639, 1494, 1599, 2345, 2446, 2528
BseGI GGATG 6 cut(s) 72, 186, 313, 1174, 1993, 2205
BseJI GATNNNNATC 1 cut(s) 1697
BseMI GCAATG 2 cut(s) 1393, 1781
BseMII CTCAG 7 cut(s) 206, 432, 686, 734, 782, 934, 1668
BseRI GAGGAG 1 cut(s) 2075
BseSI GKGCMC 3 cut(s) 87, 862, 2535
BseXI GCAGC 6 cut(s) 639, 1097, 1201, 1270, 1757, 2092
BseYI CCCAGC 2 cut(s) 171, 575
BsgI GTGCAG 1 cut(s) 247
Bsh1236I CGCG 2 cut(s) 59, 125
BshFI GGCC 6 cut(s) 70, 219, 279, 1207, 2445, 2485
BshNI GGYRCC 3 cut(s) 116, 870, 2267
BsiHKAI GWGCWC 3 cut(s) 87, 415, 2305
BsiSI CCGG 3 cut(s) 120, 2196, 2597
BslFI GGGAC 6 cut(s) 148, 352, 1504, 1618, 1684, 1822
BsmAI GTCTC 6 cut(s) 368, 551, 1304, 1594, 1797, 2057
BsmBI CGTCTC 1 cut(s) 1797
BsmFI GGGAC 6 cut(s) 148, 352, 1504, 1618, 1684, 1822
BsmI GAATGC 3 cut(s) 364, 2260, 2513
BsnI GGCC 6 cut(s) 70, 219, 279, 1207, 2445, 2485
Bsp119I TTCGAA 2 cut(s) 356, 2014
Bsp1286I GDGCHC 7 cut(s) 87, 236, 415, 862, 1524, 2305, 2535
Bsp143I GATC 6 cut(s) 36, 99, 285, 967, 1692, 2149
Bsp1720I GCTNAGC 1 cut(s) 672
Bsp19I CCATGG 1 cut(s) 1494
BspANI GGCC 6 cut(s) 70, 219, 279, 1207, 2445, 2485
BspCNI CTCAG 7 cut(s) 205, 433, 685, 733, 781, 933, 1669
BspFNI CGCG 2 cut(s) 59, 125
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 9 cut(s) 118, 163, 164, 235, 645, 872, 1492, 2269, 2352
BspMI ACCTGC 2 cut(s) 269, 1010
BspOI GCTAGC 1 cut(s) 1445
BspPI GGATC 2 cut(s) 94, 1700
BspT104I TTCGAA 2 cut(s) 356, 2014
BspT107I GGYRCC 3 cut(s) 116, 870, 2267
BsrBI CCGCTC 1 cut(s) 1771
BsrDI GCAATG 2 cut(s) 1393, 1781
BsrFI RCCGGY 1 cut(s) 119
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 8 cut(s) 274, 508, 639, 1494, 1599, 2345, 2446, 2528
BssMI GATC 6 cut(s) 36, 99, 285, 967, 1692, 2149
BssNI GRCGYC 1 cut(s) 117
BssSI CACGAG 1 cut(s) 414
BssT1I CCWWGG 3 cut(s) 274, 1494, 2345
Bst2BI CACGAG 1 cut(s) 414
Bst2UI CCWGG 7 cut(s) 384, 510, 641, 837, 1600, 2448, 2529
Bst6I CTCTTC 1 cut(s) 2131
BstACI GRCGYC 1 cut(s) 117
BstAPI GCANNNNNTGC 1 cut(s) 1383
BstBAI YACGTR 1 cut(s) 941
BstBI TTCGAA 2 cut(s) 356, 2014
BstC8I GCNNGC 7 cut(s) 121, 345, 712, 716, 760, 1443, 1750
BstDEI CTNAG 9 cut(s) 192, 441, 672, 720, 768, 920, 1068, 1109, 1677
BstDSI CCRYGG 1 cut(s) 1494
BstENI CCTNNNNNAGG 1 cut(s) 227
BstF5I GGATG 6 cut(s) 72, 186, 313, 1174, 1993, 2205
BstFNI CGCG 2 cut(s) 59, 125
BstH2I RGCGCY 2 cut(s) 120, 139
BstHHI GCGC 4 cut(s) 61, 119, 125, 138
BstKTI GATC 6 cut(s) 39, 102, 288, 970, 1695, 2152
BstMAI GTCTC 6 cut(s) 368, 551, 1304, 1594, 1797, 2057
BstMBI GATC 6 cut(s) 36, 99, 285, 967, 1692, 2149
BstNI CCWGG 7 cut(s) 384, 510, 641, 837, 1600, 2448, 2529
BstNSI RCATGY 3 cut(s) 327, 1752, 2324
BstSCI CCNGG 9 cut(s) 382, 508, 639, 835, 1598, 2195, 2446, 2527, 2596
BstSFI CTRYAG 2 cut(s) 139, 1855
BstSLI GKGCMC 3 cut(s) 87, 862, 2535
BstUI CGCG 2 cut(s) 59, 125
BstV1I GCAGC 6 cut(s) 639, 1097, 1201, 1270, 1757, 2092
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 2 cut(s) 99, 2149
BstYI RGATCY 2 cut(s) 99, 2149
Bsu36I CCTNAGG 1 cut(s) 720
BsuI GTATCC 1 cut(s) 2388
BsuRI GGCC 6 cut(s) 70, 219, 279, 1207, 2445, 2485
BtgI CCRYGG 1 cut(s) 1494
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 6 cut(s) 72, 186, 313, 1174, 1993, 2205
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 5 cut(s) 415, 1443, 1708, 1853, 2533
BveI ACCTGC 2 cut(s) 269, 1010
Cac8I GCNNGC 7 cut(s) 121, 345, 712, 716, 760, 1443, 1750
CaiI CAGNNNCTG 1 cut(s) 560
CciI TCATGA 1 cut(s) 427
CfoI GCGC 4 cut(s) 61, 119, 125, 138
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 7 cut(s) 69, 162, 217, 812, 924, 1491, 2444
CseI GACGC 1 cut(s) 65
Csp6I GTAC 8 cut(s) 531, 848, 871, 1057, 1074, 2268, 2318, 2403
CspCI CAANNNNNGTGG 2 cut(s) 922, 957
CviQI GTAC 8 cut(s) 531, 848, 871, 1057, 1074, 2268, 2318, 2403
DdeI CTNAG 9 cut(s) 192, 441, 672, 720, 768, 920, 1068, 1109, 1677
DinI GGCGCC 1 cut(s) 118
DpnI GATC 6 cut(s) 38, 101, 287, 969, 1694, 2151
DpnII GATC 6 cut(s) 36, 99, 285, 967, 1692, 2149
DraIII CACNNNGTG 1 cut(s) 944
EaeI YGGCCR 1 cut(s) 1205
Eam1104I CTCTTC 1 cut(s) 2131
EarI CTCTTC 1 cut(s) 2131
Eco130I CCWWGG 3 cut(s) 274, 1494, 2345
Eco24I GRGCYC 2 cut(s) 236, 1524
Eco32I GATATC 1 cut(s) 1630
Eco47I GGWCC 4 cut(s) 162, 812, 924, 1491
Eco47III AGCGCT 1 cut(s) 137
Eco57I CTGAAG 3 cut(s) 53, 1041, 2232
Eco72I CACGTG 1 cut(s) 941
Eco81I CCTNAGG 1 cut(s) 720
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoO109I RGGNCCY 2 cut(s) 162, 924
EcoRI GAATTC 2 cut(s) 1025, 1576
EcoRII CCWGG 7 cut(s) 382, 508, 639, 835, 1598, 2446, 2527
EcoRV GATATC 1 cut(s) 1630
EcoT14I CCWWGG 3 cut(s) 274, 1494, 2345
EcoT38I GRGCYC 2 cut(s) 236, 1524
EgeI GGCGCC 1 cut(s) 118
EheI GGCGCC 1 cut(s) 118
ErhI CCWWGG 3 cut(s) 274, 1494, 2345
Esp3I CGTCTC 1 cut(s) 1797
FalI AAGNNNNNCTT 2 cut(s) 1043, 1075
FaqI GGGAC 6 cut(s) 148, 352, 1504, 1618, 1684, 1822
FauI CCCGC 4 cut(s) 415, 587, 719, 723
Fnu4HI GCNGC 9 cut(s) 115, 280, 628, 1086, 1190, 1284, 1746, 1772, 2081
FokI GGATG 6 cut(s) 59, 193, 320, 1181, 2000, 2212
FriOI GRGCYC 2 cut(s) 236, 1524
Fsp4HI GCNGC 9 cut(s) 115, 280, 628, 1086, 1190, 1284, 1746, 1772, 2081
GlaI GCGC 4 cut(s) 60, 118, 124, 137
GluI GCNGC 9 cut(s) 115, 280, 628, 1086, 1190, 1284, 1746, 1772, 2081
GsaI CCCAGC 2 cut(s) 175, 579
GsuI CTGGAG 3 cut(s) 209, 1313, 1582
HaeII RGCGCY 2 cut(s) 120, 139
HaeIII GGCC 6 cut(s) 70, 219, 279, 1207, 2445, 2485
HapII CCGG 3 cut(s) 120, 2196, 2597
HgaI GACGC 1 cut(s) 65
HhaI GCGC 4 cut(s) 61, 119, 125, 138
Hin1I GRCGYC 1 cut(s) 117
Hin6I GCGC 4 cut(s) 59, 117, 123, 136
HinP1I GCGC 4 cut(s) 59, 117, 123, 136
HincII GTYRAC 1 cut(s) 1508
HindII GTYRAC 1 cut(s) 1508
HinfI GANTC 8 cut(s) 208, 448, 469, 865, 1180, 1421, 1461, 2589
HpaII CCGG 3 cut(s) 120, 2196, 2597
HphI GGTGA 7 cut(s) 250, 536, 956, 1319, 1940, 2513, 2516
Hpy166II GTNNAC 6 cut(s) 85, 623, 885, 1057, 1508, 2028
Hpy188I TCNGA 6 cut(s) 157, 612, 916, 1634, 1678, 2154
Hpy188III TCNNGA 9 cut(s) 40, 205, 226, 428, 1759, 1919, 2136, 2218, 2460
Hpy8I GTNNAC 6 cut(s) 85, 623, 885, 1057, 1508, 2028
Hpy99I CGWCG 2 cut(s) 46, 242
HpyAV CCTTC 9 cut(s) 223, 1217, 1330, 1388, 1562, 1566, 2010, 2364, 2574
HpyCH4IV ACGT 2 cut(s) 6, 940
HpyF3I CTNAG 9 cut(s) 192, 441, 672, 720, 768, 920, 1068, 1109, 1677
HpySE526I ACGT 2 cut(s) 6, 940
Hsp92I GRCGYC 1 cut(s) 117
HspAI GCGC 4 cut(s) 59, 117, 123, 136
KasI GGCGCC 1 cut(s) 116
KflI GGGWCCC 1 cut(s) 162
KpnI GGTACC 2 cut(s) 874, 2271
KroI GCCGGC 1 cut(s) 119
KroNI GCCGGC 1 cut(s) 121
Kzo9I GATC 6 cut(s) 36, 99, 285, 967, 1692, 2149
LmnI GCTCC 4 cut(s) 239, 838, 1768, 2300
Lsp1109I GCAGC 6 cut(s) 639, 1097, 1201, 1270, 1757, 2092
MaeII ACGT 2 cut(s) 6, 940
MalI GATC 6 cut(s) 38, 101, 287, 969, 1694, 2151
MbiI CCGCTC 1 cut(s) 1771
MboI GATC 6 cut(s) 36, 99, 285, 967, 1692, 2149
MboII GAAGA 7 cut(s) 46, 59, 525, 1034, 1943, 2003, 2118
MfeI CAATTG 2 cut(s) 1130, 2097
MflI RGATCY 2 cut(s) 99, 2149
MhlI GDGCHC 7 cut(s) 87, 236, 415, 862, 1524, 2305, 2535
Mly113I GGCGCC 1 cut(s) 117
MlyI GAGTC 3 cut(s) 442, 1470, 2598
MmeI TCCRAC 1 cut(s) 2305
MreI CGCCGGCG 1 cut(s) 119
MroNI GCCGGC 1 cut(s) 119
MroXI GAANNNNTTC 2 cut(s) 1576, 2010
MseI TTAA 7 cut(s) 402, 891, 960, 1008, 1449, 1653, 2166
MslI CAYNNNNRTG 2 cut(s) 1244, 1816
MspA1I CMGCKG 3 cut(s) 1088, 1192, 2249
MspI CCGG 3 cut(s) 120, 2196, 2597
MspR9I CCNGG 9 cut(s) 384, 510, 641, 837, 1600, 2197, 2448, 2529, 2598
MunI CAATTG 2 cut(s) 1130, 2097
Mva1269I GAATGC 3 cut(s) 364, 2260, 2513
MvaI CCWGG 7 cut(s) 384, 510, 641, 837, 1600, 2448, 2529
MvnI CGCG 2 cut(s) 59, 125
NaeI GCCGGC 1 cut(s) 121
NarI GGCGCC 1 cut(s) 117
NciI CCSGG 2 cut(s) 2197, 2598
NcoI CCATGG 1 cut(s) 1494
NdeII GATC 6 cut(s) 36, 99, 285, 967, 1692, 2149
NgoMIV GCCGGC 1 cut(s) 119
NheI GCTAGC 1 cut(s) 1441
NlaIV GGNNCC 9 cut(s) 118, 163, 164, 235, 645, 872, 1492, 2269, 2352
NmuCI GTSAC 5 cut(s) 690, 901, 997, 1850, 2519
NspI RCATGY 3 cut(s) 327, 1752, 2324
NspV TTCGAA 2 cut(s) 356, 2014
OliI CACNNNNGTG 1 cut(s) 1244
PaeI GCATGC 1 cut(s) 1752
PagI TCATGA 1 cut(s) 427
PaqCI CACCTGC 1 cut(s) 269
PciI ACATGT 2 cut(s) 323, 2320
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 3 cut(s) 364, 2260, 2513
PdiI GCCGGC 1 cut(s) 121
PdmI GAANNNNTTC 2 cut(s) 1576, 2010
PfeI GAWTC 5 cut(s) 208, 469, 865, 1180, 1421
PflMI CCANNNNNTGG 2 cut(s) 560, 2576
PkrI GCNGC 9 cut(s) 116, 281, 629, 1087, 1191, 1285, 1747, 1773, 2082
PleI GAGTC 3 cut(s) 442, 1469, 2597
PluTI GGCGCC 1 cut(s) 120
PmaCI CACGTG 1 cut(s) 941
PmlI CACGTG 1 cut(s) 941
PpsI GAGTC 3 cut(s) 442, 1469, 2597
Ppu21I YACGTR 1 cut(s) 941
PpuMI RGGWCCY 2 cut(s) 162, 924
PscI ACATGT 2 cut(s) 323, 2320
PsiI TTATAA 2 cut(s) 296, 1793
Psp5II RGGWCCY 2 cut(s) 162, 924
Psp6I CCWGG 7 cut(s) 382, 508, 639, 835, 1598, 2446, 2527
PspCI CACGTG 1 cut(s) 941
PspFI CCCAGC 2 cut(s) 171, 575
PspGI CCWGG 7 cut(s) 382, 508, 639, 835, 1598, 2446, 2527
PspN4I GGNNCC 9 cut(s) 118, 163, 164, 235, 645, 872, 1492, 2269, 2352
PspPI GGNCC 7 cut(s) 69, 162, 217, 812, 924, 1491, 2444
PspPPI RGGWCCY 2 cut(s) 162, 924
PstNI CAGNNNCTG 1 cut(s) 560
PsuI RGATCY 2 cut(s) 99, 2149
PvuII CAGCTG 2 cut(s) 1088, 2249
RsaI GTAC 8 cut(s) 532, 849, 872, 1058, 1075, 2269, 2319, 2404
RsaNI GTAC 8 cut(s) 531, 848, 871, 1057, 1074, 2268, 2318, 2403
RseI CAYNNNNRTG 2 cut(s) 1244, 1816
SaqAI TTAA 7 cut(s) 402, 891, 960, 1008, 1449, 1653, 2166
SatI GCNGC 9 cut(s) 115, 280, 628, 1086, 1190, 1284, 1746, 1772, 2081
Sau3AI GATC 6 cut(s) 36, 99, 285, 967, 1692, 2149
Sau96I GGNCC 7 cut(s) 69, 162, 217, 812, 924, 1491, 2444
SchI GAGTC 3 cut(s) 442, 1470, 2598
ScrFI CCNGG 9 cut(s) 384, 510, 641, 837, 1600, 2197, 2448, 2529, 2598
SduI GDGCHC 7 cut(s) 87, 236, 415, 862, 1524, 2305, 2535
SfcI CTRYAG 2 cut(s) 139, 1855
SfoI GGCGCC 1 cut(s) 118
SfuI TTCGAA 2 cut(s) 356, 2014
SgrAI CRCCGGYG 1 cut(s) 119
SinI GGWCC 4 cut(s) 162, 812, 924, 1491
SmiMI CAYNNNNRTG 2 cut(s) 1244, 1816
SmlI CTYRAG 2 cut(s) 1533, 1757
SmoI CTYRAG 2 cut(s) 1533, 1757
SpeI ACTAGT 1 cut(s) 1502
SphI GCATGC 1 cut(s) 1752
SspDI GGCGCC 1 cut(s) 116
StyD4I CCNGG 9 cut(s) 382, 508, 639, 835, 1598, 2195, 2446, 2527, 2596
StyI CCWWGG 3 cut(s) 274, 1494, 2345
TaiI ACGT 2 cut(s) 9, 943
TaqI TCGA 5 cut(s) 356, 1145, 1178, 2014, 2558
TatI WGTACW 4 cut(s) 530, 847, 1056, 2317
TauI GCSGC 3 cut(s) 117, 282, 1774
TfiI GAWTC 5 cut(s) 208, 469, 865, 1180, 1421
Tru1I TTAA 7 cut(s) 402, 891, 960, 1008, 1449, 1653, 2166
Tru9I TTAA 7 cut(s) 402, 891, 960, 1008, 1449, 1653, 2166
TscAI CASTG 5 cut(s) 415, 1443, 1708, 1853, 2540
TseFI GTSAC 5 cut(s) 690, 901, 997, 1850, 2519
TseI GCWGC 6 cut(s) 627, 1085, 1189, 1283, 1745, 2080
Tsp45I GTSAC 5 cut(s) 690, 901, 997, 1850, 2519
TspGWI ACGGA 4 cut(s) 32, 59, 226, 1828
TspRI CASTG 5 cut(s) 415, 1443, 1708, 1853, 2540
Van91I CCANNNNNTGG 2 cut(s) 560, 2576
VneI GTGCAC 1 cut(s) 83
VpaK11BI GGWCC 4 cut(s) 162, 812, 924, 1491
XagI CCTNNNNNAGG 1 cut(s) 227
XbaI TCTAGA 1 cut(s) 2135
XceI RCATGY 3 cut(s) 327, 1752, 2324
XmaJI CCTAGG 1 cut(s) 2345
XmnI GAANNNNTTC 2 cut(s) 1576, 2010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.