Rorug07G0142000

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0009053
Physical Location & Seq
Forward (+)
11191515 .. 11191844
330 bp
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UTR
Exon/CDS
Intron
Rorug07G0142000.1

Sequence Viewer

Length: 330 bp
ATGGGCACCCCAGAAAGCAGTTCTGGCACATCCAGCAGTCGGAGCTTTTGGACTCACTGCGGCTGGAATTCTACACTAGAGTGTGTTAGTGAAGGGGTTCCTATGATTTGTATGCCATGTTCTGCTGATCAAATGATAAATGCTCGATATGTGAGTGATGTTTGGACAGTTGGGCTGCAGTTGGATCAGGGAAGAGAAAGAGGTGAGATAGAGAAAACAATTAGAAAACTAATGGTGAAGAAAGAAGGTGAAGATATCAGAAAAAATATGTCAAACCTAAAGGAGAAGGCAGATCTTTGCTTCAGACCAGGTGGCTCATCGATCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

109

Amino Acids

12.09

Weight (kDa)

6.57

Isoelectric Point (pI)

48.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UDPGT PF00201 12 - 58 5.1e-12 UDP-glucoronosyl and UDP-glucosyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 5
AciI CCGC 1 cut(s) 60
AclWI GGATC 1 cut(s) 192
AcsI RAATTY 1 cut(s) 67
AcuI CTGAAG 1 cut(s) 286
AfiI CCNNNNNNNGG 1 cut(s) 39
AjnI CCWGG 1 cut(s) 307
AleI CACNNNNGTG 1 cut(s) 79
AluBI AGCT 1 cut(s) 45
AluI AGCT 1 cut(s) 45
AlwI GGATC 1 cut(s) 192
ApeKI GCWGC 1 cut(s) 175
ApoI RAATTY 1 cut(s) 67
Asp700I GAANNNNTTC 1 cut(s) 96
AsuHPI GGTGA 3 cut(s) 215, 247, 260
BaeGI GKGCMC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 5
BbvI GCAGC 1 cut(s) 162
BciT130I CCWGG 1 cut(s) 309
BclI TGATCA 1 cut(s) 127
BfaI CTAG 1 cut(s) 77
BfmI CTRYAG 1 cut(s) 176
BglII AGATCT 1 cut(s) 292
BisI GCNGC 2 cut(s) 61, 176
BlsI GCNGC 2 cut(s) 62, 177
Bme1390I CCNGG 1 cut(s) 309
BmiI GGNNCC 2 cut(s) 7, 99
BmrFI CCNGG 1 cut(s) 309
Bsa29I ATCGAT 1 cut(s) 320
Bsc4I CCNNNNNNNGG 1 cut(s) 39
BseBI CCWGG 1 cut(s) 309
BseCI ATCGAT 1 cut(s) 320
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 1 cut(s) 39
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 1 cut(s) 162
Bsh1285I CGRYCG 1 cut(s) 324
BshNI GGYRCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 320
BsiEI CGRYCG 1 cut(s) 324
BslI CCNNNNNNNGG 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 4 cut(s) 127, 184, 292, 321
BspACI CCGC 1 cut(s) 60
BspDI ATCGAT 1 cut(s) 320
BspLI GGNNCC 2 cut(s) 7, 99
BspMAI CTGCAG 1 cut(s) 180
BspPI GGATC 1 cut(s) 192
BspT107I GGYRCC 1 cut(s) 5
BssMI GATC 4 cut(s) 127, 184, 292, 321
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 1 cut(s) 169
Bst6I CTCTTC 1 cut(s) 187
BstF5I GGATG 1 cut(s) 29
BstKTI GATC 4 cut(s) 130, 187, 295, 324
BstMBI GATC 4 cut(s) 127, 184, 292, 321
BstMCI CGRYCG 1 cut(s) 324
BstMWI GCNNNNNNNGC 3 cut(s) 24, 33, 42
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 307
BstSFI CTRYAG 1 cut(s) 176
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
Bsu15I ATCGAT 1 cut(s) 320
BsuTUI ATCGAT 1 cut(s) 320
BtsCI GGATG 1 cut(s) 29
BtsI GCAGTG 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 55
ClaI ATCGAT 1 cut(s) 320
CsiI ACCWGGT 1 cut(s) 307
CviAII CATG 1 cut(s) 117
CviJI RGCY 4 cut(s) 45, 63, 175, 315
CviKI_1 RGCY 4 cut(s) 45, 63, 175, 315
DpnI GATC 4 cut(s) 129, 186, 294, 323
DpnII GATC 4 cut(s) 127, 184, 292, 321
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
Eco32I GATATC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 286
EcoRI GAATTC 1 cut(s) 67
EcoRII CCWGG 1 cut(s) 307
EcoRV GATATC 1 cut(s) 256
FaeI CATG 1 cut(s) 120
FaiI YATR 6 cut(s) 104, 113, 118, 150, 269, 328
FatI CATG 1 cut(s) 116
FbaI TGATCA 1 cut(s) 127
Fnu4HI GCNGC 2 cut(s) 61, 176
FokI GGATG 1 cut(s) 16
Fsp4HI GCNGC 2 cut(s) 61, 176
FspBI CTAG 1 cut(s) 77
GluI GCNGC 2 cut(s) 61, 176
Hin1II CATG 1 cut(s) 120
HinfI GANTC 1 cut(s) 52
HphI GGTGA 3 cut(s) 215, 247, 260
Hpy188I TCNGA 3 cut(s) 42, 260, 305
HpyAV CCTTC 3 cut(s) 86, 239, 280
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4V TGCA 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 33, 42
Hsp92II CATG 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 4 cut(s) 127, 184, 292, 321
LmnI GCTCC 1 cut(s) 42
LpnPI CCDG 7 cut(s) 9, 24, 46, 49, 173, 294, 321
Lsp1109I GCAGC 1 cut(s) 162
MabI ACCWGGT 1 cut(s) 307
MaeI CTAG 1 cut(s) 77
MalI GATC 4 cut(s) 129, 186, 294, 323
MboI GATC 4 cut(s) 127, 184, 292, 321
MboII GAAGA 3 cut(s) 204, 250, 263
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 2 cut(s) 67, 219
MlyI GAGTC 1 cut(s) 46
MmeI TCCRAC 2 cut(s) 20, 162
MnlI CCTC 1 cut(s) 194
MroXI GAANNNNTTC 1 cut(s) 96
MslI CAYNNNNRTG 1 cut(s) 79
MspR9I CCNGG 1 cut(s) 309
MvaI CCWGG 1 cut(s) 309
MwoI GCNNNNNNNGC 3 cut(s) 24, 33, 42
NdeII GATC 4 cut(s) 127, 184, 292, 321
NlaIII CATG 1 cut(s) 120
NlaIV GGNNCC 2 cut(s) 7, 99
OliI CACNNNNGTG 1 cut(s) 79
PdmI GAANNNNTTC 1 cut(s) 96
PkrI GCNGC 2 cut(s) 62, 177
Ple19I CGATCG 1 cut(s) 324
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 2 cut(s) 7, 99
PsrI GAACNNNNNNTAC 2 cut(s) 103, 135
PstI CTGCAG 1 cut(s) 180
PsuI RGATCY 1 cut(s) 292
PvuI CGATCG 1 cut(s) 324
RseI CAYNNNNRTG 1 cut(s) 79
SatI GCNGC 2 cut(s) 61, 176
Sau3AI GATC 4 cut(s) 127, 184, 292, 321
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 1 cut(s) 8
SetI ASST 5 cut(s) 47, 205, 250, 279, 313
SexAI ACCWGGT 1 cut(s) 307
SfcI CTRYAG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 79
Sse9I AATT 2 cut(s) 67, 219
SsiI CCGC 1 cut(s) 60
SspMI CTAG 1 cut(s) 77
StyD4I CCNGG 1 cut(s) 307
TaaI ACNGT 1 cut(s) 169
TaqI TCGA 2 cut(s) 145, 320
TasI AATT 2 cut(s) 67, 219
TauI GCSGC 1 cut(s) 63
TscAI CASTG 1 cut(s) 62
TseI GCWGC 1 cut(s) 175
TspRI CASTG 1 cut(s) 62
XapI RAATTY 1 cut(s) 67
XmnI GAANNNNTTC 1 cut(s) 96
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.