Rw0G012030

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00535
OG0009053
Physical Location & Seq
Forward (+)
42963 .. 47464
4502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G012030.1

Sequence Viewer

Length: 2421 bp
ATGCCTATAACTATCCACTCACAGGGCGGCGCCGGCGCCGACAAAGAGCGCTACAGGCTGGCCTTGTCCGATGGGTCCCACACCCAGCAGGGGATGCTTGCCACTCAGCAGAACTTCCTGATTCGGCAGGGCCACCTCCAGGTGGGCTCCGTCGTCTGCCTCAATCAATTCACCTGCACCACCGTCCAAGGCCGCCAGATCATCATTATAATCTCATTGGATGTGCTAGTTGATACATGTGAAATCATTGGAGAGCCTGCCAGAATTTCGAATGCCCAGTCCCCAGGGACAATACCTGCAAATGCTCAATCTCTTAACAGCAGTGCTCGTGTGGCGGGTCATGGTTCTTATTCTGGTAGTGCTGACCCAGGAAGATATGCTACAATGAGTACAGTTCCCAATCACCCTAGACCAGAGACTGGTTCTGGTTTTGCTGGGTTAGCACCAGTAAGTGGGTCATATGCTGGTCAGAACACAGGTTTACGCAGCCTCATATCCCAGGTTCCACCTCCACTTCGAAATTCTTATGCTCAGCCGACTTATCAACAGTCGCACTCAAATTCTTATGCCCGCCCGCCTCAGGCAACATATCAACAGCCACCTCCAAGTTCTTATGCACGCCCACCTCAGCCAACTCATCAACAGCCACCTCCATTATATAGTAACAGAGGACCAGTTGCCAAAAATGAAGCTCCTGGTGGAATAGTACAAATTTGTGCCCTGAATCGGTACCTAAATAAGTGGACAATTAAGGCTAGAGTGACGGCTAAATCAGAACTATCAGAACTCAGGTCCTACACCAATGCACGTGGTGAGGGGAAAGTATTTAATTTTGATCTCATTGATTGTGGTGGGGAAATTCGTGTGACCTGCTTTAATCCAATAGCTGAAGAATTCTACAATTTGATTGAAGTTGGTAAGATGTACTTGATTTCTAAGGGTACAGTAAAGGCAGCTGTAAAGAAATTCAACCACCTTAGTAACGAGTTTGAAATTCAATTGGACACCACTTCGACAATACAGTTATGTTTTGAGGATGATGGCTCGATTCCACAGCAGCGGTTCAATTTTCGGCCAATAAGTGATGTTGAAGGTTTGGAGAACAACACTATTGTGGATATAATAGGTGTGGTAACATTCATCAGTCCTGCTGCTTCAATAAGCACGAAAAACGGTGAGACACAAAAGAGAACTCTCCAGTTGAAGGATATGTCTTGTCGTAGTGTTGAAGTAACTCTGTGGGGCAATTTGTGCAATGCAGAAGGGCAAAAACTTCAAAGTATGTGCGATTCTGGGTTATTTCCAGTGCTAGCTGTTAAAGCTGGTAGAGTCAATGACTTCAATGGCAAGGGGGTGGGGACCATGGCTACTAGTCAACTCTTTATAGAGCCCGATAACTCTCAAGCTGATGAGGTGAGGGAATGGTTTGACAGGGAAGGAAGGAATTCCCCATGTGTCTCTATCTCCAGGGGGACAGCAAGTGTGGGGAGAAATGATATCCGAAAGACTATATCCCAGATTAAAGATGAGAAGCTAGGGACTTCTGAGAAGCCAGATTGGATCACAATCAGTGCTACTGTCATATTTGTAAAGAGTGATAGCTACTTCTATGCAGCATGCCCACTCAAGATAGGGGAGCGGCAATGCAACAAAAAGGTTATAAATGATGGAGACGGGACATGGCGGTGTGAAAGATGTGAGCAGTCCGTTGAACAGTGTGACTACAGATATATACTCAACTTACAGATACAAGACCATACTGGCATAGCATGGGTAACTGCATTTCAGGAGGCTGGTGAAGAAATAATGGGTATAACTGCAAAAGATTTTCATAATTTGAAATATGAAATGCAGGATGAAGATAAAGTTGCAGAAATCGTTCGAAGGGTCTTGTTTACTGAATTTGTTTTCAAGTTGAAAATAAAGGAGGAGACTTTTAGTGATGAGCAGCGTGTGAAATCAACAATTGTCAAAGCAGATAAAGTTAGTTTCTCTTCAAAATCTAGATTTCATTTAGATCTGATGGATAGACTTAAAAATGGGGAAACTGCAATACCTTGTGCCGGGATGAACAATACTGAAGTCGGGAGTTCTGCAATTAGACAACATACACCAGCTGTGAATGCTAGTTACGGTACCAATGCTGTCAATGCTGGTAGAGAGTTTGGAGCACCAGCAAATCAAGTACATGTTGGAAGCCAGTATATTAGTCCTAGGGTTCCTTCAACTGGCTCTGCTGGTTTGTATCCTACCTGTAACATCTGTGGAGGTACAAGCCATAATTCTTTGAACTGCCCAAATGTTATGAATGGCCCAGGACAGTCATCAGGAGGGGGTTACACCAATAGTAGGCCAGTTGCCAGTAATTCTGGTGAATGCTATAAATGTCACCAACCTGGGCCGGGCGAAAGACTGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

806

Amino Acids

87.9

Weight (kDa)

7.73

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 11 - 77 1.3e-16 Replication factor-A protein 1, N-terminal domain
tRNA_anti-codon PF01336 248 - 334 4.3e-09 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 360 - 462 1.7e-25 Replication protein A OB domain
Rep_fac-A_C PF08646 520 - 669 1e-49 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 209, 1661
AarI CACCTGC 1 cut(s) 182
Acc36I ACCTGC 3 cut(s) 182, 304, 878
Acc65I GGTACC 2 cut(s) 729, 2135
AccB1I GGYRCC 4 cut(s) 29, 35, 729, 2135
AccB7I CCANNNNNTGG 2 cut(s) 419, 452
AccBSI CCGCTC 1 cut(s) 1639
AciI CCGC 8 cut(s) 27, 193, 335, 571, 575, 1060, 1639, 1684
AclWI GGATC 1 cut(s) 1568
AcoI YGGCCR 1 cut(s) 1073
AcuI CTGAAG 2 cut(s) 909, 2100
AcvI CACGTG 1 cut(s) 809
AcyI GRCGYC 2 cut(s) 30, 36
AdeI CACNNNGTG 1 cut(s) 812
AfaI GTAC 8 cut(s) 391, 708, 731, 926, 943, 2137, 2187, 2272
AfeI AGCGCT 1 cut(s) 50
AflIII ACRYGT 2 cut(s) 236, 2188
AhlI ACTAGT 1 cut(s) 1370
AjnI CCWGG 8 cut(s) 138, 283, 367, 498, 694, 1466, 2314, 2395
AleI CACNNNNGTG 1 cut(s) 1112
AluBI AGCT 9 cut(s) 692, 887, 956, 1313, 1322, 1406, 1534, 1602, 2117
AluI AGCT 9 cut(s) 692, 887, 956, 1313, 1322, 1406, 1534, 1602, 2117
Alw21I GWGCWC 2 cut(s) 328, 2173
Alw26I GTCTC 5 cut(s) 410, 1172, 1462, 1665, 1925
AlwI GGATC 1 cut(s) 1568
AlwNI CAGNNNCTG 1 cut(s) 419
Aor51HI AGCGCT 1 cut(s) 50
AoxI GGCC 7 cut(s) 60, 130, 190, 1073, 2311, 2351, 2399
ApeKI GCWGC 6 cut(s) 486, 953, 1057, 1151, 1613, 1948
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
Asp700I GAANNNNTTC 2 cut(s) 1444, 1878
Asp718I GGTACC 2 cut(s) 729, 2135
AspA2I CCTAGG 1 cut(s) 2213
AspLEI GCGC 3 cut(s) 32, 38, 51
AspS9I GGNCC 7 cut(s) 75, 130, 671, 792, 1359, 2312, 2399
AsuC2I CCSGG 2 cut(s) 2065, 2403
AsuHPI GGTGA 8 cut(s) 163, 395, 824, 1187, 1426, 1808, 2381, 2384
AsuII TTCGAA 3 cut(s) 269, 517, 1882
AsuNHI GCTAGC 1 cut(s) 1309
AvaII GGWCC 4 cut(s) 75, 671, 792, 1359
AvrII CCTAGG 1 cut(s) 2213
AxyI CCTNAGG 1 cut(s) 579
BaeGI GKGCMC 1 cut(s) 721
BanI GGYRCC 4 cut(s) 29, 35, 729, 2135
BanII GRGCYC 2 cut(s) 149, 1392
BarI GAAGNNNNNNTAC 2 cut(s) 364, 396
BauI CACGAG 1 cut(s) 327
BbrPI CACGTG 1 cut(s) 809
Bbv12I GWGCWC 2 cut(s) 328, 2173
BbvCI CCTCAGC 1 cut(s) 627
BbvI GCAGC 6 cut(s) 498, 965, 1069, 1138, 1625, 1960
BccI CCATC 4 cut(s) 65, 1034, 1661, 2017
BceAI ACGGC 1 cut(s) 780
BciT130I CCWGG 8 cut(s) 140, 285, 369, 500, 696, 1468, 2316, 2397
BciVI GTATCC 1 cut(s) 2256
BcnI CCSGG 2 cut(s) 2065, 2403
BcoDI GTCTC 5 cut(s) 410, 1172, 1462, 1665, 1925
BcuI ACTAGT 1 cut(s) 1370
BfmI CTRYAG 2 cut(s) 52, 1723
BfoI RGCGCY 3 cut(s) 33, 39, 52
BfuAI ACCTGC 3 cut(s) 182, 304, 878
BfuI GTATCC 1 cut(s) 2256
BglII AGATCT 1 cut(s) 2017
BisI GCNGC 9 cut(s) 28, 193, 487, 954, 1058, 1152, 1614, 1640, 1949
BlnI CCTAGG 1 cut(s) 2213
BlpI GCTNAGC 1 cut(s) 531
BlsI GCNGC 9 cut(s) 29, 194, 488, 955, 1059, 1153, 1615, 1641, 1950
Bme18I GGWCC 4 cut(s) 75, 671, 792, 1359
BmgT120I GGNCC 7 cut(s) 75, 130, 671, 792, 1359, 2312, 2399
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 1 cut(s) 84
BmtI GCTAGC 1 cut(s) 1313
BmuI ACTGGG 1 cut(s) 271
BpmI CTGGAG 3 cut(s) 122, 1181, 1450
Bpu10I CCTNAGC 1 cut(s) 627
Bpu1102I GCTNAGC 1 cut(s) 531
Bpu14I TTCGAA 3 cut(s) 269, 517, 1882
BpuEI CTTGAG 2 cut(s) 1386, 1610
BpuMI CCSGG 2 cut(s) 2065, 2403
BsaAI YACGTR 1 cut(s) 809
BsaBI GATNNNNATC 1 cut(s) 1565
BsaHI GRCGYC 2 cut(s) 30, 36
Bse118I RCCGGY 1 cut(s) 32
Bse21I CCTNAGG 1 cut(s) 579
Bse3DI GCAATG 2 cut(s) 1261, 1649
Bse8I GATNNNNATC 1 cut(s) 1565
BseBI CCWGG 8 cut(s) 140, 285, 369, 500, 696, 1468, 2316, 2397
BseGI GGATG 5 cut(s) 99, 226, 1042, 1861, 2073
BseJI GATNNNNATC 1 cut(s) 1565
BseMI GCAATG 2 cut(s) 1261, 1649
BseMII CTCAG 6 cut(s) 119, 545, 593, 641, 802, 1536
BseRI GAGGAG 1 cut(s) 1943
BseSI GKGCMC 1 cut(s) 721
BseXI GCAGC 6 cut(s) 498, 965, 1069, 1138, 1625, 1960
BseYI CCCAGC 2 cut(s) 84, 434
BsgI GTGCAG 1 cut(s) 160
BshFI GGCC 7 cut(s) 62, 132, 192, 1075, 2313, 2353, 2401
BshNI GGYRCC 4 cut(s) 29, 35, 729, 2135
BsiHKAI GWGCWC 2 cut(s) 328, 2173
BsiSI CCGG 3 cut(s) 33, 2064, 2402
BslFI GGGAC 7 cut(s) 61, 265, 301, 1372, 1486, 1552, 1690
BsmAI GTCTC 5 cut(s) 410, 1172, 1462, 1665, 1925
BsmBI CGTCTC 1 cut(s) 1665
BsmFI GGGAC 7 cut(s) 61, 265, 301, 1372, 1486, 1552, 1690
BsmI GAATGC 3 cut(s) 277, 2128, 2381
BsnI GGCC 7 cut(s) 62, 132, 192, 1075, 2313, 2353, 2401
Bsp119I TTCGAA 3 cut(s) 269, 517, 1882
Bsp1286I GDGCHC 5 cut(s) 149, 328, 721, 1392, 2173
Bsp143I GATC 4 cut(s) 198, 835, 1560, 2017
Bsp1720I GCTNAGC 1 cut(s) 531
Bsp19I CCATGG 1 cut(s) 1362
BspACI CCGC 8 cut(s) 27, 193, 335, 571, 575, 1060, 1639, 1684
BspANI GGCC 7 cut(s) 62, 132, 192, 1075, 2313, 2353, 2401
BspCNI CTCAG 6 cut(s) 118, 544, 592, 640, 801, 1537
BspMI ACCTGC 3 cut(s) 182, 304, 878
BspOI GCTAGC 1 cut(s) 1313
BspPI GGATC 1 cut(s) 1568
BspT104I TTCGAA 3 cut(s) 269, 517, 1882
BspT107I GGYRCC 4 cut(s) 29, 35, 729, 2135
BsrBI CCGCTC 1 cut(s) 1639
BsrDI GCAATG 2 cut(s) 1261, 1649
BsrFI RCCGGY 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 32
BssMI GATC 4 cut(s) 198, 835, 1560, 2017
BssNI GRCGYC 2 cut(s) 30, 36
BssSI CACGAG 1 cut(s) 327
BssT1I CCWWGG 3 cut(s) 187, 1362, 2213
Bst2BI CACGAG 1 cut(s) 327
Bst2UI CCWGG 8 cut(s) 140, 285, 369, 500, 696, 1468, 2316, 2397
Bst6I CTCTTC 1 cut(s) 1999
BstACI GRCGYC 2 cut(s) 30, 36
BstAPI GCANNNNNTGC 2 cut(s) 94, 1251
BstBAI YACGTR 1 cut(s) 809
BstBI TTCGAA 3 cut(s) 269, 517, 1882
BstC8I GCNNGC 9 cut(s) 34, 60, 99, 258, 571, 575, 619, 1311, 1618
BstDEI CTNAG 8 cut(s) 105, 531, 579, 627, 788, 936, 977, 1545
BstDSI CCRYGG 1 cut(s) 1362
BstF5I GGATG 5 cut(s) 99, 226, 1042, 1861, 2073
BstH2I RGCGCY 3 cut(s) 33, 39, 52
BstHHI GCGC 3 cut(s) 32, 38, 51
BstKTI GATC 4 cut(s) 201, 838, 1563, 2020
BstMAI GTCTC 5 cut(s) 410, 1172, 1462, 1665, 1925
BstMBI GATC 4 cut(s) 198, 835, 1560, 2017
BstMWI GCNNNNNNNGC 9 cut(s) 33, 55, 94, 332, 440, 1251, 1319, 2123, 2150
BstNI CCWGG 8 cut(s) 140, 285, 369, 500, 696, 1468, 2316, 2397
BstNSI RCATGY 3 cut(s) 240, 1620, 2192
BstSFI CTRYAG 2 cut(s) 52, 1723
BstSLI GKGCMC 1 cut(s) 721
BstV1I GCAGC 6 cut(s) 498, 965, 1069, 1138, 1625, 1960
BstX2I RGATCY 1 cut(s) 2017
BstYI RGATCY 1 cut(s) 2017
Bsu36I CCTNAGG 1 cut(s) 579
BsuI GTATCC 1 cut(s) 2256
BsuRI GGCC 7 cut(s) 62, 132, 192, 1075, 2313, 2353, 2401
BtgI CCRYGG 1 cut(s) 1362
BtsCI GGATG 5 cut(s) 99, 226, 1042, 1861, 2073
BtsI GCAGTG 1 cut(s) 328
BtsIMutI CAGTG 4 cut(s) 328, 1311, 1576, 1721
BveI ACCTGC 3 cut(s) 182, 304, 878
Cac8I GCNNGC 9 cut(s) 34, 60, 99, 258, 571, 575, 619, 1311, 1618
CaiI CAGNNNCTG 1 cut(s) 419
CfoI GCGC 3 cut(s) 32, 38, 51
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 7 cut(s) 75, 130, 671, 792, 1359, 2312, 2399
Csp6I GTAC 8 cut(s) 390, 707, 730, 925, 942, 2136, 2186, 2271
CspCI CAANNNNNGTGG 2 cut(s) 790, 825
CviAII CATG 8 cut(s) 237, 341, 1363, 1452, 1617, 1680, 1770, 2189
CviQI GTAC 8 cut(s) 390, 707, 730, 925, 942, 2136, 2186, 2271
DdeI CTNAG 8 cut(s) 105, 531, 579, 627, 788, 936, 977, 1545
DinI GGCGCC 2 cut(s) 31, 37
DpnI GATC 4 cut(s) 200, 837, 1562, 2019
DpnII GATC 4 cut(s) 198, 835, 1560, 2017
DraIII CACNNNGTG 1 cut(s) 812
EaeI YGGCCR 1 cut(s) 1073
Eam1104I CTCTTC 1 cut(s) 1999
EarI CTCTTC 1 cut(s) 1999
Eco130I CCWWGG 3 cut(s) 187, 1362, 2213
Eco24I GRGCYC 2 cut(s) 149, 1392
Eco32I GATATC 1 cut(s) 1498
Eco47I GGWCC 4 cut(s) 75, 671, 792, 1359
Eco47III AGCGCT 1 cut(s) 50
Eco57I CTGAAG 2 cut(s) 909, 2100
Eco72I CACGTG 1 cut(s) 809
Eco81I CCTNAGG 1 cut(s) 579
EcoO109I RGGNCCY 2 cut(s) 75, 792
EcoRI GAATTC 2 cut(s) 893, 1444
EcoRII CCWGG 8 cut(s) 138, 283, 367, 498, 694, 1466, 2314, 2395
EcoRV GATATC 1 cut(s) 1498
EcoT14I CCWWGG 3 cut(s) 187, 1362, 2213
EcoT38I GRGCYC 2 cut(s) 149, 1392
EgeI GGCGCC 2 cut(s) 31, 37
EheI GGCGCC 2 cut(s) 31, 37
ErhI CCWWGG 3 cut(s) 187, 1362, 2213
Esp3I CGTCTC 1 cut(s) 1665
FaeI CATG 8 cut(s) 240, 344, 1366, 1455, 1620, 1683, 1773, 2192
FalI AAGNNNNNCTT 2 cut(s) 911, 943
FaqI GGGAC 7 cut(s) 61, 265, 301, 1372, 1486, 1552, 1690
FatI CATG 8 cut(s) 236, 340, 1362, 1451, 1616, 1679, 1769, 2188
FauI CCCGC 3 cut(s) 328, 578, 582
FauNDI CATATG 1 cut(s) 460
Fnu4HI GCNGC 9 cut(s) 28, 193, 487, 954, 1058, 1152, 1614, 1640, 1949
FokI GGATG 5 cut(s) 106, 233, 1049, 1868, 2080
FriOI GRGCYC 2 cut(s) 149, 1392
Fsp4HI GCNGC 9 cut(s) 28, 193, 487, 954, 1058, 1152, 1614, 1640, 1949
GlaI GCGC 3 cut(s) 31, 37, 50
GluI GCNGC 9 cut(s) 28, 193, 487, 954, 1058, 1152, 1614, 1640, 1949
GsaI CCCAGC 2 cut(s) 88, 438
GsuI CTGGAG 3 cut(s) 122, 1181, 1450
HaeII RGCGCY 3 cut(s) 33, 39, 52
HaeIII GGCC 7 cut(s) 62, 132, 192, 1075, 2313, 2353, 2401
HapII CCGG 3 cut(s) 33, 2064, 2402
HhaI GCGC 3 cut(s) 32, 38, 51
Hin1I GRCGYC 2 cut(s) 30, 36
Hin1II CATG 8 cut(s) 240, 344, 1366, 1455, 1620, 1683, 1773, 2192
Hin6I GCGC 3 cut(s) 30, 36, 49
HinP1I GCGC 3 cut(s) 30, 36, 49
HincII GTYRAC 1 cut(s) 1376
HindII GTYRAC 1 cut(s) 1376
HinfI GANTC 5 cut(s) 121, 724, 1048, 1289, 1329
HpaII CCGG 3 cut(s) 33, 2064, 2402
HphI GGTGA 8 cut(s) 163, 395, 824, 1187, 1426, 1808, 2381, 2384
Hpy166II GTNNAC 4 cut(s) 482, 744, 1376, 1896
Hpy188I TCNGA 7 cut(s) 70, 471, 775, 784, 1502, 1546, 2022
Hpy188III TCNNGA 6 cut(s) 118, 1627, 1787, 2004, 2086, 2328
Hpy8I GTNNAC 4 cut(s) 482, 744, 1376, 1896
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 7 cut(s) 1085, 1198, 1256, 1430, 1434, 1878, 2232
HpyCH4IV ACGT 1 cut(s) 808
HpyF10VI GCNNNNNNNGC 9 cut(s) 33, 55, 94, 332, 440, 1251, 1319, 2123, 2150
HpyF3I CTNAG 8 cut(s) 105, 531, 579, 627, 788, 936, 977, 1545
HpySE526I ACGT 1 cut(s) 808
Hsp92I GRCGYC 2 cut(s) 30, 36
Hsp92II CATG 8 cut(s) 240, 344, 1366, 1455, 1620, 1683, 1773, 2192
HspAI GCGC 3 cut(s) 30, 36, 49
KasI GGCGCC 2 cut(s) 29, 35
KflI GGGWCCC 1 cut(s) 75
KpnI GGTACC 2 cut(s) 733, 2139
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
Kzo9I GATC 4 cut(s) 198, 835, 1560, 2017
LmnI GCTCC 4 cut(s) 152, 697, 1636, 2168
Lsp1109I GCAGC 6 cut(s) 498, 965, 1069, 1138, 1625, 1960
LweI GCATC 1 cut(s) 84
MaeII ACGT 1 cut(s) 808
MalI GATC 4 cut(s) 200, 837, 1562, 2019
MbiI CCGCTC 1 cut(s) 1639
MboI GATC 4 cut(s) 198, 835, 1560, 2017
MboII GAAGA 5 cut(s) 384, 902, 1811, 1871, 1986
MfeI CAATTG 2 cut(s) 998, 1965
MflI RGATCY 1 cut(s) 2017
MhlI GDGCHC 5 cut(s) 149, 328, 721, 1392, 2173
Mly113I GGCGCC 2 cut(s) 30, 36
MlyI GAGTC 1 cut(s) 1338
MmeI TCCRAC 1 cut(s) 2173
MreI CGCCGGCG 1 cut(s) 32
MroNI GCCGGC 1 cut(s) 32
MroXI GAANNNNTTC 2 cut(s) 1444, 1878
MseI TTAA 7 cut(s) 315, 750, 828, 876, 1317, 1521, 2034
MslI CAYNNNNRTG 2 cut(s) 1112, 1684
MspA1I CMGCKG 3 cut(s) 956, 1060, 2117
MspI CCGG 3 cut(s) 33, 2064, 2402
MunI CAATTG 2 cut(s) 998, 1965
Mva1269I GAATGC 3 cut(s) 277, 2128, 2381
MvaI CCWGG 8 cut(s) 140, 285, 369, 500, 696, 1468, 2316, 2397
MwoI GCNNNNNNNGC 9 cut(s) 33, 55, 94, 332, 440, 1251, 1319, 2123, 2150
NaeI GCCGGC 1 cut(s) 34
NarI GGCGCC 2 cut(s) 30, 36
NciI CCSGG 2 cut(s) 2065, 2403
NcoI CCATGG 1 cut(s) 1362
NdeI CATATG 1 cut(s) 460
NdeII GATC 4 cut(s) 198, 835, 1560, 2017
NgoMIV GCCGGC 1 cut(s) 32
NheI GCTAGC 1 cut(s) 1309
NlaIII CATG 8 cut(s) 240, 344, 1366, 1455, 1620, 1683, 1773, 2192
NmuCI GTSAC 4 cut(s) 760, 865, 1718, 2387
NspI RCATGY 3 cut(s) 240, 1620, 2192
NspV TTCGAA 3 cut(s) 269, 517, 1882
OliI CACNNNNGTG 1 cut(s) 1112
PaeI GCATGC 1 cut(s) 1620
PaqCI CACCTGC 1 cut(s) 182
PasI CCCWGGG 1 cut(s) 284
PciI ACATGT 2 cut(s) 236, 2188
PctI GAATGC 3 cut(s) 277, 2128, 2381
PdiI GCCGGC 1 cut(s) 34
PdmI GAANNNNTTC 2 cut(s) 1444, 1878
PfeI GAWTC 4 cut(s) 121, 724, 1048, 1289
PflMI CCANNNNNTGG 2 cut(s) 419, 452
PkrI GCNGC 9 cut(s) 29, 194, 488, 955, 1059, 1153, 1615, 1641, 1950
PleI GAGTC 1 cut(s) 1337
PluTI GGCGCC 2 cut(s) 33, 39
PmaCI CACGTG 1 cut(s) 809
PmlI CACGTG 1 cut(s) 809
PpsI GAGTC 1 cut(s) 1337
Ppu21I YACGTR 1 cut(s) 809
PpuMI RGGWCCY 2 cut(s) 75, 792
PscI ACATGT 2 cut(s) 236, 2188
PsiI TTATAA 2 cut(s) 209, 1661
Psp5II RGGWCCY 2 cut(s) 75, 792
Psp6I CCWGG 8 cut(s) 138, 283, 367, 498, 694, 1466, 2314, 2395
PspCI CACGTG 1 cut(s) 809
PspFI CCCAGC 2 cut(s) 84, 434
PspGI CCWGG 8 cut(s) 138, 283, 367, 498, 694, 1466, 2314, 2395
PspPI GGNCC 7 cut(s) 75, 130, 671, 792, 1359, 2312, 2399
PspPPI RGGWCCY 2 cut(s) 75, 792
PstNI CAGNNNCTG 1 cut(s) 419
PsuI RGATCY 1 cut(s) 2017
PvuII CAGCTG 2 cut(s) 956, 2117
RsaI GTAC 8 cut(s) 391, 708, 731, 926, 943, 2137, 2187, 2272
RsaNI GTAC 8 cut(s) 390, 707, 730, 925, 942, 2136, 2186, 2271
RseI CAYNNNNRTG 2 cut(s) 1112, 1684
SaqAI TTAA 7 cut(s) 315, 750, 828, 876, 1317, 1521, 2034
SatI GCNGC 9 cut(s) 28, 193, 487, 954, 1058, 1152, 1614, 1640, 1949
Sau3AI GATC 4 cut(s) 198, 835, 1560, 2017
Sau96I GGNCC 7 cut(s) 75, 130, 671, 792, 1359, 2312, 2399
SchI GAGTC 1 cut(s) 1338
SduI GDGCHC 5 cut(s) 149, 328, 721, 1392, 2173
SfaNI GCATC 1 cut(s) 84
SfcI CTRYAG 2 cut(s) 52, 1723
SfoI GGCGCC 2 cut(s) 31, 37
SfuI TTCGAA 3 cut(s) 269, 517, 1882
SgrAI CRCCGGYG 1 cut(s) 32
SinI GGWCC 4 cut(s) 75, 671, 792, 1359
SmiMI CAYNNNNRTG 2 cut(s) 1112, 1684
SmlI CTYRAG 2 cut(s) 1401, 1625
SmoI CTYRAG 2 cut(s) 1401, 1625
SpeI ACTAGT 1 cut(s) 1370
SphI GCATGC 1 cut(s) 1620
SsiI CCGC 8 cut(s) 27, 193, 335, 571, 575, 1060, 1639, 1684
SspDI GGCGCC 2 cut(s) 29, 35
StyI CCWWGG 3 cut(s) 187, 1362, 2213
TaiI ACGT 1 cut(s) 811
TaqI TCGA 5 cut(s) 269, 517, 1013, 1046, 1882
TatI WGTACW 4 cut(s) 389, 706, 924, 2185
TauI GCSGC 3 cut(s) 30, 195, 1642
TfiI GAWTC 4 cut(s) 121, 724, 1048, 1289
Tru1I TTAA 7 cut(s) 315, 750, 828, 876, 1317, 1521, 2034
Tru9I TTAA 7 cut(s) 315, 750, 828, 876, 1317, 1521, 2034
TscAI CASTG 4 cut(s) 328, 1311, 1576, 1721
TseFI GTSAC 4 cut(s) 760, 865, 1718, 2387
TseI GCWGC 6 cut(s) 486, 953, 1057, 1151, 1613, 1948
Tsp45I GTSAC 4 cut(s) 760, 865, 1718, 2387
TspDTI ATGAA 8 cut(s) 702, 1129, 1820, 1860, 1872, 2000, 2084, 2321
TspGWI ACGGA 2 cut(s) 139, 1696
TspRI CASTG 4 cut(s) 328, 1311, 1576, 1721
Van91I CCANNNNNTGG 2 cut(s) 419, 452
VpaK11BI GGWCC 4 cut(s) 75, 671, 792, 1359
XbaI TCTAGA 1 cut(s) 2003
XceI RCATGY 3 cut(s) 240, 1620, 2192
XmaJI CCTAGG 1 cut(s) 2213
XmnI GAANNNNTTC 2 cut(s) 1444, 1878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.