Rw7G023720

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009053
Physical Location & Seq
Reverse (-)
28504908 .. 28507962
3055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G023720.1

Sequence Viewer

Length: 2583 bp
ATGGACGTGAGGCTAACGGAGGGGGCGATACTGAAGATCACGACGGAAGACCATGACCAGCAGTATAGGCCCATCCTGCAAGTGCACGACATGAAGCAGATCCACTCACAGGGCGGCGCCGGCGCGGACAAAGAGCGCTACAGGCTGGCCTTGTCCGATGGGTCCCACACCCAGCAGGGGATGCTTGCCACTCAGCAGAACTTCCTGATTCGGCAGGGCCACCTCCAGGTGGGCTCCGTCGTCTGCCTCAATCAATTCACCTGCACCACCGTCCAAGGCCGCCAGATCATCATTATAATCTCATTGGATGTGCTAGTTGATACATGTGAAATCATTGGAGAGCCTGCCAGAATTTCGAATGCCCAGTCCCCAGGGACAATACCTGGAAATGCTCAATCTCTTAACAGCAGTGCTCGTGTGGCGGGTCATGGTTCTTATTCTGGTAGTGCTGACCCAGGAAGATATGCTACAATGAGTACAGTTCCCAATCACCCTAGACCAGAGACTGGTTCTGGTTTTGCTGGGTTAGCACCAGTAAGTGGGTCATATGCTGGTCAGAACACAGGTTTACGCAGCCTCATATCCCAGGTTCCACCTCCACTTCGAAATTCTTATGCTCAGCCGACTTATCAACAGTCGCACTCAAATTCTTATGCCCGCCCGCCTCAGGCAACATATCAACAGCCACCTCCAAGTTCTTATGCACGCCCACCTCAGCCAACTCATCAACAGCCACCTCCATTATATAGTAACAGAGGACCAGTTGCCAAAAATGAAGCTCCTGGTGGAATAGTACAAATTTGTGCCCTGAATCGGTACCTAAATAAGTGGACAATTAAGGCTAGAGTGACGGCTAAATCAGAACTCAGGTCCTACACCAATGCACGTGGTGAGGGGAAAGTATTTAATTTTGATCTCATTGATTGTGGTGGGGAAATTCGTGTGACCTGCTTTAATCCAATAGCTGAAGAATTCTACAATTTGATTGAAGTTGGTAAGATGTACTTGATTTCTAAGGGTACAGTAAAGGCAGCTGTAAAGAAATTCAACCACCTTAGTAACGAGTTTGAAATTCAATTGGACACCACTTCGACAATACAGTTATGTTTTGAGGATGATGGCTCGATTCCACAGCAGCGGTTCAATTTTCGGCCAATAAGTGATGTTGAAGGTTTGGAGAACAACACTATTGTGGATATAATAGGTGTGGTAACATTCATCAGTCCTGCTGCTTCAATAAGCACGAAAAACGGTGAGACACAAAAGAGAACTCTCCAGTTGAAGGATATGTCTTGTCGTAGTGTTGAAGTAACTCTGTGGGGCAATTTGTGCAATGCAGAAGGGCAAAAACTTCAAAGTATGTGCGATTCTGGGTTATTTCCAGTGCTAGCTGTTAAAGCTGGTAGAGTCAATGACTTCAATGGCAAGGGGGTGGGGACCATGGCTACTAGTCAACTCTTTATAGAGCCCGATAACTCTCAAGCTGATGAGGTGAGGGAATGGTTTGACAGGGAAGGAAGGAATTCCCCATGTGTCTCTATCTCCAGGGGGACAGCAAGTGTGGGGAGAAATGATATCCGAAAGACTATATCCCAGATTAAAGATGAGAAGCTAGGGACTTCTGAGAAGCCAGATTGGATCACAATCAGTGCTACTGTCATATTTGTAAAGAGTGATAGCTACTTCTATGCAGCATGCCCACTCAAGATAGGGGAGCGGCAATGCAACAAAAAGGTTATAAATGATGGAGACGGGACATGGCGGTGTGAAAGATGTGAGCAGTCTGTAGAACAGTGTGACTACAGATATATACTCAACTTACAGATACAAGACCATACTGGCATAGCATGGGTAACTGCATTTCAGGAGGCTGGTGAAGAAATAATGGGTATAACTGCAAAAGATTTTCATAATTTGAAATATGAAATGCAGGATGAAGATAAAGTTGCAGAAATCGTTCGAAGGGTCTTGTTTACTGAATTTGTTTTCAAGTTAAAAATAAAGGAGGAGACTTTTAGTGATGAGCAGCGTGTGAAATCAACAATTGTCAAAGCAGATAAAGTTAGTTTCTCTTCAAAATCTAGATTTCATTTAGATCTGATGGATAGACTTAAAAATGGGGAAACTGCAATACCTTGTGCCAGGATGAACAATACTGAAGTCGGGAGTTCTGCAAGTAGACAACATACACCAGCTGTGAATGCTAGTTACGGTACCAATGCTGTCAATGCTGGTAGAGAGTTTGGAGCACCAGCAAATCAATTACATGTTGGAAGCCAGTATATTAGTCCTAGGGTTCCTTCAACTGGCTCTGCTGGTTTGTATCCTACCTGTAACATCTGTGGAGGTACAAGCCATAATTCTTTGAACTGCCCAAATGTTATGAATGGCCCAGGACAGTCATCAGGAGGGGGTTACACCAGTAGTAGGCCAGTTGCCAGTAATTCTGGTGAATGCTATAAATGTCACCAACCTGGGCACCGGGCGAAAGACTGTCCTAGCTCGAACCATATTCCAGCTTATGGAAGTAGTGGAGTCCAGCCGGGCAGATATGGAGTTTCAAATCAAAGGGTCAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

860

Amino Acids

94.09

Weight (kDa)

8.2

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 106 3.2e-25 Replication factor-A protein 1, N-terminal domain
tRNA_anti-codon PF01336 277 - 360 1.9e-09 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 386 - 488 1.8e-25 Replication protein A OB domain
Rep_fac-A_C PF08646 546 - 695 1.1e-49 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 296, 1739
AarI CACCTGC 1 cut(s) 269
Acc36I ACCTGC 2 cut(s) 269, 956
Acc65I GGTACC 2 cut(s) 816, 2213
AccB1I GGYRCC 4 cut(s) 116, 816, 2213, 2478
AccB7I CCANNNNNTGG 3 cut(s) 506, 539, 2522
AccBSI CCGCTC 1 cut(s) 1717
AccI GTMKAC 1 cut(s) 2179
AccII CGCG 1 cut(s) 125
AciI CCGC 9 cut(s) 114, 125, 280, 422, 658, 662, 1138, 1717, 1762
AclWI GGATC 2 cut(s) 94, 1646
AcoI YGGCCR 1 cut(s) 1151
AcuI CTGAAG 3 cut(s) 53, 987, 2178
AcvI CACGTG 1 cut(s) 887
AcyI GRCGYC 1 cut(s) 117
AdeI CACNNNGTG 1 cut(s) 890
AfaI GTAC 7 cut(s) 478, 795, 818, 1004, 1021, 2215, 2350
AfeI AGCGCT 1 cut(s) 137
AflIII ACRYGT 2 cut(s) 323, 2266
AhlI ACTAGT 1 cut(s) 1448
AjiI CACGTC 1 cut(s) 7
AleI CACNNNNGTG 1 cut(s) 1190
Alw21I GWGCWC 3 cut(s) 87, 415, 2251
Alw26I GTCTC 5 cut(s) 497, 1250, 1540, 1743, 2003
Alw44I GTGCAC 1 cut(s) 83
AlwI GGATC 2 cut(s) 94, 1646
AlwNI CAGNNNCTG 1 cut(s) 506
Aor51HI AGCGCT 1 cut(s) 137
AoxI GGCC 7 cut(s) 68, 147, 217, 277, 1151, 2389, 2429
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 6 cut(s) 573, 1031, 1135, 1229, 1691, 2026
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
Asp700I GAANNNNTTC 2 cut(s) 1522, 1956
Asp718I GGTACC 2 cut(s) 816, 2213
AspA2I CCTAGG 1 cut(s) 2291
AspLEI GCGC 3 cut(s) 119, 125, 138
AspS9I GGNCC 7 cut(s) 69, 162, 217, 758, 870, 1437, 2390
AsuC2I CCSGG 2 cut(s) 2483, 2544
AsuHPI GGTGA 8 cut(s) 250, 482, 902, 1265, 1504, 1886, 2459, 2462
AsuII TTCGAA 3 cut(s) 356, 604, 1960
AsuNHI GCTAGC 1 cut(s) 1387
AvaII GGWCC 4 cut(s) 162, 758, 870, 1437
AvrII CCTAGG 1 cut(s) 2291
AxyI CCTNAGG 1 cut(s) 666
BaeGI GKGCMC 3 cut(s) 87, 808, 2481
BanI GGYRCC 4 cut(s) 116, 816, 2213, 2478
BanII GRGCYC 2 cut(s) 236, 1470
BarI GAAGNNNNNNTAC 2 cut(s) 451, 483
BauI CACGAG 1 cut(s) 414
BbrPI CACGTG 1 cut(s) 887
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 3 cut(s) 87, 415, 2251
BbvCI CCTCAGC 1 cut(s) 714
BbvI GCAGC 6 cut(s) 585, 1043, 1147, 1216, 1703, 2038
BccI CCATC 5 cut(s) 80, 152, 1112, 1739, 2095
BceAI ACGGC 1 cut(s) 867
BciVI GTATCC 1 cut(s) 2334
BcnI CCSGG 2 cut(s) 2483, 2544
BcoDI GTCTC 5 cut(s) 497, 1250, 1540, 1743, 2003
BcuI ACTAGT 1 cut(s) 1448
BfmI CTRYAG 3 cut(s) 139, 1785, 1801
BfoI RGCGCY 2 cut(s) 120, 139
BfuAI ACCTGC 2 cut(s) 269, 956
BfuI GTATCC 1 cut(s) 2334
BglII AGATCT 1 cut(s) 2095
BisI GCNGC 9 cut(s) 115, 280, 574, 1032, 1136, 1230, 1692, 1718, 2027
BlnI CCTAGG 1 cut(s) 2291
BlpI GCTNAGC 1 cut(s) 618
BlsI GCNGC 9 cut(s) 116, 281, 575, 1033, 1137, 1231, 1693, 1719, 2028
Bme18I GGWCC 4 cut(s) 162, 758, 870, 1437
BmgBI CACGTC 1 cut(s) 7
BmgT120I GGNCC 7 cut(s) 69, 162, 217, 758, 870, 1437, 2390
BmrI ACTGGG 1 cut(s) 358
BmsI GCATC 1 cut(s) 171
BmtI GCTAGC 1 cut(s) 1391
BmuI ACTGGG 1 cut(s) 358
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 3 cut(s) 209, 1259, 1528
Bpu10I CCTNAGC 1 cut(s) 714
Bpu1102I GCTNAGC 1 cut(s) 618
Bpu14I TTCGAA 3 cut(s) 356, 604, 1960
BpuEI CTTGAG 2 cut(s) 1464, 1688
BpuMI CCSGG 2 cut(s) 2483, 2544
BsaAI YACGTR 1 cut(s) 887
BsaBI GATNNNNATC 1 cut(s) 1643
BsaHI GRCGYC 1 cut(s) 117
Bse118I RCCGGY 1 cut(s) 119
Bse21I CCTNAGG 1 cut(s) 666
Bse3DI GCAATG 2 cut(s) 1339, 1727
Bse8I GATNNNNATC 1 cut(s) 1643
BseGI GGATG 6 cut(s) 72, 186, 313, 1120, 1939, 2151
BseJI GATNNNNATC 1 cut(s) 1643
BseMI GCAATG 2 cut(s) 1339, 1727
BseMII CTCAG 6 cut(s) 206, 632, 680, 728, 880, 1614
BseRI GAGGAG 1 cut(s) 2021
BseSI GKGCMC 3 cut(s) 87, 808, 2481
BseXI GCAGC 6 cut(s) 585, 1043, 1147, 1216, 1703, 2038
BseYI CCCAGC 2 cut(s) 171, 521
BsgI GTGCAG 1 cut(s) 247
Bsh1236I CGCG 1 cut(s) 125
BshFI GGCC 7 cut(s) 70, 149, 219, 279, 1153, 2391, 2431
BshNI GGYRCC 4 cut(s) 116, 816, 2213, 2478
BsiHKAI GWGCWC 3 cut(s) 87, 415, 2251
BsiSI CCGG 3 cut(s) 120, 2482, 2543
BslFI GGGAC 7 cut(s) 148, 352, 388, 1450, 1564, 1630, 1768
BsmAI GTCTC 5 cut(s) 497, 1250, 1540, 1743, 2003
BsmBI CGTCTC 1 cut(s) 1743
BsmFI GGGAC 7 cut(s) 148, 352, 388, 1450, 1564, 1630, 1768
BsmI GAATGC 3 cut(s) 364, 2206, 2459
BsnI GGCC 7 cut(s) 70, 149, 219, 279, 1153, 2391, 2431
Bsp119I TTCGAA 3 cut(s) 356, 604, 1960
Bsp1286I GDGCHC 7 cut(s) 87, 236, 415, 808, 1470, 2251, 2481
Bsp143I GATC 6 cut(s) 36, 99, 285, 913, 1638, 2095
Bsp1720I GCTNAGC 1 cut(s) 618
Bsp19I CCATGG 1 cut(s) 1440
BspACI CCGC 9 cut(s) 114, 125, 280, 422, 658, 662, 1138, 1717, 1762
BspANI GGCC 7 cut(s) 70, 149, 219, 279, 1153, 2391, 2431
BspCNI CTCAG 6 cut(s) 205, 631, 679, 727, 879, 1615
BspFNI CGCG 1 cut(s) 125
BspMI ACCTGC 2 cut(s) 269, 956
BspOI GCTAGC 1 cut(s) 1391
BspPI GGATC 2 cut(s) 94, 1646
BspT104I TTCGAA 3 cut(s) 356, 604, 1960
BspT107I GGYRCC 4 cut(s) 116, 816, 2213, 2478
BsrBI CCGCTC 1 cut(s) 1717
BsrDI GCAATG 2 cut(s) 1339, 1727
BsrFI RCCGGY 1 cut(s) 119
BssAI RCCGGY 1 cut(s) 119
BssMI GATC 6 cut(s) 36, 99, 285, 913, 1638, 2095
BssNI GRCGYC 1 cut(s) 117
BssSI CACGAG 1 cut(s) 414
BssT1I CCWWGG 3 cut(s) 274, 1440, 2291
Bst2BI CACGAG 1 cut(s) 414
Bst6I CTCTTC 1 cut(s) 2077
BstACI GRCGYC 1 cut(s) 117
BstAPI GCANNNNNTGC 2 cut(s) 181, 1329
BstBAI YACGTR 1 cut(s) 887
BstBI TTCGAA 3 cut(s) 356, 604, 1960
BstC8I GCNNGC 9 cut(s) 121, 147, 186, 345, 658, 662, 706, 1389, 1696
BstDEI CTNAG 8 cut(s) 192, 618, 666, 714, 866, 1014, 1055, 1623
BstDSI CCRYGG 1 cut(s) 1440
BstF5I GGATG 6 cut(s) 72, 186, 313, 1120, 1939, 2151
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 2 cut(s) 120, 139
BstHHI GCGC 3 cut(s) 119, 125, 138
BstKTI GATC 6 cut(s) 39, 102, 288, 916, 1641, 2098
BstMAI GTCTC 5 cut(s) 497, 1250, 1540, 1743, 2003
BstMBI GATC 6 cut(s) 36, 99, 285, 913, 1638, 2095
BstNSI RCATGY 3 cut(s) 327, 1698, 2270
BstSFI CTRYAG 3 cut(s) 139, 1785, 1801
BstSLI GKGCMC 3 cut(s) 87, 808, 2481
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 6 cut(s) 585, 1043, 1147, 1216, 1703, 2038
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 2 cut(s) 99, 2095
BstYI RGATCY 2 cut(s) 99, 2095
Bsu36I CCTNAGG 1 cut(s) 666
BsuI GTATCC 1 cut(s) 2334
BsuRI GGCC 7 cut(s) 70, 149, 219, 279, 1153, 2391, 2431
BtgI CCRYGG 1 cut(s) 1440
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 6 cut(s) 72, 186, 313, 1120, 1939, 2151
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 4 cut(s) 415, 1389, 1654, 1799
BveI ACCTGC 2 cut(s) 269, 956
Cac8I GCNNGC 9 cut(s) 121, 147, 186, 345, 658, 662, 706, 1389, 1696
CaiI CAGNNNCTG 1 cut(s) 506
CfoI GCGC 3 cut(s) 119, 125, 138
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 7 cut(s) 69, 162, 217, 758, 870, 1437, 2390
Csp6I GTAC 7 cut(s) 477, 794, 817, 1003, 1020, 2214, 2349
CspCI CAANNNNNGTGG 2 cut(s) 868, 903
CviQI GTAC 7 cut(s) 477, 794, 817, 1003, 1020, 2214, 2349
DdeI CTNAG 8 cut(s) 192, 618, 666, 714, 866, 1014, 1055, 1623
DinI GGCGCC 1 cut(s) 118
DpnI GATC 6 cut(s) 38, 101, 287, 915, 1640, 2097
DpnII GATC 6 cut(s) 36, 99, 285, 913, 1638, 2095
DraIII CACNNNGTG 1 cut(s) 890
EaeI YGGCCR 1 cut(s) 1151
Eam1104I CTCTTC 1 cut(s) 2077
EarI CTCTTC 1 cut(s) 2077
Eco130I CCWWGG 3 cut(s) 274, 1440, 2291
Eco24I GRGCYC 2 cut(s) 236, 1470
Eco32I GATATC 1 cut(s) 1576
Eco47I GGWCC 4 cut(s) 162, 758, 870, 1437
Eco47III AGCGCT 1 cut(s) 137
Eco57I CTGAAG 3 cut(s) 53, 987, 2178
Eco72I CACGTG 1 cut(s) 887
Eco81I CCTNAGG 1 cut(s) 666
EcoO109I RGGNCCY 2 cut(s) 162, 870
EcoRI GAATTC 2 cut(s) 971, 1522
EcoRV GATATC 1 cut(s) 1576
EcoT14I CCWWGG 3 cut(s) 274, 1440, 2291
EcoT38I GRGCYC 2 cut(s) 236, 1470
EgeI GGCGCC 1 cut(s) 118
EheI GGCGCC 1 cut(s) 118
ErhI CCWWGG 3 cut(s) 274, 1440, 2291
Esp3I CGTCTC 1 cut(s) 1743
FalI AAGNNNNNCTT 2 cut(s) 989, 1021
FaqI GGGAC 7 cut(s) 148, 352, 388, 1450, 1564, 1630, 1768
FauI CCCGC 3 cut(s) 415, 665, 669
FauNDI CATATG 1 cut(s) 547
FblI GTMKAC 1 cut(s) 2179
Fnu4HI GCNGC 9 cut(s) 115, 280, 574, 1032, 1136, 1230, 1692, 1718, 2027
FokI GGATG 6 cut(s) 59, 193, 320, 1127, 1946, 2158
FriOI GRGCYC 2 cut(s) 236, 1470
Fsp4HI GCNGC 9 cut(s) 115, 280, 574, 1032, 1136, 1230, 1692, 1718, 2027
GlaI GCGC 3 cut(s) 118, 124, 137
GluI GCNGC 9 cut(s) 115, 280, 574, 1032, 1136, 1230, 1692, 1718, 2027
GsaI CCCAGC 2 cut(s) 175, 525
GsuI CTGGAG 3 cut(s) 209, 1259, 1528
HaeII RGCGCY 2 cut(s) 120, 139
HaeIII GGCC 7 cut(s) 70, 149, 219, 279, 1153, 2391, 2431
HapII CCGG 3 cut(s) 120, 2482, 2543
HhaI GCGC 3 cut(s) 119, 125, 138
Hin1I GRCGYC 1 cut(s) 117
Hin6I GCGC 3 cut(s) 117, 123, 136
HinP1I GCGC 3 cut(s) 117, 123, 136
HincII GTYRAC 1 cut(s) 1454
HindII GTYRAC 1 cut(s) 1454
HinfI GANTC 6 cut(s) 208, 811, 1126, 1367, 1407, 2535
HpaII CCGG 3 cut(s) 120, 2482, 2543
HphI GGTGA 8 cut(s) 250, 482, 902, 1265, 1504, 1886, 2459, 2462
Hpy166II GTNNAC 6 cut(s) 85, 569, 831, 1454, 1974, 2180
Hpy188I TCNGA 6 cut(s) 157, 558, 862, 1580, 1624, 2100
Hpy188III TCNNGA 7 cut(s) 40, 205, 1705, 1865, 2082, 2164, 2406
Hpy8I GTNNAC 6 cut(s) 85, 569, 831, 1454, 1974, 2180
Hpy99I CGWCG 2 cut(s) 46, 242
HpyAV CCTTC 7 cut(s) 1163, 1276, 1334, 1508, 1512, 1956, 2310
HpyCH4IV ACGT 2 cut(s) 6, 886
HpyF3I CTNAG 8 cut(s) 192, 618, 666, 714, 866, 1014, 1055, 1623
HpySE526I ACGT 2 cut(s) 6, 886
Hsp92I GRCGYC 1 cut(s) 117
HspAI GCGC 3 cut(s) 117, 123, 136
KasI GGCGCC 1 cut(s) 116
KflI GGGWCCC 1 cut(s) 162
KpnI GGTACC 2 cut(s) 820, 2217
KroI GCCGGC 1 cut(s) 119
KroNI GCCGGC 1 cut(s) 121
Kzo9I GATC 6 cut(s) 36, 99, 285, 913, 1638, 2095
LmnI GCTCC 4 cut(s) 239, 784, 1714, 2246
Lsp1109I GCAGC 6 cut(s) 585, 1043, 1147, 1216, 1703, 2038
LweI GCATC 1 cut(s) 171
MaeII ACGT 2 cut(s) 6, 886
MalI GATC 6 cut(s) 38, 101, 287, 915, 1640, 2097
MbiI CCGCTC 1 cut(s) 1717
MboI GATC 6 cut(s) 36, 99, 285, 913, 1638, 2095
MboII GAAGA 7 cut(s) 46, 59, 471, 980, 1889, 1949, 2064
MfeI CAATTG 2 cut(s) 1076, 2043
MflI RGATCY 2 cut(s) 99, 2095
MhlI GDGCHC 7 cut(s) 87, 236, 415, 808, 1470, 2251, 2481
Mly113I GGCGCC 1 cut(s) 117
MlyI GAGTC 2 cut(s) 1416, 2544
MmeI TCCRAC 1 cut(s) 2251
MreI CGCCGGCG 1 cut(s) 119
MroNI GCCGGC 1 cut(s) 119
MroXI GAANNNNTTC 2 cut(s) 1522, 1956
MseI TTAA 8 cut(s) 402, 837, 906, 954, 1395, 1599, 1994, 2112
MslI CAYNNNNRTG 2 cut(s) 1190, 1762
MspA1I CMGCKG 3 cut(s) 1034, 1138, 2195
MspI CCGG 3 cut(s) 120, 2482, 2543
MunI CAATTG 2 cut(s) 1076, 2043
Mva1269I GAATGC 3 cut(s) 364, 2206, 2459
MvnI CGCG 1 cut(s) 125
NaeI GCCGGC 1 cut(s) 121
NarI GGCGCC 1 cut(s) 117
NciI CCSGG 2 cut(s) 2483, 2544
NcoI CCATGG 1 cut(s) 1440
NdeI CATATG 1 cut(s) 547
NdeII GATC 6 cut(s) 36, 99, 285, 913, 1638, 2095
NgoMIV GCCGGC 1 cut(s) 119
NheI GCTAGC 1 cut(s) 1387
NmuCI GTSAC 4 cut(s) 847, 943, 1796, 2465
NspI RCATGY 3 cut(s) 327, 1698, 2270
NspV TTCGAA 3 cut(s) 356, 604, 1960
OliI CACNNNNGTG 1 cut(s) 1190
PaeI GCATGC 1 cut(s) 1698
PaqCI CACCTGC 1 cut(s) 269
PasI CCCWGGG 1 cut(s) 371
PciI ACATGT 2 cut(s) 323, 2266
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 3 cut(s) 364, 2206, 2459
PdiI GCCGGC 1 cut(s) 121
PdmI GAANNNNTTC 2 cut(s) 1522, 1956
PfeI GAWTC 4 cut(s) 208, 811, 1126, 1367
PflMI CCANNNNNTGG 3 cut(s) 506, 539, 2522
PkrI GCNGC 9 cut(s) 116, 281, 575, 1033, 1137, 1231, 1693, 1719, 2028
PleI GAGTC 2 cut(s) 1415, 2543
PluTI GGCGCC 1 cut(s) 120
PmaCI CACGTG 1 cut(s) 887
PmlI CACGTG 1 cut(s) 887
PpsI GAGTC 2 cut(s) 1415, 2543
Ppu21I YACGTR 1 cut(s) 887
PpuMI RGGWCCY 2 cut(s) 162, 870
PscI ACATGT 2 cut(s) 323, 2266
PsiI TTATAA 2 cut(s) 296, 1739
Psp5II RGGWCCY 2 cut(s) 162, 870
PspCI CACGTG 1 cut(s) 887
PspFI CCCAGC 2 cut(s) 171, 521
PspPI GGNCC 7 cut(s) 69, 162, 217, 758, 870, 1437, 2390
PspPPI RGGWCCY 2 cut(s) 162, 870
PstNI CAGNNNCTG 1 cut(s) 506
PsuI RGATCY 2 cut(s) 99, 2095
PvuII CAGCTG 2 cut(s) 1034, 2195
RsaI GTAC 7 cut(s) 478, 795, 818, 1004, 1021, 2215, 2350
RsaNI GTAC 7 cut(s) 477, 794, 817, 1003, 1020, 2214, 2349
RseI CAYNNNNRTG 2 cut(s) 1190, 1762
SaqAI TTAA 8 cut(s) 402, 837, 906, 954, 1395, 1599, 1994, 2112
SatI GCNGC 9 cut(s) 115, 280, 574, 1032, 1136, 1230, 1692, 1718, 2027
Sau3AI GATC 6 cut(s) 36, 99, 285, 913, 1638, 2095
Sau96I GGNCC 7 cut(s) 69, 162, 217, 758, 870, 1437, 2390
SchI GAGTC 2 cut(s) 1416, 2544
SduI GDGCHC 7 cut(s) 87, 236, 415, 808, 1470, 2251, 2481
SfaNI GCATC 1 cut(s) 171
SfcI CTRYAG 3 cut(s) 139, 1785, 1801
SfoI GGCGCC 1 cut(s) 118
SfuI TTCGAA 3 cut(s) 356, 604, 1960
SgrAI CRCCGGYG 1 cut(s) 119
SinI GGWCC 4 cut(s) 162, 758, 870, 1437
SmiMI CAYNNNNRTG 2 cut(s) 1190, 1762
SmlI CTYRAG 2 cut(s) 1479, 1703
SmoI CTYRAG 2 cut(s) 1479, 1703
SpeI ACTAGT 1 cut(s) 1448
SphI GCATGC 1 cut(s) 1698
SsiI CCGC 9 cut(s) 114, 125, 280, 422, 658, 662, 1138, 1717, 1762
SspDI GGCGCC 1 cut(s) 116
StyI CCWWGG 3 cut(s) 274, 1440, 2291
TaiI ACGT 2 cut(s) 9, 889
TaqI TCGA 6 cut(s) 356, 604, 1091, 1124, 1960, 2504
TatI WGTACW 3 cut(s) 476, 793, 1002
TauI GCSGC 3 cut(s) 117, 282, 1720
TfiI GAWTC 4 cut(s) 208, 811, 1126, 1367
Tru1I TTAA 8 cut(s) 402, 837, 906, 954, 1395, 1599, 1994, 2112
Tru9I TTAA 8 cut(s) 402, 837, 906, 954, 1395, 1599, 1994, 2112
TscAI CASTG 4 cut(s) 415, 1389, 1654, 1799
TseFI GTSAC 4 cut(s) 847, 943, 1796, 2465
TseI GCWGC 6 cut(s) 573, 1031, 1135, 1229, 1691, 2026
Tsp45I GTSAC 4 cut(s) 847, 943, 1796, 2465
TspDTI ATGAA 9 cut(s) 107, 789, 1207, 1898, 1938, 1950, 2078, 2162, 2399
TspGWI ACGGA 3 cut(s) 32, 59, 226
TspRI CASTG 4 cut(s) 415, 1389, 1654, 1799
Van91I CCANNNNNTGG 3 cut(s) 506, 539, 2522
VneI GTGCAC 1 cut(s) 83
VpaK11BI GGWCC 4 cut(s) 162, 758, 870, 1437
XbaI TCTAGA 1 cut(s) 2081
XceI RCATGY 3 cut(s) 327, 1698, 2270
XmaJI CCTAGG 1 cut(s) 2291
XmiI GTMKAC 1 cut(s) 2179
XmnI GAANNNNTTC 2 cut(s) 1522, 1956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.