RLG00000002810

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009053
Physical Location & Seq
Forward (+)
40207046 .. 40210148
3103 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002810

Sequence Viewer

Length: 2655 bp
ATGGACGTGAGGCTAACGGAGGGGGCGATACTGAAGATCATGACGGAAGACCATGACAAGCAGTATAGGCCCATCCTGCAAGTGCTCGACATCAAGCAGATCCACGCACACCGCGGCGGCGGCGGCGGCGGCCCGAACACCGAGCGCTACAGGCTGGCCTTGTTCGATGGGTCCCACACGCAGCAGGGGATGCTTGCCACTCAGCAGAACTTCCTGATCCAGCAGGGCCACCTCCAGAAGGGCTCCGTCGTCTGCCTCAACCAATTCACCTGCACCACCGTCCAAGGCCGCCAGATCATCATTATAGTCGCATTGGATGTGCTAGTTGATACATGTGAAATCATTGGACAGCCTGCCAGAATTTCAAATGCACAGTCCCCAGGGACAATACCTGGAAATGCCCAATCTCTTAACAGCAGTGCTCGTGTGGCGGGTCATGAAACTGTGACTGAGGCGACTCCCCAACAACCTAGATTGAATCAATCGTATGGTGGTTCTTATTCTGGTAGTGGTGACCCAGGAAGATACGCTACGATGAGTACAGTTCCCAATCACCCTAGACCAGAGACTGGTTCTGGTTTTGCTGGGTTAGCACCAGGAAGTGGGTCATATGCTGGTCAGAACACAGGTTTACGCAGCCCCATATCCCAGGTTCCATGTCCACTTCAAAATTCTTATGCTCACCCACCTCAGCCGACTTATCAACAGTCACACTCAAATTCTTATGCCGACCCGCCTCAGGCAACTTATCAACAGCCACCTCCAAGTTCTTATGCACGCCCACCTCAGCCAACTCATCAACAGCCACCTCCATTATATAGTAACAGAGGACCAGTTGCCAAAAATGAAGCTCCTGGTAGAATAGTACAAATTTGTGCCCTGAATCGGTACCTAAATAAGTGGACAATTAAGGCTAGAGTGACGGCTAAATCAGAACTCAGGTCCTACACCAATGCACGTGGTGAGGGGAAAGTATTTAATTTTGATCTTATTGATTGTGGTGGGGAAATTCGTGTGACCTGCTTTAATCCAATAGCTGAAGAATTCTACAATTTGATTGAAGTTGGTAAGGTGTACTTGATTTCTAAGGGTACAGTAAAGGCAGCTGTAAAGAAATTCAACCACCTTGATAACGAGTTTGAAATTCAATTGGACACCACTTCGACAATACAGTTATGTTTTGAGGATGATGGCTTGATTCCACAGCAGCGGTTCAATTTTCGGCCAATAAGTGATGTTGAAGGCTTGGAGAACAACATTATTGTGGATATAATAGGTGTGGTAACATTCATCAGTCCTGCTGCTTCAATAAGCACGAAAAACGGTGAGACACAAAAGAGAACTCTCCAGTTGAAGGATATGTCTTGTCGTAGCGTTGAAGTAACTCTGTGGGGCAATTTGTGTAATGTAGAAGGGCAAAAACTTCAAAGTATGTGCGATTCTGGGTTATTTCCAGTGCTAGCTGTTAAAGCTGGTAGAGTCAATGACTTCAATGGCAAGGGGGTGGGGACCATGGCTACTAGTCAACTCTTTATAGAGCCCAATAACTCTCAAGCTGATGAGGTGAGGGAATGGTTTGACAGGGAAGGAAGGAATTCCCCATGTGTCTCTATCTCCAGGGGAACAGCAAGTGTGGGGAGAAATGATATCCGAAAGACTATATCCCAGATTAAAGATGAGAAGCTAGGGACTTCTGAGAAGCCAGATTGGATCACAATCAGTGCTACTGTCATATTTATAAAGAGTGATAACTACTTCTATGCAGCATGCCCACTCAAGATAGGGGAGCGGCAATGCAACAAAAAGGTTATAAATGATGGAGACGGGACATGGCGGTGTGAAAGATGTGAGCAGTCCGTAGAACAGTGTGACTACAGATATATACTCAACTTACAGATACAAGACCATACTGGCATAGCATGGGTAACTGCATTTCAGGAGGCTGGTGAAGAAATAATGGGTATAACTGCAAAAGATTTTCATAATTTGAAATATGAAATGCAGGATGAAGATAAAGTTGCAGAAATCATTCGAAGGGTCTTGTTTACTGAATTCGTTTTCAAGTTGAAAATAAAGGAGGAGACTTTTAGTGATGAGCAGCGTGTGAAATCAACAATTGTCAAAGCAGATAAAGTTAGTTTCTCTTCAAAATCTAGATTTCATTTAGATCTGATGGATAGACTTAAAAATGGGGAAACTGCAATACCTTGTGCCGGGATGAACAATACTGAAGTGGGGAGTTCTGCAATTAGACAACATACACCAGCTGTGAATGCTAGTTACAGTACCAATGCTGTCAATGCTGGTAGAGAGTTTGGAGCACCAGCAAATCAAGTACATGTTGGAAGCCAGTATATTAGTCCTAGGGTTCCTACAACTGGCTCTGCTGGTTTGTATCCTACCTGTAACATCTGTGGAGGCACAAGCCATAATTCTTTGAACTGCCCAAATGTTATGAATGGCCCAGGACAGTCATCAGGAGGGGGTTACACCAATAGTAGGCCAGTTGCCAGTAATTCTGGTGAATGCTATAAATGTCACCAACCTGGGCACTGGGCGAAAGACTGTCCTAGCTCGAACCATATTCTAGCTTATGGAAGTAGTGGAGTCCAGCCAGGCAGATATGGAGTTTCAAATCAAAGGGTCAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

885

Amino Acids

96.8

Weight (kDa)

7.91

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 109 1.8e-24 Replication factor-A protein 1, N-terminal domain
tRNA_anti-codon PF01336 301 - 384 4.2e-10 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 410 - 512 7.1e-25 Replication protein A OB domain
Rep_fac-A_C PF08646 570 - 719 1.9e-50 Replication factor-A C terminal domain
zf-CCHC PF00098 842 - 858 6.7e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1739, 1811
AarI CACCTGC 1 cut(s) 278
Acc36I ACCTGC 2 cut(s) 278, 1028
Acc65I GGTACC 1 cut(s) 888
AccB1I GGYRCC 1 cut(s) 888
AccB7I CCANNNNNTGG 2 cut(s) 569, 602
AccBSI CCGCTC 1 cut(s) 1789
AccII CGCG 1 cut(s) 114
AclWI GGATC 3 cut(s) 94, 211, 1718
AcoI YGGCCR 1 cut(s) 1223
AcuI CTGAAG 3 cut(s) 53, 1059, 2250
AcvI CACGTG 1 cut(s) 959
AdeI CACNNNGTG 1 cut(s) 962
AfaI GTAC 7 cut(s) 541, 867, 890, 1076, 1093, 2287, 2337
AfeI AGCGCT 1 cut(s) 146
AfiI CCNNNNNNNGG 9 cut(s) 238, 569, 602, 739, 885, 1354, 2213, 2378, 2499
AflIII ACRYGT 2 cut(s) 332, 2338
AhlI ACTAGT 1 cut(s) 1520
AjiI CACGTC 1 cut(s) 7
Alw21I GWGCWC 3 cut(s) 87, 424, 2323
Alw26I GTCTC 5 cut(s) 560, 1322, 1612, 1815, 2075
AlwI GGATC 3 cut(s) 94, 211, 1718
AlwNI CAGNNNCTG 1 cut(s) 569
Aor51HI AGCGCT 1 cut(s) 146
AoxI GGCC 8 cut(s) 68, 130, 156, 226, 286, 1223, 2461, 2501
ApeKI GCWGC 7 cut(s) 181, 636, 1103, 1207, 1301, 1763, 2098
ArsI GACNNNNNNTTYG 2 cut(s) 359, 391
Asp700I GAANNNNTTC 2 cut(s) 1594, 2028
Asp718I GGTACC 1 cut(s) 888
AspA2I CCTAGG 1 cut(s) 2363
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 8 cut(s) 69, 131, 171, 226, 830, 942, 1509, 2462
AsuC2I CCSGG 1 cut(s) 2215
AsuII TTCGAA 1 cut(s) 2032
AsuNHI GCTAGC 1 cut(s) 1459
AvaII GGWCC 4 cut(s) 171, 830, 942, 1509
AvrII CCTAGG 1 cut(s) 2363
AxyI CCTNAGG 1 cut(s) 738
BaeGI GKGCMC 2 cut(s) 880, 2553
BanI GGYRCC 1 cut(s) 888
BanII GRGCYC 2 cut(s) 245, 1542
BarI GAAGNNNNNNTAC 2 cut(s) 514, 546
BauI CACGAG 1 cut(s) 423
BbrPI CACGTG 1 cut(s) 959
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 3 cut(s) 87, 424, 2323
BbvCI CCTCAGC 2 cut(s) 690, 786
BbvI GCAGC 7 cut(s) 193, 648, 1115, 1219, 1288, 1775, 2110
BccI CCATC 5 cut(s) 80, 161, 1184, 1811, 2167
BceAI ACGGC 1 cut(s) 939
BciVI GTATCC 1 cut(s) 2406
BcnI CCSGG 1 cut(s) 2215
BcoDI GTCTC 5 cut(s) 560, 1322, 1612, 1815, 2075
BcuI ACTAGT 1 cut(s) 1520
BfmI CTRYAG 2 cut(s) 148, 1873
BfoI RGCGCY 1 cut(s) 148
BfuAI ACCTGC 2 cut(s) 278, 1028
BfuI GTATCC 1 cut(s) 2406
BglII AGATCT 1 cut(s) 2167
BlnI CCTAGG 1 cut(s) 2363
Bme18I GGWCC 4 cut(s) 171, 830, 942, 1509
BmgBI CACGTC 1 cut(s) 7
BmgT120I GGNCC 8 cut(s) 69, 131, 171, 226, 830, 942, 1509, 2462
BmiI GGNNCC 7 cut(s) 172, 173, 244, 654, 890, 1510, 2370
BmrI ACTGGG 1 cut(s) 2563
BmsI GCATC 1 cut(s) 180
BmtI GCTAGC 1 cut(s) 1463
BmuI ACTGGG 1 cut(s) 2563
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 3 cut(s) 218, 1331, 1600
Bpu10I CCTNAGC 2 cut(s) 690, 786
Bpu14I TTCGAA 1 cut(s) 2032
BpuEI CTTGAG 2 cut(s) 1536, 1760
BpuMI CCSGG 1 cut(s) 2215
BsaAI YACGTR 1 cut(s) 959
BsaBI GATNNNNATC 1 cut(s) 1715
Bsc4I CCNNNNNNNGG 9 cut(s) 238, 569, 602, 739, 885, 1354, 2213, 2378, 2499
Bse21I CCTNAGG 1 cut(s) 738
Bse3DI GCAATG 1 cut(s) 1799
Bse8I GATNNNNATC 1 cut(s) 1715
BseGI GGATG 6 cut(s) 72, 195, 322, 1192, 2011, 2223
BseJI GATNNNNATC 1 cut(s) 1715
BseLI CCNNNNNNNGG 9 cut(s) 238, 569, 602, 739, 885, 1354, 2213, 2378, 2499
BseMI GCAATG 1 cut(s) 1799
BseMII CTCAG 7 cut(s) 215, 441, 704, 752, 800, 952, 1686
BseRI GAGGAG 1 cut(s) 2093
BseSI GKGCMC 2 cut(s) 880, 2553
BseXI GCAGC 7 cut(s) 193, 648, 1115, 1219, 1288, 1775, 2110
BseYI CCCAGC 1 cut(s) 584
BsgI GTGCAG 1 cut(s) 256
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 8 cut(s) 70, 132, 158, 228, 288, 1225, 2463, 2503
BshNI GGYRCC 1 cut(s) 888
BsiHKAI GWGCWC 3 cut(s) 87, 424, 2323
BsiSI CCGG 1 cut(s) 2214
BslFI GGGAC 6 cut(s) 157, 361, 397, 1522, 1702, 1840
BslI CCNNNNNNNGG 9 cut(s) 238, 569, 602, 739, 885, 1354, 2213, 2378, 2499
BsmAI GTCTC 5 cut(s) 560, 1322, 1612, 1815, 2075
BsmBI CGTCTC 1 cut(s) 1815
BsmFI GGGAC 6 cut(s) 157, 361, 397, 1522, 1702, 1840
BsmI GAATGC 2 cut(s) 2278, 2531
BsnI GGCC 8 cut(s) 70, 132, 158, 228, 288, 1225, 2463, 2503
Bsp119I TTCGAA 1 cut(s) 2032
Bsp1286I GDGCHC 7 cut(s) 87, 245, 424, 880, 1542, 2323, 2553
Bsp143I GATC 7 cut(s) 36, 99, 216, 294, 985, 1710, 2167
Bsp19I CCATGG 1 cut(s) 1512
BspANI GGCC 8 cut(s) 70, 132, 158, 228, 288, 1225, 2463, 2503
BspCNI CTCAG 7 cut(s) 214, 442, 703, 751, 799, 951, 1687
BspFNI CGCG 1 cut(s) 114
BspHI TCATGA 2 cut(s) 39, 436
BspLI GGNNCC 7 cut(s) 172, 173, 244, 654, 890, 1510, 2370
BspMI ACCTGC 2 cut(s) 278, 1028
BspOI GCTAGC 1 cut(s) 1463
BspPI GGATC 3 cut(s) 94, 211, 1718
BspT104I TTCGAA 1 cut(s) 2032
BspT107I GGYRCC 1 cut(s) 888
BsrBI CCGCTC 1 cut(s) 1789
BsrDI GCAATG 1 cut(s) 1799
BssMI GATC 7 cut(s) 36, 99, 216, 294, 985, 1710, 2167
BssSI CACGAG 1 cut(s) 423
BssT1I CCWWGG 3 cut(s) 283, 1512, 2363
Bst2BI CACGAG 1 cut(s) 423
Bst6I CTCTTC 1 cut(s) 2149
BstAPI GCANNNNNTGC 1 cut(s) 190
BstBAI YACGTR 1 cut(s) 959
BstBI TTCGAA 1 cut(s) 2032
BstC8I GCNNGC 6 cut(s) 156, 195, 354, 778, 1461, 1768
BstDEI CTNAG 8 cut(s) 201, 450, 690, 738, 786, 938, 1086, 1695
BstDSI CCRYGG 2 cut(s) 112, 1512
BstEII GGTNACC 1 cut(s) 512
BstENI CCTNNNNNAGG 1 cut(s) 236
BstF5I GGATG 6 cut(s) 72, 195, 322, 1192, 2011, 2223
BstFNI CGCG 1 cut(s) 114
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 7 cut(s) 39, 102, 219, 297, 988, 1713, 2170
BstMAI GTCTC 5 cut(s) 560, 1322, 1612, 1815, 2075
BstMBI GATC 7 cut(s) 36, 99, 216, 294, 985, 1710, 2167
BstNSI RCATGY 3 cut(s) 336, 1770, 2342
BstPI GGTNACC 1 cut(s) 512
BstSFI CTRYAG 2 cut(s) 148, 1873
BstSLI GKGCMC 2 cut(s) 880, 2553
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 7 cut(s) 193, 648, 1115, 1219, 1288, 1775, 2110
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 2 cut(s) 99, 2167
BstYI RGATCY 2 cut(s) 99, 2167
Bsu36I CCTNAGG 1 cut(s) 738
BsuI GTATCC 1 cut(s) 2406
BsuRI GGCC 8 cut(s) 70, 132, 158, 228, 288, 1225, 2463, 2503
BtgI CCRYGG 2 cut(s) 112, 1512
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 6 cut(s) 72, 195, 322, 1192, 2011, 2223
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 5 cut(s) 424, 1461, 1726, 1871, 2551
BveI ACCTGC 2 cut(s) 278, 1028
Cac8I GCNNGC 6 cut(s) 156, 195, 354, 778, 1461, 1768
CaiI CAGNNNCTG 1 cut(s) 569
CciI TCATGA 2 cut(s) 39, 436
CfoI GCGC 1 cut(s) 147
Cfr13I GGNCC 8 cut(s) 69, 131, 171, 226, 830, 942, 1509, 2462
Cfr42I CCGCGG 1 cut(s) 115
Csp6I GTAC 7 cut(s) 540, 866, 889, 1075, 1092, 2286, 2336
CspCI CAANNNNNGTGG 2 cut(s) 940, 975
CviQI GTAC 7 cut(s) 540, 866, 889, 1075, 1092, 2286, 2336
DdeI CTNAG 8 cut(s) 201, 450, 690, 738, 786, 938, 1086, 1695
DpnI GATC 7 cut(s) 38, 101, 218, 296, 987, 1712, 2169
DpnII GATC 7 cut(s) 36, 99, 216, 294, 985, 1710, 2167
DraIII CACNNNGTG 1 cut(s) 962
EaeI YGGCCR 1 cut(s) 1223
Eam1104I CTCTTC 1 cut(s) 2149
EarI CTCTTC 1 cut(s) 2149
Eco130I CCWWGG 3 cut(s) 283, 1512, 2363
Eco24I GRGCYC 2 cut(s) 245, 1542
Eco32I GATATC 1 cut(s) 1648
Eco47I GGWCC 4 cut(s) 171, 830, 942, 1509
Eco47III AGCGCT 1 cut(s) 146
Eco57I CTGAAG 3 cut(s) 53, 1059, 2250
Eco72I CACGTG 1 cut(s) 959
Eco81I CCTNAGG 1 cut(s) 738
Eco91I GGTNACC 1 cut(s) 512
EcoNI CCTNNNNNAGG 1 cut(s) 236
EcoO109I RGGNCCY 2 cut(s) 171, 942
EcoO65I GGTNACC 1 cut(s) 512
EcoRI GAATTC 3 cut(s) 1043, 1594, 2051
EcoRV GATATC 1 cut(s) 1648
EcoT14I CCWWGG 3 cut(s) 283, 1512, 2363
EcoT38I GRGCYC 2 cut(s) 245, 1542
ErhI CCWWGG 3 cut(s) 283, 1512, 2363
Esp3I CGTCTC 1 cut(s) 1815
FalI AAGNNNNNCTT 2 cut(s) 1061, 1093
FaqI GGGAC 6 cut(s) 157, 361, 397, 1522, 1702, 1840
FauI CCCGC 2 cut(s) 424, 741
FauNDI CATATG 1 cut(s) 610
FokI GGATG 6 cut(s) 59, 202, 329, 1199, 2018, 2230
FriOI GRGCYC 2 cut(s) 245, 1542
GlaI GCGC 1 cut(s) 146
GsaI CCCAGC 1 cut(s) 588
GsuI CTGGAG 3 cut(s) 218, 1331, 1600
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 8 cut(s) 70, 132, 158, 228, 288, 1225, 2463, 2503
HapII CCGG 1 cut(s) 2214
HhaI GCGC 1 cut(s) 147
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HincII GTYRAC 1 cut(s) 1526
HindII GTYRAC 1 cut(s) 1526
HinfI GANTC 7 cut(s) 457, 478, 883, 1198, 1439, 1479, 2607
HpaII CCGG 1 cut(s) 2214
Hpy166II GTNNAC 6 cut(s) 632, 662, 903, 1075, 1526, 2046
Hpy188I TCNGA 5 cut(s) 621, 934, 1652, 1696, 2172
Hpy188III TCNNGA 8 cut(s) 40, 214, 235, 437, 1777, 1937, 2154, 2478
Hpy8I GTNNAC 6 cut(s) 632, 662, 903, 1075, 1526, 2046
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 7 cut(s) 232, 1235, 1348, 1406, 1580, 1584, 2028
HpyCH4IV ACGT 2 cut(s) 6, 958
HpyF3I CTNAG 8 cut(s) 201, 450, 690, 738, 786, 938, 1086, 1695
HpySE526I ACGT 2 cut(s) 6, 958
HspAI GCGC 1 cut(s) 145
KflI GGGWCCC 1 cut(s) 171
KpnI GGTACC 1 cut(s) 892
KspI CCGCGG 1 cut(s) 115
Kzo9I GATC 7 cut(s) 36, 99, 216, 294, 985, 1710, 2167
LmnI GCTCC 4 cut(s) 248, 856, 1786, 2318
Lsp1109I GCAGC 7 cut(s) 193, 648, 1115, 1219, 1288, 1775, 2110
LweI GCATC 1 cut(s) 180
MaeII ACGT 2 cut(s) 6, 958
MalI GATC 7 cut(s) 38, 101, 218, 296, 987, 1712, 2169
MbiI CCGCTC 1 cut(s) 1789
MboI GATC 7 cut(s) 36, 99, 216, 294, 985, 1710, 2167
MboII GAAGA 7 cut(s) 46, 59, 534, 1052, 1961, 2021, 2136
MfeI CAATTG 2 cut(s) 1148, 2115
MflI RGATCY 2 cut(s) 99, 2167
MhlI GDGCHC 7 cut(s) 87, 245, 424, 880, 1542, 2323, 2553
MlyI GAGTC 3 cut(s) 451, 1488, 2616
MmeI TCCRAC 1 cut(s) 2323
MroXI GAANNNNTTC 2 cut(s) 1594, 2028
MseI TTAA 7 cut(s) 411, 909, 978, 1026, 1467, 1671, 2184
MslI CAYNNNNRTG 2 cut(s) 1262, 1834
MspA1I CMGCKG 4 cut(s) 114, 1106, 1210, 2267
MspI CCGG 1 cut(s) 2214
MunI CAATTG 2 cut(s) 1148, 2115
Mva1269I GAATGC 2 cut(s) 2278, 2531
MvnI CGCG 1 cut(s) 114
NciI CCSGG 1 cut(s) 2215
NcoI CCATGG 1 cut(s) 1512
NdeI CATATG 1 cut(s) 610
NdeII GATC 7 cut(s) 36, 99, 216, 294, 985, 1710, 2167
NheI GCTAGC 1 cut(s) 1459
NlaIV GGNNCC 7 cut(s) 172, 173, 244, 654, 890, 1510, 2370
NmuCI GTSAC 7 cut(s) 445, 512, 708, 919, 1015, 1868, 2537
NspI RCATGY 3 cut(s) 336, 1770, 2342
NspV TTCGAA 1 cut(s) 2032
PaeI GCATGC 1 cut(s) 1770
PagI TCATGA 2 cut(s) 39, 436
PaqCI CACCTGC 1 cut(s) 278
PasI CCCWGGG 1 cut(s) 380
PciI ACATGT 2 cut(s) 332, 2338
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 2 cut(s) 2278, 2531
PdmI GAANNNNTTC 2 cut(s) 1594, 2028
PfeI GAWTC 4 cut(s) 478, 883, 1198, 1439
PflMI CCANNNNNTGG 2 cut(s) 569, 602
PleI GAGTC 3 cut(s) 451, 1487, 2615
PmaCI CACGTG 1 cut(s) 959
PmlI CACGTG 1 cut(s) 959
PpsI GAGTC 3 cut(s) 451, 1487, 2615
Ppu21I YACGTR 1 cut(s) 959
PpuMI RGGWCCY 2 cut(s) 171, 942
PscI ACATGT 2 cut(s) 332, 2338
PsiI TTATAA 2 cut(s) 1739, 1811
Psp5II RGGWCCY 2 cut(s) 171, 942
PspCI CACGTG 1 cut(s) 959
PspEI GGTNACC 1 cut(s) 512
PspFI CCCAGC 1 cut(s) 584
PspN4I GGNNCC 7 cut(s) 172, 173, 244, 654, 890, 1510, 2370
PspPI GGNCC 8 cut(s) 69, 131, 171, 226, 830, 942, 1509, 2462
PspPPI RGGWCCY 2 cut(s) 171, 942
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 2 cut(s) 99, 2167
PvuII CAGCTG 2 cut(s) 1106, 2267
RsaI GTAC 7 cut(s) 541, 867, 890, 1076, 1093, 2287, 2337
RsaNI GTAC 7 cut(s) 540, 866, 889, 1075, 1092, 2286, 2336
RseI CAYNNNNRTG 2 cut(s) 1262, 1834
SacII CCGCGG 1 cut(s) 115
SaqAI TTAA 7 cut(s) 411, 909, 978, 1026, 1467, 1671, 2184
Sau3AI GATC 7 cut(s) 36, 99, 216, 294, 985, 1710, 2167
Sau96I GGNCC 8 cut(s) 69, 131, 171, 226, 830, 942, 1509, 2462
SchI GAGTC 3 cut(s) 451, 1488, 2616
SduI GDGCHC 7 cut(s) 87, 245, 424, 880, 1542, 2323, 2553
SfaNI GCATC 1 cut(s) 180
SfcI CTRYAG 2 cut(s) 148, 1873
Sfr303I CCGCGG 1 cut(s) 115
SfuI TTCGAA 1 cut(s) 2032
SgrBI CCGCGG 1 cut(s) 115
SinI GGWCC 4 cut(s) 171, 830, 942, 1509
SmiMI CAYNNNNRTG 2 cut(s) 1262, 1834
SmlI CTYRAG 2 cut(s) 1551, 1775
SmoI CTYRAG 2 cut(s) 1551, 1775
SpeI ACTAGT 1 cut(s) 1520
SphI GCATGC 1 cut(s) 1770
StyI CCWWGG 3 cut(s) 283, 1512, 2363
TaiI ACGT 2 cut(s) 9, 961
TaqI TCGA 5 cut(s) 87, 165, 1163, 2032, 2576
TatI WGTACW 4 cut(s) 539, 865, 1074, 2335
TauI GCSGC 8 cut(s) 117, 120, 123, 126, 129, 132, 291, 1792
TfiI GAWTC 4 cut(s) 478, 883, 1198, 1439
Tru1I TTAA 7 cut(s) 411, 909, 978, 1026, 1467, 1671, 2184
Tru9I TTAA 7 cut(s) 411, 909, 978, 1026, 1467, 1671, 2184
TscAI CASTG 5 cut(s) 424, 1461, 1726, 1871, 2558
TseFI GTSAC 7 cut(s) 445, 512, 708, 919, 1015, 1868, 2537
TseI GCWGC 7 cut(s) 181, 636, 1103, 1207, 1301, 1763, 2098
Tsp45I GTSAC 7 cut(s) 445, 512, 708, 919, 1015, 1868, 2537
TspDTI ATGAA 9 cut(s) 453, 861, 1279, 1970, 2010, 2022, 2150, 2234, 2471
TspGWI ACGGA 4 cut(s) 32, 59, 235, 1846
TspRI CASTG 5 cut(s) 424, 1461, 1726, 1871, 2558
Van91I CCANNNNNTGG 2 cut(s) 569, 602
VpaK11BI GGWCC 4 cut(s) 171, 830, 942, 1509
XagI CCTNNNNNAGG 1 cut(s) 236
XbaI TCTAGA 1 cut(s) 2153
XceI RCATGY 3 cut(s) 336, 1770, 2342
XmaJI CCTAGG 1 cut(s) 2363
XmnI GAANNNNTTC 2 cut(s) 1594, 2028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.