Rroxscaffold_3G00246040

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009053
Physical Location & Seq
Forward (+)
39127809 .. 39131133
3325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00246040.1

Sequence Viewer

Length: 2556 bp
ATGGACGTGAGGCTAACGGAGGGGGCGATACTGAAGATGACGACGGGGGACTATGTCGAGCCGTATAGGCCCATCCTGCAAGTGTACGACATGAAGCAGATCCACGCACAGGGCGGCGCGGTCGCCGAGCGGTACAGGCTAGCCTTGTCCGATGGGTCCCGCACCCAGCAGGGGATGCTTGCCACTCAGCAGAACTTCCTGATCCAGCAGGGCCACCTCCAGACCGGCTCCGTCGTCTGCCTCAACCAATTCACCTGCGCCCCCATCCGAGGCCGCCAGATCATCATTATAGTCTCATTGGATGTGCTAGTTGCTACATGTGAAATCATTGGACAGCCTGCCAGAATTTCGAATGCACAGTCCCCAGGGACAATACCTGGAAATGCCCAATCTCTTAACAGCAGTGCTAGTGTGGCGGGTCATGAAACTGTGACTGAGGCGACTCCCCAACATCCTAGATTGAATCAATCGTATGGTGGTTCTTATTCTGGTAATGCTGACCCAGGAAGATGTGCTACAATGAGTACAGTTCCCAATCACCCTAGACCAGAGACTGGTTCTGGTTTTGCTGGGTTAGCACCAGTAAGTGGGTCATTTGCTGGTCAGAACACAGGTTTAAGCAGCCCCATATCCCAGGTTCCACCTCCACTTCAAAATTCTTATGCTCACCCACCTCAGCCGGCTTATCAACAGTCGCACTCAAATTCTTATGCCCGCCCGCCTCAGGCAACTTATCAACAGCCACCTCCAAGTTCTTATGCACGCCCACCTCTGCCAGCTCATCAACAGCCACCTCCATTATATAGTAACAGAGGACCAGTTGCCAAAAATGAAGCTCCTGGTAGAATAGTACAAATTTGTGCCCTGAATCCGTACCAAAATAGATGGACAATTAAGGCTAGAGTGGCGGCTAAATCAGAACTCAGGTCCTACACCAATGCACGTGGTGAGGGGAAAGTTTTTAATTTTGATCTTATTGATTCTACTGGGGAAATTCGTGTAACCTGCTTTAATCCAGTAGCCGAAGAATTCTACAATTTGATTGAAGTTGGTAAGGTGTACCTGATCTCAAAGGGTACTGTGAAGAGAGCTGTAAGGAAATTCAACCACCTCAGCAACGAGTTTGAAATTCAATTGGACACCACTTCGACAATACAATTATGTTTTGAGGATGATGGCTCAATTCCACAGCAGCGGTTCAATTTTCGGCCAATAAGTGATGTTGAAGGCTTGGAGAACAACATTATTGTGGATATAATAGGTGTGGTAACATTCATCAGTCCTGCTGCTTCAATAAGCACGAAAAACGGTGAGACACAAAAGAGAACTCTCCAGTTGAAGGATATGTCTTGTCGTAGCGTTGAAGTAACTCTGTGGGGCAATTTGTGCAATGTAGAAGGGCAAAAACTTCAAAGTATGTGCGATTCTGGATTATTTCCAGTGCTAGCTGTTAAAGCTGAGCCCAATAACTCTCAAGCTGATGAGGTGAGGGAATGGTTTGACAGGGAAGGAAGGAATTCCCCATGTGTCTCTATCTCCAGGGGAACAGCAAGTGTGGGGAGAAATGATATCCGAAAGACTATATCCCAGATTAAAGATGAGAAGCTAGGGACTTCTGAGAAGCCAGATTGGATCACAATCAGTGCTACTGTCACATTTATAAAGACATGCCCACTCAAGATAGGGGAGCGGCAATGCAACAAAAAGGTTATAAATGATGGAGACGGGACATGGCGGTGTGAAAGATGTGAGCAGTCTGTACAACAGTGTGACTACAGATATATACTCAACTTACAGATACAAGACCATACTGGCATAGCATGGGTAACTGCATTTCAGGAGGCTGGTGAAGAAATAATGGGTATTACTGCAAAAGATTTTCATAATTTGAAATATGAAATGCAGGATGAAGATAAAGTTGCAGAAATCATTCGAAGGGTCTTGTTTACTGAATTCGTTTTCAAGTTGAAAATAAAGGAGGAGACTTTTAGTGATGAGCAGCGTGTGAAATCAACAATTGTCAAAGCAGATAAAGTTAGTTTCTCTTCAAAATCTAGATTTCATTTAGATCTGATGGATAGACTTAAAAATGGGGAAACTGCAATACCTTGTGCCGGGATGAACAATACTGAAGTGGGGAGTTCTGCAATTAGACAACATACACCAGCTGTGAATGCTAGTTACAGTACCAATGCTGTCACTGCTGGTAGAGAGTTTGGAGCACCAGCAAATCAAGTACATGTTGGAAGCCAGTGTATTAGTCCTAGGGGTCCTTCAACTGGCTCTGCTGGTTTGTATCCTACCTGTAACATCTGTGGAGGTACAAGCCATAATTCTTTGAACTGCCCAAATGTTATGAATGGCCCAGGACAGTCATCAGGAGGGGGTTACACCAATAGTAGGCCAGTTGCCAATAATTCTGGTGAATGCTATAAATGTCTCCAACCTGGGCACTGGGCGAAAGACTGTCCTAGCTCGAACCATCTTCCAGCTTATGGAAGTAGTGGAGTCCAGCCGGGCAGATATGGAGTTTCAAATCAAAGGGTCAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

851

Amino Acids

92.9

Weight (kDa)

8.29

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 104 7.6e-24 Replication factor-A protein 1, N-terminal domain
tRNA_anti-codon PF01336 296 - 379 2.3e-11 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 405 - 486 1.4e-16 Replication protein A OB domain
CDC24_OB3 PF17244 417 - 635 5.3e-07 Cell division control protein 24, OB domain 3
Rep_fac-A_C PF08646 544 - 686 2.6e-43 Replication factor-A C terminal domain
zf-CCHC PF00098 809 - 825 2.9e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1661, 1712
AarI CACCTGC 1 cut(s) 263
Acc36I ACCTGC 2 cut(s) 263, 1013
AccB7I CCANNNNNTGG 3 cut(s) 554, 587, 2495
AccBSI CCGCTC 2 cut(s) 130, 1690
AccII CGCG 1 cut(s) 119
AclWI GGATC 3 cut(s) 94, 196, 1640
AcoI YGGCCR 1 cut(s) 1208
AcuI CTGAAG 2 cut(s) 53, 2151
AcvI CACGTG 1 cut(s) 944
AdeI CACNNNGTG 1 cut(s) 947
AflIII ACRYGT 2 cut(s) 317, 2239
AjiI CACGTC 1 cut(s) 7
AjnI CCWGG 8 cut(s) 364, 376, 502, 633, 838, 1538, 2365, 2446
Alw21I GWGCWC 1 cut(s) 2224
Alw26I GTCTC 7 cut(s) 298, 545, 1307, 1534, 1716, 1976, 2444
AlwI GGATC 3 cut(s) 94, 196, 1640
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 6 cut(s) 68, 211, 271, 1208, 2362, 2402
ApeKI GCWGC 4 cut(s) 621, 1192, 1286, 1999
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
Asp700I GAANNNNTTC 2 cut(s) 1516, 1929
AspA2I CCTAGG 1 cut(s) 2264
AspLEI GCGC 2 cut(s) 119, 260
AspS9I GGNCC 7 cut(s) 69, 156, 211, 815, 927, 2270, 2363
AsuC2I CCSGG 2 cut(s) 2116, 2517
AsuHPI GGTGA 8 cut(s) 244, 530, 659, 959, 1322, 1498, 1859, 2435
AsuII TTCGAA 2 cut(s) 350, 1933
AsuNHI GCTAGC 2 cut(s) 139, 1444
AvaII GGWCC 4 cut(s) 156, 815, 927, 2270
AvrII CCTAGG 1 cut(s) 2264
AxyI CCTNAGG 1 cut(s) 723
BaeGI GKGCMC 2 cut(s) 865, 2454
BanII GRGCYC 1 cut(s) 1464
BarI GAAGNNNNNNTAC 2 cut(s) 499, 531
BbrPI CACGTG 1 cut(s) 944
Bbv12I GWGCWC 1 cut(s) 2224
BbvCI CCTCAGC 2 cut(s) 675, 1112
BbvI GCAGC 4 cut(s) 633, 1204, 1273, 2011
BccI CCATC 8 cut(s) 80, 146, 272, 879, 1169, 1712, 2068, 2490
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 8 cut(s) 366, 378, 504, 635, 840, 1540, 2367, 2448
BciVI GTATCC 1 cut(s) 2307
BcnI CCSGG 2 cut(s) 2116, 2517
BcoDI GTCTC 7 cut(s) 298, 545, 1307, 1534, 1716, 1976, 2444
BfmI CTRYAG 1 cut(s) 1774
BfuAI ACCTGC 2 cut(s) 263, 1013
BfuI GTATCC 1 cut(s) 2307
BglI GCCNNNNNGGC 1 cut(s) 67
BglII AGATCT 1 cut(s) 2068
BisI GCNGC 8 cut(s) 115, 274, 622, 909, 1193, 1287, 1691, 2000
BlnI CCTAGG 1 cut(s) 2264
BlpI GCTNAGC 1 cut(s) 1458
BlsI GCNGC 8 cut(s) 116, 275, 623, 910, 1194, 1288, 1692, 2001
Bme18I GGWCC 4 cut(s) 156, 815, 927, 2270
BmgBI CACGTC 1 cut(s) 7
BmgT120I GGNCC 7 cut(s) 69, 156, 211, 815, 927, 2270, 2363
BmiI GGNNCC 5 cut(s) 157, 158, 229, 639, 2271
BmrI ACTGGG 2 cut(s) 996, 2464
BmsI GCATC 1 cut(s) 165
BmtI GCTAGC 2 cut(s) 143, 1448
BmuI ACTGGG 2 cut(s) 996, 2464
BpmI CTGGAG 3 cut(s) 203, 1316, 1522
Bpu10I CCTNAGC 2 cut(s) 675, 1112
Bpu1102I GCTNAGC 1 cut(s) 1458
Bpu14I TTCGAA 2 cut(s) 350, 1933
BpuEI CTTGAG 2 cut(s) 1458, 1661
BpuMI CCSGG 2 cut(s) 2116, 2517
BsaAI YACGTR 1 cut(s) 944
BsaBI GATNNNNATC 1 cut(s) 1637
BsaJI CCNNGG 9 cut(s) 268, 364, 365, 502, 633, 1539, 2264, 2365, 2447
Bse118I RCCGGY 2 cut(s) 224, 679
Bse21I CCTNAGG 1 cut(s) 723
Bse3DI GCAATG 2 cut(s) 1396, 1700
Bse8I GATNNNNATC 1 cut(s) 1637
BseBI CCWGG 8 cut(s) 366, 378, 504, 635, 840, 1540, 2367, 2448
BseDI CCNNGG 9 cut(s) 268, 364, 365, 502, 633, 1539, 2264, 2365, 2447
BseGI GGATG 8 cut(s) 72, 180, 264, 307, 451, 1177, 1912, 2124
BseJI GATNNNNATC 1 cut(s) 1637
BseMI GCAATG 2 cut(s) 1396, 1700
BseMII CTCAG 8 cut(s) 200, 426, 689, 737, 937, 1126, 1449, 1608
BseRI GAGGAG 1 cut(s) 1994
BseSI GKGCMC 2 cut(s) 865, 2454
BseXI GCAGC 4 cut(s) 633, 1204, 1273, 2011
BseYI CCCAGC 2 cut(s) 165, 569
Bsh1236I CGCG 1 cut(s) 119
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 6 cut(s) 70, 213, 273, 1210, 2364, 2404
BsiEI CGRYCG 1 cut(s) 123
BsiHKAI GWGCWC 1 cut(s) 2224
BsiSI CCGG 4 cut(s) 225, 680, 2115, 2516
BslFI GGGAC 6 cut(s) 62, 142, 346, 382, 1624, 1741
BsmAI GTCTC 7 cut(s) 298, 545, 1307, 1534, 1716, 1976, 2444
BsmBI CGTCTC 1 cut(s) 1716
BsmFI GGGAC 6 cut(s) 62, 142, 346, 382, 1624, 1741
BsmI GAATGC 3 cut(s) 358, 2179, 2432
BsnI GGCC 6 cut(s) 70, 213, 273, 1210, 2364, 2404
Bsp119I TTCGAA 2 cut(s) 350, 1933
Bsp1286I GDGCHC 4 cut(s) 865, 1464, 2224, 2454
Bsp1407I TGTACA 1 cut(s) 1759
Bsp143I GATC 7 cut(s) 99, 201, 279, 970, 1065, 1632, 2068
Bsp1720I GCTNAGC 1 cut(s) 1458
BspANI GGCC 6 cut(s) 70, 213, 273, 1210, 2364, 2404
BspCNI CTCAG 8 cut(s) 199, 427, 688, 736, 936, 1125, 1450, 1609
BspFNI CGCG 1 cut(s) 119
BspHI TCATGA 1 cut(s) 421
BspLI GGNNCC 5 cut(s) 157, 158, 229, 639, 2271
BspMI ACCTGC 2 cut(s) 263, 1013
BspOI GCTAGC 2 cut(s) 143, 1448
BspPI GGATC 3 cut(s) 94, 196, 1640
BspT104I TTCGAA 2 cut(s) 350, 1933
BsrBI CCGCTC 2 cut(s) 130, 1690
BsrDI GCAATG 2 cut(s) 1396, 1700
BsrFI RCCGGY 2 cut(s) 224, 679
BsrGI TGTACA 1 cut(s) 1759
BssAI RCCGGY 2 cut(s) 224, 679
BssECI CCNNGG 9 cut(s) 268, 364, 365, 502, 633, 1539, 2264, 2365, 2447
BssMI GATC 7 cut(s) 99, 201, 279, 970, 1065, 1632, 2068
BssT1I CCWWGG 1 cut(s) 2264
Bst2UI CCWGG 8 cut(s) 366, 378, 504, 635, 840, 1540, 2367, 2448
Bst6I CTCTTC 2 cut(s) 1079, 2050
BstAPI GCANNNNNTGC 2 cut(s) 175, 1386
BstAUI TGTACA 1 cut(s) 1759
BstBAI YACGTR 1 cut(s) 944
BstBI TTCGAA 2 cut(s) 350, 1933
BstC8I GCNNGC 9 cut(s) 141, 180, 339, 681, 715, 719, 763, 777, 1446
BstDEI CTNAG 8 cut(s) 186, 435, 675, 723, 923, 1112, 1458, 1617
BstF5I GGATG 8 cut(s) 72, 180, 264, 307, 451, 1177, 1912, 2124
BstFNI CGCG 1 cut(s) 119
BstHHI GCGC 2 cut(s) 119, 260
BstKTI GATC 7 cut(s) 102, 204, 282, 973, 1068, 1635, 2071
BstMAI GTCTC 7 cut(s) 298, 545, 1307, 1534, 1716, 1976, 2444
BstMBI GATC 7 cut(s) 99, 201, 279, 970, 1065, 1632, 2068
BstMCI CGRYCG 1 cut(s) 123
BstNI CCWGG 8 cut(s) 366, 378, 504, 635, 840, 1540, 2367, 2448
BstNSI RCATGY 3 cut(s) 321, 1671, 2243
BstSFI CTRYAG 1 cut(s) 1774
BstSLI GKGCMC 2 cut(s) 865, 2454
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 4 cut(s) 633, 1204, 1273, 2011
BstX2I RGATCY 2 cut(s) 99, 2068
BstYI RGATCY 2 cut(s) 99, 2068
Bsu36I CCTNAGG 1 cut(s) 723
BsuI GTATCC 1 cut(s) 2307
BsuRI GGCC 6 cut(s) 70, 213, 273, 1210, 2364, 2404
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 8 cut(s) 72, 180, 264, 307, 451, 1177, 1912, 2124
BtsI GCAGTG 2 cut(s) 409, 2199
BtsIMutI CAGTG 7 cut(s) 409, 1446, 1648, 1772, 2199, 2258, 2452
BveI ACCTGC 2 cut(s) 263, 1013
Cac8I GCNNGC 9 cut(s) 141, 180, 339, 681, 715, 719, 763, 777, 1446
CaiI CAGNNNCTG 1 cut(s) 554
CciI TCATGA 1 cut(s) 421
CfoI GCGC 2 cut(s) 119, 260
Cfr10I RCCGGY 2 cut(s) 224, 679
Cfr13I GGNCC 7 cut(s) 69, 156, 211, 815, 927, 2270, 2363
CspCI CAANNNNNGTGG 2 cut(s) 925, 960
CviAII CATG 8 cut(s) 91, 318, 422, 1524, 1668, 1731, 1821, 2240
DdeI CTNAG 8 cut(s) 186, 435, 675, 723, 923, 1112, 1458, 1617
DpnI GATC 7 cut(s) 101, 203, 281, 972, 1067, 1634, 2070
DpnII GATC 7 cut(s) 99, 201, 279, 970, 1065, 1632, 2068
DraIII CACNNNGTG 1 cut(s) 947
EaeI YGGCCR 1 cut(s) 1208
Eam1104I CTCTTC 2 cut(s) 1079, 2050
EarI CTCTTC 2 cut(s) 1079, 2050
Eco130I CCWWGG 1 cut(s) 2264
Eco24I GRGCYC 1 cut(s) 1464
Eco32I GATATC 1 cut(s) 1570
Eco47I GGWCC 4 cut(s) 156, 815, 927, 2270
Eco57I CTGAAG 2 cut(s) 53, 2151
Eco72I CACGTG 1 cut(s) 944
Eco81I CCTNAGG 1 cut(s) 723
EcoO109I RGGNCCY 3 cut(s) 156, 927, 2270
EcoRI GAATTC 3 cut(s) 1028, 1516, 1952
EcoRII CCWGG 8 cut(s) 364, 376, 502, 633, 838, 1538, 2365, 2446
EcoRV GATATC 1 cut(s) 1570
EcoT14I CCWWGG 1 cut(s) 2264
EcoT38I GRGCYC 1 cut(s) 1464
ErhI CCWWGG 1 cut(s) 2264
Esp3I CGTCTC 1 cut(s) 1716
FaeI CATG 8 cut(s) 94, 321, 425, 1527, 1671, 1734, 1824, 2243
FaqI GGGAC 6 cut(s) 62, 142, 346, 382, 1624, 1741
FatI CATG 8 cut(s) 90, 317, 421, 1523, 1667, 1730, 1820, 2239
FauI CCCGC 4 cut(s) 167, 409, 722, 726
Fnu4HI GCNGC 8 cut(s) 115, 274, 622, 909, 1193, 1287, 1691, 2000
FokI GGATG 8 cut(s) 59, 187, 251, 314, 438, 1184, 1919, 2131
FriOI GRGCYC 1 cut(s) 1464
Fsp4HI GCNGC 8 cut(s) 115, 274, 622, 909, 1193, 1287, 1691, 2000
GlaI GCGC 2 cut(s) 118, 259
GluI GCNGC 8 cut(s) 115, 274, 622, 909, 1193, 1287, 1691, 2000
GsaI CCCAGC 2 cut(s) 169, 573
GsuI CTGGAG 3 cut(s) 203, 1316, 1522
HaeIII GGCC 6 cut(s) 70, 213, 273, 1210, 2364, 2404
HapII CCGG 4 cut(s) 225, 680, 2115, 2516
HhaI GCGC 2 cut(s) 119, 260
Hin1II CATG 8 cut(s) 94, 321, 425, 1527, 1671, 1734, 1824, 2243
Hin6I GCGC 2 cut(s) 117, 258
HinP1I GCGC 2 cut(s) 117, 258
HinfI GANTC 6 cut(s) 442, 463, 868, 980, 1424, 2508
HpaII CCGG 4 cut(s) 225, 680, 2115, 2516
HphI GGTGA 8 cut(s) 244, 530, 659, 959, 1322, 1498, 1859, 2435
Hpy166II GTNNAC 3 cut(s) 85, 1060, 1947
Hpy188I TCNGA 7 cut(s) 151, 269, 606, 919, 1574, 1618, 2073
Hpy188III TCNNGA 8 cut(s) 199, 220, 422, 1428, 1678, 1838, 2055, 2379
Hpy8I GTNNAC 3 cut(s) 85, 1060, 1947
Hpy99I CGWCG 2 cut(s) 46, 236
HpyAV CCTTC 7 cut(s) 1220, 1333, 1391, 1502, 1506, 1929, 2283
HpyCH4IV ACGT 2 cut(s) 6, 943
HpyF3I CTNAG 8 cut(s) 186, 435, 675, 723, 923, 1112, 1458, 1617
HpySE526I ACGT 2 cut(s) 6, 943
Hsp92II CATG 8 cut(s) 94, 321, 425, 1527, 1671, 1734, 1824, 2243
HspAI GCGC 2 cut(s) 117, 258
KflI GGGWCCC 1 cut(s) 156
KroI GCCGGC 1 cut(s) 679
KroNI GCCGGC 1 cut(s) 681
Kzo9I GATC 7 cut(s) 99, 201, 279, 970, 1065, 1632, 2068
LmnI GCTCC 4 cut(s) 233, 841, 1687, 2219
Lsp1109I GCAGC 4 cut(s) 633, 1204, 1273, 2011
LweI GCATC 1 cut(s) 165
MaeII ACGT 2 cut(s) 6, 943
MalI GATC 7 cut(s) 101, 203, 281, 972, 1067, 1634, 2070
MbiI CCGCTC 2 cut(s) 130, 1690
MboI GATC 7 cut(s) 99, 201, 279, 970, 1065, 1632, 2068
MboII GAAGA 8 cut(s) 46, 519, 1037, 1096, 1862, 1922, 2037, 2477
MfeI CAATTG 2 cut(s) 1133, 2016
MflI RGATCY 2 cut(s) 99, 2068
MhlI GDGCHC 4 cut(s) 865, 1464, 2224, 2454
MlyI GAGTC 2 cut(s) 436, 2517
MmeI TCCRAC 2 cut(s) 2224, 2467
MroNI GCCGGC 1 cut(s) 679
MroXI GAANNNNTTC 2 cut(s) 1516, 1929
MseI TTAA 8 cut(s) 396, 617, 894, 963, 1011, 1452, 1593, 2085
MslI CAYNNNNRTG 2 cut(s) 1247, 1735
MspA1I CMGCKG 2 cut(s) 1195, 2168
MspI CCGG 4 cut(s) 225, 680, 2115, 2516
MunI CAATTG 2 cut(s) 1133, 2016
Mva1269I GAATGC 3 cut(s) 358, 2179, 2432
MvaI CCWGG 8 cut(s) 366, 378, 504, 635, 840, 1540, 2367, 2448
MvnI CGCG 1 cut(s) 119
NaeI GCCGGC 1 cut(s) 681
NciI CCSGG 2 cut(s) 2116, 2517
NdeII GATC 7 cut(s) 99, 201, 279, 970, 1065, 1632, 2068
NgoMIV GCCGGC 1 cut(s) 679
NheI GCTAGC 2 cut(s) 139, 1444
NlaIII CATG 8 cut(s) 94, 321, 425, 1527, 1671, 1734, 1824, 2243
NlaIV GGNNCC 5 cut(s) 157, 158, 229, 639, 2271
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 4 cut(s) 430, 1651, 1769, 2197
NspI RCATGY 3 cut(s) 321, 1671, 2243
NspV TTCGAA 2 cut(s) 350, 1933
PagI TCATGA 1 cut(s) 421
PaqCI CACCTGC 1 cut(s) 263
PasI CCCWGGG 1 cut(s) 365
PciI ACATGT 2 cut(s) 317, 2239
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 3 cut(s) 358, 2179, 2432
PdiI GCCGGC 1 cut(s) 681
PdmI GAANNNNTTC 2 cut(s) 1516, 1929
PfeI GAWTC 4 cut(s) 463, 868, 980, 1424
PflFI GACNNNGTC 1 cut(s) 53
PflMI CCANNNNNTGG 3 cut(s) 554, 587, 2495
PkrI GCNGC 8 cut(s) 116, 275, 623, 910, 1194, 1288, 1692, 2001
PleI GAGTC 2 cut(s) 436, 2516
PmaCI CACGTG 1 cut(s) 944
PmlI CACGTG 1 cut(s) 944
PpsI GAGTC 2 cut(s) 436, 2516
Ppu21I YACGTR 1 cut(s) 944
PpuMI RGGWCCY 3 cut(s) 156, 927, 2270
PscI ACATGT 2 cut(s) 317, 2239
PsiI TTATAA 2 cut(s) 1661, 1712
Psp5II RGGWCCY 3 cut(s) 156, 927, 2270
Psp6I CCWGG 8 cut(s) 364, 376, 502, 633, 838, 1538, 2365, 2446
PspCI CACGTG 1 cut(s) 944
PspFI CCCAGC 2 cut(s) 165, 569
PspGI CCWGG 8 cut(s) 364, 376, 502, 633, 838, 1538, 2365, 2446
PspN4I GGNNCC 5 cut(s) 157, 158, 229, 639, 2271
PspPI GGNCC 7 cut(s) 69, 156, 211, 815, 927, 2270, 2363
PspPPI RGGWCCY 3 cut(s) 156, 927, 2270
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 2 cut(s) 99, 2068
PsyI GACNNNGTC 1 cut(s) 53
PvuII CAGCTG 1 cut(s) 2168
RseI CAYNNNNRTG 2 cut(s) 1247, 1735
SaqAI TTAA 8 cut(s) 396, 617, 894, 963, 1011, 1452, 1593, 2085
SatI GCNGC 8 cut(s) 115, 274, 622, 909, 1193, 1287, 1691, 2000
Sau3AI GATC 7 cut(s) 99, 201, 279, 970, 1065, 1632, 2068
Sau96I GGNCC 7 cut(s) 69, 156, 211, 815, 927, 2270, 2363
SchI GAGTC 2 cut(s) 436, 2517
SduI GDGCHC 4 cut(s) 865, 1464, 2224, 2454
SfaNI GCATC 1 cut(s) 165
SfcI CTRYAG 1 cut(s) 1774
SfuI TTCGAA 2 cut(s) 350, 1933
SinI GGWCC 4 cut(s) 156, 815, 927, 2270
SmiMI CAYNNNNRTG 2 cut(s) 1247, 1735
SmlI CTYRAG 2 cut(s) 1473, 1676
SmoI CTYRAG 2 cut(s) 1473, 1676
StyI CCWWGG 1 cut(s) 2264
TaiI ACGT 2 cut(s) 9, 946
TaqI TCGA 5 cut(s) 57, 350, 1148, 1933, 2477
TatI WGTACW 4 cut(s) 524, 850, 1759, 2236
TauI GCSGC 4 cut(s) 117, 276, 911, 1693
TfiI GAWTC 4 cut(s) 463, 868, 980, 1424
Tru1I TTAA 8 cut(s) 396, 617, 894, 963, 1011, 1452, 1593, 2085
Tru9I TTAA 8 cut(s) 396, 617, 894, 963, 1011, 1452, 1593, 2085
TscAI CASTG 7 cut(s) 409, 1446, 1648, 1772, 2206, 2258, 2459
TseFI GTSAC 4 cut(s) 430, 1651, 1769, 2197
TseI GCWGC 4 cut(s) 621, 1192, 1286, 1999
Tsp45I GTSAC 4 cut(s) 430, 1651, 1769, 2197
TspGWI ACGGA 3 cut(s) 32, 220, 861
TspRI CASTG 7 cut(s) 409, 1446, 1648, 1772, 2206, 2258, 2459
Tth111I GACNNNGTC 1 cut(s) 53
Van91I CCANNNNNTGG 3 cut(s) 554, 587, 2495
VpaK11BI GGWCC 4 cut(s) 156, 815, 927, 2270
XbaI TCTAGA 1 cut(s) 2054
XceI RCATGY 3 cut(s) 321, 1671, 2243
XmaJI CCTAGG 1 cut(s) 2264
XmnI GAANNNNTTC 2 cut(s) 1516, 1929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.