AT5G46060

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004799
Physical Location & Seq
Forward (+)
18681326 .. 18682708
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46060.1

Sequence Viewer

Length: 747 bp
ATGGAGATATTTGTGAAGAAAGAGTACTTAGATTTGGTTCTTGTCCCATTTGGATTAATCATAGTTTTATCTTACCATCTATTTCTTCTCTATAGAATCCTTTACTTTCCTTACCATACCATTATCGGTTTCATGAACATCGATAAATCCATTTGGGTCGACCGTATCATGCAGGCAAGAAAAGACGAATTGGGTGGTGCTTTAACTGTTCTTTCGAGCAGCATATCAGCTTCAACATTTATGGCATCCATTGCTTTAACATTGAGTTCACTTATCGGCGCGTGGATCGGAAGCTCGCCGGTTAGTATGACTGTTTTTACCGGACATTTTATCTATGGAGACACGAGTTCAATCACAATGGTCATCAAATACACATCGCTCCTCATATGTTTCCTAGTTGCATTCTGCTGTTTTATTCAATCCACGAGATGTTTTCTACATGCGAACTATCTTATCACCACTCCGGGCGAAGATATTCCCCCGGACATGGTGAAGAGATTTGTCTTGAGAGGCGGGAACTATTGGTCGCTAGGTCTTAGGGCTCTTTATTTGGCTCTTGATCTATTGTTGTGGCTTTTCGGACCGGTTCCGATGTTTATTAACTCGGTTTTGATGGTCATTTGTTTGTATTTTCTCGACTCAAACTCGGTGGCTCAGCCTCTTTATCACCGGACTTTTGAGGCGGAGCAAATTGTCAAGAAGATGAGAGGAGTTTTGCCTGAACAGTTGACTTTTAGATGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

248

Amino Acids

28.17

Weight (kDa)

8.18

Isoelectric Point (pI)

32.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 13 - 216 8.3e-62 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 159
AccII CGCG 1 cut(s) 281
AciI CCGC 2 cut(s) 513, 683
AclWI GGATC 1 cut(s) 293
AfaI GTAC 1 cut(s) 26
AfiI CCNNNNNNNGG 1 cut(s) 487
AgeI ACCGGT 1 cut(s) 583
AgsI TTSAA 3 cut(s) 234, 351, 419
AluBI AGCT 2 cut(s) 230, 294
AluI AGCT 2 cut(s) 230, 294
Alw26I GTCTC 1 cut(s) 333
AlwI GGATC 1 cut(s) 293
ApeKI GCWGC 1 cut(s) 219
AseI ATTAAT 1 cut(s) 56
AsiGI ACCGGT 1 cut(s) 583
Asp700I GAANNNNTTC 1 cut(s) 474
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 1 cut(s) 581
AsuC2I CCSGG 2 cut(s) 465, 482
AsuHPI GGTGA 3 cut(s) 448, 502, 659
AvaII GGWCC 1 cut(s) 581
BanII GRGCYC 1 cut(s) 544
BarI GAAGNNNNNNTAC 2 cut(s) 8, 40
BauI CACGAG 2 cut(s) 343, 424
BbvI GCAGC 1 cut(s) 231
BccI CCATC 2 cut(s) 84, 607
BcnI CCSGG 2 cut(s) 465, 482
BcoDI GTCTC 1 cut(s) 333
BfaI CTAG 2 cut(s) 395, 530
BfmI CTRYAG 1 cut(s) 91
BisI GCNGC 1 cut(s) 220
BlpI GCTNAGC 1 cut(s) 654
BlsI GCNGC 1 cut(s) 221
BmcAI AGTACT 1 cut(s) 26
Bme1390I CCNGG 2 cut(s) 465, 482
Bme18I GGWCC 1 cut(s) 581
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 1 cut(s) 588
BmrFI CCNGG 2 cut(s) 465, 482
BmsI GCATC 1 cut(s) 254
Bpu1102I GCTNAGC 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 526
BpuMI CCSGG 2 cut(s) 465, 482
Bsa29I ATCGAT 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 480
BsaWI WCCGGW 3 cut(s) 320, 583, 669
Bsc4I CCNNNNNNNGG 1 cut(s) 487
Bse118I RCCGGY 2 cut(s) 298, 583
Bse3DI GCAATG 1 cut(s) 249
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 480
BseGI GGATG 1 cut(s) 245
BseLI CCNNNNNNNGG 1 cut(s) 487
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 1 cut(s) 668
BseRI GAGGAG 2 cut(s) 371, 723
BseXI GCAGC 1 cut(s) 231
Bsh1236I CGCG 1 cut(s) 281
Bsh1285I CGRYCG 1 cut(s) 163
BshTI ACCGGT 1 cut(s) 583
BshVI ATCGAT 1 cut(s) 141
BsiEI CGRYCG 1 cut(s) 163
BsiSI CCGG 6 cut(s) 299, 321, 464, 482, 584, 670
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 1 cut(s) 487
BsmAI GTCTC 1 cut(s) 333
BsmFI GGGAC 1 cut(s) 29
BsmI GAATGC 1 cut(s) 401
Bsp1286I GDGCHC 1 cut(s) 544
Bsp143I GATC 2 cut(s) 285, 559
Bsp1720I GCTNAGC 1 cut(s) 654
BspACI CCGC 2 cut(s) 513, 683
BspCNI CTCAG 1 cut(s) 667
BspDI ATCGAT 1 cut(s) 141
BspFNI CGCG 1 cut(s) 281
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 588
BspPI GGATC 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 249
BsrFI RCCGGY 2 cut(s) 298, 583
BssAI RCCGGY 2 cut(s) 298, 583
BssECI CCNNGG 1 cut(s) 480
BssMI GATC 2 cut(s) 285, 559
BssSI CACGAG 2 cut(s) 343, 424
Bst2BI CACGAG 2 cut(s) 343, 424
Bst4CI ACNGT 4 cut(s) 164, 208, 313, 726
Bst6I CTCTTC 1 cut(s) 488
BstAPI GCANNNNNTGC 1 cut(s) 251
BstC8I GCNNGC 2 cut(s) 174, 296
BstDEI CTNAG 3 cut(s) 28, 536, 654
BstF5I GGATG 1 cut(s) 245
BstFNI CGCG 1 cut(s) 281
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 2 cut(s) 288, 562
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 2 cut(s) 285, 559
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNSI RCATGY 1 cut(s) 443
BstSCI CCNGG 2 cut(s) 463, 480
BstSFI CTRYAG 1 cut(s) 91
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 141
BsuTUI ATCGAT 1 cut(s) 141
BtgZI GCGATG 1 cut(s) 360
BtsCI GGATG 1 cut(s) 245
Cac8I GCNNGC 2 cut(s) 174, 296
CciI TCATGA 1 cut(s) 132
CfoI GCGC 1 cut(s) 281
Cfr10I RCCGGY 2 cut(s) 298, 583
Cfr13I GGNCC 1 cut(s) 581
ClaI ATCGAT 1 cut(s) 141
CpoI CGGWCCG 1 cut(s) 581
Csp6I GTAC 1 cut(s) 25
CspAI ACCGGT 1 cut(s) 583
CspCI CAANNNNNGTGG 2 cut(s) 630, 665
CspI CGGWCCG 1 cut(s) 581
CviAII CATG 4 cut(s) 133, 169, 440, 487
CviJI RGCY 7 cut(s) 230, 294, 542, 554, 574, 653, 658
CviKI_1 RGCY 7 cut(s) 230, 294, 542, 554, 574, 653, 658
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 3 cut(s) 28, 536, 654
DpnI GATC 2 cut(s) 287, 561
DpnII GATC 2 cut(s) 285, 559
Eam1104I CTCTTC 1 cut(s) 488
EarI CTCTTC 1 cut(s) 488
EciI GGCGGA 1 cut(s) 698
Eco24I GRGCYC 1 cut(s) 544
Eco47I GGWCC 1 cut(s) 581
EcoT38I GRGCYC 1 cut(s) 544
FaeI CATG 4 cut(s) 136, 172, 443, 490
FaqI GGGAC 1 cut(s) 29
FatI CATG 4 cut(s) 132, 168, 439, 486
FauI CCCGC 1 cut(s) 506
FauNDI CATATG 1 cut(s) 386
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 220
FokI GGATG 1 cut(s) 232
FriOI GRGCYC 1 cut(s) 544
Fsp4HI GCNGC 1 cut(s) 220
FspBI CTAG 2 cut(s) 395, 530
GlaI GCGC 1 cut(s) 280
GluI GCNGC 1 cut(s) 220
HapII CCGG 6 cut(s) 299, 321, 464, 482, 584, 670
HhaI GCGC 1 cut(s) 281
Hin1II CATG 4 cut(s) 136, 172, 443, 490
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HincII GTYRAC 2 cut(s) 160, 729
HindII GTYRAC 2 cut(s) 160, 729
HinfI GANTC 2 cut(s) 96, 638
HpaII CCGG 6 cut(s) 299, 321, 464, 482, 584, 670
HphI GGTGA 3 cut(s) 448, 502, 659
Hpy166II GTNNAC 3 cut(s) 160, 269, 729
Hpy188I TCNGA 3 cut(s) 290, 581, 591
Hpy188III TCNNGA 5 cut(s) 133, 505, 557, 635, 697
Hpy8I GTNNAC 3 cut(s) 160, 269, 729
HpyCH4III ACNGT 4 cut(s) 164, 208, 313, 726
HpyCH4V TGCA 2 cut(s) 172, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 3 cut(s) 28, 536, 654
Hsp92II CATG 4 cut(s) 136, 172, 443, 490
HspAI GCGC 1 cut(s) 279
Kzo9I GATC 2 cut(s) 285, 559
LmnI GCTCC 2 cut(s) 384, 685
LpnPI CCDG 8 cut(s) 158, 312, 334, 477, 495, 597, 683, 732
Lsp1109I GCAGC 1 cut(s) 231
LweI GCATC 1 cut(s) 254
MaeI CTAG 2 cut(s) 395, 530
MalI GATC 2 cut(s) 287, 561
MboI GATC 2 cut(s) 285, 559
MboII GAAGA 5 cut(s) 28, 77, 482, 505, 712
MhlI GDGCHC 1 cut(s) 544
MluCI AATT 3 cut(s) 188, 690, 742
MlyI GAGTC 1 cut(s) 632
MnlI CCTC 5 cut(s) 392, 503, 669, 673, 701
MroXI GAANNNNTTC 1 cut(s) 474
MseI TTAA 4 cut(s) 56, 203, 257, 600
MspI CCGG 6 cut(s) 299, 321, 464, 482, 584, 670
MspR9I CCNGG 2 cut(s) 465, 482
Mva1269I GAATGC 1 cut(s) 401
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 1 cut(s) 251
NciI CCSGG 2 cut(s) 465, 482
NdeI CATATG 1 cut(s) 386
NdeII GATC 2 cut(s) 285, 559
NlaIII CATG 4 cut(s) 136, 172, 443, 490
NlaIV GGNNCC 1 cut(s) 588
NspI RCATGY 1 cut(s) 443
PagI TCATGA 1 cut(s) 132
PctI GAATGC 1 cut(s) 401
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 1 cut(s) 96
PinAI ACCGGT 1 cut(s) 583
PkrI GCNGC 1 cut(s) 221
PleI GAGTC 1 cut(s) 632
PpsI GAGTC 1 cut(s) 632
PshBI ATTAAT 1 cut(s) 56
PspN4I GGNNCC 1 cut(s) 588
PspPI GGNCC 1 cut(s) 581
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
Rsr2I CGGWCCG 1 cut(s) 581
RsrII CGGWCCG 1 cut(s) 581
SalI GTCGAC 1 cut(s) 158
SaqAI TTAA 4 cut(s) 56, 203, 257, 600
SatI GCNGC 1 cut(s) 220
Sau3AI GATC 2 cut(s) 285, 559
Sau96I GGNCC 1 cut(s) 581
ScaI AGTACT 1 cut(s) 26
SchI GAGTC 1 cut(s) 632
ScrFI CCNGG 2 cut(s) 465, 482
SduI GDGCHC 1 cut(s) 544
SetI ASST 3 cut(s) 232, 296, 535
SfaNI GCATC 1 cut(s) 254
SfcI CTRYAG 1 cut(s) 91
SinI GGWCC 1 cut(s) 581
SmlI CTYRAG 1 cut(s) 505
SmoI CTYRAG 1 cut(s) 505
Sse9I AATT 3 cut(s) 188, 690, 742
SsiI CCGC 2 cut(s) 513, 683
SspMI CTAG 2 cut(s) 395, 530
StyD4I CCNGG 2 cut(s) 463, 480
TaaI ACNGT 4 cut(s) 164, 208, 313, 726
TaqI TCGA 4 cut(s) 141, 159, 215, 636
TasI AATT 3 cut(s) 188, 690, 742
TatI WGTACW 1 cut(s) 24
TfiI GAWTC 1 cut(s) 96
Tru1I TTAA 4 cut(s) 56, 203, 257, 600
Tru9I TTAA 4 cut(s) 56, 203, 257, 600
TseI GCWGC 1 cut(s) 219
TspDTI ATGAA 2 cut(s) 121, 149
VpaK11BI GGWCC 1 cut(s) 581
VspI ATTAAT 1 cut(s) 56
XceI RCATGY 1 cut(s) 443
XmiI GTMKAC 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 2 cut(s) 395, 530
ZrmI AGTACT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.