FvH4_3g08420

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004799
Physical Location & Seq
Forward (+)
4916792 .. 4918312
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g08420.t1

Sequence Viewer

Length: 756 bp
ATGGGTTTTGGGAAGGATGATCTTGATTTGGTGTTGGTCCCAAGTGGATTGCTGATCATGTTTGTCTACCACGTCAATCTTCTCTACAGATATCTTCATCTCCCTCACACCACAGTCATTGGTTTTGAAAACAATGACAAAAAAGCTTGGGTTGAAAGAGTTATGCAGGCTGAAAGAAGAGATATTGGAATAGTACTTAATGTGATTTCATCAAACACATCAGCAGCAACATTTTTGTGTTCCATTTCGCTGACTCTAAGTTCCCTTATTGGAGCTTGGCTTGGAAGCAACACAAATGAGGTCTTCCAGAGTGAATTAATCTACGGCAATACAAATCCATCAACGATGACCATCAAGTACATAAGCCTCTTAACTTGCTTCCTTCTCGCGTTCGCTTGCTTTGTTCAATCAGCAAGGCACTTTGTTCATGCCAACTACCTAATAAGTACACCAGACAGCAACATTCCAGCCTGGTATGTTGAGATGGCAGTTATAAGAGGAGGTGATTTTTGGTCACTTGGGCTAAGAGCTTTGTATTTTGCTCTCACTTTGCTTCTATGGTTTTTTGGTCCAATTCCAATGTTTGTGTCCTCCATTGTTCTGGTGATGGTCCTCCATTATCTTGACACAAACACCAGGCCATTGTTTCAGCATCAGTTTAAAGGAAAGGAAATAGTGAAGAGGGTGGAGGAGAGATTTTCAGAGGTTGTGACTGTAGAGCATCATCACAGAGAAAAGGCTGAAACTTTGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

252

Amino Acids

28.58

Weight (kDa)

6.54

Isoelectric Point (pI)

24.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 212 3.4e-62 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 494
AccI GTMKAC 1 cut(s) 66
AccII CGCG 1 cut(s) 389
AfaI GTAC 3 cut(s) 195, 359, 448
AgsI TTSAA 3 cut(s) 128, 155, 407
AjiI CACGTC 1 cut(s) 73
AjnI CCWGG 2 cut(s) 470, 635
AluBI AGCT 3 cut(s) 146, 275, 530
AluI AGCT 3 cut(s) 146, 275, 530
AoxI GGCC 1 cut(s) 638
ApeKI GCWGC 1 cut(s) 224
AseI ATTAAT 1 cut(s) 317
AspS9I GGNCC 3 cut(s) 37, 569, 610
AsuHPI GGTGA 2 cut(s) 515, 616
AvaII GGWCC 3 cut(s) 37, 569, 610
BbsI GAAGAC 1 cut(s) 295
BbvI GCAGC 1 cut(s) 236
BccI CCATC 4 cut(s) 346, 359, 478, 601
BceAI ACGGC 1 cut(s) 340
BciT130I CCWGG 2 cut(s) 472, 637
BclI TGATCA 1 cut(s) 54
BfmI CTRYAG 2 cut(s) 85, 714
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
BmcAI AGTACT 1 cut(s) 195
Bme1390I CCNGG 2 cut(s) 472, 637
Bme18I GGWCC 3 cut(s) 37, 569, 610
BmgBI CACGTC 1 cut(s) 73
BmgT120I GGNCC 3 cut(s) 37, 569, 610
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 2 cut(s) 472, 637
BmsI GCATC 2 cut(s) 661, 730
BpiI GAAGAC 1 cut(s) 295
BsaBI GATNNNNATC 1 cut(s) 350
Bse8I GATNNNNATC 1 cut(s) 350
BseBI CCWGG 2 cut(s) 472, 637
BseGI GGATG 1 cut(s) 22
BseJI GATNNNNATC 1 cut(s) 350
BseRI GAGGAG 2 cut(s) 513, 704
BseXI GCAGC 1 cut(s) 236
Bsh1236I CGCG 1 cut(s) 389
BshFI GGCC 1 cut(s) 640
BslFI GGGAC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 23
BsnI GGCC 1 cut(s) 640
Bsp143I GATC 2 cut(s) 19, 54
BspANI GGCC 1 cut(s) 640
BspFNI CGCG 1 cut(s) 389
BspLI GGNNCC 1 cut(s) 39
BssMI GATC 2 cut(s) 19, 54
Bst2UI CCWGG 2 cut(s) 472, 637
Bst4CI ACNGT 2 cut(s) 115, 715
Bst6I CTCTTC 2 cut(s) 172, 674
BstC8I GCNNGC 2 cut(s) 168, 397
BstDEI CTNAG 2 cut(s) 257, 524
BstF5I GGATG 1 cut(s) 22
BstFNI CGCG 1 cut(s) 389
BstKTI GATC 2 cut(s) 22, 57
BstMBI GATC 2 cut(s) 19, 54
BstNI CCWGG 2 cut(s) 472, 637
BstSCI CCNGG 2 cut(s) 470, 635
BstSFI CTRYAG 2 cut(s) 85, 714
BstUI CGCG 1 cut(s) 389
BstV1I GCAGC 1 cut(s) 236
BstV2I GAAGAC 1 cut(s) 295
BstXI CCANNNNNNTGG 1 cut(s) 601
BsuRI GGCC 1 cut(s) 640
BtrI CACGTC 1 cut(s) 73
BtsCI GGATG 1 cut(s) 22
Cac8I GCNNGC 2 cut(s) 168, 397
Cfr13I GGNCC 3 cut(s) 37, 569, 610
Csp6I GTAC 3 cut(s) 194, 358, 447
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 2 cut(s) 58, 428
CviQI GTAC 3 cut(s) 194, 358, 447
DdeI CTNAG 2 cut(s) 257, 524
DpnI GATC 2 cut(s) 21, 56
DpnII GATC 2 cut(s) 19, 54
DraI TTTAAA 1 cut(s) 661
Eam1104I CTCTTC 2 cut(s) 172, 674
EarI CTCTTC 2 cut(s) 172, 674
Eco32I GATATC 1 cut(s) 92
Eco47I GGWCC 3 cut(s) 37, 569, 610
EcoRII CCWGG 2 cut(s) 470, 635
EcoRV GATATC 1 cut(s) 92
FaeI CATG 2 cut(s) 61, 431
FaiI YATR 7 cut(s) 59, 164, 362, 429, 477, 494, 559
FaqI GGGAC 1 cut(s) 23
FatI CATG 2 cut(s) 57, 427
FbaI TGATCA 1 cut(s) 54
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 1 cut(s) 225
GluI GCNGC 1 cut(s) 225
HaeIII GGCC 1 cut(s) 640
Hin1II CATG 2 cut(s) 61, 431
HindIII AAGCTT 1 cut(s) 144
HinfI GANTC 1 cut(s) 253
HphI GGTGA 2 cut(s) 515, 616
Hpy166II GTNNAC 2 cut(s) 67, 449
Hpy188I TCNGA 1 cut(s) 703
Hpy188III TCNNGA 3 cut(s) 23, 307, 623
Hpy8I GTNNAC 2 cut(s) 67, 449
HpyAV CCTTC 2 cut(s) 7, 392
HpyCH4III ACNGT 2 cut(s) 115, 715
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 1 cut(s) 166
HpyF3I CTNAG 2 cut(s) 257, 524
HpySE526I ACGT 1 cut(s) 72
Hsp92II CATG 2 cut(s) 61, 431
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 2 cut(s) 19, 54
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 9 cut(s) 152, 320, 457, 465, 480, 484, 587, 622, 649
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 2 cut(s) 661, 730
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 2 cut(s) 513, 709
MalI GATC 2 cut(s) 21, 56
MboI GATC 2 cut(s) 19, 54
MboII GAAGA 5 cut(s) 71, 86, 189, 295, 691
MluCI AATT 2 cut(s) 314, 573
MlyI GAGTC 1 cut(s) 247
MseI TTAA 4 cut(s) 198, 317, 371, 660
MslI CAYNNNNRTG 1 cut(s) 235
MspR9I CCNGG 2 cut(s) 472, 637
MvaI CCWGG 2 cut(s) 472, 637
MvnI CGCG 1 cut(s) 389
NdeII GATC 2 cut(s) 19, 54
NlaIII CATG 2 cut(s) 61, 431
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 513, 709
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PshBI ATTAAT 1 cut(s) 317
PsiI TTATAA 1 cut(s) 494
Psp6I CCWGG 2 cut(s) 470, 635
PspGI CCWGG 2 cut(s) 470, 635
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 3 cut(s) 37, 569, 610
RsaI GTAC 3 cut(s) 195, 359, 448
RsaNI GTAC 3 cut(s) 194, 358, 447
RseI CAYNNNNRTG 1 cut(s) 235
SaqAI TTAA 4 cut(s) 198, 317, 371, 660
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 2 cut(s) 19, 54
Sau96I GGNCC 3 cut(s) 37, 569, 610
ScaI AGTACT 1 cut(s) 195
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 2 cut(s) 472, 637
SetI ASST 8 cut(s) 75, 148, 277, 303, 441, 505, 532, 708
SfaNI GCATC 2 cut(s) 661, 730
SfcI CTRYAG 2 cut(s) 85, 714
SinI GGWCC 3 cut(s) 37, 569, 610
SmiMI CAYNNNNRTG 1 cut(s) 235
Sse9I AATT 2 cut(s) 314, 573
StyD4I CCNGG 2 cut(s) 470, 635
TaaI ACNGT 2 cut(s) 115, 715
TaiI ACGT 1 cut(s) 75
TasI AATT 2 cut(s) 314, 573
TatI WGTACW 3 cut(s) 193, 357, 446
Tru1I TTAA 4 cut(s) 198, 317, 371, 660
Tru9I TTAA 4 cut(s) 198, 317, 371, 660
TseFI GTSAC 2 cut(s) 513, 709
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 2 cut(s) 513, 709
TspDTI ATGAA 3 cut(s) 86, 198, 416
VpaK11BI GGWCC 3 cut(s) 37, 569, 610
VspI ATTAAT 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 66
ZrmI AGTACT 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.