MD05G1308900.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004799
Physical Location & Seq
Forward (+)
43985292 .. 43986283
992 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1308900.v1.1.491

Sequence Viewer

Length: 771 bp
ATGAATTTTGGAAAGGAGGACCTTGATCTGGTTTTGGTCCCAAGTGGACTGTTCATCATGCTTGTCTATCACATCATCCTCCTCTACAGATATCTCCATCAACCTCACACCACAGTCCTTGGCTTTGAAAACAATGACAAAAGAGCTTGGGCTGAAAGAATTATGCAGGTTGACAAGAGAGATGTTGGCACAGCCTTAAATGTGATCTCATCCAACACATCAGCAGCAACTTTTCTGTGTTCCATCTCCTTGACTCTCAGCTCCCTCATTGGAGCTTGGCTCGGAAGTAATTCAACTAAGACAGTCTTCCAGAGTGAATTGATCTATGGCAACACCAACCCATCAATCCTCACAATAAAATACCTAAGCCTCTTGACTTGTTTTCTTCTAGCCTTTGCATGCTTTGTTCAATCAGCAAGGCACTTTGTCCATGCAAACTACCTGATCAGCATGCCGGATAGTAACATTCCGGCATGGTACGTGCAGATGGCGGTGATAAGGGGAAGTGATTTCTGGTCACTTGGGCTTAGAGCACTCTATTTTGCTCTTACTCTTTTGCTGTGGTTTTTTGGTCCGATTCCGATGTTTGTTTCCTCCATGGTTTTGGTCATAGTTCTACATAACCTTGACACAAACACCAGACCATTGCATCAGCATCAGCTTCCGGGAAAGGAGCTCGTTAAACACACCGGCGAGAGAATCTCAGAGGTGGCTGTGACCATTCAGCATCATACAGAAACGGTTAAAACTACGACTAGTAGTACTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

257

Amino Acids

28.73

Weight (kDa)

7.82

Isoelectric Point (pI)

27.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 213 9.8e-63 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 157
AciI CCGC 1 cut(s) 491
AcsI RAATTY 1 cut(s) 4
AfaI GTAC 2 cut(s) 479, 763
AfiI CCNNNNNNNGG 1 cut(s) 28
AgsI TTSAA 3 cut(s) 128, 294, 410
AhlI ACTAGT 1 cut(s) 755
AluBI AGCT 5 cut(s) 146, 261, 275, 661, 676
AluI AGCT 5 cut(s) 146, 261, 275, 661, 676
Alw21I GWGCWC 2 cut(s) 535, 678
ApeKI GCWGC 1 cut(s) 224
ApoI RAATTY 1 cut(s) 4
Asp700I GAANNNNTTC 1 cut(s) 289
AspS9I GGNCC 3 cut(s) 19, 37, 572
AsuC2I CCSGG 1 cut(s) 666
AsuHPI GGTGA 1 cut(s) 505
AvaII GGWCC 3 cut(s) 19, 37, 572
BanII GRGCYC 1 cut(s) 678
BbsI GAAGAC 1 cut(s) 298
Bbv12I GWGCWC 2 cut(s) 535, 678
BbvI GCAGC 1 cut(s) 236
BccI CCATC 4 cut(s) 105, 251, 349, 481
BclI TGATCA 1 cut(s) 444
BcnI CCSGG 1 cut(s) 666
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 2 cut(s) 389, 756
BfmI CTRYAG 1 cut(s) 85
BfuAI ACCTGC 1 cut(s) 157
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
BmcAI AGTACT 1 cut(s) 763
Bme1390I CCNGG 1 cut(s) 666
Bme18I GGWCC 3 cut(s) 19, 37, 572
BmgT120I GGNCC 3 cut(s) 19, 37, 572
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 666
BmsI GCATC 3 cut(s) 658, 664, 736
BpiI GAAGAC 1 cut(s) 298
BplI GAGNNNNNCTC 4 cut(s) 264, 296, 686, 718
Bpu10I CCTNAGC 1 cut(s) 365
BpuMI CCSGG 1 cut(s) 666
BsaAI YACGTR 1 cut(s) 481
BsaJI CCNNGG 2 cut(s) 118, 597
Bsc4I CCNNNNNNNGG 1 cut(s) 28
Bse118I RCCGGY 1 cut(s) 689
Bse3DI GCAATG 1 cut(s) 644
BseDI CCNNGG 2 cut(s) 118, 597
BseGI GGATG 2 cut(s) 75, 209
BseLI CCNNNNNNNGG 1 cut(s) 28
BseMI GCAATG 1 cut(s) 644
BseMII CTCAG 2 cut(s) 271, 717
BseRI GAGGAG 1 cut(s) 71
BseXI GCAGC 1 cut(s) 236
BsgI GTGCAG 1 cut(s) 503
BsiHKAI GWGCWC 2 cut(s) 535, 678
BsiSI CCGG 4 cut(s) 455, 470, 665, 690
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 23
Bsp1286I GDGCHC 2 cut(s) 535, 678
Bsp143I GATC 4 cut(s) 25, 204, 321, 444
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 1 cut(s) 491
BspCNI CTCAG 2 cut(s) 270, 716
BspLI GGNNCC 1 cut(s) 39
BspMI ACCTGC 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 644
BsrFI RCCGGY 1 cut(s) 689
BssAI RCCGGY 1 cut(s) 689
BssECI CCNNGG 2 cut(s) 118, 597
BssMI GATC 4 cut(s) 25, 204, 321, 444
BssT1I CCWWGG 2 cut(s) 118, 597
Bst4CI ACNGT 4 cut(s) 51, 115, 304, 742
BstBAI YACGTR 1 cut(s) 481
BstC8I GCNNGC 2 cut(s) 400, 452
BstDEI CTNAG 5 cut(s) 257, 297, 365, 527, 703
BstDSI CCRYGG 1 cut(s) 597
BstF5I GGATG 2 cut(s) 75, 209
BstKTI GATC 4 cut(s) 28, 207, 324, 447
BstMBI GATC 4 cut(s) 25, 204, 321, 444
BstNSI RCATGY 2 cut(s) 402, 454
BstSCI CCNGG 1 cut(s) 664
BstSFI CTRYAG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 236
BstV2I GAAGAC 1 cut(s) 298
BstXI CCANNNNNNTGG 1 cut(s) 604
BtgI CCRYGG 1 cut(s) 597
BtsCI GGATG 2 cut(s) 75, 209
BveI ACCTGC 1 cut(s) 157
Cac8I GCNNGC 2 cut(s) 400, 452
Cfr10I RCCGGY 1 cut(s) 689
Cfr13I GGNCC 3 cut(s) 19, 37, 572
Csp6I GTAC 2 cut(s) 478, 762
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 6 cut(s) 58, 399, 431, 451, 474, 598
CviQI GTAC 2 cut(s) 478, 762
DdeI CTNAG 5 cut(s) 257, 297, 365, 527, 703
DpnI GATC 4 cut(s) 27, 206, 323, 446
DpnII GATC 4 cut(s) 25, 204, 321, 444
Ecl136II GAGCTC 1 cut(s) 676
Eco130I CCWWGG 2 cut(s) 118, 597
Eco24I GRGCYC 1 cut(s) 678
Eco32I GATATC 1 cut(s) 92
Eco47I GGWCC 3 cut(s) 19, 37, 572
Eco53kI GAGCTC 1 cut(s) 676
EcoICRI GAGCTC 1 cut(s) 676
EcoO109I RGGNCCY 1 cut(s) 19
EcoRV GATATC 1 cut(s) 92
EcoT14I CCWWGG 2 cut(s) 118, 597
EcoT38I GRGCYC 1 cut(s) 678
ErhI CCWWGG 2 cut(s) 118, 597
FaeI CATG 6 cut(s) 61, 402, 434, 454, 477, 601
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FaqI GGGAC 1 cut(s) 23
FatI CATG 6 cut(s) 57, 398, 430, 450, 473, 597
FbaI TGATCA 1 cut(s) 444
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 2 cut(s) 62, 196
FriOI GRGCYC 1 cut(s) 678
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 2 cut(s) 389, 756
GluI GCNGC 1 cut(s) 225
HapII CCGG 4 cut(s) 455, 470, 665, 690
Hin1II CATG 6 cut(s) 61, 402, 434, 454, 477, 601
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 3 cut(s) 253, 577, 699
HpaII CCGG 4 cut(s) 455, 470, 665, 690
HphI GGTGA 1 cut(s) 505
Hpy166II GTNNAC 2 cut(s) 47, 172
Hpy188I TCNGA 4 cut(s) 284, 576, 582, 706
Hpy188III TCNNGA 2 cut(s) 310, 373
Hpy8I GTNNAC 2 cut(s) 47, 172
HpyCH4III ACNGT 4 cut(s) 51, 115, 304, 742
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 5 cut(s) 166, 398, 434, 484, 649
HpyF3I CTNAG 5 cut(s) 257, 297, 365, 527, 703
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 6 cut(s) 61, 402, 434, 454, 477, 601
Ksp22I TGATCA 1 cut(s) 444
Kzo9I GATC 4 cut(s) 25, 204, 321, 444
LmnI GCTCC 3 cut(s) 266, 272, 673
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 3 cut(s) 658, 664, 736
MaeI CTAG 2 cut(s) 389, 756
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 3 cut(s) 461, 516, 715
MalI GATC 4 cut(s) 27, 206, 323, 446
MboI GATC 4 cut(s) 25, 204, 321, 444
MboII GAAGA 2 cut(s) 298, 377
MhlI GDGCHC 2 cut(s) 535, 678
MluCI AATT 4 cut(s) 4, 159, 289, 317
MlyI GAGTC 1 cut(s) 247
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 9 cut(s) 10, 89, 92, 114, 275, 359, 380, 604, 700
MroXI GAANNNNTTC 1 cut(s) 289
MseI TTAA 3 cut(s) 197, 681, 744
MspI CCGG 4 cut(s) 455, 470, 665, 690
MspR9I CCNGG 1 cut(s) 666
NciI CCSGG 1 cut(s) 666
NcoI CCATGG 1 cut(s) 597
NdeII GATC 4 cut(s) 25, 204, 321, 444
NlaIII CATG 6 cut(s) 61, 402, 434, 454, 477, 601
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 516, 715
NspI RCATGY 2 cut(s) 402, 454
PaeI GCATGC 2 cut(s) 402, 454
PdmI GAANNNNTTC 1 cut(s) 289
PfeI GAWTC 2 cut(s) 577, 699
PfoI TCCNGGA 1 cut(s) 664
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
Ppu21I YACGTR 1 cut(s) 481
PpuMI RGGWCCY 1 cut(s) 19
Psp124BI GAGCTC 1 cut(s) 678
Psp5II RGGWCCY 1 cut(s) 19
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 3 cut(s) 19, 37, 572
PspPPI RGGWCCY 1 cut(s) 19
RsaI GTAC 2 cut(s) 479, 763
RsaNI GTAC 2 cut(s) 478, 762
SacI GAGCTC 1 cut(s) 678
SaqAI TTAA 3 cut(s) 197, 681, 744
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 4 cut(s) 25, 204, 321, 444
Sau96I GGNCC 3 cut(s) 19, 37, 572
ScaI AGTACT 1 cut(s) 763
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 1 cut(s) 666
SduI GDGCHC 2 cut(s) 535, 678
SfaNI GCATC 3 cut(s) 658, 664, 736
SfcI CTRYAG 1 cut(s) 85
SgrAI CRCCGGYG 1 cut(s) 689
SinI GGWCC 3 cut(s) 19, 37, 572
SpeI ACTAGT 1 cut(s) 755
SphI GCATGC 2 cut(s) 402, 454
Sse9I AATT 4 cut(s) 4, 159, 289, 317
SsiI CCGC 1 cut(s) 491
SspMI CTAG 2 cut(s) 389, 756
SstI GAGCTC 1 cut(s) 678
StyD4I CCNGG 1 cut(s) 664
StyI CCWWGG 2 cut(s) 118, 597
TaaI ACNGT 4 cut(s) 51, 115, 304, 742
TaiI ACGT 1 cut(s) 483
TasI AATT 4 cut(s) 4, 159, 289, 317
TatI WGTACW 1 cut(s) 761
TfiI GAWTC 2 cut(s) 577, 699
Tru1I TTAA 3 cut(s) 197, 681, 744
Tru9I TTAA 3 cut(s) 197, 681, 744
TseFI GTSAC 2 cut(s) 516, 715
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 2 cut(s) 516, 715
TspDTI ATGAA 2 cut(s) 17, 43
VpaK11BI GGWCC 3 cut(s) 19, 37, 572
XapI RAATTY 1 cut(s) 4
XceI RCATGY 2 cut(s) 402, 454
XmnI GAANNNNTTC 1 cut(s) 289
XspI CTAG 2 cut(s) 389, 756
ZrmI AGTACT 1 cut(s) 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.