Rh5AG224000

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004799
Physical Location & Seq
Forward (+)
26651991 .. 26653307
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG224000.1

Sequence Viewer

Length: 864 bp
ATGGTTTGGAAGAAGGAATACTTGGATTTAATCTTGGTCCCAACTGGGTTGCTAATCATGTTATGCTACCATCTCTTTCTCCTCTACAGATGCCTAAGGCATCCTGAAACCACAGTCATTGGCAATGAAAACCACTGTAGAAAAGCTTGGGTTGAGAGAATGTTGCAGATTGAAGCAAAGGATCGAGGTCAGTCCCTAGCAGTAATCTCCAGCACTATAACAGCAGCAAATTTCTTGGCTTCAACCTCTTTAGCCTTGAGTTCTTTGATTGGAACATGGGTTCTAAACTCTTCAAACAGCATCCTCCTCACTAGTTTTACGTATGGTGACACAAGTCCTGCAATGAATTCTATTAAATACGTTAGCATTCTGGCCTGCTTCCTATTAGCTCTAGCTTCTTTCTTACACTGTATAAGAAGCTTTATCCATGCCACTTTCCTCATGAGCATGCCAAACAGTGATACTCCTCTGAGTGATGTTCAGAAGGCTGTGATACATGGCGGCCTTTTTTGGACATTGGGGTTGAGAGCAATCTATTTTGCGACTAATTTACTCCTCTGGGTTTTTGGTCCAATTCCGATGTTTGTGTGTTCAGTGACTTTGGTGTTGATTCTGAACAAGCTGGATTCGAATTCCACACCATTGCATCAGTTCCAACCAGCCAGGAGTCAGGACAATAGGTTGATGGGGAAACTTGCTGAAGAAAAAACCCAAATAGATAGGGTTATCGAGCAACACGAAAGATCACACATAGATGATAACAGATCTCAAGGTTCTAACTGTCAACAATCACCTGCCTCTGGTTTGCAGAAAGCACAAAATTATGGGCAGCTTCTTCAAAGGCAGAAGCCCTTTTCTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

287

Amino Acids

32.19

Weight (kDa)

8.84

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 213 1.2e-57 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 802
Acc36I ACCTGC 1 cut(s) 802
AciI CCGC 1 cut(s) 501
AclWI GGATC 1 cut(s) 189
AcsI RAATTY 3 cut(s) 229, 346, 631
AcuI CTGAAG 1 cut(s) 720
AfiI CCNNNNNNNGG 1 cut(s) 800
AgsI TTSAA 4 cut(s) 173, 243, 294, 839
AhlI ACTAGT 1 cut(s) 311
AjnI CCWGG 1 cut(s) 662
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AloI GAACNNNNNNTCC 2 cut(s) 264, 296
AluBI AGCT 6 cut(s) 146, 389, 395, 420, 622, 832
AluI AGCT 6 cut(s) 146, 389, 395, 420, 622, 832
AlwI GGATC 1 cut(s) 189
AoxI GGCC 2 cut(s) 372, 502
ApeKI GCWGC 2 cut(s) 224, 829
ApoI RAATTY 3 cut(s) 229, 346, 631
AspS9I GGNCC 2 cut(s) 37, 569
AsuHPI GGTGA 2 cut(s) 338, 783
AsuII TTCGAA 1 cut(s) 629
AvaII GGWCC 2 cut(s) 37, 569
AxyI CCTNAGG 1 cut(s) 95
BarI GAAGNNNNNNTAC 1 cut(s) 34
BbvI GCAGC 2 cut(s) 236, 841
BccI CCATC 2 cut(s) 78, 679
BciT130I CCWGG 1 cut(s) 664
BcuI ACTAGT 1 cut(s) 311
BfaI CTAG 3 cut(s) 197, 312, 392
BfmI CTRYAG 2 cut(s) 85, 136
BfuAI ACCTGC 1 cut(s) 802
BglII AGATCT 1 cut(s) 764
BisI GCNGC 3 cut(s) 225, 502, 830
BlsI GCNGC 3 cut(s) 226, 503, 831
Bme1390I CCNGG 1 cut(s) 664
Bme18I GGWCC 2 cut(s) 37, 569
BmgT120I GGNCC 2 cut(s) 37, 569
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 664
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 4 cut(s) 80, 109, 309, 655
BmuI ACTGGG 1 cut(s) 54
BoxI GACNNNNGTC 1 cut(s) 333
BpmI CTGGAG 1 cut(s) 193
Bpu14I TTCGAA 1 cut(s) 629
BpuEI CTTGAG 2 cut(s) 277, 753
BsaAI YACGTR 1 cut(s) 321
Bsc4I CCNNNNNNNGG 1 cut(s) 800
Bse1I ACTGG 1 cut(s) 49
Bse21I CCTNAGG 1 cut(s) 95
Bse3DI GCAATG 3 cut(s) 130, 348, 641
BseBI CCWGG 1 cut(s) 664
BseGI GGATG 2 cut(s) 100, 300
BseLI CCNNNNNNNGG 1 cut(s) 800
BseMI GCAATG 3 cut(s) 130, 348, 641
BseMII CTCAG 1 cut(s) 461
BseNI ACTGG 1 cut(s) 49
BseRI GAGGAG 4 cut(s) 71, 296, 456, 545
BseXI GCAGC 2 cut(s) 236, 841
BshFI GGCC 2 cut(s) 374, 504
BslFI GGGAC 2 cut(s) 23, 178
BslI CCNNNNNNNGG 1 cut(s) 800
BsmFI GGGAC 2 cut(s) 23, 178
BsmI GAATGC 1 cut(s) 366
BsnI GGCC 2 cut(s) 374, 504
Bsp119I TTCGAA 1 cut(s) 629
Bsp143I GATC 3 cut(s) 181, 743, 764
BspACI CCGC 1 cut(s) 501
BspANI GGCC 2 cut(s) 374, 504
BspCNI CTCAG 1 cut(s) 462
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 39
BspMI ACCTGC 1 cut(s) 802
BspPI GGATC 1 cut(s) 189
BspT104I TTCGAA 1 cut(s) 629
BsrDI GCAATG 3 cut(s) 130, 348, 641
BsrI ACTGG 1 cut(s) 49
BssMI GATC 3 cut(s) 181, 743, 764
Bst2UI CCWGG 1 cut(s) 664
Bst4CI ACNGT 5 cut(s) 115, 137, 410, 458, 782
Bst6I CTCTTC 1 cut(s) 295
BstBAI YACGTR 1 cut(s) 321
BstBI TTCGAA 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 376, 449
BstDEI CTNAG 2 cut(s) 95, 470
BstF5I GGATG 2 cut(s) 100, 300
BstKTI GATC 3 cut(s) 184, 746, 767
BstMBI GATC 3 cut(s) 181, 743, 764
BstNI CCWGG 1 cut(s) 664
BstNSI RCATGY 1 cut(s) 451
BstPAI GACNNNNGTC 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 662
BstSFI CTRYAG 2 cut(s) 85, 136
BstSNI TACGTA 1 cut(s) 321
BstV1I GCAGC 2 cut(s) 236, 841
BstX2I RGATCY 1 cut(s) 764
BstYI RGATCY 1 cut(s) 764
Bsu36I CCTNAGG 1 cut(s) 95
BsuRI GGCC 2 cut(s) 374, 504
BtsCI GGATG 2 cut(s) 100, 300
BtsIMutI CAGTG 4 cut(s) 133, 406, 463, 600
BveI ACCTGC 1 cut(s) 802
Cac8I GCNNGC 2 cut(s) 376, 449
CciI TCATGA 1 cut(s) 441
Cfr13I GGNCC 2 cut(s) 37, 569
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 6 cut(s) 58, 276, 428, 442, 448, 497
DdeI CTNAG 2 cut(s) 95, 470
DpnI GATC 3 cut(s) 183, 745, 766
DpnII GATC 3 cut(s) 181, 743, 764
Eam1104I CTCTTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 295
Eco105I TACGTA 1 cut(s) 321
Eco47I GGWCC 2 cut(s) 37, 569
Eco57I CTGAAG 1 cut(s) 720
Eco81I CCTNAGG 1 cut(s) 95
EcoRI GAATTC 2 cut(s) 346, 631
EcoRII CCWGG 1 cut(s) 662
FaeI CATG 6 cut(s) 61, 279, 431, 445, 451, 500
FalI AAGNNNNNCTT 1 cut(s) 37
FaqI GGGAC 2 cut(s) 23, 178
FatI CATG 6 cut(s) 57, 275, 427, 441, 447, 496
Fnu4HI GCNGC 3 cut(s) 225, 502, 830
FokI GGATG 2 cut(s) 87, 287
Fsp4HI GCNGC 3 cut(s) 225, 502, 830
FspBI CTAG 3 cut(s) 197, 312, 392
GluI GCNGC 3 cut(s) 225, 502, 830
GsuI CTGGAG 1 cut(s) 193
HaeIII GGCC 2 cut(s) 374, 504
Hin1II CATG 6 cut(s) 61, 279, 431, 445, 451, 500
HincII GTYRAC 1 cut(s) 785
HindII GTYRAC 1 cut(s) 785
HindIII AAGCTT 2 cut(s) 144, 418
HinfI GANTC 3 cut(s) 610, 626, 667
HphI GGTGA 2 cut(s) 338, 783
Hpy166II GTNNAC 1 cut(s) 785
Hpy188I TCNGA 4 cut(s) 471, 483, 579, 615
Hpy188III TCNNGA 3 cut(s) 104, 442, 671
Hpy8I GTNNAC 1 cut(s) 785
HpyAV CCTTC 2 cut(s) 7, 478
HpyCH4III ACNGT 5 cut(s) 115, 137, 410, 458, 782
HpyCH4IV ACGT 2 cut(s) 320, 360
HpyCH4V TGCA 4 cut(s) 166, 341, 646, 808
HpyF3I CTNAG 2 cut(s) 95, 470
HpySE526I ACGT 2 cut(s) 320, 360
Hsp92II CATG 6 cut(s) 61, 279, 431, 445, 451, 500
Kzo9I GATC 3 cut(s) 181, 743, 764
Lsp1109I GCAGC 2 cut(s) 236, 841
LweI GCATC 4 cut(s) 80, 109, 309, 655
MaeI CTAG 3 cut(s) 197, 312, 392
MaeII ACGT 2 cut(s) 320, 360
MaeIII GTNAC 2 cut(s) 326, 595
MalI GATC 3 cut(s) 183, 745, 766
MboI GATC 3 cut(s) 181, 743, 764
MboII GAAGA 5 cut(s) 22, 282, 713, 827, 849
MflI RGATCY 1 cut(s) 764
MluCI AATT 6 cut(s) 229, 346, 547, 573, 631, 820
MlyI GAGTC 1 cut(s) 676
MmeI TCCRAC 1 cut(s) 679
MnlI CCTC 9 cut(s) 92, 179, 256, 314, 317, 449, 477, 566, 808
MseI TTAA 2 cut(s) 29, 354
MslI CAYNNNNRTG 2 cut(s) 446, 753
MspR9I CCNGG 1 cut(s) 664
Mva1269I GAATGC 1 cut(s) 366
MvaI CCWGG 1 cut(s) 664
NdeII GATC 3 cut(s) 181, 743, 764
NlaIII CATG 6 cut(s) 61, 279, 431, 445, 451, 500
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 326, 595
NspI RCATGY 1 cut(s) 451
NspV TTCGAA 1 cut(s) 629
PaeI GCATGC 1 cut(s) 451
PagI TCATGA 1 cut(s) 441
PaqCI CACCTGC 1 cut(s) 802
PcsI WCGNNNNNNNCGW 1 cut(s) 735
PctI GAATGC 1 cut(s) 366
PfeI GAWTC 2 cut(s) 610, 626
PkrI GCNGC 3 cut(s) 226, 503, 831
PleI GAGTC 1 cut(s) 675
PpsI GAGTC 1 cut(s) 675
Ppu21I YACGTR 1 cut(s) 321
PshAI GACNNNNGTC 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 662
PspGI CCWGG 1 cut(s) 662
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 2 cut(s) 37, 569
PsuI RGATCY 1 cut(s) 764
RseI CAYNNNNRTG 2 cut(s) 446, 753
SaqAI TTAA 2 cut(s) 29, 354
SatI GCNGC 3 cut(s) 225, 502, 830
Sau3AI GATC 3 cut(s) 181, 743, 764
Sau96I GGNCC 2 cut(s) 37, 569
SchI GAGTC 1 cut(s) 676
ScrFI CCNGG 1 cut(s) 664
SfaNI GCATC 4 cut(s) 80, 109, 309, 655
SfcI CTRYAG 2 cut(s) 85, 136
SfuI TTCGAA 1 cut(s) 629
SinI GGWCC 2 cut(s) 37, 569
SmiMI CAYNNNNRTG 2 cut(s) 446, 753
SmlI CTYRAG 2 cut(s) 256, 768
SmoI CTYRAG 2 cut(s) 256, 768
SnaBI TACGTA 1 cut(s) 321
SpeI ACTAGT 1 cut(s) 311
SphI GCATGC 1 cut(s) 451
Sse9I AATT 6 cut(s) 229, 346, 547, 573, 631, 820
SsiI CCGC 1 cut(s) 501
SspMI CTAG 3 cut(s) 197, 312, 392
StyD4I CCNGG 1 cut(s) 662
TaaI ACNGT 5 cut(s) 115, 137, 410, 458, 782
TaiI ACGT 2 cut(s) 323, 363
TaqI TCGA 3 cut(s) 184, 629, 729
TasI AATT 6 cut(s) 229, 346, 547, 573, 631, 820
TauI GCSGC 1 cut(s) 504
TfiI GAWTC 2 cut(s) 610, 626
Tru1I TTAA 2 cut(s) 29, 354
Tru9I TTAA 2 cut(s) 29, 354
TscAI CASTG 4 cut(s) 140, 413, 463, 600
TseFI GTSAC 2 cut(s) 326, 595
TseI GCWGC 2 cut(s) 224, 829
Tsp45I GTSAC 2 cut(s) 326, 595
TspDTI ATGAA 3 cut(s) 141, 359, 849
TspRI CASTG 4 cut(s) 140, 413, 463, 600
VpaK11BI GGWCC 2 cut(s) 37, 569
XapI RAATTY 3 cut(s) 229, 346, 631
XceI RCATGY 1 cut(s) 451
XspI CTAG 3 cut(s) 197, 312, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.