MD10G1287600.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004799
Physical Location & Seq
Forward (+)
37682845 .. 37683850
1006 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1287600.v1.1.491

Sequence Viewer

Length: 768 bp
ATGAGTTTTGAAAAGGAGGACCTTGATCTGGTTTTGGTCCCAAGTGGATTGCTAATCATGTTTGTCTATCACATCATCCTTCTCTACAGATATATCCATCTCCCTCATACCACAGTTCTTGGCTTTGAAAACCATGACAAAAGAGCTTGGGTTGAAAGAATCATGCAGGTTGATAAAAGAGATGTAGGCACAGCCCTAAGTGTGATTTCATCCAACACATCAGCAGCAACTTTTCTGTGTTCCATCTCCTTAACTCTCAGCTCCCTCATCGGAGCTTGGATCGGAAGTAATTCGAGTCAGACAGTCTTCCAGAGTGAATTAATTTATGGAAGCACCGACCCATCAATCCTCACAATGAAATACATAAGCCTCTTAACTTGTTTCCTTCTAGCCTTCGCATGCTTTGTTCAATCAGCAAGGCACTTTGTCCATGCAAACTATCTGATCAGCATGCCGGACAGTAACATTCCGGCGTGGTACGTGCAGATGGCGGTCATAAGGGGAGGTGATTTCTGGTCACTTGGGCTTAGAGCACTCTATTTTTCTTTCACTCTTTTGCTATGGTTTTTCGGTCCGATTCCAATGTTTATTTCCTCCATGGTTTTGGTCATGGTCCTCCATTACCTTGACACAAACACCAGGCCACTGCACCAGCACCAGCTTCCCGGAAAGGAGATCATCAAACGCGTCGGAGAAAGGATCTCAGAGGTGGCTGTGACCATTCAGCGTCATACAGAAAGGGTTGAAACTACGAGAACTACTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

256

Amino Acids

28.95

Weight (kDa)

7.16

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 213 3.2e-62 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 157
AccII CGCG 1 cut(s) 687
AciI CCGC 1 cut(s) 491
AclWI GGATC 2 cut(s) 287, 707
AfaI GTAC 1 cut(s) 479
AfiI CCNNNNNNNGG 1 cut(s) 28
AflIII ACRYGT 1 cut(s) 685
AgsI TTSAA 5 cut(s) 11, 128, 155, 410, 746
AjnI CCWGG 1 cut(s) 638
AjuI GAANNNNNNNTTGG 2 cut(s) 574, 606
AluBI AGCT 4 cut(s) 146, 261, 275, 661
AluI AGCT 4 cut(s) 146, 261, 275, 661
Alw21I GWGCWC 1 cut(s) 535
AlwI GGATC 2 cut(s) 287, 707
AoxI GGCC 1 cut(s) 641
ApeKI GCWGC 1 cut(s) 224
AseI ATTAAT 1 cut(s) 320
Asp700I GAANNNNTTC 1 cut(s) 289
AspS9I GGNCC 4 cut(s) 19, 37, 572, 613
AsuC2I CCSGG 1 cut(s) 666
AsuHPI GGTGA 1 cut(s) 518
AvaII GGWCC 4 cut(s) 19, 37, 572, 613
BbsI GAAGAC 1 cut(s) 298
Bbv12I GWGCWC 1 cut(s) 535
BbvI GCAGC 1 cut(s) 236
BccI CCATC 4 cut(s) 105, 251, 349, 481
BciT130I CCWGG 1 cut(s) 640
BclI TGATCA 1 cut(s) 444
BcnI CCSGG 1 cut(s) 666
BfaI CTAG 1 cut(s) 389
BfmI CTRYAG 1 cut(s) 85
BfuAI ACCTGC 1 cut(s) 157
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
Bme1390I CCNGG 2 cut(s) 640, 666
Bme18I GGWCC 4 cut(s) 19, 37, 572, 613
BmgT120I GGNCC 4 cut(s) 19, 37, 572, 613
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 2 cut(s) 640, 666
BpiI GAAGAC 1 cut(s) 298
BpuMI CCSGG 1 cut(s) 666
BsaAI YACGTR 1 cut(s) 481
BsaJI CCNNGG 1 cut(s) 597
Bsc4I CCNNNNNNNGG 1 cut(s) 28
BseBI CCWGG 1 cut(s) 640
BseDI CCNNGG 1 cut(s) 597
BseGI GGATG 2 cut(s) 75, 209
BseLI CCNNNNNNNGG 1 cut(s) 28
BseMII CTCAG 2 cut(s) 271, 717
BseXI GCAGC 1 cut(s) 236
BsgI GTGCAG 2 cut(s) 503, 632
Bsh1236I CGCG 1 cut(s) 687
BshFI GGCC 1 cut(s) 643
BsiHKAI GWGCWC 1 cut(s) 535
BsiSI CCGG 3 cut(s) 455, 470, 666
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 23
BsnI GGCC 1 cut(s) 643
Bsp1286I GDGCHC 1 cut(s) 535
Bsp143I GATC 5 cut(s) 25, 279, 444, 675, 699
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 1 cut(s) 491
BspANI GGCC 1 cut(s) 643
BspCNI CTCAG 2 cut(s) 270, 716
BspFNI CGCG 1 cut(s) 687
BspLI GGNNCC 1 cut(s) 39
BspMI ACCTGC 1 cut(s) 157
BspPI GGATC 2 cut(s) 287, 707
BssECI CCNNGG 1 cut(s) 597
BssMI GATC 5 cut(s) 25, 279, 444, 675, 699
BssT1I CCWWGG 1 cut(s) 597
Bst2UI CCWGG 1 cut(s) 640
Bst4CI ACNGT 3 cut(s) 115, 304, 461
BstBAI YACGTR 1 cut(s) 481
BstC8I GCNNGC 2 cut(s) 400, 452
BstDEI CTNAG 4 cut(s) 197, 257, 527, 703
BstDSI CCRYGG 1 cut(s) 597
BstF5I GGATG 2 cut(s) 75, 209
BstFNI CGCG 1 cut(s) 687
BstKTI GATC 5 cut(s) 28, 282, 447, 678, 702
BstMBI GATC 5 cut(s) 25, 279, 444, 675, 699
BstNI CCWGG 1 cut(s) 640
BstNSI RCATGY 2 cut(s) 402, 454
BstSCI CCNGG 2 cut(s) 638, 664
BstSFI CTRYAG 1 cut(s) 85
BstUI CGCG 1 cut(s) 687
BstV1I GCAGC 1 cut(s) 236
BstV2I GAAGAC 1 cut(s) 298
BstX2I RGATCY 1 cut(s) 699
BstXI CCANNNNNNTGG 1 cut(s) 604
BstYI RGATCY 1 cut(s) 699
BsuRI GGCC 1 cut(s) 643
BtgI CCRYGG 1 cut(s) 597
BtsCI GGATG 2 cut(s) 75, 209
BtsI GCAGTG 1 cut(s) 644
BtsIMutI CAGTG 1 cut(s) 644
BveI ACCTGC 1 cut(s) 157
Cac8I GCNNGC 2 cut(s) 400, 452
Cfr13I GGNCC 4 cut(s) 19, 37, 572, 613
CpoI CGGWCCG 1 cut(s) 572
CseI GACGC 2 cut(s) 676, 716
Csp6I GTAC 1 cut(s) 478
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CspI CGGWCCG 1 cut(s) 572
CviAII CATG 8 cut(s) 58, 134, 163, 399, 431, 451, 598, 610
CviQI GTAC 1 cut(s) 478
DdeI CTNAG 4 cut(s) 197, 257, 527, 703
DpnI GATC 5 cut(s) 27, 281, 446, 677, 701
DpnII GATC 5 cut(s) 25, 279, 444, 675, 699
Eco130I CCWWGG 1 cut(s) 597
Eco47I GGWCC 4 cut(s) 19, 37, 572, 613
EcoO109I RGGNCCY 1 cut(s) 19
EcoRII CCWGG 1 cut(s) 638
EcoT14I CCWWGG 1 cut(s) 597
ErhI CCWWGG 1 cut(s) 597
FaeI CATG 8 cut(s) 61, 137, 166, 402, 434, 454, 601, 613
FaqI GGGAC 1 cut(s) 23
FatI CATG 8 cut(s) 57, 133, 162, 398, 430, 450, 597, 609
FbaI TGATCA 1 cut(s) 444
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 2 cut(s) 62, 196
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 1 cut(s) 389
GluI GCNGC 1 cut(s) 225
HaeIII GGCC 1 cut(s) 643
HapII CCGG 3 cut(s) 455, 470, 666
HgaI GACGC 2 cut(s) 676, 716
Hin1II CATG 8 cut(s) 61, 137, 166, 402, 434, 454, 601, 613
HinfI GANTC 3 cut(s) 159, 295, 577
HpaII CCGG 3 cut(s) 455, 470, 666
HphI GGTGA 1 cut(s) 518
Hpy188I TCNGA 7 cut(s) 272, 284, 300, 444, 576, 692, 706
Hpy188III TCNNGA 1 cut(s) 310
Hpy99I CGWCG 1 cut(s) 692
HpyAV CCTTC 3 cut(s) 89, 395, 403
HpyCH4III ACNGT 3 cut(s) 115, 304, 461
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 4 cut(s) 166, 434, 484, 649
HpyF3I CTNAG 4 cut(s) 197, 257, 527, 703
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 8 cut(s) 61, 137, 166, 402, 434, 454, 601, 613
Ksp22I TGATCA 1 cut(s) 444
Kzo9I GATC 5 cut(s) 25, 279, 444, 675, 699
LmnI GCTCC 2 cut(s) 266, 272
Lsp1109I GCAGC 1 cut(s) 236
MaeI CTAG 1 cut(s) 389
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 3 cut(s) 461, 516, 715
MalI GATC 5 cut(s) 27, 281, 446, 677, 701
MboI GATC 5 cut(s) 25, 279, 444, 675, 699
MboII GAAGA 1 cut(s) 298
MflI RGATCY 1 cut(s) 699
MhlI GDGCHC 1 cut(s) 535
MluCI AATT 3 cut(s) 289, 317, 321
MluI ACGCGT 1 cut(s) 685
MlyI GAGTC 1 cut(s) 304
MmeI TCCRAC 2 cut(s) 237, 670
MnlI CCTC 9 cut(s) 10, 114, 275, 359, 380, 497, 604, 626, 700
MroXI GAANNNNTTC 1 cut(s) 289
MseI TTAA 3 cut(s) 251, 320, 374
MspI CCGG 3 cut(s) 455, 470, 666
MspR9I CCNGG 2 cut(s) 640, 666
MvaI CCWGG 1 cut(s) 640
MvnI CGCG 1 cut(s) 687
NciI CCSGG 1 cut(s) 666
NcoI CCATGG 1 cut(s) 597
NdeII GATC 5 cut(s) 25, 279, 444, 675, 699
NlaIII CATG 8 cut(s) 61, 137, 166, 402, 434, 454, 601, 613
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 516, 715
NspI RCATGY 2 cut(s) 402, 454
PaeI GCATGC 2 cut(s) 402, 454
PdmI GAANNNNTTC 1 cut(s) 289
PfeI GAWTC 2 cut(s) 159, 577
PfoI TCCNGGA 1 cut(s) 664
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
Ppu21I YACGTR 1 cut(s) 481
PpuMI RGGWCCY 1 cut(s) 19
PshBI ATTAAT 1 cut(s) 320
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 638
PspGI CCWGG 1 cut(s) 638
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 4 cut(s) 19, 37, 572, 613
PspPPI RGGWCCY 1 cut(s) 19
PsuI RGATCY 1 cut(s) 699
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
Rsr2I CGGWCCG 1 cut(s) 572
RsrII CGGWCCG 1 cut(s) 572
SaqAI TTAA 3 cut(s) 251, 320, 374
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 5 cut(s) 25, 279, 444, 675, 699
Sau96I GGNCC 4 cut(s) 19, 37, 572, 613
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 2 cut(s) 640, 666
SduI GDGCHC 1 cut(s) 535
SfcI CTRYAG 1 cut(s) 85
SinI GGWCC 4 cut(s) 19, 37, 572, 613
SphI GCATGC 2 cut(s) 402, 454
Sse9I AATT 3 cut(s) 289, 317, 321
SsiI CCGC 1 cut(s) 491
SspMI CTAG 1 cut(s) 389
StyD4I CCNGG 2 cut(s) 638, 664
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 3 cut(s) 115, 304, 461
TaiI ACGT 1 cut(s) 483
TaqI TCGA 1 cut(s) 293
TaqII GACCGA 1 cut(s) 560
TasI AATT 3 cut(s) 289, 317, 321
TfiI GAWTC 2 cut(s) 159, 577
Tru1I TTAA 3 cut(s) 251, 320, 374
Tru9I TTAA 3 cut(s) 251, 320, 374
TscAI CASTG 1 cut(s) 651
TseFI GTSAC 2 cut(s) 516, 715
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 2 cut(s) 516, 715
TspDTI ATGAA 2 cut(s) 198, 371
TspRI CASTG 1 cut(s) 651
VpaK11BI GGWCC 4 cut(s) 19, 37, 572, 613
VspI ATTAAT 1 cut(s) 320
XceI RCATGY 2 cut(s) 402, 454
XmnI GAANNNNTTC 1 cut(s) 289
XspI CTAG 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.