Rw5G020420

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004799
Physical Location & Seq
Forward (+)
26925628 .. 26926549
922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G020420.1

Sequence Viewer

Length: 834 bp
ATGGTTTGGAAGAAGGAATACTTGGATTTAATCTTGGTCCCAACTGGGTTGCTAATCATGTTATGCTACCATCTCTTTCTCCTCTACAGATGCCTAAGGCATCCTGAAACCACAGTCATTGGCAATGAAAACCACTGTAGAAAAGCTTGGGTTGAGAGAATGTTGCAGATTGAAGCAAAGGATCGAGGTCAGTCCCTAGCAGTAATCTCCAGCACTATAACAGCAGCAAATTTCTTGGCTTCTACCTCTTTAGCCTTGAGTTCTTTGATTGGAACATGGGTTCTAAACTCATCAAACAGCATCCTCCTCACTAGTTTTACGTATGGTGACACAAGTCCTGCAATGAATTCTATTAAATACGTTAGCATTCTGGCCTGCTTCCTATTAGCTCTAGCTTCTTTCTTACACTGTATAAGAAGCTTTATCCATGCCACTTTCCTCATTAGCATGCCAAACAGTGATACTCCTCTGAGTGATGTTCAGAAGGCTGTGATACATGGCGGCCTTTTTTGGACATTGGGGTTGAGAGCAATCTATTTTGCCACTAATTTACTCCTCTGGGTTTTTGGTCCAATTCCGATGTTTGTGTGTTCAGTGACTTTGGTGTTGATTCTGAACAAGCTGGATTCGAATTCCACTCCATTGCATCAGTTCCAACCAGCCAGGAGTCAGGACAATAGGTTGATGGGGAAACTTGCTGAAGAAAAAACCCAAATAGATAGGGTTATCGAGCAACACGAAAGATCACACATAGATGATAACAGAACTCAAGGTTCTAACTGTCAACAATCACCTGACTCTGGTAAGGATTTAAGTTCAGAGGCAACAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

277

Amino Acids

30.91

Weight (kDa)

7.1

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 213 6.5e-58 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 501
AclWI GGATC 1 cut(s) 189
AcsI RAATTY 3 cut(s) 229, 346, 631
AcuI CTGAAG 1 cut(s) 720
AfiI CCNNNNNNNGG 1 cut(s) 800
AgsI TTSAA 1 cut(s) 173
AhlI ACTAGT 1 cut(s) 311
AjnI CCWGG 1 cut(s) 662
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AloI GAACNNNNNNTCC 2 cut(s) 264, 296
AluBI AGCT 5 cut(s) 146, 389, 395, 420, 622
AluI AGCT 5 cut(s) 146, 389, 395, 420, 622
AlwI GGATC 1 cut(s) 189
AoxI GGCC 2 cut(s) 372, 502
ApeKI GCWGC 1 cut(s) 224
ApoI RAATTY 3 cut(s) 229, 346, 631
AspS9I GGNCC 2 cut(s) 37, 569
AsuHPI GGTGA 2 cut(s) 338, 783
AsuII TTCGAA 1 cut(s) 629
AvaII GGWCC 2 cut(s) 37, 569
AxyI CCTNAGG 1 cut(s) 95
BarI GAAGNNNNNNTAC 1 cut(s) 34
BbvI GCAGC 1 cut(s) 236
BccI CCATC 2 cut(s) 78, 679
BciT130I CCWGG 1 cut(s) 664
BcuI ACTAGT 1 cut(s) 311
BfaI CTAG 3 cut(s) 197, 312, 392
BfmI CTRYAG 2 cut(s) 85, 136
BisI GCNGC 2 cut(s) 225, 502
BlsI GCNGC 2 cut(s) 226, 503
Bme1390I CCNGG 1 cut(s) 664
Bme18I GGWCC 2 cut(s) 37, 569
BmgT120I GGNCC 2 cut(s) 37, 569
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 664
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 4 cut(s) 80, 109, 309, 655
BmuI ACTGGG 1 cut(s) 54
BoxI GACNNNNGTC 1 cut(s) 333
BpmI CTGGAG 1 cut(s) 193
Bpu14I TTCGAA 1 cut(s) 629
BpuEI CTTGAG 2 cut(s) 277, 753
BsaAI YACGTR 1 cut(s) 321
Bsc4I CCNNNNNNNGG 1 cut(s) 800
Bse1I ACTGG 1 cut(s) 49
Bse21I CCTNAGG 1 cut(s) 95
Bse3DI GCAATG 3 cut(s) 130, 348, 641
BseBI CCWGG 1 cut(s) 664
BseGI GGATG 2 cut(s) 100, 300
BseLI CCNNNNNNNGG 1 cut(s) 800
BseMI GCAATG 3 cut(s) 130, 348, 641
BseMII CTCAG 1 cut(s) 461
BseNI ACTGG 1 cut(s) 49
BseRI GAGGAG 4 cut(s) 71, 296, 456, 545
BseXI GCAGC 1 cut(s) 236
BshFI GGCC 2 cut(s) 374, 504
BslFI GGGAC 2 cut(s) 23, 178
BslI CCNNNNNNNGG 1 cut(s) 800
BsmFI GGGAC 2 cut(s) 23, 178
BsmI GAATGC 1 cut(s) 366
BsnI GGCC 2 cut(s) 374, 504
Bsp119I TTCGAA 1 cut(s) 629
Bsp143I GATC 2 cut(s) 181, 743
BspACI CCGC 1 cut(s) 501
BspANI GGCC 2 cut(s) 374, 504
BspCNI CTCAG 1 cut(s) 462
BspLI GGNNCC 1 cut(s) 39
BspPI GGATC 1 cut(s) 189
BspT104I TTCGAA 1 cut(s) 629
BsrDI GCAATG 3 cut(s) 130, 348, 641
BsrI ACTGG 1 cut(s) 49
BssMI GATC 2 cut(s) 181, 743
Bst2UI CCWGG 1 cut(s) 664
Bst4CI ACNGT 5 cut(s) 115, 137, 410, 458, 782
BstBAI YACGTR 1 cut(s) 321
BstBI TTCGAA 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 376, 449
BstDEI CTNAG 2 cut(s) 95, 470
BstF5I GGATG 2 cut(s) 100, 300
BstKTI GATC 2 cut(s) 184, 746
BstMBI GATC 2 cut(s) 181, 743
BstNI CCWGG 1 cut(s) 664
BstNSI RCATGY 1 cut(s) 451
BstPAI GACNNNNGTC 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 662
BstSFI CTRYAG 2 cut(s) 85, 136
BstSNI TACGTA 1 cut(s) 321
BstV1I GCAGC 1 cut(s) 236
Bsu36I CCTNAGG 1 cut(s) 95
BsuRI GGCC 2 cut(s) 374, 504
BtsCI GGATG 2 cut(s) 100, 300
BtsIMutI CAGTG 4 cut(s) 133, 406, 463, 600
Cac8I GCNNGC 2 cut(s) 376, 449
Cfr13I GGNCC 2 cut(s) 37, 569
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 5 cut(s) 58, 276, 428, 448, 497
DdeI CTNAG 2 cut(s) 95, 470
DpnI GATC 2 cut(s) 183, 745
DpnII GATC 2 cut(s) 181, 743
Eco105I TACGTA 1 cut(s) 321
Eco47I GGWCC 2 cut(s) 37, 569
Eco57I CTGAAG 1 cut(s) 720
Eco81I CCTNAGG 1 cut(s) 95
EcoRI GAATTC 2 cut(s) 346, 631
EcoRII CCWGG 1 cut(s) 662
FaeI CATG 5 cut(s) 61, 279, 431, 451, 500
FalI AAGNNNNNCTT 1 cut(s) 37
FaqI GGGAC 2 cut(s) 23, 178
FatI CATG 5 cut(s) 57, 275, 427, 447, 496
Fnu4HI GCNGC 2 cut(s) 225, 502
FokI GGATG 2 cut(s) 87, 287
Fsp4HI GCNGC 2 cut(s) 225, 502
FspBI CTAG 3 cut(s) 197, 312, 392
GluI GCNGC 2 cut(s) 225, 502
GsuI CTGGAG 1 cut(s) 193
HaeIII GGCC 2 cut(s) 374, 504
Hin1II CATG 5 cut(s) 61, 279, 431, 451, 500
HincII GTYRAC 1 cut(s) 785
HindII GTYRAC 1 cut(s) 785
HindIII AAGCTT 2 cut(s) 144, 418
HinfI GANTC 4 cut(s) 610, 626, 667, 797
HphI GGTGA 2 cut(s) 338, 783
Hpy166II GTNNAC 1 cut(s) 785
Hpy188I TCNGA 5 cut(s) 471, 483, 579, 615, 820
Hpy188III TCNNGA 2 cut(s) 104, 671
Hpy8I GTNNAC 1 cut(s) 785
HpyAV CCTTC 2 cut(s) 7, 478
HpyCH4III ACNGT 5 cut(s) 115, 137, 410, 458, 782
HpyCH4IV ACGT 2 cut(s) 320, 360
HpyCH4V TGCA 3 cut(s) 166, 341, 646
HpyF3I CTNAG 2 cut(s) 95, 470
HpySE526I ACGT 2 cut(s) 320, 360
Hsp92II CATG 5 cut(s) 61, 279, 431, 451, 500
Kzo9I GATC 2 cut(s) 181, 743
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 4 cut(s) 80, 109, 309, 655
MaeI CTAG 3 cut(s) 197, 312, 392
MaeII ACGT 2 cut(s) 320, 360
MaeIII GTNAC 2 cut(s) 326, 595
MalI GATC 2 cut(s) 183, 745
MboI GATC 2 cut(s) 181, 743
MboII GAAGA 2 cut(s) 22, 713
MluCI AATT 5 cut(s) 229, 346, 547, 573, 631
MlyI GAGTC 2 cut(s) 676, 791
MmeI TCCRAC 1 cut(s) 679
MnlI CCTC 9 cut(s) 92, 179, 256, 314, 317, 449, 477, 566, 814
MseI TTAA 4 cut(s) 29, 354, 812, 832
MslI CAYNNNNRTG 2 cut(s) 446, 753
MspR9I CCNGG 1 cut(s) 664
Mva1269I GAATGC 1 cut(s) 366
MvaI CCWGG 1 cut(s) 664
NdeII GATC 2 cut(s) 181, 743
NlaIII CATG 5 cut(s) 61, 279, 431, 451, 500
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 326, 595
NspI RCATGY 1 cut(s) 451
NspV TTCGAA 1 cut(s) 629
PaeI GCATGC 1 cut(s) 451
PcsI WCGNNNNNNNCGW 1 cut(s) 735
PctI GAATGC 1 cut(s) 366
PfeI GAWTC 2 cut(s) 610, 626
PkrI GCNGC 2 cut(s) 226, 503
PleI GAGTC 2 cut(s) 675, 791
PpsI GAGTC 2 cut(s) 675, 791
Ppu21I YACGTR 1 cut(s) 321
PshAI GACNNNNGTC 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 662
PspGI CCWGG 1 cut(s) 662
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 2 cut(s) 37, 569
RseI CAYNNNNRTG 2 cut(s) 446, 753
SaqAI TTAA 4 cut(s) 29, 354, 812, 832
SatI GCNGC 2 cut(s) 225, 502
Sau3AI GATC 2 cut(s) 181, 743
Sau96I GGNCC 2 cut(s) 37, 569
SchI GAGTC 2 cut(s) 676, 791
ScrFI CCNGG 1 cut(s) 664
SfaNI GCATC 4 cut(s) 80, 109, 309, 655
SfcI CTRYAG 2 cut(s) 85, 136
SfuI TTCGAA 1 cut(s) 629
SinI GGWCC 2 cut(s) 37, 569
SmiMI CAYNNNNRTG 2 cut(s) 446, 753
SmlI CTYRAG 2 cut(s) 256, 768
SmoI CTYRAG 2 cut(s) 256, 768
SnaBI TACGTA 1 cut(s) 321
SpeI ACTAGT 1 cut(s) 311
SphI GCATGC 1 cut(s) 451
Sse9I AATT 5 cut(s) 229, 346, 547, 573, 631
SsiI CCGC 1 cut(s) 501
SspMI CTAG 3 cut(s) 197, 312, 392
StyD4I CCNGG 1 cut(s) 662
TaaI ACNGT 5 cut(s) 115, 137, 410, 458, 782
TaiI ACGT 2 cut(s) 323, 363
TaqI TCGA 3 cut(s) 184, 629, 729
TasI AATT 5 cut(s) 229, 346, 547, 573, 631
TauI GCSGC 1 cut(s) 504
TfiI GAWTC 2 cut(s) 610, 626
Tru1I TTAA 4 cut(s) 29, 354, 812, 832
Tru9I TTAA 4 cut(s) 29, 354, 812, 832
TscAI CASTG 4 cut(s) 140, 413, 463, 600
TseFI GTSAC 2 cut(s) 326, 595
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 2 cut(s) 326, 595
TspDTI ATGAA 2 cut(s) 141, 359
TspRI CASTG 4 cut(s) 140, 413, 463, 600
VpaK11BI GGWCC 2 cut(s) 37, 569
XapI RAATTY 3 cut(s) 229, 346, 631
XceI RCATGY 1 cut(s) 451
XspI CTAG 3 cut(s) 197, 312, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.