MD11G1256800.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004799
Physical Location & Seq
Reverse (-)
36917440 .. 36918929
1490 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1256800.v1.1.491

Sequence Viewer

Length: 660 bp
ATGGTTTGGAAGAAGGAATACTTAGATTTAATCTTGGTCCCAACTGGGTTGCTAATCATGCTTTGCTACCATCTTTTTCTTCTGTATAGATACCTAAGACATCCTGAAACTACAGTCATTGGCAATGAAAACCACTGCAGAAAAGCTTGGGTTGAGAGAATGTTGCAGGTTGATGCAAAGGACAGAGGTATGTCCCTAGCAGTAATCTCTAGCACCACAACAGCAGCAAATTTCTTAGCCTCAACCTCTCTAGCCTTAAGCTCTCTCATCGGAGCATGGATTGGAAACTCTTCGCACAATGTCTTCACGAGCAGTATTACATACGGCGACACAAGTCCTATGCTAGTTTCCGTCAAATATATTAGCATTCTGGCCTGCTTCCTGCTGGCCTTAGCTTCTTTTTTACATTGTATAAGAGACTTTGTTCATGCCAATTTCCTCATCAGTATGCCAGACAGTGATATTCCTCAGGAGCATGTCGAGAAGGCGGTGATAAGTGGAGGCTTGTTCTGGACATTGGGGCTAAGAGCTATCTACTTTGCCACTACTGTGCTGCTTTGGATTTTTGGTCCGATTCCAATGTTTGTTTGCTCAGTGACCATGGTGTTGGTTTTGCACAGGCTTGATTCAAATTCCACTCCTTTAAATAAGTTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

220

Amino Acids

24.5

Weight (kDa)

8.28

Isoelectric Point (pI)

25.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 213 1.1e-63 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 157
AciI CCGC 1 cut(s) 488
AcsI RAATTY 2 cut(s) 229, 631
AflII CTTAAG 1 cut(s) 256
AgsI TTSAA 2 cut(s) 630, 655
AleI CACNNNNGTG 1 cut(s) 548
AluBI AGCT 4 cut(s) 146, 261, 395, 530
AluI AGCT 4 cut(s) 146, 261, 395, 530
Alw26I GTCTC 1 cut(s) 411
AoxI GGCC 2 cut(s) 372, 387
ApeKI GCWGC 2 cut(s) 224, 553
ApoI RAATTY 2 cut(s) 229, 631
Asp700I GAANNNNTTC 1 cut(s) 289
AspS9I GGNCC 2 cut(s) 37, 569
AsuHPI GGTGA 1 cut(s) 502
AvaII GGWCC 2 cut(s) 37, 569
AxyI CCTNAGG 1 cut(s) 468
BarI GAAGNNNNNNTAC 1 cut(s) 34
BauI CACGAG 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 295
BbvI GCAGC 2 cut(s) 236, 540
BccI CCATC 1 cut(s) 78
BceAI ACGGC 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 411
BfaI CTAG 4 cut(s) 197, 210, 251, 344
BfmI CTRYAG 2 cut(s) 111, 136
BfrI CTTAAG 1 cut(s) 256
BfuAI ACCTGC 1 cut(s) 157
BisI GCNGC 2 cut(s) 225, 554
BlsI GCNGC 2 cut(s) 226, 555
Bme18I GGWCC 2 cut(s) 37, 569
BmgT120I GGNCC 2 cut(s) 37, 569
BmiI GGNNCC 1 cut(s) 39
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 1 cut(s) 163
BmuI ACTGGG 1 cut(s) 54
BoxI GACNNNNGTC 1 cut(s) 333
BpiI GAAGAC 1 cut(s) 295
Bpu10I CCTNAGC 1 cut(s) 391
BsaJI CCNNGG 1 cut(s) 600
Bse1I ACTGG 1 cut(s) 49
Bse21I CCTNAGG 1 cut(s) 468
Bse3DI GCAATG 1 cut(s) 130
BseDI CCNNGG 1 cut(s) 600
BseGI GGATG 1 cut(s) 100
BseMI GCAATG 1 cut(s) 130
BseMII CTCAG 2 cut(s) 482, 606
BseNI ACTGG 1 cut(s) 49
BseXI GCAGC 2 cut(s) 236, 540
BshFI GGCC 2 cut(s) 374, 389
BslFI GGGAC 2 cut(s) 23, 178
BsmAI GTCTC 1 cut(s) 411
BsmFI GGGAC 2 cut(s) 23, 178
BsmI GAATGC 1 cut(s) 366
BsnI GGCC 2 cut(s) 374, 389
Bsp19I CCATGG 1 cut(s) 600
BspACI CCGC 1 cut(s) 488
BspANI GGCC 2 cut(s) 374, 389
BspCNI CTCAG 2 cut(s) 481, 605
BspLI GGNNCC 1 cut(s) 39
BspMAI CTGCAG 1 cut(s) 140
BspMI ACCTGC 1 cut(s) 157
BspTI CTTAAG 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 130
BsrI ACTGG 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 600
BssSI CACGAG 1 cut(s) 307
BssT1I CCWWGG 1 cut(s) 600
Bst2BI CACGAG 1 cut(s) 307
Bst4CI ACNGT 3 cut(s) 115, 458, 550
Bst6I CTCTTC 1 cut(s) 295
BstAFI CTTAAG 1 cut(s) 256
BstC8I GCNNGC 2 cut(s) 376, 387
BstDEI CTNAG 7 cut(s) 22, 95, 235, 391, 468, 524, 592
BstDSI CCRYGG 1 cut(s) 600
BstF5I GGATG 1 cut(s) 100
BstMAI GTCTC 1 cut(s) 411
BstMWI GCNNNNNNNGC 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 479
BstPAI GACNNNNGTC 1 cut(s) 333
BstSFI CTRYAG 2 cut(s) 111, 136
BstV1I GCAGC 2 cut(s) 236, 540
BstV2I GAAGAC 1 cut(s) 295
BstXI CCANNNNNNTGG 1 cut(s) 607
Bsu36I CCTNAGG 1 cut(s) 468
BsuRI GGCC 2 cut(s) 374, 389
BtgI CCRYGG 1 cut(s) 600
BtsCI GGATG 1 cut(s) 100
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 3 cut(s) 133, 463, 600
BveI ACCTGC 1 cut(s) 157
Cac8I GCNNGC 2 cut(s) 376, 387
Cfr13I GGNCC 2 cut(s) 37, 569
CviAII CATG 5 cut(s) 58, 276, 428, 476, 601
DdeI CTNAG 7 cut(s) 22, 95, 235, 391, 468, 524, 592
DraI TTTAAA 1 cut(s) 645
Eam1104I CTCTTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 600
Eco47I GGWCC 2 cut(s) 37, 569
Eco81I CCTNAGG 1 cut(s) 468
EcoT14I CCWWGG 1 cut(s) 600
ErhI CCWWGG 1 cut(s) 600
FaeI CATG 5 cut(s) 61, 279, 431, 479, 604
FalI AAGNNNNNCTT 1 cut(s) 37
FaqI GGGAC 2 cut(s) 23, 178
FatI CATG 5 cut(s) 57, 275, 427, 475, 600
Fnu4HI GCNGC 2 cut(s) 225, 554
FokI GGATG 1 cut(s) 87
Fsp4HI GCNGC 2 cut(s) 225, 554
FspBI CTAG 4 cut(s) 197, 210, 251, 344
GluI GCNGC 2 cut(s) 225, 554
HaeIII GGCC 2 cut(s) 374, 389
Hin1II CATG 5 cut(s) 61, 279, 431, 479, 604
HindIII AAGCTT 1 cut(s) 144
HinfI GANTC 2 cut(s) 574, 626
HphI GGTGA 1 cut(s) 502
Hpy188I TCNGA 2 cut(s) 272, 573
Hpy188III TCNNGA 5 cut(s) 104, 307, 470, 481, 511
HpyAV CCTTC 2 cut(s) 7, 478
HpyCH4III ACNGT 3 cut(s) 115, 458, 550
HpyCH4V TGCA 4 cut(s) 138, 166, 176, 616
HpyF10VI GCNNNNNNNGC 1 cut(s) 58
HpyF3I CTNAG 7 cut(s) 22, 95, 235, 391, 468, 524, 592
Hsp92II CATG 5 cut(s) 61, 279, 431, 479, 604
LmnI GCTCC 2 cut(s) 272, 472
Lsp1109I GCAGC 2 cut(s) 236, 540
LweI GCATC 1 cut(s) 163
MaeI CTAG 4 cut(s) 197, 210, 251, 344
MaeIII GTNAC 1 cut(s) 595
MboII GAAGA 4 cut(s) 22, 71, 282, 295
MluCI AATT 3 cut(s) 229, 433, 631
MnlI CCTC 6 cut(s) 179, 250, 256, 449, 477, 494
MroXI GAANNNNTTC 1 cut(s) 289
MseI TTAA 3 cut(s) 29, 257, 644
MslI CAYNNNNRTG 2 cut(s) 446, 548
MspCI CTTAAG 1 cut(s) 256
Mva1269I GAATGC 1 cut(s) 366
MwoI GCNNNNNNNGC 1 cut(s) 58
NcoI CCATGG 1 cut(s) 600
NlaIII CATG 5 cut(s) 61, 279, 431, 479, 604
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 1 cut(s) 595
NspI RCATGY 1 cut(s) 479
OliI CACNNNNGTG 1 cut(s) 548
PctI GAATGC 1 cut(s) 366
PdmI GAANNNNTTC 1 cut(s) 289
PfeI GAWTC 2 cut(s) 574, 626
PkrI GCNGC 2 cut(s) 226, 555
PshAI GACNNNNGTC 1 cut(s) 333
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 2 cut(s) 37, 569
PstI CTGCAG 1 cut(s) 140
RseI CAYNNNNRTG 2 cut(s) 446, 548
SaqAI TTAA 3 cut(s) 29, 257, 644
SatI GCNGC 2 cut(s) 225, 554
Sau96I GGNCC 2 cut(s) 37, 569
SetI ASST 8 cut(s) 96, 148, 171, 190, 248, 263, 397, 532
SfaNI GCATC 1 cut(s) 163
SfcI CTRYAG 2 cut(s) 111, 136
SinI GGWCC 2 cut(s) 37, 569
SmiMI CAYNNNNRTG 2 cut(s) 446, 548
SmlI CTYRAG 1 cut(s) 256
SmoI CTYRAG 1 cut(s) 256
Sse9I AATT 3 cut(s) 229, 433, 631
SsiI CCGC 1 cut(s) 488
SspMI CTAG 4 cut(s) 197, 210, 251, 344
StyI CCWWGG 1 cut(s) 600
TaaI ACNGT 3 cut(s) 115, 458, 550
TaqI TCGA 1 cut(s) 480
TasI AATT 3 cut(s) 229, 433, 631
TfiI GAWTC 2 cut(s) 574, 626
Tru1I TTAA 3 cut(s) 29, 257, 644
Tru9I TTAA 3 cut(s) 29, 257, 644
TscAI CASTG 3 cut(s) 140, 463, 600
TseFI GTSAC 1 cut(s) 595
TseI GCWGC 2 cut(s) 224, 553
Tsp45I GTSAC 1 cut(s) 595
TspDTI ATGAA 2 cut(s) 141, 416
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 3 cut(s) 140, 463, 600
Vha464I CTTAAG 1 cut(s) 256
VpaK11BI GGWCC 2 cut(s) 37, 569
XapI RAATTY 2 cut(s) 229, 631
XceI RCATGY 1 cut(s) 479
XmnI GAANNNNTTC 1 cut(s) 289
XspI CTAG 4 cut(s) 197, 210, 251, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.