Rh5BG224700

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004799
Physical Location & Seq
Forward (+)
26135997 .. 26137307
1311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG224700.1

Sequence Viewer

Length: 834 bp
ATGGTTTGGAAGAAGGAATACTTGGATTTAATCTTGGTCCCAACTGGGTTGCTAATCATATGCCTAAGGCATCCTGAAACCACAGTCATTGGCAATGAAAACCACTGTAGAAAAGCTTGGGTTGAGAGAATGTTGCAGATTGAAGCAAAGGATCGAGGTCAGTCCCTAGCAGTAATCTCCAGCACTATAACAGCAGCAAATTTCTTGGCTTCAACCTCTTTAGCCTTGAGTTCTTTGATTGGAACATGGGTTCTAAACTCTTCAAACAGCATCCTCCTCACTAGTTTTACGTATGGTGACACAAGTCCTGCAATGAATTCTATTAAATACGTTAGCATTCTGGCCTGCTTCCTATTAGCTCTAGCTTCTTTCTTACACTGTATAAGAAGCTTTATCCATGCCACTTTCCTCATGAGCATGCCAAACAGTGATACTCCTCTGAGTGATGTTCAGAAGGCTGTGATACATGGCGGCCTTTTTTGGACATTGGGGTTGAGAGCAATCTATTTTGCCACTAATTTACTCCTCTGGGTTTTTGGTCCAATTCCGATGTTTGTGTGTTCAGTGACTTTGGTGTTGATTCTGAACAAGCTGGATTCGAATTCCACACCATTGCATCAGTTCCAACCAGCCAGGAGTCAGGACAATAGGTTGATGGGGAAACTTGCTGAAGAAAAAACCCAAATAGATAGGGTTATCGAGCAACACGAAAGATCACACATAGATGATAACAGAACTCAAGGTTCTAACTGTCAACAATCACCTGCCTCTGGTTTGCAGAAAGCACAAAATTATGGGCAGCTTCTTCAGAGGCAGAAGCCCTTTTCTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

277

Amino Acids

30.87

Weight (kDa)

8.75

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 19 - 203 1.1e-49 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 772
Acc36I ACCTGC 1 cut(s) 772
AciI CCGC 1 cut(s) 471
AclWI GGATC 1 cut(s) 159
AcsI RAATTY 3 cut(s) 199, 316, 601
AcuI CTGAAG 2 cut(s) 690, 791
AfiI CCNNNNNNNGG 1 cut(s) 770
AgsI TTSAA 3 cut(s) 143, 213, 264
AhlI ACTAGT 1 cut(s) 281
AjnI CCWGG 1 cut(s) 632
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AloI GAACNNNNNNTCC 2 cut(s) 234, 266
AluBI AGCT 6 cut(s) 116, 359, 365, 390, 592, 802
AluI AGCT 6 cut(s) 116, 359, 365, 390, 592, 802
AlwI GGATC 1 cut(s) 159
AoxI GGCC 2 cut(s) 342, 472
ApeKI GCWGC 2 cut(s) 194, 799
ApoI RAATTY 3 cut(s) 199, 316, 601
AspS9I GGNCC 2 cut(s) 37, 539
AsuHPI GGTGA 2 cut(s) 308, 753
AsuII TTCGAA 1 cut(s) 599
AvaII GGWCC 2 cut(s) 37, 539
AxyI CCTNAGG 1 cut(s) 65
BarI GAAGNNNNNNTAC 1 cut(s) 34
BbvI GCAGC 2 cut(s) 206, 811
BccI CCATC 1 cut(s) 649
BciT130I CCWGG 1 cut(s) 634
BcuI ACTAGT 1 cut(s) 281
BfaI CTAG 3 cut(s) 167, 282, 362
BfmI CTRYAG 1 cut(s) 106
BfuAI ACCTGC 1 cut(s) 772
BisI GCNGC 3 cut(s) 195, 472, 800
BlsI GCNGC 3 cut(s) 196, 473, 801
Bme1390I CCNGG 1 cut(s) 634
Bme18I GGWCC 2 cut(s) 37, 539
BmgT120I GGNCC 2 cut(s) 37, 539
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 634
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 3 cut(s) 79, 279, 625
BmuI ACTGGG 1 cut(s) 54
BoxI GACNNNNGTC 1 cut(s) 303
BpmI CTGGAG 1 cut(s) 163
Bpu14I TTCGAA 1 cut(s) 599
BpuEI CTTGAG 2 cut(s) 247, 723
BsaAI YACGTR 1 cut(s) 291
Bsc4I CCNNNNNNNGG 1 cut(s) 770
Bse1I ACTGG 1 cut(s) 49
Bse21I CCTNAGG 1 cut(s) 65
Bse3DI GCAATG 3 cut(s) 100, 318, 611
BseBI CCWGG 1 cut(s) 634
BseGI GGATG 2 cut(s) 70, 270
BseLI CCNNNNNNNGG 1 cut(s) 770
BseMI GCAATG 3 cut(s) 100, 318, 611
BseMII CTCAG 1 cut(s) 431
BseNI ACTGG 1 cut(s) 49
BseRI GAGGAG 3 cut(s) 266, 426, 515
BseXI GCAGC 2 cut(s) 206, 811
BshFI GGCC 2 cut(s) 344, 474
BslFI GGGAC 2 cut(s) 23, 148
BslI CCNNNNNNNGG 1 cut(s) 770
BsmFI GGGAC 2 cut(s) 23, 148
BsmI GAATGC 1 cut(s) 336
BsnI GGCC 2 cut(s) 344, 474
Bsp119I TTCGAA 1 cut(s) 599
Bsp143I GATC 2 cut(s) 151, 713
BspACI CCGC 1 cut(s) 471
BspANI GGCC 2 cut(s) 344, 474
BspCNI CTCAG 1 cut(s) 432
BspHI TCATGA 1 cut(s) 411
BspLI GGNNCC 1 cut(s) 39
BspMI ACCTGC 1 cut(s) 772
BspPI GGATC 1 cut(s) 159
BspT104I TTCGAA 1 cut(s) 599
BsrDI GCAATG 3 cut(s) 100, 318, 611
BsrI ACTGG 1 cut(s) 49
BssMI GATC 2 cut(s) 151, 713
Bst2UI CCWGG 1 cut(s) 634
Bst4CI ACNGT 5 cut(s) 85, 107, 380, 428, 752
Bst6I CTCTTC 1 cut(s) 265
BstBAI YACGTR 1 cut(s) 291
BstBI TTCGAA 1 cut(s) 599
BstC8I GCNNGC 2 cut(s) 346, 419
BstDEI CTNAG 2 cut(s) 65, 440
BstF5I GGATG 2 cut(s) 70, 270
BstKTI GATC 2 cut(s) 154, 716
BstMBI GATC 2 cut(s) 151, 713
BstNI CCWGG 1 cut(s) 634
BstNSI RCATGY 1 cut(s) 421
BstPAI GACNNNNGTC 1 cut(s) 303
BstSCI CCNGG 1 cut(s) 632
BstSFI CTRYAG 1 cut(s) 106
BstSNI TACGTA 1 cut(s) 291
BstV1I GCAGC 2 cut(s) 206, 811
Bsu36I CCTNAGG 1 cut(s) 65
BsuRI GGCC 2 cut(s) 344, 474
BtsCI GGATG 2 cut(s) 70, 270
BtsIMutI CAGTG 4 cut(s) 103, 376, 433, 570
BveI ACCTGC 1 cut(s) 772
Cac8I GCNNGC 2 cut(s) 346, 419
CciI TCATGA 1 cut(s) 411
Cfr13I GGNCC 2 cut(s) 37, 539
CspCI CAANNNNNGTGG 2 cut(s) 70, 105
CviAII CATG 5 cut(s) 246, 398, 412, 418, 467
DdeI CTNAG 2 cut(s) 65, 440
DpnI GATC 2 cut(s) 153, 715
DpnII GATC 2 cut(s) 151, 713
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
Eco105I TACGTA 1 cut(s) 291
Eco47I GGWCC 2 cut(s) 37, 539
Eco57I CTGAAG 2 cut(s) 690, 791
Eco81I CCTNAGG 1 cut(s) 65
EcoRI GAATTC 2 cut(s) 316, 601
EcoRII CCWGG 1 cut(s) 632
FaeI CATG 5 cut(s) 249, 401, 415, 421, 470
FalI AAGNNNNNCTT 1 cut(s) 37
FaqI GGGAC 2 cut(s) 23, 148
FatI CATG 5 cut(s) 245, 397, 411, 417, 466
FauNDI CATATG 1 cut(s) 59
Fnu4HI GCNGC 3 cut(s) 195, 472, 800
FokI GGATG 2 cut(s) 57, 257
Fsp4HI GCNGC 3 cut(s) 195, 472, 800
FspBI CTAG 3 cut(s) 167, 282, 362
GluI GCNGC 3 cut(s) 195, 472, 800
GsuI CTGGAG 1 cut(s) 163
HaeIII GGCC 2 cut(s) 344, 474
Hin1II CATG 5 cut(s) 249, 401, 415, 421, 470
HincII GTYRAC 1 cut(s) 755
HindII GTYRAC 1 cut(s) 755
HindIII AAGCTT 2 cut(s) 114, 388
HinfI GANTC 3 cut(s) 580, 596, 637
HphI GGTGA 2 cut(s) 308, 753
Hpy166II GTNNAC 1 cut(s) 755
Hpy188I TCNGA 5 cut(s) 441, 453, 549, 585, 810
Hpy188III TCNNGA 3 cut(s) 74, 412, 641
Hpy8I GTNNAC 1 cut(s) 755
HpyAV CCTTC 2 cut(s) 7, 448
HpyCH4III ACNGT 5 cut(s) 85, 107, 380, 428, 752
HpyCH4IV ACGT 2 cut(s) 290, 330
HpyCH4V TGCA 4 cut(s) 136, 311, 616, 778
HpyF3I CTNAG 2 cut(s) 65, 440
HpySE526I ACGT 2 cut(s) 290, 330
Hsp92II CATG 5 cut(s) 249, 401, 415, 421, 470
Kzo9I GATC 2 cut(s) 151, 713
Lsp1109I GCAGC 2 cut(s) 206, 811
LweI GCATC 3 cut(s) 79, 279, 625
MaeI CTAG 3 cut(s) 167, 282, 362
MaeII ACGT 2 cut(s) 290, 330
MaeIII GTNAC 2 cut(s) 296, 565
MalI GATC 2 cut(s) 153, 715
MboI GATC 2 cut(s) 151, 713
MboII GAAGA 5 cut(s) 22, 252, 683, 797, 819
MluCI AATT 6 cut(s) 199, 316, 517, 543, 601, 790
MlyI GAGTC 1 cut(s) 646
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 9 cut(s) 149, 226, 284, 287, 419, 447, 536, 778, 804
MseI TTAA 2 cut(s) 29, 324
MslI CAYNNNNRTG 2 cut(s) 416, 723
MspR9I CCNGG 1 cut(s) 634
Mva1269I GAATGC 1 cut(s) 336
MvaI CCWGG 1 cut(s) 634
NdeI CATATG 1 cut(s) 59
NdeII GATC 2 cut(s) 151, 713
NlaIII CATG 5 cut(s) 249, 401, 415, 421, 470
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 296, 565
NspI RCATGY 1 cut(s) 421
NspV TTCGAA 1 cut(s) 599
PaeI GCATGC 1 cut(s) 421
PagI TCATGA 1 cut(s) 411
PaqCI CACCTGC 1 cut(s) 772
PcsI WCGNNNNNNNCGW 1 cut(s) 705
PctI GAATGC 1 cut(s) 336
PfeI GAWTC 2 cut(s) 580, 596
PkrI GCNGC 3 cut(s) 196, 473, 801
PleI GAGTC 1 cut(s) 645
PpsI GAGTC 1 cut(s) 645
Ppu21I YACGTR 1 cut(s) 291
PshAI GACNNNNGTC 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 632
PspGI CCWGG 1 cut(s) 632
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 2 cut(s) 37, 539
RseI CAYNNNNRTG 2 cut(s) 416, 723
SaqAI TTAA 2 cut(s) 29, 324
SatI GCNGC 3 cut(s) 195, 472, 800
Sau3AI GATC 2 cut(s) 151, 713
Sau96I GGNCC 2 cut(s) 37, 539
SchI GAGTC 1 cut(s) 646
ScrFI CCNGG 1 cut(s) 634
SfaNI GCATC 3 cut(s) 79, 279, 625
SfcI CTRYAG 1 cut(s) 106
SfuI TTCGAA 1 cut(s) 599
SinI GGWCC 2 cut(s) 37, 539
SmiMI CAYNNNNRTG 2 cut(s) 416, 723
SmlI CTYRAG 2 cut(s) 226, 738
SmoI CTYRAG 2 cut(s) 226, 738
SnaBI TACGTA 1 cut(s) 291
SpeI ACTAGT 1 cut(s) 281
SphI GCATGC 1 cut(s) 421
Sse9I AATT 6 cut(s) 199, 316, 517, 543, 601, 790
SsiI CCGC 1 cut(s) 471
SspMI CTAG 3 cut(s) 167, 282, 362
StyD4I CCNGG 1 cut(s) 632
TaaI ACNGT 5 cut(s) 85, 107, 380, 428, 752
TaiI ACGT 2 cut(s) 293, 333
TaqI TCGA 3 cut(s) 154, 599, 699
TasI AATT 6 cut(s) 199, 316, 517, 543, 601, 790
TauI GCSGC 1 cut(s) 474
TfiI GAWTC 2 cut(s) 580, 596
Tru1I TTAA 2 cut(s) 29, 324
Tru9I TTAA 2 cut(s) 29, 324
TscAI CASTG 4 cut(s) 110, 383, 433, 570
TseFI GTSAC 2 cut(s) 296, 565
TseI GCWGC 2 cut(s) 194, 799
Tsp45I GTSAC 2 cut(s) 296, 565
TspDTI ATGAA 3 cut(s) 111, 329, 819
TspRI CASTG 4 cut(s) 110, 383, 433, 570
VpaK11BI GGWCC 2 cut(s) 37, 539
XapI RAATTY 3 cut(s) 199, 316, 601
XceI RCATGY 1 cut(s) 421
XspI CTAG 3 cut(s) 167, 282, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.