AT5G52370

Mitochondrial 28S ribosomal protein S34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009129
Physical Location & Seq
Reverse (-)
21260278 .. 21261336
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52370.1

Sequence Viewer

Length: 429 bp
ATGGCGATGACACTTATTAACAGAGCAATCTCTAGAACCGAAACCGTTGGTGCTTTCAGACTCTCGCTTAATCTCTTAAGAAATTTCTCTGCGGCGCCGGCGAGTGAAAACCCTAGCTCCGATTCGAGTAAACCGAAACGAAGAAAGAAGAAGAACTTAATCGAAGTTGCTCAGTTCTTACCTAATTGGGGAATCGGATACCATATGGCTAAAGCTCATTGGAATGGAATCTCTTATGAAATCACTAAGATCAATCTCTATAAGGATGGTAGACATGGAAAAGCTTGGGGAATTGTTCACAAAGATGGATTGCGAGCTGCAGAAGCTCCAAAGAAGATAAGCGGAGTTCACAAACGGTGTTGGAAGTATATCCCAAACCTGTCAAAGACTGCTCCTGCTACAACCTCTGCTCATGTCCAAGCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S34

Protein Analysis

142

Amino Acids

15.76

Weight (kDa)

10.42

Isoelectric Point (pI)

39.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S34 PF16053 51 - 126 3.5e-08 Mitochondrial 28S ribosomal protein S34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 94
AccI GTMKAC 1 cut(s) 271
AciI CCGC 2 cut(s) 92, 342
AcsI RAATTY 1 cut(s) 82
AcyI GRCGYC 1 cut(s) 95
AfiI CCNNNNNNNGG 1 cut(s) 188
AflII CTTAAG 1 cut(s) 76
AluBI AGCT 6 cut(s) 117, 215, 284, 317, 326, 422
AluI AGCT 6 cut(s) 117, 215, 284, 317, 326, 422
ApeKI GCWGC 2 cut(s) 317, 422
ApoI RAATTY 1 cut(s) 82
AspLEI GCGC 1 cut(s) 97
BanI GGYRCC 1 cut(s) 94
BbvI GCAGC 2 cut(s) 304, 409
BccI CCATC 2 cut(s) 260, 299
BciVI GTATCC 1 cut(s) 191
BfaI CTAG 2 cut(s) 33, 114
BfmI CTRYAG 1 cut(s) 318
BfoI RGCGCY 1 cut(s) 98
BfrI CTTAAG 1 cut(s) 76
BfuI GTATCC 1 cut(s) 191
BisI GCNGC 3 cut(s) 93, 318, 423
BlsI GCNGC 3 cut(s) 94, 319, 424
BmiI GGNNCC 1 cut(s) 96
BsaHI GRCGYC 1 cut(s) 95
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse118I RCCGGY 1 cut(s) 97
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMII CTCAG 1 cut(s) 185
BseXI GCAGC 2 cut(s) 304, 409
BshNI GGYRCC 1 cut(s) 94
BsiSI CCGG 1 cut(s) 98
BslI CCNNNNNNNGG 1 cut(s) 188
Bsp143I GATC 1 cut(s) 249
BspACI CCGC 2 cut(s) 92, 342
BspCNI CTCAG 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 96
BspMAI CTGCAG 1 cut(s) 322
BspT107I GGYRCC 1 cut(s) 94
BspTI CTTAAG 1 cut(s) 76
BsrFI RCCGGY 1 cut(s) 97
BssAI RCCGGY 1 cut(s) 97
BssMI GATC 1 cut(s) 249
BssNI GRCGYC 1 cut(s) 95
Bst4CI ACNGT 2 cut(s) 46, 357
BstACI GRCGYC 1 cut(s) 95
BstAFI CTTAAG 1 cut(s) 76
BstC8I GCNNGC 2 cut(s) 99, 315
BstDEI CTNAG 2 cut(s) 171, 246
BstF5I GGATG 1 cut(s) 271
BstH2I RGCGCY 1 cut(s) 98
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 1 cut(s) 252
BstMBI GATC 1 cut(s) 249
BstMWI GCNNNNNNNGC 2 cut(s) 98, 323
BstSFI CTRYAG 1 cut(s) 318
BstV1I GCAGC 2 cut(s) 304, 409
BsuI GTATCC 1 cut(s) 191
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 1 cut(s) 271
Cac8I GCNNGC 2 cut(s) 99, 315
CfoI GCGC 1 cut(s) 97
Cfr10I RCCGGY 1 cut(s) 97
CviAII CATG 2 cut(s) 275, 413
CviJI RGCY 7 cut(s) 117, 209, 215, 284, 317, 326, 422
CviKI_1 RGCY 7 cut(s) 117, 209, 215, 284, 317, 326, 422
DdeI CTNAG 2 cut(s) 171, 246
DinI GGCGCC 1 cut(s) 96
DpnI GATC 1 cut(s) 251
DpnII GATC 1 cut(s) 249
EgeI GGCGCC 1 cut(s) 96
EheI GGCGCC 1 cut(s) 96
FaeI CATG 2 cut(s) 278, 416
FaiI YATR 7 cut(s) 204, 206, 237, 261, 276, 369, 414
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FatI CATG 2 cut(s) 274, 412
FauNDI CATATG 1 cut(s) 204
FblI GTMKAC 1 cut(s) 271
Fnu4HI GCNGC 3 cut(s) 93, 318, 423
FokI GGATG 1 cut(s) 278
Fsp4HI GCNGC 3 cut(s) 93, 318, 423
FspBI CTAG 2 cut(s) 33, 114
GlaI GCGC 1 cut(s) 96
GluI GCNGC 3 cut(s) 93, 318, 423
HaeII RGCGCY 1 cut(s) 98
HapII CCGG 1 cut(s) 98
HhaI GCGC 1 cut(s) 97
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 2 cut(s) 278, 416
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HindIII AAGCTT 1 cut(s) 282
HinfI GANTC 4 cut(s) 60, 122, 192, 228
HpaII CCGG 1 cut(s) 98
Hpy166II GTNNAC 4 cut(s) 131, 272, 298, 349
Hpy188I TCNGA 3 cut(s) 59, 121, 197
Hpy188III TCNNGA 1 cut(s) 33
Hpy8I GTNNAC 4 cut(s) 131, 272, 298, 349
HpyCH4III ACNGT 2 cut(s) 46, 357
HpyCH4V TGCA 1 cut(s) 320
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 323
HpyF3I CTNAG 2 cut(s) 171, 246
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 2 cut(s) 278, 416
HspAI GCGC 1 cut(s) 95
KasI GGCGCC 1 cut(s) 94
KroI GCCGGC 1 cut(s) 97
KroNI GCCGGC 1 cut(s) 99
Kzo9I GATC 1 cut(s) 249
LmnI GCTCC 3 cut(s) 122, 331, 397
LpnPI CCDG 3 cut(s) 111, 392, 408
Lsp1109I GCAGC 2 cut(s) 304, 409
MaeI CTAG 2 cut(s) 33, 114
MalI GATC 1 cut(s) 251
MboI GATC 1 cut(s) 249
MboII GAAGA 4 cut(s) 153, 160, 163, 346
MluCI AATT 3 cut(s) 82, 184, 291
Mly113I GGCGCC 1 cut(s) 95
MlyI GAGTC 1 cut(s) 54
MmeI TCCRAC 1 cut(s) 341
MnlI CCTC 1 cut(s) 415
MreI CGCCGGCG 1 cut(s) 97
MroNI GCCGGC 1 cut(s) 97
MseI TTAA 4 cut(s) 18, 69, 77, 158
MslI CAYNNNNRTG 2 cut(s) 222, 303
MspCI CTTAAG 1 cut(s) 76
MspI CCGG 1 cut(s) 98
MwoI GCNNNNNNNGC 2 cut(s) 98, 323
NaeI GCCGGC 1 cut(s) 99
NarI GGCGCC 1 cut(s) 95
NdeI CATATG 1 cut(s) 204
NdeII GATC 1 cut(s) 249
NgoMIV GCCGGC 1 cut(s) 97
NlaIII CATG 2 cut(s) 278, 416
NlaIV GGNNCC 1 cut(s) 96
PcsI WCGNNNNNNNCGW 1 cut(s) 131
PdiI GCCGGC 1 cut(s) 99
PfeI GAWTC 3 cut(s) 122, 192, 228
PkrI GCNGC 3 cut(s) 94, 319, 424
PleI GAGTC 1 cut(s) 54
PluTI GGCGCC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 54
PspN4I GGNNCC 1 cut(s) 96
PstI CTGCAG 1 cut(s) 322
RseI CAYNNNNRTG 2 cut(s) 222, 303
SaqAI TTAA 4 cut(s) 18, 69, 77, 158
SatI GCNGC 3 cut(s) 93, 318, 423
Sau3AI GATC 1 cut(s) 249
SchI GAGTC 1 cut(s) 54
SetI ASST 9 cut(s) 119, 184, 217, 286, 319, 328, 381, 407, 424
SfcI CTRYAG 1 cut(s) 318
SfoI GGCGCC 1 cut(s) 96
SgrAI CRCCGGYG 1 cut(s) 97
SmiMI CAYNNNNRTG 2 cut(s) 222, 303
SmlI CTYRAG 1 cut(s) 76
SmoI CTYRAG 1 cut(s) 76
Sse9I AATT 3 cut(s) 82, 184, 291
SsiI CCGC 2 cut(s) 92, 342
SspDI GGCGCC 1 cut(s) 94
SspMI CTAG 2 cut(s) 33, 114
TaaI ACNGT 2 cut(s) 46, 357
TaqI TCGA 2 cut(s) 125, 162
TasI AATT 3 cut(s) 82, 184, 291
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 3 cut(s) 122, 192, 228
Tru1I TTAA 4 cut(s) 18, 69, 77, 158
Tru9I TTAA 4 cut(s) 18, 69, 77, 158
TseI GCWGC 2 cut(s) 317, 422
TspDTI ATGAA 1 cut(s) 252
Vha464I CTTAAG 1 cut(s) 76
XapI RAATTY 1 cut(s) 82
XbaI TCTAGA 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 271
XspI CTAG 2 cut(s) 33, 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.