AT5G58990

Mitochondrial 28S ribosomal protein S34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009129
Physical Location & Seq
Forward (+)
23814763 .. 23816190
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58990.1

Sequence Viewer

Length: 447 bp
ATGGCGATGACACTTATGAACAGAGCAATCTCTAGAACCGAAACTGTTGGTGCTTTCAGTCTCTCGCTTAGTCTCTTAAGAAATTTCTCTGCGGCGCCGTCGGCGGCATCTACTCCGGCGAGTGAAAACCCTAGCTCCGATTCGAATAAACCTAAACGAAGAAAGAAGAAAAACTTAATCGAAGTCGCTCAGTTCTTACCTAATTGGGGAATCGGATACCATATGGCTAAAGCTCACTGGAATGGAATCTCTTATGAAATCACTAAGATCAATCTCTATAAGGATGGTAGACATGGAAAAGCTTGGGGAATTGTTCACAAAGATGGATTGCGAGCTGCGGAAGCTCCAAAGAAGATAAGCGGAGTTCACAAACGTTGTTGGAAGTATATCCCAAACCTGTCAAAGACTACTCCTGCAACAACCACTGCTGATGTTCAAGCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S34

Protein Analysis

148

Amino Acids

16.25

Weight (kDa)

10.23

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S34 PF16053 57 - 132 4.1e-08 Mitochondrial 28S ribosomal protein S34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 94
AccI GTMKAC 1 cut(s) 289
AciI CCGC 4 cut(s) 92, 104, 338, 360
AclI AACGTT 1 cut(s) 373
AcsI RAATTY 1 cut(s) 82
AcyI GRCGYC 1 cut(s) 95
AfiI CCNNNNNNNGG 1 cut(s) 206
AflII CTTAAG 1 cut(s) 76
AgsI TTSAA 1 cut(s) 437
AluBI AGCT 6 cut(s) 135, 233, 302, 335, 344, 440
AluI AGCT 6 cut(s) 135, 233, 302, 335, 344, 440
Alw26I GTCTC 2 cut(s) 65, 77
ApeKI GCWGC 2 cut(s) 335, 440
ApoI RAATTY 1 cut(s) 82
AspLEI GCGC 1 cut(s) 97
AsuII TTCGAA 1 cut(s) 143
BanI GGYRCC 1 cut(s) 94
BbvI GCAGC 2 cut(s) 322, 427
BccI CCATC 2 cut(s) 278, 317
BceAI ACGGC 1 cut(s) 82
BciVI GTATCC 1 cut(s) 209
BcoDI GTCTC 2 cut(s) 65, 77
BfaI CTAG 2 cut(s) 33, 132
BfoI RGCGCY 1 cut(s) 98
BfrI CTTAAG 1 cut(s) 76
BfuI GTATCC 1 cut(s) 209
BisI GCNGC 4 cut(s) 93, 105, 336, 441
BlsI GCNGC 4 cut(s) 94, 106, 337, 442
BmiI GGNNCC 1 cut(s) 96
BmsI GCATC 1 cut(s) 116
Bpu14I TTCGAA 1 cut(s) 143
BsaHI GRCGYC 1 cut(s) 95
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 242
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 1 cut(s) 242
BseXI GCAGC 2 cut(s) 322, 427
BshNI GGYRCC 1 cut(s) 94
BsiSI CCGG 1 cut(s) 116
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 2 cut(s) 65, 77
Bsp119I TTCGAA 1 cut(s) 143
Bsp143I GATC 1 cut(s) 267
BspACI CCGC 4 cut(s) 92, 104, 338, 360
BspCNI CTCAG 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 96
BspT104I TTCGAA 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 94
BspTI CTTAAG 1 cut(s) 76
BsrI ACTGG 1 cut(s) 242
BssMI GATC 1 cut(s) 267
BssNI GRCGYC 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 46
BstACI GRCGYC 1 cut(s) 95
BstAFI CTTAAG 1 cut(s) 76
BstBI TTCGAA 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 333
BstDEI CTNAG 3 cut(s) 68, 189, 264
BstF5I GGATG 1 cut(s) 289
BstH2I RGCGCY 1 cut(s) 98
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 1 cut(s) 270
BstMAI GTCTC 2 cut(s) 65, 77
BstMBI GATC 1 cut(s) 267
BstMWI GCNNNNNNNGC 2 cut(s) 101, 341
BstV1I GCAGC 2 cut(s) 322, 427
BsuI GTATCC 1 cut(s) 209
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 1 cut(s) 289
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 2 cut(s) 235, 423
Cac8I GCNNGC 1 cut(s) 333
CfoI GCGC 1 cut(s) 97
CviAII CATG 1 cut(s) 293
CviJI RGCY 7 cut(s) 135, 227, 233, 302, 335, 344, 440
CviKI_1 RGCY 7 cut(s) 135, 227, 233, 302, 335, 344, 440
DdeI CTNAG 3 cut(s) 68, 189, 264
DinI GGCGCC 1 cut(s) 96
DpnI GATC 1 cut(s) 269
DpnII GATC 1 cut(s) 267
EgeI GGCGCC 1 cut(s) 96
EheI GGCGCC 1 cut(s) 96
FaeI CATG 1 cut(s) 296
FaiI YATR 7 cut(s) 17, 222, 224, 255, 279, 294, 387
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FatI CATG 1 cut(s) 292
FauNDI CATATG 1 cut(s) 222
FblI GTMKAC 1 cut(s) 289
Fnu4HI GCNGC 4 cut(s) 93, 105, 336, 441
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 4 cut(s) 93, 105, 336, 441
FspBI CTAG 2 cut(s) 33, 132
GlaI GCGC 1 cut(s) 96
GluI GCNGC 4 cut(s) 93, 105, 336, 441
HaeII RGCGCY 1 cut(s) 98
HapII CCGG 1 cut(s) 116
HhaI GCGC 1 cut(s) 97
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 1 cut(s) 296
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HindIII AAGCTT 1 cut(s) 300
HinfI GANTC 3 cut(s) 140, 210, 246
HpaII CCGG 1 cut(s) 116
Hpy166II GTNNAC 3 cut(s) 290, 316, 367
Hpy188I TCNGA 2 cut(s) 139, 215
Hpy188III TCNNGA 1 cut(s) 33
Hpy8I GTNNAC 3 cut(s) 290, 316, 367
Hpy99I CGWCG 1 cut(s) 103
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 1 cut(s) 416
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 341
HpyF3I CTNAG 3 cut(s) 68, 189, 264
HpySE526I ACGT 1 cut(s) 373
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 1 cut(s) 296
HspAI GCGC 1 cut(s) 95
KasI GGCGCC 1 cut(s) 94
Kzo9I GATC 1 cut(s) 267
LmnI GCTCC 2 cut(s) 140, 349
LpnPI CCDG 4 cut(s) 129, 223, 410, 426
Lsp1109I GCAGC 2 cut(s) 322, 427
LweI GCATC 1 cut(s) 116
MaeI CTAG 2 cut(s) 33, 132
MaeII ACGT 1 cut(s) 373
MalI GATC 1 cut(s) 269
MboI GATC 1 cut(s) 267
MboII GAAGA 3 cut(s) 171, 178, 364
MluCI AATT 3 cut(s) 82, 202, 309
Mly113I GGCGCC 1 cut(s) 95
MmeI TCCRAC 1 cut(s) 359
MseI TTAA 2 cut(s) 77, 176
MslI CAYNNNNRTG 2 cut(s) 240, 321
MspCI CTTAAG 1 cut(s) 76
MspI CCGG 1 cut(s) 116
MwoI GCNNNNNNNGC 2 cut(s) 101, 341
NarI GGCGCC 1 cut(s) 95
NdeI CATATG 1 cut(s) 222
NdeII GATC 1 cut(s) 267
NlaIII CATG 1 cut(s) 296
NlaIV GGNNCC 1 cut(s) 96
NspV TTCGAA 1 cut(s) 143
PfeI GAWTC 3 cut(s) 140, 210, 246
PkrI GCNGC 4 cut(s) 94, 106, 337, 442
PluTI GGCGCC 1 cut(s) 98
Psp1406I AACGTT 1 cut(s) 373
PspN4I GGNNCC 1 cut(s) 96
RseI CAYNNNNRTG 2 cut(s) 240, 321
SaqAI TTAA 2 cut(s) 77, 176
SatI GCNGC 4 cut(s) 93, 105, 336, 441
Sau3AI GATC 1 cut(s) 267
SfaNI GCATC 1 cut(s) 116
SfoI GGCGCC 1 cut(s) 96
SfuI TTCGAA 1 cut(s) 143
SmiMI CAYNNNNRTG 2 cut(s) 240, 321
SmlI CTYRAG 1 cut(s) 76
SmoI CTYRAG 1 cut(s) 76
Sse9I AATT 3 cut(s) 82, 202, 309
SsiI CCGC 4 cut(s) 92, 104, 338, 360
SspDI GGCGCC 1 cut(s) 94
SspMI CTAG 2 cut(s) 33, 132
TaaI ACNGT 1 cut(s) 46
TaiI ACGT 1 cut(s) 376
TaqI TCGA 2 cut(s) 143, 180
TasI AATT 3 cut(s) 82, 202, 309
TauI GCSGC 2 cut(s) 95, 107
TfiI GAWTC 3 cut(s) 140, 210, 246
Tru1I TTAA 2 cut(s) 77, 176
Tru9I TTAA 2 cut(s) 77, 176
TscAI CASTG 2 cut(s) 242, 430
TseI GCWGC 2 cut(s) 335, 440
TspDTI ATGAA 2 cut(s) 32, 270
TspRI CASTG 2 cut(s) 242, 430
Vha464I CTTAAG 1 cut(s) 76
XapI RAATTY 1 cut(s) 82
XbaI TCTAGA 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 289
XspI CTAG 2 cut(s) 33, 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.