MD03G1080100.v1.1

Mitochondrial 28S ribosomal protein S34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009129
Physical Location & Seq
Forward (+)
6451642 .. 6453562
1921 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1080100.v1.1.491

Sequence Viewer

Length: 462 bp
ATGGCGAGCAGTCTCGCAAACAGAGCATTAACAGATCGAACCGGAAGCCTCTGGAGGCTCACATCGAGCTTCCTCCGAAGCTTCAGCACCGCTTCCCAAAACCCACCCGCCGGCGCCGCTTCGCCTTCGGCGTCAAAGAAACCCAAGCGGAAGAAGAAGAAGAACCTGTTCGAGGTGGCCCAGTTTCTGCCCAACTGGGGATTGGGGTACCAAATGGCTAAGACCCACTGGGTCGGCGTCTCTTACCAGATTACAAAAATCAATCTTTACAAGGATGGTAGACATGGCAAAGCATGGGGAATCGTTCATAAGGAAGGCTTGCCAGCCGCAGATGCCCCCAAGAAAATAAGCGGAGTCCACAAGCGTGGTTGGAGGTACATTCCAAACTCGAAGACTGAAATTGTTCCAAGTATGACTAAACGTACGGAAAGTGCTCCAGCAGCTGAAGTTCAAGCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S34

Protein Analysis

154

Amino Acids

16.79

Weight (kDa)

10.59

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S34 PF16053 54 - 142 7.6e-11 Mitochondrial 28S ribosomal protein S34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 207
AccB1I GGYRCC 2 cut(s) 113, 207
AccI GTMKAC 1 cut(s) 280
AciI CCGC 6 cut(s) 90, 108, 117, 148, 327, 351
AcuI CTGAAG 1 cut(s) 67
AcyI GRCGYC 3 cut(s) 114, 131, 237
AfaI GTAC 3 cut(s) 209, 377, 424
AfiI CCNNNNNNNGG 3 cut(s) 110, 172, 197
AgsI TTSAA 1 cut(s) 452
AleI CACNNNNGTG 1 cut(s) 363
AluBI AGCT 4 cut(s) 69, 81, 443, 458
AluI AGCT 4 cut(s) 69, 81, 443, 458
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 2 cut(s) 17, 244
AlwNI CAGNNNCTG 2 cut(s) 187, 443
AoxI GGCC 1 cut(s) 177
ApeKI GCWGC 2 cut(s) 440, 455
Asp700I GAANNNNTTC 2 cut(s) 167, 402
Asp718I GGTACC 1 cut(s) 207
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 178
BanI GGYRCC 2 cut(s) 113, 207
BbsI GAAGAC 1 cut(s) 398
Bbv12I GWGCWC 1 cut(s) 436
BbvI GCAGC 1 cut(s) 452
BccI CCATC 1 cut(s) 269
BcoDI GTCTC 2 cut(s) 17, 244
BfoI RGCGCY 1 cut(s) 117
BisI GCNGC 4 cut(s) 117, 327, 441, 456
BlsI GCNGC 4 cut(s) 118, 328, 442, 457
BmgT120I GGNCC 1 cut(s) 178
BmiI GGNNCC 2 cut(s) 115, 209
BmrI ACTGGG 3 cut(s) 175, 205, 238
BmsI GCATC 1 cut(s) 322
BmuI ACTGGG 3 cut(s) 175, 205, 238
BpiI GAAGAC 1 cut(s) 398
BpmI CTGGAG 2 cut(s) 73, 420
BsaHI GRCGYC 3 cut(s) 114, 131, 237
BsaWI WCCGGW 1 cut(s) 41
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 172, 197
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 3 cut(s) 181, 200, 233
BseGI GGATG 1 cut(s) 280
BseLI CCNNNNNNNGG 3 cut(s) 110, 172, 197
BseNI ACTGG 3 cut(s) 181, 200, 233
BseXI GCAGC 1 cut(s) 452
BshFI GGCC 1 cut(s) 179
BshNI GGYRCC 2 cut(s) 113, 207
BsiHKAI GWGCWC 1 cut(s) 436
BsiSI CCGG 2 cut(s) 42, 111
BsiWI CGTACG 1 cut(s) 422
BslI CCNNNNNNNGG 3 cut(s) 110, 172, 197
BsmAI GTCTC 2 cut(s) 17, 244
BsmBI CGTCTC 1 cut(s) 244
BsnI GGCC 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 1 cut(s) 34
BspACI CCGC 6 cut(s) 90, 108, 117, 148, 327, 351
BspANI GGCC 1 cut(s) 179
BspLI GGNNCC 2 cut(s) 115, 209
BspT107I GGYRCC 2 cut(s) 113, 207
BsrFI RCCGGY 1 cut(s) 110
BsrI ACTGG 3 cut(s) 181, 200, 233
BssAI RCCGGY 1 cut(s) 110
BssMI GATC 1 cut(s) 34
BssNI GRCGYC 3 cut(s) 114, 131, 237
BstACI GRCGYC 3 cut(s) 114, 131, 237
BstC8I GCNNGC 4 cut(s) 7, 112, 320, 324
BstDEI CTNAG 1 cut(s) 219
BstENI CCTNNNNNAGG 1 cut(s) 170
BstF5I GGATG 1 cut(s) 280
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 1 cut(s) 37
BstMAI GTCTC 2 cut(s) 17, 244
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 4 cut(s) 23, 116, 332, 440
BstV1I GCAGC 1 cut(s) 452
BstV2I GAAGAC 1 cut(s) 398
BstXI CCANNNNNNTGG 1 cut(s) 365
BsuRI GGCC 1 cut(s) 179
BtsCI GGATG 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 226
Cac8I GCNNGC 4 cut(s) 7, 112, 320, 324
CaiI CAGNNNCTG 2 cut(s) 187, 443
CfoI GCGC 1 cut(s) 116
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 1 cut(s) 178
CseI GACGC 2 cut(s) 120, 226
Csp6I GTAC 3 cut(s) 208, 376, 423
CviAII CATG 2 cut(s) 284, 294
CviQI GTAC 3 cut(s) 208, 376, 423
DdeI CTNAG 1 cut(s) 219
DinI GGCGCC 1 cut(s) 115
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 170
EgeI GGCGCC 1 cut(s) 115
EheI GGCGCC 1 cut(s) 115
Esp3I CGTCTC 1 cut(s) 244
FaeI CATG 2 cut(s) 287, 297
FaiI YATR 4 cut(s) 285, 295, 309, 413
FalI AAGNNNNNCTT 2 cut(s) 302, 334
FatI CATG 2 cut(s) 283, 293
FauI CCCGC 1 cut(s) 115
FblI GTMKAC 1 cut(s) 280
Fnu4HI GCNGC 4 cut(s) 117, 327, 441, 456
FokI GGATG 1 cut(s) 287
Fsp4HI GCNGC 4 cut(s) 117, 327, 441, 456
GlaI GCGC 1 cut(s) 115
GluI GCNGC 4 cut(s) 117, 327, 441, 456
GsuI CTGGAG 2 cut(s) 73, 420
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 1 cut(s) 179
HapII CCGG 2 cut(s) 42, 111
HgaI GACGC 2 cut(s) 120, 226
HhaI GCGC 1 cut(s) 116
Hin1I GRCGYC 3 cut(s) 114, 131, 237
Hin1II CATG 2 cut(s) 287, 297
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 2 cut(s) 300, 354
HpaII CCGG 2 cut(s) 42, 111
Hpy166II GTNNAC 2 cut(s) 281, 358
Hpy188I TCNGA 1 cut(s) 77
Hpy188III TCNNGA 1 cut(s) 52
Hpy8I GTNNAC 2 cut(s) 281, 358
HpyAV CCTTC 2 cut(s) 135, 308
HpyCH4IV ACGT 1 cut(s) 421
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 116, 332, 440
HpyF3I CTNAG 1 cut(s) 219
HpySE526I ACGT 1 cut(s) 421
Hsp92I GRCGYC 3 cut(s) 114, 131, 237
Hsp92II CATG 2 cut(s) 287, 297
HspAI GCGC 1 cut(s) 114
KasI GGCGCC 1 cut(s) 113
KpnI GGTACC 1 cut(s) 211
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
Kzo9I GATC 1 cut(s) 34
LmnI GCTCC 1 cut(s) 439
Lsp1109I GCAGC 1 cut(s) 452
LweI GCATC 1 cut(s) 322
MaeII ACGT 1 cut(s) 421
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MboII GAAGA 5 cut(s) 163, 166, 169, 172, 403
MhlI GDGCHC 1 cut(s) 436
MluCI AATT 1 cut(s) 399
Mly113I GGCGCC 1 cut(s) 114
MlyI GAGTC 1 cut(s) 363
MmeI TCCRAC 1 cut(s) 350
MnlI CCTC 5 cut(s) 48, 59, 83, 166, 366
MreI CGCCGGCG 1 cut(s) 110
MroNI GCCGGC 1 cut(s) 110
MroXI GAANNNNTTC 2 cut(s) 167, 402
MseI TTAA 1 cut(s) 29
MslI CAYNNNNRTG 1 cut(s) 363
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 2 cut(s) 42, 111
MwoI GCNNNNNNNGC 4 cut(s) 23, 116, 332, 440
NaeI GCCGGC 1 cut(s) 112
NarI GGCGCC 1 cut(s) 114
NdeII GATC 1 cut(s) 34
NgoMIV GCCGGC 1 cut(s) 110
NlaIII CATG 2 cut(s) 287, 297
NlaIV GGNNCC 2 cut(s) 115, 209
OliI CACNNNNGTG 1 cut(s) 363
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PdiI GCCGGC 1 cut(s) 112
PdmI GAANNNNTTC 2 cut(s) 167, 402
PfeI GAWTC 1 cut(s) 300
Pfl23II CGTACG 1 cut(s) 422
PkrI GCNGC 4 cut(s) 118, 328, 442, 457
PleI GAGTC 1 cut(s) 362
PluTI GGCGCC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 362
PspLI CGTACG 1 cut(s) 422
PspN4I GGNNCC 2 cut(s) 115, 209
PspPI GGNCC 1 cut(s) 178
PstNI CAGNNNCTG 2 cut(s) 187, 443
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 3 cut(s) 209, 377, 424
RsaNI GTAC 3 cut(s) 208, 376, 423
RseI CAYNNNNRTG 1 cut(s) 363
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 4 cut(s) 117, 327, 441, 456
Sau3AI GATC 1 cut(s) 34
Sau96I GGNCC 1 cut(s) 178
SchI GAGTC 1 cut(s) 363
SduI GDGCHC 1 cut(s) 436
SetI ASST 8 cut(s) 71, 83, 168, 177, 377, 424, 445, 460
SfaNI GCATC 1 cut(s) 322
SfoI GGCGCC 1 cut(s) 115
SgrAI CRCCGGYG 1 cut(s) 110
SmiMI CAYNNNNRTG 1 cut(s) 363
Sse9I AATT 1 cut(s) 399
SsiI CCGC 6 cut(s) 90, 108, 117, 148, 327, 351
SspDI GGCGCC 1 cut(s) 113
TaiI ACGT 1 cut(s) 424
TaqI TCGA 4 cut(s) 37, 65, 171, 389
TasI AATT 1 cut(s) 399
TauI GCSGC 2 cut(s) 119, 329
TfiI GAWTC 1 cut(s) 300
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TscAI CASTG 1 cut(s) 233
TseI GCWGC 2 cut(s) 440, 455
TspDTI ATGAA 1 cut(s) 296
TspGWI ACGGA 1 cut(s) 440
TspRI CASTG 1 cut(s) 233
XagI CCTNNNNNAGG 1 cut(s) 170
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 280
XmnI GAANNNNTTC 2 cut(s) 167, 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.