FvH4_3g37450

Mitochondrial 28S ribosomal protein S34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009129
Physical Location & Seq
Reverse (-)
32134044 .. 32136018
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g37450.t1

Sequence Viewer

Length: 471 bp
ATGGCGAGCAGTCTCTCACACAGAGCGATAGCCGATCGAGGCTTATTGAGGCTCACGTTGAGCTTCGTCAGAACCTTCAGCTCCGCTTCCCCAAACCCTACCGACGCCGCCGCCGGCGCCTCTTCGCCGGCGGCTACTAAACCGAAGAAGAGAAAGAAGAAGAACCTGTTCGAGGTGGCACAGTATCTGCCCAATTGGGGACTTGGGTACCACATGGCTAAGAGCCACTGGGTCGGCGTGTCGTATGAGATCACCAAGATCAATCTTTACAAGGATGGAAGGCATGGAAAGGCATGGGGAGTTGTTCATAAGGAAGGCTTGCCAGCTGCAGACACCCCCAAGAAAATAAGTGGAGTCCACAAGCGTTGTTGGAGGTACATTCCAAGCTTATCTAAATCAACTGAAAGTGTTCCAAGTATGACTAAACCAACAGAAACTGCTCAAGCAACACCTGAAGTTCAACCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S34

Protein Analysis

157

Amino Acids

17.03

Weight (kDa)

10.12

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S34 PF16053 54 - 131 2.5e-09 Mitochondrial 28S ribosomal protein S34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 207
AccB1I GGYRCC 2 cut(s) 116, 207
AciI CCGC 4 cut(s) 84, 108, 111, 131
AcuI CTGAAG 1 cut(s) 61
AcyI GRCGYC 2 cut(s) 105, 117
AfaI GTAC 2 cut(s) 209, 377
AfiI CCNNNNNNNGG 2 cut(s) 172, 197
AgsI TTSAA 1 cut(s) 461
AluBI AGCT 5 cut(s) 63, 81, 326, 387, 467
AluI AGCT 5 cut(s) 63, 81, 326, 387, 467
Alw26I GTCTC 1 cut(s) 17
AlwNI CAGNNNCTG 2 cut(s) 187, 437
ApeKI GCWGC 1 cut(s) 326
Asp700I GAANNNNTTC 2 cut(s) 167, 408
Asp718I GGTACC 1 cut(s) 207
AspLEI GCGC 1 cut(s) 119
AsuHPI GGTGA 1 cut(s) 244
BaeI ACNNNNGTAYC 2 cut(s) 191, 224
BanI GGYRCC 2 cut(s) 116, 207
BbvI GCAGC 1 cut(s) 313
BccI CCATC 1 cut(s) 269
BcoDI GTCTC 1 cut(s) 17
BfmI CTRYAG 1 cut(s) 327
BfoI RGCGCY 1 cut(s) 120
BisI GCNGC 4 cut(s) 108, 111, 132, 327
BlsI GCNGC 4 cut(s) 109, 112, 133, 328
BmiI GGNNCC 2 cut(s) 118, 209
BmrI ACTGGG 1 cut(s) 238
BmuI ACTGGG 1 cut(s) 238
BpuEI CTTGAG 1 cut(s) 426
BsaHI GRCGYC 2 cut(s) 105, 117
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 197
Bse118I RCCGGY 2 cut(s) 113, 127
Bse1I ACTGG 1 cut(s) 233
BseGI GGATG 1 cut(s) 280
BseLI CCNNNNNNNGG 2 cut(s) 172, 197
BseNI ACTGG 1 cut(s) 233
BseXI GCAGC 1 cut(s) 313
Bsh1285I CGRYCG 1 cut(s) 37
BshNI GGYRCC 2 cut(s) 116, 207
BsiEI CGRYCG 1 cut(s) 37
BsiSI CCGG 2 cut(s) 114, 128
BslFI GGGAC 1 cut(s) 213
BslI CCNNNNNNNGG 2 cut(s) 172, 197
BsmAI GTCTC 1 cut(s) 17
BsmFI GGGAC 1 cut(s) 213
Bsp143I GATC 3 cut(s) 34, 249, 258
BspACI CCGC 4 cut(s) 84, 108, 111, 131
BspLI GGNNCC 2 cut(s) 118, 209
BspMAI CTGCAG 1 cut(s) 331
BspT107I GGYRCC 2 cut(s) 116, 207
BsrFI RCCGGY 2 cut(s) 113, 127
BsrI ACTGG 1 cut(s) 233
BssAI RCCGGY 2 cut(s) 113, 127
BssMI GATC 3 cut(s) 34, 249, 258
BssNI GRCGYC 2 cut(s) 105, 117
Bst4CI ACNGT 1 cut(s) 183
Bst6I CTCTTC 2 cut(s) 127, 143
BstACI GRCGYC 2 cut(s) 105, 117
BstC8I GCNNGC 5 cut(s) 7, 115, 129, 320, 324
BstDEI CTNAG 1 cut(s) 219
BstENI CCTNNNNNAGG 1 cut(s) 170
BstF5I GGATG 1 cut(s) 280
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 3 cut(s) 37, 252, 261
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 3 cut(s) 34, 249, 258
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstSFI CTRYAG 1 cut(s) 327
BstV1I GCAGC 1 cut(s) 313
BtsCI GGATG 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 226
Cac8I GCNNGC 5 cut(s) 7, 115, 129, 320, 324
CaiI CAGNNNCTG 2 cut(s) 187, 437
CfoI GCGC 1 cut(s) 119
Cfr10I RCCGGY 2 cut(s) 113, 127
CseI GACGC 1 cut(s) 113
Csp6I GTAC 2 cut(s) 208, 376
CspCI CAANNNNNGTGG 2 cut(s) 347, 382
CviAII CATG 3 cut(s) 214, 284, 294
CviQI GTAC 2 cut(s) 208, 376
DdeI CTNAG 1 cut(s) 219
DinI GGCGCC 1 cut(s) 118
DpnI GATC 3 cut(s) 36, 251, 260
DpnII GATC 3 cut(s) 34, 249, 258
Eam1104I CTCTTC 2 cut(s) 127, 143
EarI CTCTTC 2 cut(s) 127, 143
Eco57I CTGAAG 1 cut(s) 61
EcoNI CCTNNNNNAGG 1 cut(s) 170
EgeI GGCGCC 1 cut(s) 118
EheI GGCGCC 1 cut(s) 118
FaeI CATG 3 cut(s) 217, 287, 297
FaiI YATR 6 cut(s) 215, 246, 285, 295, 309, 419
FalI AAGNNNNNCTT 2 cut(s) 302, 334
FaqI GGGAC 1 cut(s) 213
FatI CATG 3 cut(s) 213, 283, 293
Fnu4HI GCNGC 4 cut(s) 108, 111, 132, 327
FokI GGATG 1 cut(s) 287
Fsp4HI GCNGC 4 cut(s) 108, 111, 132, 327
GlaI GCGC 1 cut(s) 118
GluI GCNGC 4 cut(s) 108, 111, 132, 327
HaeII RGCGCY 1 cut(s) 120
HapII CCGG 2 cut(s) 114, 128
HgaI GACGC 1 cut(s) 113
HhaI GCGC 1 cut(s) 119
Hin1I GRCGYC 2 cut(s) 105, 117
Hin1II CATG 3 cut(s) 217, 287, 297
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HindIII AAGCTT 1 cut(s) 385
HinfI GANTC 1 cut(s) 354
HpaII CCGG 2 cut(s) 114, 128
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 1 cut(s) 71
Hpy8I GTNNAC 1 cut(s) 358
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 3 cut(s) 85, 273, 308
HpyCH4III ACNGT 1 cut(s) 183
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 1 cut(s) 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 1 cut(s) 219
HpySE526I ACGT 1 cut(s) 56
Hsp92I GRCGYC 2 cut(s) 105, 117
Hsp92II CATG 3 cut(s) 217, 287, 297
HspAI GCGC 1 cut(s) 117
KasI GGCGCC 1 cut(s) 116
KpnI GGTACC 1 cut(s) 211
KroI GCCGGC 2 cut(s) 113, 127
KroNI GCCGGC 2 cut(s) 115, 129
Kzo9I GATC 3 cut(s) 34, 249, 258
LmnI GCTCC 1 cut(s) 86
LpnPI CCDG 6 cut(s) 127, 141, 179, 214, 336, 465
Lsp1109I GCAGC 1 cut(s) 313
MaeII ACGT 1 cut(s) 56
MalI GATC 3 cut(s) 36, 251, 260
MboI GATC 3 cut(s) 34, 249, 258
MboII GAAGA 5 cut(s) 114, 157, 160, 169, 172
MfeI CAATTG 1 cut(s) 193
MluCI AATT 1 cut(s) 193
Mly113I GGCGCC 1 cut(s) 117
MlyI GAGTC 1 cut(s) 363
MmeI TCCRAC 1 cut(s) 350
MnlI CCTC 5 cut(s) 32, 42, 130, 166, 366
MreI CGCCGGCG 2 cut(s) 113, 127
MroNI GCCGGC 2 cut(s) 113, 127
MroXI GAANNNNTTC 2 cut(s) 167, 408
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 2 cut(s) 114, 128
MunI CAATTG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 116
NaeI GCCGGC 2 cut(s) 115, 129
NarI GGCGCC 1 cut(s) 117
NdeII GATC 3 cut(s) 34, 249, 258
NgoMIV GCCGGC 2 cut(s) 113, 127
NlaIII CATG 3 cut(s) 217, 287, 297
NlaIV GGNNCC 2 cut(s) 118, 209
PdiI GCCGGC 2 cut(s) 115, 129
PdmI GAANNNNTTC 2 cut(s) 167, 408
PkrI GCNGC 4 cut(s) 109, 112, 133, 328
Ple19I CGATCG 1 cut(s) 37
PleI GAGTC 1 cut(s) 362
PluTI GGCGCC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 362
PspN4I GGNNCC 2 cut(s) 118, 209
PstI CTGCAG 1 cut(s) 331
PstNI CAGNNNCTG 2 cut(s) 187, 437
PvuI CGATCG 1 cut(s) 37
PvuII CAGCTG 1 cut(s) 326
RsaI GTAC 2 cut(s) 209, 377
RsaNI GTAC 2 cut(s) 208, 376
SatI GCNGC 4 cut(s) 108, 111, 132, 327
Sau3AI GATC 3 cut(s) 34, 249, 258
SchI GAGTC 1 cut(s) 363
SfcI CTRYAG 1 cut(s) 327
SfoI GGCGCC 1 cut(s) 118
SgrAI CRCCGGYG 2 cut(s) 113, 127
SmlI CTYRAG 1 cut(s) 441
SmoI CTYRAG 1 cut(s) 441
Sse9I AATT 1 cut(s) 193
SsiI CCGC 4 cut(s) 84, 108, 111, 131
SspDI GGCGCC 1 cut(s) 116
TaaI ACNGT 1 cut(s) 183
TaiI ACGT 1 cut(s) 59
TaqI TCGA 2 cut(s) 37, 171
TasI AATT 1 cut(s) 193
TauI GCSGC 3 cut(s) 110, 113, 134
TscAI CASTG 1 cut(s) 233
TseI GCWGC 1 cut(s) 326
TspDTI ATGAA 1 cut(s) 296
TspRI CASTG 1 cut(s) 233
XagI CCTNNNNNAGG 1 cut(s) 170
XmnI GAANNNNTTC 2 cut(s) 167, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.