RchiOBHm_Chr5g0067341

Mitochondrial 28S ribosomal protein S34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0009129
Physical Location & Seq
Reverse (-)
73526829 .. 73528560
1732 bp
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UTR
Exon/CDS
Intron
PRQ34306

Sequence Viewer

Length: 480 bp
ATGGCGAGCAGTCTCGCACAGAGGGCATTGACGGATCGAACAGTAGGGTTATTGAGGTTCACGTCGAGCTTCGTCAGAAGCTTCAGCACCGCTTCGCCAAACCCTACTGACGCCGCCGCCGCCGGTTCTTCTCCTCCGGTGGCCACTAAGCCGAAGAAGAGAAAGAAGAAGAACCTGTTCGAGGTGGCACAGTATCTGCCCAATTGGGGACTTGGGTACCACATGGCTAAGAGCCACTGGGTCGGCGTCTCGTATCAGATCACCAAAATCAATCTTTACAAGGATGGTAAGCATGGAAAGGCATGGGGAGTCGTTCATAAGGAAGGCTTGCCAGCTGCAGATGCTCCCAAGAAAATAAGTGGAGTCCACAAGCGTGGTTGGAGGTACATTCCAAGCTTATCTAAGTCGACTGAAAGTGTTCCAAGTATGACTAAACCAACAGAAACTGCTCCAGCAACACCTGAAGTTCAACCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S34

Protein Analysis

159

Amino Acids

17.23

Weight (kDa)

10.23

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S34 PF16053 57 - 134 2e-09 Mitochondrial 28S ribosomal protein S34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 216
AccB1I GGYRCC 1 cut(s) 216
AccI GTMKAC 1 cut(s) 407
AciI CCGC 4 cut(s) 90, 114, 117, 120
AclWI GGATC 1 cut(s) 42
AcoI YGGCCR 1 cut(s) 141
AcuI CTGAAG 1 cut(s) 67
AcyI GRCGYC 2 cut(s) 111, 246
AfaI GTAC 2 cut(s) 218, 386
AfiI CCNNNNNNNGG 2 cut(s) 181, 206
AgsI TTSAA 1 cut(s) 470
AjiI CACGTC 1 cut(s) 63
AleI CACNNNNGTG 1 cut(s) 372
AluBI AGCT 5 cut(s) 69, 81, 335, 396, 476
AluI AGCT 5 cut(s) 69, 81, 335, 396, 476
Alw26I GTCTC 2 cut(s) 17, 253
AlwI GGATC 1 cut(s) 42
AlwNI CAGNNNCTG 2 cut(s) 196, 446
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 335
Asp700I GAANNNNTTC 2 cut(s) 176, 417
Asp718I GGTACC 1 cut(s) 216
AsuHPI GGTGA 1 cut(s) 253
BaeI ACNNNNGTAYC 2 cut(s) 200, 233
BalI TGGCCA 1 cut(s) 143
BanI GGYRCC 1 cut(s) 216
BbvI GCAGC 1 cut(s) 322
BccI CCATC 1 cut(s) 278
BcoDI GTCTC 2 cut(s) 17, 253
BfmI CTRYAG 1 cut(s) 336
BisI GCNGC 4 cut(s) 114, 117, 120, 336
BlsI GCNGC 4 cut(s) 115, 118, 121, 337
BmgBI CACGTC 1 cut(s) 63
BmiI GGNNCC 1 cut(s) 218
BmrI ACTGGG 1 cut(s) 247
BmsI GCATC 1 cut(s) 331
BmuI ACTGGG 1 cut(s) 247
BpmI CTGGAG 1 cut(s) 435
BsaHI GRCGYC 2 cut(s) 111, 246
BsaWI WCCGGW 1 cut(s) 136
Bsc4I CCNNNNNNNGG 2 cut(s) 181, 206
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 242
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 2 cut(s) 181, 206
BseNI ACTGG 1 cut(s) 242
BseRI GAGGAG 1 cut(s) 123
BseXI GCAGC 1 cut(s) 322
BshFI GGCC 1 cut(s) 143
BshNI GGYRCC 1 cut(s) 216
BsiSI CCGG 2 cut(s) 123, 137
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 2 cut(s) 181, 206
BsmAI GTCTC 2 cut(s) 17, 253
BsmBI CGTCTC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 222
BsnI GGCC 1 cut(s) 143
Bsp143I GATC 2 cut(s) 34, 258
BspACI CCGC 4 cut(s) 90, 114, 117, 120
BspANI GGCC 1 cut(s) 143
BspLI GGNNCC 1 cut(s) 218
BspMAI CTGCAG 1 cut(s) 340
BspPI GGATC 1 cut(s) 42
BspT107I GGYRCC 1 cut(s) 216
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 1 cut(s) 242
BssAI RCCGGY 1 cut(s) 122
BssMI GATC 2 cut(s) 34, 258
BssNI GRCGYC 2 cut(s) 111, 246
Bst4CI ACNGT 2 cut(s) 43, 192
Bst6I CTCTTC 1 cut(s) 152
BstACI GRCGYC 2 cut(s) 111, 246
BstC8I GCNNGC 3 cut(s) 7, 329, 333
BstDEI CTNAG 3 cut(s) 147, 228, 402
BstENI CCTNNNNNAGG 1 cut(s) 179
BstF5I GGATG 1 cut(s) 289
BstKTI GATC 2 cut(s) 37, 261
BstMAI GTCTC 2 cut(s) 17, 253
BstMBI GATC 2 cut(s) 34, 258
BstMWI GCNNNNNNNGC 3 cut(s) 23, 119, 341
BstSFI CTRYAG 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 322
BstXI CCANNNNNNTGG 1 cut(s) 374
BsuRI GGCC 1 cut(s) 143
BtrI CACGTC 1 cut(s) 63
BtsCI GGATG 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 235
Cac8I GCNNGC 3 cut(s) 7, 329, 333
CaiI CAGNNNCTG 2 cut(s) 196, 446
Cfr10I RCCGGY 1 cut(s) 122
CseI GACGC 2 cut(s) 119, 235
Csp6I GTAC 2 cut(s) 217, 385
CviAII CATG 3 cut(s) 223, 293, 303
CviQI GTAC 2 cut(s) 217, 385
DdeI CTNAG 3 cut(s) 147, 228, 402
DpnI GATC 2 cut(s) 36, 260
DpnII GATC 2 cut(s) 34, 258
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 179
Esp3I CGTCTC 1 cut(s) 253
FaeI CATG 3 cut(s) 226, 296, 306
FaiI YATR 5 cut(s) 224, 294, 304, 318, 428
FalI AAGNNNNNCTT 2 cut(s) 311, 343
FaqI GGGAC 1 cut(s) 222
FatI CATG 3 cut(s) 222, 292, 302
FblI GTMKAC 1 cut(s) 407
Fnu4HI GCNGC 4 cut(s) 114, 117, 120, 336
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 4 cut(s) 114, 117, 120, 336
GluI GCNGC 4 cut(s) 114, 117, 120, 336
GsuI CTGGAG 1 cut(s) 435
HaeIII GGCC 1 cut(s) 143
HapII CCGG 2 cut(s) 123, 137
HgaI GACGC 2 cut(s) 119, 235
Hin1I GRCGYC 2 cut(s) 111, 246
Hin1II CATG 3 cut(s) 226, 296, 306
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HindIII AAGCTT 2 cut(s) 79, 394
HinfI GANTC 2 cut(s) 309, 363
HpaII CCGG 2 cut(s) 123, 137
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 3 cut(s) 60, 367, 408
Hpy188I TCNGA 2 cut(s) 77, 258
Hpy8I GTNNAC 3 cut(s) 60, 367, 408
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 1 cut(s) 317
HpyCH4III ACNGT 2 cut(s) 43, 192
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 1 cut(s) 338
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 119, 341
HpyF3I CTNAG 3 cut(s) 147, 228, 402
HpySE526I ACGT 1 cut(s) 62
Hsp92I GRCGYC 2 cut(s) 111, 246
Hsp92II CATG 3 cut(s) 226, 296, 306
KpnI GGTACC 1 cut(s) 220
Kzo9I GATC 2 cut(s) 34, 258
LmnI GCTCC 2 cut(s) 349, 454
LpnPI CCDG 7 cut(s) 136, 150, 188, 223, 345, 465, 474
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 1 cut(s) 331
MaeII ACGT 1 cut(s) 62
MalI GATC 2 cut(s) 36, 260
MboI GATC 2 cut(s) 34, 258
MboII GAAGA 5 cut(s) 120, 166, 169, 178, 181
MfeI CAATTG 1 cut(s) 202
MlsI TGGCCA 1 cut(s) 143
MluCI AATT 1 cut(s) 202
MluNI TGGCCA 1 cut(s) 143
MlyI GAGTC 2 cut(s) 318, 372
MmeI TCCRAC 1 cut(s) 359
MnlI CCTC 5 cut(s) 15, 48, 144, 175, 375
Mox20I TGGCCA 1 cut(s) 143
MroXI GAANNNNTTC 2 cut(s) 176, 417
MscI TGGCCA 1 cut(s) 143
MslI CAYNNNNRTG 1 cut(s) 372
Msp20I TGGCCA 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 2 cut(s) 123, 137
MunI CAATTG 1 cut(s) 202
MwoI GCNNNNNNNGC 3 cut(s) 23, 119, 341
NdeII GATC 2 cut(s) 34, 258
NlaIII CATG 3 cut(s) 226, 296, 306
NlaIV GGNNCC 1 cut(s) 218
OliI CACNNNNGTG 1 cut(s) 372
PdmI GAANNNNTTC 2 cut(s) 176, 417
PkrI GCNGC 4 cut(s) 115, 118, 121, 337
PleI GAGTC 2 cut(s) 317, 371
PpsI GAGTC 2 cut(s) 317, 371
PspN4I GGNNCC 1 cut(s) 218
PstI CTGCAG 1 cut(s) 340
PstNI CAGNNNCTG 2 cut(s) 196, 446
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 2 cut(s) 218, 386
RsaNI GTAC 2 cut(s) 217, 385
RseI CAYNNNNRTG 1 cut(s) 372
SalI GTCGAC 1 cut(s) 406
SatI GCNGC 4 cut(s) 114, 117, 120, 336
Sau3AI GATC 2 cut(s) 34, 258
SchI GAGTC 2 cut(s) 318, 372
SfaNI GCATC 1 cut(s) 331
SfcI CTRYAG 1 cut(s) 336
SmiMI CAYNNNNRTG 1 cut(s) 372
Sse9I AATT 1 cut(s) 202
SsiI CCGC 4 cut(s) 90, 114, 117, 120
TaaI ACNGT 2 cut(s) 43, 192
TaiI ACGT 1 cut(s) 65
TaqI TCGA 4 cut(s) 37, 65, 180, 407
TasI AATT 1 cut(s) 202
TauI GCSGC 3 cut(s) 116, 119, 122
TscAI CASTG 1 cut(s) 242
TseI GCWGC 1 cut(s) 335
TspDTI ATGAA 1 cut(s) 305
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 1 cut(s) 242
XagI CCTNNNNNAGG 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 407
XmnI GAANNNNTTC 2 cut(s) 176, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.