Rh5BG460800

Mitochondrial 28S ribosomal protein S34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0009129
Physical Location & Seq
Reverse (-)
73650029 .. 73651719
1691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG460800.1

Sequence Viewer

Length: 483 bp
ATGGCGAGCAGTCTCGCACAGAGAGCAGTGACGGATCGAACAGTAGGGTTATTGAGGCTCACGTCGAGCTTCGTCAGAAGCTTCAGCACCGCTTCGCCAAACCCTACTGACGCCGCGGCCGCCGCCGGTTCTTCTCCTCCGGTGGCCACTAAGCCGAAGAAGAGAAAGAAGAAGAACCTGTTCGAGGTGGCACAGTATCTACCCAATTGGGGACTTGGGTACCACATGGCTAAAAGCCACTGGGTCGGCGTCTCGTATCAGATCACCAAAATCAATCTTTACAAGGATGGTAAGCATGGAAAGGCATGGGGAGTCGTTCATAAGGAAGGCTTGCCAGCTGCAGATGCTCCCAAGAAAATAAGTGGAGTCCACAAGCGTGGTTGGAGGTACATTCCAAGCTTATCTAAATCGACTGAAAGTGTTCCAAGTATGACTAAACCAACAGAAACTGCTCCAGCAACACCTGAAGTTCAACCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP-S34

Protein Analysis

160

Amino Acids

17.25

Weight (kDa)

10.23

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP-S34 PF16053 58 - 135 2e-09 Mitochondrial 28S ribosomal protein S34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 219
AccB1I GGYRCC 1 cut(s) 219
AccII CGCG 1 cut(s) 116
AciI CCGC 5 cut(s) 90, 114, 116, 120, 123
AclWI GGATC 1 cut(s) 42
AcoI YGGCCR 2 cut(s) 117, 144
AcuI CTGAAG 1 cut(s) 67
AcyI GRCGYC 2 cut(s) 111, 249
AfaI GTAC 2 cut(s) 221, 389
AfiI CCNNNNNNNGG 2 cut(s) 184, 209
AgsI TTSAA 1 cut(s) 473
AjiI CACGTC 1 cut(s) 63
AleI CACNNNNGTG 1 cut(s) 375
AluBI AGCT 5 cut(s) 69, 81, 338, 399, 479
AluI AGCT 5 cut(s) 69, 81, 338, 399, 479
Alw26I GTCTC 2 cut(s) 17, 256
AlwI GGATC 1 cut(s) 42
AlwNI CAGNNNCTG 1 cut(s) 449
AoxI GGCC 2 cut(s) 117, 144
ApeKI GCWGC 1 cut(s) 338
Asp700I GAANNNNTTC 2 cut(s) 179, 420
Asp718I GGTACC 1 cut(s) 219
AsuHPI GGTGA 1 cut(s) 256
BaeI ACNNNNGTAYC 2 cut(s) 203, 236
BalI TGGCCA 1 cut(s) 146
BanI GGYRCC 1 cut(s) 219
BbvI GCAGC 1 cut(s) 325
BccI CCATC 1 cut(s) 281
BcoDI GTCTC 2 cut(s) 17, 256
BfmI CTRYAG 1 cut(s) 339
BisI GCNGC 5 cut(s) 114, 117, 120, 123, 339
BlsI GCNGC 5 cut(s) 115, 118, 121, 124, 340
BmgBI CACGTC 1 cut(s) 63
BmiI GGNNCC 1 cut(s) 221
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 1 cut(s) 334
BmuI ACTGGG 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 438
BsaHI GRCGYC 2 cut(s) 111, 249
BsaJI CCNNGG 1 cut(s) 114
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 209
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 245
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 2 cut(s) 184, 209
BseNI ACTGG 1 cut(s) 245
BseRI GAGGAG 1 cut(s) 126
BseX3I CGGCCG 1 cut(s) 117
BseXI GCAGC 1 cut(s) 325
Bsh1236I CGCG 1 cut(s) 116
Bsh1285I CGRYCG 1 cut(s) 120
BshFI GGCC 2 cut(s) 119, 146
BshNI GGYRCC 1 cut(s) 219
BsiEI CGRYCG 1 cut(s) 120
BsiSI CCGG 2 cut(s) 126, 140
BslFI GGGAC 1 cut(s) 225
BslI CCNNNNNNNGG 2 cut(s) 184, 209
BsmAI GTCTC 2 cut(s) 17, 256
BsmBI CGTCTC 1 cut(s) 256
BsmFI GGGAC 1 cut(s) 225
BsnI GGCC 2 cut(s) 119, 146
Bsp143I GATC 2 cut(s) 34, 261
BspACI CCGC 5 cut(s) 90, 114, 116, 120, 123
BspANI GGCC 2 cut(s) 119, 146
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 1 cut(s) 221
BspMAI CTGCAG 1 cut(s) 343
BspPI GGATC 1 cut(s) 42
BspT107I GGYRCC 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 1 cut(s) 245
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 2 cut(s) 34, 261
BssNI GRCGYC 2 cut(s) 111, 249
Bst4CI ACNGT 2 cut(s) 43, 195
Bst6I CTCTTC 1 cut(s) 155
BstACI GRCGYC 2 cut(s) 111, 249
BstC8I GCNNGC 3 cut(s) 7, 332, 336
BstDEI CTNAG 1 cut(s) 150
BstDSI CCRYGG 1 cut(s) 114
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 1 cut(s) 292
BstFNI CGCG 1 cut(s) 116
BstKTI GATC 2 cut(s) 37, 264
BstMAI GTCTC 2 cut(s) 17, 256
BstMBI GATC 2 cut(s) 34, 261
BstMCI CGRYCG 1 cut(s) 120
BstMWI GCNNNNNNNGC 4 cut(s) 23, 119, 122, 344
BstSFI CTRYAG 1 cut(s) 339
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 1 cut(s) 325
BstXI CCANNNNNNTGG 1 cut(s) 377
BstZI CGGCCG 1 cut(s) 117
BsuRI GGCC 2 cut(s) 119, 146
BtgI CCRYGG 1 cut(s) 114
BtrI CACGTC 1 cut(s) 63
BtsCI GGATG 1 cut(s) 292
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 2 cut(s) 33, 238
Cac8I GCNNGC 3 cut(s) 7, 332, 336
CaiI CAGNNNCTG 1 cut(s) 449
CciNI GCGGCCGC 1 cut(s) 117
Cfr10I RCCGGY 1 cut(s) 125
Cfr42I CCGCGG 1 cut(s) 117
CseI GACGC 2 cut(s) 119, 238
Csp6I GTAC 2 cut(s) 220, 388
CviAII CATG 3 cut(s) 226, 296, 306
CviQI GTAC 2 cut(s) 220, 388
DdeI CTNAG 1 cut(s) 150
DpnI GATC 2 cut(s) 36, 263
DpnII GATC 2 cut(s) 34, 261
EaeI YGGCCR 2 cut(s) 117, 144
EagI CGGCCG 1 cut(s) 117
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
EclXI CGGCCG 1 cut(s) 117
Eco52I CGGCCG 1 cut(s) 117
Eco57I CTGAAG 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 182
Esp3I CGTCTC 1 cut(s) 256
FaeI CATG 3 cut(s) 229, 299, 309
FaiI YATR 5 cut(s) 227, 297, 307, 321, 431
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 1 cut(s) 225
FatI CATG 3 cut(s) 225, 295, 305
Fnu4HI GCNGC 5 cut(s) 114, 117, 120, 123, 339
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 5 cut(s) 114, 117, 120, 123, 339
GluI GCNGC 5 cut(s) 114, 117, 120, 123, 339
GsuI CTGGAG 1 cut(s) 438
HaeIII GGCC 2 cut(s) 119, 146
HapII CCGG 2 cut(s) 126, 140
HgaI GACGC 2 cut(s) 119, 238
Hin1I GRCGYC 2 cut(s) 111, 249
Hin1II CATG 3 cut(s) 229, 299, 309
HindIII AAGCTT 2 cut(s) 79, 397
HinfI GANTC 2 cut(s) 312, 366
HpaII CCGG 2 cut(s) 126, 140
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 2 cut(s) 77, 261
Hpy8I GTNNAC 1 cut(s) 370
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 1 cut(s) 320
HpyCH4III ACNGT 2 cut(s) 43, 195
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 1 cut(s) 341
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 119, 122, 344
HpyF3I CTNAG 1 cut(s) 150
HpySE526I ACGT 1 cut(s) 62
Hsp92I GRCGYC 2 cut(s) 111, 249
Hsp92II CATG 3 cut(s) 229, 299, 309
KpnI GGTACC 1 cut(s) 223
KspI CCGCGG 1 cut(s) 117
Kzo9I GATC 2 cut(s) 34, 261
LmnI GCTCC 2 cut(s) 352, 457
LpnPI CCDG 7 cut(s) 139, 153, 191, 226, 348, 468, 477
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 1 cut(s) 334
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 1 cut(s) 28
MalI GATC 2 cut(s) 36, 263
MboI GATC 2 cut(s) 34, 261
MboII GAAGA 5 cut(s) 123, 169, 172, 181, 184
MfeI CAATTG 1 cut(s) 205
MlsI TGGCCA 1 cut(s) 146
MluCI AATT 1 cut(s) 205
MluNI TGGCCA 1 cut(s) 146
MlyI GAGTC 2 cut(s) 321, 375
MmeI TCCRAC 1 cut(s) 362
MnlI CCTC 4 cut(s) 48, 147, 178, 378
Mox20I TGGCCA 1 cut(s) 146
MroXI GAANNNNTTC 2 cut(s) 179, 420
MscI TGGCCA 1 cut(s) 146
MslI CAYNNNNRTG 1 cut(s) 375
Msp20I TGGCCA 1 cut(s) 146
MspA1I CMGCKG 2 cut(s) 116, 338
MspI CCGG 2 cut(s) 126, 140
MunI CAATTG 1 cut(s) 205
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 4 cut(s) 23, 119, 122, 344
NdeII GATC 2 cut(s) 34, 261
NlaIII CATG 3 cut(s) 229, 299, 309
NlaIV GGNNCC 1 cut(s) 221
NmuCI GTSAC 1 cut(s) 28
NotI GCGGCCGC 1 cut(s) 117
OliI CACNNNNGTG 1 cut(s) 375
PdmI GAANNNNTTC 2 cut(s) 179, 420
PkrI GCNGC 5 cut(s) 115, 118, 121, 124, 340
PleI GAGTC 2 cut(s) 320, 374
PpsI GAGTC 2 cut(s) 320, 374
PspN4I GGNNCC 1 cut(s) 221
PstI CTGCAG 1 cut(s) 343
PstNI CAGNNNCTG 1 cut(s) 449
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 2 cut(s) 221, 389
RsaNI GTAC 2 cut(s) 220, 388
RseI CAYNNNNRTG 1 cut(s) 375
SacII CCGCGG 1 cut(s) 117
SatI GCNGC 5 cut(s) 114, 117, 120, 123, 339
Sau3AI GATC 2 cut(s) 34, 261
SchI GAGTC 2 cut(s) 321, 375
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 339
Sfr303I CCGCGG 1 cut(s) 117
SgrBI CCGCGG 1 cut(s) 117
SmiMI CAYNNNNRTG 1 cut(s) 375
Sse9I AATT 1 cut(s) 205
SsiI CCGC 5 cut(s) 90, 114, 116, 120, 123
TaaI ACNGT 2 cut(s) 43, 195
TaiI ACGT 1 cut(s) 65
TaqI TCGA 4 cut(s) 37, 65, 183, 410
TasI AATT 1 cut(s) 205
TauI GCSGC 4 cut(s) 116, 119, 122, 125
TscAI CASTG 2 cut(s) 33, 245
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 308
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 2 cut(s) 33, 245
XagI CCTNNNNNAGG 1 cut(s) 182
XmnI GAANNNNTTC 2 cut(s) 179, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.