AT5G62240

Cell cycle regulated microtubule associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006192
Physical Location & Seq
Reverse (-)
25002358 .. 25004783
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62240.1

Sequence Viewer

Length: 1134 bp
ATGGTTCCCGAATCATCATCGAGGATAATGAGTTTTTCTGGCGATGATTTCGAATTCGATATTGATTTCGAATTCGATGCACCGAGGTTCTATGATTTCTCTAGACCTGAGCTTTATTCCGAGACTGAAGAAATCGAAATCTGGTTTCAATCCTCTGGAAACTATCCTCCTTCACCTTTTAGCCCAAAGTTCAATTGGAAATTTGAGCCATTTAAGCAAATTACAAACACCATATCAGAGTCTAAGCCTGTGGAGATTATTGAATCCTCGGAAGCCGAGACCGGTTTGAATTGCAAAGATAAATTCAACGGCTTCATATATTATAACCAAACCGTTAAAGATGTATCCAAGAAAAAATCGAAATCTAAAACCAAAACAAGTAACTCTACTTTGACAAGACCTACGGCTTCTTTGCTTGCAAGGCAGAACAAACCTTTAGATATTTACTCTGTTCAACTTCTGACAAGATGTCAGCGATCATTAGCCAAGTTTGGTGAAAATGTAGCTCCGGTCTTAGTCTCTAAGTTACAAAACCAAGACACCAATAGGCAAAAACAGGAAGCTAAGGTGGCTCATGTTAGCAGCAGAGCCAAACTCACTGTTCCAAAGGAACCGAATCTTAGAACTGCAGAAAGATCTGAAAGACACAGGTCTAAGGTTAACACACAGAGAGAGCAGATTGCGACATCAAATTCTAAAAGACACATTAGGAACAAAAATATTAATCTGGAACCTGTTTCAACATCTTTGGCTAAAACTAATACGCCACGGTCCCAACATTTTCAGGCATTTCGTTTAAGGACATCACTGAGAGCGAAGGAACGCTCTTCAAATGCTAAAACCGATGTCGTACAAGAGAATGATGCTACTAAGTCCAGAACACTAAAAAAGCCCATTGATTCCTTAAAAACTAGGATAGTCAAAGAAAATCACAGCAGAAAGATAAATTGCCAAGTGTATGAATCTAAATCTTCCCCTCTAGACCCAAAGATATCAAGCAAAGAAGACCTCGTTGAAGCAATCCGCATCGAATATGGAAATCAGAGCTCATGCAGGACAGATATTAACAGGAGCCTGGATTTGTGCCGGAAATTCGATTCTCAGGAAGTTGCAAGGAACCTAATCACTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2_importin

Protein Analysis

377

Amino Acids

43.25

Weight (kDa)

9.62

Isoelectric Point (pI)

59.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2_importin PF12214 198 - 331 8e-30 Cell cycle regulated microtubule associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 324
AciI CCGC 1 cut(s) 1024
AcsI RAATTY 6 cut(s) 53, 71, 200, 302, 691, 1091
AcuI CTGAAG 1 cut(s) 147
AfaI GTAC 1 cut(s) 852
AgeI ACCGGT 1 cut(s) 281
AgsI TTSAA 9 cut(s) 149, 193, 263, 289, 307, 455, 741, 831, 1016
AhdI GACNNNNNGTC 1 cut(s) 468
AjnI CCWGG 1 cut(s) 1074
AluBI AGCT 4 cut(s) 112, 506, 563, 1047
AluI AGCT 4 cut(s) 112, 506, 563, 1047
Alw21I GWGCWC 1 cut(s) 1049
Alw26I GTCTC 3 cut(s) 116, 272, 523
ApeKI GCWGC 1 cut(s) 582
ApoI RAATTY 6 cut(s) 53, 71, 200, 302, 691, 1091
AseI ATTAAT 1 cut(s) 723
AsiGI ACCGGT 1 cut(s) 281
AspS9I GGNCC 1 cut(s) 771
AsuHPI GGTGA 2 cut(s) 165, 506
AsuII TTCGAA 2 cut(s) 51, 69
AvaII GGWCC 1 cut(s) 771
BanII GRGCYC 1 cut(s) 1049
BbsI GAAGAC 1 cut(s) 1011
Bbv12I GWGCWC 1 cut(s) 1049
BbvI GCAGC 1 cut(s) 594
BceAI ACGGC 2 cut(s) 325, 420
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BciT130I CCWGG 1 cut(s) 1076
BciVI GTATCC 1 cut(s) 355
BcoDI GTCTC 3 cut(s) 116, 272, 523
BfaI CTAG 3 cut(s) 102, 912, 980
BfmI CTRYAG 1 cut(s) 627
BfuI GTATCC 1 cut(s) 355
BglII AGATCT 1 cut(s) 635
BisI GCNGC 1 cut(s) 583
BlsI GCNGC 1 cut(s) 584
Bme1390I CCNGG 1 cut(s) 1076
Bme18I GGWCC 1 cut(s) 771
BmeRI GACNNNNNGTC 1 cut(s) 468
BmgT120I GGNCC 1 cut(s) 771
BmiI GGNNCC 6 cut(s) 6, 612, 732, 773, 1073, 1118
BmrFI CCNGG 1 cut(s) 1076
BmsI GCATC 3 cut(s) 67, 853, 1035
BoxI GACNNNNGTC 1 cut(s) 649
BpiI GAAGAC 1 cut(s) 1011
BplI GAGNNNNNCTC 2 cut(s) 579, 611
Bpu10I CCTNAGC 2 cut(s) 108, 564
Bpu14I TTCGAA 2 cut(s) 51, 69
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 3 cut(s) 83, 267, 767
BsaWI WCCGGW 2 cut(s) 281, 508
Bse118I RCCGGY 1 cut(s) 281
BseBI CCWGG 1 cut(s) 1076
BseDI CCNNGG 3 cut(s) 83, 267, 767
BseMII CTCAG 3 cut(s) 99, 800, 1115
BseXI GCAGC 1 cut(s) 594
BshTI ACCGGT 1 cut(s) 281
BsiHKAI GWGCWC 1 cut(s) 1049
BsiSI CCGG 3 cut(s) 282, 509, 1087
BslFI GGGAC 1 cut(s) 757
BsmAI GTCTC 3 cut(s) 116, 272, 523
BsmFI GGGAC 1 cut(s) 757
Bso31I GGTCTC 1 cut(s) 272
Bsp119I TTCGAA 2 cut(s) 51, 69
Bsp1286I GDGCHC 1 cut(s) 1049
Bsp143I GATC 2 cut(s) 476, 635
BspACI CCGC 1 cut(s) 1024
BspCNI CTCAG 3 cut(s) 100, 801, 1114
BspLI GGNNCC 6 cut(s) 6, 612, 732, 773, 1073, 1118
BspMAI CTGCAG 1 cut(s) 631
BspQI GCTCTTC 1 cut(s) 832
BspT104I TTCGAA 2 cut(s) 51, 69
BspTNI GGTCTC 1 cut(s) 272
BsrFI RCCGGY 1 cut(s) 281
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 3 cut(s) 83, 267, 767
BssMI GATC 2 cut(s) 476, 635
Bst2UI CCWGG 1 cut(s) 1076
Bst4CI ACNGT 3 cut(s) 334, 601, 771
Bst6I CTCTTC 1 cut(s) 832
BstBI TTCGAA 2 cut(s) 51, 69
BstC8I GCNNGC 1 cut(s) 417
BstDSI CCRYGG 1 cut(s) 767
BstKTI GATC 2 cut(s) 479, 638
BstMAI GTCTC 3 cut(s) 116, 272, 523
BstMBI GATC 2 cut(s) 476, 635
BstMWI GCNNNNNNNGC 3 cut(s) 214, 421, 569
BstNI CCWGG 1 cut(s) 1076
BstPAI GACNNNNGTC 1 cut(s) 649
BstSCI CCNGG 1 cut(s) 1074
BstSFI CTRYAG 1 cut(s) 627
BstV1I GCAGC 1 cut(s) 594
BstV2I GAAGAC 1 cut(s) 1011
BstX2I RGATCY 1 cut(s) 635
BstYI RGATCY 1 cut(s) 635
BsuI GTATCC 1 cut(s) 355
BtgI CCRYGG 1 cut(s) 767
BtgZI GCGATG 1 cut(s) 57
BtsI GCAGTG 1 cut(s) 1125
BtsIMutI CAGTG 3 cut(s) 597, 806, 1125
Cac8I GCNNGC 1 cut(s) 417
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 1 cut(s) 771
Csp6I GTAC 1 cut(s) 851
CspAI ACCGGT 1 cut(s) 281
CviAII CATG 2 cut(s) 575, 1050
CviQI GTAC 1 cut(s) 851
DpnI GATC 2 cut(s) 478, 637
DpnII GATC 2 cut(s) 476, 635
DriI GACNNNNNGTC 1 cut(s) 468
Eam1104I CTCTTC 1 cut(s) 832
Eam1105I GACNNNNNGTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 832
Ecl136II GAGCTC 1 cut(s) 1047
Eco24I GRGCYC 1 cut(s) 1049
Eco31I GGTCTC 1 cut(s) 272
Eco32I GATATC 1 cut(s) 993
Eco47I GGWCC 1 cut(s) 771
Eco53kI GAGCTC 1 cut(s) 1047
Eco57I CTGAAG 1 cut(s) 147
EcoICRI GAGCTC 1 cut(s) 1047
EcoRI GAATTC 2 cut(s) 53, 71
EcoRII CCWGG 1 cut(s) 1074
EcoRV GATATC 1 cut(s) 993
EcoT38I GRGCYC 1 cut(s) 1049
FaeI CATG 2 cut(s) 578, 1053
FaiI YATR 9 cut(s) 93, 233, 317, 319, 324, 576, 960, 1035, 1051
FaqI GGGAC 1 cut(s) 757
FatI CATG 2 cut(s) 574, 1049
Fnu4HI GCNGC 1 cut(s) 583
FriOI GRGCYC 1 cut(s) 1049
Fsp4HI GCNGC 1 cut(s) 583
FspBI CTAG 3 cut(s) 102, 912, 980
GluI GCNGC 1 cut(s) 583
HapII CCGG 3 cut(s) 282, 509, 1087
Hin1II CATG 2 cut(s) 578, 1053
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HinfI GANTC 7 cut(s) 11, 239, 263, 616, 899, 962, 1097
HpaI GTTAAC 1 cut(s) 661
HpaII CCGG 3 cut(s) 282, 509, 1087
HphI GGTGA 2 cut(s) 165, 506
Hpy166II GTNNAC 1 cut(s) 661
Hpy188I TCNGA 6 cut(s) 121, 238, 271, 462, 640, 1044
Hpy188III TCNNGA 7 cut(s) 8, 102, 156, 728, 876, 980, 1103
Hpy8I GTNNAC 1 cut(s) 661
HpyAV CCTTC 2 cut(s) 180, 811
HpyCH4III ACNGT 3 cut(s) 334, 601, 771
HpyCH4V TGCA 6 cut(s) 80, 294, 419, 629, 1053, 1112
HpyF10VI GCNNNNNNNGC 3 cut(s) 214, 421, 569
Hsp92II CATG 2 cut(s) 578, 1053
KspAI GTTAAC 1 cut(s) 661
Kzo9I GATC 2 cut(s) 476, 635
LguI GCTCTTC 1 cut(s) 832
LmnI GCTCC 2 cut(s) 511, 1071
Lsp1109I GCAGC 1 cut(s) 594
LweI GCATC 3 cut(s) 67, 853, 1035
MaeI CTAG 3 cut(s) 102, 912, 980
MaeIII GTNAC 2 cut(s) 380, 525
MalI GATC 2 cut(s) 478, 637
MboI GATC 2 cut(s) 476, 635
MboII GAAGA 4 cut(s) 140, 819, 963, 1016
MfeI CAATTG 1 cut(s) 193
MflI RGATCY 1 cut(s) 635
MhlI GDGCHC 1 cut(s) 1049
MlyI GAGTC 1 cut(s) 248
MnlI CCTC 7 cut(s) 15, 78, 163, 177, 277, 987, 1019
MseI TTAA 8 cut(s) 213, 336, 660, 723, 797, 905, 1065, 1132
MspI CCGG 3 cut(s) 282, 509, 1087
MspR9I CCNGG 1 cut(s) 1076
MunI CAATTG 1 cut(s) 193
MvaI CCWGG 1 cut(s) 1076
MwoI GCNNNNNNNGC 3 cut(s) 214, 421, 569
NdeII GATC 2 cut(s) 476, 635
NlaIII CATG 2 cut(s) 578, 1053
NlaIV GGNNCC 6 cut(s) 6, 612, 732, 773, 1073, 1118
NmeAIII GCCGAG 1 cut(s) 301
NspV TTCGAA 2 cut(s) 51, 69
PciSI GCTCTTC 1 cut(s) 832
PfeI GAWTC 6 cut(s) 11, 263, 616, 899, 962, 1097
PinAI ACCGGT 1 cut(s) 281
PkrI GCNGC 1 cut(s) 584
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PshAI GACNNNNGTC 1 cut(s) 649
PshBI ATTAAT 1 cut(s) 723
PsiI TTATAA 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 1049
Psp6I CCWGG 1 cut(s) 1074
PspGI CCWGG 1 cut(s) 1074
PspN4I GGNNCC 6 cut(s) 6, 612, 732, 773, 1073, 1118
PspPI GGNCC 1 cut(s) 771
PstI CTGCAG 1 cut(s) 631
PsuI RGATCY 1 cut(s) 635
RsaI GTAC 1 cut(s) 852
RsaNI GTAC 1 cut(s) 851
SacI GAGCTC 1 cut(s) 1049
SapI GCTCTTC 1 cut(s) 832
SaqAI TTAA 8 cut(s) 213, 336, 660, 723, 797, 905, 1065, 1132
SatI GCNGC 1 cut(s) 583
Sau3AI GATC 2 cut(s) 476, 635
Sau96I GGNCC 1 cut(s) 771
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 1 cut(s) 1076
SduI GDGCHC 1 cut(s) 1049
SfaNI GCATC 3 cut(s) 67, 853, 1035
SfcI CTRYAG 1 cut(s) 627
SfuI TTCGAA 2 cut(s) 51, 69
SinI GGWCC 1 cut(s) 771
SsiI CCGC 1 cut(s) 1024
SspI AATATT 1 cut(s) 721
SspMI CTAG 3 cut(s) 102, 912, 980
SstI GAGCTC 1 cut(s) 1049
StyD4I CCNGG 1 cut(s) 1074
TaaI ACNGT 3 cut(s) 334, 601, 771
TaqI TCGA 9 cut(s) 20, 51, 57, 69, 75, 135, 359, 1029, 1095
TfiI GAWTC 6 cut(s) 11, 263, 616, 899, 962, 1097
Tru1I TTAA 8 cut(s) 213, 336, 660, 723, 797, 905, 1065, 1132
Tru9I TTAA 8 cut(s) 213, 336, 660, 723, 797, 905, 1065, 1132
TscAI CASTG 3 cut(s) 604, 813, 1132
TseI GCWGC 1 cut(s) 582
TspDTI ATGAA 2 cut(s) 304, 975
TspRI CASTG 3 cut(s) 604, 813, 1132
VpaK11BI GGWCC 1 cut(s) 771
VspI ATTAAT 1 cut(s) 723
XapI RAATTY 6 cut(s) 53, 71, 200, 302, 691, 1091
XbaI TCTAGA 2 cut(s) 101, 979
XcmI CCANNNNNNNNNTGG 1 cut(s) 192
XspI CTAG 3 cut(s) 102, 912, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.