AT5G63290

Radical S-adenosyl methionine domain-containing protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009349
Physical Location & Seq
Reverse (-)
25369245 .. 25370940
1696 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G63290.1

Sequence Viewer

Length: 1455 bp
ATGCTGAAAACAACGATTTCTCCAATTTTCTCATCGTTTACAGGTAAACCCAAGTGCTCATCGAAGTTGTTTTTTCGAGCTTTTAGTAAGGTTGTTCTACAAGATACTCCACCAAGTGCTCGACGAAATGCGTCAACCAATCTTACTACGTTACACAAAGGTCCACCAACCTCAGCCTACGTTCATCTTCCGTTTTGTCGCAAACGCTGTCATTATTGTGACTTTCCTATCTTAGCTCTAGGCATGTCTTCTTCTTCAGCCAGACCGTCTAATGTCTACGAGGAAGGAAAAGAAGATGACCCGAGAATCACGAACTATGTGAATTTGCTTGTTAGAGAGATAAAAGCGACAAGGACGGATTTTGATACGAACCCGAATCTAGAAACGGTGTTTTTCGGTGGTGGGACGCCTTCTCTTGTTCCTCCGAAGCTTGTTTCTTTGATTCTGGAAACATTGAGTTTGAATTTCGGGCTGAGTCCAGATGCTGAGATATCAATGGAGATGGATCCAGGCACGTTTGATGGTCAGAAGCTGAAGGATTTGATGAAATTGGGAGTTAACAGAGTGTCTTTAGGAGTTCAAGCTTTTCAAGATGAGCTTTTGAAAGCTTGTGGAAGAGCTCATGGTGTCTCTCAAGTGTATGAAGCAATAGAGTTTGTTAAAGAATGTGGAGTTGAGAATTGGAGTATGGATCTTATTTCTTCTCTGCCTCACCAGACTTTGGAGATGTGGGAAGAGAGTTTGAGATTAGCTATCGAATCACAGCCCAATCATGTTTCTGTATATGATCTGCAAGTCGAACAAGGCACAAAGTTTGGAAATTTGTACACTCCAGGACAGTCCCCTCTTCCTTCAGAAACACAGTCTGCAGAGTTTTATAAAACAGCATCATCGATGCTCCGTGGTGCAGGTTATGAACATTATGAAGTCAGCAGTTACTCGAGAGACGGTTTCAAGTGCAAACACAACTTGATATACTGGAAGAACAAGCCATTCTATGCTTTCGGTTTAGGTTCAGCGAGTTATGTTGGAGGGTTGAGGTTTTCAAGGCCAAGGAGGCTAAAGGAATACACAAACTATGTCGCTGACTTGGAGAATGGCGCAGCAAACTGGTGTGGAAATGGTGATGTTGATCTTAAGGATGTTGCAACAGACATCTTAATGCTATCTTTCAGAACATCTAAAGGGCTTGAACTTAAGGAGTTTGGAGAAGCTTTTGGCAGTGAGGTAGTGAAATCAATCTGTAAAGTTTATGAGCCTTATGTGGAGAGTGGGCACATTGTTTGTTTGGATGATATGAGAAGCGAAGTGATGATTGATGAATTCAAAACCTTAGTTGCAAATGATGAAGTGAAGATTGAGGATCATGTGAGGTACCTTAGACTGAAAGATCCAGATGGTTTTCTCCTCTCTAATGAGTTGATATCTCTATCGTTTGGCGTGGTAGCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HemN_C Radical_SAM

Protein Analysis

484

Amino Acids

54.05

Weight (kDa)

5.69

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 60 - 251 3.9e-27 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 879
Acc36I ACCTGC 1 cut(s) 899
Acc65I GGTACC 1 cut(s) 1374
AccB1I GGYRCC 1 cut(s) 1374
AccB7I CCANNNNNTGG 1 cut(s) 721
AccI GTMKAC 1 cut(s) 276
AclWI GGATC 5 cut(s) 500, 513, 699, 1371, 1385
AcsI RAATTY 4 cut(s) 322, 463, 820, 1322
AcuI CTGAAG 3 cut(s) 240, 554, 837
AcyI GRCGYC 1 cut(s) 407
AdeI CACNNNGTG 1 cut(s) 116
AfaI GTAC 2 cut(s) 827, 1376
AfiI CCNNNNNNNGG 1 cut(s) 721
AflII CTTAAG 2 cut(s) 1136, 1196
AgsI TTSAA 8 cut(s) 463, 581, 590, 604, 955, 1047, 1193, 1327
AjnI CCWGG 2 cut(s) 508, 832
Alw21I GWGCWC 3 cut(s) 59, 121, 622
Alw26I GTCTC 2 cut(s) 634, 939
AlwI GGATC 5 cut(s) 500, 513, 699, 1371, 1385
AlwNI CAGNNNCTG 2 cut(s) 485, 532
Ama87I CYCGRG 2 cut(s) 301, 940
AoxI GGCC 1 cut(s) 1049
ApeKI GCWGC 1 cut(s) 1103
ApoI RAATTY 4 cut(s) 322, 463, 820, 1322
Asp718I GGTACC 1 cut(s) 1374
AspLEI GCGC 1 cut(s) 1103
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 704, 1136
AvaI CYCGRG 2 cut(s) 301, 940
AvaII GGWCC 1 cut(s) 161
BaeGI GKGCMC 1 cut(s) 1278
BamHI GGATCC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 1374
BanII GRGCYC 1 cut(s) 622
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 3 cut(s) 59, 121, 622
BbvCI CCTCAGC 1 cut(s) 172
BbvI GCAGC 1 cut(s) 1115
BccI CCATC 3 cut(s) 496, 515, 1391
BciT130I CCWGG 2 cut(s) 510, 834
BcoDI GTCTC 2 cut(s) 634, 939
BfaI CTAG 2 cut(s) 239, 380
BfmI CTRYAG 1 cut(s) 867
BfrI CTTAAG 2 cut(s) 1136, 1196
BfuAI ACCTGC 1 cut(s) 899
BglI GCCNNNNNGGC 1 cut(s) 1057
BisI GCNGC 1 cut(s) 1104
BlsI GCNGC 1 cut(s) 1105
Bme1390I CCNGG 2 cut(s) 510, 834
Bme18I GGWCC 1 cut(s) 161
BmeT110I CYCGRG 2 cut(s) 301, 940
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 2 cut(s) 507, 1376
BmrFI CCNGG 2 cut(s) 510, 834
BmsI GCATC 3 cut(s) 472, 885, 896
BpiI GAAGAC 1 cut(s) 240
BpmI CTGGAG 1 cut(s) 816
Bpu10I CCTNAGC 1 cut(s) 172
BpuEI CTTGAG 1 cut(s) 618
Bsa29I ATCGAT 1 cut(s) 893
BsaBI GATNNNNATC 1 cut(s) 1131
BsaHI GRCGYC 1 cut(s) 407
BsaJI CCNNGG 2 cut(s) 901, 1052
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 1 cut(s) 721
Bse1I ACTGG 2 cut(s) 983, 1115
Bse8I GATNNNNATC 1 cut(s) 1131
BseBI CCWGG 2 cut(s) 510, 834
BseCI ATCGAT 1 cut(s) 893
BseDI CCNNGG 2 cut(s) 901, 1052
BseGI GGATG 2 cut(s) 1147, 1297
BseJI GATNNNNATC 1 cut(s) 1131
BseLI CCNNNNNNNGG 1 cut(s) 721
BseMII CTCAG 3 cut(s) 186, 464, 477
BseNI ACTGG 2 cut(s) 983, 1115
BseRI GAGGAG 1 cut(s) 1397
BseSI GKGCMC 1 cut(s) 1278
BseXI GCAGC 1 cut(s) 1115
BsgI GTGCAG 1 cut(s) 927
BshFI GGCC 1 cut(s) 1051
BshNI GGYRCC 1 cut(s) 1374
BshVI ATCGAT 1 cut(s) 893
BsiHKAI GWGCWC 3 cut(s) 59, 121, 622
BsiHKCI CYCGRG 2 cut(s) 301, 940
BslFI GGGAC 2 cut(s) 418, 826
BslI CCNNNNNNNGG 1 cut(s) 721
BsmAI GTCTC 2 cut(s) 634, 939
BsmBI CGTCTC 1 cut(s) 939
BsmFI GGGAC 2 cut(s) 418, 826
BsnI GGCC 1 cut(s) 1051
BsoBI CYCGRG 2 cut(s) 301, 940
Bsp1286I GDGCHC 4 cut(s) 59, 121, 622, 1278
Bsp1407I TGTACA 1 cut(s) 825
Bsp143I GATC 6 cut(s) 505, 691, 787, 1132, 1363, 1390
BspANI GGCC 1 cut(s) 1051
BspCNI CTCAG 3 cut(s) 185, 465, 478
BspDI ATCGAT 1 cut(s) 893
BspLI GGNNCC 2 cut(s) 507, 1376
BspMAI CTGCAG 1 cut(s) 871
BspMI ACCTGC 1 cut(s) 899
BspPI GGATC 5 cut(s) 500, 513, 699, 1371, 1385
BspQI GCTCTTC 1 cut(s) 610
BspT107I GGYRCC 1 cut(s) 1374
BspTI CTTAAG 2 cut(s) 1136, 1196
BsrGI TGTACA 1 cut(s) 825
BsrI ACTGG 2 cut(s) 983, 1115
BssECI CCNNGG 2 cut(s) 901, 1052
BssMI GATC 6 cut(s) 505, 691, 787, 1132, 1363, 1390
BssNI GRCGYC 1 cut(s) 407
BssT1I CCWWGG 1 cut(s) 1052
Bst2UI CCWGG 2 cut(s) 510, 834
Bst4CI ACNGT 5 cut(s) 267, 388, 840, 864, 950
Bst6I CTCTTC 3 cut(s) 610, 729, 852
BstACI GRCGYC 1 cut(s) 407
BstAFI CTTAAG 2 cut(s) 1136, 1196
BstAUI TGTACA 1 cut(s) 825
BstDEI CTNAG 6 cut(s) 172, 232, 473, 486, 1333, 1379
BstDSI CCRYGG 1 cut(s) 901
BstF5I GGATG 2 cut(s) 1147, 1297
BstHHI GCGC 1 cut(s) 1103
BstKTI GATC 6 cut(s) 508, 694, 790, 1135, 1366, 1393
BstMAI GTCTC 2 cut(s) 634, 939
BstMBI GATC 6 cut(s) 505, 691, 787, 1132, 1363, 1390
BstMWI GCNNNNNNNGC 1 cut(s) 1057
BstNI CCWGG 2 cut(s) 510, 834
BstNSI RCATGY 1 cut(s) 247
BstSCI CCNGG 2 cut(s) 508, 832
BstSFI CTRYAG 1 cut(s) 867
BstSLI GKGCMC 1 cut(s) 1278
BstV1I GCAGC 1 cut(s) 1115
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 3 cut(s) 505, 691, 1390
BstYI RGATCY 3 cut(s) 505, 691, 1390
Bsu15I ATCGAT 1 cut(s) 893
BsuRI GGCC 1 cut(s) 1051
BsuTUI ATCGAT 1 cut(s) 893
BtgI CCRYGG 1 cut(s) 901
BtsCI GGATG 2 cut(s) 1147, 1297
BtsI GCAGTG 1 cut(s) 1228
BtsIMutI CAGTG 1 cut(s) 1228
BveI ACCTGC 1 cut(s) 899
CaiI CAGNNNCTG 2 cut(s) 485, 532
CfoI GCGC 1 cut(s) 1103
Cfr13I GGNCC 1 cut(s) 161
ClaI ATCGAT 1 cut(s) 893
CseI GACGC 2 cut(s) 120, 415
Csp6I GTAC 2 cut(s) 826, 1375
CviAII CATG 4 cut(s) 244, 623, 773, 1367
CviQI GTAC 2 cut(s) 826, 1375
DdeI CTNAG 6 cut(s) 172, 232, 473, 486, 1333, 1379
DpnI GATC 6 cut(s) 507, 693, 789, 1134, 1365, 1392
DpnII GATC 6 cut(s) 505, 691, 787, 1132, 1363, 1390
DraIII CACNNNGTG 1 cut(s) 116
Eam1104I CTCTTC 3 cut(s) 610, 729, 852
EarI CTCTTC 3 cut(s) 610, 729, 852
Ecl136II GAGCTC 1 cut(s) 620
Eco130I CCWWGG 1 cut(s) 1052
Eco24I GRGCYC 1 cut(s) 622
Eco32I GATATC 2 cut(s) 492, 1425
Eco47I GGWCC 1 cut(s) 161
Eco53kI GAGCTC 1 cut(s) 620
Eco57I CTGAAG 3 cut(s) 240, 554, 837
Eco88I CYCGRG 2 cut(s) 301, 940
EcoICRI GAGCTC 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 1322
EcoRII CCWGG 2 cut(s) 508, 832
EcoRV GATATC 2 cut(s) 492, 1425
EcoT14I CCWWGG 1 cut(s) 1052
EcoT38I GRGCYC 1 cut(s) 622
ErhI CCWWGG 1 cut(s) 1052
Esp3I CGTCTC 1 cut(s) 939
FaeI CATG 4 cut(s) 247, 626, 776, 1370
FalI AAGNNNNNCTT 2 cut(s) 582, 614
FaqI GGGAC 2 cut(s) 418, 826
FatI CATG 4 cut(s) 243, 622, 772, 1366
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 1 cut(s) 1104
FokI GGATG 2 cut(s) 1154, 1304
FriOI GRGCYC 1 cut(s) 622
Fsp4HI GCNGC 1 cut(s) 1104
FspBI CTAG 2 cut(s) 239, 380
GlaI GCGC 1 cut(s) 1102
GluI GCNGC 1 cut(s) 1104
GsuI CTGGAG 1 cut(s) 816
HaeIII GGCC 1 cut(s) 1051
HgaI GACGC 2 cut(s) 120, 415
HhaI GCGC 1 cut(s) 1103
Hin1I GRCGYC 1 cut(s) 407
Hin1II CATG 4 cut(s) 247, 626, 776, 1370
Hin6I GCGC 1 cut(s) 1101
HinP1I GCGC 1 cut(s) 1101
HincII GTYRAC 2 cut(s) 135, 559
HindII GTYRAC 2 cut(s) 135, 559
HindIII AAGCTT 4 cut(s) 428, 582, 606, 1212
HinfI GANTC 5 cut(s) 306, 376, 442, 475, 758
HpaI GTTAAC 1 cut(s) 559
HphI GGTGA 2 cut(s) 704, 1136
Hpy166II GTNNAC 7 cut(s) 39, 47, 135, 164, 277, 559, 828
Hpy188I TCNGA 4 cut(s) 426, 528, 856, 1175
Hpy188III TCNNGA 7 cut(s) 310, 380, 446, 479, 590, 942, 1394
Hpy8I GTNNAC 7 cut(s) 39, 47, 135, 164, 277, 559, 828
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 4 cut(s) 278, 420, 529, 861
HpyCH4III ACNGT 5 cut(s) 267, 388, 840, 864, 950
HpyCH4IV ACGT 3 cut(s) 149, 180, 515
HpyCH4V TGCA 6 cut(s) 793, 869, 908, 960, 1148, 1340
HpyF10VI GCNNNNNNNGC 1 cut(s) 1057
HpyF3I CTNAG 6 cut(s) 172, 232, 473, 486, 1333, 1379
HpySE526I ACGT 3 cut(s) 149, 180, 515
Hsp92I GRCGYC 1 cut(s) 407
Hsp92II CATG 4 cut(s) 247, 626, 776, 1370
HspAI GCGC 1 cut(s) 1101
KpnI GGTACC 1 cut(s) 1378
KspAI GTTAAC 1 cut(s) 559
Kzo9I GATC 6 cut(s) 505, 691, 787, 1132, 1363, 1390
LguI GCTCTTC 1 cut(s) 610
LmnI GCTCC 2 cut(s) 903, 1453
Lsp1109I GCAGC 1 cut(s) 1115
LweI GCATC 3 cut(s) 472, 885, 896
MaeI CTAG 2 cut(s) 239, 380
MaeII ACGT 3 cut(s) 149, 180, 515
MaeIII GTNAC 3 cut(s) 150, 218, 935
MalI GATC 6 cut(s) 507, 693, 789, 1134, 1365, 1392
MboI GATC 6 cut(s) 505, 691, 787, 1132, 1363, 1390
MflI RGATCY 3 cut(s) 505, 691, 1390
MhlI GDGCHC 4 cut(s) 59, 121, 622, 1278
MluCI AATT 7 cut(s) 24, 322, 463, 548, 679, 820, 1322
MlyI GAGTC 1 cut(s) 484
MmeI TCCRAC 1 cut(s) 1009
MseI TTAA 6 cut(s) 558, 660, 1137, 1160, 1197, 1453
MslI CAYNNNNRTG 2 cut(s) 216, 1160
MspCI CTTAAG 2 cut(s) 1136, 1196
MspR9I CCNGG 2 cut(s) 510, 834
MvaI CCWGG 2 cut(s) 510, 834
MwoI GCNNNNNNNGC 1 cut(s) 1057
NdeII GATC 6 cut(s) 505, 691, 787, 1132, 1363, 1390
NlaIII CATG 4 cut(s) 247, 626, 776, 1370
NlaIV GGNNCC 2 cut(s) 507, 1376
NmuCI GTSAC 1 cut(s) 218
NspI RCATGY 1 cut(s) 247
PaeR7I CTCGAG 1 cut(s) 940
PciSI GCTCTTC 1 cut(s) 610
PfeI GAWTC 4 cut(s) 306, 376, 442, 758
PflMI CCANNNNNTGG 1 cut(s) 721
PfoI TCCNGGA 1 cut(s) 832
PkrI GCNGC 1 cut(s) 1105
PleI GAGTC 1 cut(s) 483
PpsI GAGTC 1 cut(s) 483
PsiI TTATAA 1 cut(s) 879
Psp124BI GAGCTC 1 cut(s) 622
Psp6I CCWGG 2 cut(s) 508, 832
PspGI CCWGG 2 cut(s) 508, 832
PspN4I GGNNCC 2 cut(s) 507, 1376
PspPI GGNCC 1 cut(s) 161
PstI CTGCAG 1 cut(s) 871
PstNI CAGNNNCTG 2 cut(s) 485, 532
PsuI RGATCY 3 cut(s) 505, 691, 1390
RsaI GTAC 2 cut(s) 827, 1376
RsaNI GTAC 2 cut(s) 826, 1375
RseI CAYNNNNRTG 2 cut(s) 216, 1160
SacI GAGCTC 1 cut(s) 622
SapI GCTCTTC 1 cut(s) 610
SaqAI TTAA 6 cut(s) 558, 660, 1137, 1160, 1197, 1453
SatI GCNGC 1 cut(s) 1104
Sau3AI GATC 6 cut(s) 505, 691, 787, 1132, 1363, 1390
Sau96I GGNCC 1 cut(s) 161
SchI GAGTC 1 cut(s) 484
ScrFI CCNGG 2 cut(s) 510, 834
SduI GDGCHC 4 cut(s) 59, 121, 622, 1278
SfaNI GCATC 3 cut(s) 472, 885, 896
SfcI CTRYAG 1 cut(s) 867
Sfr274I CTCGAG 1 cut(s) 940
SinI GGWCC 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 940
SmiMI CAYNNNNRTG 2 cut(s) 216, 1160
SmlI CTYRAG 4 cut(s) 633, 940, 1136, 1196
SmoI CTYRAG 4 cut(s) 633, 940, 1136, 1196
Sse9I AATT 7 cut(s) 24, 322, 463, 548, 679, 820, 1322
SspMI CTAG 2 cut(s) 239, 380
SstI GAGCTC 1 cut(s) 622
StyD4I CCNGG 2 cut(s) 508, 832
StyI CCWWGG 1 cut(s) 1052
TaaI ACNGT 5 cut(s) 267, 388, 840, 864, 950
TaiI ACGT 3 cut(s) 152, 183, 518
TaqI TCGA 7 cut(s) 62, 76, 121, 756, 798, 893, 941
TasI AATT 7 cut(s) 24, 322, 463, 548, 679, 820, 1322
TatI WGTACW 1 cut(s) 825
TfiI GAWTC 4 cut(s) 306, 376, 442, 758
Tru1I TTAA 6 cut(s) 558, 660, 1137, 1160, 1197, 1453
Tru9I TTAA 6 cut(s) 558, 660, 1137, 1160, 1197, 1453
TscAI CASTG 1 cut(s) 1228
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 1 cut(s) 1103
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 7 cut(s) 173, 560, 657, 930, 939, 1335, 1362
TspGWI ACGGA 3 cut(s) 180, 371, 890
TspRI CASTG 1 cut(s) 1228
Van91I CCANNNNNTGG 1 cut(s) 721
Vha464I CTTAAG 2 cut(s) 1136, 1196
VpaK11BI GGWCC 1 cut(s) 161
XapI RAATTY 4 cut(s) 322, 463, 820, 1322
XbaI TCTAGA 1 cut(s) 379
XceI RCATGY 1 cut(s) 247
XhoI CTCGAG 1 cut(s) 940
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 2 cut(s) 239, 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.