MD14G1163800.v1.1

Oxygen-independent coproporphyrinogen-III oxidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009349
Physical Location & Seq
Forward (+)
25782522 .. 25784250
1729 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1163800.v1.1.491

Sequence Viewer

Length: 1416 bp
ATGCTTAAATCAAGCTTCGCTCCCATTTTCTCAACCTTTCCCGCCAAACGCCTATCGCCGCCGAAGCCTCCGCCGGGCACTATCTTCACCTGCTTCGCACACACTGCGCCAACTGTTCGACAAAATGCCTCAACCAAAACCCCCGCCACCATTCTCCCGCTTCCCACTTCCGCCTACATCCACCTCCCTTTCTGCCGGAAGCGCTGCCACTACTGCGACTTCACAATCGTTGCCCTCGGCTCTGCTTCCACCCAAACGGACGATGACCCGCGAATGACGAACTACGTCCAGTTACTCTGCCGAGAAATCAACGCCACCAAAGCCGAAAACAGGACCAACCCGCCTCTGGAGACGGTGTTTTTTGGCGGCGGCACGCCGTCTCTTGTGCCGCCAAAGCTCGTTAATTCCGTTTTGGAGACGTTGAGGTTGAAATTCGGGTTGAGTTCGGAGGCTGAGGTGTCAATGGAGATGGACCCGGGGACTTTCGATGCGAAGAAGATGAGGGGAATGATGGAGCTGGGTGTGAATAGGGTGTCATTGGGAGTTCAGGCGTTTCAGGAAGAGCTGTTGAAAGCTTGTGGGAGAGCTCATGGTCTTAGGGAAGTCTACGAGGCTATGGAGATTGTTGGGGAATGTGGGGTTGAGAATTGGAGCGTGGATCTTATCTCTTCTCTGCCTCACCAGACCCCGGAAATGTGGGAGGAGAGCTTGAGGCTGACGGTTGAAGCTCGTCCAACCCATGTTTCGGTTTATGATTTGCAAGTCGAACAAGGCACAAAATTCGGGTTGTTGTACACACCAGGGGAGTTCCCGTTGCCTTCAGAAACACGGTCAGCTGAGTTTTATAAGATGGCGTCTAGGATGCTTTCCGATGCAGGTTACAACCACTACGAAATTAGCAGTTACTGCAAGAGCGGATATCACTGCAGACACAATCTCACATATTGGAAGAACAAACCTTTCTATGCGTTTGGCCTCGGCTCTGCTAGCCATGTCGATGGTGTGAGGTTTTCAAGACCGAAGAGGATGAAAGAGTACACAGGTTATGTGGAGAATTTGGAAAATGGATTGGTGGATCGCTGCGAAAGTAATCATACTGCACAGGATATGGCCACGGATGTTGTTATGCTCTCTCTTAGAACTTCAAGAGGTCTGGATTTGAAGTCTTTCGGAGAAGCATATGGGAGCTCTCTCGTTCTTTCTCTTTGCAAGGCCTATAAACCTTATGTTGAAAGTGGGCATGTGGTTTTCTTGGATGAACAGAGAAGGGCCATCACTGCAGATGAATTTGACGCCTTGCAAGTAAACAAGGACGAGACTGAAAGAAGTCCTGCCTACATTCGGCTTAGTGATCCAGACGGTTTCCTTTTGTCGAATGAATTGATATCGCTTGCATTTGCAGTTGTAGCTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HemN_C Radical_SAM

Protein Analysis

472

Amino Acids

52.51

Weight (kDa)

6.1

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 59 - 239 1.6e-25 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 846
AarI CACCTGC 1 cut(s) 98
Acc36I ACCTGC 2 cut(s) 98, 866
AccBSI CCGCTC 1 cut(s) 915
AccI GTMKAC 1 cut(s) 606
AccII CGCG 1 cut(s) 271
AclWI GGATC 3 cut(s) 666, 1083, 1346
AcoI YGGCCR 1 cut(s) 1110
AcsI RAATTY 4 cut(s) 431, 779, 1054, 1286
AcuI CTGAAG 1 cut(s) 804
AcyI GRCGYC 2 cut(s) 854, 1293
AfaI GTAC 2 cut(s) 794, 1037
AfeI AGCGCT 1 cut(s) 203
AfiI CCNNNNNNNGG 6 cut(s) 74, 330, 346, 688, 745, 1341
AgsI TTSAA 7 cut(s) 430, 571, 725, 1014, 1146, 1162, 1232
AjnI CCWGG 1 cut(s) 799
AjuI GAANNNNNNNTTGG 2 cut(s) 727, 759
Alw21I GWGCWC 2 cut(s) 589, 1190
Alw26I GTCTC 4 cut(s) 344, 384, 410, 1310
AlwI GGATC 3 cut(s) 666, 1083, 1346
AlwNI CAGNNNCTG 1 cut(s) 906
Ama87I CYCGRG 1 cut(s) 475
Aor51HI AGCGCT 1 cut(s) 203
AoxI GGCC 4 cut(s) 973, 1110, 1212, 1269
ApeKI GCWGC 2 cut(s) 204, 1080
ApoI RAATTY 4 cut(s) 431, 779, 1054, 1286
Asp700I GAANNNNTTC 1 cut(s) 1166
AspLEI GCGC 2 cut(s) 109, 204
AspS9I GGNCC 3 cut(s) 333, 472, 1269
AsuC2I CCSGG 4 cut(s) 75, 476, 477, 689
AsuHPI GGTGA 2 cut(s) 79, 671
AsuNHI GCTAGC 1 cut(s) 986
AvaI CYCGRG 1 cut(s) 475
AvaII GGWCC 2 cut(s) 333, 472
BaeGI GKGCMC 1 cut(s) 80
BalI TGGCCA 1 cut(s) 1112
BanII GRGCYC 2 cut(s) 589, 1190
Bbv12I GWGCWC 2 cut(s) 589, 1190
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 2 cut(s) 191, 1067
BccI CCATC 5 cut(s) 463, 505, 844, 992, 1280
BceAI ACGGC 1 cut(s) 361
BcgI CGANNNNNNTGC 2 cut(s) 763, 797
BciT130I CCWGG 1 cut(s) 801
BcnI CCSGG 4 cut(s) 75, 476, 477, 689
BcoDI GTCTC 4 cut(s) 344, 384, 410, 1310
BfaI CTAG 2 cut(s) 858, 987
BfmI CTRYAG 2 cut(s) 925, 1278
BfoI RGCGCY 1 cut(s) 205
BfuAI ACCTGC 2 cut(s) 98, 866
BisI GCNGC 6 cut(s) 59, 205, 367, 370, 389, 1081
BlsI GCNGC 6 cut(s) 60, 206, 368, 371, 390, 1082
Bme1390I CCNGG 5 cut(s) 75, 476, 477, 689, 801
Bme18I GGWCC 2 cut(s) 333, 472
BmeT110I CYCGRG 1 cut(s) 475
BmgT120I GGNCC 3 cut(s) 333, 472, 1269
BmiI GGNNCC 1 cut(s) 474
BmrFI CCNGG 5 cut(s) 75, 476, 477, 689, 801
BmsI GCATC 3 cut(s) 478, 852, 862
BmtI GCTAGC 1 cut(s) 990
BpmI CTGGAG 1 cut(s) 368
Bpu10I CCTNAGC 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 730
BpuMI CCSGG 4 cut(s) 75, 476, 477, 689
BsaHI GRCGYC 2 cut(s) 854, 1293
BsaJI CCNNGG 7 cut(s) 235, 475, 476, 687, 800, 976, 1113
BsaXI ACNNNNNCTCC 4 cut(s) 341, 371, 797, 827
Bsc4I CCNNNNNNNGG 6 cut(s) 74, 330, 346, 688, 745, 1341
Bse1I ACTGG 1 cut(s) 289
BseBI CCWGG 1 cut(s) 801
BseDI CCNNGG 7 cut(s) 235, 475, 476, 687, 800, 976, 1113
BseGI GGATG 5 cut(s) 177, 867, 1032, 1123, 1261
BseLI CCNNNNNNNGG 6 cut(s) 74, 330, 346, 688, 745, 1341
BseMII CTCAG 2 cut(s) 444, 828
BseNI ACTGG 1 cut(s) 289
BseRI GAGGAG 1 cut(s) 716
BseSI GKGCMC 1 cut(s) 80
BseXI GCAGC 2 cut(s) 191, 1067
BseYI CCCAGC 1 cut(s) 517
BsgI GTGCAG 1 cut(s) 1083
Bsh1236I CGCG 1 cut(s) 271
BshFI GGCC 4 cut(s) 975, 1112, 1214, 1271
BsiHKAI GWGCWC 2 cut(s) 589, 1190
BsiHKCI CYCGRG 1 cut(s) 475
BsiSI CCGG 4 cut(s) 74, 196, 476, 689
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 6 cut(s) 74, 330, 346, 688, 745, 1341
BsmAI GTCTC 4 cut(s) 344, 384, 410, 1310
BsmBI CGTCTC 3 cut(s) 344, 384, 410
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 4 cut(s) 975, 1112, 1214, 1271
BsoBI CYCGRG 1 cut(s) 475
Bsp1286I GDGCHC 3 cut(s) 80, 589, 1190
Bsp1407I TGTACA 1 cut(s) 792
Bsp143I GATC 3 cut(s) 658, 1075, 1351
BspANI GGCC 4 cut(s) 975, 1112, 1214, 1271
BspCNI CTCAG 2 cut(s) 445, 829
BspFNI CGCG 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 474
BspMAI CTGCAG 2 cut(s) 929, 1282
BspMI ACCTGC 2 cut(s) 98, 866
BspOI GCTAGC 1 cut(s) 990
BspPI GGATC 3 cut(s) 666, 1083, 1346
BspQI GCTCTTC 1 cut(s) 555
BsrBI CCGCTC 1 cut(s) 915
BsrGI TGTACA 1 cut(s) 792
BsrI ACTGG 1 cut(s) 289
BssECI CCNNGG 7 cut(s) 235, 475, 476, 687, 800, 976, 1113
BssMI GATC 3 cut(s) 658, 1075, 1351
BssNI GRCGYC 2 cut(s) 854, 1293
Bst2UI CCWGG 1 cut(s) 801
Bst4CI ACNGT 5 cut(s) 115, 355, 721, 831, 1361
Bst6I CTCTTC 3 cut(s) 555, 673, 1016
BstACI GRCGYC 2 cut(s) 854, 1293
BstAPI GCANNNNNTGC 2 cut(s) 104, 906
BstAUI TGTACA 1 cut(s) 792
BstC8I GCNNGC 3 cut(s) 374, 988, 1392
BstDEI CTNAG 5 cut(s) 453, 596, 837, 1136, 1346
BstDSI CCRYGG 1 cut(s) 1113
BstF5I GGATG 5 cut(s) 177, 867, 1032, 1123, 1261
BstFNI CGCG 1 cut(s) 271
BstH2I RGCGCY 1 cut(s) 205
BstHHI GCGC 2 cut(s) 109, 204
BstKTI GATC 3 cut(s) 661, 1078, 1354
BstMAI GTCTC 4 cut(s) 344, 384, 410, 1310
BstMBI GATC 3 cut(s) 658, 1075, 1351
BstNI CCWGG 1 cut(s) 801
BstNSI RCATGY 1 cut(s) 1244
BstSCI CCNGG 5 cut(s) 73, 474, 475, 687, 799
BstSFI CTRYAG 2 cut(s) 925, 1278
BstSLI GKGCMC 1 cut(s) 80
BstUI CGCG 1 cut(s) 271
BstV1I GCAGC 2 cut(s) 191, 1067
BstX2I RGATCY 1 cut(s) 658
BstXI CCANNNNNNTGG 1 cut(s) 998
BstYI RGATCY 1 cut(s) 658
BsuRI GGCC 4 cut(s) 975, 1112, 1214, 1271
BtgI CCRYGG 1 cut(s) 1113
BtsCI GGATG 5 cut(s) 177, 867, 1032, 1123, 1261
BtsI GCAGTG 3 cut(s) 102, 922, 1275
BtsIMutI CAGTG 3 cut(s) 102, 922, 1275
BveI ACCTGC 2 cut(s) 98, 866
Cac8I GCNNGC 3 cut(s) 374, 988, 1392
CaiI CAGNNNCTG 1 cut(s) 906
CfoI GCGC 2 cut(s) 109, 204
Cfr13I GGNCC 3 cut(s) 333, 472, 1269
Cfr9I CCCGGG 1 cut(s) 475
CseI GACGC 2 cut(s) 843, 1301
Csp6I GTAC 2 cut(s) 793, 1036
CspCI CAANNNNNGTGG 2 cut(s) 1102, 1137
CviAII CATG 4 cut(s) 590, 740, 992, 1241
CviQI GTAC 2 cut(s) 793, 1036
DdeI CTNAG 5 cut(s) 453, 596, 837, 1136, 1346
DpnI GATC 3 cut(s) 660, 1077, 1353
DpnII GATC 3 cut(s) 658, 1075, 1351
EaeI YGGCCR 1 cut(s) 1110
Eam1104I CTCTTC 3 cut(s) 555, 673, 1016
EarI CTCTTC 3 cut(s) 555, 673, 1016
EciI GGCGGA 2 cut(s) 60, 160
Ecl136II GAGCTC 2 cut(s) 587, 1188
Eco147I AGGCCT 1 cut(s) 1214
Eco24I GRGCYC 2 cut(s) 589, 1190
Eco32I GATATC 2 cut(s) 920, 1386
Eco47I GGWCC 2 cut(s) 333, 472
Eco47III AGCGCT 1 cut(s) 203
Eco53kI GAGCTC 2 cut(s) 587, 1188
Eco57I CTGAAG 1 cut(s) 804
Eco88I CYCGRG 1 cut(s) 475
EcoICRI GAGCTC 2 cut(s) 587, 1188
EcoRII CCWGG 1 cut(s) 799
EcoRV GATATC 2 cut(s) 920, 1386
EcoT38I GRGCYC 2 cut(s) 589, 1190
Esp3I CGTCTC 3 cut(s) 344, 384, 410
FaeI CATG 4 cut(s) 593, 743, 995, 1244
FaqI GGGAC 1 cut(s) 493
FatI CATG 4 cut(s) 589, 739, 991, 1240
FauI CCCGC 5 cut(s) 49, 151, 165, 276, 348
FauNDI CATATG 1 cut(s) 1180
FblI GTMKAC 1 cut(s) 606
Fnu4HI GCNGC 6 cut(s) 59, 205, 367, 370, 389, 1081
FokI GGATG 5 cut(s) 164, 874, 1039, 1130, 1268
FriOI GRGCYC 2 cut(s) 589, 1190
Fsp4HI GCNGC 6 cut(s) 59, 205, 367, 370, 389, 1081
FspBI CTAG 2 cut(s) 858, 987
GlaI GCGC 2 cut(s) 108, 203
GluI GCNGC 6 cut(s) 59, 205, 367, 370, 389, 1081
GsaI CCCAGC 1 cut(s) 521
GsuI CTGGAG 1 cut(s) 368
HaeII RGCGCY 1 cut(s) 205
HaeIII GGCC 4 cut(s) 975, 1112, 1214, 1271
HapII CCGG 4 cut(s) 74, 196, 476, 689
HgaI GACGC 2 cut(s) 843, 1301
HhaI GCGC 2 cut(s) 109, 204
Hin1I GRCGYC 2 cut(s) 854, 1293
Hin1II CATG 4 cut(s) 593, 743, 995, 1244
Hin6I GCGC 2 cut(s) 107, 202
HinP1I GCGC 2 cut(s) 107, 202
HindIII AAGCTT 2 cut(s) 13, 573
HpaII CCGG 4 cut(s) 74, 196, 476, 689
HphI GGTGA 2 cut(s) 79, 671
Hpy166II GTNNAC 4 cut(s) 607, 795, 1038, 1306
Hpy188I TCNGA 4 cut(s) 448, 823, 871, 1172
Hpy188III TCNNGA 6 cut(s) 347, 557, 1014, 1146, 1154, 1355
Hpy8I GTNNAC 4 cut(s) 607, 795, 1038, 1306
HpyAV CCTTC 2 cut(s) 828, 1260
HpyCH4III ACNGT 5 cut(s) 115, 355, 721, 831, 1361
HpyCH4IV ACGT 2 cut(s) 285, 419
HpyF3I CTNAG 5 cut(s) 453, 596, 837, 1136, 1346
HpySE526I ACGT 2 cut(s) 285, 419
Hsp92I GRCGYC 2 cut(s) 854, 1293
Hsp92II CATG 4 cut(s) 593, 743, 995, 1244
HspAI GCGC 2 cut(s) 107, 202
Kzo9I GATC 3 cut(s) 658, 1075, 1351
LguI GCTCTTC 1 cut(s) 555
LmnI GCTCC 5 cut(s) 25, 514, 651, 1185, 1414
Lsp1109I GCAGC 2 cut(s) 191, 1067
LweI GCATC 3 cut(s) 478, 852, 862
MaeI CTAG 2 cut(s) 858, 987
MaeII ACGT 2 cut(s) 285, 419
MaeIII GTNAC 3 cut(s) 291, 878, 902
MalI GATC 3 cut(s) 660, 1077, 1353
MbiI CCGCTC 1 cut(s) 915
MboI GATC 3 cut(s) 658, 1075, 1351
MboII GAAGA 7 cut(s) 76, 505, 508, 572, 660, 961, 1033
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 3 cut(s) 80, 589, 1190
MlsI TGGCCA 1 cut(s) 1112
MluCI AATT 8 cut(s) 403, 431, 646, 779, 894, 1054, 1286, 1379
MluNI TGGCCA 1 cut(s) 1112
MmeI TCCRAC 1 cut(s) 758
Mox20I TGGCCA 1 cut(s) 1112
MroXI GAANNNNTTC 1 cut(s) 1166
MscI TGGCCA 1 cut(s) 1112
MseI TTAA 2 cut(s) 6, 402
MslI CAYNNNNRTG 1 cut(s) 996
Msp20I TGGCCA 1 cut(s) 1112
MspA1I CMGCKG 1 cut(s) 836
MspI CCGG 4 cut(s) 74, 196, 476, 689
MspR9I CCNGG 5 cut(s) 75, 476, 477, 689, 801
MvaI CCWGG 1 cut(s) 801
MvnI CGCG 1 cut(s) 271
NciI CCSGG 4 cut(s) 75, 476, 477, 689
NdeI CATATG 1 cut(s) 1180
NdeII GATC 3 cut(s) 658, 1075, 1351
NheI GCTAGC 1 cut(s) 986
NlaIII CATG 4 cut(s) 593, 743, 995, 1244
NlaIV GGNNCC 1 cut(s) 474
NmeAIII GCCGAG 3 cut(s) 216, 326, 957
NspI RCATGY 1 cut(s) 1244
PaqCI CACCTGC 1 cut(s) 98
PceI AGGCCT 1 cut(s) 1214
PciSI GCTCTTC 1 cut(s) 555
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 1166
PkrI GCNGC 6 cut(s) 60, 206, 368, 371, 390, 1082
PsiI TTATAA 1 cut(s) 846
Psp124BI GAGCTC 2 cut(s) 589, 1190
Psp6I CCWGG 1 cut(s) 799
PspFI CCCAGC 1 cut(s) 517
PspGI CCWGG 1 cut(s) 799
PspN4I GGNNCC 1 cut(s) 474
PspPI GGNCC 3 cut(s) 333, 472, 1269
PstI CTGCAG 2 cut(s) 929, 1282
PstNI CAGNNNCTG 1 cut(s) 906
PsuI RGATCY 1 cut(s) 658
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 2 cut(s) 794, 1037
RsaNI GTAC 2 cut(s) 793, 1036
RseI CAYNNNNRTG 1 cut(s) 996
SacI GAGCTC 2 cut(s) 589, 1190
SapI GCTCTTC 1 cut(s) 555
SaqAI TTAA 2 cut(s) 6, 402
SatI GCNGC 6 cut(s) 59, 205, 367, 370, 389, 1081
Sau3AI GATC 3 cut(s) 658, 1075, 1351
Sau96I GGNCC 3 cut(s) 333, 472, 1269
ScrFI CCNGG 5 cut(s) 75, 476, 477, 689, 801
SduI GDGCHC 3 cut(s) 80, 589, 1190
SfaNI GCATC 3 cut(s) 478, 852, 862
SfcI CTRYAG 2 cut(s) 925, 1278
SinI GGWCC 2 cut(s) 333, 472
SmaI CCCGGG 1 cut(s) 477
SmiMI CAYNNNNRTG 1 cut(s) 996
SmlI CTYRAG 1 cut(s) 709
SmoI CTYRAG 1 cut(s) 709
Sse9I AATT 8 cut(s) 403, 431, 646, 779, 894, 1054, 1286, 1379
SseBI AGGCCT 1 cut(s) 1214
SspMI CTAG 2 cut(s) 858, 987
SstI GAGCTC 2 cut(s) 589, 1190
StuI AGGCCT 1 cut(s) 1214
StyD4I CCNGG 5 cut(s) 73, 474, 475, 687, 799
TaaI ACNGT 5 cut(s) 115, 355, 721, 831, 1361
TaiI ACGT 2 cut(s) 288, 422
TaqI TCGA 5 cut(s) 118, 486, 765, 996, 1373
TaqII GACCGA 1 cut(s) 1033
TasI AATT 8 cut(s) 403, 431, 646, 779, 894, 1054, 1286, 1379
TatI WGTACW 2 cut(s) 792, 1035
TauI GCSGC 4 cut(s) 61, 369, 372, 391
Tru1I TTAA 2 cut(s) 6, 402
Tru9I TTAA 2 cut(s) 6, 402
TscAI CASTG 3 cut(s) 109, 929, 1282
TseI GCWGC 2 cut(s) 204, 1080
TspDTI ATGAA 4 cut(s) 1043, 1272, 1299, 1392
TspGWI ACGGA 3 cut(s) 272, 397, 1130
TspMI CCCGGG 1 cut(s) 475
TspRI CASTG 3 cut(s) 109, 929, 1282
VpaK11BI GGWCC 2 cut(s) 333, 472
XapI RAATTY 4 cut(s) 431, 779, 1054, 1286
XceI RCATGY 1 cut(s) 1244
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XmaI CCCGGG 1 cut(s) 475
XmiI GTMKAC 1 cut(s) 606
XmnI GAANNNNTTC 1 cut(s) 1166
XspI CTAG 2 cut(s) 858, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.