MD06G1157200.v1.1

Oxygen-independent coproporphyrinogen-III oxidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009349
Physical Location & Seq
Forward (+)
29928569 .. 29929239
671 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1157200.v1.1.491

Sequence Viewer

Length: 594 bp
ATGACAAAATACGTCCAGTTACTCTGCCGGGAAATCAACGCCACGAAGGCCGAAAACAAGACCGACCCGCCTCTGGAGACGGTGTTTTTTGGCGGCAGCACGCCATCTCTTGTGCCGCCAAAGCTCGTGGATTCCGTTTTGGAGACGTTGAGTCTGAAATTCGGGTTGAGTTCGGAGGCTCAGGTGTCGATGGAGATGGACCAGGGATTGATGGAGTTGGGTGTGAATGGGGTGTCGTTGGGAGTTCAGGCGTTTCAAGAAGAGCTGCTGAAAGCTTGTGGGAGAGCTCATGGCGTTCGGGAGGTTTATGAGGCTACGGAGATTGTTGGGGAATGTGGGGTTGAGAATTGGAGCATGGATCTTATCTCTTCTCTGCCTCACCAGACCCCGGAAATATGGGAGGAGAGCTTGAGGTTGACGGTAGAAGCTCGTCTTACCCATGTGTCAGTTTATGATTTGCAGCCGAACAAGGCACGAAATTCAGGGGGTTGTAAGTATCCACTAGGGGAGTTCCCGTTGCCTTCAGAAATGCGGTCTGCTGAGTTCTATAAGATGGCGTCTAGGACGCCTTCGGATGCAGGTTACAACCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HemN_C Radical_SAM

Protein Analysis

198

Amino Acids

21.69

Weight (kDa)

4.82

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 10 - 139 1.2e-12 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 569
AciI CCGC 4 cut(s) 68, 93, 116, 532
AclWI GGATC 1 cut(s) 366
AcsI RAATTY 2 cut(s) 158, 478
AcuI CTGAAG 1 cut(s) 507
AcyI GRCGYC 2 cut(s) 557, 566
AfiI CCNNNNNNNGG 2 cut(s) 73, 388
AgsI TTSAA 1 cut(s) 257
AhdI GACNNNNNGTC 1 cut(s) 150
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 6 cut(s) 124, 265, 275, 287, 408, 428
AluI AGCT 6 cut(s) 124, 265, 275, 287, 408, 428
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 2 cut(s) 71, 137
AlwI GGATC 1 cut(s) 366
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 3 cut(s) 96, 265, 460
ApoI RAATTY 2 cut(s) 158, 478
AspS9I GGNCC 1 cut(s) 199
AsuC2I CCSGG 2 cut(s) 29, 389
AsuHPI GGTGA 1 cut(s) 371
AvaII GGWCC 1 cut(s) 199
BanII GRGCYC 1 cut(s) 289
BauI CACGAG 1 cut(s) 125
Bbv12I GWGCWC 1 cut(s) 289
BbvI GCAGC 3 cut(s) 108, 252, 472
BccI CCATC 5 cut(s) 112, 184, 190, 205, 547
BciT130I CCWGG 1 cut(s) 203
BciVI GTATCC 1 cut(s) 507
BcnI CCSGG 2 cut(s) 29, 389
BcoDI GTCTC 2 cut(s) 71, 137
BfaI CTAG 3 cut(s) 503, 561, 592
BfuAI ACCTGC 1 cut(s) 569
BfuI GTATCC 1 cut(s) 507
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 5 cut(s) 94, 97, 116, 266, 461
BlsI GCNGC 5 cut(s) 95, 98, 117, 267, 462
Bme1390I CCNGG 3 cut(s) 29, 203, 389
Bme18I GGWCC 1 cut(s) 199
BmeRI GACNNNNNGTC 1 cut(s) 150
BmgT120I GGNCC 1 cut(s) 199
BmrFI CCNGG 3 cut(s) 29, 203, 389
BmsI GCATC 1 cut(s) 565
BpmI CTGGAG 1 cut(s) 95
Bpu10I CCTNAGC 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 430
BpuMI CCSGG 2 cut(s) 29, 389
BsaHI GRCGYC 2 cut(s) 557, 566
BsaJI CCNNGG 2 cut(s) 202, 387
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 500, 530
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 388
Bse1I ACTGG 1 cut(s) 16
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 2 cut(s) 202, 387
BseGI GGATG 1 cut(s) 580
BseLI CCNNNNNNNGG 2 cut(s) 73, 388
BseMII CTCAG 2 cut(s) 194, 531
BseNI ACTGG 1 cut(s) 16
BseRI GAGGAG 1 cut(s) 416
BseXI GCAGC 3 cut(s) 108, 252, 472
BshFI GGCC 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 289
BsiSI CCGG 2 cut(s) 28, 389
BslI CCNNNNNNNGG 2 cut(s) 73, 388
BsmAI GTCTC 2 cut(s) 71, 137
BsmBI CGTCTC 2 cut(s) 71, 137
BsnI GGCC 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 1 cut(s) 358
BspACI CCGC 4 cut(s) 68, 93, 116, 532
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 2 cut(s) 193, 532
BspMI ACCTGC 1 cut(s) 569
BspPI GGATC 1 cut(s) 366
BspQI GCTCTTC 1 cut(s) 255
BsrI ACTGG 1 cut(s) 16
BssECI CCNNGG 2 cut(s) 202, 387
BssMI GATC 1 cut(s) 358
BssNI GRCGYC 2 cut(s) 557, 566
BssSI CACGAG 1 cut(s) 125
Bst2BI CACGAG 1 cut(s) 125
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 2 cut(s) 82, 421
Bst6I CTCTTC 2 cut(s) 255, 373
BstACI GRCGYC 2 cut(s) 557, 566
BstC8I GCNNGC 1 cut(s) 101
BstDEI CTNAG 2 cut(s) 180, 540
BstF5I GGATG 1 cut(s) 580
BstKTI GATC 1 cut(s) 361
BstMAI GTCTC 2 cut(s) 71, 137
BstMBI GATC 1 cut(s) 358
BstMWI GCNNNNNNNGC 2 cut(s) 47, 121
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 3 cut(s) 27, 201, 387
BstV1I GCAGC 3 cut(s) 108, 252, 472
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuI GTATCC 1 cut(s) 507
BsuRI GGCC 1 cut(s) 50
BtsCI GGATG 1 cut(s) 580
BveI ACCTGC 1 cut(s) 569
Cac8I GCNNGC 1 cut(s) 101
Cfr13I GGNCC 1 cut(s) 199
CseI GACGC 2 cut(s) 546, 574
CspCI CAANNNNNGTGG 2 cut(s) 108, 143
CviAII CATG 3 cut(s) 290, 355, 440
DdeI CTNAG 2 cut(s) 180, 540
DpnI GATC 1 cut(s) 360
DpnII GATC 1 cut(s) 358
DriI GACNNNNNGTC 1 cut(s) 150
Eam1104I CTCTTC 2 cut(s) 255, 373
Eam1105I GACNNNNNGTC 1 cut(s) 150
EarI CTCTTC 2 cut(s) 255, 373
Ecl136II GAGCTC 1 cut(s) 287
Eco24I GRGCYC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 287
Eco57I CTGAAG 1 cut(s) 507
EcoICRI GAGCTC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 289
Esp3I CGTCTC 2 cut(s) 71, 137
FaeI CATG 3 cut(s) 293, 358, 443
FaiI YATR 7 cut(s) 291, 309, 356, 397, 441, 453, 549
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FatI CATG 3 cut(s) 289, 354, 439
FauI CCCGC 1 cut(s) 75
Fnu4HI GCNGC 5 cut(s) 94, 97, 116, 266, 461
FokI GGATG 1 cut(s) 587
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 5 cut(s) 94, 97, 116, 266, 461
FspBI CTAG 3 cut(s) 503, 561, 592
GluI GCNGC 5 cut(s) 94, 97, 116, 266, 461
GsuI CTGGAG 1 cut(s) 95
HaeIII GGCC 1 cut(s) 50
HapII CCGG 2 cut(s) 28, 389
HgaI GACGC 2 cut(s) 546, 574
Hin1I GRCGYC 2 cut(s) 557, 566
Hin1II CATG 3 cut(s) 293, 358, 443
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HindIII AAGCTT 1 cut(s) 273
HinfI GANTC 2 cut(s) 131, 151
HpaII CCGG 2 cut(s) 28, 389
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 4 cut(s) 156, 175, 526, 574
Hpy188III TCNNGA 3 cut(s) 74, 257, 299
Hpy8I GTNNAC 1 cut(s) 417
HpyAV CCTTC 3 cut(s) 40, 531, 579
HpyCH4III ACNGT 2 cut(s) 82, 421
HpyCH4IV ACGT 2 cut(s) 12, 146
HpyCH4V TGCA 2 cut(s) 460, 578
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 121
HpyF3I CTNAG 2 cut(s) 180, 540
HpySE526I ACGT 2 cut(s) 12, 146
Hsp92I GRCGYC 2 cut(s) 557, 566
Hsp92II CATG 3 cut(s) 293, 358, 443
Kzo9I GATC 1 cut(s) 358
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 1 cut(s) 351
Lsp1109I GCAGC 3 cut(s) 108, 252, 472
LweI GCATC 1 cut(s) 565
MaeI CTAG 3 cut(s) 503, 561, 592
MaeII ACGT 2 cut(s) 12, 146
MaeIII GTNAC 2 cut(s) 18, 581
MalI GATC 1 cut(s) 360
MboI GATC 1 cut(s) 358
MboII GAAGA 2 cut(s) 272, 360
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 3 cut(s) 158, 346, 478
MlyI GAGTC 1 cut(s) 160
MnlI CCTC 7 cut(s) 81, 169, 295, 304, 387, 394, 405
MspI CCGG 2 cut(s) 28, 389
MspR9I CCNGG 3 cut(s) 29, 203, 389
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 2 cut(s) 47, 121
NciI CCSGG 2 cut(s) 29, 389
NdeII GATC 1 cut(s) 358
NlaIII CATG 3 cut(s) 293, 358, 443
PciSI GCTCTTC 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PfeI GAWTC 1 cut(s) 131
PkrI GCNGC 5 cut(s) 95, 98, 117, 267, 462
PleI GAGTC 1 cut(s) 159
PpsI GAGTC 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspPI GGNCC 1 cut(s) 199
PsuI RGATCY 1 cut(s) 358
SacI GAGCTC 1 cut(s) 289
SapI GCTCTTC 1 cut(s) 255
SatI GCNGC 5 cut(s) 94, 97, 116, 266, 461
Sau3AI GATC 1 cut(s) 358
Sau96I GGNCC 1 cut(s) 199
SchI GAGTC 1 cut(s) 160
ScrFI CCNGG 3 cut(s) 29, 203, 389
SduI GDGCHC 1 cut(s) 289
SfaNI GCATC 1 cut(s) 565
SinI GGWCC 1 cut(s) 199
SmlI CTYRAG 1 cut(s) 409
SmoI CTYRAG 1 cut(s) 409
Sse9I AATT 3 cut(s) 158, 346, 478
SsiI CCGC 4 cut(s) 68, 93, 116, 532
SspMI CTAG 3 cut(s) 503, 561, 592
SstI GAGCTC 1 cut(s) 289
StyD4I CCNGG 3 cut(s) 27, 201, 387
TaaI ACNGT 2 cut(s) 82, 421
TaiI ACGT 2 cut(s) 15, 149
TaqI TCGA 1 cut(s) 188
TaqII GACCGA 1 cut(s) 77
TasI AATT 3 cut(s) 158, 346, 478
TauI GCSGC 2 cut(s) 96, 118
TfiI GAWTC 1 cut(s) 131
TseI GCWGC 3 cut(s) 96, 265, 460
TspGWI ACGGA 2 cut(s) 124, 332
VpaK11BI GGWCC 1 cut(s) 199
XapI RAATTY 2 cut(s) 158, 478
XspI CTAG 3 cut(s) 503, 561, 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.