Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002173

Physical Location & Seq

Genomic Coordinates Forward (+)

26433964 .. 26437222

Gene Structure 3259 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G66130.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1800 bp

ATGCTTAAGAAAAAACTATCCCTAGAAGATGAAGATGACGATAGATCGTATAACTTAAGGTCATCGCGTTCTAATGCCAAGTCGAAACCCAGGTCCTCTGCTGGTACCGCAACGAATCCTAGAGCGTCAAAGAGAGCTCGCCTTTCGGGTGCTAGTGCGACTCAAGAAGACTCGAGTCTTGTAGATAAGATTAGATTGTCTTTCGAAGATTTCGATGAAGCTTTAAGCGGCTTCAAGGTATCTTCTGGTTATGAAAGGAGCAAGAATACCGATTTGTGGGTTGACAAATACAGGCCTCGCACCTTGGAAGAGCTTTCTGTTCACAAGAAGAAGGTTGACGAAGTCAAATTGTGGTTTCAGGAAAGTTTGGATTTCTTAAAGAATGGTTTAAGGAATAACGTTCTACTAGTCACAGGACAAGCTGGAGTCGGAAAATCTGCTACCATCCATTTGATTACGTCTATCCTTGGAGTCACAGTGCATGAGTGGAACGCTCCTATTCCTACCATCTGGCAAGAACATGTGCACAACACTAGTTCAGGACTGAAGTACACGTCGAAGCTGGATGAGTTTGAAAACTTTGTTGAGAGTACTAGGAAATACGGAGTAATGGCTTCATCAAGTACTGACGGAATAAAGGCGCCCGTTGTTCTTTTGATTGATGACCTTCCTCTAGCCAATGGGAGGCATGCTTTTGAAAGGCTGCAAAATTGCCTACTACTATTGGTGAAATCAACGCAGATTCCAACAGTGATATTAATCACTGACCACAATAACGCGGACTCTTCTGACCAGACTGCACGACGCATGGAGGACACACAGTCATCTCTTGAGCGAGCAGGGGCTTTAAAGGTTGCTTTCAATCCCATCACGAAGAACTCAATTAAAAAGGTACTTCAGAGAATATGTAGGGAAGAGCATTGTAAGGTAACAACTATGGAGATTGATCAGATGGCAACTGCAAGTGGAGGCGACATCAGACATGCTATTACATCTTTGCAGCTCTTCTCCGTTAAGCCAGAGCTAAATCATACTAAGATAAAATCACCAAGGCCTGGCATGGAAGACAATTATCATGGAAACGAGCAGACAATGTACAGTGGTTTGGATAGTGGAATATCTTCCTGTTTTGGTAGAGATGAGACCCTTTCGTTGTTTCACGCTCTGGGCAAGTTTCTCCACAACAAAAGAGAGACGGATAATGTCATTATATCAGAGTGCAGCAGCTCTCTTGTTCATGACGAGTTTGCAAGGTTGCCACTGAAAATGGATGCGCCGGAGAAGGTTCTCAGCCAAGCTCATGGACAGGCGGGGCGGGTTGTGGATTTTCTACATGAAAATGTGTTAGATTTTGTGAGTGACGGAGCCATAGAAGATGCCTGGTGTGTTTCTTCGTATTTGGCGGATGCTGATCTCCTTCTCGCTGATATAAGAGGAAAGATGAGTGGACATAACAACACAGAGGATGTTCCACAGTCAGCCGGTGCCTCGGTTGCTGTTCGTGGTGTGTTGTATGGAAACAAGCAGCCATGTTCATCCAGATGGCACGTGATTCGGAAGCCAAAACTCTGGCAAGTGGAGCAATCCTCAATACAAACAAAGAAGAATCTGAGAGAGCAGAGGAACATAAGCTATGAGGGGTCAAGAGTGGCAGATATATCAGTGATGGCCACAGAGTATAGCCCCGTGCTGAAATGGCTTAGCTACAGGGCATCTCCAGATGCGTTTCCGGGAATGGGCGAAGAGACAGATGAGGAGGACAGTGATATTTCTGAAGATGATGAAATACAAGACTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000076 GO:0000077 GO:0000228 GO:0000278 GO:0000785 GO:0000790 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006282 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0019219 GO:0019222 GO:0022402 GO:0031323 GO:0031347 GO:0031570 GO:0031974 GO:0031981 GO:0033314 GO:0033554 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044774 GO:0044818 GO:0045786 GO:0045930 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0070013 GO:0080090 GO:0080134 GO:0080135 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903047 GO:2001020

KEGG Pathways

Metabolic & Signaling

ko04111 ko04113 map04111 map04113

Pfam Domains

Protein Families

Myb_DNA-bind_3 Rad17

Protein Analysis

599

Amino Acids

66.82

Weight (kDa)

5.89

Isoelectric Point (pI)

44.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad17	PF03215	88 - 262	1.2e-24	Rad17 P-loop domain
AAA_lid_RFC1	PF25361	293 - 339	4e-06	RFC1-like, AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002173)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66130 AT5G66130
fragaria_vesca	FvH4_1g11470 FvH4_1g11471
malus_domestica	MD15G1242700.v1.1
prunus_persica	Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171500_v2.0.a1
pyrus_communis	pycom15g21390
rosa_chinensis	RchiOBHm_Chr2g0099051 RchiOBHm_Chr2g0099061
rosa_laevigata	RLG00000016842
rosa_multiflora	Rmu_co8335887.1_g000001 Rmu_sc0000577.1_g000020 Rmu_sc0012098.1_g000008 Rmu_sc0015712.1_g000001 Rmu_sc0025811.1_g000001
rosa_roxburghii	Rroxscaffold_2G00143470
rosa_rugosa	Rorug02G0076000 Rorug02G0076100 Rorug02G0076100 Rorug02G0076200 Rorug02G0076300 Rorug02G0076400
rosa_samantha	Rh2AG125300 Rh2AG125400 Rh2BG128500 Rh2BG128600 Rh2BG128700 Rh2CG129500 Rh2CG129600 Rh2CG129700 Rh2DG129800 Rh2DG129900
rosa_wichuraiana	Rw2G009650 Rw2G009660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	104
AccB1I	GGYRCC	3 cut(s)	104, 640, 1484
AccB7I	CCANNNNNTGG	1 cut(s)	1055
AccII	CGCG	2 cut(s)	67, 779
AciI	CCGC	6 cut(s)	108, 228, 779, 1310, 1315, 1403
AclI	AACGTT	1 cut(s)	399
AcoI	YGGCCR	1 cut(s)	1668
AcuI	CTGAAG	3 cut(s)	566, 881, 1794
AcvI	CACGTG	1 cut(s)	1549
AcyI	GRCGYC	1 cut(s)	641
AfaI	GTAC	6 cut(s)	106, 551, 592, 625, 894, 1097
AfiI	CCNNNNNNNGG	4 cut(s)	276, 684, 1055, 1736
AflII	CTTAAG	2 cut(s)	5, 55
AflIII	ACRYGT	2 cut(s)	520, 552
AgsI	TTSAA	4 cut(s)	235, 575, 698, 862
AhdI	GACNNNNNGTC	1 cut(s)	820
AhlI	ACTAGT	2 cut(s)	406, 533
AjiI	CACGTC	1 cut(s)	555
AjnI	CCWGG	3 cut(s)	89, 1054, 1379
Alw21I	GWGCWC	2 cut(s)	139, 528
Alw26I	GTCTC	3 cut(s)	1136, 1187, 1739
Alw44I	GTGCAC	1 cut(s)	524
Ama87I	CYCGRG	1 cut(s)	172
AoxI	GGCC	3 cut(s)	293, 1052, 1668
ApaLI	GTGCAC	1 cut(s)	524
ApeKI	GCWGC	5 cut(s)	703, 1000, 1221, 1224, 1525
ArsI	GACNNNNNNTTYG	2 cut(s)	77, 109
AseI	ATTAAT	1 cut(s)	758
Asp700I	GAANNNNTTC	1 cut(s)	1120
Asp718I	GGTACC	1 cut(s)	104
AspLEI	GCGC	2 cut(s)	643, 1276
AspS9I	GGNCC	1 cut(s)	93
AsuC2I	CCSGG	1 cut(s)	1731
AsuHPI	GGTGA	2 cut(s)	739, 1038
AsuII	TTCGAA	1 cut(s)	204
AvaI	CYCGRG	1 cut(s)	172
AvaII	GGWCC	1 cut(s)	93
BaeGI	GKGCMC	1 cut(s)	528
BalI	TGGCCA	1 cut(s)	1670
BanI	GGYRCC	3 cut(s)	104, 640, 1484
BanII	GRGCYC	1 cut(s)	139
BbrPI	CACGTG	1 cut(s)	1549
BbsI	GAAGAC	2 cut(s)	174, 1071
Bbv12I	GWGCWC	2 cut(s)	139, 528
BbvI	GCAGC	5 cut(s)	690, 1012, 1233, 1236, 1537
BccI	CCATC	6 cut(s)	452, 515, 875, 946, 1536, 1660
BcgI	CGANNNNNNTGC	2 cut(s)	1535, 1569
BciT130I	CCWGG	3 cut(s)	91, 1056, 1381
BclI	TGATCA	1 cut(s)	946
BcnI	CCSGG	1 cut(s)	1731
BcoDI	GTCTC	3 cut(s)	1136, 1187, 1739
BcuI	ACTAGT	2 cut(s)	406, 533
BfaI	CTAG	7 cut(s)	23, 120, 153, 407, 534, 594, 674
BfmI	CTRYAG	1 cut(s)	1705
BfoI	RGCGCY	1 cut(s)	644
BfrI	CTTAAG	2 cut(s)	5, 55
BisI	GCNGC	6 cut(s)	229, 704, 1001, 1222, 1225, 1526
BlpI	GCTNAGC	1 cut(s)	1700
BlsI	GCNGC	6 cut(s)	230, 705, 1002, 1223, 1226, 1527
BmcAI	AGTACT	2 cut(s)	592, 625
Bme1390I	CCNGG	4 cut(s)	91, 1056, 1381, 1731
Bme18I	GGWCC	1 cut(s)	93
BmeRI	GACNNNNNGTC	1 cut(s)	820
BmeT110I	CYCGRG	1 cut(s)	172
BmgBI	CACGTC	1 cut(s)	555
BmgT120I	GGNCC	1 cut(s)	93
BmiI	GGNNCC	4 cut(s)	106, 642, 1366, 1486
BmrFI	CCNGG	4 cut(s)	91, 1056, 1381, 1731
BmsI	GCATC	5 cut(s)	1261, 1366, 1396, 1711, 1721
BoxI	GACNNNNGTC	1 cut(s)	174
BpiI	GAAGAC	2 cut(s)	174, 1071
BplI	GAGNNNNNCTC	2 cut(s)	1571, 1603
BpmI	CTGGAG	2 cut(s)	444, 1701
Bpu1102I	GCTNAGC	1 cut(s)	1700
Bpu14I	TTCGAA	1 cut(s)	204
BpuEI	CTTGAG	2 cut(s)	147, 851
BpuMI	CCSGG	1 cut(s)	1731
BsaAI	YACGTR	1 cut(s)	1549
BsaBI	GATNNNNATC	2 cut(s)	758, 1410
BsaHI	GRCGYC	1 cut(s)	641
BsaI	GGTCTC	1 cut(s)	1136
BsaJI	CCNNGG	5 cut(s)	89, 303, 466, 1049, 1488
BsaXI	ACNNNNNCTCC	2 cut(s)	462, 492
Bsc4I	CCNNNNNNNGG	4 cut(s)	276, 684, 1055, 1736
Bse118I	RCCGGY	1 cut(s)	1481
Bse1I	ACTGG	1 cut(s)	1799
Bse8I	GATNNNNATC	2 cut(s)	758, 1410
BseBI	CCWGG	3 cut(s)	91, 1056, 1381
BseDI	CCNNGG	5 cut(s)	89, 303, 466, 1049, 1488
BseGI	GGATG	6 cut(s)	444, 571, 1276, 1411, 1471, 1535
BseJI	GATNNNNATC	2 cut(s)	758, 1410
BseLI	CCNNNNNNNGG	4 cut(s)	276, 684, 1055, 1736
BseMII	CTCAG	2 cut(s)	1303, 1601
BseNI	ACTGG	1 cut(s)	1799
BseRI	GAGGAG	1 cut(s)	1769
BseSI	GKGCMC	1 cut(s)	528
BseXI	GCAGC	5 cut(s)	690, 1012, 1233, 1236, 1537
BsgI	GTGCAG	2 cut(s)	783, 1240
Bsh1236I	CGCG	2 cut(s)	67, 779
BshFI	GGCC	3 cut(s)	295, 1054, 1670
BshNI	GGYRCC	3 cut(s)	104, 640, 1484
BsiHKAI	GWGCWC	2 cut(s)	139, 528
BsiHKCI	CYCGRG	1 cut(s)	172
BsiSI	CCGG	3 cut(s)	1277, 1482, 1730
BslI	CCNNNNNNNGG	4 cut(s)	276, 684, 1055, 1736
BsmAI	GTCTC	3 cut(s)	1136, 1187, 1739
BsmBI	CGTCTC	1 cut(s)	1187
BsnI	GGCC	3 cut(s)	295, 1054, 1670
Bso31I	GGTCTC	1 cut(s)	1136
BsoBI	CYCGRG	1 cut(s)	172
Bsp119I	TTCGAA	1 cut(s)	204
Bsp1286I	GDGCHC	2 cut(s)	139, 528
Bsp1407I	TGTACA	1 cut(s)	1095
Bsp143I	GATC	3 cut(s)	44, 946, 1411
Bsp1720I	GCTNAGC	1 cut(s)	1700
BspACI	CCGC	6 cut(s)	108, 228, 779, 1310, 1315, 1403
BspANI	GGCC	3 cut(s)	295, 1054, 1670
BspCNI	CTCAG	2 cut(s)	1302, 1602
BspFNI	CGCG	2 cut(s)	67, 779
BspHI	TCATGA	1 cut(s)	1237
BspLI	GGNNCC	4 cut(s)	106, 642, 1366, 1486
BspQI	GCTCTTC	3 cut(s)	303, 909, 1010
BspT104I	TTCGAA	1 cut(s)	204
BspT107I	GGYRCC	3 cut(s)	104, 640, 1484
BspTI	CTTAAG	2 cut(s)	5, 55
BspTNI	GGTCTC	1 cut(s)	1136
BsrFI	RCCGGY	1 cut(s)	1481
BsrGI	TGTACA	1 cut(s)	1095
BsrI	ACTGG	1 cut(s)	1799
BssAI	RCCGGY	1 cut(s)	1481
BssECI	CCNNGG	5 cut(s)	89, 303, 466, 1049, 1488
BssMI	GATC	3 cut(s)	44, 946, 1411
BssNI	GRCGYC	1 cut(s)	641
BssT1I	CCWWGG	3 cut(s)	303, 466, 1049
Bst2UI	CCWGG	3 cut(s)	91, 1056, 1381
Bst4CI	ACNGT	6 cut(s)	478, 751, 822, 1100, 1476, 1763
Bst6I	CTCTTC	5 cut(s)	303, 790, 909, 1010, 1737
BstACI	GRCGYC	1 cut(s)	641
BstAFI	CTTAAG	2 cut(s)	5, 55
BstAUI	TGTACA	1 cut(s)	1095
BstBAI	YACGTR	1 cut(s)	1549
BstBI	TTCGAA	1 cut(s)	204
BstC8I	GCNNGC	3 cut(s)	139, 690, 837
BstDEI	CTNAG	4 cut(s)	1035, 1289, 1610, 1700
BstF5I	GGATG	6 cut(s)	444, 571, 1276, 1411, 1471, 1535
BstFNI	CGCG	2 cut(s)	67, 779
BstH2I	RGCGCY	1 cut(s)	644
BstHHI	GCGC	2 cut(s)	643, 1276
BstKTI	GATC	3 cut(s)	47, 949, 1414
BstMAI	GTCTC	3 cut(s)	1136, 1187, 1739
BstMBI	GATC	3 cut(s)	44, 946, 1411
BstMWI	GCNNNNNNNGC	4 cut(s)	107, 1493, 1579, 1696
BstNI	CCWGG	3 cut(s)	91, 1056, 1381
BstNSI	RCATGY	3 cut(s)	524, 692, 986
BstPAI	GACNNNNGTC	1 cut(s)	174
BstSCI	CCNGG	4 cut(s)	89, 1054, 1379, 1729
BstSFI	CTRYAG	1 cut(s)	1705
BstSLI	GKGCMC	1 cut(s)	528
BstUI	CGCG	2 cut(s)	67, 779
BstV1I	GCAGC	5 cut(s)	690, 1012, 1233, 1236, 1537
BstV2I	GAAGAC	2 cut(s)	174, 1071
BstXI	CCANNNNNNTGG	2 cut(s)	1301, 1569
BsuRI	GGCC	3 cut(s)	295, 1054, 1670
BtgZI	GCGATG	1 cut(s)	48
BtrI	CACGTC	1 cut(s)	555
BtsCI	GGATG	6 cut(s)	444, 571, 1276, 1411, 1471, 1535
BtsIMutI	CAGTG	7 cut(s)	483, 756, 762, 1105, 1259, 1668, 1768
Cac8I	GCNNGC	3 cut(s)	139, 690, 837
CciI	TCATGA	1 cut(s)	1237
CfoI	GCGC	2 cut(s)	643, 1276
Cfr10I	RCCGGY	1 cut(s)	1481
Cfr13I	GGNCC	1 cut(s)	93
CseI	GACGC	2 cut(s)	114, 813
Csp6I	GTAC	6 cut(s)	105, 550, 591, 624, 893, 1096
CviQI	GTAC	6 cut(s)	105, 550, 591, 624, 893, 1096
DdeI	CTNAG	4 cut(s)	1035, 1289, 1610, 1700
DinI	GGCGCC	1 cut(s)	642
DpnI	GATC	3 cut(s)	46, 948, 1413
DpnII	GATC	3 cut(s)	44, 946, 1411
DraI	TTTAAA	1 cut(s)	849
DriI	GACNNNNNGTC	1 cut(s)	820
EaeI	YGGCCR	1 cut(s)	1668
Eam1104I	CTCTTC	5 cut(s)	303, 790, 909, 1010, 1737
Eam1105I	GACNNNNNGTC	1 cut(s)	820
EarI	CTCTTC	5 cut(s)	303, 790, 909, 1010, 1737
EciI	GGCGGA	1 cut(s)	1418
Ecl136II	GAGCTC	1 cut(s)	137
Eco130I	CCWWGG	3 cut(s)	303, 466, 1049
Eco147I	AGGCCT	2 cut(s)	295, 1054
Eco24I	GRGCYC	1 cut(s)	139
Eco31I	GGTCTC	1 cut(s)	1136
Eco47I	GGWCC	1 cut(s)	93
Eco53kI	GAGCTC	1 cut(s)	137
Eco57I	CTGAAG	3 cut(s)	566, 881, 1794
Eco72I	CACGTG	1 cut(s)	1549
Eco88I	CYCGRG	1 cut(s)	172
EcoICRI	GAGCTC	1 cut(s)	137
EcoO109I	RGGNCCY	1 cut(s)	93
EcoRII	CCWGG	3 cut(s)	89, 1054, 1379
EcoT14I	CCWWGG	3 cut(s)	303, 466, 1049
EcoT38I	GRGCYC	1 cut(s)	139
EgeI	GGCGCC	1 cut(s)	642
EheI	GGCGCC	1 cut(s)	642
ErhI	CCWWGG	3 cut(s)	303, 466, 1049
Esp3I	CGTCTC	1 cut(s)	1187
FauI	CCCGC	2 cut(s)	1303, 1308
FbaI	TGATCA	1 cut(s)	946
Fnu4HI	GCNGC	6 cut(s)	229, 704, 1001, 1222, 1225, 1526
FokI	GGATG	6 cut(s)	431, 578, 1283, 1418, 1478, 1522
FriOI	GRGCYC	1 cut(s)	139
Fsp4HI	GCNGC	6 cut(s)	229, 704, 1001, 1222, 1225, 1526
FspBI	CTAG	7 cut(s)	23, 120, 153, 407, 534, 594, 674
GlaI	GCGC	2 cut(s)	642, 1275
GluI	GCNGC	6 cut(s)	229, 704, 1001, 1222, 1225, 1526
GsuI	CTGGAG	2 cut(s)	444, 1701
HaeII	RGCGCY	1 cut(s)	644
HaeIII	GGCC	3 cut(s)	295, 1054, 1670
HapII	CCGG	3 cut(s)	1277, 1482, 1730
HgaI	GACGC	2 cut(s)	114, 813
HhaI	GCGC	2 cut(s)	643, 1276
Hin1I	GRCGYC	1 cut(s)	641
Hin6I	GCGC	2 cut(s)	641, 1274
HinP1I	GCGC	2 cut(s)	641, 1274
HincII	GTYRAC	2 cut(s)	283, 337
HindII	GTYRAC	2 cut(s)	283, 337
HindIII	AAGCTT	1 cut(s)	219
HpaII	CCGG	3 cut(s)	1277, 1482, 1730
HphI	GGTGA	2 cut(s)	739, 1038
Hpy166II	GTNNAC	6 cut(s)	283, 322, 337, 526, 552, 1448
Hpy188I	TCNGA	9 cut(s)	431, 790, 900, 951, 980, 1216, 1557, 1611, 1774
Hpy188III	TCNNGA	9 cut(s)	164, 359, 540, 830, 871, 1238, 1539, 1644, 1718
Hpy8I	GTNNAC	6 cut(s)	283, 322, 337, 526, 552, 1448
Hpy99I	CGWCG	2 cut(s)	559, 807
HpyAV	CCTTC	4 cut(s)	325, 677, 1276, 1427
HpyCH4III	ACNGT	6 cut(s)	478, 751, 822, 1100, 1476, 1763
HpyCH4IV	ACGT	4 cut(s)	399, 458, 554, 1548
HpyCH4V	TGCA	8 cut(s)	481, 526, 706, 800, 962, 1000, 1221, 1250
HpyF10VI	GCNNNNNNNGC	4 cut(s)	107, 1493, 1579, 1696
HpyF3I	CTNAG	4 cut(s)	1035, 1289, 1610, 1700
HpySE526I	ACGT	4 cut(s)	399, 458, 554, 1548
Hsp92I	GRCGYC	1 cut(s)	641
HspAI	GCGC	2 cut(s)	641, 1274
KasI	GGCGCC	1 cut(s)	640
KpnI	GGTACC	1 cut(s)	108
Ksp22I	TGATCA	1 cut(s)	946
Kzo9I	GATC	3 cut(s)	44, 946, 1411
LguI	GCTCTTC	3 cut(s)	303, 909, 1010
LmnI	GCTCC	4 cut(s)	258, 499, 1364, 1579
Lsp1109I	GCAGC	5 cut(s)	690, 1012, 1233, 1236, 1537
LweI	GCATC	5 cut(s)	1261, 1366, 1396, 1711, 1721
MaeI	CTAG	7 cut(s)	23, 120, 153, 407, 534, 594, 674
MaeII	ACGT	4 cut(s)	399, 458, 554, 1548
MaeIII	GTNAC	4 cut(s)	409, 472, 928, 1358
MalI	GATC	3 cut(s)	46, 948, 1413
MboI	GATC	3 cut(s)	44, 946, 1411
MhlI	GDGCHC	2 cut(s)	139, 528
MlsI	TGGCCA	1 cut(s)	1670
MluCI	AATT	4 cut(s)	347, 709, 882, 1069
MluNI	TGGCCA	1 cut(s)	1670
Mly113I	GGCGCC	1 cut(s)	641
MlyI	GAGTC	6 cut(s)	154, 164, 184, 435, 480, 776
MmeI	TCCRAC	2 cut(s)	409, 770
Mox20I	TGGCCA	1 cut(s)	1670
MroXI	GAANNNNTTC	1 cut(s)	1120
MscI	TGGCCA	1 cut(s)	1670
MseI	TTAA	9 cut(s)	6, 56, 224, 377, 389, 758, 848, 885, 1014
MslI	CAYNNNNRTG	2 cut(s)	1338, 1540
Msp20I	TGGCCA	1 cut(s)	1670
MspCI	CTTAAG	2 cut(s)	5, 55
MspI	CCGG	3 cut(s)	1277, 1482, 1730
MspR9I	CCNGG	4 cut(s)	91, 1056, 1381, 1731
MvaI	CCWGG	3 cut(s)	91, 1056, 1381
MvnI	CGCG	2 cut(s)	67, 779
MwoI	GCNNNNNNNGC	4 cut(s)	107, 1493, 1579, 1696
NarI	GGCGCC	1 cut(s)	641
NciI	CCSGG	1 cut(s)	1731
NdeII	GATC	3 cut(s)	44, 946, 1411
NlaIV	GGNNCC	4 cut(s)	106, 642, 1366, 1486
NmuCI	GTSAC	3 cut(s)	409, 472, 1358
NspI	RCATGY	3 cut(s)	524, 692, 986
NspV	TTCGAA	1 cut(s)	204
PaeI	GCATGC	1 cut(s)	692
PaeR7I	CTCGAG	1 cut(s)	172
PagI	TCATGA	1 cut(s)	1237
PceI	AGGCCT	2 cut(s)	295, 1054
PciI	ACATGT	1 cut(s)	520
PciSI	GCTCTTC	3 cut(s)	303, 909, 1010
PcsI	WCGNNNNNNNCGW	1 cut(s)	210
PdmI	GAANNNNTTC	1 cut(s)	1120
PfeI	GAWTC	4 cut(s)	115, 742, 1552, 1606
PflFI	GACNNNGTC	1 cut(s)	341
PflMI	CCANNNNNTGG	1 cut(s)	1055
PfoI	TCCNGGA	1 cut(s)	1729
PkrI	GCNGC	6 cut(s)	230, 705, 1002, 1223, 1226, 1527
PleI	GAGTC	6 cut(s)	154, 164, 183, 434, 479, 776
PluTI	GGCGCC	1 cut(s)	644
PmaCI	CACGTG	1 cut(s)	1549
PmlI	CACGTG	1 cut(s)	1549
PpsI	GAGTC	6 cut(s)	154, 164, 183, 434, 479, 776
Ppu21I	YACGTR	1 cut(s)	1549
PpuMI	RGGWCCY	1 cut(s)	93
PscI	ACATGT	1 cut(s)	520
PshAI	GACNNNNGTC	1 cut(s)	174
PshBI	ATTAAT	1 cut(s)	758
Psp124BI	GAGCTC	1 cut(s)	139
Psp1406I	AACGTT	1 cut(s)	399
Psp5II	RGGWCCY	1 cut(s)	93
Psp6I	CCWGG	3 cut(s)	89, 1054, 1379
PspCI	CACGTG	1 cut(s)	1549
PspGI	CCWGG	3 cut(s)	89, 1054, 1379
PspN4I	GGNNCC	4 cut(s)	106, 642, 1366, 1486
PspPI	GGNCC	1 cut(s)	93
PspPPI	RGGWCCY	1 cut(s)	93
PspXI	VCTCGAGB	1 cut(s)	172
PsyI	GACNNNGTC	1 cut(s)	341
RsaI	GTAC	6 cut(s)	106, 551, 592, 625, 894, 1097
RsaNI	GTAC	6 cut(s)	105, 550, 591, 624, 893, 1096
RseI	CAYNNNNRTG	2 cut(s)	1338, 1540
SacI	GAGCTC	1 cut(s)	139
SapI	GCTCTTC	3 cut(s)	303, 909, 1010
SaqAI	TTAA	9 cut(s)	6, 56, 224, 377, 389, 758, 848, 885, 1014
SatI	GCNGC	6 cut(s)	229, 704, 1001, 1222, 1225, 1526
Sau3AI	GATC	3 cut(s)	44, 946, 1411
Sau96I	GGNCC	1 cut(s)	93
ScaI	AGTACT	2 cut(s)	592, 625
SchI	GAGTC	6 cut(s)	154, 164, 184, 435, 480, 776
ScrFI	CCNGG	4 cut(s)	91, 1056, 1381, 1731
SduI	GDGCHC	2 cut(s)	139, 528
SfaNI	GCATC	5 cut(s)	1261, 1366, 1396, 1711, 1721
SfcI	CTRYAG	1 cut(s)	1705
SfoI	GGCGCC	1 cut(s)	642
Sfr274I	CTCGAG	1 cut(s)	172
SfuI	TTCGAA	1 cut(s)	204
SinI	GGWCC	1 cut(s)	93
SlaI	CTCGAG	1 cut(s)	172
SmiMI	CAYNNNNRTG	2 cut(s)	1338, 1540
SmlI	CTYRAG	5 cut(s)	5, 55, 162, 172, 830
SmoI	CTYRAG	5 cut(s)	5, 55, 162, 172, 830
SpeI	ACTAGT	2 cut(s)	406, 533
SphI	GCATGC	1 cut(s)	692
Sse9I	AATT	4 cut(s)	347, 709, 882, 1069
SseBI	AGGCCT	2 cut(s)	295, 1054
SsiI	CCGC	6 cut(s)	108, 228, 779, 1310, 1315, 1403
SspDI	GGCGCC	1 cut(s)	640
SspMI	CTAG	7 cut(s)	23, 120, 153, 407, 534, 594, 674
SstI	GAGCTC	1 cut(s)	139
StuI	AGGCCT	2 cut(s)	295, 1054
StyD4I	CCNGG	4 cut(s)	89, 1054, 1379, 1729
StyI	CCWWGG	3 cut(s)	303, 466, 1049
TaaI	ACNGT	6 cut(s)	478, 751, 822, 1100, 1476, 1763
TaiI	ACGT	4 cut(s)	402, 461, 557, 1551
TaqI	TCGA	5 cut(s)	83, 173, 204, 213, 557
TasI	AATT	4 cut(s)	347, 709, 882, 1069
TatI	WGTACW	4 cut(s)	549, 590, 623, 1095
TauI	GCSGC	1 cut(s)	231
TfiI	GAWTC	4 cut(s)	115, 742, 1552, 1606
Tru1I	TTAA	9 cut(s)	6, 56, 224, 377, 389, 758, 848, 885, 1014
Tru9I	TTAA	9 cut(s)	6, 56, 224, 377, 389, 758, 848, 885, 1014
TscAI	CASTG	7 cut(s)	483, 756, 769, 1105, 1266, 1668, 1768
TseFI	GTSAC	3 cut(s)	409, 472, 1358
TseI	GCWGC	5 cut(s)	703, 1000, 1221, 1224, 1525
Tsp45I	GTSAC	3 cut(s)	409, 472, 1358
TspDTI	ATGAA	8 cut(s)	45, 231, 267, 606, 1226, 1350, 1524, 1797
TspGWI	ACGGA	5 cut(s)	618, 645, 1000, 1211, 1377
TspRI	CASTG	7 cut(s)	483, 756, 769, 1105, 1266, 1668, 1768
Tth111I	GACNNNGTC	1 cut(s)	341
Van91I	CCANNNNNTGG	1 cut(s)	1055
Vha464I	CTTAAG	2 cut(s)	5, 55
VneI	GTGCAC	1 cut(s)	524
VpaK11BI	GGWCC	1 cut(s)	93
VspI	ATTAAT	1 cut(s)	758
XceI	RCATGY	3 cut(s)	524, 692, 986
XhoI	CTCGAG	1 cut(s)	172
XmnI	GAANNNNTTC	1 cut(s)	1120
XspI	CTAG	7 cut(s)	23, 120, 153, 407, 534, 594, 674
ZrmI	AGTACT	2 cut(s)	592, 625

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.