Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002173

Physical Location & Seq

Genomic Coordinates Forward (+)

19957966 .. 19962912

Gene Structure 4947 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1242700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1797 bp

ATGGGGAAGAGGAACACTGTGGTGGTGTTGTCATCGGACAACGAAGACGTCGATCGTTCATCGAGCGCGAATCGCAGAAAAACGCAATCGAAATCGAGCTCTTCGGTTCCGCGAACAAACCCTAGGCGATCCAAAAGGGCTCGGGTTTCGGGTTCTCGCTCTAGCTTGGGCACGGCACCGAGCAATTGGGATGAGGTTAAATTGTTTTGTGAAGATTTTGACGATGGGTTCAAGGTCTCTGCTGCTTCTGGAAGAAGCAACGTGAAGGAGTTATGGGTGGATAAATACAAACCCCGTTCTTTTGTGGACCTTGCTGTTCACAAGAAAAAGGTCGAAGAAGTTAAGGTGTGGTTTGAAGAAAGATTGAGAGATTCAAAGGGTGCATCTAGGAATCATGTTCTTGTCATCACTGGGCAAGCTGGTGTTGGAAAATCTGCAACTGTTCACGTGATTGCATCTCACCTTGGAGCCACATTGTGTGAATGGAACACGCCAACTCCAATAGTTTGGGAAGAACATTTATACAACTCCAGTGCAGGAATACGCTACATGTCAAAGTTGGATGAATTTGAAAATTTTGTGGAGAGAATAAGGAAGTATGGATTGATCCCATCATCCCTTAATGAGGGTTCAAAATCTTCGTTTATACTCTTAATTGATGACCTTCCGGTGACAAATGGTAAAGCTGCCTTTGGAAGACTTCAAAACTGCTTGCATCTTCTTGTGAAATCTACTCAGATACCAACTGCTATATTGGTCACTGACTATGGTAAAGCTGATTCAGCTGATCACACCACTGGATACTTAGAAGAAATTCCATCGTTTCTTGAAAGTGTTGGGGCTTGTAAGGTCTCCTTCAATCCTATAACAGATAATTCCGTTAAGAAAGTACTTTCTAGAATATGCAGAGAAGAGCAGTGTACTGTGACTGCTGAACAGGTCAATCTAATAGCAAAAGCAAGTGGAGGTGACATCCGACATGCAATTACATCATTACAATTCTTCTTTCTGAAACCAACTCCGAGGAATTTGTTGTCTTCATTGAGTCCTGCGCCCAGGCATGCTAAGGAGAAGCCAGATGAGGTAAATGCTTTGGACAGCGGGTTTTCCTTCCAGTTTGGCAGAGATGATACACTTTCTCTATTCCATGCACTTGGGAAGTTTCTGCATAATAAAAGAGAGACTGAAAATGTCATGACATTGGACGGAGATGCATTTCATGTGCGGGAAGGATTGTCTAGACTACCACTAAAAATGGATGCTCCAGAAAAGATTCTTTGTCAAGCACACGGACAAGCTAGACCTATTGCCGATTTTCTTCATGAAAATGTTCTAGATTTCCTAAGCGAGGAGGCAATAGATGATGCATGGACAATTGCTTCATACTTAAGTGATGCTGACTTGCTTCTTGCTACTTTCCATGGAATGCGAAGTAGACAGTATGAGGCAGATAATGTTTTACAGTCATCTGGTGCTTCAGTTGCTGTCCGTGGTGTGCTCTTTGGAAATTTTCATCCATCGCCTTCCAGGTGGCATGCCGTTCGCCGGCCAAAGCTCTGGCAGGTTGAGCAATCATCAATGCTTAACAAGAAGGAGATGGTGAAACGGAGATTGGATGCATATAATGGAATGTGCGGTGCCTCCGATTTGACAGTTATAGCTACCGAGTACACACCTGTGTTGAAATGGCTTGGATTTAGGGCAGGGCAAGGGTACAAGGAAGAAGGCGATACCGAAAAGATGAGTATGGATGATGAAGAGAGCGAAATTTCGGATGATGACATAGAAGAATGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000076 GO:0000077 GO:0000228 GO:0000278 GO:0000785 GO:0000790 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006282 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0019219 GO:0019222 GO:0022402 GO:0031323 GO:0031347 GO:0031570 GO:0031974 GO:0031981 GO:0033314 GO:0033554 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044774 GO:0044818 GO:0045786 GO:0045930 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0070013 GO:0080090 GO:0080134 GO:0080135 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903047 GO:2001020

KEGG Pathways

Metabolic & Signaling

ko04111 ko04113 map04111 map04113

Pfam Domains

Protein Families

Myb_DNA-bind_3 Rad17

Protein Analysis

599

Amino Acids

66.73

Weight (kDa)

6.41

Isoelectric Point (pI)

45.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad17	PF03215	89 - 255	1.3e-21	Rad17 P-loop domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002173)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66130 AT5G66130
fragaria_vesca	FvH4_1g11470 FvH4_1g11471
malus_domestica	MD15G1242700.v1.1
prunus_persica	Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171400_v2.0.a1 Prupe.7G171500_v2.0.a1
pyrus_communis	pycom15g21390
rosa_chinensis	RchiOBHm_Chr2g0099051 RchiOBHm_Chr2g0099061
rosa_laevigata	RLG00000016842
rosa_multiflora	Rmu_co8335887.1_g000001 Rmu_sc0000577.1_g000020 Rmu_sc0012098.1_g000008 Rmu_sc0015712.1_g000001 Rmu_sc0025811.1_g000001
rosa_roxburghii	Rroxscaffold_2G00143470
rosa_rugosa	Rorug02G0076000 Rorug02G0076100 Rorug02G0076100 Rorug02G0076200 Rorug02G0076300 Rorug02G0076400
rosa_samantha	Rh2AG125300 Rh2AG125400 Rh2BG128500 Rh2BG128600 Rh2BG128700 Rh2CG129500 Rh2CG129600 Rh2CG129700 Rh2DG129800 Rh2DG129900
rosa_wichuraiana	Rw2G009650 Rw2G009660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	51
Acc36I	ACCTGC	1 cut(s)	1552
AccB1I	GGYRCC	2 cut(s)	175, 1637
AccI	GTMKAC	1 cut(s)	1435
AccII	CGCG	2 cut(s)	68, 112
AciI	CCGC	4 cut(s)	110, 1101, 1225, 1635
AclWI	GGATC	2 cut(s)	123, 601
AcoI	YGGCCR	1 cut(s)	1547
AcsI	RAATTY	6 cut(s)	566, 574, 813, 1027, 1507, 1767
AcuI	CTGAAG	1 cut(s)	1461
AcvI	CACGTG	1 cut(s)	448
AcyI	GRCGYC	1 cut(s)	48
AdeI	CACNNNGTG	1 cut(s)	477
AfaI	GTAC	4 cut(s)	891, 922, 1670, 1715
AfiI	CCNNNNNNNGG	3 cut(s)	625, 1348, 1545
AflII	CTTAAG	1 cut(s)	1387
AflIII	ACRYGT	1 cut(s)	549
AgsI	TTSAA	9 cut(s)	232, 356, 375, 572, 633, 704, 830, 859, 1684
AjnI	CCWGG	2 cut(s)	1055, 1526
AjuI	GAANNNNNNNTTGG	2 cut(s)	1595, 1627
AleI	CACNNNNGTG	2 cut(s)	20, 1676
AluBI	AGCT	9 cut(s)	99, 165, 419, 686, 776, 785, 1298, 1555, 1661
AluI	AGCT	9 cut(s)	99, 165, 419, 686, 776, 785, 1298, 1555, 1661
Alw21I	GWGCWC	2 cut(s)	101, 1500
Alw26I	GTCTC	3 cut(s)	241, 856, 1175
AlwI	GGATC	2 cut(s)	123, 601
AlwNI	CAGNNNCTG	1 cut(s)	1484
Ama87I	CYCGRG	1 cut(s)	141
AoxI	GGCC	1 cut(s)	1547
ApeKI	GCWGC	2 cut(s)	242, 686
ApoI	RAATTY	6 cut(s)	566, 574, 813, 1027, 1507, 1767
Asp700I	GAANNNNTTC	3 cut(s)	813, 1272, 1329
AspA2I	CCTAGG	1 cut(s)	122
AspLEI	GCGC	2 cut(s)	68, 1054
AspS9I	GGNCC	1 cut(s)	307
AsuHPI	GGTGA	4 cut(s)	452, 682, 980, 1612
AvaI	CYCGRG	1 cut(s)	141
AvaII	GGWCC	1 cut(s)	307
AvrII	CCTAGG	1 cut(s)	122
BaeGI	GKGCMC	1 cut(s)	173
BanI	GGYRCC	2 cut(s)	175, 1637
BanII	GRGCYC	2 cut(s)	101, 142
BbrPI	CACGTG	1 cut(s)	448
BbsI	GAAGAC	3 cut(s)	51, 703, 1029
Bbv12I	GWGCWC	2 cut(s)	101, 1500
BbvI	GCAGC	2 cut(s)	229, 673
BccI	CCATC	5 cut(s)	218, 619, 826, 1525, 1591
BceAI	ACGGC	2 cut(s)	189, 1523
BcgI	CGANNNNNNTGC	2 cut(s)	1523, 1557
BciT130I	CCWGG	2 cut(s)	1057, 1528
BciVI	GTATCC	1 cut(s)	794
BclI	TGATCA	1 cut(s)	787
BcoDI	GTCTC	3 cut(s)	241, 856, 1175
BfaI	CTAG	7 cut(s)	123, 162, 387, 897, 1239, 1299, 1334
BfrI	CTTAAG	1 cut(s)	1387
BfuAI	ACCTGC	1 cut(s)	1552
BfuI	GTATCC	1 cut(s)	794
BisI	GCNGC	2 cut(s)	243, 687
BlnI	CCTAGG	1 cut(s)	122
BlsI	GCNGC	2 cut(s)	244, 688
BmcAI	AGTACT	1 cut(s)	891
Bme1390I	CCNGG	2 cut(s)	1057, 1528
Bme18I	GGWCC	1 cut(s)	307
BmeT110I	CYCGRG	1 cut(s)	141
BmgT120I	GGNCC	1 cut(s)	307
BmiI	GGNNCC	4 cut(s)	108, 177, 469, 1639
BmrFI	CCNGG	2 cut(s)	1057, 1528
BmrI	ACTGGG	1 cut(s)	420
BmsI	GCATC	8 cut(s)	392, 464, 724, 1201, 1249, 1354, 1384, 1606
BmuI	ACTGGG	1 cut(s)	420
BpiI	GAAGAC	3 cut(s)	51, 703, 1029
BpmI	CTGGAG	2 cut(s)	514, 1248
Bpu10I	CCTNAGC	2 cut(s)	1065, 1343
BsaAI	YACGTR	1 cut(s)	448
BsaHI	GRCGYC	1 cut(s)	48
BsaI	GGTCTC	2 cut(s)	241, 856
BsaJI	CCNNGG	6 cut(s)	122, 463, 1022, 1055, 1420, 1489
BsaWI	WCCGGW	1 cut(s)	667
BsaXI	ACNNNNNCTCC	2 cut(s)	481, 511
Bsc4I	CCNNNNNNNGG	3 cut(s)	625, 1348, 1545
Bse118I	RCCGGY	1 cut(s)	1545
Bse1I	ACTGG	4 cut(s)	415, 531, 802, 1114
BseBI	CCWGG	2 cut(s)	1057, 1528
BseDI	CCNNGG	6 cut(s)	122, 463, 1022, 1055, 1420, 1489
BseGI	GGATG	9 cut(s)	196, 568, 614, 972, 1264, 1513, 1621, 1756, 1780
BseLI	CCNNNNNNNGG	3 cut(s)	625, 1348, 1545
BseMII	CTCAG	1 cut(s)	749
BseNI	ACTGG	4 cut(s)	415, 531, 802, 1114
BseRI	GAGGAG	1 cut(s)	1364
BseSI	GKGCMC	1 cut(s)	173
BseXI	GCAGC	2 cut(s)	229, 673
BsgI	GTGCAG	1 cut(s)	555
Bsh1236I	CGCG	2 cut(s)	68, 112
Bsh1285I	CGRYCG	1 cut(s)	55
BshFI	GGCC	1 cut(s)	1549
BshNI	GGYRCC	2 cut(s)	175, 1637
BsiEI	CGRYCG	1 cut(s)	55
BsiHKAI	GWGCWC	2 cut(s)	101, 1500
BsiHKCI	CYCGRG	1 cut(s)	141
BsiSI	CCGG	2 cut(s)	668, 1546
BslI	CCNNNNNNNGG	3 cut(s)	625, 1348, 1545
BsmAI	GTCTC	3 cut(s)	241, 856, 1175
BsmI	GAATGC	1 cut(s)	1431
BsnI	GGCC	1 cut(s)	1549
Bso31I	GGTCTC	2 cut(s)	241, 856
BsoBI	CYCGRG	1 cut(s)	141
Bsp1286I	GDGCHC	4 cut(s)	101, 142, 173, 1500
Bsp143I	GATC	4 cut(s)	52, 128, 606, 787
Bsp19I	CCATGG	1 cut(s)	1420
BspACI	CCGC	4 cut(s)	110, 1101, 1225, 1635
BspANI	GGCC	1 cut(s)	1549
BspCNI	CTCAG	1 cut(s)	748
BspFNI	CGCG	2 cut(s)	68, 112
BspHI	TCATGA	2 cut(s)	1194, 1321
BspLI	GGNNCC	4 cut(s)	108, 177, 469, 1639
BspMI	ACCTGC	1 cut(s)	1552
BspPI	GGATC	2 cut(s)	123, 601
BspQI	GCTCTTC	2 cut(s)	106, 906
BspT107I	GGYRCC	2 cut(s)	175, 1637
BspTI	CTTAAG	1 cut(s)	1387
BspTNI	GGTCTC	2 cut(s)	241, 856
BsrFI	RCCGGY	1 cut(s)	1545
BsrI	ACTGG	4 cut(s)	415, 531, 802, 1114
BssAI	RCCGGY	1 cut(s)	1545
BssECI	CCNNGG	6 cut(s)	122, 463, 1022, 1055, 1420, 1489
BssMI	GATC	4 cut(s)	52, 128, 606, 787
BssNI	GRCGYC	1 cut(s)	48
BssT1I	CCWWGG	3 cut(s)	122, 463, 1420
Bst2UI	CCWGG	2 cut(s)	1057, 1528
Bst4CI	ACNGT	6 cut(s)	19, 442, 925, 1440, 1464, 1654
Bst6I	CTCTTC	4 cut(s)	2, 106, 906, 1752
BstACI	GRCGYC	1 cut(s)	48
BstAFI	CTTAAG	1 cut(s)	1387
BstBAI	YACGTR	1 cut(s)	448
BstC8I	GCNNGC	5 cut(s)	417, 713, 1062, 1536, 1547
BstDEI	CTNAG	4 cut(s)	735, 805, 1065, 1343
BstDSI	CCRYGG	2 cut(s)	1420, 1489
BstENI	CCTNNNNNAGG	2 cut(s)	623, 1346
BstF5I	GGATG	9 cut(s)	196, 568, 614, 972, 1264, 1513, 1621, 1756, 1780
BstFNI	CGCG	2 cut(s)	68, 112
BstHHI	GCGC	2 cut(s)	68, 1054
BstKTI	GATC	4 cut(s)	55, 131, 609, 790
BstMAI	GTCTC	3 cut(s)	241, 856, 1175
BstMBI	GATC	4 cut(s)	52, 128, 606, 787
BstMCI	CGRYCG	1 cut(s)	55
BstMWI	GCNNNNNNNGC	4 cut(s)	72, 782, 1481, 1567
BstNI	CCWGG	2 cut(s)	1057, 1528
BstNSI	RCATGY	4 cut(s)	553, 983, 1064, 1538
BstSCI	CCNGG	2 cut(s)	1055, 1526
BstSLI	GKGCMC	1 cut(s)	173
BstUI	CGCG	2 cut(s)	68, 112
BstV1I	GCAGC	2 cut(s)	229, 673
BstV2I	GAAGAC	3 cut(s)	51, 703, 1029
BstXI	CCANNNNNNTGG	3 cut(s)	507, 1154, 1557
BsuI	GTATCC	1 cut(s)	794
BsuRI	GGCC	1 cut(s)	1549
BtgI	CCRYGG	2 cut(s)	1420, 1489
BtgZI	GCGATG	1 cut(s)	1503
BtsCI	GGATG	9 cut(s)	196, 568, 614, 972, 1264, 1513, 1621, 1756, 1780
BtsI	GCAGTG	1 cut(s)	923
BtsIMutI	CAGTG	6 cut(s)	15, 408, 538, 759, 795, 923
BveI	ACCTGC	1 cut(s)	1552
Cac8I	GCNNGC	5 cut(s)	417, 713, 1062, 1536, 1547
CaiI	CAGNNNCTG	1 cut(s)	1484
CciI	TCATGA	2 cut(s)	1194, 1321
CfoI	GCGC	2 cut(s)	68, 1054
Cfr10I	RCCGGY	1 cut(s)	1545
Cfr13I	GGNCC	1 cut(s)	307
Csp6I	GTAC	4 cut(s)	890, 921, 1669, 1714
CviQI	GTAC	4 cut(s)	890, 921, 1669, 1714
DdeI	CTNAG	4 cut(s)	735, 805, 1065, 1343
DpnI	GATC	4 cut(s)	54, 130, 608, 789
DpnII	GATC	4 cut(s)	52, 128, 606, 787
DraIII	CACNNNGTG	1 cut(s)	477
EaeI	YGGCCR	1 cut(s)	1547
Eam1104I	CTCTTC	4 cut(s)	2, 106, 906, 1752
EarI	CTCTTC	4 cut(s)	2, 106, 906, 1752
Ecl136II	GAGCTC	1 cut(s)	99
Eco130I	CCWWGG	3 cut(s)	122, 463, 1420
Eco24I	GRGCYC	2 cut(s)	101, 142
Eco31I	GGTCTC	2 cut(s)	241, 856
Eco47I	GGWCC	1 cut(s)	307
Eco53kI	GAGCTC	1 cut(s)	99
Eco57I	CTGAAG	1 cut(s)	1461
Eco72I	CACGTG	1 cut(s)	448
Eco88I	CYCGRG	1 cut(s)	141
EcoICRI	GAGCTC	1 cut(s)	99
EcoNI	CCTNNNNNAGG	2 cut(s)	623, 1346
EcoRII	CCWGG	2 cut(s)	1055, 1526
EcoT14I	CCWWGG	3 cut(s)	122, 463, 1420
EcoT22I	ATGCAT	3 cut(s)	1216, 1369, 1621
EcoT38I	GRGCYC	2 cut(s)	101, 142
ErhI	CCWWGG	3 cut(s)	122, 463, 1420
FalI	AAGNNNNNCTT	2 cut(s)	839, 871
FauI	CCCGC	2 cut(s)	1094, 1218
FbaI	TGATCA	1 cut(s)	787
FblI	GTMKAC	1 cut(s)	1435
Fnu4HI	GCNGC	2 cut(s)	243, 687
FokI	GGATG	9 cut(s)	203, 575, 601, 959, 1271, 1500, 1628, 1763, 1787
FriOI	GRGCYC	2 cut(s)	101, 142
Fsp4HI	GCNGC	2 cut(s)	243, 687
FspBI	CTAG	7 cut(s)	123, 162, 387, 897, 1239, 1299, 1334
GlaI	GCGC	2 cut(s)	67, 1053
GluI	GCNGC	2 cut(s)	243, 687
GsuI	CTGGAG	2 cut(s)	514, 1248
HaeIII	GGCC	1 cut(s)	1549
HapII	CCGG	2 cut(s)	668, 1546
HhaI	GCGC	2 cut(s)	68, 1054
Hin1I	GRCGYC	1 cut(s)	48
Hin6I	GCGC	2 cut(s)	66, 1052
HinP1I	GCGC	2 cut(s)	66, 1052
HinfI	GANTC	6 cut(s)	70, 371, 391, 779, 1045, 1273
HpaII	CCGG	2 cut(s)	668, 1546
HphI	GGTGA	4 cut(s)	452, 682, 980, 1612
Hpy166II	GTNNAC	6 cut(s)	307, 319, 445, 921, 1436, 1671
Hpy188I	TCNGA	7 cut(s)	37, 738, 977, 1011, 1023, 1645, 1774
Hpy188III	TCNNGA	8 cut(s)	249, 827, 897, 1195, 1239, 1265, 1322, 1334
Hpy8I	GTNNAC	6 cut(s)	307, 319, 445, 921, 1436, 1671
Hpy99I	CGWCG	1 cut(s)	53
HpyAV	CCTTC	8 cut(s)	259, 674, 865, 1120, 1223, 1533, 1585, 1718
HpyCH4III	ACNGT	6 cut(s)	19, 442, 925, 1440, 1464, 1654
HpyCH4IV	ACGT	3 cut(s)	48, 261, 447
HpyF10VI	GCNNNNNNNGC	4 cut(s)	72, 782, 1481, 1567
HpyF3I	CTNAG	4 cut(s)	735, 805, 1065, 1343
HpySE526I	ACGT	3 cut(s)	48, 261, 447
Hsp92I	GRCGYC	1 cut(s)	48
HspAI	GCGC	2 cut(s)	66, 1052
KroI	GCCGGC	1 cut(s)	1545
KroNI	GCCGGC	1 cut(s)	1547
Ksp22I	TGATCA	1 cut(s)	787
Kzo9I	GATC	4 cut(s)	52, 128, 606, 787
LguI	GCTCTTC	2 cut(s)	106, 906
LmnI	GCTCC	2 cut(s)	467, 1267
Lsp1109I	GCAGC	2 cut(s)	229, 673
LweI	GCATC	8 cut(s)	392, 464, 724, 1201, 1249, 1354, 1384, 1606
MaeI	CTAG	7 cut(s)	123, 162, 387, 897, 1239, 1299, 1334
MaeII	ACGT	3 cut(s)	48, 261, 447
MaeIII	GTNAC	4 cut(s)	670, 757, 925, 968
MalI	GATC	4 cut(s)	54, 130, 608, 789
MboI	GATC	4 cut(s)	52, 128, 606, 787
MfeI	CAATTG	2 cut(s)	184, 1374
MhlI	GDGCHC	4 cut(s)	101, 142, 173, 1500
MlyI	GAGTC	1 cut(s)	1054
MmeI	TCCRAC	3 cut(s)	406, 540, 1000
Mph1103I	ATGCAT	3 cut(s)	1216, 1369, 1621
MroNI	GCCGGC	1 cut(s)	1545
MroXI	GAANNNNTTC	3 cut(s)	813, 1272, 1329
MseI	TTAA	7 cut(s)	198, 342, 621, 653, 882, 1388, 1584
MslI	CAYNNNNRTG	3 cut(s)	20, 1326, 1676
MspA1I	CMGCKG	2 cut(s)	785, 1101
MspCI	CTTAAG	1 cut(s)	1387
MspI	CCGG	2 cut(s)	668, 1546
MspR9I	CCNGG	2 cut(s)	1057, 1528
MunI	CAATTG	2 cut(s)	184, 1374
Mva1269I	GAATGC	1 cut(s)	1431
MvaI	CCWGG	2 cut(s)	1057, 1528
MvnI	CGCG	2 cut(s)	68, 112
MwoI	GCNNNNNNNGC	4 cut(s)	72, 782, 1481, 1567
NaeI	GCCGGC	1 cut(s)	1547
NcoI	CCATGG	1 cut(s)	1420
NdeII	GATC	4 cut(s)	52, 128, 606, 787
NgoMIV	GCCGGC	1 cut(s)	1545
NlaIV	GGNNCC	4 cut(s)	108, 177, 469, 1639
NmuCI	GTSAC	4 cut(s)	670, 757, 925, 968
NsiI	ATGCAT	3 cut(s)	1216, 1369, 1621
NspI	RCATGY	4 cut(s)	553, 983, 1064, 1538
OliI	CACNNNNGTG	2 cut(s)	20, 1676
PaeI	GCATGC	2 cut(s)	1064, 1538
PagI	TCATGA	2 cut(s)	1194, 1321
PciI	ACATGT	1 cut(s)	549
PciSI	GCTCTTC	2 cut(s)	106, 906
PcsI	WCGNNNNNNNCGW	1 cut(s)	48
PctI	GAATGC	1 cut(s)	1431
PdiI	GCCGGC	1 cut(s)	1547
PdmI	GAANNNNTTC	3 cut(s)	813, 1272, 1329
PfeI	GAWTC	5 cut(s)	70, 371, 391, 779, 1273
PkrI	GCNGC	2 cut(s)	244, 688
Ple19I	CGATCG	1 cut(s)	55
PleI	GAGTC	1 cut(s)	1053
PmaCI	CACGTG	1 cut(s)	448
PmlI	CACGTG	1 cut(s)	448
PpsI	GAGTC	1 cut(s)	1053
Ppu21I	YACGTR	1 cut(s)	448
PscI	ACATGT	1 cut(s)	549
Psp124BI	GAGCTC	1 cut(s)	101
Psp6I	CCWGG	2 cut(s)	1055, 1526
PspCI	CACGTG	1 cut(s)	448
PspGI	CCWGG	2 cut(s)	1055, 1526
PspN4I	GGNNCC	4 cut(s)	108, 177, 469, 1639
PspPI	GGNCC	1 cut(s)	307
PstNI	CAGNNNCTG	1 cut(s)	1484
PvuI	CGATCG	1 cut(s)	55
PvuII	CAGCTG	1 cut(s)	785
RsaI	GTAC	4 cut(s)	891, 922, 1670, 1715
RsaNI	GTAC	4 cut(s)	890, 921, 1669, 1714
RseI	CAYNNNNRTG	3 cut(s)	20, 1326, 1676
SacI	GAGCTC	1 cut(s)	101
SapI	GCTCTTC	2 cut(s)	106, 906
SaqAI	TTAA	7 cut(s)	198, 342, 621, 653, 882, 1388, 1584
SatI	GCNGC	2 cut(s)	243, 687
Sau3AI	GATC	4 cut(s)	52, 128, 606, 787
Sau96I	GGNCC	1 cut(s)	307
ScaI	AGTACT	1 cut(s)	891
SchI	GAGTC	1 cut(s)	1054
ScrFI	CCNGG	2 cut(s)	1057, 1528
SduI	GDGCHC	4 cut(s)	101, 142, 173, 1500
SfaNI	GCATC	8 cut(s)	392, 464, 724, 1201, 1249, 1354, 1384, 1606
SinI	GGWCC	1 cut(s)	307
SmiMI	CAYNNNNRTG	3 cut(s)	20, 1326, 1676
SmlI	CTYRAG	1 cut(s)	1387
SmoI	CTYRAG	1 cut(s)	1387
SphI	GCATGC	2 cut(s)	1064, 1538
SsiI	CCGC	4 cut(s)	110, 1101, 1225, 1635
SspMI	CTAG	7 cut(s)	123, 162, 387, 897, 1239, 1299, 1334
SstI	GAGCTC	1 cut(s)	101
StyD4I	CCNGG	2 cut(s)	1055, 1526
StyI	CCWWGG	3 cut(s)	122, 463, 1420
TaaI	ACNGT	6 cut(s)	19, 442, 925, 1440, 1464, 1654
TaiI	ACGT	3 cut(s)	51, 264, 450
TaqI	TCGA	5 cut(s)	51, 62, 89, 95, 333
TatI	WGTACW	3 cut(s)	889, 920, 1668
TfiI	GAWTC	5 cut(s)	70, 371, 391, 779, 1273
Tru1I	TTAA	7 cut(s)	198, 342, 621, 653, 882, 1388, 1584
Tru9I	TTAA	7 cut(s)	198, 342, 621, 653, 882, 1388, 1584
TscAI	CASTG	6 cut(s)	22, 415, 538, 766, 802, 923
TseFI	GTSAC	4 cut(s)	670, 757, 925, 968
TseI	GCWGC	2 cut(s)	242, 686
Tsp45I	GTSAC	4 cut(s)	670, 757, 925, 968
TspDTI	ATGAA	9 cut(s)	48, 579, 1029, 1208, 1310, 1338, 1371, 1502, 1770
TspGWI	ACGGA	5 cut(s)	868, 1221, 1305, 1478, 1621
TspRI	CASTG	6 cut(s)	22, 415, 538, 766, 802, 923
Vha464I	CTTAAG	1 cut(s)	1387
VpaK11BI	GGWCC	1 cut(s)	307
XagI	CCTNNNNNAGG	2 cut(s)	623, 1346
XapI	RAATTY	6 cut(s)	566, 574, 813, 1027, 1507, 1767
XbaI	TCTAGA	3 cut(s)	896, 1238, 1333
XceI	RCATGY	4 cut(s)	553, 983, 1064, 1538
XmaJI	CCTAGG	1 cut(s)	122
XmiI	GTMKAC	1 cut(s)	1435
XmnI	GAANNNNTTC	3 cut(s)	813, 1272, 1329
XspI	CTAG	7 cut(s)	123, 162, 387, 897, 1239, 1299, 1334
ZraI	GACGTC	1 cut(s)	49
ZrmI	AGTACT	1 cut(s)	891
Zsp2I	ATGCAT	3 cut(s)	1216, 1369, 1621

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.