AT5G66240

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005287
Physical Location & Seq
Forward (+)
26466172 .. 26468484
2313 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66240.3

Sequence Viewer

Length: 987 bp
ATGGTTGAGAGAGAGGACCGAGTTTCTTTGGAACTCAGCGAAGAAATTATACAGAGCATGGAACCTGGCGCCGTTTTCAGAGACTATAATTGCAGGATCAGCTCAATTGACTTTCACAAAACTTCGACTTGTATGGTGACAGCTAGCGACGATGACTCAATTCGTCTCTATGATGTTGCCAGCGCCACATGTCTGAAGACAATTAATAGCAAAAAGTATGGTGTTGATCTTGTTTGCTTCACTTCTCATCCTACTACTGTCATCTACTCTTCCAGAAACGGATGGGACGACTCTCTGAGGCTTCTCTCTTTGCACGATAATAAGTACTTGCGCTACTTCAAAGGACATCATGACAGAGTTGTTTCGCTTAGCTTGTGTTCTGGTGGGGAATGCTTCATCTCCGGTTCCCTTGATCGTACTGTTTTGCTTTGGGATCAAAGGGTCGAGAAATGCCAGGGTCTCTTACGAGTCCAAGGAAGGCCTGCTGCAGCATATGATGATCAAGGGTTAATTTTTGCAATTGCCTTTGGTGGCTACATAAGAATGTTTGATGCCCGTATGTACGAAAAGGGTCCTTTTGAAATATTTTCCGTTGGTGGTGATCTTTCTGAGGCAAACGTTGTGAAATTTAGTAATGATGGAAGGCTCATGCTGCTTACAACCATGGATGGTTTTATTCATGTGCTTGACTCGTTTCGTGGAACTCTTCTGTCCACATTTAGTGTGAAACCAGTAGCTGGAGAATCTACATTGGATGCAGCCTTTAGTCCTGAAGGGATGTTTGTTGTTTCAGGTTCAGGTGATGGTAGTACCCATGCATGGGGTGTAAGGAGCGGAAAACAGGTACATAGTTGGATGGGTTTAGGGAGTGAGCCCCCAGTCATTAAATGGGGTCCAGGAAGTCCCATGTTTGTGACAGGCTCTTCCGAACTTGCTTTTGTTATACCCGACTTATCGAAATTGCCAGCTTATGCCATTCGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
WD40

Protein Analysis

328

Amino Acids

36.14

Weight (kDa)

5.77

Isoelectric Point (pI)

36.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 23 - 223 7.9e-19 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 26 - 104 2.2e-07 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 30 - 150 5e-11 CDC20/Fizzy WD40 domain
WDR55 PF24796 31 - 151 1.4e-06 WDR55
Beta-prop_WDR5 PF25175 32 - 307 8.4e-32 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 32 - 92 9.3e-06 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 33 - 149 4.6e-10 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 40 - 160 3.6e-06 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 41 - 240 8e-14 WDHD1 first WD40 domain
NBCH_WD40 PF20426 94 - 147 1.1e-06 Neurobeachin beta propeller domain
Beta-prop_THOC3 PF25174 96 - 292 2.2e-13 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 96 - 159 2e-06 WDR3 first beta-propeller domain
WD40 PF00400 108 - 145 1.5e-06 WD domain, G-beta repeat
WD40_Gbeta PF25391 112 - 234 1.8e-06 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 129 - 282 1.8e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 68
AccB7I CCANNNNNTGG 1 cut(s) 737
AccBSI CCGCTC 1 cut(s) 834
AciI CCGC 1 cut(s) 834
AclI AACGTT 1 cut(s) 618
AclWI GGATC 2 cut(s) 104, 441
AcsI RAATTY 1 cut(s) 626
AcuI CTGAAG 2 cut(s) 215, 792
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 5 cut(s) 326, 418, 563, 811, 846
AfiI CCNNNNNNNGG 3 cut(s) 737, 819, 820
AflIII ACRYGT 1 cut(s) 188
AgsI TTSAA 2 cut(s) 340, 581
AjnI CCWGG 3 cut(s) 64, 453, 895
AluBI AGCT 5 cut(s) 102, 143, 372, 737, 968
AluI AGCT 5 cut(s) 102, 143, 372, 737, 968
Alw26I GTCTC 3 cut(s) 75, 170, 464
AlwI GGATC 2 cut(s) 104, 441
AlwNI CAGNNNCTG 1 cut(s) 737
AoxI GGCC 1 cut(s) 479
ApeKI GCWGC 4 cut(s) 485, 488, 652, 758
ApoI RAATTY 1 cut(s) 626
AseI ATTAAT 1 cut(s) 204
AspLEI GCGC 3 cut(s) 71, 185, 333
AspS9I GGNCC 3 cut(s) 16, 572, 893
AsuHPI GGTGA 3 cut(s) 148, 611, 812
AsuNHI GCTAGC 1 cut(s) 143
AvaII GGWCC 3 cut(s) 16, 572, 893
BanI GGYRCC 1 cut(s) 68
BanII GRGCYC 1 cut(s) 876
BarI GAAGNNNNNNTAC 2 cut(s) 33, 65
BbsI GAAGAC 1 cut(s) 203
BbvI GCAGC 4 cut(s) 472, 500, 639, 770
BccI CCATC 5 cut(s) 276, 632, 662, 797, 850
BceAI ACGGC 1 cut(s) 56
BciT130I CCWGG 3 cut(s) 66, 455, 897
BclI TGATCA 1 cut(s) 499
BcoDI GTCTC 3 cut(s) 75, 170, 464
BfaI CTAG 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 486
BfoI RGCGCY 2 cut(s) 72, 186
BisI GCNGC 4 cut(s) 486, 489, 653, 759
BlpI GCTNAGC 1 cut(s) 368
BlsI GCNGC 4 cut(s) 487, 490, 654, 760
BmcAI AGTACT 1 cut(s) 326
Bme1390I CCNGG 3 cut(s) 66, 455, 897
Bme18I GGWCC 3 cut(s) 16, 572, 893
BmgT120I GGNCC 3 cut(s) 16, 572, 893
BmiI GGNNCC 5 cut(s) 63, 70, 406, 573, 894
BmrFI CCNGG 3 cut(s) 66, 455, 897
BmrI ACTGGG 1 cut(s) 872
BmsI GCATC 2 cut(s) 541, 745
BmtI GCTAGC 1 cut(s) 147
BmuI ACTGGG 1 cut(s) 872
BpiI GAAGAC 1 cut(s) 203
BpmI CTGGAG 1 cut(s) 759
Bpu1102I GCTNAGC 1 cut(s) 368
BsaHI GRCGYC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 464
BsaJI CCNNGG 3 cut(s) 454, 472, 663
BsaWI WCCGGW 1 cut(s) 401
Bsc4I CCNNNNNNNGG 3 cut(s) 737, 819, 820
Bse1I ACTGG 2 cut(s) 731, 878
BseBI CCWGG 3 cut(s) 66, 455, 897
BseDI CCNNGG 3 cut(s) 454, 472, 663
BseGI GGATG 6 cut(s) 247, 287, 673, 760, 783, 861
BseLI CCNNNNNNNGG 3 cut(s) 737, 819, 820
BseMII CTCAG 3 cut(s) 49, 287, 600
BseNI ACTGG 2 cut(s) 731, 878
BseXI GCAGC 4 cut(s) 472, 500, 639, 770
BshFI GGCC 1 cut(s) 481
BshNI GGYRCC 1 cut(s) 68
BsiSI CCGG 1 cut(s) 402
BslFI GGGAC 2 cut(s) 299, 888
BslI CCNNNNNNNGG 3 cut(s) 737, 819, 820
BsmAI GTCTC 3 cut(s) 75, 170, 464
BsmBI CGTCTC 1 cut(s) 170
BsmFI GGGAC 2 cut(s) 299, 888
BsmI GAATGC 1 cut(s) 395
BsnI GGCC 1 cut(s) 481
Bso31I GGTCTC 1 cut(s) 464
Bsp1286I GDGCHC 1 cut(s) 876
Bsp143I GATC 6 cut(s) 96, 226, 412, 433, 499, 601
Bsp1720I GCTNAGC 1 cut(s) 368
Bsp19I CCATGG 1 cut(s) 663
BspACI CCGC 1 cut(s) 834
BspANI GGCC 1 cut(s) 481
BspCNI CTCAG 3 cut(s) 48, 288, 601
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 5 cut(s) 63, 70, 406, 573, 894
BspMAI CTGCAG 1 cut(s) 490
BspOI GCTAGC 1 cut(s) 147
BspPI GGATC 2 cut(s) 104, 441
BspQI GCTCTTC 1 cut(s) 928
BspT107I GGYRCC 1 cut(s) 68
BspTNI GGTCTC 1 cut(s) 464
BsrBI CCGCTC 1 cut(s) 834
BsrI ACTGG 2 cut(s) 731, 878
BssECI CCNNGG 3 cut(s) 454, 472, 663
BssMI GATC 6 cut(s) 96, 226, 412, 433, 499, 601
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 2 cut(s) 472, 663
Bst2UI CCWGG 3 cut(s) 66, 455, 897
Bst4CI ACNGT 2 cut(s) 259, 421
Bst6I CTCTTC 3 cut(s) 274, 711, 928
BstACI GRCGYC 1 cut(s) 69
BstC8I GCNNGC 4 cut(s) 145, 181, 483, 966
BstDEI CTNAG 4 cut(s) 35, 296, 368, 609
BstDSI CCRYGG 1 cut(s) 663
BstF5I GGATG 6 cut(s) 247, 287, 673, 760, 783, 861
BstH2I RGCGCY 2 cut(s) 72, 186
BstHHI GCGC 3 cut(s) 71, 185, 333
BstKTI GATC 6 cut(s) 99, 229, 415, 436, 502, 604
BstMAI GTCTC 3 cut(s) 75, 170, 464
BstMBI GATC 6 cut(s) 96, 226, 412, 433, 499, 601
BstMWI GCNNNNNNNGC 2 cut(s) 99, 652
BstNI CCWGG 3 cut(s) 66, 455, 897
BstNSI RCATGY 1 cut(s) 192
BstSCI CCNGG 3 cut(s) 64, 453, 895
BstSFI CTRYAG 1 cut(s) 486
BstV1I GCAGC 4 cut(s) 472, 500, 639, 770
BstV2I GAAGAC 1 cut(s) 203
BsuRI GGCC 1 cut(s) 481
BtgI CCRYGG 1 cut(s) 663
BtsCI GGATG 6 cut(s) 247, 287, 673, 760, 783, 861
Cac8I GCNNGC 4 cut(s) 145, 181, 483, 966
CaiI CAGNNNCTG 1 cut(s) 737
CciI TCATGA 1 cut(s) 349
CfoI GCGC 3 cut(s) 71, 185, 333
Cfr13I GGNCC 3 cut(s) 16, 572, 893
Csp6I GTAC 5 cut(s) 325, 417, 562, 810, 845
CviAII CATG 9 cut(s) 58, 189, 350, 649, 664, 680, 815, 819, 907
CviQI GTAC 5 cut(s) 325, 417, 562, 810, 845
DdeI CTNAG 4 cut(s) 35, 296, 368, 609
DinI GGCGCC 1 cut(s) 70
DpnI GATC 6 cut(s) 98, 228, 414, 435, 501, 603
DpnII GATC 6 cut(s) 96, 226, 412, 433, 499, 601
Eam1104I CTCTTC 3 cut(s) 274, 711, 928
EarI CTCTTC 3 cut(s) 274, 711, 928
Eco130I CCWWGG 2 cut(s) 472, 663
Eco147I AGGCCT 1 cut(s) 481
Eco24I GRGCYC 1 cut(s) 876
Eco31I GGTCTC 1 cut(s) 464
Eco47I GGWCC 3 cut(s) 16, 572, 893
Eco57I CTGAAG 2 cut(s) 215, 792
EcoO109I RGGNCCY 1 cut(s) 572
EcoRII CCWGG 3 cut(s) 64, 453, 895
EcoT14I CCWWGG 2 cut(s) 472, 663
EcoT22I ATGCAT 1 cut(s) 820
EcoT38I GRGCYC 1 cut(s) 876
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
ErhI CCWWGG 2 cut(s) 472, 663
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 9 cut(s) 61, 192, 353, 652, 667, 683, 818, 822, 910
FaqI GGGAC 2 cut(s) 299, 888
FatI CATG 9 cut(s) 57, 188, 349, 648, 663, 679, 814, 818, 906
FauNDI CATATG 1 cut(s) 493
FbaI TGATCA 1 cut(s) 499
Fnu4HI GCNGC 4 cut(s) 486, 489, 653, 759
FokI GGATG 6 cut(s) 234, 294, 680, 767, 790, 868
FriOI GRGCYC 1 cut(s) 876
Fsp4HI GCNGC 4 cut(s) 486, 489, 653, 759
FspBI CTAG 1 cut(s) 144
GlaI GCGC 3 cut(s) 70, 184, 332
GluI GCNGC 4 cut(s) 486, 489, 653, 759
GsuI CTGGAG 1 cut(s) 759
HaeII RGCGCY 2 cut(s) 72, 186
HaeIII GGCC 1 cut(s) 481
HapII CCGG 1 cut(s) 402
HhaI GCGC 3 cut(s) 71, 185, 333
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 9 cut(s) 61, 192, 353, 652, 667, 683, 818, 822, 910
Hin6I GCGC 3 cut(s) 69, 183, 331
HinP1I GCGC 3 cut(s) 69, 183, 331
HinfI GANTC 5 cut(s) 155, 290, 468, 689, 743
HpaII CCGG 1 cut(s) 402
HphI GGTGA 3 cut(s) 148, 611, 812
Hpy166II GTNNAC 1 cut(s) 714
Hpy188I TCNGA 6 cut(s) 80, 195, 297, 610, 928, 981
Hpy188III TCNNGA 4 cut(s) 273, 350, 445, 770
Hpy8I GTNNAC 1 cut(s) 714
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 3 cut(s) 471, 636, 767
HpyCH4III ACNGT 2 cut(s) 259, 421
HpyCH4IV ACGT 1 cut(s) 618
HpyCH4V TGCA 6 cut(s) 93, 313, 488, 518, 758, 818
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 652
HpyF3I CTNAG 4 cut(s) 35, 296, 368, 609
HpySE526I ACGT 1 cut(s) 618
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 9 cut(s) 61, 192, 353, 652, 667, 683, 818, 822, 910
HspAI GCGC 3 cut(s) 69, 183, 331
KasI GGCGCC 1 cut(s) 68
Ksp22I TGATCA 1 cut(s) 499
Kzo9I GATC 6 cut(s) 96, 226, 412, 433, 499, 601
LguI GCTCTTC 1 cut(s) 928
LmnI GCTCC 1 cut(s) 831
Lsp1109I GCAGC 4 cut(s) 472, 500, 639, 770
LweI GCATC 2 cut(s) 541, 745
MaeI CTAG 1 cut(s) 144
MaeII ACGT 1 cut(s) 618
MaeIII GTNAC 2 cut(s) 136, 913
MalI GATC 6 cut(s) 98, 228, 414, 435, 501, 603
MbiI CCGCTC 1 cut(s) 834
MboI GATC 6 cut(s) 96, 226, 412, 433, 499, 601
MboII GAAGA 5 cut(s) 53, 208, 261, 698, 915
MfeI CAATTG 2 cut(s) 105, 519
MhlI GDGCHC 1 cut(s) 876
MluCI AATT 9 cut(s) 45, 88, 105, 159, 201, 510, 519, 626, 959
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 4 cut(s) 149, 284, 477, 683
MmeI TCCRAC 1 cut(s) 833
MnlI CCTC 3 cut(s) 7, 291, 604
Mph1103I ATGCAT 1 cut(s) 820
MseI TTAA 3 cut(s) 204, 509, 885
MslI CAYNNNNRTG 2 cut(s) 542, 911
MspI CCGG 1 cut(s) 402
MspR9I CCNGG 3 cut(s) 66, 455, 897
MunI CAATTG 2 cut(s) 105, 519
Mva1269I GAATGC 1 cut(s) 395
MvaI CCWGG 3 cut(s) 66, 455, 897
MwoI GCNNNNNNNGC 2 cut(s) 99, 652
NarI GGCGCC 1 cut(s) 69
NcoI CCATGG 1 cut(s) 663
NdeI CATATG 1 cut(s) 493
NdeII GATC 6 cut(s) 96, 226, 412, 433, 499, 601
NheI GCTAGC 1 cut(s) 143
NlaIII CATG 9 cut(s) 61, 192, 353, 652, 667, 683, 818, 822, 910
NlaIV GGNNCC 5 cut(s) 63, 70, 406, 573, 894
NmuCI GTSAC 2 cut(s) 136, 913
NsiI ATGCAT 1 cut(s) 820
NspI RCATGY 1 cut(s) 192
PagI TCATGA 1 cut(s) 349
PceI AGGCCT 1 cut(s) 481
PciI ACATGT 1 cut(s) 188
PciSI GCTCTTC 1 cut(s) 928
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 1 cut(s) 395
PfeI GAWTC 1 cut(s) 743
PflMI CCANNNNNTGG 1 cut(s) 737
PfoI TCCNGGA 1 cut(s) 895
PkrI GCNGC 4 cut(s) 487, 490, 654, 760
PleI GAGTC 4 cut(s) 149, 284, 476, 683
PluTI GGCGCC 1 cut(s) 72
PpsI GAGTC 4 cut(s) 149, 284, 476, 683
PpuMI RGGWCCY 1 cut(s) 572
PscI ACATGT 1 cut(s) 188
PshBI ATTAAT 1 cut(s) 204
Psp1406I AACGTT 1 cut(s) 618
Psp5II RGGWCCY 1 cut(s) 572
Psp6I CCWGG 3 cut(s) 64, 453, 895
PspGI CCWGG 3 cut(s) 64, 453, 895
PspN4I GGNNCC 5 cut(s) 63, 70, 406, 573, 894
PspPI GGNCC 3 cut(s) 16, 572, 893
PspPPI RGGWCCY 1 cut(s) 572
PstI CTGCAG 1 cut(s) 490
PstNI CAGNNNCTG 1 cut(s) 737
RsaI GTAC 5 cut(s) 326, 418, 563, 811, 846
RsaNI GTAC 5 cut(s) 325, 417, 562, 810, 845
RseI CAYNNNNRTG 2 cut(s) 542, 911
SapI GCTCTTC 1 cut(s) 928
SaqAI TTAA 3 cut(s) 204, 509, 885
SatI GCNGC 4 cut(s) 486, 489, 653, 759
Sau3AI GATC 6 cut(s) 96, 226, 412, 433, 499, 601
Sau96I GGNCC 3 cut(s) 16, 572, 893
ScaI AGTACT 1 cut(s) 326
SchI GAGTC 4 cut(s) 149, 284, 477, 683
ScrFI CCNGG 3 cut(s) 66, 455, 897
SduI GDGCHC 1 cut(s) 876
SfaNI GCATC 2 cut(s) 541, 745
SfcI CTRYAG 1 cut(s) 486
SfoI GGCGCC 1 cut(s) 70
SinI GGWCC 3 cut(s) 16, 572, 893
SmiMI CAYNNNNRTG 2 cut(s) 542, 911
Sse9I AATT 9 cut(s) 45, 88, 105, 159, 201, 510, 519, 626, 959
SseBI AGGCCT 1 cut(s) 481
SsiI CCGC 1 cut(s) 834
SspDI GGCGCC 1 cut(s) 68
SspI AATATT 1 cut(s) 585
SspMI CTAG 1 cut(s) 144
StuI AGGCCT 1 cut(s) 481
StyD4I CCNGG 3 cut(s) 64, 453, 895
StyI CCWWGG 2 cut(s) 472, 663
TaaI ACNGT 2 cut(s) 259, 421
TaiI ACGT 1 cut(s) 621
TaqI TCGA 3 cut(s) 125, 444, 956
TaqII GACCGA 1 cut(s) 33
TasI AATT 9 cut(s) 45, 88, 105, 159, 201, 510, 519, 626, 959
TatI WGTACW 1 cut(s) 324
TfiI GAWTC 1 cut(s) 743
Tru1I TTAA 3 cut(s) 204, 509, 885
Tru9I TTAA 3 cut(s) 204, 509, 885
TseFI GTSAC 2 cut(s) 136, 913
TseI GCWGC 4 cut(s) 485, 488, 652, 758
Tsp45I GTSAC 2 cut(s) 136, 913
TspDTI ATGAA 2 cut(s) 385, 668
TspGWI ACGGA 2 cut(s) 294, 580
Van91I CCANNNNNTGG 1 cut(s) 737
VpaK11BI GGWCC 3 cut(s) 16, 572, 893
VspI ATTAAT 1 cut(s) 204
XapI RAATTY 1 cut(s) 626
XceI RCATGY 1 cut(s) 192
XcmI CCANNNNNNNNNTGG 1 cut(s) 885
XspI CTAG 1 cut(s) 144
ZrmI AGTACT 1 cut(s) 326
Zsp2I ATGCAT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.