RLG00000016887

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005287
Physical Location & Seq
Forward (+)
10976432 .. 10978981
2550 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016887

Sequence Viewer

Length: 861 bp
ATGTCAAGCATTCCCCAAATGGCAGGTGGACCGGAAAGAGAAGATAAGGTCTCTTTGGAGCTGACGGAGGAGATTCTCCAAAGCATGGAAGTTGGGCTGGCTTTCAGAGACTACAATGGTAGAATCAGTTCGTTGGATTTTCACAAGGCTTCGAGTTATGTAGTGACTGCTAGTGATGATGAGTCGATTCGCTTGTATGATGTGTCCACTGCAACGTGCTTGAAGACCATTAACAGCAAAAAATATGGGGTTGATTTGGTTTGCTTTACTTCTCATCCTACCACGGTTATATACTCGTCGAAAAATGGCTGGGATGAATCGCTGAGGCTTCTCTCATTGAATGACAACAAGTACTTGCGCTATTTCAAAGGACATCATGACAGGGTTGTCTCCCTTAGCTTGTGCTCCCGCAAAGAGTGCTTCATTTCTGGTTCTCTTGATCGGACAGTTCTGCTTTGGGATCAACGAGCCGAGAAGTGCCAGAGTCTTTTACGTGTACAAGGAAGGCCTGCCACTGCTTATGATGACCAAGGGCTTGTTTTTACAATTGCCTTTGGAGGATACATACGAATGTTTGATGCCCGCATGTATGAAAAGGGTCCCTTTGATATCTTTTCTGTTGGGGGAGATATTTCTGATGCAAATGTTGTAAAGTTCAGTAATGATGGGAGACTTATGCTGTTAACAACGACAGATGGACATATTCATGTACTTGATTCATTCCGTGGCACGCTTTTATCCACGTACAATGTAAAGCCCGTTTCAAGCAATGCTACATTAGAGGCTTCTTTTAGCCCAGATGGGATGTTTGTTATGTCAGGTATACATGTCTCCTGTTACATATTTTGTTTTGGGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
WD40

Protein Analysis

287

Amino Acids

31.93

Weight (kDa)

5.9

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 25 - 162 1.8e-06 TEP-1 second beta-propeller
WD40_Prp19 PF24814 33 - 232 2.6e-18 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 35 - 102 9e-08 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 35 - 101 1.3e-07 WDR3 first beta-propeller domain
EIF3I PF24805 35 - 102 4.4e-06 EIF3I
WD40_CDC20-Fz PF24807 36 - 159 3.6e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 39 - 275 2.8e-32 WDR5 beta-propeller domain
WDR55 PF24796 41 - 161 1.8e-08 WDR55
Beta-prop_WDR90_POC16_2nd PF23393 50 - 169 2.2e-06 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 87 - 248 1.2e-10 WDHD1 first WD40 domain
NBCH_WD40 PF20426 102 - 159 1.4e-06 Neurobeachin beta propeller domain
Beta-prop_THOC3 PF25174 105 - 274 6e-14 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 105 - 165 7.5e-07 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 119 - 247 1.7e-08 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 132 - 273 8.1e-07 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 14
AasI GACNNNNNNGTC 1 cut(s) 386
Acc36I ACCTGC 1 cut(s) 14
AccB7I CCANNNNNTGG 1 cut(s) 85
AccI GTMKAC 1 cut(s) 823
AciI CCGC 2 cut(s) 409, 583
AclWI GGATC 1 cut(s) 468
AfaI GTAC 4 cut(s) 353, 498, 711, 746
AfiI CCNNNNNNNGG 1 cut(s) 85
AflIII ACRYGT 2 cut(s) 493, 826
AgsI TTSAA 4 cut(s) 223, 340, 367, 765
AluBI AGCT 2 cut(s) 61, 399
AluI AGCT 2 cut(s) 61, 399
Alw21I GWGCWC 1 cut(s) 407
Alw26I GTCTC 5 cut(s) 55, 102, 394, 664, 835
AlwI GGATC 1 cut(s) 468
AoxI GGCC 1 cut(s) 506
Asp700I GAANNNNTTC 1 cut(s) 127
AspLEI GCGC 1 cut(s) 360
AspS9I GGNCC 2 cut(s) 29, 599
AvaII GGWCC 2 cut(s) 29, 599
BaeGI GKGCMC 1 cut(s) 858
BbsI GAAGAC 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 407
BbvCI CCTCAGC 1 cut(s) 323
BccI CCATC 3 cut(s) 659, 689, 794
BciVI GTATCC 1 cut(s) 554
BcoDI GTCTC 5 cut(s) 55, 102, 394, 664, 835
BfaI CTAG 2 cut(s) 171, 859
BfuAI ACCTGC 1 cut(s) 14
BfuI GTATCC 1 cut(s) 554
BmcAI AGTACT 1 cut(s) 353
Bme18I GGWCC 2 cut(s) 29, 599
BmgT120I GGNCC 2 cut(s) 29, 599
BmiI GGNNCC 2 cut(s) 600, 601
BmsI GCATC 2 cut(s) 568, 628
BpiI GAAGAC 1 cut(s) 230
Bpu10I CCTNAGC 2 cut(s) 323, 395
BsaAI YACGTR 2 cut(s) 494, 744
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 3 cut(s) 282, 529, 724
BsaWI WCCGGW 1 cut(s) 31
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse3DI GCAATG 1 cut(s) 775
BseDI CCNNGG 3 cut(s) 282, 529, 724
BseGI GGATG 3 cut(s) 274, 319, 810
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 1 cut(s) 314
BseRI GAGGAG 1 cut(s) 83
BseSI GKGCMC 1 cut(s) 858
BseYI CCCAGC 1 cut(s) 309
BshFI GGCC 1 cut(s) 508
BsiHKAI GWGCWC 1 cut(s) 407
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 1 cut(s) 585
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 5 cut(s) 55, 102, 394, 664, 835
BsmFI GGGAC 1 cut(s) 585
BsmI GAATGC 1 cut(s) 9
BsnI GGCC 1 cut(s) 508
Bso31I GGTCTC 1 cut(s) 55
Bsp1286I GDGCHC 2 cut(s) 407, 858
Bsp1407I TGTACA 1 cut(s) 496
Bsp143I GATC 2 cut(s) 439, 460
BspACI CCGC 2 cut(s) 409, 583
BspANI GGCC 1 cut(s) 508
BspCNI CTCAG 1 cut(s) 315
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 2 cut(s) 600, 601
BspMI ACCTGC 1 cut(s) 14
BspPI GGATC 1 cut(s) 468
BspTNI GGTCTC 1 cut(s) 55
BsrDI GCAATG 1 cut(s) 775
BsrGI TGTACA 1 cut(s) 496
BssECI CCNNGG 3 cut(s) 282, 529, 724
BssMI GATC 2 cut(s) 439, 460
BssNAI GTATAC 1 cut(s) 824
BssT1I CCWWGG 1 cut(s) 529
Bst1107I GTATAC 1 cut(s) 824
Bst4CI ACNGT 2 cut(s) 286, 448
BstAPI GCANNNNNTGC 1 cut(s) 417
BstAUI TGTACA 1 cut(s) 496
BstBAI YACGTR 2 cut(s) 494, 744
BstC8I GCNNGC 4 cut(s) 99, 510, 583, 731
BstDEI CTNAG 2 cut(s) 323, 395
BstDSI CCRYGG 2 cut(s) 282, 724
BstF5I GGATG 3 cut(s) 274, 319, 810
BstHHI GCGC 1 cut(s) 360
BstKTI GATC 2 cut(s) 442, 463
BstMAI GTCTC 5 cut(s) 55, 102, 394, 664, 835
BstMBI GATC 2 cut(s) 439, 460
BstMWI GCNNNNNNNGC 1 cut(s) 417
BstNSI RCATGY 2 cut(s) 589, 830
BstSLI GKGCMC 1 cut(s) 858
BstV2I GAAGAC 1 cut(s) 230
BstZ17I GTATAC 1 cut(s) 824
BsuI GTATCC 1 cut(s) 554
BsuRI GGCC 1 cut(s) 508
BtgI CCRYGG 2 cut(s) 282, 724
BtsCI GGATG 3 cut(s) 274, 319, 810
BtsI GCAGTG 2 cut(s) 207, 513
BtsIMutI CAGTG 2 cut(s) 207, 513
BveI ACCTGC 1 cut(s) 14
Cac8I GCNNGC 4 cut(s) 99, 510, 583, 731
CciI TCATGA 1 cut(s) 376
CfoI GCGC 1 cut(s) 360
Cfr13I GGNCC 2 cut(s) 29, 599
Csp6I GTAC 4 cut(s) 352, 497, 710, 745
CviAII CATG 5 cut(s) 85, 377, 586, 707, 827
CviQI GTAC 4 cut(s) 352, 497, 710, 745
DdeI CTNAG 2 cut(s) 323, 395
DpnI GATC 2 cut(s) 441, 462
DpnII GATC 2 cut(s) 439, 460
DrdI GACNNNNNNGTC 1 cut(s) 386
DseDI GACNNNNNNGTC 1 cut(s) 386
Eco130I CCWWGG 1 cut(s) 529
Eco147I AGGCCT 1 cut(s) 508
Eco31I GGTCTC 1 cut(s) 55
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 2 cut(s) 29, 599
EcoO109I RGGNCCY 1 cut(s) 599
EcoRV GATATC 1 cut(s) 610
EcoT14I CCWWGG 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 529
FaeI CATG 5 cut(s) 88, 380, 589, 710, 830
FalI AAGNNNNNCTT 2 cut(s) 587, 619
FaqI GGGAC 1 cut(s) 585
FatI CATG 5 cut(s) 84, 376, 585, 706, 826
FauI CCCGC 2 cut(s) 416, 590
FblI GTMKAC 1 cut(s) 823
FokI GGATG 3 cut(s) 261, 326, 817
FspBI CTAG 2 cut(s) 171, 859
GlaI GCGC 1 cut(s) 359
GsaI CCCAGC 1 cut(s) 313
HaeIII GGCC 1 cut(s) 508
HapII CCGG 1 cut(s) 32
HhaI GCGC 1 cut(s) 360
Hin1II CATG 5 cut(s) 88, 380, 589, 710, 830
Hin6I GCGC 1 cut(s) 358
HinP1I GCGC 1 cut(s) 358
HincII GTYRAC 1 cut(s) 684
HindII GTYRAC 1 cut(s) 684
HinfI GANTC 7 cut(s) 73, 123, 182, 187, 317, 484, 716
HpaI GTTAAC 1 cut(s) 684
HpaII CCGG 1 cut(s) 32
Hpy166II GTNNAC 5 cut(s) 29, 207, 497, 684, 824
Hpy188I TCNGA 3 cut(s) 107, 444, 637
Hpy188III TCNNGA 2 cut(s) 377, 437
Hpy8I GTNNAC 5 cut(s) 29, 207, 497, 684, 824
Hpy99I CGWCG 1 cut(s) 301
HpyAV CCTTC 1 cut(s) 498
HpyCH4III ACNGT 2 cut(s) 286, 448
HpyCH4IV ACGT 3 cut(s) 215, 493, 743
HpyCH4V TGCA 2 cut(s) 212, 641
HpyF10VI GCNNNNNNNGC 1 cut(s) 417
HpyF3I CTNAG 2 cut(s) 323, 395
HpySE526I ACGT 3 cut(s) 215, 493, 743
Hsp92II CATG 5 cut(s) 88, 380, 589, 710, 830
HspAI GCGC 1 cut(s) 358
KflI GGGWCCC 1 cut(s) 599
KspAI GTTAAC 1 cut(s) 684
Kzo9I GATC 2 cut(s) 439, 460
LmnI GCTCC 2 cut(s) 58, 410
LweI GCATC 2 cut(s) 568, 628
MaeI CTAG 2 cut(s) 171, 859
MaeII ACGT 3 cut(s) 215, 493, 743
MaeIII GTNAC 2 cut(s) 163, 836
MalI GATC 2 cut(s) 441, 462
MboI GATC 2 cut(s) 439, 460
MboII GAAGA 2 cut(s) 53, 235
MfeI CAATTG 1 cut(s) 546
MhlI GDGCHC 2 cut(s) 407, 858
MluCI AATT 1 cut(s) 546
MlyI GAGTC 2 cut(s) 191, 493
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 4 cut(s) 61, 318, 551, 775
MroXI GAANNNNTTC 1 cut(s) 127
MseI TTAA 2 cut(s) 231, 683
MslI CAYNNNNRTG 2 cut(s) 569, 705
MspI CCGG 1 cut(s) 32
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 9
MwoI GCNNNNNNNGC 1 cut(s) 417
NdeII GATC 2 cut(s) 439, 460
NlaIII CATG 5 cut(s) 88, 380, 589, 710, 830
NlaIV GGNNCC 2 cut(s) 600, 601
NmeAIII GCCGAG 1 cut(s) 496
NmuCI GTSAC 1 cut(s) 163
NspI RCATGY 2 cut(s) 589, 830
PagI TCATGA 1 cut(s) 376
PaqCI CACCTGC 1 cut(s) 14
PceI AGGCCT 1 cut(s) 508
PciI ACATGT 1 cut(s) 826
PctI GAATGC 1 cut(s) 9
PdmI GAANNNNTTC 1 cut(s) 127
PfeI GAWTC 5 cut(s) 73, 123, 187, 317, 716
PflMI CCANNNNNTGG 1 cut(s) 85
PleI GAGTC 2 cut(s) 190, 492
PpsI GAGTC 2 cut(s) 190, 492
Ppu21I YACGTR 2 cut(s) 494, 744
PpuMI RGGWCCY 1 cut(s) 599
PscI ACATGT 1 cut(s) 826
Psp5II RGGWCCY 1 cut(s) 599
PspFI CCCAGC 1 cut(s) 309
PspN4I GGNNCC 2 cut(s) 600, 601
PspPI GGNCC 2 cut(s) 29, 599
PspPPI RGGWCCY 1 cut(s) 599
PsrI GAACNNNNNNTAC 2 cut(s) 112, 144
RsaI GTAC 4 cut(s) 353, 498, 711, 746
RsaNI GTAC 4 cut(s) 352, 497, 710, 745
RseI CAYNNNNRTG 2 cut(s) 569, 705
SaqAI TTAA 2 cut(s) 231, 683
Sau3AI GATC 2 cut(s) 439, 460
Sau96I GGNCC 2 cut(s) 29, 599
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 2 cut(s) 191, 493
SduI GDGCHC 2 cut(s) 407, 858
SetI ASST 8 cut(s) 28, 51, 63, 218, 401, 496, 746, 823
SfaNI GCATC 2 cut(s) 568, 628
SinI GGWCC 2 cut(s) 29, 599
SmiMI CAYNNNNRTG 2 cut(s) 569, 705
Sse9I AATT 1 cut(s) 546
SseBI AGGCCT 1 cut(s) 508
SsiI CCGC 2 cut(s) 409, 583
SspMI CTAG 2 cut(s) 171, 859
StuI AGGCCT 1 cut(s) 508
StyI CCWWGG 1 cut(s) 529
TaaI ACNGT 2 cut(s) 286, 448
TaiI ACGT 3 cut(s) 218, 496, 746
TaqI TCGA 3 cut(s) 152, 185, 299
TasI AATT 1 cut(s) 546
TatI WGTACW 3 cut(s) 351, 496, 709
TfiI GAWTC 5 cut(s) 73, 123, 187, 317, 716
Tru1I TTAA 2 cut(s) 231, 683
Tru9I TTAA 2 cut(s) 231, 683
TscAI CASTG 2 cut(s) 214, 520
TseFI GTSAC 1 cut(s) 163
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 5 cut(s) 330, 412, 606, 695, 708
TspGWI ACGGA 2 cut(s) 80, 713
TspRI CASTG 2 cut(s) 214, 520
Van91I CCANNNNNTGG 1 cut(s) 85
VpaK11BI GGWCC 2 cut(s) 29, 599
XceI RCATGY 2 cut(s) 589, 830
XcmI CCANNNNNNNNNTGG 1 cut(s) 23
XmiI GTMKAC 1 cut(s) 823
XmnI GAANNNNTTC 1 cut(s) 127
XspI CTAG 2 cut(s) 171, 859
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.