MD02G1132900.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005287
Physical Location & Seq
Forward (+)
10748120 .. 10751234
3115 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1132900.v1.1.491

Sequence Viewer

Length: 996 bp
ATGGCTGGGGGATCCGAAAGAGAAGATAAGGTCTCTCTGGAGCTGACGGAGGAAATTATTCAGAGCATGGAAGTTGGGTTAGCTTTCAGAGACTATAGTGGTAGAATTAGTTCATTGGACTTTCACAAGGCGTATAGTTATGTAGTGACTGCTAGTGATGACGAGTCGATTCGCCTGTATGATGTCTCCACTGCAACATGCTTGAAGACCATCAATAGCAAAAAGTATGGAGTTGATTTGGTTTGCTTTACTTCTCATCCGACAACCGTTATATACTCTTCCAAAAATGGCTGGGATGAATCCTTGAGGCTTCTCTCGTTGAATGACAATAAGTACTTGCGCTACTTCAAAGGCCACCATGACAGGGTAGTCTCGCTTAGCTTGTGTTCTCGTAAAGACTGCTTCATCTCGGGCTCTCTTGATAGAACTGTTCTGCTTTGGGATCAACGTGCCGAGAAATGCCAGGGTCTATTACGCGTTCAAGGAAGGCCTGCCACAGCTTATGATGATCAAGGACTAGTTTTTGCAATTGCCTTTGGTGGATACATAAGAATGTTTGATGCCAGAATGTACGAAAAGGGTCCTTTTGAAATCTTTTCTGTTGGGGGAGATATGTCAGATGCAAATGTTATCAAGTTCAGTAACGATGGGAGACTTATGCTGTTAACAACCACAGGTGGACATATTCATGTTCTTGATTCATTCCGTGGAACACTTTTATCCACATACAATGTAAAGCCAGTCTCCAGCAATTCAACGTTAGAGGCTTCTTTTAGCCCCGAGGGGATGTTTGTCATATCAGGTTCAGGTGATGGTAGTGTCTATGCTTGGAGTGTTCGGAGCGGGAAAGAAGTCACAAGCTGGAAGAGCACCGAAAATGAACCTCCAGTTATCAAATGGGCTCCTGGAAACCTAATGTTTGCAACTGGATCGTCCGAACTATCATTTTGGATTCCAGATTTGTCTAAATTGGGATCTTACGTTGGAAGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
WD40

Protein Analysis

332

Amino Acids

36.71

Weight (kDa)

6.11

Isoelectric Point (pI)

32.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 27 - 221 2.8e-18 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 29 - 95 3.6e-07 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 30 - 95 3.8e-07 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 30 - 153 2.9e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 33 - 313 8.6e-38 WDR5 beta-propeller domain
WDR55 PF24796 35 - 154 1.2e-07 WDR55
Beta-prop_WDR3_2nd PF25172 40 - 150 4.4e-10 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 45 - 163 6.7e-06 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 81 - 241 2e-09 WDHD1 first WD40 domain
NBCH_WD40 PF20426 96 - 151 3.1e-06 Neurobeachin beta propeller domain
WD40_Prp19 PF24814 97 - 315 3.7e-14 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 99 - 313 8.7e-20 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 99 - 161 1.6e-06 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 113 - 237 6.7e-06 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 126 - 291 2.7e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 196 - 323 3.6e-07 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
AccBSI CCGCTC 1 cut(s) 843
AccII CGCG 1 cut(s) 477
AciI CCGC 1 cut(s) 843
AclI AACGTT 1 cut(s) 758
AclWI GGATC 5 cut(s) 6, 19, 450, 937, 982
AfaI GTAC 2 cut(s) 335, 572
AfiI CCNNNNNNNGG 1 cut(s) 364
AflIII ACRYGT 1 cut(s) 475
AgsI TTSAA 6 cut(s) 205, 322, 349, 482, 590, 756
AhlI ACTAGT 1 cut(s) 517
AjnI CCWGG 2 cut(s) 462, 904
AluBI AGCT 5 cut(s) 43, 83, 381, 500, 861
AluI AGCT 5 cut(s) 43, 83, 381, 500, 861
Alw21I GWGCWC 1 cut(s) 872
Alw26I GTCTC 6 cut(s) 37, 84, 190, 376, 646, 748
AlwI GGATC 5 cut(s) 6, 19, 450, 937, 982
Ama87I CYCGRG 2 cut(s) 409, 779
AoxI GGCC 2 cut(s) 352, 488
Asp700I GAANNNNTTC 2 cut(s) 57, 109
AspLEI GCGC 1 cut(s) 342
AspS9I GGNCC 1 cut(s) 581
AsuHPI GGTGA 1 cut(s) 821
AvaI CYCGRG 2 cut(s) 409, 779
AvaII GGWCC 1 cut(s) 581
BamHI GGATCC 1 cut(s) 11
BanII GRGCYC 2 cut(s) 416, 904
BbsI GAAGAC 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 872
BccI CCATC 3 cut(s) 218, 641, 806
BcgI CGANNNNNNTGC 2 cut(s) 912, 946
BciT130I CCWGG 2 cut(s) 464, 906
BciVI GTATCC 1 cut(s) 536
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 6 cut(s) 37, 84, 190, 376, 646, 748
BcuI ACTAGT 1 cut(s) 517
BfaI CTAG 2 cut(s) 153, 518
BfmI CTRYAG 1 cut(s) 94
BfuI GTATCC 1 cut(s) 536
BlpI GCTNAGC 1 cut(s) 377
BmcAI AGTACT 1 cut(s) 335
Bme1390I CCNGG 2 cut(s) 464, 906
Bme18I GGWCC 1 cut(s) 581
BmeT110I CYCGRG 2 cut(s) 409, 779
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 3 cut(s) 13, 582, 903
BmrFI CCNGG 2 cut(s) 464, 906
BmsI GCATC 2 cut(s) 550, 610
BpiI GAAGAC 1 cut(s) 212
BpmI CTGGAG 3 cut(s) 59, 730, 870
Bpu1102I GCTNAGC 1 cut(s) 377
BpuEI CTTGAG 1 cut(s) 325
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 3 cut(s) 463, 706, 780
Bsc4I CCNNNNNNNGG 1 cut(s) 364
Bse1I ACTGG 3 cut(s) 740, 887, 931
BseBI CCWGG 2 cut(s) 464, 906
BseDI CCNNGG 3 cut(s) 463, 706, 780
BseGI GGATG 3 cut(s) 256, 301, 792
BseLI CCNNNNNNNGG 1 cut(s) 364
BseNI ACTGG 3 cut(s) 740, 887, 931
BseYI CCCAGC 2 cut(s) 5, 291
Bsh1236I CGCG 1 cut(s) 477
BshFI GGCC 2 cut(s) 354, 490
BsiHKAI GWGCWC 1 cut(s) 872
BsiHKCI CYCGRG 2 cut(s) 409, 779
BslI CCNNNNNNNGG 1 cut(s) 364
BsmAI GTCTC 6 cut(s) 37, 84, 190, 376, 646, 748
BsnI GGCC 2 cut(s) 354, 490
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 2 cut(s) 409, 779
Bsp1286I GDGCHC 3 cut(s) 416, 872, 904
Bsp143I GATC 5 cut(s) 11, 442, 508, 929, 974
Bsp1720I GCTNAGC 1 cut(s) 377
BspACI CCGC 1 cut(s) 843
BspANI GGCC 2 cut(s) 354, 490
BspFNI CGCG 1 cut(s) 477
BspLI GGNNCC 3 cut(s) 13, 582, 903
BspPI GGATC 5 cut(s) 6, 19, 450, 937, 982
BspQI GCTCTTC 1 cut(s) 860
BspTNI GGTCTC 1 cut(s) 37
BsrBI CCGCTC 1 cut(s) 843
BsrI ACTGG 3 cut(s) 740, 887, 931
BssECI CCNNGG 3 cut(s) 463, 706, 780
BssMI GATC 5 cut(s) 11, 442, 508, 929, 974
Bst2UI CCWGG 2 cut(s) 464, 906
Bst4CI ACNGT 2 cut(s) 268, 430
Bst6I CTCTTC 2 cut(s) 283, 860
BstC8I GCNNGC 1 cut(s) 492
BstDEI CTNAG 1 cut(s) 377
BstDSI CCRYGG 1 cut(s) 706
BstF5I GGATG 3 cut(s) 256, 301, 792
BstFNI CGCG 1 cut(s) 477
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 5 cut(s) 14, 445, 511, 932, 977
BstMAI GTCTC 6 cut(s) 37, 84, 190, 376, 646, 748
BstMBI GATC 5 cut(s) 11, 442, 508, 929, 974
BstMWI GCNNNNNNNGC 1 cut(s) 867
BstNI CCWGG 2 cut(s) 464, 906
BstNSI RCATGY 1 cut(s) 201
BstSCI CCNGG 2 cut(s) 462, 904
BstSFI CTRYAG 1 cut(s) 94
BstUI CGCG 1 cut(s) 477
BstV2I GAAGAC 1 cut(s) 212
BstX2I RGATCY 2 cut(s) 11, 974
BstYI RGATCY 2 cut(s) 11, 974
BsuI GTATCC 1 cut(s) 536
BsuRI GGCC 2 cut(s) 354, 490
BtgI CCRYGG 1 cut(s) 706
BtsCI GGATG 3 cut(s) 256, 301, 792
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 1 cut(s) 492
CfoI GCGC 1 cut(s) 342
Cfr13I GGNCC 1 cut(s) 581
Csp6I GTAC 2 cut(s) 334, 571
CviAII CATG 4 cut(s) 67, 198, 359, 689
CviQI GTAC 2 cut(s) 334, 571
DdeI CTNAG 1 cut(s) 377
DpnI GATC 5 cut(s) 13, 444, 510, 931, 976
DpnII GATC 5 cut(s) 11, 442, 508, 929, 974
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
Eam1104I CTCTTC 2 cut(s) 283, 860
EarI CTCTTC 2 cut(s) 283, 860
Eco147I AGGCCT 1 cut(s) 490
Eco24I GRGCYC 2 cut(s) 416, 904
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 581
Eco88I CYCGRG 2 cut(s) 409, 779
EcoO109I RGGNCCY 1 cut(s) 581
EcoRII CCWGG 2 cut(s) 462, 904
EcoT38I GRGCYC 2 cut(s) 416, 904
FaeI CATG 4 cut(s) 70, 201, 362, 692
FatI CATG 4 cut(s) 66, 197, 358, 688
FauI CCCGC 1 cut(s) 836
FbaI TGATCA 1 cut(s) 508
FokI GGATG 3 cut(s) 243, 308, 799
FriOI GRGCYC 2 cut(s) 416, 904
FspBI CTAG 2 cut(s) 153, 518
GlaI GCGC 1 cut(s) 341
GsaI CCCAGC 2 cut(s) 9, 295
GsuI CTGGAG 3 cut(s) 59, 730, 870
HaeIII GGCC 2 cut(s) 354, 490
HhaI GCGC 1 cut(s) 342
Hin1II CATG 4 cut(s) 70, 201, 362, 692
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HinfI GANTC 5 cut(s) 164, 169, 299, 698, 952
HpaI GTTAAC 1 cut(s) 666
HphI GGTGA 1 cut(s) 821
Hpy166II GTNNAC 2 cut(s) 666, 680
Hpy188I TCNGA 7 cut(s) 16, 63, 89, 261, 619, 840, 937
Hpy188III TCNNGA 4 cut(s) 38, 419, 695, 956
Hpy8I GTNNAC 2 cut(s) 666, 680
HpyAV CCTTC 1 cut(s) 480
HpyCH4III ACNGT 2 cut(s) 268, 430
HpyCH4IV ACGT 3 cut(s) 448, 758, 981
HpyCH4V TGCA 4 cut(s) 194, 527, 623, 923
HpyF10VI GCNNNNNNNGC 1 cut(s) 867
HpyF3I CTNAG 1 cut(s) 377
HpySE526I ACGT 3 cut(s) 448, 758, 981
Hsp92II CATG 4 cut(s) 70, 201, 362, 692
HspAI GCGC 1 cut(s) 340
Ksp22I TGATCA 1 cut(s) 508
KspAI GTTAAC 1 cut(s) 666
Kzo9I GATC 5 cut(s) 11, 442, 508, 929, 974
LguI GCTCTTC 1 cut(s) 860
LmnI GCTCC 3 cut(s) 40, 840, 907
LweI GCATC 2 cut(s) 550, 610
MaeI CTAG 2 cut(s) 153, 518
MaeII ACGT 3 cut(s) 448, 758, 981
MaeIII GTNAC 3 cut(s) 145, 641, 853
MalI GATC 5 cut(s) 13, 444, 510, 931, 976
MbiI CCGCTC 1 cut(s) 843
MboI GATC 5 cut(s) 11, 442, 508, 929, 974
MboII GAAGA 4 cut(s) 35, 217, 270, 877
MfeI CAATTG 1 cut(s) 528
MflI RGATCY 2 cut(s) 11, 974
MhlI GDGCHC 3 cut(s) 416, 872, 904
MluCI AATT 5 cut(s) 54, 105, 528, 751, 968
MluI ACGCGT 1 cut(s) 475
MlyI GAGTC 1 cut(s) 173
MmeI TCCRAC 2 cut(s) 284, 964
MnlI CCTC 5 cut(s) 43, 300, 757, 775, 894
MroXI GAANNNNTTC 2 cut(s) 57, 109
MseI TTAA 1 cut(s) 665
MslI CAYNNNNRTG 2 cut(s) 551, 687
MspR9I CCNGG 2 cut(s) 464, 906
MunI CAATTG 1 cut(s) 528
MvaI CCWGG 2 cut(s) 464, 906
MvnI CGCG 1 cut(s) 477
MwoI GCNNNNNNNGC 1 cut(s) 867
NdeII GATC 5 cut(s) 11, 442, 508, 929, 974
NlaIII CATG 4 cut(s) 70, 201, 362, 692
NlaIV GGNNCC 3 cut(s) 13, 582, 903
NmeAIII GCCGAG 1 cut(s) 478
NmuCI GTSAC 2 cut(s) 145, 853
NspI RCATGY 1 cut(s) 201
PceI AGGCCT 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 860
PdmI GAANNNNTTC 2 cut(s) 57, 109
PfeI GAWTC 4 cut(s) 169, 299, 698, 952
PfoI TCCNGGA 1 cut(s) 904
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PpuMI RGGWCCY 1 cut(s) 581
Psp1406I AACGTT 1 cut(s) 758
Psp5II RGGWCCY 1 cut(s) 581
Psp6I CCWGG 2 cut(s) 462, 904
PspFI CCCAGC 2 cut(s) 5, 291
PspGI CCWGG 2 cut(s) 462, 904
PspN4I GGNNCC 3 cut(s) 13, 582, 903
PspPI GGNCC 1 cut(s) 581
PspPPI RGGWCCY 1 cut(s) 581
PsrI GAACNNNNNNTAC 2 cut(s) 94, 126
PsuI RGATCY 2 cut(s) 11, 974
RsaI GTAC 2 cut(s) 335, 572
RsaNI GTAC 2 cut(s) 334, 571
RseI CAYNNNNRTG 2 cut(s) 551, 687
SapI GCTCTTC 1 cut(s) 860
SaqAI TTAA 1 cut(s) 665
Sau3AI GATC 5 cut(s) 11, 442, 508, 929, 974
Sau96I GGNCC 1 cut(s) 581
ScaI AGTACT 1 cut(s) 335
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 2 cut(s) 464, 906
SduI GDGCHC 3 cut(s) 416, 872, 904
SfaNI GCATC 2 cut(s) 550, 610
SfcI CTRYAG 1 cut(s) 94
SinI GGWCC 1 cut(s) 581
SmiMI CAYNNNNRTG 2 cut(s) 551, 687
SmlI CTYRAG 1 cut(s) 304
SmoI CTYRAG 1 cut(s) 304
SpeI ACTAGT 1 cut(s) 517
Sse9I AATT 5 cut(s) 54, 105, 528, 751, 968
SseBI AGGCCT 1 cut(s) 490
SsiI CCGC 1 cut(s) 843
SspMI CTAG 2 cut(s) 153, 518
StuI AGGCCT 1 cut(s) 490
StyD4I CCNGG 2 cut(s) 462, 904
TaaI ACNGT 2 cut(s) 268, 430
TaiI ACGT 3 cut(s) 451, 761, 984
TaqI TCGA 1 cut(s) 167
TasI AATT 5 cut(s) 54, 105, 528, 751, 968
TatI WGTACW 1 cut(s) 333
TfiI GAWTC 4 cut(s) 169, 299, 698, 952
Tru1I TTAA 1 cut(s) 665
Tru9I TTAA 1 cut(s) 665
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 2 cut(s) 145, 853
Tsp45I GTSAC 2 cut(s) 145, 853
TspDTI ATGAA 6 cut(s) 102, 312, 394, 677, 690, 894
TspGWI ACGGA 2 cut(s) 62, 695
TspRI CASTG 1 cut(s) 196
VpaK11BI GGWCC 1 cut(s) 581
XceI RCATGY 1 cut(s) 201
XcmI CCANNNNNNNNNTGG 1 cut(s) 894
XmnI GAANNNNTTC 2 cut(s) 57, 109
XspI CTAG 2 cut(s) 153, 518
ZrmI AGTACT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.