FvH4_1g11900

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005287
Physical Location & Seq
Forward (+)
6503517 .. 6506205
2689 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g11900.t1

Sequence Viewer

Length: 999 bp
ATGGCAGGTGTACCGGAAAGAGAAGACAAGGTCTCTCTGGAGCTGACGGAGGAAATTCTCCAGAGCATGGAAGTCGGGCTCGCTTTCCGAGACTACAGTGGTAGAATCAGTTCGTTGGATTTTCACAAGGCTTCGAGTTACGTAGTGACTGCTAGTGATGATGAGTCGATTCGGTTATATGATGTCTCCACTGCGACGTGCTTGAAGACAATTAACAGCAAAAAGTATGGGGTTGATTTGGTTTGCTTTACTTCTCATCCTACCACGGTTATATACTCTTCGAAAAATGGCTGGGATGAATCGCTGAGGCTTCTCTCGTTGAATGACAATAAGTACTTGCGCTATTTCAAAGGCCATCACGACAGGGTTGTCTCACTTAGTTTGTGCTCCCGGAAAGAGTGCTTCATCTCTGGTTCTCTTGATAGGACAGTTATGCTTTGGGATCAACGAGTCGAGAAATGTCAGGGTCTTTTACATGTACAAGGAAGGCCTGCTACAGCTTACGATGACCAAGGGCTAGTTTTTACAGTTGCATTTGGTGGTTACATACGAATGTTTGACGCCCGCATGTATGAAAAGGGTCCCTTTGATATATTTTCTGTTGGGGGAGACATGTCTGATGCAAATGTTGTAAAGTTCAGTAATGATGGGAGACTTATGCTGTTAACAACTACAGATGGACATATTCATGTACTTGATTCGTTCCGCGGCACGCTTTTATCCACTTACAATGTAAAGCCCGTGTCAAGCACTGCTACATTAGAGGCTTCTTTTAGCCCAGATGGGATGTTTGTTATGTCAGGTTCAGGTGATGGTAGTGTCTATGCTTGGAGTGTTCGAAGCGGGAAAGAAGTCACAAGTTGGCTCAGTACTGAAAGTGAACCTCCTGTTATCAAATGGGCTCCAGGAAGCCTCATGTTTGCAACTGGTTCTTCTGAACTATCATTTTGGATACCGGATTTGTCTAAACTGGCAGCTTATGTTGGTGGAAGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
WD40

Protein Analysis

333

Amino Acids

36.73

Weight (kDa)

5.69

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 19 - 150 4.7e-06 TEP-1 second beta-propeller
WD40_Prp19 PF24814 27 - 226 2.3e-18 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 29 - 96 1.6e-07 THOC3 beta-propeller domain
EIF3I PF24805 29 - 96 9.9e-06 EIF3I
Beta-prop_WDR3_1st PF25173 30 - 95 2e-07 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 30 - 153 7.4e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 33 - 312 3.1e-39 WDR5 beta-propeller domain
WDR55 PF24796 35 - 154 4.2e-08 WDR55
Beta-prop_WDR3_2nd PF25172 38 - 151 5.3e-11 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 81 - 241 2.1e-10 WDHD1 first WD40 domain
NBCH_WD40 PF20426 97 - 150 4.5e-07 Neurobeachin beta propeller domain
Beta-prop_WDR3_1st PF25173 99 - 154 2.1e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 99 - 313 1.4e-19 THOC3 beta-propeller domain
WD40 PF00400 111 - 148 6.1e-06 WD domain, G-beta repeat
WD40_Gbeta PF25391 112 - 237 9.5e-08 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 126 - 288 1.7e-12 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 180 - 289 4.4e-06 WDR55
WD40_CDC20-Fz PF24807 204 - 324 3.4e-07 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 207 - 288 1e-05 WDHD1 first WD40 domain
WD40_Prp19 PF24814 211 - 316 7.4e-07 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
AccB7I CCANNNNNTGG 1 cut(s) 67
AccII CGCG 1 cut(s) 708
AciI CCGC 4 cut(s) 565, 706, 708, 843
AclWI GGATC 1 cut(s) 450
AcsI RAATTY 1 cut(s) 54
AcyI GRCGYC 1 cut(s) 561
AfaI GTAC 5 cut(s) 12, 335, 480, 693, 871
AfiI CCNNNNNNNGG 1 cut(s) 67
AflIII ACRYGT 2 cut(s) 475, 612
AgsI TTSAA 3 cut(s) 205, 322, 349
AjiI CACGTC 1 cut(s) 198
AjnI CCWGG 1 cut(s) 904
AluBI AGCT 3 cut(s) 43, 500, 977
AluI AGCT 3 cut(s) 43, 500, 977
Alw21I GWGCWC 1 cut(s) 389
Alw26I GTCTC 6 cut(s) 37, 84, 190, 376, 603, 646
AlwI GGATC 1 cut(s) 450
AoxI GGCC 2 cut(s) 352, 488
ApeKI GCWGC 1 cut(s) 974
ApoI RAATTY 1 cut(s) 54
Asp700I GAANNNNTTC 1 cut(s) 109
AspLEI GCGC 1 cut(s) 342
AspS9I GGNCC 1 cut(s) 581
AsuC2I CCSGG 1 cut(s) 391
AsuHPI GGTGA 1 cut(s) 821
AsuII TTCGAA 2 cut(s) 281, 838
AvaII GGWCC 1 cut(s) 581
BanII GRGCYC 2 cut(s) 81, 904
BarI GAAGNNNNNNTAC 2 cut(s) 478, 510
BbsI GAAGAC 2 cut(s) 30, 212
Bbv12I GWGCWC 1 cut(s) 389
BbvCI CCTCAGC 1 cut(s) 305
BbvI GCAGC 1 cut(s) 986
BccI CCATC 5 cut(s) 363, 641, 671, 776, 806
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 1 cut(s) 906
BciVI GTATCC 1 cut(s) 945
BcnI CCSGG 1 cut(s) 391
BcoDI GTCTC 6 cut(s) 37, 84, 190, 376, 603, 646
BfaI CTAG 2 cut(s) 153, 518
BfmI CTRYAG 3 cut(s) 94, 495, 672
BfuI GTATCC 1 cut(s) 945
BisI GCNGC 2 cut(s) 709, 975
BlsI GCNGC 2 cut(s) 710, 976
BmcAI AGTACT 2 cut(s) 335, 871
Bme1390I CCNGG 2 cut(s) 391, 906
Bme18I GGWCC 1 cut(s) 581
BmgBI CACGTC 1 cut(s) 198
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 3 cut(s) 582, 583, 903
BmrFI CCNGG 2 cut(s) 391, 906
BmsI GCATC 1 cut(s) 610
BpiI GAAGAC 2 cut(s) 30, 212
BpmI CTGGAG 3 cut(s) 44, 59, 888
Bpu10I CCTNAGC 1 cut(s) 305
Bpu14I TTCGAA 2 cut(s) 281, 838
BpuMI CCSGG 1 cut(s) 391
BsaAI YACGTR 1 cut(s) 142
BsaHI GRCGYC 1 cut(s) 561
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 3 cut(s) 264, 511, 706
BsaWI WCCGGW 2 cut(s) 13, 955
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 2 cut(s) 931, 975
BseBI CCWGG 1 cut(s) 906
BseDI CCNNGG 3 cut(s) 264, 511, 706
BseGI GGATG 3 cut(s) 256, 301, 792
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMII CTCAG 2 cut(s) 296, 880
BseNI ACTGG 2 cut(s) 931, 975
BseXI GCAGC 1 cut(s) 986
BseYI CCCAGC 1 cut(s) 291
Bsh1236I CGCG 1 cut(s) 708
BshFI GGCC 2 cut(s) 354, 490
BsiHKAI GWGCWC 1 cut(s) 389
BsiSI CCGG 3 cut(s) 14, 391, 956
BslFI GGGAC 1 cut(s) 567
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 6 cut(s) 37, 84, 190, 376, 603, 646
BsmFI GGGAC 1 cut(s) 567
BsnI GGCC 2 cut(s) 354, 490
Bso31I GGTCTC 1 cut(s) 37
Bsp119I TTCGAA 2 cut(s) 281, 838
Bsp1286I GDGCHC 3 cut(s) 81, 389, 904
Bsp1407I TGTACA 1 cut(s) 478
Bsp143I GATC 1 cut(s) 442
BspACI CCGC 4 cut(s) 565, 706, 708, 843
BspANI GGCC 2 cut(s) 354, 490
BspCNI CTCAG 2 cut(s) 297, 879
BspFNI CGCG 1 cut(s) 708
BspLI GGNNCC 3 cut(s) 582, 583, 903
BspPI GGATC 1 cut(s) 450
BspT104I TTCGAA 2 cut(s) 281, 838
BspTNI GGTCTC 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 478
BsrI ACTGG 2 cut(s) 931, 975
BssECI CCNNGG 3 cut(s) 264, 511, 706
BssMI GATC 1 cut(s) 442
BssNI GRCGYC 1 cut(s) 561
BssT1I CCWWGG 1 cut(s) 511
Bst2UI CCWGG 1 cut(s) 906
Bst4CI ACNGT 4 cut(s) 98, 268, 430, 529
Bst6I CTCTTC 1 cut(s) 283
BstACI GRCGYC 1 cut(s) 561
BstAUI TGTACA 1 cut(s) 478
BstBAI YACGTR 1 cut(s) 142
BstBI TTCGAA 2 cut(s) 281, 838
BstC8I GCNNGC 4 cut(s) 81, 492, 565, 713
BstDEI CTNAG 3 cut(s) 305, 377, 866
BstDSI CCRYGG 2 cut(s) 264, 706
BstF5I GGATG 3 cut(s) 256, 301, 792
BstFNI CGCG 1 cut(s) 708
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 1 cut(s) 445
BstMAI GTCTC 6 cut(s) 37, 84, 190, 376, 603, 646
BstMBI GATC 1 cut(s) 442
BstNI CCWGG 1 cut(s) 906
BstNSI RCATGY 3 cut(s) 479, 571, 616
BstSCI CCNGG 2 cut(s) 389, 904
BstSFI CTRYAG 3 cut(s) 94, 495, 672
BstSNI TACGTA 1 cut(s) 142
BstUI CGCG 1 cut(s) 708
BstV1I GCAGC 1 cut(s) 986
BstV2I GAAGAC 2 cut(s) 30, 212
BsuI GTATCC 1 cut(s) 945
BsuRI GGCC 2 cut(s) 354, 490
BtgI CCRYGG 2 cut(s) 264, 706
BtrI CACGTC 1 cut(s) 198
BtsCI GGATG 3 cut(s) 256, 301, 792
BtsI GCAGTG 2 cut(s) 189, 750
BtsIMutI CAGTG 3 cut(s) 103, 189, 750
Cac8I GCNNGC 4 cut(s) 81, 492, 565, 713
CfoI GCGC 1 cut(s) 342
Cfr13I GGNCC 1 cut(s) 581
Cfr42I CCGCGG 1 cut(s) 709
CseI GACGC 1 cut(s) 569
Csp6I GTAC 5 cut(s) 11, 334, 479, 692, 870
CviAII CATG 6 cut(s) 67, 476, 568, 613, 689, 916
CviQI GTAC 5 cut(s) 11, 334, 479, 692, 870
DdeI CTNAG 3 cut(s) 305, 377, 866
DpnI GATC 1 cut(s) 444
DpnII GATC 1 cut(s) 442
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
Eam1104I CTCTTC 1 cut(s) 283
EarI CTCTTC 1 cut(s) 283
Eco105I TACGTA 1 cut(s) 142
Eco130I CCWWGG 1 cut(s) 511
Eco147I AGGCCT 1 cut(s) 490
Eco24I GRGCYC 2 cut(s) 81, 904
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 581
EcoO109I RGGNCCY 1 cut(s) 581
EcoRII CCWGG 1 cut(s) 904
EcoT14I CCWWGG 1 cut(s) 511
EcoT38I GRGCYC 2 cut(s) 81, 904
ErhI CCWWGG 1 cut(s) 511
FaeI CATG 6 cut(s) 70, 479, 571, 616, 692, 919
FalI AAGNNNNNCTT 2 cut(s) 569, 601
FaqI GGGAC 1 cut(s) 567
FatI CATG 6 cut(s) 66, 475, 567, 612, 688, 915
FauI CCCGC 2 cut(s) 572, 836
Fnu4HI GCNGC 2 cut(s) 709, 975
FokI GGATG 3 cut(s) 243, 308, 799
FriOI GRGCYC 2 cut(s) 81, 904
Fsp4HI GCNGC 2 cut(s) 709, 975
FspBI CTAG 2 cut(s) 153, 518
GlaI GCGC 1 cut(s) 341
GluI GCNGC 2 cut(s) 709, 975
GsaI CCCAGC 1 cut(s) 295
GsuI CTGGAG 3 cut(s) 44, 59, 888
HaeIII GGCC 2 cut(s) 354, 490
HapII CCGG 3 cut(s) 14, 391, 956
HgaI GACGC 1 cut(s) 569
HhaI GCGC 1 cut(s) 342
Hin1I GRCGYC 1 cut(s) 561
Hin1II CATG 6 cut(s) 70, 479, 571, 616, 692, 919
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HinfI GANTC 6 cut(s) 105, 164, 169, 299, 450, 698
HpaI GTTAAC 1 cut(s) 666
HpaII CCGG 3 cut(s) 14, 391, 956
HphI GGTGA 1 cut(s) 821
Hpy166II GTNNAC 3 cut(s) 11, 666, 881
Hpy188I TCNGA 3 cut(s) 89, 619, 937
Hpy188III TCNNGA 5 cut(s) 38, 61, 359, 419, 454
Hpy8I GTNNAC 3 cut(s) 11, 666, 881
Hpy99I CGWCG 1 cut(s) 199
HpyAV CCTTC 2 cut(s) 480, 984
HpyCH4III ACNGT 4 cut(s) 98, 268, 430, 529
HpyCH4IV ACGT 2 cut(s) 141, 197
HpyCH4V TGCA 3 cut(s) 533, 623, 923
HpyF3I CTNAG 3 cut(s) 305, 377, 866
HpySE526I ACGT 2 cut(s) 141, 197
Hsp92I GRCGYC 1 cut(s) 561
Hsp92II CATG 6 cut(s) 70, 479, 571, 616, 692, 919
HspAI GCGC 1 cut(s) 340
KflI GGGWCCC 1 cut(s) 581
KspAI GTTAAC 1 cut(s) 666
KspI CCGCGG 1 cut(s) 709
Kzo9I GATC 1 cut(s) 442
LmnI GCTCC 3 cut(s) 40, 392, 907
Lsp1109I GCAGC 1 cut(s) 986
LweI GCATC 1 cut(s) 610
MaeI CTAG 2 cut(s) 153, 518
MaeII ACGT 2 cut(s) 141, 197
MaeIII GTNAC 4 cut(s) 137, 145, 542, 853
MalI GATC 1 cut(s) 444
MboI GATC 1 cut(s) 442
MboII GAAGA 4 cut(s) 35, 217, 270, 924
MhlI GDGCHC 3 cut(s) 81, 389, 904
MluCI AATT 2 cut(s) 54, 210
MlyI GAGTC 2 cut(s) 173, 459
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 5 cut(s) 43, 300, 757, 894, 923
MroXI GAANNNNTTC 1 cut(s) 109
MseI TTAA 2 cut(s) 213, 665
MslI CAYNNNNRTG 2 cut(s) 551, 687
MspA1I CMGCKG 1 cut(s) 708
MspI CCGG 3 cut(s) 14, 391, 956
MspR9I CCNGG 2 cut(s) 391, 906
MvaI CCWGG 1 cut(s) 906
MvnI CGCG 1 cut(s) 708
NciI CCSGG 1 cut(s) 391
NdeII GATC 1 cut(s) 442
NlaIII CATG 6 cut(s) 70, 479, 571, 616, 692, 919
NlaIV GGNNCC 3 cut(s) 582, 583, 903
NmuCI GTSAC 2 cut(s) 145, 853
NspI RCATGY 3 cut(s) 479, 571, 616
NspV TTCGAA 2 cut(s) 281, 838
PceI AGGCCT 1 cut(s) 490
PciI ACATGT 2 cut(s) 475, 612
PdmI GAANNNNTTC 1 cut(s) 109
PfeI GAWTC 4 cut(s) 105, 169, 299, 698
PflFI GACNNNGTC 1 cut(s) 29
PflMI CCANNNNNTGG 1 cut(s) 67
PfoI TCCNGGA 2 cut(s) 389, 904
PkrI GCNGC 2 cut(s) 710, 976
PleI GAGTC 2 cut(s) 172, 458
PpsI GAGTC 2 cut(s) 172, 458
Ppu21I YACGTR 1 cut(s) 142
PpuMI RGGWCCY 1 cut(s) 581
PscI ACATGT 2 cut(s) 475, 612
Psp5II RGGWCCY 1 cut(s) 581
Psp6I CCWGG 1 cut(s) 904
PspFI CCCAGC 1 cut(s) 291
PspGI CCWGG 1 cut(s) 904
PspN4I GGNNCC 3 cut(s) 582, 583, 903
PspPI GGNCC 1 cut(s) 581
PspPPI RGGWCCY 1 cut(s) 581
PsrI GAACNNNNNNTAC 2 cut(s) 94, 126
PsyI GACNNNGTC 1 cut(s) 29
RsaI GTAC 5 cut(s) 12, 335, 480, 693, 871
RsaNI GTAC 5 cut(s) 11, 334, 479, 692, 870
RseI CAYNNNNRTG 2 cut(s) 551, 687
SacII CCGCGG 1 cut(s) 709
SaqAI TTAA 2 cut(s) 213, 665
SatI GCNGC 2 cut(s) 709, 975
Sau3AI GATC 1 cut(s) 442
Sau96I GGNCC 1 cut(s) 581
ScaI AGTACT 2 cut(s) 335, 871
SchI GAGTC 2 cut(s) 173, 459
ScrFI CCNGG 2 cut(s) 391, 906
SduI GDGCHC 3 cut(s) 81, 389, 904
SfaNI GCATC 1 cut(s) 610
SfcI CTRYAG 3 cut(s) 94, 495, 672
Sfr303I CCGCGG 1 cut(s) 709
SfuI TTCGAA 2 cut(s) 281, 838
SgrBI CCGCGG 1 cut(s) 709
SinI GGWCC 1 cut(s) 581
SmiMI CAYNNNNRTG 2 cut(s) 551, 687
SnaBI TACGTA 1 cut(s) 142
Sse9I AATT 2 cut(s) 54, 210
SseBI AGGCCT 1 cut(s) 490
SsiI CCGC 4 cut(s) 565, 706, 708, 843
SspMI CTAG 2 cut(s) 153, 518
StuI AGGCCT 1 cut(s) 490
StyD4I CCNGG 2 cut(s) 389, 904
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 4 cut(s) 98, 268, 430, 529
TaiI ACGT 2 cut(s) 144, 200
TaqI TCGA 5 cut(s) 134, 167, 281, 453, 838
TasI AATT 2 cut(s) 54, 210
TatI WGTACW 4 cut(s) 333, 478, 691, 869
TauI GCSGC 1 cut(s) 711
TfiI GAWTC 4 cut(s) 105, 169, 299, 698
Tru1I TTAA 2 cut(s) 213, 665
Tru9I TTAA 2 cut(s) 213, 665
TscAI CASTG 3 cut(s) 103, 196, 757
TseFI GTSAC 2 cut(s) 145, 853
TseI GCWGC 1 cut(s) 974
Tsp45I GTSAC 2 cut(s) 145, 853
TspDTI ATGAA 4 cut(s) 312, 394, 588, 677
TspGWI ACGGA 1 cut(s) 62
TspRI CASTG 3 cut(s) 103, 196, 757
Tth111I GACNNNGTC 1 cut(s) 29
Van91I CCANNNNNTGG 1 cut(s) 67
VpaK11BI GGWCC 1 cut(s) 581
XapI RAATTY 1 cut(s) 54
XceI RCATGY 3 cut(s) 479, 571, 616
XmnI GAANNNNTTC 1 cut(s) 109
XspI CTAG 2 cut(s) 153, 518
ZrmI AGTACT 2 cut(s) 335, 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.