Rroxscaffold_2G00143030

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005287
Physical Location & Seq
Reverse (-)
81057611 .. 81060144
2534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00143030.1

Sequence Viewer

Length: 1017 bp
ATGTCAAGCATTCCCCAAATGGCAGGTGGACCGGAGAGAGAAGATAAGGTCTCTTTGGAGCTGACGGAGGAGATTCTCCAAAGCATGGAAGTTGGGCTGGCTTTCAGAGACTACAATGGTAGAATCAGTTCGTTGGATTTTCACAAGGCTTCGAGTTATGTAGTGACTGCTAGTGATGATGAGTCGATTCGCTTGTATGATGTGTCCACTGCAACGTGCTTGAAGACCATTAACAGCAAAAAATATGGGGTTGATTTGGTCTGCTTTACTTCTCATCCTACCACGGTTATATACTCGTCGAAAAATGGCTGGGATGAATCGCTGAGGCTTCTCTCATTGAATGACAACAAGTACTTGCGCTATTTCAAAGGACATCATGACAGGGTTGTCTCACTTAGCTTGTGCTCCCGCAAAGAGTGCTTCATTTCTGGTTCTCTTGATCGGACAGTTCTGCTTTGGGATCAACGAGCCGAGAAGTGCCAGGGTCTTTTACGTGTACAAGGAAGGCCAGCCACTGCTTATGATGACCAAGGGCTTGTTTTTACAATTGCCTTTGGTGGATACATACGAATGTTTGACGCCCGCATGTATGAAAAGGGTCCCTTTGATATCTTTTCTGTTGGGGGAGATATGTCTGATGCAAATGTTGTAAAGTTCAGTAATGATGGGAGACTTATGCTGTTAACAACGACAGATGGACATATTCATGTACTTGATTCATTCCGTGGCACGCTTTTATCCACGTACAATGTAAAGCCCGTTTCAAGCAATGCTACATTAGAGGCTTCTTTTAGCCCAGATGGGATGTTTGTTATGTCAGGTTCAGGTGATGGTAGTGTCTATGCTTGGAGTGTTCGCAGCGGGAAAGAAGTCACAAGTTGGCTTAGTACTGAAAGTGAACCTCCTGTTATTAAATGGGCTCCTGGAAGCCTCATGTTTGCAACTGGGTCTTCTGAACTATCATTTTGGATTCCGGATTTGTCTAAACTGGCAGCTTATGTTGGTGGAAGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
WD40

Protein Analysis

338

Amino Acids

37.36

Weight (kDa)

5.74

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 25 - 157 4.8e-06 TEP-1 second beta-propeller
WD40_Prp19 PF24814 33 - 232 4.9e-18 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 35 - 102 9.8e-08 THOC3 beta-propeller domain
EIF3I PF24805 35 - 102 5.7e-06 EIF3I
Beta-prop_WDR3_1st PF25173 36 - 101 1.8e-07 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 36 - 159 8.2e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 39 - 318 3.4e-39 WDR5 beta-propeller domain
WDR55 PF24796 41 - 160 3.3e-08 WDR55
Beta-prop_WDR3_2nd PF25172 44 - 157 9.3e-11 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 50 - 168 5.6e-06 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 87 - 247 4.6e-10 WDHD1 first WD40 domain
NBCH_WD40 PF20426 102 - 158 2.7e-06 Neurobeachin beta propeller domain
Beta-prop_WDR3_1st PF25173 105 - 168 1.2e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 105 - 318 1.5e-19 THOC3 beta-propeller domain
WD40_Gbeta PF25391 119 - 243 3.1e-07 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 132 - 294 3e-12 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 187 - 295 6.5e-06 WDR55
WD40_CDC20-Fz PF24807 211 - 330 5.2e-07 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 214 - 295 8.9e-06 WDHD1 first WD40 domain
WD40_Prp19 PF24814 215 - 323 4.9e-07 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 14
AasI GACNNNNNNGTC 1 cut(s) 386
Acc36I ACCTGC 1 cut(s) 14
AccB7I CCANNNNNTGG 1 cut(s) 85
AccIII TCCGGA 1 cut(s) 973
AciI CCGC 3 cut(s) 409, 583, 861
AclWI GGATC 1 cut(s) 468
AcyI GRCGYC 1 cut(s) 579
AfaI GTAC 5 cut(s) 353, 498, 711, 746, 889
AfiI CCNNNNNNNGG 1 cut(s) 85
AflIII ACRYGT 1 cut(s) 493
AgsI TTSAA 4 cut(s) 223, 340, 367, 765
AjnI CCWGG 2 cut(s) 480, 922
AluBI AGCT 3 cut(s) 61, 399, 995
AluI AGCT 3 cut(s) 61, 399, 995
Alw21I GWGCWC 1 cut(s) 407
Alw26I GTCTC 4 cut(s) 55, 102, 394, 664
AlwI GGATC 1 cut(s) 468
AlwNI CAGNNNCTG 1 cut(s) 515
Aor13HI TCCGGA 1 cut(s) 973
AoxI GGCC 1 cut(s) 506
ApeKI GCWGC 2 cut(s) 858, 992
Asp700I GAANNNNTTC 1 cut(s) 127
AspLEI GCGC 1 cut(s) 360
AspS9I GGNCC 2 cut(s) 29, 599
AsuHPI GGTGA 1 cut(s) 839
AvaII GGWCC 2 cut(s) 29, 599
BanII GRGCYC 1 cut(s) 922
BbsI GAAGAC 2 cut(s) 230, 942
Bbv12I GWGCWC 1 cut(s) 407
BbvCI CCTCAGC 1 cut(s) 323
BbvI GCAGC 2 cut(s) 870, 1004
BccI CCATC 4 cut(s) 659, 689, 794, 824
BciT130I CCWGG 2 cut(s) 482, 924
BciVI GTATCC 1 cut(s) 554
BcoDI GTCTC 4 cut(s) 55, 102, 394, 664
BfaI CTAG 1 cut(s) 171
BfuAI ACCTGC 1 cut(s) 14
BfuI GTATCC 1 cut(s) 554
BisI GCNGC 2 cut(s) 859, 993
BlsI GCNGC 2 cut(s) 860, 994
BmcAI AGTACT 2 cut(s) 353, 889
Bme1390I CCNGG 2 cut(s) 482, 924
Bme18I GGWCC 2 cut(s) 29, 599
BmgT120I GGNCC 2 cut(s) 29, 599
BmiI GGNNCC 3 cut(s) 600, 601, 921
BmrFI CCNGG 2 cut(s) 482, 924
BmrI ACTGGG 1 cut(s) 954
BmsI GCATC 1 cut(s) 628
BmuI ACTGGG 1 cut(s) 954
BpiI GAAGAC 2 cut(s) 230, 942
Bpu10I CCTNAGC 1 cut(s) 323
BsaAI YACGTR 2 cut(s) 494, 744
BsaHI GRCGYC 1 cut(s) 579
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 4 cut(s) 282, 481, 529, 724
BsaWI WCCGGW 2 cut(s) 31, 973
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse1I ACTGG 2 cut(s) 949, 993
Bse3DI GCAATG 1 cut(s) 775
BseAI TCCGGA 1 cut(s) 973
BseBI CCWGG 2 cut(s) 482, 924
BseDI CCNNGG 4 cut(s) 282, 481, 529, 724
BseGI GGATG 3 cut(s) 274, 319, 810
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 2 cut(s) 949, 993
BseRI GAGGAG 1 cut(s) 83
BseXI GCAGC 2 cut(s) 870, 1004
BseYI CCCAGC 1 cut(s) 309
BshFI GGCC 1 cut(s) 508
BsiHKAI GWGCWC 1 cut(s) 407
BsiSI CCGG 2 cut(s) 32, 974
BslFI GGGAC 1 cut(s) 585
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 4 cut(s) 55, 102, 394, 664
BsmFI GGGAC 1 cut(s) 585
BsmI GAATGC 1 cut(s) 9
BsnI GGCC 1 cut(s) 508
Bso31I GGTCTC 1 cut(s) 55
Bsp1286I GDGCHC 2 cut(s) 407, 922
Bsp13I TCCGGA 1 cut(s) 973
Bsp1407I TGTACA 1 cut(s) 496
Bsp143I GATC 2 cut(s) 439, 460
BspACI CCGC 3 cut(s) 409, 583, 861
BspANI GGCC 1 cut(s) 508
BspCNI CTCAG 1 cut(s) 315
BspEI TCCGGA 1 cut(s) 973
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 3 cut(s) 600, 601, 921
BspMI ACCTGC 1 cut(s) 14
BspPI GGATC 1 cut(s) 468
BspTNI GGTCTC 1 cut(s) 55
BsrDI GCAATG 1 cut(s) 775
BsrGI TGTACA 1 cut(s) 496
BsrI ACTGG 2 cut(s) 949, 993
BssECI CCNNGG 4 cut(s) 282, 481, 529, 724
BssMI GATC 2 cut(s) 439, 460
BssNI GRCGYC 1 cut(s) 579
BssT1I CCWWGG 1 cut(s) 529
Bst2UI CCWGG 2 cut(s) 482, 924
Bst4CI ACNGT 2 cut(s) 286, 448
BstACI GRCGYC 1 cut(s) 579
BstAPI GCANNNNNTGC 1 cut(s) 417
BstAUI TGTACA 1 cut(s) 496
BstBAI YACGTR 2 cut(s) 494, 744
BstC8I GCNNGC 4 cut(s) 99, 510, 583, 731
BstDEI CTNAG 3 cut(s) 323, 395, 884
BstDSI CCRYGG 2 cut(s) 282, 724
BstF5I GGATG 3 cut(s) 274, 319, 810
BstHHI GCGC 1 cut(s) 360
BstKTI GATC 2 cut(s) 442, 463
BstMAI GTCTC 4 cut(s) 55, 102, 394, 664
BstMBI GATC 2 cut(s) 439, 460
BstMWI GCNNNNNNNGC 1 cut(s) 417
BstNI CCWGG 2 cut(s) 482, 924
BstNSI RCATGY 1 cut(s) 589
BstSCI CCNGG 2 cut(s) 480, 922
BstV1I GCAGC 2 cut(s) 870, 1004
BstV2I GAAGAC 2 cut(s) 230, 942
BsuI GTATCC 1 cut(s) 554
BsuRI GGCC 1 cut(s) 508
BtgI CCRYGG 2 cut(s) 282, 724
BtsCI GGATG 3 cut(s) 274, 319, 810
BtsI GCAGTG 2 cut(s) 207, 513
BtsIMutI CAGTG 2 cut(s) 207, 513
BveI ACCTGC 1 cut(s) 14
Cac8I GCNNGC 4 cut(s) 99, 510, 583, 731
CaiI CAGNNNCTG 1 cut(s) 515
CciI TCATGA 1 cut(s) 376
CfoI GCGC 1 cut(s) 360
Cfr13I GGNCC 2 cut(s) 29, 599
CseI GACGC 1 cut(s) 587
Csp6I GTAC 5 cut(s) 352, 497, 710, 745, 888
CviAII CATG 5 cut(s) 85, 377, 586, 707, 934
CviQI GTAC 5 cut(s) 352, 497, 710, 745, 888
DdeI CTNAG 3 cut(s) 323, 395, 884
DpnI GATC 2 cut(s) 441, 462
DpnII GATC 2 cut(s) 439, 460
DrdI GACNNNNNNGTC 1 cut(s) 386
DseDI GACNNNNNNGTC 1 cut(s) 386
Eco130I CCWWGG 1 cut(s) 529
Eco24I GRGCYC 1 cut(s) 922
Eco31I GGTCTC 1 cut(s) 55
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 2 cut(s) 29, 599
EcoO109I RGGNCCY 1 cut(s) 599
EcoRII CCWGG 2 cut(s) 480, 922
EcoRV GATATC 1 cut(s) 610
EcoT14I CCWWGG 1 cut(s) 529
EcoT38I GRGCYC 1 cut(s) 922
ErhI CCWWGG 1 cut(s) 529
FaeI CATG 5 cut(s) 88, 380, 589, 710, 937
FalI AAGNNNNNCTT 2 cut(s) 587, 619
FaqI GGGAC 1 cut(s) 585
FatI CATG 5 cut(s) 84, 376, 585, 706, 933
FauI CCCGC 3 cut(s) 416, 590, 854
Fnu4HI GCNGC 2 cut(s) 859, 993
FokI GGATG 3 cut(s) 261, 326, 817
FriOI GRGCYC 1 cut(s) 922
Fsp4HI GCNGC 2 cut(s) 859, 993
FspBI CTAG 1 cut(s) 171
GlaI GCGC 1 cut(s) 359
GluI GCNGC 2 cut(s) 859, 993
GsaI CCCAGC 1 cut(s) 313
HaeIII GGCC 1 cut(s) 508
HapII CCGG 2 cut(s) 32, 974
HgaI GACGC 1 cut(s) 587
HhaI GCGC 1 cut(s) 360
Hin1I GRCGYC 1 cut(s) 579
Hin1II CATG 5 cut(s) 88, 380, 589, 710, 937
Hin6I GCGC 1 cut(s) 358
HinP1I GCGC 1 cut(s) 358
HincII GTYRAC 1 cut(s) 684
HindII GTYRAC 1 cut(s) 684
HinfI GANTC 7 cut(s) 73, 123, 182, 187, 317, 716, 970
HpaI GTTAAC 1 cut(s) 684
HpaII CCGG 2 cut(s) 32, 974
HphI GGTGA 1 cut(s) 839
Hpy166II GTNNAC 5 cut(s) 29, 207, 497, 684, 899
Hpy188I TCNGA 4 cut(s) 107, 444, 637, 955
Hpy188III TCNNGA 3 cut(s) 377, 437, 974
Hpy8I GTNNAC 5 cut(s) 29, 207, 497, 684, 899
Hpy99I CGWCG 1 cut(s) 301
HpyAV CCTTC 2 cut(s) 498, 1002
HpyCH4III ACNGT 2 cut(s) 286, 448
HpyCH4IV ACGT 3 cut(s) 215, 493, 743
HpyCH4V TGCA 3 cut(s) 212, 641, 941
HpyF10VI GCNNNNNNNGC 1 cut(s) 417
HpyF3I CTNAG 3 cut(s) 323, 395, 884
HpySE526I ACGT 3 cut(s) 215, 493, 743
Hsp92I GRCGYC 1 cut(s) 579
Hsp92II CATG 5 cut(s) 88, 380, 589, 710, 937
HspAI GCGC 1 cut(s) 358
KflI GGGWCCC 1 cut(s) 599
Kpn2I TCCGGA 1 cut(s) 973
KspAI GTTAAC 1 cut(s) 684
Kzo9I GATC 2 cut(s) 439, 460
LmnI GCTCC 3 cut(s) 58, 410, 925
Lsp1109I GCAGC 2 cut(s) 870, 1004
LweI GCATC 1 cut(s) 628
MaeI CTAG 1 cut(s) 171
MaeII ACGT 3 cut(s) 215, 493, 743
MaeIII GTNAC 2 cut(s) 163, 871
MalI GATC 2 cut(s) 441, 462
MboI GATC 2 cut(s) 439, 460
MboII GAAGA 3 cut(s) 53, 235, 942
MfeI CAATTG 1 cut(s) 546
MhlI GDGCHC 2 cut(s) 407, 922
MluCI AATT 1 cut(s) 546
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 5 cut(s) 61, 318, 775, 912, 941
MroI TCCGGA 1 cut(s) 973
MroXI GAANNNNTTC 1 cut(s) 127
MseI TTAA 3 cut(s) 231, 683, 912
MslI CAYNNNNRTG 2 cut(s) 569, 705
MspA1I CMGCKG 1 cut(s) 861
MspI CCGG 2 cut(s) 32, 974
MspR9I CCNGG 2 cut(s) 482, 924
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 9
MvaI CCWGG 2 cut(s) 482, 924
MwoI GCNNNNNNNGC 1 cut(s) 417
NdeII GATC 2 cut(s) 439, 460
NlaIII CATG 5 cut(s) 88, 380, 589, 710, 937
NlaIV GGNNCC 3 cut(s) 600, 601, 921
NmeAIII GCCGAG 1 cut(s) 496
NmuCI GTSAC 2 cut(s) 163, 871
NspI RCATGY 1 cut(s) 589
PagI TCATGA 1 cut(s) 376
PaqCI CACCTGC 1 cut(s) 14
PctI GAATGC 1 cut(s) 9
PdmI GAANNNNTTC 1 cut(s) 127
PfeI GAWTC 6 cut(s) 73, 123, 187, 317, 716, 970
PflMI CCANNNNNTGG 1 cut(s) 85
PfoI TCCNGGA 1 cut(s) 922
PkrI GCNGC 2 cut(s) 860, 994
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
Ppu21I YACGTR 2 cut(s) 494, 744
PpuMI RGGWCCY 1 cut(s) 599
Psp5II RGGWCCY 1 cut(s) 599
Psp6I CCWGG 2 cut(s) 480, 922
PspFI CCCAGC 1 cut(s) 309
PspGI CCWGG 2 cut(s) 480, 922
PspN4I GGNNCC 3 cut(s) 600, 601, 921
PspPI GGNCC 2 cut(s) 29, 599
PspPPI RGGWCCY 1 cut(s) 599
PsrI GAACNNNNNNTAC 2 cut(s) 112, 144
PstNI CAGNNNCTG 1 cut(s) 515
RsaI GTAC 5 cut(s) 353, 498, 711, 746, 889
RsaNI GTAC 5 cut(s) 352, 497, 710, 745, 888
RseI CAYNNNNRTG 2 cut(s) 569, 705
SaqAI TTAA 3 cut(s) 231, 683, 912
SatI GCNGC 2 cut(s) 859, 993
Sau3AI GATC 2 cut(s) 439, 460
Sau96I GGNCC 2 cut(s) 29, 599
ScaI AGTACT 2 cut(s) 353, 889
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 2 cut(s) 482, 924
SduI GDGCHC 2 cut(s) 407, 922
SfaNI GCATC 1 cut(s) 628
SinI GGWCC 2 cut(s) 29, 599
SmiMI CAYNNNNRTG 2 cut(s) 569, 705
Sse9I AATT 1 cut(s) 546
SsiI CCGC 3 cut(s) 409, 583, 861
SspMI CTAG 1 cut(s) 171
StyD4I CCNGG 2 cut(s) 480, 922
StyI CCWWGG 1 cut(s) 529
TaaI ACNGT 2 cut(s) 286, 448
TaiI ACGT 3 cut(s) 218, 496, 746
TaqI TCGA 3 cut(s) 152, 185, 299
TasI AATT 1 cut(s) 546
TatI WGTACW 4 cut(s) 351, 496, 709, 887
TfiI GAWTC 6 cut(s) 73, 123, 187, 317, 716, 970
Tru1I TTAA 3 cut(s) 231, 683, 912
Tru9I TTAA 3 cut(s) 231, 683, 912
TscAI CASTG 2 cut(s) 214, 520
TseFI GTSAC 2 cut(s) 163, 871
TseI GCWGC 2 cut(s) 858, 992
Tsp45I GTSAC 2 cut(s) 163, 871
TspDTI ATGAA 5 cut(s) 330, 412, 606, 695, 708
TspGWI ACGGA 2 cut(s) 80, 713
TspRI CASTG 2 cut(s) 214, 520
Van91I CCANNNNNTGG 1 cut(s) 85
VpaK11BI GGWCC 2 cut(s) 29, 599
XceI RCATGY 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 23
XmnI GAANNNNTTC 1 cut(s) 127
XspI CTAG 1 cut(s) 171
ZrmI AGTACT 2 cut(s) 353, 889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.