Rroxscaffold_3G00263950

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005287
Physical Location & Seq
Forward (+)
57749725 .. 57753387
3663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00263950.1

Sequence Viewer

Length: 603 bp
ATGGCGGGCGTTTTTTTGGCTCCCACAAGCACCACCCCAAAACTCGCTTGCAACCTCGCGTCCTTCTCCGAGTTCGGTTACTTCAAGGACCCTGCCGGAGATCGTGTATCGGCTTTTGGAGGGCTAGGACGTGAGGAAGAAACGAGAAGGCGGAGTGGAGGGAGAGGAAATGCACGGAGGCGGAAGCGACAGGAATCCGGATCCGACGCCGAAACCGGGTCGACCTCCGAGAAGAAGAAAGAGAGGAAAATGGAGAGAGAAGAAGACGATTCAATGAGCGAGTTATCCATGTACAATGTAAAGCTTGTCTCAAGCAATGCTACATTAGAGGCTTCTTTTAGCCCCGGATGGGATGTTTGTTATGTCGCTCAAAGAGAGAAGATAAGTGAAGCTAATCAAGTCTCTTTTGATATTGAAATGTTGACGTGTTGTTGTGGAGGAACCAAAAGATATCAGCTAGTATGCTTGCTCAATTTCTCTAGTGTGAAATTTCTTGCAACAAAATTGGGCCTTGGATTACAAGTTGTGAAATATTTGGCTGCCAAATATGAAGAGCTAGGGTGTGAAGTGAAATGGATGGGCAAGCCTGAAAAGGTGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

22.17

Weight (kDa)

8.63

Isoelectric Point (pI)

56.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 221
AccII CGCG 1 cut(s) 59
AccIII TCCGGA 1 cut(s) 197
AciI CCGC 3 cut(s) 5, 151, 181
AclWI GGATC 2 cut(s) 195, 208
AcsI RAATTY 1 cut(s) 488
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 1 cut(s) 293
AfiI CCNNNNNNNGG 2 cut(s) 216, 349
AflIII ACRYGT 1 cut(s) 425
AgsI TTSAA 3 cut(s) 85, 273, 416
AjiI CACGTC 2 cut(s) 131, 426
AluBI AGCT 4 cut(s) 304, 392, 457, 556
AluI AGCT 4 cut(s) 304, 392, 457, 556
Alw26I GTCTC 2 cut(s) 313, 406
AlwI GGATC 2 cut(s) 195, 208
Aor13HI TCCGGA 1 cut(s) 197
AoxI GGCC 1 cut(s) 508
ApeKI GCWGC 1 cut(s) 539
ApoI RAATTY 1 cut(s) 488
AspS9I GGNCC 2 cut(s) 88, 508
AsuC2I CCSGG 2 cut(s) 217, 345
AvaII GGWCC 1 cut(s) 88
BamHI GGATCC 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 270
BbvI GCAGC 1 cut(s) 526
BccI CCATC 2 cut(s) 342, 571
BcnI CCSGG 2 cut(s) 217, 345
BcoDI GTCTC 2 cut(s) 313, 406
BfaI CTAG 5 cut(s) 125, 458, 480, 557, 601
BisI GCNGC 1 cut(s) 540
BlsI GCNGC 1 cut(s) 541
Bme1390I CCNGG 2 cut(s) 217, 345
Bme18I GGWCC 1 cut(s) 88
BmgBI CACGTC 2 cut(s) 131, 426
BmgT120I GGNCC 2 cut(s) 88, 508
BmiI GGNNCC 4 cut(s) 21, 90, 202, 442
BmrFI CCNGG 2 cut(s) 217, 345
BpiI GAAGAC 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 295
BpuMI CCSGG 2 cut(s) 217, 345
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 343, 511
BsaWI WCCGGW 1 cut(s) 197
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 349
Bse3DI GCAATG 1 cut(s) 322
BseAI TCCGGA 1 cut(s) 197
BseDI CCNNGG 2 cut(s) 343, 511
BseGI GGATG 3 cut(s) 353, 358, 582
BseLI CCNNNNNNNGG 2 cut(s) 216, 349
BseMI GCAATG 1 cut(s) 322
BseXI GCAGC 1 cut(s) 526
Bsh1236I CGCG 1 cut(s) 59
BshFI GGCC 1 cut(s) 510
BsiSI CCGG 4 cut(s) 96, 198, 216, 345
BslI CCNNNNNNNGG 2 cut(s) 216, 349
BsmAI GTCTC 2 cut(s) 313, 406
BsnI GGCC 1 cut(s) 510
Bsp13I TCCGGA 1 cut(s) 197
Bsp1407I TGTACA 1 cut(s) 291
Bsp143I GATC 3 cut(s) 100, 200, 597
BspACI CCGC 3 cut(s) 5, 151, 181
BspANI GGCC 1 cut(s) 510
BspEI TCCGGA 1 cut(s) 197
BspFNI CGCG 1 cut(s) 59
BspLI GGNNCC 4 cut(s) 21, 90, 202, 442
BspPI GGATC 2 cut(s) 195, 208
BspQI GCTCTTC 1 cut(s) 546
BsrDI GCAATG 1 cut(s) 322
BsrGI TGTACA 1 cut(s) 291
BssECI CCNNGG 2 cut(s) 343, 511
BssMI GATC 3 cut(s) 100, 200, 597
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 511
Bst6I CTCTTC 1 cut(s) 546
BstACI GRCGYC 1 cut(s) 207
BstAUI TGTACA 1 cut(s) 291
BstC8I GCNNGC 4 cut(s) 7, 49, 467, 584
BstF5I GGATG 3 cut(s) 353, 358, 582
BstFNI CGCG 1 cut(s) 59
BstKTI GATC 3 cut(s) 103, 203, 600
BstMAI GTCTC 2 cut(s) 313, 406
BstMBI GATC 3 cut(s) 100, 200, 597
BstSCI CCNGG 2 cut(s) 215, 343
BstUI CGCG 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 526
BstV2I GAAGAC 1 cut(s) 270
BstX2I RGATCY 1 cut(s) 200
BstYI RGATCY 1 cut(s) 200
BsuRI GGCC 1 cut(s) 510
BtrI CACGTC 2 cut(s) 131, 426
BtsCI GGATG 3 cut(s) 353, 358, 582
Cac8I GCNNGC 4 cut(s) 7, 49, 467, 584
Cfr13I GGNCC 2 cut(s) 88, 508
CseI GACGC 2 cut(s) 48, 215
Csp6I GTAC 1 cut(s) 292
CviAII CATG 1 cut(s) 289
CviQI GTAC 1 cut(s) 292
DpnI GATC 3 cut(s) 102, 202, 599
DpnII GATC 3 cut(s) 100, 200, 597
Eam1104I CTCTTC 1 cut(s) 546
EarI CTCTTC 1 cut(s) 546
EciI GGCGGA 2 cut(s) 166, 196
Eco130I CCWWGG 1 cut(s) 511
Eco32I GATATC 1 cut(s) 452
Eco47I GGWCC 1 cut(s) 88
EcoO109I RGGNCCY 1 cut(s) 88
EcoRV GATATC 1 cut(s) 452
EcoT14I CCWWGG 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 511
FaeI CATG 1 cut(s) 292
FaiI YATR 4 cut(s) 290, 363, 463, 549
FatI CATG 1 cut(s) 288
FblI GTMKAC 1 cut(s) 221
Fnu4HI GCNGC 1 cut(s) 540
FokI GGATG 3 cut(s) 360, 365, 589
Fsp4HI GCNGC 1 cut(s) 540
FspBI CTAG 5 cut(s) 125, 458, 480, 557, 601
GluI GCNGC 1 cut(s) 540
HaeIII GGCC 1 cut(s) 510
HapII CCGG 4 cut(s) 96, 198, 216, 345
HgaI GACGC 2 cut(s) 48, 215
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 1 cut(s) 292
HincII GTYRAC 2 cut(s) 222, 423
HindII GTYRAC 2 cut(s) 222, 423
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 2 cut(s) 194, 269
HpaII CCGG 4 cut(s) 96, 198, 216, 345
Hpy166II GTNNAC 2 cut(s) 222, 423
Hpy188I TCNGA 3 cut(s) 70, 205, 229
Hpy188III TCNNGA 1 cut(s) 198
Hpy8I GTNNAC 2 cut(s) 222, 423
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 73, 141
HpyCH4IV ACGT 2 cut(s) 130, 425
HpyCH4V TGCA 3 cut(s) 51, 173, 497
HpySE526I ACGT 2 cut(s) 130, 425
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 1 cut(s) 292
Kpn2I TCCGGA 1 cut(s) 197
Kzo9I GATC 3 cut(s) 100, 200, 597
LguI GCTCTTC 1 cut(s) 546
LmnI GCTCC 1 cut(s) 25
LpnPI CCDG 6 cut(s) 105, 109, 176, 211, 229, 358
Lsp1109I GCAGC 1 cut(s) 526
MaeI CTAG 5 cut(s) 125, 458, 480, 557, 601
MaeII ACGT 2 cut(s) 130, 425
MaeIII GTNAC 1 cut(s) 77
MalI GATC 3 cut(s) 102, 202, 599
MboI GATC 3 cut(s) 100, 200, 597
MboII GAAGA 7 cut(s) 149, 244, 247, 272, 275, 391, 563
MflI RGATCY 1 cut(s) 200
MluCI AATT 3 cut(s) 472, 488, 503
MmeI TCCRAC 1 cut(s) 228
MroI TCCGGA 1 cut(s) 197
MspI CCGG 4 cut(s) 96, 198, 216, 345
MspR9I CCNGG 2 cut(s) 217, 345
MvnI CGCG 1 cut(s) 59
NciI CCSGG 2 cut(s) 217, 345
NdeII GATC 3 cut(s) 100, 200, 597
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 4 cut(s) 21, 90, 202, 442
PciSI GCTCTTC 1 cut(s) 546
PfeI GAWTC 2 cut(s) 194, 269
PkrI GCNGC 1 cut(s) 541
PpuMI RGGWCCY 1 cut(s) 88
Psp5II RGGWCCY 1 cut(s) 88
PspN4I GGNNCC 4 cut(s) 21, 90, 202, 442
PspPI GGNCC 2 cut(s) 88, 508
PspPPI RGGWCCY 1 cut(s) 88
PsuI RGATCY 1 cut(s) 200
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
SalI GTCGAC 1 cut(s) 220
SapI GCTCTTC 1 cut(s) 546
SatI GCNGC 1 cut(s) 540
Sau3AI GATC 3 cut(s) 100, 200, 597
Sau96I GGNCC 2 cut(s) 88, 508
ScrFI CCNGG 2 cut(s) 217, 345
SetI ASST 9 cut(s) 57, 133, 227, 306, 394, 428, 459, 558, 597
SinI GGWCC 1 cut(s) 88
SmlI CTYRAG 1 cut(s) 310
SmoI CTYRAG 1 cut(s) 310
Sse9I AATT 3 cut(s) 472, 488, 503
SsiI CCGC 3 cut(s) 5, 151, 181
SspI AATATT 1 cut(s) 533
SspMI CTAG 5 cut(s) 125, 458, 480, 557, 601
StyD4I CCNGG 2 cut(s) 215, 343
StyI CCWWGG 1 cut(s) 511
TaiI ACGT 2 cut(s) 133, 428
TaqI TCGA 1 cut(s) 221
TasI AATT 3 cut(s) 472, 488, 503
TatI WGTACW 1 cut(s) 291
TfiI GAWTC 2 cut(s) 194, 269
TseI GCWGC 1 cut(s) 539
TspDTI ATGAA 1 cut(s) 564
TspGWI ACGGA 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 88
XapI RAATTY 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 221
XspI CTAG 5 cut(s) 125, 458, 480, 557, 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.