Rw2G010020

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005287
Physical Location & Seq
Forward (+)
10166746 .. 10169465
2720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G010020.1

Sequence Viewer

Length: 882 bp
ATGTCAATGTCAAGCATTCCCCAAATGGCAGGTGGACCGGAAAGAGAAGATAAGGTCTCTTTGGAGCTGACGGAGGAGATTCTCCAAAGCATGGAAGTTGGGCTGGCTTTCAGAGACTACAATGGTAGAATCAGTTCGTTGGATTTTCACAAGGCTTCGAGTTATGTAGTGACTGCTAGTGATGATGAGTCGATTCGTTTGTATGATGTCTCCACTGCAACGTGCTTGAAGACCATTAACAGCAAAAAATATGGGGTTGATTTGGTCCGCTTTACTTCTCATCCTACCACGGTTATATACTCGTCGAAAAATGGCTGGGATGAATCGCTGAGGCTTCTCTCATTGAATGACAACAAGTACTTGCGCTATTTCAAAGGACATCATGACAGGGTTGTCTCACTTAGCTTGTGCTCCCGCAAAGAGTGCTTCATTTCTGGTTCTCTTGATCGGACAGTTCTGCTTTGGGATCAACGAGCCGAGAAGTGCCAGGGTCTTTTACGTGTACAAGGAAGGCCTGCCACTGCTTATGATGACCAAGGGCTTGTTTTTACAATTGCCTTTGGTGGATACATACGAATGTTTGATGCCCGCATGTATGAAAAGGGTCCCTTTGATATCTTTTCTGTTGGGGGAGATATGTCTGATGCAAATGTTGTAAAGTTCAGTAATGATGGGAGACTTATGCTGTTAACAACGACAGATGGACATATTCATGTACTTGATTCATTCCGTGGCACGCTTTTATCCACGTACAATGTAAAGCCCGTTTCAAGCAATGCTACATTAGAGGCTTCTTTTAGCCCAGATGGGATGTTTGTTATGTCAGGTTCAGGTGATGGTAGTGTCTATGCTTGGAGTGTTCGGAGCGGGAAAGAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
WD40

Protein Analysis

293

Amino Acids

32.55

Weight (kDa)

5.89

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 27 - 159 7.4e-06 TEP-1 second beta-propeller
WD40_Prp19 PF24814 35 - 234 2.6e-17 Prp19 WD40 domain
EIF3I PF24805 37 - 105 2.2e-06 EIF3I
Beta-prop_WDR3_1st PF25173 38 - 103 1.8e-07 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 38 - 161 7.7e-12 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 40 - 102 8.6e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 41 - 290 2.7e-37 WDR5 beta-propeller domain
WDR55 PF24796 43 - 162 2.7e-08 WDR55
Beta-prop_WDR3_2nd PF25172 44 - 160 7.6e-11 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 46 - 155 7.4e-06 MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 52 - 170 1e-05 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 88 - 250 1.3e-10 WDHD1 first WD40 domain
NBCH_WD40 PF20426 105 - 159 2.3e-06 Neurobeachin beta propeller domain
Beta-prop_THOC3 PF25174 106 - 291 8.3e-18 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 107 - 170 8e-07 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 121 - 285 8e-09 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 134 - 292 3.5e-11 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 20
AasI GACNNNNNNGTC 1 cut(s) 392
Acc36I ACCTGC 1 cut(s) 20
AccB7I CCANNNNNTGG 1 cut(s) 91
AccBSI CCGCTC 1 cut(s) 867
AciI CCGC 4 cut(s) 268, 415, 589, 867
AclWI GGATC 1 cut(s) 474
AfaI GTAC 4 cut(s) 359, 504, 717, 752
AfiI CCNNNNNNNGG 1 cut(s) 91
AflIII ACRYGT 1 cut(s) 499
AgsI TTSAA 4 cut(s) 229, 346, 373, 771
AjnI CCWGG 1 cut(s) 486
AluBI AGCT 2 cut(s) 67, 405
AluI AGCT 2 cut(s) 67, 405
Alw21I GWGCWC 1 cut(s) 413
Alw26I GTCTC 5 cut(s) 61, 108, 214, 400, 670
AlwI GGATC 1 cut(s) 474
AoxI GGCC 1 cut(s) 512
Asp700I GAANNNNTTC 1 cut(s) 133
AspLEI GCGC 1 cut(s) 366
AspS9I GGNCC 3 cut(s) 35, 265, 605
AsuHPI GGTGA 1 cut(s) 845
AvaII GGWCC 3 cut(s) 35, 265, 605
BbsI GAAGAC 1 cut(s) 236
Bbv12I GWGCWC 1 cut(s) 413
BbvCI CCTCAGC 1 cut(s) 329
BccI CCATC 4 cut(s) 665, 695, 800, 830
BciT130I CCWGG 1 cut(s) 488
BciVI GTATCC 1 cut(s) 560
BcoDI GTCTC 5 cut(s) 61, 108, 214, 400, 670
BfaI CTAG 1 cut(s) 177
BfuAI ACCTGC 1 cut(s) 20
BfuI GTATCC 1 cut(s) 560
BmcAI AGTACT 1 cut(s) 359
Bme1390I CCNGG 1 cut(s) 488
Bme18I GGWCC 3 cut(s) 35, 265, 605
BmgT120I GGNCC 3 cut(s) 35, 265, 605
BmiI GGNNCC 2 cut(s) 606, 607
BmrFI CCNGG 1 cut(s) 488
BmsI GCATC 2 cut(s) 574, 634
BpiI GAAGAC 1 cut(s) 236
Bpu10I CCTNAGC 1 cut(s) 329
BsaAI YACGTR 2 cut(s) 500, 750
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 4 cut(s) 288, 487, 535, 730
BsaWI WCCGGW 1 cut(s) 37
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse3DI GCAATG 1 cut(s) 781
BseBI CCWGG 1 cut(s) 488
BseDI CCNNGG 4 cut(s) 288, 487, 535, 730
BseGI GGATG 3 cut(s) 280, 325, 816
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMI GCAATG 1 cut(s) 781
BseMII CTCAG 1 cut(s) 320
BseRI GAGGAG 1 cut(s) 89
BseYI CCCAGC 1 cut(s) 315
BshFI GGCC 1 cut(s) 514
BsiHKAI GWGCWC 1 cut(s) 413
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 1 cut(s) 591
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 5 cut(s) 61, 108, 214, 400, 670
BsmFI GGGAC 1 cut(s) 591
BsmI GAATGC 1 cut(s) 15
BsnI GGCC 1 cut(s) 514
Bso31I GGTCTC 1 cut(s) 61
Bsp1286I GDGCHC 1 cut(s) 413
Bsp1407I TGTACA 1 cut(s) 502
Bsp143I GATC 2 cut(s) 445, 466
BspACI CCGC 4 cut(s) 268, 415, 589, 867
BspANI GGCC 1 cut(s) 514
BspCNI CTCAG 1 cut(s) 321
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 2 cut(s) 606, 607
BspMI ACCTGC 1 cut(s) 20
BspPI GGATC 1 cut(s) 474
BspTNI GGTCTC 1 cut(s) 61
BsrBI CCGCTC 1 cut(s) 867
BsrDI GCAATG 1 cut(s) 781
BsrGI TGTACA 1 cut(s) 502
BssECI CCNNGG 4 cut(s) 288, 487, 535, 730
BssMI GATC 2 cut(s) 445, 466
BssT1I CCWWGG 1 cut(s) 535
Bst2UI CCWGG 1 cut(s) 488
Bst4CI ACNGT 2 cut(s) 292, 454
BstAPI GCANNNNNTGC 1 cut(s) 423
BstAUI TGTACA 1 cut(s) 502
BstBAI YACGTR 2 cut(s) 500, 750
BstC8I GCNNGC 4 cut(s) 105, 516, 589, 737
BstDEI CTNAG 2 cut(s) 329, 401
BstDSI CCRYGG 2 cut(s) 288, 730
BstF5I GGATG 3 cut(s) 280, 325, 816
BstHHI GCGC 1 cut(s) 366
BstKTI GATC 2 cut(s) 448, 469
BstMAI GTCTC 5 cut(s) 61, 108, 214, 400, 670
BstMBI GATC 2 cut(s) 445, 466
BstMWI GCNNNNNNNGC 1 cut(s) 423
BstNI CCWGG 1 cut(s) 488
BstNSI RCATGY 1 cut(s) 595
BstSCI CCNGG 1 cut(s) 486
BstV2I GAAGAC 1 cut(s) 236
BsuI GTATCC 1 cut(s) 560
BsuRI GGCC 1 cut(s) 514
BtgI CCRYGG 2 cut(s) 288, 730
BtsCI GGATG 3 cut(s) 280, 325, 816
BtsI GCAGTG 2 cut(s) 213, 519
BtsIMutI CAGTG 2 cut(s) 213, 519
BveI ACCTGC 1 cut(s) 20
Cac8I GCNNGC 4 cut(s) 105, 516, 589, 737
CciI TCATGA 1 cut(s) 382
CfoI GCGC 1 cut(s) 366
Cfr13I GGNCC 3 cut(s) 35, 265, 605
Csp6I GTAC 4 cut(s) 358, 503, 716, 751
CviAII CATG 4 cut(s) 91, 383, 592, 713
CviQI GTAC 4 cut(s) 358, 503, 716, 751
DdeI CTNAG 2 cut(s) 329, 401
DpnI GATC 2 cut(s) 447, 468
DpnII GATC 2 cut(s) 445, 466
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
Eco130I CCWWGG 1 cut(s) 535
Eco147I AGGCCT 1 cut(s) 514
Eco31I GGTCTC 1 cut(s) 61
Eco32I GATATC 1 cut(s) 616
Eco47I GGWCC 3 cut(s) 35, 265, 605
EcoO109I RGGNCCY 1 cut(s) 605
EcoRII CCWGG 1 cut(s) 486
EcoRV GATATC 1 cut(s) 616
EcoT14I CCWWGG 1 cut(s) 535
ErhI CCWWGG 1 cut(s) 535
FaeI CATG 4 cut(s) 94, 386, 595, 716
FalI AAGNNNNNCTT 2 cut(s) 593, 625
FaqI GGGAC 1 cut(s) 591
FatI CATG 4 cut(s) 90, 382, 591, 712
FauI CCCGC 3 cut(s) 422, 596, 860
FokI GGATG 3 cut(s) 267, 332, 823
FspBI CTAG 1 cut(s) 177
GlaI GCGC 1 cut(s) 365
GsaI CCCAGC 1 cut(s) 319
HaeIII GGCC 1 cut(s) 514
HapII CCGG 1 cut(s) 38
HhaI GCGC 1 cut(s) 366
Hin1II CATG 4 cut(s) 94, 386, 595, 716
Hin6I GCGC 1 cut(s) 364
HinP1I GCGC 1 cut(s) 364
HincII GTYRAC 1 cut(s) 690
HindII GTYRAC 1 cut(s) 690
HinfI GANTC 6 cut(s) 79, 129, 188, 193, 323, 722
HpaI GTTAAC 1 cut(s) 690
HpaII CCGG 1 cut(s) 38
HphI GGTGA 1 cut(s) 845
Hpy166II GTNNAC 3 cut(s) 35, 503, 690
Hpy188I TCNGA 4 cut(s) 113, 450, 643, 864
Hpy188III TCNNGA 2 cut(s) 383, 443
Hpy8I GTNNAC 3 cut(s) 35, 503, 690
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 1 cut(s) 504
HpyCH4III ACNGT 2 cut(s) 292, 454
HpyCH4IV ACGT 3 cut(s) 221, 499, 749
HpyCH4V TGCA 2 cut(s) 218, 647
HpyF10VI GCNNNNNNNGC 1 cut(s) 423
HpyF3I CTNAG 2 cut(s) 329, 401
HpySE526I ACGT 3 cut(s) 221, 499, 749
Hsp92II CATG 4 cut(s) 94, 386, 595, 716
HspAI GCGC 1 cut(s) 364
KflI GGGWCCC 1 cut(s) 605
KspAI GTTAAC 1 cut(s) 690
Kzo9I GATC 2 cut(s) 445, 466
LmnI GCTCC 3 cut(s) 64, 416, 864
LweI GCATC 2 cut(s) 574, 634
MaeI CTAG 1 cut(s) 177
MaeII ACGT 3 cut(s) 221, 499, 749
MaeIII GTNAC 1 cut(s) 169
MalI GATC 2 cut(s) 447, 468
MbiI CCGCTC 1 cut(s) 867
MboI GATC 2 cut(s) 445, 466
MboII GAAGA 2 cut(s) 59, 241
MfeI CAATTG 1 cut(s) 552
MhlI GDGCHC 1 cut(s) 413
MluCI AATT 1 cut(s) 552
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 3 cut(s) 67, 324, 781
MroXI GAANNNNTTC 1 cut(s) 133
MseI TTAA 2 cut(s) 237, 689
MslI CAYNNNNRTG 2 cut(s) 575, 711
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 488
MunI CAATTG 1 cut(s) 552
Mva1269I GAATGC 1 cut(s) 15
MvaI CCWGG 1 cut(s) 488
MwoI GCNNNNNNNGC 1 cut(s) 423
NdeII GATC 2 cut(s) 445, 466
NlaIII CATG 4 cut(s) 94, 386, 595, 716
NlaIV GGNNCC 2 cut(s) 606, 607
NmeAIII GCCGAG 1 cut(s) 502
NmuCI GTSAC 1 cut(s) 169
NspI RCATGY 1 cut(s) 595
PagI TCATGA 1 cut(s) 382
PaqCI CACCTGC 1 cut(s) 20
PceI AGGCCT 1 cut(s) 514
PctI GAATGC 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 133
PfeI GAWTC 5 cut(s) 79, 129, 193, 323, 722
PflMI CCANNNNNTGG 1 cut(s) 91
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
Ppu21I YACGTR 2 cut(s) 500, 750
PpuMI RGGWCCY 1 cut(s) 605
Psp5II RGGWCCY 1 cut(s) 605
Psp6I CCWGG 1 cut(s) 486
PspFI CCCAGC 1 cut(s) 315
PspGI CCWGG 1 cut(s) 486
PspN4I GGNNCC 2 cut(s) 606, 607
PspPI GGNCC 3 cut(s) 35, 265, 605
PspPPI RGGWCCY 1 cut(s) 605
PsrI GAACNNNNNNTAC 2 cut(s) 118, 150
RsaI GTAC 4 cut(s) 359, 504, 717, 752
RsaNI GTAC 4 cut(s) 358, 503, 716, 751
RseI CAYNNNNRTG 2 cut(s) 575, 711
SaqAI TTAA 2 cut(s) 237, 689
Sau3AI GATC 2 cut(s) 445, 466
Sau96I GGNCC 3 cut(s) 35, 265, 605
ScaI AGTACT 1 cut(s) 359
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 488
SduI GDGCHC 1 cut(s) 413
SetI ASST 9 cut(s) 34, 57, 69, 224, 407, 502, 752, 829, 835
SfaNI GCATC 2 cut(s) 574, 634
SinI GGWCC 3 cut(s) 35, 265, 605
SmiMI CAYNNNNRTG 2 cut(s) 575, 711
Sse9I AATT 1 cut(s) 552
SseBI AGGCCT 1 cut(s) 514
SsiI CCGC 4 cut(s) 268, 415, 589, 867
SspMI CTAG 1 cut(s) 177
StuI AGGCCT 1 cut(s) 514
StyD4I CCNGG 1 cut(s) 486
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 2 cut(s) 292, 454
TaiI ACGT 3 cut(s) 224, 502, 752
TaqI TCGA 3 cut(s) 158, 191, 305
TasI AATT 1 cut(s) 552
TatI WGTACW 3 cut(s) 357, 502, 715
TfiI GAWTC 5 cut(s) 79, 129, 193, 323, 722
Tru1I TTAA 2 cut(s) 237, 689
Tru9I TTAA 2 cut(s) 237, 689
TscAI CASTG 2 cut(s) 220, 526
TseFI GTSAC 1 cut(s) 169
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 5 cut(s) 336, 418, 612, 701, 714
TspGWI ACGGA 2 cut(s) 86, 719
TspRI CASTG 2 cut(s) 220, 526
Van91I CCANNNNNTGG 1 cut(s) 91
VpaK11BI GGWCC 3 cut(s) 35, 265, 605
XceI RCATGY 1 cut(s) 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 29
XmnI GAANNNNTTC 1 cut(s) 133
XspI CTAG 1 cut(s) 177
ZrmI AGTACT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.