MD15G1245700.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005287
Physical Location & Seq
Reverse (-)
20295423 .. 20297414
1992 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1245700.v1.1.491

Sequence Viewer

Length: 816 bp
ATGGAAGTTGGTTTAGCTTTTAGAGACTATAGTGGTAGAATTAGTTCATTGGACTTCCACAAGGCGTGTAGTTATGTAGTGACTGCTAGTGATGACGAGTCGATTCGCCTGTATGATGTCTCTAGTGCAACATGCTTGAAGACCATCAACAGTAAAAAGTATGGAGTTGGTTTAGTTTGCTTTACTTCTCATCCGACAACTGTTATATACTCTTCCAAAAATGGGTGGGATGAATCCTTGAGGCTTCTCTCGTTGAATGACAATATGTACTTGCGCTACTTCAAAGGCCATCATGACAGGGTATTCTCACTTAGCTTGTGTTCTCGCAAAGACTGCTTCATCTCTGGTTCTCTTGATAGAACTGTACTGCTATGGGATCAACGGGCAGAGAAGTGTCAGGGTCCTTTTGATATGTTTTCTGTTGGGGGAGATATGTCGGATGCAAATGTTGTAAAGTTCAGTAACGATGGGAGGCTTATGCTTTCAACAACCACAGGTGGACATATTCATGTCCTTGATTCATTCCGTGGAACACTATTATCCACATACAATGTTAAGCCAGTCTCCAGCAATTCAACATTAGAGGCTTCTTTTAGCCCCGAGGGGATGTTTGTCATGTCAGGTTCAGGTGATGGTAGTGTCTATGCTTGGGTGGTTCGGAGCGGGAAAGAAGTCACAAGCTGGAAGAGCACCGAAAATGAACCTCCAGTTATCAAATGGGCTCCCGGAAACCTCATGTTTGCAACTGGATCGTCCGAACTACCATTTTGGATTCCAGATTTGTCTAAGTTGGGATCTCACGTTGGAAGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
WD40

Protein Analysis

272

Amino Acids

29.92

Weight (kDa)

7.04

Isoelectric Point (pI)

32.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 7 - 74 1e-06 THOC3 beta-propeller domain
WD40_Prp19 PF24814 7 - 69 1.7e-07 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 9 - 73 1.1e-06 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 10 - 252 2.8e-34 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 11 - 131 5.7e-11 CDC20/Fizzy WD40 domain
WDR55 PF24796 13 - 132 1.9e-06 WDR55
Beta-prop_WDR3_2nd PF25172 18 - 128 4.4e-10 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 60 - 242 9.3e-16 WDHD1 first WD40 domain
NBCH_WD40 PF20426 74 - 129 9.7e-06 Neurobeachin beta propeller domain
WD40_Prp19 PF24814 75 - 253 5.5e-14 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 77 - 252 8.4e-19 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 77 - 213 3e-08 WDR3 first beta-propeller domain
WD40 PF00400 91 - 126 7.1e-06 WD domain, G-beta repeat
WD40_Gbeta PF25391 91 - 164 3e-06 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 103 - 228 2.5e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 146 - 254 3.6e-06 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 663
AciI CCGC 1 cut(s) 663
AclWI GGATC 3 cut(s) 384, 757, 802
AfaI GTAC 2 cut(s) 269, 366
AfiI CCNNNNNNNGG 1 cut(s) 222
AgsI TTSAA 5 cut(s) 139, 256, 283, 486, 576
AluBI AGCT 3 cut(s) 17, 315, 681
AluI AGCT 3 cut(s) 17, 315, 681
Alw21I GWGCWC 1 cut(s) 692
Alw26I GTCTC 3 cut(s) 18, 124, 568
AlwI GGATC 3 cut(s) 384, 757, 802
Ama87I CYCGRG 1 cut(s) 599
AoxI GGCC 1 cut(s) 286
Asp700I GAANNNNTTC 1 cut(s) 43
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 401
AsuC2I CCSGG 1 cut(s) 726
AsuHPI GGTGA 1 cut(s) 641
AvaI CYCGRG 1 cut(s) 599
AvaII GGWCC 1 cut(s) 401
BanII GRGCYC 1 cut(s) 724
BbsI GAAGAC 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 692
BccI CCATC 4 cut(s) 152, 297, 461, 626
BcgI CGANNNNNNTGC 2 cut(s) 732, 766
BcnI CCSGG 1 cut(s) 726
BcoDI GTCTC 3 cut(s) 18, 124, 568
BfaI CTAG 2 cut(s) 87, 123
BfmI CTRYAG 1 cut(s) 28
Bme1390I CCNGG 1 cut(s) 726
Bme18I GGWCC 1 cut(s) 401
BmeT110I CYCGRG 1 cut(s) 599
BmgT120I GGNCC 1 cut(s) 401
BmiI GGNNCC 2 cut(s) 402, 723
BmrFI CCNGG 1 cut(s) 726
BmsI GCATC 1 cut(s) 430
BpiI GAAGAC 1 cut(s) 146
BpmI CTGGAG 2 cut(s) 550, 690
BpuEI CTTGAG 1 cut(s) 259
BpuMI CCSGG 1 cut(s) 726
BsaJI CCNNGG 2 cut(s) 526, 600
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse1I ACTGG 3 cut(s) 560, 707, 751
BseDI CCNNGG 2 cut(s) 526, 600
BseGI GGATG 4 cut(s) 190, 235, 445, 612
BseLI CCNNNNNNNGG 1 cut(s) 222
BseNI ACTGG 3 cut(s) 560, 707, 751
BshFI GGCC 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 692
BsiHKCI CYCGRG 1 cut(s) 599
BsiSI CCGG 1 cut(s) 726
BslI CCNNNNNNNGG 1 cut(s) 222
BsmAI GTCTC 3 cut(s) 18, 124, 568
BsnI GGCC 1 cut(s) 288
BsoBI CYCGRG 1 cut(s) 599
Bsp1286I GDGCHC 2 cut(s) 692, 724
Bsp143I GATC 3 cut(s) 376, 749, 794
BspACI CCGC 1 cut(s) 663
BspANI GGCC 1 cut(s) 288
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 2 cut(s) 402, 723
BspPI GGATC 3 cut(s) 384, 757, 802
BspQI GCTCTTC 1 cut(s) 680
BsrBI CCGCTC 1 cut(s) 663
BsrI ACTGG 3 cut(s) 560, 707, 751
BssECI CCNNGG 2 cut(s) 526, 600
BssMI GATC 3 cut(s) 376, 749, 794
Bst4CI ACNGT 3 cut(s) 152, 202, 364
Bst6I CTCTTC 2 cut(s) 217, 680
BstAPI GCANNNNNTGC 1 cut(s) 333
BstDEI CTNAG 2 cut(s) 311, 786
BstDSI CCRYGG 1 cut(s) 526
BstF5I GGATG 4 cut(s) 190, 235, 445, 612
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 3 cut(s) 379, 752, 797
BstMAI GTCTC 3 cut(s) 18, 124, 568
BstMBI GATC 3 cut(s) 376, 749, 794
BstMWI GCNNNNNNNGC 2 cut(s) 333, 687
BstNSI RCATGY 1 cut(s) 135
BstSCI CCNGG 1 cut(s) 724
BstSFI CTRYAG 1 cut(s) 28
BstV2I GAAGAC 1 cut(s) 146
BstX2I RGATCY 1 cut(s) 794
BstYI RGATCY 1 cut(s) 794
BsuRI GGCC 1 cut(s) 288
BtgI CCRYGG 1 cut(s) 526
BtsCI GGATG 4 cut(s) 190, 235, 445, 612
CciI TCATGA 1 cut(s) 292
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 1 cut(s) 401
Csp6I GTAC 2 cut(s) 268, 365
CviAII CATG 5 cut(s) 132, 293, 509, 616, 736
CviQI GTAC 2 cut(s) 268, 365
DdeI CTNAG 2 cut(s) 311, 786
DpnI GATC 3 cut(s) 378, 751, 796
DpnII GATC 3 cut(s) 376, 749, 794
Eam1104I CTCTTC 2 cut(s) 217, 680
EarI CTCTTC 2 cut(s) 217, 680
Eco24I GRGCYC 1 cut(s) 724
Eco47I GGWCC 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 599
EcoO109I RGGNCCY 1 cut(s) 401
EcoT38I GRGCYC 1 cut(s) 724
FaeI CATG 5 cut(s) 135, 296, 512, 619, 739
FatI CATG 5 cut(s) 131, 292, 508, 615, 735
FauI CCCGC 1 cut(s) 656
FokI GGATG 4 cut(s) 177, 242, 452, 619
FriOI GRGCYC 1 cut(s) 724
FspBI CTAG 2 cut(s) 87, 123
GlaI GCGC 1 cut(s) 275
GsuI CTGGAG 2 cut(s) 550, 690
HaeIII GGCC 1 cut(s) 288
HapII CCGG 1 cut(s) 726
HhaI GCGC 1 cut(s) 276
Hin1II CATG 5 cut(s) 135, 296, 512, 619, 739
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HinfI GANTC 5 cut(s) 98, 103, 233, 518, 772
HpaII CCGG 1 cut(s) 726
HphI GGTGA 1 cut(s) 641
Hpy166II GTNNAC 1 cut(s) 500
Hpy188I TCNGA 4 cut(s) 195, 439, 660, 757
Hpy188III TCNNGA 3 cut(s) 293, 353, 776
Hpy8I GTNNAC 1 cut(s) 500
HpyCH4III ACNGT 3 cut(s) 152, 202, 364
HpyCH4IV ACGT 1 cut(s) 801
HpyCH4V TGCA 3 cut(s) 128, 443, 743
HpyF10VI GCNNNNNNNGC 2 cut(s) 333, 687
HpyF3I CTNAG 2 cut(s) 311, 786
HpySE526I ACGT 1 cut(s) 801
Hsp92II CATG 5 cut(s) 135, 296, 512, 619, 739
HspAI GCGC 1 cut(s) 274
Kzo9I GATC 3 cut(s) 376, 749, 794
LguI GCTCTTC 1 cut(s) 680
LmnI GCTCC 2 cut(s) 660, 727
LweI GCATC 1 cut(s) 430
MaeI CTAG 2 cut(s) 87, 123
MaeII ACGT 1 cut(s) 801
MaeIII GTNAC 3 cut(s) 79, 461, 673
MalI GATC 3 cut(s) 378, 751, 796
MbiI CCGCTC 1 cut(s) 663
MboI GATC 3 cut(s) 376, 749, 794
MboII GAAGA 3 cut(s) 151, 204, 697
MflI RGATCY 1 cut(s) 794
MhlI GDGCHC 2 cut(s) 692, 724
MluCI AATT 2 cut(s) 39, 571
MlyI GAGTC 1 cut(s) 107
MmeI TCCRAC 3 cut(s) 218, 417, 784
MnlI CCTC 6 cut(s) 234, 465, 577, 595, 714, 743
MroXI GAANNNNTTC 1 cut(s) 43
MseI TTAA 1 cut(s) 555
MslI CAYNNNNRTG 1 cut(s) 507
MspI CCGG 1 cut(s) 726
MspR9I CCNGG 1 cut(s) 726
MwoI GCNNNNNNNGC 2 cut(s) 333, 687
NciI CCSGG 1 cut(s) 726
NdeII GATC 3 cut(s) 376, 749, 794
NlaIII CATG 5 cut(s) 135, 296, 512, 619, 739
NlaIV GGNNCC 2 cut(s) 402, 723
NmuCI GTSAC 2 cut(s) 79, 673
NspI RCATGY 1 cut(s) 135
PagI TCATGA 1 cut(s) 292
PciSI GCTCTTC 1 cut(s) 680
PdmI GAANNNNTTC 1 cut(s) 43
PfeI GAWTC 4 cut(s) 103, 233, 518, 772
PfoI TCCNGGA 1 cut(s) 724
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PpuMI RGGWCCY 1 cut(s) 401
Psp5II RGGWCCY 1 cut(s) 401
PspN4I GGNNCC 2 cut(s) 402, 723
PspPI GGNCC 1 cut(s) 401
PspPPI RGGWCCY 1 cut(s) 401
PsrI GAACNNNNNNTAC 2 cut(s) 28, 60
PsuI RGATCY 1 cut(s) 794
RsaI GTAC 2 cut(s) 269, 366
RsaNI GTAC 2 cut(s) 268, 365
RseI CAYNNNNRTG 1 cut(s) 507
SapI GCTCTTC 1 cut(s) 680
SaqAI TTAA 1 cut(s) 555
Sau3AI GATC 3 cut(s) 376, 749, 794
Sau96I GGNCC 1 cut(s) 401
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 1 cut(s) 726
SduI GDGCHC 2 cut(s) 692, 724
SetI ASST 9 cut(s) 19, 317, 499, 625, 631, 683, 706, 735, 804
SfaNI GCATC 1 cut(s) 430
SfcI CTRYAG 1 cut(s) 28
SinI GGWCC 1 cut(s) 401
SmiMI CAYNNNNRTG 1 cut(s) 507
SmlI CTYRAG 1 cut(s) 238
SmoI CTYRAG 1 cut(s) 238
Sse9I AATT 2 cut(s) 39, 571
SsiI CCGC 1 cut(s) 663
SspMI CTAG 2 cut(s) 87, 123
StyD4I CCNGG 1 cut(s) 724
TaaI ACNGT 3 cut(s) 152, 202, 364
TaiI ACGT 1 cut(s) 804
TaqI TCGA 1 cut(s) 101
TasI AATT 2 cut(s) 39, 571
TatI WGTACW 2 cut(s) 267, 364
TfiI GAWTC 4 cut(s) 103, 233, 518, 772
Tru1I TTAA 1 cut(s) 555
Tru9I TTAA 1 cut(s) 555
TseFI GTSAC 2 cut(s) 79, 673
Tsp45I GTSAC 2 cut(s) 79, 673
TspDTI ATGAA 6 cut(s) 36, 246, 328, 497, 510, 714
TspGWI ACGGA 1 cut(s) 515
VpaK11BI GGWCC 1 cut(s) 401
XceI RCATGY 1 cut(s) 135
XcmI CCANNNNNNNNNTGG 1 cut(s) 714
XmnI GAANNNNTTC 1 cut(s) 43
XspI CTAG 2 cut(s) 87, 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.