AT5G66440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009081
Physical Location & Seq
Reverse (-)
26529919 .. 26531176
1258 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66440.1

Sequence Viewer

Length: 759 bp
ATGGATTCGTTTGATTTCGATAACGTGAAAGCAGAGAAAGCCAAGGCGTTGCGGCGTTTCAACCGCTTCTGTAGAATTGGCCGTTTCTTCCGTGCGGCGGAGGTTTGCGTCGCTCTACTCTTCATCTTTTGGACATTTTCGCAACTTCCTTTCGTCGTTCAAATCTCCGGAGCGTTTCTCCGCCGTATCGCCGCCGTCATATCTACTCCTCTGTTCGTTTTCCTCCTCGGGAACTCCATCGTCGTTGTTCTTCTCACCAAGTCTTCCGATCAGACAACCACCGCCGTCGCAAGCGCAGAAACCGAAATCTACCAGGCGTTCGTCAGATCTGTAGAGAATCGTTCCAAACCGTCCGATGAAGATTTGACGGAGGGGATTGTCTACGACGACAAACAGGTGATCGTTACTGATTTGGATTCAAATCCGAATCTAACGGTGGATGAGAATATGCCTCACGTCGAGATTGACTCAGATTCGAGTCCCCATAGTAAGGTCTATGGAAGAAGCAAATCAGACGTGTCTGGAAAACAGAGTTCAAACATGGTGATGATGATGATGAAACGTTCTCTTCAGAGATCGGAGACAGAGAAGTTCTGCAAAGAAACTGAGAACGATGATGACTCTGAGGAGGAGAAGAACTATCCAGAGGATGATCTGAGCAACGAAGAGTTTCAGAAAACGATAGAAGCTTTCATAGCAAAACAGAGGATGTTTCGTCGTCAAGAATCTTTAGCCGTCGTTGTCCATAACATAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408

Protein Analysis

252

Amino Acids

28.72

Weight (kDa)

5.02

Isoelectric Point (pI)

61.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 381
AccIII TCCGGA 1 cut(s) 167
AciI CCGC 7 cut(s) 52, 64, 95, 98, 181, 192, 282
AclI AACGTT 1 cut(s) 562
AcoI YGGCCR 1 cut(s) 79
AcuI CTGAAG 1 cut(s) 554
AfiI CCNNNNNNNGG 2 cut(s) 97, 490
AflIII ACRYGT 1 cut(s) 516
AgsI TTSAA 4 cut(s) 61, 161, 420, 537
AjiI CACGTC 2 cut(s) 457, 517
AjnI CCWGG 1 cut(s) 312
AluBI AGCT 1 cut(s) 689
AluI AGCT 1 cut(s) 689
Alw26I GTCTC 1 cut(s) 575
Ama87I CYCGRG 1 cut(s) 227
Aor13HI TCCGGA 1 cut(s) 167
AoxI GGCC 1 cut(s) 79
Asp700I GAANNNNTTC 1 cut(s) 669
AspLEI GCGC 1 cut(s) 296
AsuHPI GGTGA 3 cut(s) 247, 409, 556
AvaI CYCGRG 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 255
BccI CCATC 1 cut(s) 245
BceAI ACGGC 5 cut(s) 66, 168, 179, 269, 719
BciT130I CCWGG 1 cut(s) 314
BcoDI GTCTC 1 cut(s) 575
BfmI CTRYAG 2 cut(s) 70, 330
BglII AGATCT 1 cut(s) 326
BisI GCNGC 3 cut(s) 53, 96, 192
BlsI GCNGC 3 cut(s) 54, 97, 193
Bme1390I CCNGG 1 cut(s) 314
BmeT110I CYCGRG 1 cut(s) 227
BmgBI CACGTC 2 cut(s) 457, 517
BmrFI CCNGG 1 cut(s) 314
BpiI GAAGAC 1 cut(s) 255
BplI GAGNNNNNCTC 4 cut(s) 162, 194, 452, 484
BsaBI GATNNNNATC 1 cut(s) 420
BsaJI CCNNGG 2 cut(s) 42, 226
BsaWI WCCGGW 1 cut(s) 167
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 490
Bse8I GATNNNNATC 1 cut(s) 420
BseAI TCCGGA 1 cut(s) 167
BseBI CCWGG 1 cut(s) 314
BseDI CCNNGG 2 cut(s) 42, 226
BseGI GGATG 3 cut(s) 445, 655, 714
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 2 cut(s) 97, 490
BseMII CTCAG 4 cut(s) 483, 597, 615, 647
BseRI GAGGAG 4 cut(s) 198, 215, 641, 644
BshFI GGCC 1 cut(s) 81
BsiHKCI CYCGRG 1 cut(s) 227
BsiSI CCGG 1 cut(s) 168
BslFI GGGAC 1 cut(s) 465
BslI CCNNNNNNNGG 2 cut(s) 97, 490
BsmAI GTCTC 1 cut(s) 575
BsmFI GGGAC 1 cut(s) 465
BsnI GGCC 1 cut(s) 81
BsoBI CYCGRG 1 cut(s) 227
Bsp13I TCCGGA 1 cut(s) 167
Bsp143I GATC 5 cut(s) 268, 326, 399, 575, 652
BspACI CCGC 7 cut(s) 52, 64, 95, 98, 181, 192, 282
BspANI GGCC 1 cut(s) 81
BspCNI CTCAG 4 cut(s) 482, 598, 616, 648
BspEI TCCGGA 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 42, 226
BssMI GATC 5 cut(s) 268, 326, 399, 575, 652
BssT1I CCWWGG 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 2 cut(s) 351, 436
Bst6I CTCTTC 3 cut(s) 125, 573, 660
BstC8I GCNNGC 1 cut(s) 292
BstDEI CTNAG 4 cut(s) 469, 606, 624, 656
BstF5I GGATG 3 cut(s) 445, 655, 714
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 5 cut(s) 271, 329, 402, 578, 655
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 5 cut(s) 268, 326, 399, 575, 652
BstMWI GCNNNNNNNGC 2 cut(s) 38, 695
BstNI CCWGG 1 cut(s) 314
BstSCI CCNGG 1 cut(s) 312
BstSFI CTRYAG 2 cut(s) 70, 330
BstV2I GAAGAC 1 cut(s) 255
BstX2I RGATCY 1 cut(s) 326
BstYI RGATCY 1 cut(s) 326
BsuRI GGCC 1 cut(s) 81
BtrI CACGTC 2 cut(s) 457, 517
BtsCI GGATG 3 cut(s) 445, 655, 714
Cac8I GCNNGC 1 cut(s) 292
CfoI GCGC 1 cut(s) 296
CseI GACGC 1 cut(s) 97
CviAII CATG 1 cut(s) 541
CviJI RGCY 5 cut(s) 41, 81, 689, 734, 755
CviKI_1 RGCY 5 cut(s) 41, 81, 689, 734, 755
DdeI CTNAG 4 cut(s) 469, 606, 624, 656
DpnI GATC 5 cut(s) 270, 328, 401, 577, 654
DpnII GATC 5 cut(s) 268, 326, 399, 575, 652
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 3 cut(s) 125, 573, 660
EarI CTCTTC 3 cut(s) 125, 573, 660
EciI GGCGGA 2 cut(s) 113, 170
Eco130I CCWWGG 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 554
Eco88I CYCGRG 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 312
EcoT14I CCWWGG 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 1 cut(s) 544
FaiI YATR 8 cut(s) 200, 449, 486, 498, 542, 695, 747, 752
FaqI GGGAC 1 cut(s) 465
FatI CATG 1 cut(s) 540
FblI GTMKAC 1 cut(s) 381
Fnu4HI GCNGC 3 cut(s) 53, 96, 192
FokI GGATG 3 cut(s) 452, 662, 721
Fsp4HI GCNGC 3 cut(s) 53, 96, 192
GlaI GCGC 1 cut(s) 295
GluI GCNGC 3 cut(s) 53, 96, 192
HaeIII GGCC 1 cut(s) 81
HapII CCGG 1 cut(s) 168
HgaI GACGC 1 cut(s) 97
HhaI GCGC 1 cut(s) 296
Hin1II CATG 1 cut(s) 544
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HindIII AAGCTT 1 cut(s) 687
HinfI GANTC 9 cut(s) 5, 337, 416, 427, 467, 473, 478, 620, 725
HpaII CCGG 1 cut(s) 168
HphI GGTGA 3 cut(s) 247, 409, 556
Hpy166II GTNNAC 1 cut(s) 382
Hpy188III TCNNGA 6 cut(s) 168, 229, 460, 522, 644, 722
Hpy8I GTNNAC 1 cut(s) 382
Hpy99I CGWCG 8 cut(s) 113, 158, 245, 290, 389, 461, 720, 740
HpyCH4III ACNGT 2 cut(s) 351, 436
HpyCH4IV ACGT 4 cut(s) 24, 456, 516, 562
HpyCH4V TGCA 1 cut(s) 597
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 695
HpyF3I CTNAG 4 cut(s) 469, 606, 624, 656
HpySE526I ACGT 4 cut(s) 24, 456, 516, 562
Hsp92II CATG 1 cut(s) 544
HspAI GCGC 1 cut(s) 294
Kpn2I TCCGGA 1 cut(s) 167
Kzo9I GATC 5 cut(s) 268, 326, 399, 575, 652
LmnI GCTCC 1 cut(s) 170
LpnPI CCDG 6 cut(s) 181, 299, 326, 380, 507, 657
MaeII ACGT 4 cut(s) 24, 456, 516, 562
MaeIII GTNAC 1 cut(s) 403
MalI GATC 5 cut(s) 270, 328, 401, 577, 654
MboI GATC 5 cut(s) 268, 326, 399, 575, 652
MboII GAAGA 9 cut(s) 79, 112, 242, 255, 371, 513, 560, 646, 677
MflI RGATCY 1 cut(s) 326
MluCI AATT 1 cut(s) 75
MlyI GAGTC 3 cut(s) 461, 487, 614
MroI TCCGGA 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 669
MslI CAYNNNNRTG 1 cut(s) 545
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 314
MvaI CCWGG 1 cut(s) 314
MwoI GCNNNNNNNGC 2 cut(s) 38, 695
NdeII GATC 5 cut(s) 268, 326, 399, 575, 652
NlaIII CATG 1 cut(s) 544
PdmI GAANNNNTTC 1 cut(s) 669
PfeI GAWTC 6 cut(s) 5, 337, 416, 427, 473, 725
PkrI GCNGC 3 cut(s) 54, 97, 193
PleI GAGTC 3 cut(s) 461, 486, 614
PpsI GAGTC 3 cut(s) 461, 486, 614
Psp1406I AACGTT 1 cut(s) 562
Psp6I CCWGG 1 cut(s) 312
PspGI CCWGG 1 cut(s) 312
PsuI RGATCY 1 cut(s) 326
RseI CAYNNNNRTG 1 cut(s) 545
SatI GCNGC 3 cut(s) 53, 96, 192
Sau3AI GATC 5 cut(s) 268, 326, 399, 575, 652
SchI GAGTC 3 cut(s) 461, 487, 614
ScrFI CCNGG 1 cut(s) 314
SetI ASST 8 cut(s) 27, 105, 399, 459, 495, 519, 565, 691
SfcI CTRYAG 2 cut(s) 70, 330
SmiMI CAYNNNNRTG 1 cut(s) 545
Sse9I AATT 1 cut(s) 75
SsiI CCGC 7 cut(s) 52, 64, 95, 98, 181, 192, 282
StyD4I CCNGG 1 cut(s) 312
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 2 cut(s) 351, 436
TaiI ACGT 4 cut(s) 27, 459, 519, 565
TaqI TCGA 3 cut(s) 18, 459, 476
TasI AATT 1 cut(s) 75
TauI GCSGC 3 cut(s) 55, 98, 194
TfiI GAWTC 6 cut(s) 5, 337, 416, 427, 473, 725
TspDTI ATGAA 4 cut(s) 112, 372, 572, 682
TspGWI ACGGA 2 cut(s) 80, 383
XmiI GTMKAC 1 cut(s) 381
XmnI GAANNNNTTC 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.