Rmu_sc0001659.1_g000047

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001659.1
OG0009081
Physical Location & Seq
Reverse (-)
194106 .. 194888
783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001659.1_g000047.1.cds

Sequence Viewer

Length: 783 bp
atggactcgtgcagtttcgacaacgtcaaggcggagaaagccagcgccatgcggaggtacaaccgtctccgcaccatcgccaggcttttccgcctcgccgagctctgcgtcgccgccgtcttgctctcctggaccttcactcgcctccccttcgccctccgcatctccctcgactacctccgcctcttctccggcgtcgtttcgagccctctcttcgtcttcctcgtctgcaacgccatcatcgtctctctcttcgccaagtcccgcaacgtcaccgcctccgcctccgcctcgcctcaggatccgaaggacgacgtggagactcaactttacgatgagttcaccaacaactgcggcggaaagatgtcgaactccggtgacgacgtcctctccagtgtgaaagaggaggttgtgtatcaggacaagcagatcatctccgaggtgaacgccgccgatcctaaacctgaaaccgactcgaactccggcgctgggtcagactcggactcggactcggactcgggtttggagtttccgaaagctatccggaggacgaaatcggagaagttcgagagagttgttccggcgaagctaaaaaggtcggacaccgaggattgccggaaaatgctgaggtccggcgaggaaactgtgccggaagatttcgatccagaggacgacttgagcaatgaggagtttcagcgcgccattgaagctttcattgagaagcagaagaggtttcgtcatcaggaagagtctctggccattgtgctacaaaaccagagctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408

Protein Analysis

260

Amino Acids

29.18

Weight (kDa)

4.93

Isoelectric Point (pI)

62.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 387
AccII CGCG 1 cut(s) 699
AccIII TCCGGA 1 cut(s) 543
AclWI GGATC 4 cut(s) 296, 309, 449, 656
AcoI YGGCCR 1 cut(s) 756
AcyI GRCGYC 2 cut(s) 195, 384
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 3 cut(s) 54, 81, 489
AgsI TTSAA 1 cut(s) 707
AjiI CACGTC 1 cut(s) 316
AjnI CCWGG 2 cut(s) 80, 128
AluBI AGCT 5 cut(s) 103, 539, 589, 710, 780
AluI AGCT 5 cut(s) 103, 539, 589, 710, 780
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 4 cut(s) 71, 250, 314, 756
AlwI GGATC 4 cut(s) 296, 309, 449, 656
Ama87I CYCGRG 1 cut(s) 517
Aor13HI TCCGGA 1 cut(s) 543
AoxI GGCC 1 cut(s) 756
AspLEI GCGC 4 cut(s) 47, 488, 699, 701
AspS9I GGNCC 2 cut(s) 132, 630
AsuHPI GGTGA 4 cut(s) 265, 334, 389, 454
AvaI CYCGRG 1 cut(s) 517
AvaII GGWCC 2 cut(s) 132, 630
AxyI CCTNAGG 1 cut(s) 297
BaeI ACNNNNGTAYC 2 cut(s) 49, 82
BalI TGGCCA 1 cut(s) 758
BamHI GGATCC 1 cut(s) 301
BanII GRGCYC 2 cut(s) 105, 209
BauI CACGAG 1 cut(s) 7
BbsI GAAGAC 1 cut(s) 211
Bbv12I GWGCWC 1 cut(s) 105
BbvCI CCTCAGC 1 cut(s) 626
BccI CCATC 2 cut(s) 83, 245
BceAI ACGGC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BciT130I CCWGG 2 cut(s) 82, 130
BcoDI GTCTC 4 cut(s) 71, 250, 314, 756
BfoI RGCGCY 2 cut(s) 48, 489
BisI GCNGC 3 cut(s) 114, 355, 450
BlsI GCNGC 3 cut(s) 115, 356, 451
Bme1390I CCNGG 2 cut(s) 82, 130
Bme18I GGWCC 2 cut(s) 132, 630
BmeT110I CYCGRG 1 cut(s) 517
BmgBI CACGTC 1 cut(s) 316
BmgT120I GGNCC 2 cut(s) 132, 630
BmiI GGNNCC 1 cut(s) 303
BmrFI CCNGG 2 cut(s) 82, 130
BmsI GCATC 1 cut(s) 171
BpiI GAAGAC 1 cut(s) 211
BpmI CTGGAG 1 cut(s) 376
Bpu10I CCTNAGC 1 cut(s) 626
BpuEI CTTGAG 1 cut(s) 697
BsaBI GATNNNNATC 1 cut(s) 660
BsaHI GRCGYC 2 cut(s) 195, 384
BsaJI CCNNGG 2 cut(s) 438, 606
BsaWI WCCGGW 2 cut(s) 374, 543
BsaXI ACNNNNNCTCC 6 cut(s) 374, 398, 404, 428, 464, 494
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 81, 489
Bse1I ACTGG 1 cut(s) 393
Bse21I CCTNAGG 1 cut(s) 297
Bse3DI GCAATG 1 cut(s) 688
Bse8I GATNNNNATC 1 cut(s) 660
BseAI TCCGGA 1 cut(s) 543
BseBI CCWGG 2 cut(s) 82, 130
BseDI CCNNGG 2 cut(s) 438, 606
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 3 cut(s) 54, 81, 489
BseMI GCAATG 1 cut(s) 688
BseMII CTCAG 2 cut(s) 311, 617
BseNI ACTGG 1 cut(s) 393
BsePI GCGCGC 1 cut(s) 697
BseRI GAGGAG 2 cut(s) 419, 701
BseYI CCCAGC 1 cut(s) 488
BsgI GTGCAG 1 cut(s) 31
Bsh1236I CGCG 1 cut(s) 699
BshFI GGCC 1 cut(s) 758
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 517
BsiSI CCGG 8 cut(s) 192, 375, 483, 544, 581, 616, 633, 650
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 3 cut(s) 54, 81, 489
BsmAI GTCTC 4 cut(s) 71, 250, 314, 756
BsmBI CGTCTC 2 cut(s) 71, 250
BsmFI GGGAC 1 cut(s) 247
BsnI GGCC 1 cut(s) 758
BsoBI CYCGRG 1 cut(s) 517
Bsp1286I GDGCHC 2 cut(s) 105, 209
Bsp13I TCCGGA 1 cut(s) 543
Bsp143I GATC 4 cut(s) 301, 429, 454, 661
BspANI GGCC 1 cut(s) 758
BspCNI CTCAG 2 cut(s) 310, 618
BspEI TCCGGA 1 cut(s) 543
BspFNI CGCG 1 cut(s) 699
BspLI GGNNCC 1 cut(s) 303
BspPI GGATC 4 cut(s) 296, 309, 449, 656
BsrDI GCAATG 1 cut(s) 688
BsrI ACTGG 1 cut(s) 393
BssECI CCNNGG 2 cut(s) 438, 606
BssHII GCGCGC 1 cut(s) 697
BssMI GATC 4 cut(s) 301, 429, 454, 661
BssNI GRCGYC 2 cut(s) 195, 384
BssSI CACGAG 1 cut(s) 7
Bst2BI CACGAG 1 cut(s) 7
Bst2UI CCWGG 2 cut(s) 82, 130
Bst4CI ACNGT 2 cut(s) 65, 646
Bst6I CTCTTC 5 cut(s) 191, 218, 257, 722, 741
BstACI GRCGYC 2 cut(s) 195, 384
BstC8I GCNNGC 2 cut(s) 43, 699
BstDEI CTNAG 2 cut(s) 297, 626
BstFNI CGCG 1 cut(s) 699
BstH2I RGCGCY 2 cut(s) 48, 489
BstHHI GCGC 4 cut(s) 47, 488, 699, 701
BstKTI GATC 4 cut(s) 304, 432, 457, 664
BstMAI GTCTC 4 cut(s) 71, 250, 314, 756
BstMBI GATC 4 cut(s) 301, 429, 454, 661
BstMWI GCNNNNNNNGC 2 cut(s) 38, 707
BstNI CCWGG 2 cut(s) 82, 130
BstSCI CCNGG 2 cut(s) 80, 128
BstUI CGCG 1 cut(s) 699
BstV2I GAAGAC 1 cut(s) 211
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
Bsu36I CCTNAGG 1 cut(s) 297
BsuRI GGCC 1 cut(s) 758
BtgZI GCGATG 1 cut(s) 61
BtrI CACGTC 1 cut(s) 316
BtsIMutI CAGTG 1 cut(s) 400
Cac8I GCNNGC 2 cut(s) 43, 699
CfoI GCGC 4 cut(s) 47, 488, 699, 701
Cfr13I GGNCC 2 cut(s) 132, 630
CseI GACGC 2 cut(s) 97, 184
Csp6I GTAC 1 cut(s) 58
CviAII CATG 1 cut(s) 49
CviJI RGCY 9 cut(s) 41, 85, 103, 207, 539, 589, 710, 758, 780
CviKI_1 RGCY 9 cut(s) 41, 85, 103, 207, 539, 589, 710, 758, 780
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 2 cut(s) 297, 626
DpnI GATC 4 cut(s) 303, 431, 456, 663
DpnII GATC 4 cut(s) 301, 429, 454, 661
EaeI YGGCCR 1 cut(s) 756
Eam1104I CTCTTC 5 cut(s) 191, 218, 257, 722, 741
EarI CTCTTC 5 cut(s) 191, 218, 257, 722, 741
EciI GGCGGA 6 cut(s) 47, 80, 170, 271, 277, 372
Ecl136II GAGCTC 1 cut(s) 103
Eco24I GRGCYC 2 cut(s) 105, 209
Eco47I GGWCC 2 cut(s) 132, 630
Eco53kI GAGCTC 1 cut(s) 103
Eco81I CCTNAGG 1 cut(s) 297
Eco88I CYCGRG 1 cut(s) 517
EcoICRI GAGCTC 1 cut(s) 103
EcoRII CCWGG 2 cut(s) 80, 128
EcoT38I GRGCYC 2 cut(s) 105, 209
Esp3I CGTCTC 2 cut(s) 71, 250
FaeI CATG 1 cut(s) 52
FaiI YATR 1 cut(s) 50
FaqI GGGAC 1 cut(s) 247
FatI CATG 1 cut(s) 48
FauI CCCGC 1 cut(s) 272
Fnu4HI GCNGC 3 cut(s) 114, 355, 450
FriOI GRGCYC 2 cut(s) 105, 209
Fsp4HI GCNGC 3 cut(s) 114, 355, 450
GlaI GCGC 4 cut(s) 46, 487, 698, 700
GluI GCNGC 3 cut(s) 114, 355, 450
GsaI CCCAGC 1 cut(s) 492
GsuI CTGGAG 1 cut(s) 376
HaeII RGCGCY 2 cut(s) 48, 489
HaeIII GGCC 1 cut(s) 758
HapII CCGG 8 cut(s) 192, 375, 483, 544, 581, 616, 633, 650
HgaI GACGC 2 cut(s) 97, 184
HhaI GCGC 4 cut(s) 47, 488, 699, 701
Hin1I GRCGYC 2 cut(s) 195, 384
Hin1II CATG 1 cut(s) 52
Hin6I GCGC 4 cut(s) 45, 486, 697, 699
HinP1I GCGC 4 cut(s) 45, 486, 697, 699
HindIII AAGCTT 1 cut(s) 708
HinfI GANTC 8 cut(s) 5, 322, 473, 497, 503, 509, 515, 749
HpaII CCGG 8 cut(s) 192, 375, 483, 544, 581, 616, 633, 650
HphI GGTGA 4 cut(s) 265, 334, 389, 454
Hpy166II GTNNAC 2 cut(s) 342, 445
Hpy188I TCNGA 9 cut(s) 306, 439, 496, 502, 508, 514, 534, 559, 601
Hpy188III TCNNGA 6 cut(s) 299, 419, 544, 568, 665, 743
Hpy8I GTNNAC 2 cut(s) 342, 445
Hpy99I CGWCG 4 cut(s) 113, 200, 317, 386
HpyAV CCTTC 3 cut(s) 145, 160, 301
HpyCH4III ACNGT 2 cut(s) 65, 646
HpyCH4IV ACGT 4 cut(s) 24, 270, 315, 384
HpyCH4V TGCA 2 cut(s) 12, 231
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 707
HpyF3I CTNAG 2 cut(s) 297, 626
HpySE526I ACGT 4 cut(s) 24, 270, 315, 384
Hsp92I GRCGYC 2 cut(s) 195, 384
Hsp92II CATG 1 cut(s) 52
HspAI GCGC 4 cut(s) 45, 486, 697, 699
Kpn2I TCCGGA 1 cut(s) 543
Kzo9I GATC 4 cut(s) 301, 429, 454, 661
LweI GCATC 1 cut(s) 171
MaeII ACGT 4 cut(s) 24, 270, 315, 384
MaeIII GTNAC 2 cut(s) 271, 377
MalI GATC 4 cut(s) 303, 431, 456, 663
MboI GATC 4 cut(s) 301, 429, 454, 661
MboII GAAGA 7 cut(s) 178, 205, 211, 244, 665, 739, 758
MflI RGATCY 1 cut(s) 301
MhlI GDGCHC 2 cut(s) 105, 209
MlsI TGGCCA 1 cut(s) 758
MluNI TGGCCA 1 cut(s) 758
MlyI GAGTC 7 cut(s) 316, 467, 491, 497, 503, 509, 758
MmeI TCCRAC 1 cut(s) 579
Mox20I TGGCCA 1 cut(s) 758
MroI TCCGGA 1 cut(s) 543
MscI TGGCCA 1 cut(s) 758
Msp20I TGGCCA 1 cut(s) 758
MspI CCGG 8 cut(s) 192, 375, 483, 544, 581, 616, 633, 650
MspR9I CCNGG 2 cut(s) 82, 130
MvaI CCWGG 2 cut(s) 82, 130
MvnI CGCG 1 cut(s) 699
MwoI GCNNNNNNNGC 2 cut(s) 38, 707
NdeII GATC 4 cut(s) 301, 429, 454, 661
NlaIII CATG 1 cut(s) 52
NlaIV GGNNCC 1 cut(s) 303
NmeAIII GCCGAG 1 cut(s) 124
NmuCI GTSAC 2 cut(s) 271, 377
PauI GCGCGC 1 cut(s) 697
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 240
PflFI GACNNNGTC 2 cut(s) 23, 383
PfoI TCCNGGA 1 cut(s) 128
PkrI GCNGC 3 cut(s) 115, 356, 451
PleI GAGTC 7 cut(s) 316, 467, 491, 497, 503, 509, 757
PpsI GAGTC 7 cut(s) 316, 467, 491, 497, 503, 509, 757
Psp124BI GAGCTC 1 cut(s) 105
Psp6I CCWGG 2 cut(s) 80, 128
PspFI CCCAGC 1 cut(s) 488
PspGI CCWGG 2 cut(s) 80, 128
PspN4I GGNNCC 1 cut(s) 303
PspPI GGNCC 2 cut(s) 132, 630
PsuI RGATCY 1 cut(s) 301
PsyI GACNNNGTC 2 cut(s) 23, 383
PteI GCGCGC 1 cut(s) 697
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
SacI GAGCTC 1 cut(s) 105
SatI GCNGC 3 cut(s) 114, 355, 450
Sau3AI GATC 4 cut(s) 301, 429, 454, 661
Sau96I GGNCC 2 cut(s) 132, 630
SchI GAGTC 7 cut(s) 316, 467, 491, 497, 503, 509, 758
ScrFI CCNGG 2 cut(s) 82, 130
SduI GDGCHC 2 cut(s) 105, 209
SfaNI GCATC 1 cut(s) 171
SinI GGWCC 2 cut(s) 132, 630
SmlI CTYRAG 1 cut(s) 676
SmoI CTYRAG 1 cut(s) 676
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 2 cut(s) 80, 128
TaaI ACNGT 2 cut(s) 65, 646
TaiI ACGT 4 cut(s) 27, 273, 318, 387
TaqI TCGA 7 cut(s) 18, 171, 203, 368, 476, 567, 660
TauI GCSGC 3 cut(s) 116, 357, 452
TscAI CASTG 1 cut(s) 400
TseFI GTSAC 2 cut(s) 271, 377
Tsp45I GTSAC 2 cut(s) 271, 377
TspDTI ATGAA 1 cut(s) 703
TspRI CASTG 1 cut(s) 400
Tth111I GACNNNGTC 2 cut(s) 23, 383
VpaK11BI GGWCC 2 cut(s) 132, 630
ZraI GACGTC 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.