Rmu_sc0008053.1_g000019

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008053.1
OG0009081
Physical Location & Seq
Reverse (-)
70930 .. 71322
393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008053.1_g000019.1.cds

Sequence Viewer

Length: 393 bp
atggactcgtgcagtttcgacaacgtcaaggcggagaaagctagcgccatgcggaggtacaaccgtctccgcaccatcgccaggcttttttgcctcaccgagctctgcgtcgccgccgtcttgctctcctggaccttcactcgcctccccttcgccctcctcatctccctcgactacctccacctcttctccggtgtcgtttcgagccctctctttgtcttcctcgtctgcaacgccatcatcgtctctctcttcgccaagtcccccaacgtcaccgcctccgcctcgcctcgggacccgaaggacgacgtggagactcagctttacgatgagttcacaacaactgcggtggaaagatgtcgaacttctgtaacgacgtcctctccactgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408

Protein Analysis

130

Amino Acids

14.47

Weight (kDa)

7.61

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 380
AciI CCGC 7 cut(s) 32, 52, 70, 114, 276, 282, 347
AcyI GRCGYC 1 cut(s) 377
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 3 cut(s) 54, 81, 291
AjiI CACGTC 1 cut(s) 310
AjnI CCWGG 2 cut(s) 80, 128
AluBI AGCT 3 cut(s) 41, 103, 322
AluI AGCT 3 cut(s) 41, 103, 322
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 3 cut(s) 71, 250, 308
Ama87I CYCGRG 1 cut(s) 291
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 2 cut(s) 132, 295
AsuHPI GGTGA 2 cut(s) 88, 265
AsuNHI GCTAGC 1 cut(s) 41
AvaI CYCGRG 1 cut(s) 291
AvaII GGWCC 2 cut(s) 132, 295
BaeI ACNNNNGTAYC 2 cut(s) 49, 82
BanII GRGCYC 2 cut(s) 105, 209
BauI CACGAG 1 cut(s) 7
BbsI GAAGAC 1 cut(s) 211
Bbv12I GWGCWC 1 cut(s) 105
BccI CCATC 2 cut(s) 83, 245
BceAI ACGGC 1 cut(s) 101
BciT130I CCWGG 2 cut(s) 82, 130
BcoDI GTCTC 3 cut(s) 71, 250, 308
BfaI CTAG 1 cut(s) 42
BfoI RGCGCY 1 cut(s) 48
BisI GCNGC 1 cut(s) 114
BlsI GCNGC 1 cut(s) 115
Bme1390I CCNGG 2 cut(s) 82, 130
Bme18I GGWCC 2 cut(s) 132, 295
BmeT110I CYCGRG 1 cut(s) 291
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 2 cut(s) 132, 295
BmiI GGNNCC 2 cut(s) 296, 297
BmrFI CCNGG 2 cut(s) 82, 130
BmtI GCTAGC 1 cut(s) 45
BpiI GAAGAC 1 cut(s) 211
BsaHI GRCGYC 1 cut(s) 377
BsaJI CCNNGG 1 cut(s) 290
BsaWI WCCGGW 1 cut(s) 191
BsaXI ACNNNNNCTCC 3 cut(s) 173, 203, 367
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 81, 291
BseBI CCWGG 2 cut(s) 82, 130
BseDI CCNNGG 1 cut(s) 290
BseLI CCNNNNNNNGG 3 cut(s) 54, 81, 291
BseMII CTCAG 1 cut(s) 332
BseRI GAGGAG 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 291
BsiSI CCGG 1 cut(s) 192
BslFI GGGAC 2 cut(s) 247, 308
BslI CCNNNNNNNGG 3 cut(s) 54, 81, 291
BsmAI GTCTC 3 cut(s) 71, 250, 308
BsmBI CGTCTC 2 cut(s) 71, 250
BsmFI GGGAC 2 cut(s) 247, 308
BsoBI CYCGRG 1 cut(s) 291
Bsp1286I GDGCHC 2 cut(s) 105, 209
BspACI CCGC 7 cut(s) 32, 52, 70, 114, 276, 282, 347
BspCNI CTCAG 1 cut(s) 331
BspLI GGNNCC 2 cut(s) 296, 297
BspOI GCTAGC 1 cut(s) 45
BssECI CCNNGG 1 cut(s) 290
BssNI GRCGYC 1 cut(s) 377
BssSI CACGAG 1 cut(s) 7
Bst2BI CACGAG 1 cut(s) 7
Bst2UI CCWGG 2 cut(s) 82, 130
Bst4CI ACNGT 2 cut(s) 65, 390
Bst6I CTCTTC 2 cut(s) 191, 257
BstACI GRCGYC 1 cut(s) 377
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 1 cut(s) 318
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 1 cut(s) 47
BstMAI GTCTC 3 cut(s) 71, 250, 308
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstNI CCWGG 2 cut(s) 82, 130
BstSCI CCNGG 2 cut(s) 80, 128
BstV2I GAAGAC 1 cut(s) 211
BtgZI GCGATG 1 cut(s) 61
BtrI CACGTC 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 386
Cac8I GCNNGC 1 cut(s) 43
CfoI GCGC 1 cut(s) 47
Cfr13I GGNCC 2 cut(s) 132, 295
CseI GACGC 1 cut(s) 97
Csp6I GTAC 1 cut(s) 58
CspCI CAANNNNNGTGG 2 cut(s) 330, 365
CviAII CATG 1 cut(s) 49
CviJI RGCY 5 cut(s) 41, 85, 103, 207, 322
CviKI_1 RGCY 5 cut(s) 41, 85, 103, 207, 322
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 1 cut(s) 318
Eam1104I CTCTTC 2 cut(s) 191, 257
EarI CTCTTC 2 cut(s) 191, 257
EciI GGCGGA 2 cut(s) 47, 271
Ecl136II GAGCTC 1 cut(s) 103
Eco24I GRGCYC 2 cut(s) 105, 209
Eco47I GGWCC 2 cut(s) 132, 295
Eco53kI GAGCTC 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 291
EcoICRI GAGCTC 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 295
EcoRII CCWGG 2 cut(s) 80, 128
EcoT38I GRGCYC 2 cut(s) 105, 209
Esp3I CGTCTC 2 cut(s) 71, 250
FaeI CATG 1 cut(s) 52
FaiI YATR 1 cut(s) 50
FaqI GGGAC 2 cut(s) 247, 308
FatI CATG 1 cut(s) 48
Fnu4HI GCNGC 1 cut(s) 114
FriOI GRGCYC 2 cut(s) 105, 209
Fsp4HI GCNGC 1 cut(s) 114
FspBI CTAG 1 cut(s) 42
GlaI GCGC 1 cut(s) 46
GluI GCNGC 1 cut(s) 114
HaeII RGCGCY 1 cut(s) 48
HapII CCGG 1 cut(s) 192
HgaI GACGC 1 cut(s) 97
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 377
Hin1II CATG 1 cut(s) 52
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HinfI GANTC 2 cut(s) 5, 316
HpaII CCGG 1 cut(s) 192
HphI GGTGA 2 cut(s) 88, 265
Hpy166II GTNNAC 1 cut(s) 336
Hpy188III TCNNGA 1 cut(s) 293
Hpy8I GTNNAC 1 cut(s) 336
Hpy99I CGWCG 3 cut(s) 113, 311, 379
HpyAV CCTTC 3 cut(s) 145, 160, 295
HpyCH4III ACNGT 2 cut(s) 65, 390
HpyCH4IV ACGT 4 cut(s) 24, 270, 309, 377
HpyCH4V TGCA 2 cut(s) 12, 231
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 4 cut(s) 24, 270, 309, 377
Hsp92I GRCGYC 1 cut(s) 377
Hsp92II CATG 1 cut(s) 52
HspAI GCGC 1 cut(s) 45
KflI GGGWCCC 1 cut(s) 295
LpnPI CCDG 5 cut(s) 67, 94, 115, 142, 205
MaeI CTAG 1 cut(s) 42
MaeII ACGT 4 cut(s) 24, 270, 309, 377
MaeIII GTNAC 2 cut(s) 271, 370
MboII GAAGA 3 cut(s) 178, 211, 244
MhlI GDGCHC 2 cut(s) 105, 209
MlyI GAGTC 1 cut(s) 310
MspI CCGG 1 cut(s) 192
MspR9I CCNGG 2 cut(s) 82, 130
MvaI CCWGG 2 cut(s) 82, 130
MwoI GCNNNNNNNGC 1 cut(s) 38
NheI GCTAGC 1 cut(s) 41
NlaIII CATG 1 cut(s) 52
NlaIV GGNNCC 2 cut(s) 296, 297
NmuCI GTSAC 1 cut(s) 271
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PflFI GACNNNGTC 1 cut(s) 23
PfoI TCCNGGA 1 cut(s) 128
PkrI GCNGC 1 cut(s) 115
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
PpuMI RGGWCCY 1 cut(s) 295
Psp124BI GAGCTC 1 cut(s) 105
Psp5II RGGWCCY 1 cut(s) 295
Psp6I CCWGG 2 cut(s) 80, 128
PspGI CCWGG 2 cut(s) 80, 128
PspN4I GGNNCC 2 cut(s) 296, 297
PspPI GGNCC 2 cut(s) 132, 295
PspPPI RGGWCCY 1 cut(s) 295
PsyI GACNNNGTC 1 cut(s) 23
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
SacI GAGCTC 1 cut(s) 105
SatI GCNGC 1 cut(s) 114
Sau96I GGNCC 2 cut(s) 132, 295
SchI GAGTC 1 cut(s) 310
ScrFI CCNGG 2 cut(s) 82, 130
SduI GDGCHC 2 cut(s) 105, 209
SinI GGWCC 2 cut(s) 132, 295
SsiI CCGC 7 cut(s) 32, 52, 70, 114, 276, 282, 347
SspMI CTAG 1 cut(s) 42
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 2 cut(s) 80, 128
TaaI ACNGT 2 cut(s) 65, 390
TaiI ACGT 4 cut(s) 27, 273, 312, 380
TaqI TCGA 4 cut(s) 18, 171, 203, 361
TauI GCSGC 1 cut(s) 116
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 271
Tsp45I GTSAC 1 cut(s) 271
TspRI CASTG 1 cut(s) 393
Tth111I GACNNNGTC 1 cut(s) 23
VpaK11BI GGWCC 2 cut(s) 132, 295
XspI CTAG 1 cut(s) 42
ZraI GACGTC 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.