Rorug02G0093600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009081
Physical Location & Seq
Reverse (-)
7675618 .. 7676773
1156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0093600.1

Sequence Viewer

Length: 447 bp
ATGGCTATCAGCGAGTCTCATCCCCAAGCCTCCACCAAACCAAACCATTCTCCTCCAATGGAAGTCCACTGCGTCAAATGCGACTCCTGCGGCTTCACAGAGGATTGCACCCCCGCGTACATAGCACGCGTCCGTGAGCGCTACCAGGGACGGTGGATATGTGGGCTCTGCGTGGAGGCCGTGAAAGACGAGGCGCTCCGATCAGATAGGCTCATCTCCACCGAGGAGGCCCTGAACCGCCACATAAGCTTCTGCAAGAATTTCAGATCGTCTAGTAAGGAAAAAACAGAGCACCCTATTCTGGCCATGGGCCGGGTTTTCCGCCGGAGCTTGGATACTCCGAGGGCGCTCCGGTCCAATTCTAGCTCCTCTCTCGGTGGCGTTGAGAGGGTTGGTGGGCCGGCGCTGGTTAGGTCCGGCAGTTGCTTCCCTGCTCTCAATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408

Protein Analysis

148

Amino Acids

16.33

Weight (kDa)

8.88

Isoelectric Point (pI)

72.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 23 - 113 1.3e-36 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 116, 129
AciI CCGC 4 cut(s) 90, 114, 238, 322
AcoI YGGCCR 1 cut(s) 303
AcsI RAATTY 1 cut(s) 259
AfaI GTAC 1 cut(s) 119
AfeI AGCGCT 1 cut(s) 140
AfiI CCNNNNNNNGG 3 cut(s) 301, 312, 331
AflIII ACRYGT 1 cut(s) 127
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 4 cut(s) 249, 330, 366, 444
AluI AGCT 4 cut(s) 249, 330, 366, 444
Alw21I GWGCWC 1 cut(s) 294
Alw26I GTCTC 1 cut(s) 21
Aor51HI AGCGCT 1 cut(s) 140
AoxI GGCC 5 cut(s) 177, 228, 303, 310, 398
ApoI RAATTY 1 cut(s) 259
AspLEI GCGC 4 cut(s) 141, 196, 349, 406
AspS9I GGNCC 5 cut(s) 229, 310, 354, 398, 414
AsuC2I CCSGG 1 cut(s) 314
AvaII GGWCC 2 cut(s) 354, 414
BalI TGGCCA 1 cut(s) 305
BanII GRGCYC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 294
BceAI ACGGC 1 cut(s) 164
BciT130I CCWGG 1 cut(s) 146
BciVI GTATCC 1 cut(s) 328
BcnI CCSGG 1 cut(s) 314
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 2 cut(s) 273, 363
BfoI RGCGCY 4 cut(s) 142, 197, 350, 407
BfuI GTATCC 1 cut(s) 328
BisI GCNGC 1 cut(s) 91
BlsI GCNGC 1 cut(s) 92
Bme1390I CCNGG 2 cut(s) 146, 314
Bme18I GGWCC 2 cut(s) 354, 414
BmgT120I GGNCC 5 cut(s) 229, 310, 354, 398, 414
BmrFI CCNGG 2 cut(s) 146, 314
BpuMI CCSGG 1 cut(s) 314
BsaJI CCNNGG 4 cut(s) 145, 222, 306, 341
BsaWI WCCGGW 1 cut(s) 351
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 312, 331
Bse118I RCCGGY 1 cut(s) 400
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 4 cut(s) 145, 222, 306, 341
BseGI GGATG 1 cut(s) 19
BseLI CCNNNNNNNGG 3 cut(s) 301, 312, 331
BseRI GAGGAG 3 cut(s) 42, 239, 358
Bsh1236I CGCG 2 cut(s) 116, 129
BshFI GGCC 5 cut(s) 179, 230, 305, 312, 400
BsiHKAI GWGCWC 1 cut(s) 294
BsiSI CCGG 5 cut(s) 313, 325, 352, 401, 417
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 3 cut(s) 301, 312, 331
BsmAI GTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 162
BsnI GGCC 5 cut(s) 179, 230, 305, 312, 400
Bsp1286I GDGCHC 2 cut(s) 168, 294
Bsp143I GATC 2 cut(s) 200, 266
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 4 cut(s) 90, 114, 238, 322
BspANI GGCC 5 cut(s) 179, 230, 305, 312, 400
BspFNI CGCG 2 cut(s) 116, 129
BsrFI RCCGGY 1 cut(s) 400
BssAI RCCGGY 1 cut(s) 400
BssECI CCNNGG 4 cut(s) 145, 222, 306, 341
BssMI GATC 2 cut(s) 200, 266
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 153
BstC8I GCNNGC 2 cut(s) 127, 402
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 1 cut(s) 19
BstFNI CGCG 2 cut(s) 116, 129
BstH2I RGCGCY 4 cut(s) 142, 197, 350, 407
BstHHI GCGC 4 cut(s) 141, 196, 349, 406
BstKTI GATC 2 cut(s) 203, 269
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 2 cut(s) 200, 266
BstMWI GCNNNNNNNGC 4 cut(s) 78, 87, 122, 246
BstNI CCWGG 1 cut(s) 146
BstSCI CCNGG 2 cut(s) 144, 312
BstUI CGCG 2 cut(s) 116, 129
BsuI GTATCC 1 cut(s) 328
BsuRI GGCC 5 cut(s) 179, 230, 305, 312, 400
BtgI CCRYGG 1 cut(s) 306
BtsCI GGATG 1 cut(s) 19
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 1 cut(s) 67
Cac8I GCNNGC 2 cut(s) 127, 402
CfoI GCGC 4 cut(s) 141, 196, 349, 406
Cfr10I RCCGGY 1 cut(s) 400
Cfr13I GGNCC 5 cut(s) 229, 310, 354, 398, 414
CseI GACGC 2 cut(s) 61, 118
Csp6I GTAC 1 cut(s) 118
CviAII CATG 1 cut(s) 307
CviQI GTAC 1 cut(s) 118
DpnI GATC 2 cut(s) 202, 268
DpnII GATC 2 cut(s) 200, 266
EaeI YGGCCR 1 cut(s) 303
EciI GGCGGA 1 cut(s) 311
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 168
Eco47I GGWCC 2 cut(s) 354, 414
Eco47III AGCGCT 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 229
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 1 cut(s) 306
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 1 cut(s) 310
FaiI YATR 4 cut(s) 122, 160, 245, 308
FaqI GGGAC 1 cut(s) 162
FatI CATG 1 cut(s) 306
FauI CCCGC 1 cut(s) 121
Fnu4HI GCNGC 1 cut(s) 91
FokI GGATG 1 cut(s) 6
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 1 cut(s) 91
FspBI CTAG 2 cut(s) 273, 363
GlaI GCGC 4 cut(s) 140, 195, 348, 405
GluI GCNGC 1 cut(s) 91
HaeII RGCGCY 4 cut(s) 142, 197, 350, 407
HaeIII GGCC 5 cut(s) 179, 230, 305, 312, 400
HapII CCGG 5 cut(s) 313, 325, 352, 401, 417
HgaI GACGC 2 cut(s) 61, 118
HhaI GCGC 4 cut(s) 141, 196, 349, 406
Hin1II CATG 1 cut(s) 310
Hin6I GCGC 4 cut(s) 139, 194, 347, 404
HinP1I GCGC 4 cut(s) 139, 194, 347, 404
HindIII AAGCTT 1 cut(s) 247
HinfI GANTC 2 cut(s) 14, 83
HpaII CCGG 5 cut(s) 313, 325, 352, 401, 417
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 4 cut(s) 200, 205, 266, 342
Hpy8I GTNNAC 1 cut(s) 67
HpyCH4III ACNGT 1 cut(s) 153
HpyCH4V TGCA 2 cut(s) 108, 255
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 87, 122, 246
Hsp92II CATG 1 cut(s) 310
HspAI GCGC 4 cut(s) 139, 194, 347, 404
KroI GCCGGC 1 cut(s) 400
KroNI GCCGGC 1 cut(s) 402
Kzo9I GATC 2 cut(s) 200, 266
LmnI GCTCC 4 cut(s) 201, 327, 354, 371
MaeI CTAG 2 cut(s) 273, 363
MalI GATC 2 cut(s) 202, 268
MboI GATC 2 cut(s) 200, 266
MhlI GDGCHC 2 cut(s) 168, 294
MlsI TGGCCA 1 cut(s) 305
MluCI AATT 2 cut(s) 259, 358
MluI ACGCGT 1 cut(s) 127
MluNI TGGCCA 1 cut(s) 305
MlyI GAGTC 2 cut(s) 23, 77
Mox20I TGGCCA 1 cut(s) 305
MroNI GCCGGC 1 cut(s) 400
MscI TGGCCA 1 cut(s) 305
Msp20I TGGCCA 1 cut(s) 305
MspI CCGG 5 cut(s) 313, 325, 352, 401, 417
MspR9I CCNGG 2 cut(s) 146, 314
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 2 cut(s) 116, 129
MwoI GCNNNNNNNGC 4 cut(s) 78, 87, 122, 246
NaeI GCCGGC 1 cut(s) 402
NciI CCSGG 1 cut(s) 314
NcoI CCATGG 1 cut(s) 306
NdeII GATC 2 cut(s) 200, 266
NgoMIV GCCGGC 1 cut(s) 400
NlaIII CATG 1 cut(s) 310
PdiI GCCGGC 1 cut(s) 402
PkrI GCNGC 1 cut(s) 92
PleI GAGTC 2 cut(s) 22, 77
PpsI GAGTC 2 cut(s) 22, 77
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspPI GGNCC 5 cut(s) 229, 310, 354, 398, 414
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
SatI GCNGC 1 cut(s) 91
Sau3AI GATC 2 cut(s) 200, 266
Sau96I GGNCC 5 cut(s) 229, 310, 354, 398, 414
SchI GAGTC 2 cut(s) 23, 77
ScrFI CCNGG 2 cut(s) 146, 314
SduI GDGCHC 2 cut(s) 168, 294
SetI ASST 5 cut(s) 251, 332, 368, 416, 446
SinI GGWCC 2 cut(s) 354, 414
Sse9I AATT 2 cut(s) 259, 358
SsiI CCGC 4 cut(s) 90, 114, 238, 322
SspMI CTAG 2 cut(s) 273, 363
StyD4I CCNGG 2 cut(s) 144, 312
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 1 cut(s) 153
TasI AATT 2 cut(s) 259, 358
TauI GCSGC 1 cut(s) 93
TscAI CASTG 1 cut(s) 74
TspGWI ACGGA 1 cut(s) 122
TspRI CASTG 1 cut(s) 74
VpaK11BI GGWCC 2 cut(s) 354, 414
XapI RAATTY 1 cut(s) 259
XspI CTAG 2 cut(s) 273, 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.