Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0003680

Physical Location & Seq

Genomic Coordinates Forward (+)

4374944 .. 4379074

Gene Structure 4131 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g08260.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2289 bp

ATGGAAGAGAAGAAACCACCACCAAACGACGTCGTCGTGCAAATTTCCGGCGCCGATGACTCCTTCATGTTCTCGCCGCAGCCTTCCCGAACTGGGTCTCCTCAAAAAGACTCCAACTTTGAGCTCACCGAGCTCAGATTCCCGGCCGGCGCTACTTCGCCGGAGATCACAGCCACCACCGCCGCCAAGCCGCCGCGAATTCCCGGCGAGCTTCGCCGCGTCTCGCTTGCCAGGTCAGCATTCTCCAAACCCAAGTCGAGGTTTCAGGAGCCGGTTTATACTAATGAAGTCAAGGTGACTGAAGAAACTCCTCAGAGAAAGTCAAGTGCAAAGTCACCCAATGTGGGTTCACCAATCACTAGAACCACGGCTACTACTCCTAGAGAAGGTGTGAGATCGACCCCTATAACGCCGAGGACGCCGCTGATTGGTACACCGGCGCGTGGAGAAGAGGAGGAGGAGGATGATGAGGAGGTGTACAAGACTGCCAATCTTAAGGTGCACCAGAAGAGTGGTAAGAAGAAGGTGAAGAAGTTGGTGTTACTTGAGTGGGTTACATTTATGTCCATTGTTGGGTTTTTGATTGCTTGCTTAACTGTTGATAAGTTAGAGAGTAAGAGTATTTGGTCATTGAGATTGTGGAGATGGTGTGTGCTTGTGTTGGTTGTGTTGTGTGGTAGGTTGGTGACAGAATGGCTGATTAATATCTTGGTTTTCGTGATCGAAACAAATTACTTGCTTAAGAAGAAGGTTTTGTATTTTGTATATGGGTTGAAGAGGAGTGTGCAGGTGTTTATTTGGTTGGGTTTGATTCTACTAGCATGGGGTTTGTTGTTTGATCATGGAGTGAAGAGGTCGAAGAATACGTCTAAGATTCTAGGTTATGTGACTAGGGGTTTGGCTTCTTGTGAGATTGGAGCAGCCATATGGTTGTTGAAGAATTTGTTAGTGAAGCTGTTAGCCACCTCTTTTCAGTCCACCAGGTTCTTTGATCGGATTAAAGAATCGATATTTCATCAGTATGTTCTTCGAAGCCTTTCGGGGCCTCCATTGGTGGAGATGGGCCAGATGGCTGGAAGAAAATTTGGGAAGGATAGTGGCCGGTTGAGTTTCAAAAATCTGCCAAAAACAAAGAATGATGGGAAGGAAGGGCCGAAAGAAGAAGTTATTGACGTAGAAAAGCTTTCGAAAATGAAGCAGGATAAAATTTCTGCTTGGACGATGAAAGGATTGATTAATGTGATAAGTGGTTCTGGATTGTCTACCATCTCCCACACACTAGACGACATCAACGATGAGGAGAGTGAGCAGAAGGATATAGAGATTACTAGTGAGTTTGAAGCACAAGCTGCAGCTTATGACATTTTCCTGAATGTAGCCAAGCGTGGTAGCAAGTACATTGAGGAAGATGACCTCTGTCGTTTCTTGAAAAAAGAAGAAGTCGAGCTCGTATTTCCTCTGTTTGAAGGTGGAGCAGAAACTCGAAAGATAAGAAAAAAGGCTTTAAAGAATTGGCTGGTGAATGTTTACAACGAACGCAAATCATTGGCACATTCCTTAACTGATACTAAAACAGCCATAGAGGAGTTGAATAGGCTTGGCTCAGCTCTTGTACTTGTTGTGATCCTTATCGTATGGTTACTTCTGATGGGATTTTTGACAACCAATATACTGGTCTTTATATCATCACAGCTTTTACTGGTGGTGTTTGTTTTTGGTAACACTGCTAAAACTGTGTTTGAAGCCATCATATTTGTCTTTGTGATGCACCCATTTGATGTAGGGGACCGTTGTGTTGTTGATGGAGTACAGTTGATCGTTGAAGAGATGAACATATTGACAACAATCTTCTTGAAACCTGACAATGAGAAGGTATACTATCCAAATTCAGTACTTGCTACTAAAGCCATCAGCAACTTCTACAGGAGCCCGGAGATGGGTGATGCCGTTGAGTTTGCTGTTGATGTTTCTACAACAATTGACACTATTGCGAACATGAAAACCAGAATAAAAACGTACTTGGAGAGCAAGCCTCAGCACTGGCGTCCTGGGCATAGTGTGGCGGTGAAGGAAATCGAAGATGTTAACAAGATGACAATGGCTCTTTATATTTCTCATACGATAAATTTCCAGAACTATGGAGACAAGACCAGCCGACGAACAGAACTAATCTTAGAGCTGAAGAAAATATTTGAAGACCTTAATATAAAATATCACCTCCTGCCTCAAGAGATTATTCTCACCAAAGTTGGGTCAGCAACTACAGAGCTTCCACCCTCATGGCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel PsbX

Protein Analysis

763

Amino Acids

86.06

Weight (kDa)

9.07

Isoelectric Point (pI)

44.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Msy1	PF25886	183 - 324	7.6e-06	Mechanosensitive ion channel protein Msy1-like, transmembrane domain
MS_channel_2nd	PF00924	584 - 640	2.4e-10	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003680)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g08260 FvH4_1g08260 FvH4_1g08260 FvH4_1g08260
malus_domestica	MD02G1086900.v1.1 MD02G1087000.v1.1 MD15G1214500.v1.1 MD15G1214600.v1.1
prunus_persica	Prupe.7G202700_v2.0.a1
pyrus_communis	pycom02g06990 pycom02g07000 pycom15g19040
rosa_chinensis	RchiOBHm_Chr2g0094331
rosa_laevigata	RLG00000016480
rosa_multiflora	Rmu_sc0003819.1_g000016 Rmu_sc0025410.1_g000001 Rmu_sc0025411.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147230 Rroxscaffold_7G00185070
rosa_rugosa	Rorug02G0043300 Rorug02G0043300
rosa_samantha	Rh2AG091000 Rh2AG091100 Rh2BG090900 Rh2CG093400 Rh2CG093500 Rh2DG089900 Rh6AG505100 Rh6BG272500 Rh6CG273600 Rh6CG521500 Rh6CG521800 Rh6DG266600 Rh6DG507600
rosa_wichuraiana	Rw6G043950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	778
AatII	GACGTC	1 cut(s)	33
Acc36I	ACCTGC	1 cut(s)	778
AccB1I	GGYRCC	1 cut(s)	50
AccI	GTMKAC	2 cut(s)	1262, 1875
AccII	CGCG	3 cut(s)	196, 219, 442
AciI	CCGC	8 cut(s)	77, 180, 183, 191, 194, 217, 422, 2063
AclWI	GGATC	1 cut(s)	1618
AcoI	YGGCCR	2 cut(s)	144, 1099
AcsI	RAATTY	7 cut(s)	42, 198, 940, 1082, 1206, 1885, 2125
AcuI	CTGAAG	2 cut(s)	321, 2201
AcyI	GRCGYC	4 cut(s)	30, 51, 419, 2044
AfaI	GTAC	7 cut(s)	433, 479, 1397, 1614, 1809, 1893, 2018
AflII	CTTAAG	2 cut(s)	494, 740
AhlI	ACTAGT	1 cut(s)	1328
AjnI	CCWGG	3 cut(s)	230, 980, 2047
AloI	GAACNNNNNNTCC	2 cut(s)	82, 114
Alw21I	GWGCWC	4 cut(s)	126, 135, 504, 1449
Alw26I	GTCTC	3 cut(s)	102, 226, 2136
Alw44I	GTGCAC	1 cut(s)	500
AlwI	GGATC	1 cut(s)	1618
AoxI	GGCC	5 cut(s)	144, 1043, 1063, 1099, 1151
ApaLI	GTGCAC	1 cut(s)	500
ApeKI	GCWGC	4 cut(s)	79, 920, 1349, 1352
ApoI	RAATTY	7 cut(s)	42, 198, 940, 1082, 1206, 1885, 2125
ArsI	GACNNNNNNTTYG	6 cut(s)	101, 133, 851, 880, 883, 912
AseI	ATTAAT	2 cut(s)	702, 1236
Asp700I	GAANNNNTTC	1 cut(s)	1036
AspLEI	GCGC	4 cut(s)	53, 152, 442, 2286
AspS9I	GGNCC	4 cut(s)	1043, 1063, 1151, 1786
AsuC2I	CCSGG	3 cut(s)	143, 204, 1931
AsuII	TTCGAA	2 cut(s)	1030, 1187
AvaII	GGWCC	1 cut(s)	1786
BaeGI	GKGCMC	1 cut(s)	504
BanI	GGYRCC	1 cut(s)	50
BanII	GRGCYC	4 cut(s)	126, 135, 1449, 1931
BarI	GAAGNNNNNNTAC	2 cut(s)	1626, 1658
BbsI	GAAGAC	1 cut(s)	2202
Bbv12I	GWGCWC	4 cut(s)	126, 135, 504, 1449
BbvCI	CCTCAGC	1 cut(s)	2034
BbvI	GCAGC	4 cut(s)	91, 932, 1336, 1364
BceAI	ACGGC	2 cut(s)	384, 1931
BciT130I	CCWGG	3 cut(s)	232, 982, 2049
BclI	TGATCA	1 cut(s)	838
BcnI	CCSGG	3 cut(s)	143, 204, 1931
BcoDI	GTCTC	3 cut(s)	102, 226, 2136
BcuI	ACTAGT	1 cut(s)	1328
BfaI	CTAG	7 cut(s)	360, 381, 818, 878, 891, 1280, 1329
BfmI	CTRYAG	3 cut(s)	1350, 1921, 2262
BfoI	RGCGCY	3 cut(s)	54, 153, 2287
BfrI	CTTAAG	2 cut(s)	494, 740
BfuAI	ACCTGC	1 cut(s)	778
BlpI	GCTNAGC	1 cut(s)	1603
BmcAI	AGTACT	1 cut(s)	1893
Bme1390I	CCNGG	6 cut(s)	143, 204, 232, 982, 1931, 2049
Bme18I	GGWCC	1 cut(s)	1786
BmgT120I	GGNCC	4 cut(s)	1043, 1063, 1151, 1786
BmiI	GGNNCC	5 cut(s)	52, 270, 1044, 1787, 1928
BmrFI	CCNGG	6 cut(s)	143, 204, 232, 982, 1931, 2049
BmrI	ACTGGG	1 cut(s)	102
BmsI	GCATC	2 cut(s)	1755, 1933
BmuI	ACTGGG	1 cut(s)	102
BoxI	GACNNNNGTC	1 cut(s)	1416
BpiI	GAAGAC	1 cut(s)	2202
BplI	GAGNNNNNCTC	2 cut(s)	2017, 2049
Bpu10I	CCTNAGC	1 cut(s)	2034
Bpu1102I	GCTNAGC	1 cut(s)	1603
Bpu14I	TTCGAA	2 cut(s)	1030, 1187
BpuEI	CTTGAG	2 cut(s)	566, 2211
BpuMI	CCSGG	3 cut(s)	143, 204, 1931
Bsa29I	ATCGAT	1 cut(s)	1007
BsaBI	GATNNNNATC	2 cut(s)	704, 1628
BsaHI	GRCGYC	4 cut(s)	30, 51, 419, 2044
BsaI	GGTCTC	1 cut(s)	102
BsaJI	CCNNGG	3 cut(s)	366, 413, 2048
BsaXI	ACNNNNNCTCC	4 cut(s)	82, 112, 634, 664
Bse118I	RCCGGY	4 cut(s)	146, 271, 436, 1101
Bse1I	ACTGG	4 cut(s)	97, 1677, 1704, 2045
Bse8I	GATNNNNATC	2 cut(s)	704, 1628
BseBI	CCWGG	3 cut(s)	232, 982, 2049
BseCI	ATCGAT	1 cut(s)	1007
BseDI	CCNNGG	3 cut(s)	366, 413, 2048
BseGI	GGATG	1 cut(s)	469
BseJI	GATNNNNATC	2 cut(s)	704, 1628
BseMII	CTCAG	4 cut(s)	148, 326, 1617, 2048
BseNI	ACTGG	4 cut(s)	97, 1677, 1704, 2045
BseRI	GAGGAG	9 cut(s)	90, 300, 467, 470, 473, 485, 793, 1313, 1598
BseSI	GKGCMC	1 cut(s)	504
BseX3I	CGGCCG	1 cut(s)	144
BseXI	GCAGC	4 cut(s)	91, 932, 1336, 1364
BsgI	GTGCAG	1 cut(s)	806
Bsh1236I	CGCG	3 cut(s)	196, 219, 442
Bsh1285I	CGRYCG	1 cut(s)	147
BshFI	GGCC	5 cut(s)	146, 1045, 1065, 1101, 1153
BshNI	GGYRCC	1 cut(s)	50
BshVI	ATCGAT	1 cut(s)	1007
BsiEI	CGRYCG	1 cut(s)	147
BsiHKAI	GWGCWC	4 cut(s)	126, 135, 504, 1449
BsiSI	CCGG	9 cut(s)	48, 143, 147, 161, 204, 272, 437, 1102, 1931
BslFI	GGGAC	1 cut(s)	1799
BsmAI	GTCTC	3 cut(s)	102, 226, 2136
BsmBI	CGTCTC	1 cut(s)	226
BsmFI	GGGAC	1 cut(s)	1799
BsmI	GAATGC	1 cut(s)	239
BsnI	GGCC	5 cut(s)	146, 1045, 1065, 1101, 1153
Bso31I	GGTCTC	1 cut(s)	102
Bsp119I	TTCGAA	2 cut(s)	1030, 1187
Bsp1286I	GDGCHC	5 cut(s)	126, 135, 504, 1449, 1931
Bsp1407I	TGTACA	1 cut(s)	477
Bsp143I	GATC	7 cut(s)	165, 395, 720, 838, 991, 1623, 1815
Bsp1720I	GCTNAGC	1 cut(s)	1603
BspACI	CCGC	8 cut(s)	77, 180, 183, 191, 194, 217, 422, 2063
BspANI	GGCC	5 cut(s)	146, 1045, 1065, 1101, 1153
BspCNI	CTCAG	4 cut(s)	147, 325, 1616, 2047
BspDI	ATCGAT	1 cut(s)	1007
BspFNI	CGCG	3 cut(s)	196, 219, 442
BspLI	GGNNCC	5 cut(s)	52, 270, 1044, 1787, 1928
BspMAI	CTGCAG	1 cut(s)	1354
BspMI	ACCTGC	1 cut(s)	778
BspPI	GGATC	1 cut(s)	1618
BspT104I	TTCGAA	2 cut(s)	1030, 1187
BspT107I	GGYRCC	1 cut(s)	50
BspTI	CTTAAG	2 cut(s)	494, 740
BspTNI	GGTCTC	1 cut(s)	102
BsrFI	RCCGGY	4 cut(s)	146, 271, 436, 1101
BsrGI	TGTACA	1 cut(s)	477
BsrI	ACTGG	4 cut(s)	97, 1677, 1704, 2045
BssAI	RCCGGY	4 cut(s)	146, 271, 436, 1101
BssECI	CCNNGG	3 cut(s)	366, 413, 2048
BssMI	GATC	7 cut(s)	165, 395, 720, 838, 991, 1623, 1815
BssNAI	GTATAC	1 cut(s)	1876
BssNI	GRCGYC	4 cut(s)	30, 51, 419, 2044
Bst1107I	GTATAC	1 cut(s)	1876
Bst2UI	CCWGG	3 cut(s)	232, 982, 2049
Bst4CI	ACNGT	4 cut(s)	598, 1735, 1790, 1812
Bst6I	CTCTTC	5 cut(s)	444, 503, 770, 845, 1818
BstACI	GRCGYC	4 cut(s)	30, 51, 419, 2044
BstAFI	CTTAAG	2 cut(s)	494, 740
BstAPI	GCANNNNNTGC	1 cut(s)	1349
BstAUI	TGTACA	1 cut(s)	477
BstBI	TTCGAA	2 cut(s)	1030, 1187
BstC8I	GCNNGC	5 cut(s)	148, 209, 228, 589, 2030
BstDEI	CTNAG	6 cut(s)	134, 312, 870, 1603, 2034, 2173
BstDSI	CCRYGG	1 cut(s)	366
BstENI	CCTNNNNNAGG	1 cut(s)	384
BstF5I	GGATG	1 cut(s)	469
BstFNI	CGCG	3 cut(s)	196, 219, 442
BstH2I	RGCGCY	3 cut(s)	54, 153, 2287
BstHHI	GCGC	4 cut(s)	53, 152, 442, 2286
BstKTI	GATC	7 cut(s)	168, 398, 723, 841, 994, 1626, 1818
BstMAI	GTCTC	3 cut(s)	102, 226, 2136
BstMBI	GATC	7 cut(s)	165, 395, 720, 838, 991, 1623, 1815
BstMCI	CGRYCG	1 cut(s)	147
BstMWI	GCNNNNNNNGC	8 cut(s)	130, 179, 213, 236, 418, 1349, 1904, 2050
BstNI	CCWGG	3 cut(s)	232, 982, 2049
BstPAI	GACNNNNGTC	1 cut(s)	1416
BstSCI	CCNGG	6 cut(s)	141, 202, 230, 980, 1929, 2047
BstSFI	CTRYAG	3 cut(s)	1350, 1921, 2262
BstSLI	GKGCMC	1 cut(s)	504
BstUI	CGCG	3 cut(s)	196, 219, 442
BstV1I	GCAGC	4 cut(s)	91, 932, 1336, 1364
BstV2I	GAAGAC	1 cut(s)	2202
BstXI	CCANNNNNNTGG	5 cut(s)	512, 1073, 1672, 2138, 2280
BstZ17I	GTATAC	1 cut(s)	1876
BstZI	CGGCCG	1 cut(s)	144
Bsu15I	ATCGAT	1 cut(s)	1007
BsuRI	GGCC	5 cut(s)	146, 1045, 1065, 1101, 1153
BsuTUI	ATCGAT	1 cut(s)	1007
BtgI	CCRYGG	1 cut(s)	366
BtsCI	GGATG	1 cut(s)	469
BtsI	GCAGTG	1 cut(s)	1722
BtsIMutI	CAGTG	2 cut(s)	1722, 2038
BveI	ACCTGC	1 cut(s)	778
Cac8I	GCNNGC	5 cut(s)	148, 209, 228, 589, 2030
CfoI	GCGC	4 cut(s)	53, 152, 442, 2286
Cfr10I	RCCGGY	4 cut(s)	146, 271, 436, 1101
Cfr13I	GGNCC	4 cut(s)	1043, 1063, 1151, 1786
ClaI	ATCGAT	1 cut(s)	1007
CseI	GACGC	3 cut(s)	208, 427, 2033
CsiI	ACCWGGT	1 cut(s)	980
Csp6I	GTAC	7 cut(s)	432, 478, 1396, 1613, 1808, 1892, 2017
CviAII	CATG	5 cut(s)	67, 822, 842, 1996, 2280
CviQI	GTAC	7 cut(s)	432, 478, 1396, 1613, 1808, 1892, 2017
DdeI	CTNAG	6 cut(s)	134, 312, 870, 1603, 2034, 2173
DinI	GGCGCC	1 cut(s)	52
DpnI	GATC	7 cut(s)	167, 397, 722, 840, 993, 1625, 1817
DpnII	GATC	7 cut(s)	165, 395, 720, 838, 991, 1623, 1815
DraI	TTTAAA	1 cut(s)	1506
EaeI	YGGCCR	2 cut(s)	144, 1099
EagI	CGGCCG	1 cut(s)	144
Eam1104I	CTCTTC	5 cut(s)	444, 503, 770, 845, 1818
EarI	CTCTTC	5 cut(s)	444, 503, 770, 845, 1818
Ecl136II	GAGCTC	3 cut(s)	124, 133, 1447
EclXI	CGGCCG	1 cut(s)	144
Eco24I	GRGCYC	4 cut(s)	126, 135, 1449, 1931
Eco31I	GGTCTC	1 cut(s)	102
Eco47I	GGWCC	1 cut(s)	1786
Eco52I	CGGCCG	1 cut(s)	144
Eco53kI	GAGCTC	3 cut(s)	124, 133, 1447
Eco57I	CTGAAG	2 cut(s)	321, 2201
EcoICRI	GAGCTC	3 cut(s)	124, 133, 1447
EcoNI	CCTNNNNNAGG	1 cut(s)	384
EcoO109I	RGGNCCY	1 cut(s)	1043
EcoRI	GAATTC	1 cut(s)	198
EcoRII	CCWGG	3 cut(s)	230, 980, 2047
EcoT38I	GRGCYC	4 cut(s)	126, 135, 1449, 1931
EgeI	GGCGCC	1 cut(s)	52
EheI	GGCGCC	1 cut(s)	52
Esp3I	CGTCTC	1 cut(s)	226
FaeI	CATG	5 cut(s)	70, 825, 845, 1999, 2283
FaqI	GGGAC	1 cut(s)	1799
FatI	CATG	5 cut(s)	66, 821, 841, 1995, 2279
FauNDI	CATATG	1 cut(s)	926
FbaI	TGATCA	1 cut(s)	838
FblI	GTMKAC	2 cut(s)	1262, 1875
FokI	GGATG	1 cut(s)	476
FriOI	GRGCYC	4 cut(s)	126, 135, 1449, 1931
FspBI	CTAG	7 cut(s)	360, 381, 818, 878, 891, 1280, 1329
GlaI	GCGC	4 cut(s)	52, 151, 441, 2285
HaeII	RGCGCY	3 cut(s)	54, 153, 2287
HaeIII	GGCC	5 cut(s)	146, 1045, 1065, 1101, 1153
HapII	CCGG	9 cut(s)	48, 143, 147, 161, 204, 272, 437, 1102, 1931
HgaI	GACGC	3 cut(s)	208, 427, 2033
HhaI	GCGC	4 cut(s)	53, 152, 442, 2286
Hin1I	GRCGYC	4 cut(s)	30, 51, 419, 2044
Hin1II	CATG	5 cut(s)	70, 825, 845, 1999, 2283
Hin6I	GCGC	4 cut(s)	51, 150, 440, 2284
HinP1I	GCGC	4 cut(s)	51, 150, 440, 2284
HincII	GTYRAC	1 cut(s)	2086
HindII	GTYRAC	1 cut(s)	2086
HindIII	AAGCTT	1 cut(s)	1181
HinfI	GANTC	6 cut(s)	59, 110, 138, 811, 874, 1004
HpaI	GTTAAC	1 cut(s)	2086
HpaII	CCGG	9 cut(s)	48, 143, 147, 161, 204, 272, 437, 1102, 1931
Hpy166II	GTNNAC	9 cut(s)	350, 434, 478, 502, 978, 1263, 1528, 1876, 2086
Hpy188I	TCNGA	4 cut(s)	137, 315, 996, 1647
Hpy188III	TCNNGA	9 cut(s)	87, 266, 718, 1254, 1369, 1426, 1852, 2131, 2228
Hpy8I	GTNNAC	9 cut(s)	350, 434, 478, 502, 978, 1263, 1528, 1876, 2086
Hpy99I	CGWCG	4 cut(s)	32, 35, 38, 2160
HpyCH4III	ACNGT	4 cut(s)	598, 1735, 1790, 1812
HpyCH4IV	ACGT	4 cut(s)	30, 866, 1173, 2015
HpyCH4V	TGCA	6 cut(s)	40, 329, 502, 787, 1352, 1768
HpyF10VI	GCNNNNNNNGC	8 cut(s)	130, 179, 213, 236, 418, 1349, 1904, 2050
HpyF3I	CTNAG	6 cut(s)	134, 312, 870, 1603, 2034, 2173
HpySE526I	ACGT	4 cut(s)	30, 866, 1173, 2015
Hsp92I	GRCGYC	4 cut(s)	30, 51, 419, 2044
Hsp92II	CATG	5 cut(s)	70, 825, 845, 1999, 2283
HspAI	GCGC	4 cut(s)	51, 150, 440, 2284
KasI	GGCGCC	1 cut(s)	50
KroI	GCCGGC	1 cut(s)	146
KroNI	GCCGGC	1 cut(s)	148
Ksp22I	TGATCA	1 cut(s)	838
KspAI	GTTAAC	1 cut(s)	2086
Kzo9I	GATC	7 cut(s)	165, 395, 720, 838, 991, 1623, 1815
LmnI	GCTCC	4 cut(s)	268, 917, 1472, 1926
Lsp1109I	GCAGC	4 cut(s)	91, 932, 1336, 1364
LweI	GCATC	2 cut(s)	1755, 1933
MabI	ACCWGGT	1 cut(s)	980
MaeI	CTAG	7 cut(s)	360, 381, 818, 878, 891, 1280, 1329
MaeII	ACGT	4 cut(s)	30, 866, 1173, 2015
MaeIII	GTNAC	8 cut(s)	295, 333, 540, 553, 685, 886, 1638, 1718
MalI	GATC	7 cut(s)	167, 397, 722, 840, 993, 1625, 1817
MboI	GATC	7 cut(s)	165, 395, 720, 838, 991, 1623, 1815
MfeI	CAATTG	1 cut(s)	1977
MhlI	GDGCHC	5 cut(s)	126, 135, 504, 1449, 1931
Mly113I	GGCGCC	1 cut(s)	51
MlyI	GAGTC	2 cut(s)	53, 104
MmeI	TCCRAC	1 cut(s)	138
MroNI	GCCGGC	1 cut(s)	146
MroXI	GAANNNNTTC	1 cut(s)	1036
MslI	CAYNNNNRTG	2 cut(s)	1020, 2278
MspA1I	CMGCKG	1 cut(s)	424
MspCI	CTTAAG	2 cut(s)	494, 740
MspI	CCGG	9 cut(s)	48, 143, 147, 161, 204, 272, 437, 1102, 1931
MspR9I	CCNGG	6 cut(s)	143, 204, 232, 982, 1931, 2049
MunI	CAATTG	1 cut(s)	1977
Mva1269I	GAATGC	1 cut(s)	239
MvaI	CCWGG	3 cut(s)	232, 982, 2049
MvnI	CGCG	3 cut(s)	196, 219, 442
MwoI	GCNNNNNNNGC	8 cut(s)	130, 179, 213, 236, 418, 1349, 1904, 2050
NaeI	GCCGGC	1 cut(s)	148
NarI	GGCGCC	1 cut(s)	51
NciI	CCSGG	3 cut(s)	143, 204, 1931
NdeI	CATATG	1 cut(s)	926
NdeII	GATC	7 cut(s)	165, 395, 720, 838, 991, 1623, 1815
NgoMIV	GCCGGC	1 cut(s)	146
NlaIII	CATG	5 cut(s)	70, 825, 845, 1999, 2283
NlaIV	GGNNCC	5 cut(s)	52, 270, 1044, 1787, 1928
NmeAIII	GCCGAG	1 cut(s)	438
NmuCI	GTSAC	4 cut(s)	295, 333, 685, 886
NspV	TTCGAA	2 cut(s)	1030, 1187
PaqCI	CACCTGC	1 cut(s)	778
PcsI	WCGNNNNNNNCGW	3 cut(s)	33, 863, 1290
PctI	GAATGC	1 cut(s)	239
PdiI	GCCGGC	1 cut(s)	148
PdmI	GAANNNNTTC	1 cut(s)	1036
PfeI	GAWTC	4 cut(s)	138, 811, 874, 1004
PflFI	GACNNNGTC	1 cut(s)	32
PleI	GAGTC	2 cut(s)	53, 104
PluTI	GGCGCC	1 cut(s)	54
PpsI	GAGTC	2 cut(s)	53, 104
PshAI	GACNNNNGTC	1 cut(s)	1416
PshBI	ATTAAT	2 cut(s)	702, 1236
Psp124BI	GAGCTC	3 cut(s)	126, 135, 1449
Psp6I	CCWGG	3 cut(s)	230, 980, 2047
PspGI	CCWGG	3 cut(s)	230, 980, 2047
PspN4I	GGNNCC	5 cut(s)	52, 270, 1044, 1787, 1928
PspPI	GGNCC	4 cut(s)	1043, 1063, 1151, 1786
PsrI	GAACNNNNNNTAC	2 cut(s)	355, 387
PstI	CTGCAG	1 cut(s)	1354
PsyI	GACNNNGTC	1 cut(s)	32
RsaI	GTAC	7 cut(s)	433, 479, 1397, 1614, 1809, 1893, 2018
RsaNI	GTAC	7 cut(s)	432, 478, 1396, 1613, 1808, 1892, 2017
RseI	CAYNNNNRTG	2 cut(s)	1020, 2278
SacI	GAGCTC	3 cut(s)	126, 135, 1449
Sau3AI	GATC	7 cut(s)	165, 395, 720, 838, 991, 1623, 1815
Sau96I	GGNCC	4 cut(s)	1043, 1063, 1151, 1786
ScaI	AGTACT	1 cut(s)	1893
SchI	GAGTC	2 cut(s)	53, 104
ScrFI	CCNGG	6 cut(s)	143, 204, 232, 982, 1931, 2049
SduI	GDGCHC	5 cut(s)	126, 135, 504, 1449, 1931
SexAI	ACCWGGT	1 cut(s)	980
SfaNI	GCATC	2 cut(s)	1755, 1933
SfcI	CTRYAG	3 cut(s)	1350, 1921, 2262
SfoI	GGCGCC	1 cut(s)	52
SfuI	TTCGAA	2 cut(s)	1030, 1187
SgrAI	CRCCGGYG	1 cut(s)	436
SinI	GGWCC	1 cut(s)	1786
SmiMI	CAYNNNNRTG	2 cut(s)	1020, 2278
SmlI	CTYRAG	4 cut(s)	494, 545, 740, 2226
SmoI	CTYRAG	4 cut(s)	494, 545, 740, 2226
SpeI	ACTAGT	1 cut(s)	1328
SsiI	CCGC	8 cut(s)	77, 180, 183, 191, 194, 217, 422, 2063
SspDI	GGCGCC	1 cut(s)	50
SspI	AATATT	1 cut(s)	2190
SspMI	CTAG	7 cut(s)	360, 381, 818, 878, 891, 1280, 1329
SstI	GAGCTC	3 cut(s)	126, 135, 1449
StyD4I	CCNGG	6 cut(s)	141, 202, 230, 980, 1929, 2047
TaaI	ACNGT	4 cut(s)	598, 1735, 1790, 1812
TaiI	ACGT	4 cut(s)	33, 869, 1176, 2018
TatI	WGTACW	5 cut(s)	477, 1395, 1612, 1807, 1891
TauI	GCSGC	6 cut(s)	79, 185, 193, 196, 219, 424
TfiI	GAWTC	4 cut(s)	138, 811, 874, 1004
TscAI	CASTG	2 cut(s)	1729, 2045
TseFI	GTSAC	4 cut(s)	295, 333, 685, 886
TseI	GCWGC	4 cut(s)	79, 920, 1349, 1352
Tsp45I	GTSAC	4 cut(s)	295, 333, 685, 886
TspDTI	ATGAA	7 cut(s)	55, 300, 1004, 1208, 1238, 1844, 2012
TspRI	CASTG	2 cut(s)	1729, 2045
Tth111I	GACNNNGTC	1 cut(s)	32
Vha464I	CTTAAG	2 cut(s)	494, 740
VneI	GTGCAC	1 cut(s)	500
VpaK11BI	GGWCC	1 cut(s)	1786
VspI	ATTAAT	2 cut(s)	702, 1236
XagI	CCTNNNNNAGG	1 cut(s)	384
XapI	RAATTY	7 cut(s)	42, 198, 940, 1082, 1206, 1885, 2125
XmiI	GTMKAC	2 cut(s)	1262, 1875
XmnI	GAANNNNTTC	1 cut(s)	1036
XspI	CTAG	7 cut(s)	360, 381, 818, 878, 891, 1280, 1329
ZraI	GACGTC	1 cut(s)	31
ZrmI	AGTACT	1 cut(s)	1893

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.