Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003680

Physical Location & Seq

Genomic Coordinates Forward (+)

6815049 .. 6818657

Gene Structure 3609 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1087000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2283 bp

ATGGAAGGTGGAAAAGGCGTGGGGGAGAAGAAAGGAACAAACGACGTGGTTTTGCAGATTTCGGTCGGAGAAGAACACACGAGATACAGTAACGAAATCAGAGACTCGAATCTCCAGCTGACAGAGCTTCAGAGCCTCAGGGTCTCCGGCTCTGCTTCTCCAGAAATCCCAAAGCCGAGTCCCTCGCCGCCGCCGGGGAAGCCGCCGAAAATCCCAGCCGAACCCACTTCCCGGCGAGCTTCGTTTTCCCGGTCCGCGTTCTCGAAGCCCAAGTCGAGGTTCGTGGAGCCTGTTCCTCCTGCTGAAAAGAAGGCGACCGACGAAATTCCCCAGTTGAAGTCAACTGCTAATTCCCCGAATGTGGCATCGCCAAGCTCCAAAATCGCAGCCACAACACCGAGAGACGCTCTGAGATCAGCCCCGATAACCCCACGAACGCCGTTGATCAACAGCAGCGAGGAGGACGACGATGAGGAGGTTTACAAGACTGCAAAGCTTAAGGTGCGTGAGAAGTCTGGCAAGAAGATGAAAAAGTTAGCAGTAATTGAGTTGATTGCATTTGTTTGTATTGTTGGGTTTTTTATTGCCTGCTTAACTGTTCCTAAGTTGGAAAAGAAGATGTATTGGGGATTGGAATTGTGGAAATGGTCTGTCTTGGTGTTGGTTGTTTTGTGTGGTAGATTGGTCACAGAATGGTTTATCAATGTTCTGGTTTTCGTGATTGAGTTGAACTTTTTGCTTAAGAAGAATGTTCTGTATTTCGTTTACGGGTTGAAGGGAAGCGTACAGATTTTTATTTGGTTGAGTTTGGTTCTTCTGGCATGGGGTTTGTTGTTCAACCGCGGAGTGAAAAGGTCAAGGAAAACCTCTAGGATTCTAGGTTACGTTACAAGGGGTCTGGCTTCGTGTCTAATTGGAGCGGGCATATGGCTGGTGAAGAATTTATTTGTCAAAGTGGTAGCTTCTTCGTTCCAATGCAATAGATTCTTTGATCGGATTCAAGAATCAATTTTTCATCAGTATGTTCTCCGCACCCTTTCCGGGCCTCCTCTGATGGAAATGGCAGAAAGAGTCGGGAAGACCCCAAGTAGGGGCCAGTTGAGCTTCAAAAATTTGAAGGGAGGGAAAAAGGAGGGGAAGGAAGGGCCCAAAGAAGAGGTGATTGATGTAGAAAAGCTAAAAAAGATGAACCAGAAGAAAATTTCTGCTTGGACCTTGAAAGGGTTGATTAACATGGTAAGGAGTTCCGGGCTATCTACCATCTCCGACACACTTGAGAGTCTTGACGATGAGGAGAGTGAGCAGAAAGGTAAGGAGATTACTAGTGAGTGGGAAGCAAAGGCTGTGGCTTATGATATTTTCCTGAATGTCGCCAAGCATAGCAACAAGTACATTGAGGAAGATGACCTCTTGCGCTTCATGAAAAAGGAGGAGGTCGACAATGTATTACCTCTGTTTGAAGGGGCAGCAGAAACGGGCAAGATCAAGAGAAAAGCTTTGAAGAACTGGCTGGTGAATGTCTACCTTGAACGCAAATCTCTGGCACATTCGTTAAATGACACTAAAACAGCGATAGAGGAGCTGAATAGGCTTGCCTCAGGGGTATTGCTTCTTGTGATCATTATCGTGTGGCTACTTCTGATGGGATTTTTGACAACCAACATACTGGTCTTTATATCATCTCAGCTCTTACTGGTGGTCTTTGTATTTGGTAATACTGCTAAAACTGTGTTTGAAGCAATCATTTTCGTCTTTGTGATGCACCCTTTTGATGTAGGGGACCGTTGTGTCGTTGATGGAGTACAGCTCATAGTTGAAGAGATGAACATATTGACAACGATCTTCCTAAGATATGACAACGAGAAAATTTTCTATCCAAATTCAGTACTGGCTTCTAAACCCATCAGCAACTTCTACAGGAGTCCGGAGATGGGTGATTCTGTTGAATTTGCTGTTCATGTTTCTACAACGGCTGATACTATTGTGGCTCTAAAAGCCAGAATAAAAGAGTACTTGGACAGCAAGAGTCAGCACTGGCGTCCTGCCCATAGCGTGGTGGTTAAGGATATTGAGGACGTCAACAAAATGAAAATGGCTCTTTATGTTACGCATACCATAAACTTTCAGAACTACGGAGACAAGAGCAGTCGGAGATCAGATTTGGTCTTAGAGCTGAAGAAAATATTTGAAGATCTTGGTATAAAATATAAACTCCTGCCTCAAGAAGTTCATCTCCGCTATGTCGGATCATCCACTGCAGAACTTCCACCAACGTGGCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel PsbX

Protein Analysis

761

Amino Acids

85.56

Weight (kDa)

9.3

Isoelectric Point (pI)

42.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Msy1	PF25886	184 - 324	9.4e-06	Mechanosensitive ion channel protein Msy1-like, transmembrane domain
MS_channel_2nd	PF00924	582 - 638	1.7e-09	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003680)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g08260 FvH4_1g08260 FvH4_1g08260 FvH4_1g08260
malus_domestica	MD02G1086900.v1.1 MD02G1087000.v1.1 MD15G1214500.v1.1 MD15G1214600.v1.1
prunus_persica	Prupe.7G202700_v2.0.a1
pyrus_communis	pycom02g06990 pycom02g07000 pycom15g19040
rosa_chinensis	RchiOBHm_Chr2g0094331
rosa_laevigata	RLG00000016480
rosa_multiflora	Rmu_sc0003819.1_g000016 Rmu_sc0025410.1_g000001 Rmu_sc0025411.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147230 Rroxscaffold_7G00185070
rosa_rugosa	Rorug02G0043300 Rorug02G0043300
rosa_samantha	Rh2AG091000 Rh2AG091100 Rh2BG090900 Rh2CG093400 Rh2CG093500 Rh2DG089900 Rh6AG505100 Rh6BG272500 Rh6CG273600 Rh6CG521500 Rh6CG521800 Rh6DG266600 Rh6DG507600
rosa_wichuraiana	Rw6G043950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1787
AatII	GACGTC	1 cut(s)	2079
AccB7I	CCANNNNNTGG	1 cut(s)	2053
AccBSI	CCGCTC	1 cut(s)	920
AccI	GTMKAC	2 cut(s)	1437, 1521
AccII	CGCG	2 cut(s)	257, 843
AccIII	TCCGGA	1 cut(s)	1924
AciI	CCGC	9 cut(s)	188, 191, 203, 255, 841, 843, 920, 1030, 2236
AclWI	GGATC	1 cut(s)	2254
AcsI	RAATTY	7 cut(s)	324, 940, 1111, 1200, 1866, 1879, 1946
AcuI	CTGAAG	2 cut(s)	113, 2195
AcyI	GRCGYC	2 cut(s)	2038, 2076
AfaI	GTAC	5 cut(s)	786, 1391, 1803, 1887, 2012
AflII	CTTAAG	2 cut(s)	497, 740
AhlI	ACTAGT	1 cut(s)	1322
AjiI	CACGTC	1 cut(s)	46
AjuI	GAANNNNNNNTTGG	2 cut(s)	263, 295
AleI	CACNNNNGTG	1 cut(s)	2272
Alw26I	GTCTC	4 cut(s)	96, 148, 396, 2130
AlwI	GGATC	1 cut(s)	2254
Aor13HI	TCCGGA	1 cut(s)	1924
AoxI	GGCC	3 cut(s)	1043, 1093, 1145
ApaI	GGGCCC	1 cut(s)	1149
ApeKI	GCWGC	3 cut(s)	386, 453, 1466
ApoI	RAATTY	7 cut(s)	324, 940, 1111, 1200, 1866, 1879, 1946
ArsI	GACNNNNNNTTYG	2 cut(s)	257, 289
Asp700I	GAANNNNTTC	1 cut(s)	1868
AspLEI	GCGC	1 cut(s)	1416
AspS9I	GGNCC	7 cut(s)	252, 1043, 1093, 1145, 1146, 1212, 1780
AsuC2I	CCSGG	5 cut(s)	195, 232, 250, 1042, 1249
AsuHPI	GGTGA	4 cut(s)	946, 1171, 1525, 1946
AvaII	GGWCC	3 cut(s)	252, 1212, 1780
AxyI	CCTNAGG	2 cut(s)	137, 1597
BaeGI	GKGCMC	1 cut(s)	1149
BaeI	ACNNNNGTAYC	2 cut(s)	1968, 2001
BanII	GRGCYC	1 cut(s)	1149
BauI	CACGAG	1 cut(s)	79
BbsI	GAAGAC	1 cut(s)	1085
BbvI	GCAGC	3 cut(s)	398, 465, 1478
BccI	CCATC	6 cut(s)	1048, 1268, 1636, 1790, 1910, 1924
BceAI	ACGGC	2 cut(s)	424, 1986
BclI	TGATCA	2 cut(s)	444, 1617
BcnI	CCSGG	5 cut(s)	195, 232, 250, 1042, 1249
BcoDI	GTCTC	4 cut(s)	96, 148, 396, 2130
BcuI	ACTAGT	1 cut(s)	1322
BfaI	CTAG	3 cut(s)	870, 878, 1323
BfmI	CTRYAG	2 cut(s)	1915, 2256
BfrI	CTTAAG	2 cut(s)	497, 740
BglII	AGATCT	1 cut(s)	2191
BisI	GCNGC	6 cut(s)	188, 191, 203, 387, 454, 1467
BlsI	GCNGC	6 cut(s)	189, 192, 204, 388, 455, 1468
BmcAI	AGTACT	2 cut(s)	1887, 2012
Bme1390I	CCNGG	5 cut(s)	195, 232, 250, 1042, 1249
Bme18I	GGWCC	3 cut(s)	252, 1212, 1780
BmgBI	CACGTC	1 cut(s)	46
BmgT120I	GGNCC	7 cut(s)	252, 1043, 1093, 1145, 1146, 1212, 1780
BmiI	GGNNCC	4 cut(s)	288, 1094, 1147, 1781
BmrFI	CCNGG	5 cut(s)	195, 232, 250, 1042, 1249
BmrI	ACTGGG	1 cut(s)	325
BmsI	GCATC	2 cut(s)	374, 1749
BmuI	ACTGGG	1 cut(s)	325
BpiI	GAAGAC	1 cut(s)	1085
BplI	GAGNNNNNCTC	4 cut(s)	391, 423, 1791, 1823
BpmI	CTGGAG	2 cut(s)	98, 144
BpuEI	CTTGAG	2 cut(s)	1295, 2205
BpuMI	CCSGG	5 cut(s)	195, 232, 250, 1042, 1249
BsaBI	GATNNNNATC	1 cut(s)	1622
BsaHI	GRCGYC	2 cut(s)	2038, 2076
BsaI	GGTCTC	1 cut(s)	148
BsaJI	CCNNGG	2 cut(s)	194, 841
BsaWI	WCCGGW	1 cut(s)	1924
Bse1I	ACTGG	7 cut(s)	331, 1096, 1511, 1671, 1698, 1893, 2039
Bse21I	CCTNAGG	2 cut(s)	137, 1597
Bse8I	GATNNNNATC	1 cut(s)	1622
BseAI	TCCGGA	1 cut(s)	1924
BseDI	CCNNGG	2 cut(s)	194, 841
BseGI	GGATG	1 cut(s)	2249
BseJI	GATNNNNATC	1 cut(s)	1622
BseMII	CTCAG	4 cut(s)	151, 401, 1611, 1697
BseNI	ACTGG	7 cut(s)	331, 1096, 1511, 1671, 1698, 1893, 2039
BseRI	GAGGAG	6 cut(s)	473, 488, 1038, 1307, 1445, 1592
BseSI	GKGCMC	1 cut(s)	1149
BseXI	GCAGC	3 cut(s)	398, 465, 1478
BseYI	CCCAGC	1 cut(s)	214
Bsh1236I	CGCG	2 cut(s)	257, 843
Bsh1285I	CGRYCG	2 cut(s)	66, 318
BshFI	GGCC	3 cut(s)	1045, 1095, 1147
BsiEI	CGRYCG	2 cut(s)	66, 318
BsiSI	CCGG	7 cut(s)	147, 194, 232, 250, 1041, 1248, 1925
BslFI	GGGAC	2 cut(s)	165, 1793
BsmAI	GTCTC	4 cut(s)	96, 148, 396, 2130
BsmBI	CGTCTC	1 cut(s)	396
BsmFI	GGGAC	2 cut(s)	165, 1793
BsnI	GGCC	3 cut(s)	1045, 1095, 1147
Bso31I	GGTCTC	1 cut(s)	148
Bsp120I	GGGCCC	1 cut(s)	1145
Bsp1286I	GDGCHC	1 cut(s)	1149
Bsp13I	TCCGGA	1 cut(s)	1924
Bsp143I	GATC	9 cut(s)	413, 444, 991, 1482, 1617, 1839, 2153, 2191, 2246
BspACI	CCGC	9 cut(s)	188, 191, 203, 255, 841, 843, 920, 1030, 2236
BspANI	GGCC	3 cut(s)	1045, 1095, 1147
BspCNI	CTCAG	4 cut(s)	150, 402, 1610, 1696
BspEI	TCCGGA	1 cut(s)	1924
BspFNI	CGCG	2 cut(s)	257, 843
BspHI	TCATGA	1 cut(s)	1419
BspLI	GGNNCC	4 cut(s)	288, 1094, 1147, 1781
BspMAI	CTGCAG	1 cut(s)	2260
BspPI	GGATC	1 cut(s)	2254
BspTI	CTTAAG	2 cut(s)	497, 740
BspTNI	GGTCTC	1 cut(s)	148
BsrBI	CCGCTC	1 cut(s)	920
BsrI	ACTGG	7 cut(s)	331, 1096, 1511, 1671, 1698, 1893, 2039
BssECI	CCNNGG	2 cut(s)	194, 841
BssMI	GATC	9 cut(s)	413, 444, 991, 1482, 1617, 1839, 2153, 2191, 2246
BssNI	GRCGYC	2 cut(s)	2038, 2076
BssSI	CACGAG	1 cut(s)	79
Bst2BI	CACGAG	1 cut(s)	79
Bst4CI	ACNGT	4 cut(s)	89, 598, 1729, 1784
Bst6I	CTCTTC	2 cut(s)	1149, 1812
BstACI	GRCGYC	2 cut(s)	2038, 2076
BstAFI	CTTAAG	2 cut(s)	497, 740
BstC8I	GCNNGC	4 cut(s)	237, 589, 922, 1593
BstDEI	CTNAG	7 cut(s)	137, 410, 603, 1597, 1683, 1847, 2167
BstDSI	CCRYGG	1 cut(s)	841
BstF5I	GGATG	1 cut(s)	2249
BstFNI	CGCG	2 cut(s)	257, 843
BstHHI	GCGC	1 cut(s)	1416
BstKTI	GATC	9 cut(s)	416, 447, 994, 1485, 1620, 1842, 2156, 2194, 2249
BstMAI	GTCTC	4 cut(s)	96, 148, 396, 2130
BstMBI	GATC	9 cut(s)	413, 444, 991, 1482, 1617, 1839, 2153, 2191, 2246
BstMCI	CGRYCG	2 cut(s)	66, 318
BstMWI	GCNNNNNNNGC	6 cut(s)	124, 199, 502, 1101, 1588, 1994
BstSCI	CCNGG	5 cut(s)	193, 230, 248, 1040, 1247
BstSFI	CTRYAG	2 cut(s)	1915, 2256
BstSLI	GKGCMC	1 cut(s)	1149
BstUI	CGCG	2 cut(s)	257, 843
BstV1I	GCAGC	3 cut(s)	398, 465, 1478
BstV2I	GAAGAC	1 cut(s)	1085
BstX2I	RGATCY	1 cut(s)	2191
BstXI	CCANNNNNNTGG	2 cut(s)	1666, 2274
BstYI	RGATCY	1 cut(s)	2191
Bsu36I	CCTNAGG	2 cut(s)	137, 1597
BsuRI	GGCC	3 cut(s)	1045, 1095, 1147
BtgI	CCRYGG	1 cut(s)	841
BtgZI	GCGATG	1 cut(s)	351
BtrI	CACGTC	1 cut(s)	46
BtsCI	GGATG	1 cut(s)	2249
BtsI	GCAGTG	1 cut(s)	2253
BtsIMutI	CAGTG	2 cut(s)	2032, 2253
Cac8I	GCNNGC	4 cut(s)	237, 589, 922, 1593
CciI	TCATGA	1 cut(s)	1419
CfoI	GCGC	1 cut(s)	1416
Cfr13I	GGNCC	7 cut(s)	252, 1043, 1093, 1145, 1146, 1212, 1780
Cfr42I	CCGCGG	1 cut(s)	844
CpoI	CGGWCCG	1 cut(s)	252
CseI	GACGC	2 cut(s)	413, 2027
Csp6I	GTAC	5 cut(s)	785, 1390, 1802, 1886, 2011
CspCI	CAANNNNNGTGG	4 cut(s)	27, 62, 1326, 1361
CspI	CGGWCCG	1 cut(s)	252
CviAII	CATG	4 cut(s)	822, 1234, 1420, 1958
CviQI	GTAC	5 cut(s)	785, 1390, 1802, 1886, 2011
DdeI	CTNAG	7 cut(s)	137, 410, 603, 1597, 1683, 1847, 2167
DpnI	GATC	9 cut(s)	415, 446, 993, 1484, 1619, 1841, 2155, 2193, 2248
DpnII	GATC	9 cut(s)	413, 444, 991, 1482, 1617, 1839, 2153, 2191, 2246
DrdI	GACNNNNNNGTC	1 cut(s)	1787
DseDI	GACNNNNNNGTC	1 cut(s)	1787
Eam1104I	CTCTTC	2 cut(s)	1149, 1812
EarI	CTCTTC	2 cut(s)	1149, 1812
Eco24I	GRGCYC	1 cut(s)	1149
Eco31I	GGTCTC	1 cut(s)	148
Eco47I	GGWCC	3 cut(s)	252, 1212, 1780
Eco57I	CTGAAG	2 cut(s)	113, 2195
Eco81I	CCTNAGG	2 cut(s)	137, 1597
EcoO109I	RGGNCCY	1 cut(s)	1145
EcoT38I	GRGCYC	1 cut(s)	1149
Esp3I	CGTCTC	1 cut(s)	396
FaeI	CATG	4 cut(s)	825, 1237, 1423, 1961
FaqI	GGGAC	2 cut(s)	165, 1793
FatI	CATG	4 cut(s)	821, 1233, 1419, 1957
FauI	CCCGC	1 cut(s)	913
FauNDI	CATATG	1 cut(s)	926
FbaI	TGATCA	2 cut(s)	444, 1617
FblI	GTMKAC	2 cut(s)	1437, 1521
Fnu4HI	GCNGC	6 cut(s)	188, 191, 203, 387, 454, 1467
FokI	GGATG	1 cut(s)	2236
FriOI	GRGCYC	1 cut(s)	1149
Fsp4HI	GCNGC	6 cut(s)	188, 191, 203, 387, 454, 1467
FspBI	CTAG	3 cut(s)	870, 878, 1323
GlaI	GCGC	1 cut(s)	1415
GluI	GCNGC	6 cut(s)	188, 191, 203, 387, 454, 1467
GsaI	CCCAGC	1 cut(s)	218
GsuI	CTGGAG	2 cut(s)	98, 144
HaeIII	GGCC	3 cut(s)	1045, 1095, 1147
HapII	CCGG	7 cut(s)	147, 194, 232, 250, 1041, 1248, 1925
HgaI	GACGC	2 cut(s)	413, 2027
HhaI	GCGC	1 cut(s)	1416
Hin1I	GRCGYC	2 cut(s)	2038, 2076
Hin1II	CATG	4 cut(s)	825, 1237, 1423, 1961
Hin6I	GCGC	1 cut(s)	1414
HinP1I	GCGC	1 cut(s)	1414
HincII	GTYRAC	3 cut(s)	342, 1438, 2080
HindII	GTYRAC	3 cut(s)	342, 1438, 2080
HindIII	AAGCTT	2 cut(s)	494, 1494
HpaII	CCGG	7 cut(s)	147, 194, 232, 250, 1041, 1248, 1925
HphI	GGTGA	4 cut(s)	946, 1171, 1525, 1946
Hpy166II	GTNNAC	6 cut(s)	342, 481, 766, 1438, 1522, 2080
Hpy8I	GTNNAC	6 cut(s)	342, 481, 766, 1438, 1522, 2080
Hpy99I	CGWCG	3 cut(s)	47, 323, 470
HpyAV	CCTTC	6 cut(s)	304, 769, 1111, 1132, 1136, 1454
HpyCH4III	ACNGT	4 cut(s)	89, 598, 1729, 1784
HpyCH4IV	ACGT	4 cut(s)	45, 885, 2076, 2273
HpyCH4V	TGCA	6 cut(s)	55, 491, 557, 978, 1762, 2258
HpyF10VI	GCNNNNNNNGC	6 cut(s)	124, 199, 502, 1101, 1588, 1994
HpyF3I	CTNAG	7 cut(s)	137, 410, 603, 1597, 1683, 1847, 2167
HpySE526I	ACGT	4 cut(s)	45, 885, 2076, 2273
Hsp92I	GRCGYC	2 cut(s)	2038, 2076
Hsp92II	CATG	4 cut(s)	825, 1237, 1423, 1961
HspAI	GCGC	1 cut(s)	1414
Kpn2I	TCCGGA	1 cut(s)	1924
Ksp22I	TGATCA	2 cut(s)	444, 1617
KspI	CCGCGG	1 cut(s)	844
Kzo9I	GATC	9 cut(s)	413, 444, 991, 1482, 1617, 1839, 2153, 2191, 2246
LmnI	GCTCC	4 cut(s)	286, 380, 917, 1579
Lsp1109I	GCAGC	3 cut(s)	398, 465, 1478
LweI	GCATC	2 cut(s)	374, 1749
MaeI	CTAG	3 cut(s)	870, 878, 1323
MaeII	ACGT	4 cut(s)	45, 885, 2076, 2273
MaeIII	GTNAC	5 cut(s)	89, 685, 881, 886, 2104
MalI	GATC	9 cut(s)	415, 446, 993, 1484, 1619, 1841, 2155, 2193, 2248
MbiI	CCGCTC	1 cut(s)	920
MboI	GATC	9 cut(s)	413, 444, 991, 1482, 1617, 1839, 2153, 2191, 2246
MflI	RGATCY	1 cut(s)	2191
MhlI	GDGCHC	1 cut(s)	1149
MlyI	GAGTC	6 cut(s)	98, 187, 1080, 1288, 1930, 2035
MmeI	TCCRAC	5 cut(s)	46, 588, 1290, 2129, 2224
MroI	TCCGGA	1 cut(s)	1924
MroXI	GAANNNNTTC	1 cut(s)	1868
MseI	TTAA	6 cut(s)	498, 593, 741, 1230, 1553, 2061
MslI	CAYNNNNRTG	3 cut(s)	1020, 1625, 2272
MspA1I	CMGCKG	2 cut(s)	118, 843
MspCI	CTTAAG	2 cut(s)	497, 740
MspI	CCGG	7 cut(s)	147, 194, 232, 250, 1041, 1248, 1925
MspR9I	CCNGG	5 cut(s)	195, 232, 250, 1042, 1249
MvnI	CGCG	2 cut(s)	257, 843
MwoI	GCNNNNNNNGC	6 cut(s)	124, 199, 502, 1101, 1588, 1994
NciI	CCSGG	5 cut(s)	195, 232, 250, 1042, 1249
NdeI	CATATG	1 cut(s)	926
NdeII	GATC	9 cut(s)	413, 444, 991, 1482, 1617, 1839, 2153, 2191, 2246
NlaIII	CATG	4 cut(s)	825, 1237, 1423, 1961
NlaIV	GGNNCC	4 cut(s)	288, 1094, 1147, 1781
NmeAIII	GCCGAG	1 cut(s)	201
NmuCI	GTSAC	1 cut(s)	685
OliI	CACNNNNGTG	1 cut(s)	2272
PagI	TCATGA	1 cut(s)	1419
PdmI	GAANNNNTTC	1 cut(s)	1868
PfeI	GAWTC	6 cut(s)	109, 874, 984, 997, 1004, 1937
PflMI	CCANNNNNTGG	1 cut(s)	2053
PkrI	GCNGC	6 cut(s)	189, 192, 204, 388, 455, 1468
PleI	GAGTC	6 cut(s)	98, 186, 1079, 1287, 1929, 2034
PpsI	GAGTC	6 cut(s)	98, 186, 1079, 1287, 1929, 2034
PspFI	CCCAGC	1 cut(s)	214
PspN4I	GGNNCC	4 cut(s)	288, 1094, 1147, 1781
PspOMI	GGGCCC	1 cut(s)	1145
PspPI	GGNCC	7 cut(s)	252, 1043, 1093, 1145, 1146, 1212, 1780
PstI	CTGCAG	1 cut(s)	2260
PsuI	RGATCY	1 cut(s)	2191
PvuII	CAGCTG	1 cut(s)	118
RsaI	GTAC	5 cut(s)	786, 1391, 1803, 1887, 2012
RsaNI	GTAC	5 cut(s)	785, 1390, 1802, 1886, 2011
RseI	CAYNNNNRTG	3 cut(s)	1020, 1625, 2272
Rsr2I	CGGWCCG	1 cut(s)	252
RsrII	CGGWCCG	1 cut(s)	252
SacII	CCGCGG	1 cut(s)	844
SalI	GTCGAC	1 cut(s)	1436
SaqAI	TTAA	6 cut(s)	498, 593, 741, 1230, 1553, 2061
SatI	GCNGC	6 cut(s)	188, 191, 203, 387, 454, 1467
Sau3AI	GATC	9 cut(s)	413, 444, 991, 1482, 1617, 1839, 2153, 2191, 2246
Sau96I	GGNCC	7 cut(s)	252, 1043, 1093, 1145, 1146, 1212, 1780
ScaI	AGTACT	2 cut(s)	1887, 2012
SchI	GAGTC	6 cut(s)	98, 187, 1080, 1288, 1930, 2035
ScrFI	CCNGG	5 cut(s)	195, 232, 250, 1042, 1249
SduI	GDGCHC	1 cut(s)	1149
SfaNI	GCATC	2 cut(s)	374, 1749
SfcI	CTRYAG	2 cut(s)	1915, 2256
Sfr303I	CCGCGG	1 cut(s)	844
SgrBI	CCGCGG	1 cut(s)	844
SinI	GGWCC	3 cut(s)	252, 1212, 1780
SmiMI	CAYNNNNRTG	3 cut(s)	1020, 1625, 2272
SmlI	CTYRAG	4 cut(s)	497, 740, 1274, 2220
SmoI	CTYRAG	4 cut(s)	497, 740, 1274, 2220
SpeI	ACTAGT	1 cut(s)	1322
SsiI	CCGC	9 cut(s)	188, 191, 203, 255, 841, 843, 920, 1030, 2236
SspI	AATATT	1 cut(s)	2184
SspMI	CTAG	3 cut(s)	870, 878, 1323
StyD4I	CCNGG	5 cut(s)	193, 230, 248, 1040, 1247
TaaI	ACNGT	4 cut(s)	89, 598, 1729, 1784
TaiI	ACGT	4 cut(s)	48, 888, 2079, 2276
TaqI	TCGA	4 cut(s)	107, 263, 275, 1437
TaqII	GACCGA	2 cut(s)	52, 332
TatI	WGTACW	4 cut(s)	1389, 1801, 1885, 2010
TauI	GCSGC	3 cut(s)	190, 193, 205
TfiI	GAWTC	6 cut(s)	109, 874, 984, 997, 1004, 1937
Tru1I	TTAA	6 cut(s)	498, 593, 741, 1230, 1553, 2061
Tru9I	TTAA	6 cut(s)	498, 593, 741, 1230, 1553, 2061
TscAI	CASTG	2 cut(s)	2039, 2260
TseFI	GTSAC	1 cut(s)	685
TseI	GCWGC	3 cut(s)	386, 453, 1466
Tsp45I	GTSAC	1 cut(s)	685
TspDTI	ATGAA	9 cut(s)	542, 1004, 1202, 1408, 1436, 1838, 1946, 2102, 2219
TspGWI	ACGGA	1 cut(s)	2148
TspRI	CASTG	2 cut(s)	2039, 2260
Van91I	CCANNNNNTGG	1 cut(s)	2053
Vha464I	CTTAAG	2 cut(s)	497, 740
VpaK11BI	GGWCC	3 cut(s)	252, 1212, 1780
XapI	RAATTY	7 cut(s)	324, 940, 1111, 1200, 1866, 1879, 1946
XmiI	GTMKAC	2 cut(s)	1437, 1521
XmnI	GAANNNNTTC	1 cut(s)	1868
XspI	CTAG	3 cut(s)	870, 878, 1323
ZraI	GACGTC	1 cut(s)	2077
ZrmI	AGTACT	2 cut(s)	1887, 2012

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.