Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003680

Physical Location & Seq

Genomic Coordinates Forward (+)

6808138 .. 6811775

Gene Structure 3638 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1086900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGGAAGGTGGAAAAGGCGTGGGGGAGAAGAAAGGAACAAACGATGTGGTTTTGCAGATTTCGGGCGGAGAAGAACACACGAGATACAGTAACGAAATCAGAGACTCGAATCTCCAGCTGACAGAGCTTCAGAGCCTCAGGGTCTCCGGCTCTGCTTCTCCAGAAATCCCAAAGCCGAGTCCCTCGCCGCCGAAAATCCCAGCCGAAACCACTTCCCGGCGAGCTTCGTTTTCCCGGTCCGCGTTCTCGAAGCCCAAGTCCAGGTTCGTGGAGCCGGTTTCTCCTGCTGAAAAGAAGGCGACCGACGAAATTCCCCAGTTGAAGTCAACTGCTAATTCCCCCAATGTGTCATCCAGCGGGGAGGACGACGATGAGGAGGTTTACAAGATTGCAAGGCTTAAGGTGCGTGAGAAGTGTGGTAAGAAGATGAAAAAGTTGGCACTAATTGAGTTGATTGCATTTGTTTGTATTGTTGGGTTTTTGATTGCTTGCTTAACTGTTCCTAAGTTGGAAAAGAAGATGTATTGGGGGTTGGAATTGTGGAAATGGTCTGTCTTGGTGTTGGTTGTTTTGTGTGGTGGATTGGTCACAGAATGGTTTATCAATGTTCTGGTTTTCGTGATTGAGTTGAACTTTTTGCTTAAGAAGAATGTTCTGTATTTCGTTAACGGGTTGAAGAGAAGCGTACAGATTTTTATTTGGTTGGGTTTGGTTCTTCTGGCATGGGGTTTGTTGTTCCACCGCGGAGTGAAGAGGTCAAGGAAAACCTCTAGGATTCTAGGCTACGTTACAAGGGGTCTGGCTTCGTGTCTAATTGGAGCGGGCATATGGCTGGTGAAGAATTTGTTTGTCAAAGTGGTAGCTTCTTTGTTTCAATGCAATAGATTCTTTGATCGGATTCAAGAATCAATTTTTCATCAGTATGTTGTCCGCACTCTTTCCTGGATTTCTCTGATGGAAAGGTGTAAACAAGTCAGGAAGATCCCAAGTAGGGGCCAGTTGAGTTTCAAAAATTTGAAGGGAGGGAAAAAGGAGGGGAAGGGCGGGCTCAAAGAAGAGGTGATTGATGTAGAAAAGCTAAAAAAGATGAACCAAAAGAAAATTTCTGCTCGGACCTTGAAAGGGTTGATTAACGTGGTAAGGAGTTCCGGGCTATTTACCATCTCCGACACACTTGAGAGCGAGGAGAGTGAGCAGAAAGGTAAGGAGATTACCAGCGAGCAGGAAGCAAAGGATGTGGCTAAACATATTTTCCAGAATGTCGCCAACCATGGCGAGCACATTGAGAAAGAGATCCTCTTGAACCACATGAAAAAGGAGGAGGTCGACAATGTATTACATCTGTTTGAAGGGGCACCAGAAACTGGCAAGATCAAGAAAAAAGCTTTTAAGAAGTGGCTGGTGAAAGTCTACCTTGAACGCAAATCGCTCTCACGTTCGTTACATGACAGTAAAACAGCGATAGAGGAGCTGAACAGGCTTGCCTCAGGGGTTTTTCTTCTTGTGATCATCATCGTGTGGCTACTTATGATGGGATTTTTGACAACCAACATACTGGTCTTTATATCATCTCAGCTTTTACTGATGGCCTTTGTATTTGGTAATACTGCTAAAACTGTGTTTGAAGCAATCATTTTCGTCTTTGTGAGGCACCCTTTTGATGTAGGGGACCGTTGTGTCGTTGATGGAGTACAGCTCGTAGTCGAAGAGATGAACATAATGACAACGATCTTCCTAAGATATGACAACGAGATAATTTCCTATCCAAATTCGGTACTGGCTTCTAAACCCATCAGCAACTTCTCCAGGAGTCCGGATATGGGTGATTCTGTTGAATTTGCTGTTCACGTTTCTACAACGGCTGATACTATTGTGGCTCTAAAAGCCAGAATAAAAGAGTACTTGGACAGCAAGAGTCAGCACTGGCGTCCTGCCCATAGCGTGGTGGTTAAGGATATTGAGGACGTCAACAAAATGAAAATGGCTCTTTATGTTACGCATACCATGAACTTTCATAACTACCCAGAAAAGAGCAGTAGGAGATCGGATTTGGTCTTAGAGCTGAAGAAAATATTTGAAGATCTCGGTATAAAATATAAACTCCTGCCTCAAGAAGTTCATCTCCGCTATGTCGGATCATCCACTGCAGAACTTCCACCAATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel PsbX

Protein Analysis

722

Amino Acids

81.55

Weight (kDa)

9.48

Isoelectric Point (pI)

44.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	545 - 603	6.1e-10	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003680)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g08260 FvH4_1g08260 FvH4_1g08260 FvH4_1g08260
malus_domestica	MD02G1086900.v1.1 MD02G1087000.v1.1 MD15G1214500.v1.1 MD15G1214600.v1.1
prunus_persica	Prupe.7G202700_v2.0.a1
pyrus_communis	pycom02g06990 pycom02g07000 pycom15g19040
rosa_chinensis	RchiOBHm_Chr2g0094331
rosa_laevigata	RLG00000016480
rosa_multiflora	Rmu_sc0003819.1_g000016 Rmu_sc0025410.1_g000001 Rmu_sc0025411.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147230 Rroxscaffold_7G00185070
rosa_rugosa	Rorug02G0043300 Rorug02G0043300
rosa_samantha	Rh2AG091000 Rh2AG091100 Rh2BG090900 Rh2CG093400 Rh2CG093500 Rh2DG089900 Rh6AG505100 Rh6BG272500 Rh6CG273600 Rh6CG521500 Rh6CG521800 Rh6DG266600 Rh6DG507600
rosa_wichuraiana	Rw6G043950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1676
AatII	GACGTC	1 cut(s)	1968
AccB1I	GGYRCC	2 cut(s)	1354, 1650
AccB7I	CCANNNNNTGG	2 cut(s)	1364, 1942
AccBSI	CCGCTC	1 cut(s)	821
AccI	GTMKAC	2 cut(s)	1326, 1410
AccII	CGCG	2 cut(s)	242, 744
AccIII	TCCGGA	1 cut(s)	1813
AclWI	GGATC	3 cut(s)	976, 1288, 2143
AcsI	RAATTY	6 cut(s)	309, 841, 1012, 1101, 1768, 1835
AcuI	CTGAAG	2 cut(s)	113, 2084
AcyI	GRCGYC	2 cut(s)	1927, 1965
AfaI	GTAC	4 cut(s)	687, 1692, 1776, 1901
AfiI	CCNNNNNNNGG	8 cut(s)	216, 261, 991, 992, 1122, 1364, 1820, 1942
AflII	CTTAAG	2 cut(s)	398, 641
AjnI	CCWGG	3 cut(s)	260, 941, 1805
AjuI	GAANNNNNNNTTGG	2 cut(s)	248, 280
Alw21I	GWGCWC	1 cut(s)	1281
Alw26I	GTCTC	2 cut(s)	96, 148
AlwI	GGATC	3 cut(s)	976, 1288, 2143
AlwNI	CAGNNNCTG	1 cut(s)	1364
Aor13HI	TCCGGA	1 cut(s)	1813
AoxI	GGCC	2 cut(s)	994, 1587
ApoI	RAATTY	6 cut(s)	309, 841, 1012, 1101, 1768, 1835
ArsI	GACNNNNNNTTYG	2 cut(s)	242, 274
AspS9I	GGNCC	4 cut(s)	237, 994, 1113, 1669
AsuC2I	CCSGG	3 cut(s)	217, 235, 1150
AsuHPI	GGTGA	4 cut(s)	847, 1072, 1414, 1835
AvaII	GGWCC	3 cut(s)	237, 1113, 1669
AxyI	CCTNAGG	2 cut(s)	137, 1486
BaeGI	GKGCMC	1 cut(s)	1357
BaeI	ACNNNNGTAYC	2 cut(s)	1857, 1890
BanI	GGYRCC	2 cut(s)	1354, 1650
BanII	GRGCYC	1 cut(s)	1050
BauI	CACGAG	1 cut(s)	79
Bbv12I	GWGCWC	1 cut(s)	1281
BccI	CCATC	6 cut(s)	949, 1169, 1525, 1579, 1679, 1799
BceAI	ACGGC	1 cut(s)	1875
BciT130I	CCWGG	3 cut(s)	262, 943, 1807
BclI	TGATCA	1 cut(s)	1506
BcnI	CCSGG	3 cut(s)	217, 235, 1150
BcoDI	GTCTC	2 cut(s)	96, 148
BfaI	CTAG	2 cut(s)	771, 779
BfmI	CTRYAG	1 cut(s)	2145
BfrI	CTTAAG	2 cut(s)	398, 641
BglII	AGATCT	1 cut(s)	2080
BisI	GCNGC	1 cut(s)	188
BlsI	GCNGC	1 cut(s)	189
BmcAI	AGTACT	1 cut(s)	1901
Bme1390I	CCNGG	6 cut(s)	217, 235, 262, 943, 1150, 1807
Bme18I	GGWCC	3 cut(s)	237, 1113, 1669
BmgT120I	GGNCC	4 cut(s)	237, 994, 1113, 1669
BmiI	GGNNCC	5 cut(s)	273, 995, 1356, 1652, 1670
BmrFI	CCNGG	6 cut(s)	217, 235, 262, 943, 1150, 1807
BmrI	ACTGGG	1 cut(s)	310
BmuI	ACTGGG	1 cut(s)	310
BplI	GAGNNNNNCTC	2 cut(s)	1680, 1712
BpmI	CTGGAG	3 cut(s)	98, 144, 1789
BpuEI	CTTGAG	2 cut(s)	1196, 2094
BpuMI	CCSGG	3 cut(s)	217, 235, 1150
BsaBI	GATNNNNATC	1 cut(s)	1511
BsaHI	GRCGYC	2 cut(s)	1927, 1965
BsaI	GGTCTC	1 cut(s)	148
BsaJI	CCNNGG	2 cut(s)	742, 1270
BsaWI	WCCGGW	1 cut(s)	1813
Bsc4I	CCNNNNNNNGG	8 cut(s)	216, 261, 991, 992, 1122, 1364, 1820, 1942
Bse118I	RCCGGY	1 cut(s)	274
Bse1I	ACTGG	6 cut(s)	316, 997, 1369, 1560, 1782, 1928
Bse21I	CCTNAGG	2 cut(s)	137, 1486
Bse8I	GATNNNNATC	1 cut(s)	1511
BseAI	TCCGGA	1 cut(s)	1813
BseBI	CCWGG	3 cut(s)	262, 943, 1807
BseDI	CCNNGG	2 cut(s)	742, 1270
BseGI	GGATG	3 cut(s)	350, 1240, 2138
BseJI	GATNNNNATC	1 cut(s)	1511
BseLI	CCNNNNNNNGG	8 cut(s)	216, 261, 991, 992, 1122, 1364, 1820, 1942
BseMII	CTCAG	3 cut(s)	151, 1500, 1586
BseNI	ACTGG	6 cut(s)	316, 997, 1369, 1560, 1782, 1928
BseRI	GAGGAG	4 cut(s)	389, 1199, 1334, 1481
BseSI	GKGCMC	1 cut(s)	1357
BseYI	CCCAGC	1 cut(s)	199
Bsh1236I	CGCG	2 cut(s)	242, 744
Bsh1285I	CGRYCG	1 cut(s)	303
BshFI	GGCC	2 cut(s)	996, 1589
BshNI	GGYRCC	2 cut(s)	1354, 1650
BsiEI	CGRYCG	1 cut(s)	303
BsiHKAI	GWGCWC	1 cut(s)	1281
BsiSI	CCGG	6 cut(s)	147, 217, 235, 275, 1149, 1814
BslFI	GGGAC	2 cut(s)	165, 1682
BslI	CCNNNNNNNGG	8 cut(s)	216, 261, 991, 992, 1122, 1364, 1820, 1942
BsmAI	GTCTC	2 cut(s)	96, 148
BsmFI	GGGAC	2 cut(s)	165, 1682
BsnI	GGCC	2 cut(s)	996, 1589
Bso31I	GGTCTC	1 cut(s)	148
Bsp1286I	GDGCHC	3 cut(s)	1050, 1281, 1357
Bsp13I	TCCGGA	1 cut(s)	1813
Bsp143I	GATC	9 cut(s)	892, 981, 1293, 1371, 1506, 1728, 2042, 2080, 2135
Bsp19I	CCATGG	1 cut(s)	1270
BspANI	GGCC	2 cut(s)	996, 1589
BspCNI	CTCAG	3 cut(s)	150, 1499, 1585
BspEI	TCCGGA	1 cut(s)	1813
BspFNI	CGCG	2 cut(s)	242, 744
BspLI	GGNNCC	5 cut(s)	273, 995, 1356, 1652, 1670
BspMAI	CTGCAG	1 cut(s)	2149
BspPI	GGATC	3 cut(s)	976, 1288, 2143
BspT107I	GGYRCC	2 cut(s)	1354, 1650
BspTI	CTTAAG	2 cut(s)	398, 641
BspTNI	GGTCTC	1 cut(s)	148
BsrBI	CCGCTC	1 cut(s)	821
BsrFI	RCCGGY	1 cut(s)	274
BsrI	ACTGG	6 cut(s)	316, 997, 1369, 1560, 1782, 1928
BssAI	RCCGGY	1 cut(s)	274
BssECI	CCNNGG	2 cut(s)	742, 1270
BssMI	GATC	9 cut(s)	892, 981, 1293, 1371, 1506, 1728, 2042, 2080, 2135
BssNI	GRCGYC	2 cut(s)	1927, 1965
BssSI	CACGAG	1 cut(s)	79
BssT1I	CCWWGG	1 cut(s)	1270
Bst2BI	CACGAG	1 cut(s)	79
Bst2UI	CCWGG	3 cut(s)	262, 943, 1807
Bst4CI	ACNGT	5 cut(s)	89, 499, 1451, 1618, 1673
Bst6I	CTCTTC	4 cut(s)	671, 746, 1050, 1701
BstACI	GRCGYC	2 cut(s)	1927, 1965
BstAFI	CTTAAG	2 cut(s)	398, 641
BstC8I	GCNNGC	7 cut(s)	222, 490, 823, 1046, 1220, 1277, 1482
BstDEI	CTNAG	6 cut(s)	137, 504, 1486, 1572, 1736, 2056
BstDSI	CCRYGG	2 cut(s)	742, 1270
BstF5I	GGATG	3 cut(s)	350, 1240, 2138
BstFNI	CGCG	2 cut(s)	242, 744
BstKTI	GATC	9 cut(s)	895, 984, 1296, 1374, 1509, 1731, 2045, 2083, 2138
BstMAI	GTCTC	2 cut(s)	96, 148
BstMBI	GATC	9 cut(s)	892, 981, 1293, 1371, 1506, 1728, 2042, 2080, 2135
BstMCI	CGRYCG	1 cut(s)	303
BstMWI	GCNNNNNNNGC	4 cut(s)	124, 403, 1477, 1883
BstNI	CCWGG	3 cut(s)	262, 943, 1807
BstSCI	CCNGG	6 cut(s)	215, 233, 260, 941, 1148, 1805
BstSFI	CTRYAG	1 cut(s)	2145
BstSLI	GKGCMC	1 cut(s)	1357
BstUI	CGCG	2 cut(s)	242, 744
BstX2I	RGATCY	3 cut(s)	981, 1293, 2080
BstXI	CCANNNNNNTGG	2 cut(s)	268, 1555
BstYI	RGATCY	3 cut(s)	981, 1293, 2080
Bsu36I	CCTNAGG	2 cut(s)	137, 1486
BsuRI	GGCC	2 cut(s)	996, 1589
BtgI	CCRYGG	2 cut(s)	742, 1270
BtsCI	GGATG	3 cut(s)	350, 1240, 2138
BtsI	GCAGTG	1 cut(s)	2142
BtsIMutI	CAGTG	2 cut(s)	1921, 2142
Cac8I	GCNNGC	7 cut(s)	222, 490, 823, 1046, 1220, 1277, 1482
CaiI	CAGNNNCTG	1 cut(s)	1364
Cfr10I	RCCGGY	1 cut(s)	274
Cfr13I	GGNCC	4 cut(s)	237, 994, 1113, 1669
Cfr42I	CCGCGG	1 cut(s)	745
CpoI	CGGWCCG	1 cut(s)	237
CseI	GACGC	1 cut(s)	1916
Csp6I	GTAC	4 cut(s)	686, 1691, 1775, 1900
CspCI	CAANNNNNGTGG	4 cut(s)	27, 62, 1218, 1253
CspI	CGGWCCG	1 cut(s)	237
CviAII	CATG	5 cut(s)	723, 1271, 1309, 1445, 2005
CviQI	GTAC	4 cut(s)	686, 1691, 1775, 1900
DdeI	CTNAG	6 cut(s)	137, 504, 1486, 1572, 1736, 2056
DpnI	GATC	9 cut(s)	894, 983, 1295, 1373, 1508, 1730, 2044, 2082, 2137
DpnII	GATC	9 cut(s)	892, 981, 1293, 1371, 1506, 1728, 2042, 2080, 2135
DrdI	GACNNNNNNGTC	1 cut(s)	1676
DseDI	GACNNNNNNGTC	1 cut(s)	1676
Eam1104I	CTCTTC	4 cut(s)	671, 746, 1050, 1701
EarI	CTCTTC	4 cut(s)	671, 746, 1050, 1701
EciI	GGCGGA	1 cut(s)	81
Eco130I	CCWWGG	1 cut(s)	1270
Eco24I	GRGCYC	1 cut(s)	1050
Eco31I	GGTCTC	1 cut(s)	148
Eco47I	GGWCC	3 cut(s)	237, 1113, 1669
Eco57I	CTGAAG	2 cut(s)	113, 2084
Eco81I	CCTNAGG	2 cut(s)	137, 1486
EcoRII	CCWGG	3 cut(s)	260, 941, 1805
EcoT14I	CCWWGG	1 cut(s)	1270
EcoT38I	GRGCYC	1 cut(s)	1050
ErhI	CCWWGG	1 cut(s)	1270
FaeI	CATG	5 cut(s)	726, 1274, 1312, 1448, 2008
FalI	AAGNNNNNCTT	2 cut(s)	1398, 1430
FaqI	GGGAC	2 cut(s)	165, 1682
FatI	CATG	5 cut(s)	722, 1270, 1308, 1444, 2004
FauI	CCCGC	3 cut(s)	350, 814, 1037
FauNDI	CATATG	1 cut(s)	827
FbaI	TGATCA	1 cut(s)	1506
FblI	GTMKAC	2 cut(s)	1326, 1410
Fnu4HI	GCNGC	1 cut(s)	188
FokI	GGATG	3 cut(s)	337, 1247, 2125
FriOI	GRGCYC	1 cut(s)	1050
Fsp4HI	GCNGC	1 cut(s)	188
FspBI	CTAG	2 cut(s)	771, 779
GluI	GCNGC	1 cut(s)	188
GsaI	CCCAGC	1 cut(s)	203
GsuI	CTGGAG	3 cut(s)	98, 144, 1789
HaeIII	GGCC	2 cut(s)	996, 1589
HapII	CCGG	6 cut(s)	147, 217, 235, 275, 1149, 1814
HgaI	GACGC	1 cut(s)	1916
Hin1I	GRCGYC	2 cut(s)	1927, 1965
Hin1II	CATG	5 cut(s)	726, 1274, 1312, 1448, 2008
HincII	GTYRAC	4 cut(s)	327, 667, 1327, 1969
HindII	GTYRAC	4 cut(s)	327, 667, 1327, 1969
HindIII	AAGCTT	1 cut(s)	1383
HpaI	GTTAAC	1 cut(s)	667
HpaII	CCGG	6 cut(s)	147, 217, 235, 275, 1149, 1814
HphI	GGTGA	4 cut(s)	847, 1072, 1414, 1835
Hpy166II	GTNNAC	8 cut(s)	327, 382, 667, 968, 1327, 1411, 1846, 1969
Hpy188I	TCNGA	8 cut(s)	101, 132, 897, 954, 1113, 1168, 2047, 2135
Hpy8I	GTNNAC	8 cut(s)	327, 382, 667, 968, 1327, 1411, 1846, 1969
Hpy99I	CGWCG	2 cut(s)	308, 371
HpyAV	CCTTC	4 cut(s)	289, 1012, 1033, 1343
HpyCH4III	ACNGT	5 cut(s)	89, 499, 1451, 1618, 1673
HpyCH4IV	ACGT	5 cut(s)	786, 1134, 1435, 1848, 1965
HpyCH4V	TGCA	5 cut(s)	55, 392, 458, 879, 2147
HpyF10VI	GCNNNNNNNGC	4 cut(s)	124, 403, 1477, 1883
HpyF3I	CTNAG	6 cut(s)	137, 504, 1486, 1572, 1736, 2056
HpySE526I	ACGT	5 cut(s)	786, 1134, 1435, 1848, 1965
Hsp92I	GRCGYC	2 cut(s)	1927, 1965
Hsp92II	CATG	5 cut(s)	726, 1274, 1312, 1448, 2008
Kpn2I	TCCGGA	1 cut(s)	1813
Ksp22I	TGATCA	1 cut(s)	1506
KspAI	GTTAAC	1 cut(s)	667
KspI	CCGCGG	1 cut(s)	745
Kzo9I	GATC	9 cut(s)	892, 981, 1293, 1371, 1506, 1728, 2042, 2080, 2135
LmnI	GCTCC	3 cut(s)	271, 818, 1468
MaeI	CTAG	2 cut(s)	771, 779
MaeII	ACGT	5 cut(s)	786, 1134, 1435, 1848, 1965
MaeIII	GTNAC	5 cut(s)	89, 586, 787, 1440, 1993
MalI	GATC	9 cut(s)	894, 983, 1295, 1373, 1508, 1730, 2044, 2082, 2137
MbiI	CCGCTC	1 cut(s)	821
MboI	GATC	9 cut(s)	892, 981, 1293, 1371, 1506, 1728, 2042, 2080, 2135
MflI	RGATCY	3 cut(s)	981, 1293, 2080
MhlI	GDGCHC	3 cut(s)	1050, 1281, 1357
MlyI	GAGTC	4 cut(s)	98, 187, 1819, 1924
MmeI	TCCRAC	4 cut(s)	489, 513, 1191, 2113
MroI	TCCGGA	1 cut(s)	1813
MseI	TTAA	7 cut(s)	399, 494, 642, 666, 1131, 1389, 1950
MslI	CAYNNNNRTG	2 cut(s)	921, 1514
MspA1I	CMGCKG	3 cut(s)	118, 357, 744
MspCI	CTTAAG	2 cut(s)	398, 641
MspI	CCGG	6 cut(s)	147, 217, 235, 275, 1149, 1814
MspR9I	CCNGG	6 cut(s)	217, 235, 262, 943, 1150, 1807
MvaI	CCWGG	3 cut(s)	262, 943, 1807
MvnI	CGCG	2 cut(s)	242, 744
MwoI	GCNNNNNNNGC	4 cut(s)	124, 403, 1477, 1883
NciI	CCSGG	3 cut(s)	217, 235, 1150
NcoI	CCATGG	1 cut(s)	1270
NdeI	CATATG	1 cut(s)	827
NdeII	GATC	9 cut(s)	892, 981, 1293, 1371, 1506, 1728, 2042, 2080, 2135
NlaIII	CATG	5 cut(s)	726, 1274, 1312, 1448, 2008
NlaIV	GGNNCC	5 cut(s)	273, 995, 1356, 1652, 1670
NmeAIII	GCCGAG	1 cut(s)	201
NmuCI	GTSAC	1 cut(s)	586
PfeI	GAWTC	6 cut(s)	109, 775, 885, 898, 905, 1826
PflMI	CCANNNNNTGG	2 cut(s)	1364, 1942
PfoI	TCCNGGA	2 cut(s)	941, 1805
PkrI	GCNGC	1 cut(s)	189
PleI	GAGTC	4 cut(s)	98, 186, 1818, 1923
PpsI	GAGTC	4 cut(s)	98, 186, 1818, 1923
Psp6I	CCWGG	3 cut(s)	260, 941, 1805
PspFI	CCCAGC	1 cut(s)	199
PspGI	CCWGG	3 cut(s)	260, 941, 1805
PspN4I	GGNNCC	5 cut(s)	273, 995, 1356, 1652, 1670
PspPI	GGNCC	4 cut(s)	237, 994, 1113, 1669
PstI	CTGCAG	1 cut(s)	2149
PstNI	CAGNNNCTG	1 cut(s)	1364
PsuI	RGATCY	3 cut(s)	981, 1293, 2080
PvuII	CAGCTG	1 cut(s)	118
RsaI	GTAC	4 cut(s)	687, 1692, 1776, 1901
RsaNI	GTAC	4 cut(s)	686, 1691, 1775, 1900
RseI	CAYNNNNRTG	2 cut(s)	921, 1514
Rsr2I	CGGWCCG	1 cut(s)	237
RsrII	CGGWCCG	1 cut(s)	237
SacII	CCGCGG	1 cut(s)	745
SalI	GTCGAC	1 cut(s)	1325
SaqAI	TTAA	7 cut(s)	399, 494, 642, 666, 1131, 1389, 1950
SatI	GCNGC	1 cut(s)	188
Sau3AI	GATC	9 cut(s)	892, 981, 1293, 1371, 1506, 1728, 2042, 2080, 2135
Sau96I	GGNCC	4 cut(s)	237, 994, 1113, 1669
ScaI	AGTACT	1 cut(s)	1901
SchI	GAGTC	4 cut(s)	98, 187, 1819, 1924
ScrFI	CCNGG	6 cut(s)	217, 235, 262, 943, 1150, 1807
SduI	GDGCHC	3 cut(s)	1050, 1281, 1357
SfcI	CTRYAG	1 cut(s)	2145
Sfr303I	CCGCGG	1 cut(s)	745
SgrBI	CCGCGG	1 cut(s)	745
SinI	GGWCC	3 cut(s)	237, 1113, 1669
SmiMI	CAYNNNNRTG	2 cut(s)	921, 1514
SmlI	CTYRAG	4 cut(s)	398, 641, 1175, 2109
SmoI	CTYRAG	4 cut(s)	398, 641, 1175, 2109
SspI	AATATT	1 cut(s)	2073
SspMI	CTAG	2 cut(s)	771, 779
StyD4I	CCNGG	6 cut(s)	215, 233, 260, 941, 1148, 1805
StyI	CCWWGG	1 cut(s)	1270
TaaI	ACNGT	5 cut(s)	89, 499, 1451, 1618, 1673
TaiI	ACGT	5 cut(s)	789, 1137, 1438, 1851, 1968
TaqI	TCGA	4 cut(s)	107, 248, 1326, 1704
TaqII	GACCGA	1 cut(s)	317
TatI	WGTACW	2 cut(s)	1690, 1899
TauI	GCSGC	1 cut(s)	190
TfiI	GAWTC	6 cut(s)	109, 775, 885, 898, 905, 1826
Tru1I	TTAA	7 cut(s)	399, 494, 642, 666, 1131, 1389, 1950
Tru9I	TTAA	7 cut(s)	399, 494, 642, 666, 1131, 1389, 1950
TscAI	CASTG	2 cut(s)	1928, 2149
TseFI	GTSAC	1 cut(s)	586
Tsp45I	GTSAC	1 cut(s)	586
TspDTI	ATGAA	9 cut(s)	443, 905, 1103, 1325, 1727, 1991, 2003, 2021, 2108
TspRI	CASTG	2 cut(s)	1928, 2149
Van91I	CCANNNNNTGG	2 cut(s)	1364, 1942
Vha464I	CTTAAG	2 cut(s)	398, 641
VpaK11BI	GGWCC	3 cut(s)	237, 1113, 1669
XapI	RAATTY	6 cut(s)	309, 841, 1012, 1101, 1768, 1835
XmiI	GTMKAC	2 cut(s)	1326, 1410
XspI	CTAG	2 cut(s)	771, 779
ZraI	GACGTC	1 cut(s)	1966
ZrmI	AGTACT	1 cut(s)	1901

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.