Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003680

Physical Location & Seq

Genomic Coordinates Forward (+)

17239485 .. 17242290

Gene Structure 2806 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1214500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2160 bp

ATGGAAGGTGGAAAAGGCGTGGGGGAGAAGAAAGGAACAAACGATGTAGTTTTGCAGATTTCGGTCGGCGAAGAGCGCACAAGTTACAACAAAGAAACCAGAGACTTGAATCTTCAGCTGACGGAGCTTCAGTGCCTCAGAGTCTCCGGCTCTTCTTCGCGGTCGGTGTTCTCGAAACCCAAGTCGAGGTTCGTTGAGCCGTTTCCTCAGTTGAAGTCAACTGCTAATTCTTCCAATGTGGCATCGCCAAGCTCCAAAATCGCAGGCACAACACCGAGAGACGCTCTGAGGTCCGCCCCGATAACCCCAAGAGCACCGTTGGTCGACGGCGGCGAGGAGGACGATGATGAGGAGGTTTATAAGACTGCAAAGCTAAAGGTGTGTGCGAAGTCCGGTAAGAAGATAAAAAAGTTGGCATTGATTGAGTTGGTTGCATTTGTTTGCATTGTTGTGTTTTTTATTGCTGGCTTAACTGTACCTACGTTGGAAAATAAGATGTATTTGGGGTTGGAATTGTGGAAATGGTGTGTCTTGGTGTTGGTTGTTTTGTGTGGTAGATTGGTCACAGAATGGTTTATCAATGTTCTGGTTTTCGTGATTGAGTTGAACTTTTTGCTTAAGAAGAATGTTTTGTATTTTGTTTACGGGTTGAAGAGAAGTGTACAGATTTTTATTTGGTTGGGTTTGGTTCTTCTGGCATGGGGTTTGTTGTTCGACAACGGAGTGAAGAGGTCAAGGAAAACCTCTAGGATCCTAGGTTATGTTACACGGGGTCTGGCTTCGTGCCTAATTGGAGCGGCCATATGGCTGGTGAAGAATTTGTTTGTCAAAGTAGTAGCTTCTTCATTCCAATGCAATAGATTCTTTGATCGGATTCAAGAATCAATCTTTCATCAGTATGTTCTCCGCACCCTTTCCGGGCCTCCACTGATGGAAATGGCAGAAAGTGTCGGGAGGGCCCCAAGCAGCGGTCGGTTGAGTTTCAAAAATTTGAAGGGAGGGAAAAAGGAGGGGAATGAAGGGGCCAAAGAGGAGGTGATTGATGTAGAAAAGCTCAAAAAGATGAACCAAGAGAAAATTTCTGCTTGGACCTTGAAAGGGTTGATTAATGTAGTAAGGAGTTCGGGGTTATCTACCATCTCCAACACACTTGAGAGTCTTGACGATGAGGAGAGTGAGCAGAAAGGTAAGGAGATTACTAGTGAGTGGGAAGCAAAGGCTGCGGCTTATGATATTTTCCTGAATGTCACCAAGCGTAGCAACAAGTACATTGAGGAAGATGACCTCTTGCGCTTCATGAAAAAGGAGGAGGTCGACATTGTTTTACCTCTGTTTGAAGGGGCAGCGGAAACTGGCAAGATCAAGAGAAAAGCTTTGAAGAACTGGCTGGTGAATGTTTACCTTGAGCGCAAATCACTGGCGCATTCCTTAAATGACACTAAAACAGCAATTGAGGAGCTGAATAGGCTTGCCTCAGGGTTTTTGCTTCTGGTGATCATTATCGTGTGGCTACTTCTGATGGGATTGTTTACGACAAACATGCTAGTCCTTATAGCATCTCAGCTTTTAATGCTGGCGTTTGTATTTGGTAATACTGCAAAAACTGTGTTTGAAGCGATCATATTCGTCTTTGTGATGCACCCTTTTGATGTAGGGGACCGTTGTGTCATTGAAGGAGTACAGCTTATTGTTGAAGAGATGAACATATTGACAACAGTCTTCTTAAGATCTGACAACGAGAAAGTTTACTATCCAAATTCAGTACTGGCTAGTAAACCCATCAGCAACTTCTATAGGAGCCCGGAGATGGGTAATTCTGTTGATTTTGCTATTGACGTTTCTACAAAGATTGAGACCATTGTGGCTCTAAAAGCCAGAATAAAAGAGTACTTGGACAGCAAGACTCAGCACTGGCGTCCTGCGCATAGCGTGGTGGTTAAGGATATTGAGGACGTTAACAAAATGATAATGGCTCTTTATGTTACACATACCATAAACTTTCAGAACTATGGCGACAAGACCAGTCGGAGATCAGATTTGATCTTTGAGCTGAAGAAAATATTTGAAGATCTTGGTATAAAATATCACCTCCCGCATCAAGAAGTTCATCTCCGCTATGTCGGAGCATCCGCTGCAGAACTTCCACCCACATGGCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel PsbX

Protein Analysis

720

Amino Acids

80.97

Weight (kDa)

9.04

Isoelectric Point (pI)

39.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Msy1	PF25886	140 - 283	1.5e-06	Mechanosensitive ion channel protein Msy1-like, transmembrane domain
MS_channel_2nd	PF00924	541 - 597	1.1e-09	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003680)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g08260 FvH4_1g08260 FvH4_1g08260 FvH4_1g08260
malus_domestica	MD02G1086900.v1.1 MD02G1087000.v1.1 MD15G1214500.v1.1 MD15G1214600.v1.1
prunus_persica	Prupe.7G202700_v2.0.a1
pyrus_communis	pycom02g06990 pycom02g07000 pycom15g19040
rosa_chinensis	RchiOBHm_Chr2g0094331
rosa_laevigata	RLG00000016480
rosa_multiflora	Rmu_sc0003819.1_g000016 Rmu_sc0025410.1_g000001 Rmu_sc0025411.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147230 Rroxscaffold_7G00185070
rosa_rugosa	Rorug02G0043300 Rorug02G0043300
rosa_samantha	Rh2AG091000 Rh2AG091100 Rh2BG090900 Rh2CG093400 Rh2CG093500 Rh2DG089900 Rh6AG505100 Rh6BG272500 Rh6CG273600 Rh6CG521500 Rh6CG521800 Rh6DG266600 Rh6DG507600
rosa_wichuraiana	Rw6G043950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	360
AasI	GACNNNNNNGTC	1 cut(s)	1664
Acc16I	TGCGCA	1 cut(s)	1923
AccBSI	CCGCTC	1 cut(s)	797
AccI	GTMKAC	2 cut(s)	324, 1314
AccII	CGCG	1 cut(s)	160
AclWI	GGATC	2 cut(s)	745, 758
AcoI	YGGCCR	1 cut(s)	798
AcsI	RAATTY	4 cut(s)	817, 988, 1077, 1756
AcuI	CTGAAG	3 cut(s)	98, 113, 2072
AcyI	GRCGYC	1 cut(s)	1915
AfaI	GTAC	6 cut(s)	477, 663, 1268, 1680, 1764, 1889
AfiI	CCNNNNNNNGG	7 cut(s)	186, 918, 968, 1098, 1807, 1808, 2154
AflII	CTTAAG	2 cut(s)	617, 1723
AhlI	ACTAGT	1 cut(s)	1199
AjuI	GAANNNNNNNTTGG	2 cut(s)	173, 205
Alw21I	GWGCWC	1 cut(s)	316
Alw26I	GTCTC	4 cut(s)	96, 148, 273, 1847
AlwI	GGATC	2 cut(s)	745, 758
AoxI	GGCC	4 cut(s)	798, 920, 957, 1023
ApaI	GGGCCC	1 cut(s)	961
ApeKI	GCWGC	4 cut(s)	966, 1220, 1343, 2132
ApoI	RAATTY	4 cut(s)	817, 988, 1077, 1756
ArsI	GACNNNNNNTTYG	2 cut(s)	167, 199
AseI	ATTAAT	1 cut(s)	1107
AspA2I	CCTAGG	1 cut(s)	754
AspLEI	GCGC	5 cut(s)	78, 1293, 1410, 1423, 1924
AspS9I	GGNCC	7 cut(s)	291, 920, 957, 958, 1023, 1089, 1657
AsuC2I	CCSGG	2 cut(s)	919, 1802
AsuHPI	GGTGA	6 cut(s)	823, 1048, 1240, 1402, 1504, 2078
AvaII	GGWCC	3 cut(s)	291, 1089, 1657
AvrII	CCTAGG	1 cut(s)	754
AxyI	CCTNAGG	1 cut(s)	1474
BaeGI	GKGCMC	1 cut(s)	961
BamHI	GGATCC	1 cut(s)	750
BanII	GRGCYC	2 cut(s)	961, 1802
BbsI	GAAGAC	1 cut(s)	1711
Bbv12I	GWGCWC	1 cut(s)	316
BbvI	GCAGC	4 cut(s)	978, 1207, 1355, 2119
BccI	CCATC	5 cut(s)	925, 1145, 1513, 1787, 1801
BceAI	ACGGC	2 cut(s)	184, 343
BcgI	CGANNNNNNTGC	2 cut(s)	1522, 1556
BclI	TGATCA	1 cut(s)	1494
BcnI	CCSGG	2 cut(s)	919, 1802
BcoDI	GTCTC	4 cut(s)	96, 148, 273, 1847
BcuI	ACTAGT	1 cut(s)	1199
BfaI	CTAG	5 cut(s)	747, 755, 1200, 1544, 1770
BfmI	CTRYAG	2 cut(s)	1792, 2133
BfrI	CTTAAG	2 cut(s)	617, 1723
BglII	AGATCT	2 cut(s)	1727, 2068
BisI	GCNGC	7 cut(s)	331, 798, 967, 1221, 1224, 1344, 2133
BlnI	CCTAGG	1 cut(s)	754
BlsI	GCNGC	7 cut(s)	332, 799, 968, 1222, 1225, 1345, 2134
BmcAI	AGTACT	2 cut(s)	1764, 1889
Bme1390I	CCNGG	2 cut(s)	919, 1802
Bme18I	GGWCC	3 cut(s)	291, 1089, 1657
BmgT120I	GGNCC	7 cut(s)	291, 920, 957, 958, 1023, 1089, 1657
BmiI	GGNNCC	6 cut(s)	752, 959, 960, 1024, 1658, 1799
BmrFI	CCNGG	2 cut(s)	919, 1802
BmsI	GCATC	5 cut(s)	251, 1565, 1626, 2104, 2135
BoxI	GACNNNNGTC	1 cut(s)	1715
BpiI	GAAGAC	1 cut(s)	1711
BplI	GAGNNNNNCTC	2 cut(s)	268, 300
BpuEI	CTTGAG	2 cut(s)	1172, 1424
BpuMI	CCSGG	2 cut(s)	919, 1802
BsaBI	GATNNNNATC	1 cut(s)	1499
BsaHI	GRCGYC	1 cut(s)	1915
BsaI	GGTCTC	1 cut(s)	1847
BsaJI	CCNNGG	1 cut(s)	754
BsaWI	WCCGGW	1 cut(s)	392
BsaXI	ACNNNNNCTCC	2 cut(s)	116, 146
Bsc4I	CCNNNNNNNGG	7 cut(s)	186, 918, 968, 1098, 1807, 1808, 2154
Bse1I	ACTGG	6 cut(s)	1357, 1388, 1422, 1770, 1916, 2022
Bse21I	CCTNAGG	1 cut(s)	1474
Bse8I	GATNNNNATC	1 cut(s)	1499
BseDI	CCNNGG	1 cut(s)	754
BseGI	GGATG	1 cut(s)	2126
BseJI	GATNNNNATC	1 cut(s)	1499
BseLI	CCNNNNNNNGG	7 cut(s)	186, 918, 968, 1098, 1807, 1808, 2154
BseMII	CTCAG	6 cut(s)	151, 221, 278, 1488, 1574, 1919
BseNI	ACTGG	6 cut(s)	1357, 1388, 1422, 1770, 1916, 2022
BseRI	GAGGAG	6 cut(s)	350, 365, 1046, 1184, 1322, 1469
BseSI	GKGCMC	1 cut(s)	961
BseXI	GCAGC	4 cut(s)	978, 1207, 1355, 2119
Bsh1236I	CGCG	1 cut(s)	160
Bsh1285I	CGRYCG	3 cut(s)	66, 164, 973
BshFI	GGCC	4 cut(s)	800, 922, 959, 1025
BsiEI	CGRYCG	3 cut(s)	66, 164, 973
BsiHKAI	GWGCWC	1 cut(s)	316
BsiSI	CCGG	4 cut(s)	147, 393, 918, 1802
BslFI	GGGAC	1 cut(s)	1670
BslI	CCNNNNNNNGG	7 cut(s)	186, 918, 968, 1098, 1807, 1808, 2154
BsmAI	GTCTC	4 cut(s)	96, 148, 273, 1847
BsmBI	CGTCTC	1 cut(s)	273
BsmFI	GGGAC	1 cut(s)	1670
BsmI	GAATGC	1 cut(s)	1423
BsnI	GGCC	4 cut(s)	800, 922, 959, 1025
Bso31I	GGTCTC	1 cut(s)	1847
Bsp120I	GGGCCC	1 cut(s)	957
Bsp1286I	GDGCHC	3 cut(s)	316, 961, 1802
Bsp1407I	TGTACA	1 cut(s)	661
Bsp143I	GATC	9 cut(s)	750, 868, 1359, 1494, 1617, 1727, 2030, 2040, 2068
BspANI	GGCC	4 cut(s)	800, 922, 959, 1025
BspCNI	CTCAG	6 cut(s)	150, 220, 279, 1487, 1573, 1918
BspFNI	CGCG	1 cut(s)	160
BspHI	TCATGA	1 cut(s)	1296
BspLI	GGNNCC	6 cut(s)	752, 959, 960, 1024, 1658, 1799
BspMAI	CTGCAG	1 cut(s)	2137
BspPI	GGATC	2 cut(s)	745, 758
BspQI	GCTCTTC	2 cut(s)	66, 157
BspTI	CTTAAG	2 cut(s)	617, 1723
BspTNI	GGTCTC	1 cut(s)	1847
BsrBI	CCGCTC	1 cut(s)	797
BsrGI	TGTACA	1 cut(s)	661
BsrI	ACTGG	6 cut(s)	1357, 1388, 1422, 1770, 1916, 2022
BssECI	CCNNGG	1 cut(s)	754
BssMI	GATC	9 cut(s)	750, 868, 1359, 1494, 1617, 1727, 2030, 2040, 2068
BssNI	GRCGYC	1 cut(s)	1915
BssT1I	CCWWGG	1 cut(s)	754
Bst4CI	ACNGT	5 cut(s)	318, 475, 1606, 1661, 1717
Bst6I	CTCTTC	5 cut(s)	66, 157, 647, 722, 1689
BstACI	GRCGYC	1 cut(s)	1915
BstAFI	CTTAAG	2 cut(s)	617, 1723
BstAPI	GCANNNNNTGC	2 cut(s)	1220, 2132
BstAUI	TGTACA	1 cut(s)	661
BstC8I	GCNNGC	4 cut(s)	265, 466, 1470, 1575
BstDEI	CTNAG	6 cut(s)	137, 207, 287, 1474, 1560, 1905
BstF5I	GGATG	1 cut(s)	2126
BstFNI	CGCG	1 cut(s)	160
BstHHI	GCGC	5 cut(s)	78, 1293, 1410, 1423, 1924
BstKTI	GATC	9 cut(s)	753, 871, 1362, 1497, 1620, 1730, 2033, 2043, 2071
BstMAI	GTCTC	4 cut(s)	96, 148, 273, 1847
BstMBI	GATC	9 cut(s)	750, 868, 1359, 1494, 1617, 1727, 2030, 2040, 2068
BstMCI	CGRYCG	3 cut(s)	66, 164, 973
BstMWI	GCNNNNNNNGC	8 cut(s)	75, 124, 1220, 1465, 1570, 1871, 1921, 2132
BstNSI	RCATGY	1 cut(s)	1543
BstPAI	GACNNNNGTC	1 cut(s)	1715
BstSCI	CCNGG	2 cut(s)	917, 1800
BstSFI	CTRYAG	2 cut(s)	1792, 2133
BstSLI	GKGCMC	1 cut(s)	961
BstUI	CGCG	1 cut(s)	160
BstV1I	GCAGC	4 cut(s)	978, 1207, 1355, 2119
BstV2I	GAAGAC	1 cut(s)	1711
BstX2I	RGATCY	3 cut(s)	750, 1727, 2068
BstXI	CCANNNNNNTGG	2 cut(s)	808, 2151
BstYI	RGATCY	3 cut(s)	750, 1727, 2068
Bsu36I	CCTNAGG	1 cut(s)	1474
BsuRI	GGCC	4 cut(s)	800, 922, 959, 1025
BtgZI	GCGATG	1 cut(s)	228
BtsCI	GGATG	1 cut(s)	2126
BtsIMutI	CAGTG	4 cut(s)	137, 926, 1415, 1909
Cac8I	GCNNGC	4 cut(s)	265, 466, 1470, 1575
CciI	TCATGA	1 cut(s)	1296
CfoI	GCGC	5 cut(s)	78, 1293, 1410, 1423, 1924
Cfr13I	GGNCC	7 cut(s)	291, 920, 957, 958, 1023, 1089, 1657
CseI	GACGC	2 cut(s)	290, 1904
Csp6I	GTAC	6 cut(s)	476, 662, 1267, 1679, 1763, 1888
CviAII	CATG	4 cut(s)	699, 1297, 1540, 2151
CviQI	GTAC	6 cut(s)	476, 662, 1267, 1679, 1763, 1888
DdeI	CTNAG	6 cut(s)	137, 207, 287, 1474, 1560, 1905
DpnI	GATC	9 cut(s)	752, 870, 1361, 1496, 1619, 1729, 2032, 2042, 2070
DpnII	GATC	9 cut(s)	750, 868, 1359, 1494, 1617, 1727, 2030, 2040, 2068
DrdI	GACNNNNNNGTC	1 cut(s)	1664
DseDI	GACNNNNNNGTC	1 cut(s)	1664
EaeI	YGGCCR	1 cut(s)	798
Eam1104I	CTCTTC	5 cut(s)	66, 157, 647, 722, 1689
EarI	CTCTTC	5 cut(s)	66, 157, 647, 722, 1689
EciI	GGCGGA	1 cut(s)	283
Eco130I	CCWWGG	1 cut(s)	754
Eco24I	GRGCYC	2 cut(s)	961, 1802
Eco31I	GGTCTC	1 cut(s)	1847
Eco47I	GGWCC	3 cut(s)	291, 1089, 1657
Eco57I	CTGAAG	3 cut(s)	98, 113, 2072
Eco81I	CCTNAGG	1 cut(s)	1474
EcoO109I	RGGNCCY	2 cut(s)	957, 958
EcoT14I	CCWWGG	1 cut(s)	754
EcoT38I	GRGCYC	2 cut(s)	961, 1802
ErhI	CCWWGG	1 cut(s)	754
Esp3I	CGTCTC	1 cut(s)	273
FaeI	CATG	4 cut(s)	702, 1300, 1543, 2154
FaqI	GGGAC	1 cut(s)	1670
FatI	CATG	4 cut(s)	698, 1296, 1539, 2150
FauI	CCCGC	1 cut(s)	2100
FauNDI	CATATG	1 cut(s)	803
FbaI	TGATCA	1 cut(s)	1494
FblI	GTMKAC	2 cut(s)	324, 1314
Fnu4HI	GCNGC	7 cut(s)	331, 798, 967, 1221, 1224, 1344, 2133
FokI	GGATG	1 cut(s)	2113
FriOI	GRGCYC	2 cut(s)	961, 1802
Fsp4HI	GCNGC	7 cut(s)	331, 798, 967, 1221, 1224, 1344, 2133
FspBI	CTAG	5 cut(s)	747, 755, 1200, 1544, 1770
FspI	TGCGCA	1 cut(s)	1923
GlaI	GCGC	5 cut(s)	77, 1292, 1409, 1422, 1923
GluI	GCNGC	7 cut(s)	331, 798, 967, 1221, 1224, 1344, 2133
HaeIII	GGCC	4 cut(s)	800, 922, 959, 1025
HapII	CCGG	4 cut(s)	147, 393, 918, 1802
HgaI	GACGC	2 cut(s)	290, 1904
HhaI	GCGC	5 cut(s)	78, 1293, 1410, 1423, 1924
Hin1I	GRCGYC	1 cut(s)	1915
Hin1II	CATG	4 cut(s)	702, 1300, 1543, 2154
Hin6I	GCGC	5 cut(s)	76, 1291, 1408, 1421, 1922
HinP1I	GCGC	5 cut(s)	76, 1291, 1408, 1421, 1922
HincII	GTYRAC	4 cut(s)	219, 325, 1315, 1957
HindII	GTYRAC	4 cut(s)	219, 325, 1315, 1957
HindIII	AAGCTT	1 cut(s)	1371
HinfI	GANTC	7 cut(s)	109, 141, 861, 874, 881, 1156, 1903
HpaI	GTTAAC	1 cut(s)	1957
HpaII	CCGG	4 cut(s)	147, 393, 918, 1802
HphI	GGTGA	6 cut(s)	823, 1048, 1240, 1402, 1504, 2078
Hpy188I	TCNGA	9 cut(s)	140, 288, 873, 1518, 1732, 2004, 2028, 2035, 2123
Hpy188III	TCNNGA	9 cut(s)	172, 595, 878, 952, 1160, 1240, 1297, 1363, 2099
Hpy99I	CGWCG	1 cut(s)	329
HpyAV	CCTTC	4 cut(s)	988, 1013, 1331, 1667
HpyCH4III	ACNGT	5 cut(s)	318, 475, 1606, 1661, 1717
HpyCH4IV	ACGT	3 cut(s)	482, 1836, 1953
HpyCH4V	TGCA	8 cut(s)	55, 368, 434, 444, 855, 1598, 1639, 2135
HpyF10VI	GCNNNNNNNGC	8 cut(s)	75, 124, 1220, 1465, 1570, 1871, 1921, 2132
HpyF3I	CTNAG	6 cut(s)	137, 207, 287, 1474, 1560, 1905
HpySE526I	ACGT	3 cut(s)	482, 1836, 1953
Hsp92I	GRCGYC	1 cut(s)	1915
Hsp92II	CATG	4 cut(s)	702, 1300, 1543, 2154
HspAI	GCGC	5 cut(s)	76, 1291, 1408, 1421, 1922
Ksp22I	TGATCA	1 cut(s)	1494
KspAI	GTTAAC	1 cut(s)	1957
Kzo9I	GATC	9 cut(s)	750, 868, 1359, 1494, 1617, 1727, 2030, 2040, 2068
LguI	GCTCTTC	2 cut(s)	66, 157
LmnI	GCTCC	6 cut(s)	124, 257, 794, 1456, 1797, 2123
Lsp1109I	GCAGC	4 cut(s)	978, 1207, 1355, 2119
LweI	GCATC	5 cut(s)	251, 1565, 1626, 2104, 2135
MaeI	CTAG	5 cut(s)	747, 755, 1200, 1544, 1770
MaeII	ACGT	3 cut(s)	482, 1836, 1953
MaeIII	GTNAC	5 cut(s)	83, 562, 763, 1246, 1981
MalI	GATC	9 cut(s)	752, 870, 1361, 1496, 1619, 1729, 2032, 2042, 2070
MbiI	CCGCTC	1 cut(s)	797
MboI	GATC	9 cut(s)	750, 868, 1359, 1494, 1617, 1727, 2030, 2040, 2068
MfeI	CAATTG	1 cut(s)	1449
MflI	RGATCY	3 cut(s)	750, 1727, 2068
MhlI	GDGCHC	3 cut(s)	316, 961, 1802
MluCI	AATT	9 cut(s)	226, 512, 789, 817, 988, 1077, 1449, 1756, 1813
MlyI	GAGTC	3 cut(s)	150, 1165, 1897
MmeI	TCCRAC	5 cut(s)	465, 489, 1167, 2006, 2101
MseI	TTAA	8 cut(s)	470, 618, 1107, 1430, 1568, 1724, 1938, 1956
MslI	CAYNNNNRTG	6 cut(s)	449, 850, 897, 1502, 2149, 2155
MspA1I	CMGCKG	4 cut(s)	118, 969, 1346, 2132
MspCI	CTTAAG	2 cut(s)	617, 1723
MspI	CCGG	4 cut(s)	147, 393, 918, 1802
MspR9I	CCNGG	2 cut(s)	919, 1802
MunI	CAATTG	1 cut(s)	1449
Mva1269I	GAATGC	1 cut(s)	1423
MvnI	CGCG	1 cut(s)	160
MwoI	GCNNNNNNNGC	8 cut(s)	75, 124, 1220, 1465, 1570, 1871, 1921, 2132
NciI	CCSGG	2 cut(s)	919, 1802
NdeI	CATATG	1 cut(s)	803
NdeII	GATC	9 cut(s)	750, 868, 1359, 1494, 1617, 1727, 2030, 2040, 2068
NlaIII	CATG	4 cut(s)	702, 1300, 1543, 2154
NlaIV	GGNNCC	6 cut(s)	752, 959, 960, 1024, 1658, 1799
NmuCI	GTSAC	2 cut(s)	562, 1246
NsbI	TGCGCA	1 cut(s)	1923
NspI	RCATGY	1 cut(s)	1543
PagI	TCATGA	1 cut(s)	1296
PciSI	GCTCTTC	2 cut(s)	66, 157
PcsI	WCGNNNNNNNCGW	2 cut(s)	170, 330
PctI	GAATGC	1 cut(s)	1423
PfeI	GAWTC	4 cut(s)	109, 861, 874, 881
PkrI	GCNGC	7 cut(s)	332, 799, 968, 1222, 1225, 1345, 2134
PleI	GAGTC	3 cut(s)	149, 1164, 1897
PpsI	GAGTC	3 cut(s)	149, 1164, 1897
PshAI	GACNNNNGTC	1 cut(s)	1715
PshBI	ATTAAT	1 cut(s)	1107
PsiI	TTATAA	1 cut(s)	360
PspN4I	GGNNCC	6 cut(s)	752, 959, 960, 1024, 1658, 1799
PspOMI	GGGCCC	1 cut(s)	957
PspPI	GGNCC	7 cut(s)	291, 920, 957, 958, 1023, 1089, 1657
PstI	CTGCAG	1 cut(s)	2137
PsuI	RGATCY	3 cut(s)	750, 1727, 2068
PvuII	CAGCTG	1 cut(s)	118
RsaI	GTAC	6 cut(s)	477, 663, 1268, 1680, 1764, 1889
RsaNI	GTAC	6 cut(s)	476, 662, 1267, 1679, 1763, 1888
RseI	CAYNNNNRTG	6 cut(s)	449, 850, 897, 1502, 2149, 2155
SalI	GTCGAC	2 cut(s)	323, 1313
SapI	GCTCTTC	2 cut(s)	66, 157
SaqAI	TTAA	8 cut(s)	470, 618, 1107, 1430, 1568, 1724, 1938, 1956
SatI	GCNGC	7 cut(s)	331, 798, 967, 1221, 1224, 1344, 2133
Sau3AI	GATC	9 cut(s)	750, 868, 1359, 1494, 1617, 1727, 2030, 2040, 2068
Sau96I	GGNCC	7 cut(s)	291, 920, 957, 958, 1023, 1089, 1657
ScaI	AGTACT	2 cut(s)	1764, 1889
SchI	GAGTC	3 cut(s)	150, 1165, 1897
ScrFI	CCNGG	2 cut(s)	919, 1802
SduI	GDGCHC	3 cut(s)	316, 961, 1802
SfaNI	GCATC	5 cut(s)	251, 1565, 1626, 2104, 2135
SfcI	CTRYAG	2 cut(s)	1792, 2133
SinI	GGWCC	3 cut(s)	291, 1089, 1657
SmiMI	CAYNNNNRTG	6 cut(s)	449, 850, 897, 1502, 2149, 2155
SmlI	CTYRAG	4 cut(s)	617, 1151, 1403, 1723
SmoI	CTYRAG	4 cut(s)	617, 1151, 1403, 1723
SpeI	ACTAGT	1 cut(s)	1199
Sse9I	AATT	9 cut(s)	226, 512, 789, 817, 988, 1077, 1449, 1756, 1813
SspI	AATATT	1 cut(s)	2061
SspMI	CTAG	5 cut(s)	747, 755, 1200, 1544, 1770
StyD4I	CCNGG	2 cut(s)	917, 1800
StyI	CCWWGG	1 cut(s)	754
TaaI	ACNGT	5 cut(s)	318, 475, 1606, 1661, 1717
TaiI	ACGT	3 cut(s)	485, 1839, 1956
TaqI	TCGA	5 cut(s)	173, 185, 324, 714, 1314
TaqII	GACCGA	1 cut(s)	52
TasI	AATT	9 cut(s)	226, 512, 789, 817, 988, 1077, 1449, 1756, 1813
TatI	WGTACW	5 cut(s)	661, 1266, 1678, 1762, 1887
TauI	GCSGC	3 cut(s)	333, 800, 1226
TfiI	GAWTC	4 cut(s)	109, 861, 874, 881
Tru1I	TTAA	8 cut(s)	470, 618, 1107, 1430, 1568, 1724, 1938, 1956
Tru9I	TTAA	8 cut(s)	470, 618, 1107, 1430, 1568, 1724, 1938, 1956
TscAI	CASTG	4 cut(s)	137, 933, 1422, 1916
TseFI	GTSAC	2 cut(s)	562, 1246
TseI	GCWGC	4 cut(s)	966, 1220, 1343, 2132
Tsp45I	GTSAC	2 cut(s)	562, 1246
TspDTI	ATGAA	8 cut(s)	834, 881, 1032, 1079, 1285, 1313, 1715, 2096
TspGWI	ACGGA	2 cut(s)	137, 735
TspRI	CASTG	4 cut(s)	137, 933, 1422, 1916
Vha464I	CTTAAG	2 cut(s)	617, 1723
VpaK11BI	GGWCC	3 cut(s)	291, 1089, 1657
VspI	ATTAAT	1 cut(s)	1107
XapI	RAATTY	4 cut(s)	817, 988, 1077, 1756
XceI	RCATGY	1 cut(s)	1543
XmaJI	CCTAGG	1 cut(s)	754
XmiI	GTMKAC	2 cut(s)	324, 1314
XspI	CTAG	5 cut(s)	747, 755, 1200, 1544, 1770
ZrmI	AGTACT	2 cut(s)	1764, 1889

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.