Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0003680

Physical Location & Seq

Genomic Coordinates Forward (+)

7087317 .. 7090686

Gene Structure 3370 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG090900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2286 bp

ATGGAAGACAAGAAAGCAGCACCAAACGACGTCGTTGTGCAGATTTCCGGCGCCGAAGACTCCACCTTTATGTTTTCGCCGCAGCCTTCCCGAACTGGGTCTCCTCAAAAAGGGTTCCAAGGCTCCAACTTTGAGCTAACGGAGCTCAGAGTCGCCGCCGGCTCTGCTTCGCCGGAGATCACAGCCACCACCGCCGGCAAGCCGCCGAGAATTCCCGGCGAGCTTCGCCGGGTTTCGCTTGCCAGGTCGGCGTTCTCCAAGCCCAAGTCGAGGTTCCAGGAGCCAGCTTACGTTAATGAAGTCAAGGTTGCTGAAGAAATTCCCAAGAGAAAGTCGAGCGCAAATTCACCCAATGTGGCTTCACCAAGCACTAAAGCCGCAGCCACTACTCCCAGAGAAGGTGTAAGATCAAACCCTATAACGCCGAGAACGCCGTTGATCGGAACACCGGCGCGTGGAGAAGAGGATGAGGAAGATGATGAAGAGGTGTACAAGACAGCCAATCTCAAGGTGCACCAGAAATCGGGTAAGAAGCTCAAAAAGTTGGTGTTACTTGAGTGGACTGTGTTTGTGTGCATTGTTGGGTTTTTAGTTGCTTGCTTAACTGTTGATAAGTTGGAGAGTAAGAATGTTTGGTCATTGGCACTGTGGAAATGGTGCGTGCTAGTGTTGGTTGTGTTGTGTGGTAGGTTGGTTACAGAATGGCTTATTAACATTTTGGTGTTCGTGATAGAAACGAATTACTTGCTTAAGAAGAAGGTTTTGTATTTTGTTTATGGGTTGAAGAGGAGTGTGCAGGTGTTTATTTGGTTGGGTTTGATTCTACTAGCATGGGGTTTGTTGTTTGATCATGGAGTGAAGAGGTCGGAGAACACGTCTAAGATTCTAGGTTATGTGACAAGGGGTTTGGCTTCTTGTGAGATTGGAGCAGCCATATGGCTGCTGAAGAATTTGTTAGTGAAGCTTTTAGCCACTTCTTTTCAGTCCACCAGGTTCTTTGATAGGATTAAAGAATCAATCTTTCATCAGTATGTTCTCCGCAGCCTATCGGGGCCTCCATTGGTGGAGATGAAATTTGGGAAGGAAACAAGTAGTGGCCGGTTGAGTTTCAAAAATCTGCCAAAAGCTAAAAATGATAGGAAGGAAGGGCCCAAAGAAGAAGTGATTGACGTAGAAAAACTTTCAAAAATGAAGCAGGATAAAATTTCTGCTTGGACGATGAAAGGGTTGATTAATGTGATAAGTGGTTCTGGGTTGTCTACCATCTCCCACACACTAGACGACATTAACGATGACGAGGGTGAGCAGAGGGATATAGAGATTACTAGTGAGTTTCAAGCACAGGCTGCAGCTTATGACATTTTCCTGAATGTAGCCAAGCGTGGTAGCAAGTACATTGAGGAAGATGACCTCTGTCGCTTCTTGAAAAAGGACGAAGTCGAGCTCGTATTTCCTCTGTTTGAAGGTGGAGCAGAAAGTCGAAAGATAAAAAAAAAGCCTTTGAAGAACTGGCTGGTGAATGTTTACAACGAACGCAAGTCATTGGCACATTCCTTAACTGATACTAAAACAGCTATAGAGGAGTTGAATAAGCTTGGCTCAGCTGTTGTGCTTGTTGTGATCCTTATCGTGTGGTTACTTCTGATGGGATTTTTGACAACCAACATACTGGTCTTTATATCATCACAGCTTTTACTGGTGGTGTTTGTTTTTGGTAACACTGCTAAAACTGTGTTTGAAGCCATCATATTTGTCTTTGTGATGCACCCATTTGATGTAGGGGACCGTTGTGTTGTTGATGGAGTACAGTTGATTGTTGAAGAGATGAACATATTGACAACGATCTTCTTGAAACCTGACAATGAGAAGATATACTATCCAAATTCAGTACTGGCTACTAAACCCATCAGCAACTTTTATAGGAGCCCGGAGATGGGTGATGCTGTTGAGTTTGCTGTTGATGTTTCTACAACAATTGACACTATTGCGAGCATGAAAGCCAGAATAAAAACGTACTTGGAGAGCAAGCCTCAGCACTGGCGTCCTGCCCATAGTGTGGCGGTGAAGGAAATTGAAGACGTAAACAAGATGACAATGGCTCTTTATATTTCTCATACTATAAATTTCCAGAACTATGGAGACAAAACCAGCCGCCGAACAGACCTAATCTTAGAGCTGAAGAAAATATTTGAAGAGCTTAATATAAAATATCACCTCCTGCCTCAGGAGATTTATCTCACCAAAGTTGGGTCAGCAACTACAGAGCTTCCACCCTCATGGCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel PsbX

Protein Analysis

761

Amino Acids

85.5

Weight (kDa)

8.94

Isoelectric Point (pI)

40.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	583 - 639	4e-10	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003680)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g08260 FvH4_1g08260 FvH4_1g08260 FvH4_1g08260
malus_domestica	MD02G1086900.v1.1 MD02G1087000.v1.1 MD15G1214500.v1.1 MD15G1214600.v1.1
prunus_persica	Prupe.7G202700_v2.0.a1
pyrus_communis	pycom02g06990 pycom02g07000 pycom15g19040
rosa_chinensis	RchiOBHm_Chr2g0094331
rosa_laevigata	RLG00000016480
rosa_multiflora	Rmu_sc0003819.1_g000016 Rmu_sc0025410.1_g000001 Rmu_sc0025411.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147230 Rroxscaffold_7G00185070
rosa_rugosa	Rorug02G0043300 Rorug02G0043300
rosa_samantha	Rh2AG091000 Rh2AG091100 Rh2BG090900 Rh2CG093400 Rh2CG093500 Rh2DG089900 Rh6AG505100 Rh6BG272500 Rh6CG273600 Rh6CG521500 Rh6CG521800 Rh6DG266600 Rh6DG507600
rosa_wichuraiana	Rw6G043950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	787
AatII	GACGTC	1 cut(s)	33
Acc36I	ACCTGC	1 cut(s)	787
AccB1I	GGYRCC	1 cut(s)	50
AccB7I	CCANNNNNTGG	1 cut(s)	2056
AccI	GTMKAC	1 cut(s)	1259
AccII	CGCG	1 cut(s)	454
AciI	CCGC	8 cut(s)	80, 156, 192, 203, 378, 1039, 2060, 2152
AclWI	GGATC	1 cut(s)	1615
AcoI	YGGCCR	1 cut(s)	1096
AcsI	RAATTY	8 cut(s)	210, 318, 343, 949, 1073, 1203, 1882, 2122
AcuI	CTGAAG	3 cut(s)	333, 965, 2198
AcyI	GRCGYC	3 cut(s)	30, 51, 2041
AfaI	GTAC	5 cut(s)	491, 1394, 1806, 1890, 2015
AflII	CTTAAG	1 cut(s)	749
AflIII	ACRYGT	1 cut(s)	873
AhlI	ACTAGT	1 cut(s)	1325
AjiI	CACGTC	1 cut(s)	876
AjnI	CCWGG	3 cut(s)	242, 276, 989
AjuI	GAANNNNNNNTTGG	2 cut(s)	257, 289
AloI	GAACNNNNNNTCC	2 cut(s)	85, 117
Alw21I	GWGCWC	3 cut(s)	147, 516, 1446
Alw26I	GTCTC	2 cut(s)	105, 2133
Alw44I	GTGCAC	1 cut(s)	512
AlwI	GGATC	1 cut(s)	1615
AoxI	GGCC	3 cut(s)	1052, 1096, 1148
ApaI	GGGCCC	1 cut(s)	1152
ApaLI	GTGCAC	1 cut(s)	512
ApeKI	GCWGC	8 cut(s)	17, 82, 380, 929, 940, 1041, 1346, 1349
ApoI	RAATTY	8 cut(s)	210, 318, 343, 949, 1073, 1203, 1882, 2122
ArsI	GACNNNNNNTTYG	2 cut(s)	889, 921
AseI	ATTAAT	1 cut(s)	1233
Asp700I	GAANNNNTTC	1 cut(s)	318
AspLEI	GCGC	4 cut(s)	53, 341, 454, 2283
AspS9I	GGNCC	4 cut(s)	1052, 1148, 1149, 1783
AsuC2I	CCSGG	3 cut(s)	216, 230, 1928
AsuHPI	GGTGA	8 cut(s)	339, 354, 1313, 1528, 1949, 2074, 2204, 2230
AvaII	GGWCC	1 cut(s)	1783
AxyI	CCTNAGG	1 cut(s)	2223
BaeGI	GKGCMC	2 cut(s)	516, 1152
BanI	GGYRCC	1 cut(s)	50
BanII	GRGCYC	4 cut(s)	147, 1152, 1446, 1928
BbsI	GAAGAC	3 cut(s)	12, 63, 2082
Bbv12I	GWGCWC	3 cut(s)	147, 516, 1446
BbvCI	CCTCAGC	1 cut(s)	2031
BbvI	GCAGC	8 cut(s)	29, 94, 392, 927, 941, 1053, 1333, 1361
BccI	CCATC	6 cut(s)	1271, 1639, 1751, 1793, 1913, 1927
BceAI	ACGGC	1 cut(s)	418
BcgI	CGANNNNNNTGC	2 cut(s)	727, 761
BciT130I	CCWGG	3 cut(s)	244, 278, 991
BclI	TGATCA	1 cut(s)	847
BcnI	CCSGG	3 cut(s)	216, 230, 1928
BcoDI	GTCTC	2 cut(s)	105, 2133
BcuI	ACTAGT	1 cut(s)	1325
BfaI	CTAG	5 cut(s)	665, 827, 887, 1277, 1326
BfmI	CTRYAG	3 cut(s)	1347, 1575, 2259
BfoI	RGCGCY	2 cut(s)	54, 2284
BfrI	CTTAAG	1 cut(s)	749
BfuAI	ACCTGC	1 cut(s)	787
BglI	GCCNNNNNGGC	1 cut(s)	248
BlpI	GCTNAGC	1 cut(s)	1600
BmcAI	AGTACT	1 cut(s)	1890
Bme1390I	CCNGG	6 cut(s)	216, 230, 244, 278, 991, 1928
Bme18I	GGWCC	1 cut(s)	1783
BmgBI	CACGTC	1 cut(s)	876
BmgT120I	GGNCC	4 cut(s)	1052, 1148, 1149, 1783
BmiI	GGNNCC	9 cut(s)	52, 116, 124, 275, 282, 1053, 1150, 1784, 1925
BmrFI	CCNGG	6 cut(s)	216, 230, 244, 278, 991, 1928
BmrI	ACTGGG	1 cut(s)	105
BmsI	GCATC	2 cut(s)	1752, 1930
BmuI	ACTGGG	1 cut(s)	105
BoxI	GACNNNNGTC	1 cut(s)	1413
BpiI	GAAGAC	3 cut(s)	12, 63, 2082
BplI	GAGNNNNNCTC	2 cut(s)	2014, 2046
Bpu10I	CCTNAGC	1 cut(s)	2031
Bpu1102I	GCTNAGC	1 cut(s)	1600
BpuEI	CTTGAG	2 cut(s)	491, 575
BpuMI	CCSGG	3 cut(s)	216, 230, 1928
BsaBI	GATNNNNATC	1 cut(s)	1625
BsaHI	GRCGYC	3 cut(s)	30, 51, 2041
BsaI	GGTCTC	1 cut(s)	105
BsaJI	CCNNGG	1 cut(s)	118
BsaXI	ACNNNNNCTCC	4 cut(s)	85, 115, 860, 890
Bse118I	RCCGGY	4 cut(s)	158, 194, 448, 1098
Bse1I	ACTGG	6 cut(s)	100, 1514, 1674, 1701, 1896, 2042
Bse21I	CCTNAGG	1 cut(s)	2223
Bse8I	GATNNNNATC	1 cut(s)	1625
BseBI	CCWGG	3 cut(s)	244, 278, 991
BseDI	CCNNGG	1 cut(s)	118
BseGI	GGATG	1 cut(s)	472
BseJI	GATNNNNATC	1 cut(s)	1625
BseMII	CTCAG	4 cut(s)	160, 1614, 2045, 2237
BseNI	ACTGG	6 cut(s)	100, 1514, 1674, 1701, 1896, 2042
BseRI	GAGGAG	3 cut(s)	93, 802, 1595
BseSI	GKGCMC	2 cut(s)	516, 1152
BseXI	GCAGC	8 cut(s)	29, 94, 392, 927, 941, 1053, 1333, 1361
BsgI	GTGCAG	2 cut(s)	59, 815
Bsh1236I	CGCG	1 cut(s)	454
BshFI	GGCC	3 cut(s)	1054, 1098, 1150
BshNI	GGYRCC	1 cut(s)	50
BsiHKAI	GWGCWC	3 cut(s)	147, 516, 1446
BsiSI	CCGG	9 cut(s)	48, 159, 173, 195, 216, 229, 449, 1099, 1928
BslFI	GGGAC	1 cut(s)	1796
BsmAI	GTCTC	2 cut(s)	105, 2133
BsmFI	GGGAC	1 cut(s)	1796
BsnI	GGCC	3 cut(s)	1054, 1098, 1150
Bso31I	GGTCTC	1 cut(s)	105
Bsp120I	GGGCCC	1 cut(s)	1148
Bsp1286I	GDGCHC	5 cut(s)	147, 516, 1152, 1446, 1928
Bsp1407I	TGTACA	1 cut(s)	489
Bsp143I	GATC	6 cut(s)	177, 407, 438, 847, 1620, 1842
Bsp1720I	GCTNAGC	1 cut(s)	1600
BspACI	CCGC	8 cut(s)	80, 156, 192, 203, 378, 1039, 2060, 2152
BspANI	GGCC	3 cut(s)	1054, 1098, 1150
BspCNI	CTCAG	4 cut(s)	159, 1613, 2044, 2236
BspFNI	CGCG	1 cut(s)	454
BspLI	GGNNCC	9 cut(s)	52, 116, 124, 275, 282, 1053, 1150, 1784, 1925
BspMAI	CTGCAG	1 cut(s)	1351
BspMI	ACCTGC	1 cut(s)	787
BspPI	GGATC	1 cut(s)	1615
BspQI	GCTCTTC	1 cut(s)	2187
BspT107I	GGYRCC	1 cut(s)	50
BspTI	CTTAAG	1 cut(s)	749
BspTNI	GGTCTC	1 cut(s)	105
BsrFI	RCCGGY	4 cut(s)	158, 194, 448, 1098
BsrGI	TGTACA	1 cut(s)	489
BsrI	ACTGG	6 cut(s)	100, 1514, 1674, 1701, 1896, 2042
BssAI	RCCGGY	4 cut(s)	158, 194, 448, 1098
BssECI	CCNNGG	1 cut(s)	118
BssMI	GATC	6 cut(s)	177, 407, 438, 847, 1620, 1842
BssNI	GRCGYC	3 cut(s)	30, 51, 2041
BssT1I	CCWWGG	1 cut(s)	118
Bst2UI	CCWGG	3 cut(s)	244, 278, 991
Bst4CI	ACNGT	6 cut(s)	565, 607, 648, 1732, 1787, 1809
Bst6I	CTCTTC	6 cut(s)	456, 477, 779, 854, 1815, 2187
BstACI	GRCGYC	3 cut(s)	30, 51, 2041
BstAFI	CTTAAG	1 cut(s)	749
BstAPI	GCANNNNNTGC	1 cut(s)	1346
BstAUI	TGTACA	1 cut(s)	489
BstDEI	CTNAG	6 cut(s)	146, 879, 1600, 2031, 2170, 2223
BstENI	CCTNNNNNAGG	2 cut(s)	108, 2222
BstF5I	GGATG	1 cut(s)	472
BstFNI	CGCG	1 cut(s)	454
BstH2I	RGCGCY	2 cut(s)	54, 2284
BstHHI	GCGC	4 cut(s)	53, 341, 454, 2283
BstKTI	GATC	6 cut(s)	180, 410, 441, 850, 1623, 1845
BstMAI	GTCTC	2 cut(s)	105, 2133
BstMBI	GATC	6 cut(s)	177, 407, 438, 847, 1620, 1842
BstMWI	GCNNNNNNNGC	7 cut(s)	142, 164, 191, 225, 248, 430, 1346
BstNI	CCWGG	3 cut(s)	244, 278, 991
BstPAI	GACNNNNGTC	1 cut(s)	1413
BstSCI	CCNGG	6 cut(s)	214, 228, 242, 276, 989, 1926
BstSFI	CTRYAG	3 cut(s)	1347, 1575, 2259
BstSLI	GKGCMC	2 cut(s)	516, 1152
BstUI	CGCG	1 cut(s)	454
BstV1I	GCAGC	8 cut(s)	29, 94, 392, 927, 941, 1053, 1333, 1361
BstV2I	GAAGAC	3 cut(s)	12, 63, 2082
BstXI	CCANNNNNNTGG	3 cut(s)	1669, 2135, 2277
Bsu36I	CCTNAGG	1 cut(s)	2223
BsuRI	GGCC	3 cut(s)	1054, 1098, 1150
BtrI	CACGTC	1 cut(s)	876
BtsCI	GGATG	1 cut(s)	472
BtsI	GCAGTG	1 cut(s)	1719
BtsIMutI	CAGTG	3 cut(s)	644, 1719, 2035
BveI	ACCTGC	1 cut(s)	787
CfoI	GCGC	4 cut(s)	53, 341, 454, 2283
Cfr10I	RCCGGY	4 cut(s)	158, 194, 448, 1098
Cfr13I	GGNCC	4 cut(s)	1052, 1148, 1149, 1783
CseI	GACGC	1 cut(s)	2030
CsiI	ACCWGGT	1 cut(s)	989
Csp6I	GTAC	5 cut(s)	490, 1393, 1805, 1889, 2014
CviAII	CATG	4 cut(s)	831, 851, 1993, 2277
CviQI	GTAC	5 cut(s)	490, 1393, 1805, 1889, 2014
DdeI	CTNAG	6 cut(s)	146, 879, 1600, 2031, 2170, 2223
DinI	GGCGCC	1 cut(s)	52
DpnI	GATC	6 cut(s)	179, 409, 440, 849, 1622, 1844
DpnII	GATC	6 cut(s)	177, 407, 438, 847, 1620, 1842
EaeI	YGGCCR	1 cut(s)	1096
Eam1104I	CTCTTC	6 cut(s)	456, 477, 779, 854, 1815, 2187
EarI	CTCTTC	6 cut(s)	456, 477, 779, 854, 1815, 2187
Ecl136II	GAGCTC	2 cut(s)	145, 1444
Eco130I	CCWWGG	1 cut(s)	118
Eco24I	GRGCYC	4 cut(s)	147, 1152, 1446, 1928
Eco31I	GGTCTC	1 cut(s)	105
Eco47I	GGWCC	1 cut(s)	1783
Eco53kI	GAGCTC	2 cut(s)	145, 1444
Eco57I	CTGAAG	3 cut(s)	333, 965, 2198
Eco81I	CCTNAGG	1 cut(s)	2223
EcoICRI	GAGCTC	2 cut(s)	145, 1444
EcoNI	CCTNNNNNAGG	2 cut(s)	108, 2222
EcoO109I	RGGNCCY	2 cut(s)	1052, 1148
EcoRI	GAATTC	1 cut(s)	210
EcoRII	CCWGG	3 cut(s)	242, 276, 989
EcoT14I	CCWWGG	1 cut(s)	118
EcoT38I	GRGCYC	4 cut(s)	147, 1152, 1446, 1928
EgeI	GGCGCC	1 cut(s)	52
EheI	GGCGCC	1 cut(s)	52
ErhI	CCWWGG	1 cut(s)	118
FaeI	CATG	4 cut(s)	834, 854, 1996, 2280
FaqI	GGGAC	1 cut(s)	1796
FatI	CATG	4 cut(s)	830, 850, 1992, 2276
FauNDI	CATATG	1 cut(s)	935
FbaI	TGATCA	1 cut(s)	847
FblI	GTMKAC	1 cut(s)	1259
FokI	GGATG	1 cut(s)	479
FriOI	GRGCYC	4 cut(s)	147, 1152, 1446, 1928
FspBI	CTAG	5 cut(s)	665, 827, 887, 1277, 1326
GlaI	GCGC	4 cut(s)	52, 340, 453, 2282
HaeII	RGCGCY	2 cut(s)	54, 2284
HaeIII	GGCC	3 cut(s)	1054, 1098, 1150
HapII	CCGG	9 cut(s)	48, 159, 173, 195, 216, 229, 449, 1099, 1928
HgaI	GACGC	1 cut(s)	2030
HhaI	GCGC	4 cut(s)	53, 341, 454, 2283
Hin1I	GRCGYC	3 cut(s)	30, 51, 2041
Hin1II	CATG	4 cut(s)	834, 854, 1996, 2280
Hin6I	GCGC	4 cut(s)	51, 339, 452, 2281
HinP1I	GCGC	4 cut(s)	51, 339, 452, 2281
HindIII	AAGCTT	2 cut(s)	962, 1592
HinfI	GANTC	5 cut(s)	59, 150, 820, 883, 1013
HpaII	CCGG	9 cut(s)	48, 159, 173, 195, 216, 229, 449, 1099, 1928
HphI	GGTGA	8 cut(s)	339, 354, 1313, 1528, 1949, 2074, 2204, 2230
Hpy166II	GTNNAC	7 cut(s)	490, 514, 561, 987, 1260, 1525, 2083
Hpy188I	TCNGA	4 cut(s)	149, 443, 868, 1644
Hpy188III	TCNNGA	7 cut(s)	90, 727, 1366, 1423, 1849, 2128, 2225
Hpy8I	GTNNAC	7 cut(s)	490, 514, 561, 987, 1260, 1525, 2083
Hpy99I	CGWCG	2 cut(s)	32, 35
HpyAV	CCTTC	8 cut(s)	96, 392, 751, 1075, 1135, 1139, 1457, 2059
HpyCH4III	ACNGT	6 cut(s)	565, 607, 648, 1732, 1787, 1809
HpyCH4IV	ACGT	6 cut(s)	30, 291, 875, 1170, 2012, 2079
HpyCH4V	TGCA	6 cut(s)	40, 514, 576, 796, 1349, 1765
HpyF10VI	GCNNNNNNNGC	7 cut(s)	142, 164, 191, 225, 248, 430, 1346
HpyF3I	CTNAG	6 cut(s)	146, 879, 1600, 2031, 2170, 2223
HpySE526I	ACGT	6 cut(s)	30, 291, 875, 1170, 2012, 2079
Hsp92I	GRCGYC	3 cut(s)	30, 51, 2041
Hsp92II	CATG	4 cut(s)	834, 854, 1996, 2280
HspAI	GCGC	4 cut(s)	51, 339, 452, 2281
KasI	GGCGCC	1 cut(s)	50
KroI	GCCGGC	2 cut(s)	158, 194
KroNI	GCCGGC	2 cut(s)	160, 196
Ksp22I	TGATCA	1 cut(s)	847
Kzo9I	GATC	6 cut(s)	177, 407, 438, 847, 1620, 1842
LguI	GCTCTTC	1 cut(s)	2187
LmnI	GCTCC	6 cut(s)	128, 142, 280, 926, 1469, 1923
Lsp1109I	GCAGC	8 cut(s)	29, 94, 392, 927, 941, 1053, 1333, 1361
LweI	GCATC	2 cut(s)	1752, 1930
MabI	ACCWGGT	1 cut(s)	989
MaeI	CTAG	5 cut(s)	665, 827, 887, 1277, 1326
MaeII	ACGT	6 cut(s)	30, 291, 875, 1170, 2012, 2079
MaeIII	GTNAC	5 cut(s)	549, 694, 895, 1635, 1715
MalI	GATC	6 cut(s)	179, 409, 440, 849, 1622, 1844
MboI	GATC	6 cut(s)	177, 407, 438, 847, 1620, 1842
MfeI	CAATTG	1 cut(s)	1974
MhlI	GDGCHC	5 cut(s)	147, 516, 1152, 1446, 1928
Mly113I	GGCGCC	1 cut(s)	51
MlyI	GAGTC	2 cut(s)	53, 159
MmeI	TCCRAC	3 cut(s)	150, 597, 846
MroNI	GCCGGC	2 cut(s)	158, 194
MroXI	GAANNNNTTC	1 cut(s)	318
MseI	TTAA	9 cut(s)	294, 602, 711, 750, 1008, 1233, 1287, 1556, 2199
MslI	CAYNNNNRTG	4 cut(s)	68, 719, 1029, 2275
MspA1I	CMGCKG	1 cut(s)	1604
MspCI	CTTAAG	1 cut(s)	749
MspI	CCGG	9 cut(s)	48, 159, 173, 195, 216, 229, 449, 1099, 1928
MspR9I	CCNGG	6 cut(s)	216, 230, 244, 278, 991, 1928
MunI	CAATTG	1 cut(s)	1974
MvaI	CCWGG	3 cut(s)	244, 278, 991
MvnI	CGCG	1 cut(s)	454
MwoI	GCNNNNNNNGC	7 cut(s)	142, 164, 191, 225, 248, 430, 1346
NaeI	GCCGGC	2 cut(s)	160, 196
NarI	GGCGCC	1 cut(s)	51
NciI	CCSGG	3 cut(s)	216, 230, 1928
NdeI	CATATG	1 cut(s)	935
NdeII	GATC	6 cut(s)	177, 407, 438, 847, 1620, 1842
NgoMIV	GCCGGC	2 cut(s)	158, 194
NlaIII	CATG	4 cut(s)	834, 854, 1996, 2280
NlaIV	GGNNCC	9 cut(s)	52, 116, 124, 275, 282, 1053, 1150, 1784, 1925
NmeAIII	GCCGAG	2 cut(s)	231, 450
NmuCI	GTSAC	1 cut(s)	895
PaqCI	CACCTGC	1 cut(s)	787
PciSI	GCTCTTC	1 cut(s)	2187
PcsI	WCGNNNNNNNCGW	2 cut(s)	872, 1287
PdiI	GCCGGC	2 cut(s)	160, 196
PdmI	GAANNNNTTC	1 cut(s)	318
PfeI	GAWTC	3 cut(s)	820, 883, 1013
PflFI	GACNNNGTC	1 cut(s)	1436
PflMI	CCANNNNNTGG	1 cut(s)	2056
PfoI	TCCNGGA	1 cut(s)	276
PleI	GAGTC	2 cut(s)	53, 158
PluTI	GGCGCC	1 cut(s)	54
PpsI	GAGTC	2 cut(s)	53, 158
PshAI	GACNNNNGTC	1 cut(s)	1413
PshBI	ATTAAT	1 cut(s)	1233
Psp124BI	GAGCTC	2 cut(s)	147, 1446
Psp6I	CCWGG	3 cut(s)	242, 276, 989
PspGI	CCWGG	3 cut(s)	242, 276, 989
PspN4I	GGNNCC	9 cut(s)	52, 116, 124, 275, 282, 1053, 1150, 1784, 1925
PspOMI	GGGCCC	1 cut(s)	1148
PspPI	GGNCC	4 cut(s)	1052, 1148, 1149, 1783
PstI	CTGCAG	1 cut(s)	1351
PsyI	GACNNNGTC	1 cut(s)	1436
PvuII	CAGCTG	1 cut(s)	1604
RsaI	GTAC	5 cut(s)	491, 1394, 1806, 1890, 2015
RsaNI	GTAC	5 cut(s)	490, 1393, 1805, 1889, 2014
RseI	CAYNNNNRTG	4 cut(s)	68, 719, 1029, 2275
SacI	GAGCTC	2 cut(s)	147, 1446
SapI	GCTCTTC	1 cut(s)	2187
SaqAI	TTAA	9 cut(s)	294, 602, 711, 750, 1008, 1233, 1287, 1556, 2199
Sau3AI	GATC	6 cut(s)	177, 407, 438, 847, 1620, 1842
Sau96I	GGNCC	4 cut(s)	1052, 1148, 1149, 1783
ScaI	AGTACT	1 cut(s)	1890
SchI	GAGTC	2 cut(s)	53, 159
ScrFI	CCNGG	6 cut(s)	216, 230, 244, 278, 991, 1928
SduI	GDGCHC	5 cut(s)	147, 516, 1152, 1446, 1928
SexAI	ACCWGGT	1 cut(s)	989
SfaNI	GCATC	2 cut(s)	1752, 1930
SfcI	CTRYAG	3 cut(s)	1347, 1575, 2259
SfoI	GGCGCC	1 cut(s)	52
SgrAI	CRCCGGYG	1 cut(s)	448
SinI	GGWCC	1 cut(s)	1783
SmiMI	CAYNNNNRTG	4 cut(s)	68, 719, 1029, 2275
SmlI	CTYRAG	3 cut(s)	506, 554, 749
SmoI	CTYRAG	3 cut(s)	506, 554, 749
SpeI	ACTAGT	1 cut(s)	1325
SsiI	CCGC	8 cut(s)	80, 156, 192, 203, 378, 1039, 2060, 2152
SspDI	GGCGCC	1 cut(s)	50
SspI	AATATT	1 cut(s)	2187
SspMI	CTAG	5 cut(s)	665, 827, 887, 1277, 1326
SstI	GAGCTC	2 cut(s)	147, 1446
StyD4I	CCNGG	6 cut(s)	214, 228, 242, 276, 989, 1926
StyI	CCWWGG	1 cut(s)	118
TaaI	ACNGT	6 cut(s)	565, 607, 648, 1732, 1787, 1809
TaiI	ACGT	6 cut(s)	33, 294, 878, 1173, 2015, 2082
TaqI	TCGA	4 cut(s)	269, 335, 1440, 1480
TatI	WGTACW	4 cut(s)	489, 1392, 1804, 1888
TauI	GCSGC	5 cut(s)	82, 158, 205, 380, 2154
TfiI	GAWTC	3 cut(s)	820, 883, 1013
Tru1I	TTAA	9 cut(s)	294, 602, 711, 750, 1008, 1233, 1287, 1556, 2199
Tru9I	TTAA	9 cut(s)	294, 602, 711, 750, 1008, 1233, 1287, 1556, 2199
TscAI	CASTG	3 cut(s)	651, 1726, 2042
TseFI	GTSAC	1 cut(s)	895
TseI	GCWGC	8 cut(s)	17, 82, 380, 929, 940, 1041, 1346, 1349
Tsp45I	GTSAC	1 cut(s)	895
TspDTI	ATGAA	8 cut(s)	312, 495, 1013, 1085, 1205, 1235, 1841, 2009
TspGWI	ACGGA	1 cut(s)	155
TspRI	CASTG	3 cut(s)	651, 1726, 2042
Tth111I	GACNNNGTC	1 cut(s)	1436
Van91I	CCANNNNNTGG	1 cut(s)	2056
Vha464I	CTTAAG	1 cut(s)	749
VneI	GTGCAC	1 cut(s)	512
VpaK11BI	GGWCC	1 cut(s)	1783
VspI	ATTAAT	1 cut(s)	1233
XagI	CCTNNNNNAGG	2 cut(s)	108, 2222
XapI	RAATTY	8 cut(s)	210, 318, 343, 949, 1073, 1203, 1882, 2122
XmiI	GTMKAC	1 cut(s)	1259
XmnI	GAANNNNTTC	1 cut(s)	318
XspI	CTAG	5 cut(s)	665, 827, 887, 1277, 1326
ZraI	GACGTC	1 cut(s)	31
ZrmI	AGTACT	1 cut(s)	1890

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.