Rh2CG093400

Mechanosensitive ion channel protein 10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003680
Physical Location & Seq
Forward (+)
7652033 .. 7653427
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG093400.1

Sequence Viewer

Length: 1395 bp
ATGGAAGACAAGAAAGCAGCACCAAACGACGTCGTTGTGCAGATTTCCGGCGCCGAAGACTCCACCTTTATGTTTTCGCCGCAGCCTTCCCGAACTGGGTCTCCTCAAAAAGGGTTCCAAGGCTCCAACTTTGAGCTAACGGAGCTCAGAGTCGCCGCCGGCTCTGCTTCGCCGGAGATCACAGCCACCACCGCCGGCAAGCCGCCGAGAATTCCCGGCGAGCTTCGCCGGGTTTCGCTTGCCAGGTCGGCGTTCTCCAAGCCCAAGTCGAGGTTCCAGGAGCCAGCTTACGTTAATGAAGTCAAGGTTGCTGAAGAAATTCCCAAGAGAAAGTCGAGCGCAAATTCACCCAATGTGGCTTCACCAAGCACTAAAGCCGCAGCCACTACTCCCAGAGAAGGTGTAAGATCAAACCCTATAACGCCGAGAACGCCGTTGATCGGAACACCGGCGCGTGGAGAAGAGGATGAGGAAGATGATGAAGAGGTGTACAAGACAGCCAATCTCAAGGTGCACCAGAAATCGGGTAAGAAGCTCAAAAAGTTGGTGTTACTTGAGTGGACTGTGTTTGTGTGCATTGTTGGGTTTTTAGTTGCTTGCTTAACTGTTGATAAGTTGGAGAGTAAGAATGTTTGGTCATTGGCACTGTGGAAATGGTGCGTGCTAGTGTTGGTTGTGTTGTGTGGTAGGTTGGTTACAGAATGGCTTATTAACATTTTGGTGTTCGTGATAGAAACGAATTACTTGCTTAAGAAGAAGGTTTTGTATTTTGTTTATGGGTTGAAGAGGAGTGTGCAGGTGTTTATTTGGTTGGGTTTGATTCTACTAGCATGGGGTTTGTTGTTTGATCATGGAGTGAAGAGGTCGGAGAACACGTCTAAGATTCTAGGTTATGTGACAAGGGGTTTGGCTTCTTGTGAGATTGGAGCAGCCATATGGCTGCTGAAGAATTTGTTAGTGAAGCTTTTAGCCACTTCTTTTCAGTCCACCAGGTTCTTTGATAGGATTAAAGAATCAATCTTTCATCAGTATGTTCTCCGCAGCCTATCGGGGCCTCCATTGGTGGAGATGAAATTTGGGAAGGAAACAAGTAGTGGCCGGTTGAGTTTCAAAAATCTGCCAAAAGCTAAAAATGATAGGAAGGAAGGGCCCAAAGAAGAAGTGATTGACGTAGAAAAACTTTCAAAAATGAAGCAGGATAAAATTTCTGCTTGGACGATGAAAGGGTTGATTAATGTGATAAGTGGTTCTGGGTTGTCTACCATCTCCCACACACTAGACGACATTAACGATGACGAGGGTGAGCAGAGGGATATAGAGATTACTAGTGAGTTTCAAGCACAGGCTGCAGCTTATGACATTTTCCTGAATGTAGCCAAGCGTGGTAGCAAGTGA

Protein Analysis

464

Amino Acids

51.52

Weight (kDa)

9.36

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Msy1 PF25886 186 - 327 6.5e-06 Mechanosensitive ion channel protein Msy1-like, transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 787
AatII GACGTC 1 cut(s) 33
Acc36I ACCTGC 1 cut(s) 787
AccB1I GGYRCC 1 cut(s) 50
AccI GTMKAC 1 cut(s) 1259
AccII CGCG 1 cut(s) 454
AciI CCGC 6 cut(s) 80, 156, 192, 203, 378, 1039
AcoI YGGCCR 1 cut(s) 1096
AcsI RAATTY 6 cut(s) 210, 318, 343, 949, 1073, 1203
AcuI CTGAAG 2 cut(s) 333, 965
AcyI GRCGYC 2 cut(s) 30, 51
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 7 cut(s) 96, 110, 270, 398, 440, 455, 523
AflII CTTAAG 1 cut(s) 749
AflIII ACRYGT 1 cut(s) 873
AgsI TTSAA 4 cut(s) 784, 1111, 1185, 1337
AhlI ACTAGT 1 cut(s) 1325
AjiI CACGTC 1 cut(s) 876
AjnI CCWGG 3 cut(s) 242, 276, 989
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AloI GAACNNNNNNTCC 2 cut(s) 85, 117
AluBI AGCT 8 cut(s) 136, 145, 223, 287, 535, 964, 1127, 1352
AluI AGCT 8 cut(s) 136, 145, 223, 287, 535, 964, 1127, 1352
Alw21I GWGCWC 2 cut(s) 147, 516
Alw26I GTCTC 1 cut(s) 105
Alw44I GTGCAC 1 cut(s) 512
AoxI GGCC 3 cut(s) 1052, 1096, 1148
ApaI GGGCCC 1 cut(s) 1152
ApaLI GTGCAC 1 cut(s) 512
ApeKI GCWGC 8 cut(s) 17, 82, 380, 929, 940, 1041, 1346, 1349
ApoI RAATTY 6 cut(s) 210, 318, 343, 949, 1073, 1203
ArsI GACNNNNNNTTYG 2 cut(s) 889, 921
AseI ATTAAT 1 cut(s) 1233
Asp700I GAANNNNTTC 1 cut(s) 318
AspLEI GCGC 3 cut(s) 53, 341, 454
AspS9I GGNCC 3 cut(s) 1052, 1148, 1149
AsuC2I CCSGG 2 cut(s) 216, 230
AsuHPI GGTGA 3 cut(s) 339, 354, 1313
BaeGI GKGCMC 2 cut(s) 516, 1152
BanI GGYRCC 1 cut(s) 50
BanII GRGCYC 2 cut(s) 147, 1152
BbsI GAAGAC 2 cut(s) 12, 63
Bbv12I GWGCWC 2 cut(s) 147, 516
BbvI GCAGC 8 cut(s) 29, 94, 392, 927, 941, 1053, 1333, 1361
BccI CCATC 1 cut(s) 1271
BceAI ACGGC 1 cut(s) 418
BcgI CGANNNNNNTGC 2 cut(s) 727, 761
BciT130I CCWGG 3 cut(s) 244, 278, 991
BclI TGATCA 1 cut(s) 847
BcnI CCSGG 2 cut(s) 216, 230
BcoDI GTCTC 1 cut(s) 105
BcuI ACTAGT 1 cut(s) 1325
BfaI CTAG 5 cut(s) 665, 827, 887, 1277, 1326
BfmI CTRYAG 1 cut(s) 1347
BfoI RGCGCY 1 cut(s) 54
BfrI CTTAAG 1 cut(s) 749
BfuAI ACCTGC 1 cut(s) 787
BglI GCCNNNNNGGC 1 cut(s) 248
Bme1390I CCNGG 5 cut(s) 216, 230, 244, 278, 991
BmgBI CACGTC 1 cut(s) 876
BmgT120I GGNCC 3 cut(s) 1052, 1148, 1149
BmiI GGNNCC 7 cut(s) 52, 116, 124, 275, 282, 1053, 1150
BmrFI CCNGG 5 cut(s) 216, 230, 244, 278, 991
BmrI ACTGGG 1 cut(s) 105
BmuI ACTGGG 1 cut(s) 105
BpiI GAAGAC 2 cut(s) 12, 63
BpuEI CTTGAG 2 cut(s) 491, 575
BpuMI CCSGG 2 cut(s) 216, 230
BsaHI GRCGYC 2 cut(s) 30, 51
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 118
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 860, 890
Bsc4I CCNNNNNNNGG 7 cut(s) 96, 110, 270, 398, 440, 455, 523
Bse118I RCCGGY 4 cut(s) 158, 194, 448, 1098
Bse1I ACTGG 1 cut(s) 100
BseBI CCWGG 3 cut(s) 244, 278, 991
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 7 cut(s) 96, 110, 270, 398, 440, 455, 523
BseMII CTCAG 1 cut(s) 160
BseNI ACTGG 1 cut(s) 100
BseRI GAGGAG 2 cut(s) 93, 802
BseSI GKGCMC 2 cut(s) 516, 1152
BseXI GCAGC 8 cut(s) 29, 94, 392, 927, 941, 1053, 1333, 1361
BsgI GTGCAG 2 cut(s) 59, 815
Bsh1236I CGCG 1 cut(s) 454
BshFI GGCC 3 cut(s) 1054, 1098, 1150
BshNI GGYRCC 1 cut(s) 50
BsiHKAI GWGCWC 2 cut(s) 147, 516
BsiSI CCGG 8 cut(s) 48, 159, 173, 195, 216, 229, 449, 1099
BslI CCNNNNNNNGG 7 cut(s) 96, 110, 270, 398, 440, 455, 523
BsmAI GTCTC 1 cut(s) 105
BsnI GGCC 3 cut(s) 1054, 1098, 1150
Bso31I GGTCTC 1 cut(s) 105
Bsp120I GGGCCC 1 cut(s) 1148
Bsp1286I GDGCHC 3 cut(s) 147, 516, 1152
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 4 cut(s) 177, 407, 438, 847
BspACI CCGC 6 cut(s) 80, 156, 192, 203, 378, 1039
BspANI GGCC 3 cut(s) 1054, 1098, 1150
BspCNI CTCAG 1 cut(s) 159
BspFNI CGCG 1 cut(s) 454
BspLI GGNNCC 7 cut(s) 52, 116, 124, 275, 282, 1053, 1150
BspMAI CTGCAG 1 cut(s) 1351
BspMI ACCTGC 1 cut(s) 787
BspT107I GGYRCC 1 cut(s) 50
BspTI CTTAAG 1 cut(s) 749
BspTNI GGTCTC 1 cut(s) 105
BsrFI RCCGGY 4 cut(s) 158, 194, 448, 1098
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 1 cut(s) 100
BssAI RCCGGY 4 cut(s) 158, 194, 448, 1098
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 4 cut(s) 177, 407, 438, 847
BssNI GRCGYC 2 cut(s) 30, 51
BssT1I CCWWGG 1 cut(s) 118
Bst2UI CCWGG 3 cut(s) 244, 278, 991
Bst4CI ACNGT 3 cut(s) 565, 607, 648
Bst6I CTCTTC 4 cut(s) 456, 477, 779, 854
BstACI GRCGYC 2 cut(s) 30, 51
BstAFI CTTAAG 1 cut(s) 749
BstAPI GCANNNNNTGC 1 cut(s) 1346
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 8 cut(s) 160, 196, 200, 221, 240, 285, 598, 662
BstDEI CTNAG 2 cut(s) 146, 879
BstENI CCTNNNNNAGG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 472
BstFNI CGCG 1 cut(s) 454
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 3 cut(s) 53, 341, 454
BstKTI GATC 4 cut(s) 180, 410, 441, 850
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 4 cut(s) 177, 407, 438, 847
BstMWI GCNNNNNNNGC 7 cut(s) 142, 164, 191, 225, 248, 430, 1346
BstNI CCWGG 3 cut(s) 244, 278, 991
BstSCI CCNGG 5 cut(s) 214, 228, 242, 276, 989
BstSFI CTRYAG 1 cut(s) 1347
BstSLI GKGCMC 2 cut(s) 516, 1152
BstUI CGCG 1 cut(s) 454
BstV1I GCAGC 8 cut(s) 29, 94, 392, 927, 941, 1053, 1333, 1361
BstV2I GAAGAC 2 cut(s) 12, 63
BsuRI GGCC 3 cut(s) 1054, 1098, 1150
BtrI CACGTC 1 cut(s) 876
BtsCI GGATG 1 cut(s) 472
BtsIMutI CAGTG 1 cut(s) 644
BveI ACCTGC 1 cut(s) 787
Cac8I GCNNGC 8 cut(s) 160, 196, 200, 221, 240, 285, 598, 662
CfoI GCGC 3 cut(s) 53, 341, 454
Cfr10I RCCGGY 4 cut(s) 158, 194, 448, 1098
Cfr13I GGNCC 3 cut(s) 1052, 1148, 1149
CsiI ACCWGGT 1 cut(s) 989
Csp6I GTAC 1 cut(s) 490
CviAII CATG 2 cut(s) 831, 851
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 2 cut(s) 146, 879
DinI GGCGCC 1 cut(s) 52
DpnI GATC 4 cut(s) 179, 409, 440, 849
DpnII GATC 4 cut(s) 177, 407, 438, 847
EaeI YGGCCR 1 cut(s) 1096
Eam1104I CTCTTC 4 cut(s) 456, 477, 779, 854
EarI CTCTTC 4 cut(s) 456, 477, 779, 854
Ecl136II GAGCTC 1 cut(s) 145
Eco130I CCWWGG 1 cut(s) 118
Eco24I GRGCYC 2 cut(s) 147, 1152
Eco31I GGTCTC 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 145
Eco57I CTGAAG 2 cut(s) 333, 965
EcoICRI GAGCTC 1 cut(s) 145
EcoNI CCTNNNNNAGG 1 cut(s) 108
EcoO109I RGGNCCY 2 cut(s) 1052, 1148
EcoRI GAATTC 1 cut(s) 210
EcoRII CCWGG 3 cut(s) 242, 276, 989
EcoT14I CCWWGG 1 cut(s) 118
EcoT38I GRGCYC 2 cut(s) 147, 1152
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 2 cut(s) 834, 854
FatI CATG 2 cut(s) 830, 850
FauNDI CATATG 1 cut(s) 935
FbaI TGATCA 1 cut(s) 847
FblI GTMKAC 1 cut(s) 1259
FokI GGATG 1 cut(s) 479
FriOI GRGCYC 2 cut(s) 147, 1152
FspBI CTAG 5 cut(s) 665, 827, 887, 1277, 1326
GlaI GCGC 3 cut(s) 52, 340, 453
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 3 cut(s) 1054, 1098, 1150
HapII CCGG 8 cut(s) 48, 159, 173, 195, 216, 229, 449, 1099
HhaI GCGC 3 cut(s) 53, 341, 454
Hin1I GRCGYC 2 cut(s) 30, 51
Hin1II CATG 2 cut(s) 834, 854
Hin6I GCGC 3 cut(s) 51, 339, 452
HinP1I GCGC 3 cut(s) 51, 339, 452
HindIII AAGCTT 1 cut(s) 962
HinfI GANTC 5 cut(s) 59, 150, 820, 883, 1013
HpaII CCGG 8 cut(s) 48, 159, 173, 195, 216, 229, 449, 1099
HphI GGTGA 3 cut(s) 339, 354, 1313
Hpy166II GTNNAC 5 cut(s) 490, 514, 561, 987, 1260
Hpy188I TCNGA 3 cut(s) 149, 443, 868
Hpy188III TCNNGA 3 cut(s) 90, 727, 1366
Hpy8I GTNNAC 5 cut(s) 490, 514, 561, 987, 1260
Hpy99I CGWCG 2 cut(s) 32, 35
HpyAV CCTTC 6 cut(s) 96, 392, 751, 1075, 1135, 1139
HpyCH4III ACNGT 3 cut(s) 565, 607, 648
HpyCH4IV ACGT 4 cut(s) 30, 291, 875, 1170
HpyCH4V TGCA 5 cut(s) 40, 514, 576, 796, 1349
HpyF10VI GCNNNNNNNGC 7 cut(s) 142, 164, 191, 225, 248, 430, 1346
HpyF3I CTNAG 2 cut(s) 146, 879
HpySE526I ACGT 4 cut(s) 30, 291, 875, 1170
Hsp92I GRCGYC 2 cut(s) 30, 51
Hsp92II CATG 2 cut(s) 834, 854
HspAI GCGC 3 cut(s) 51, 339, 452
KasI GGCGCC 1 cut(s) 50
KroI GCCGGC 2 cut(s) 158, 194
KroNI GCCGGC 2 cut(s) 160, 196
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 4 cut(s) 177, 407, 438, 847
LmnI GCTCC 4 cut(s) 128, 142, 280, 926
Lsp1109I GCAGC 8 cut(s) 29, 94, 392, 927, 941, 1053, 1333, 1361
MabI ACCWGGT 1 cut(s) 989
MaeI CTAG 5 cut(s) 665, 827, 887, 1277, 1326
MaeII ACGT 4 cut(s) 30, 291, 875, 1170
MaeIII GTNAC 3 cut(s) 549, 694, 895
MalI GATC 4 cut(s) 179, 409, 440, 849
MboI GATC 4 cut(s) 177, 407, 438, 847
MhlI GDGCHC 3 cut(s) 147, 516, 1152
MluCI AATT 7 cut(s) 210, 318, 343, 739, 949, 1073, 1203
Mly113I GGCGCC 1 cut(s) 51
MlyI GAGTC 2 cut(s) 53, 159
MmeI TCCRAC 3 cut(s) 150, 597, 846
MroNI GCCGGC 2 cut(s) 158, 194
MroXI GAANNNNTTC 1 cut(s) 318
MseI TTAA 7 cut(s) 294, 602, 711, 750, 1008, 1233, 1287
MslI CAYNNNNRTG 3 cut(s) 68, 719, 1029
MspCI CTTAAG 1 cut(s) 749
MspI CCGG 8 cut(s) 48, 159, 173, 195, 216, 229, 449, 1099
MspR9I CCNGG 5 cut(s) 216, 230, 244, 278, 991
MvaI CCWGG 3 cut(s) 244, 278, 991
MvnI CGCG 1 cut(s) 454
MwoI GCNNNNNNNGC 7 cut(s) 142, 164, 191, 225, 248, 430, 1346
NaeI GCCGGC 2 cut(s) 160, 196
NarI GGCGCC 1 cut(s) 51
NciI CCSGG 2 cut(s) 216, 230
NdeI CATATG 1 cut(s) 935
NdeII GATC 4 cut(s) 177, 407, 438, 847
NgoMIV GCCGGC 2 cut(s) 158, 194
NlaIII CATG 2 cut(s) 834, 854
NlaIV GGNNCC 7 cut(s) 52, 116, 124, 275, 282, 1053, 1150
NmeAIII GCCGAG 2 cut(s) 231, 450
NmuCI GTSAC 1 cut(s) 895
PaqCI CACCTGC 1 cut(s) 787
PcsI WCGNNNNNNNCGW 2 cut(s) 872, 1287
PdiI GCCGGC 2 cut(s) 160, 196
PdmI GAANNNNTTC 1 cut(s) 318
PfeI GAWTC 3 cut(s) 820, 883, 1013
PfoI TCCNGGA 1 cut(s) 276
PleI GAGTC 2 cut(s) 53, 158
PluTI GGCGCC 1 cut(s) 54
PpsI GAGTC 2 cut(s) 53, 158
PshBI ATTAAT 1 cut(s) 1233
Psp124BI GAGCTC 1 cut(s) 147
Psp6I CCWGG 3 cut(s) 242, 276, 989
PspGI CCWGG 3 cut(s) 242, 276, 989
PspN4I GGNNCC 7 cut(s) 52, 116, 124, 275, 282, 1053, 1150
PspOMI GGGCCC 1 cut(s) 1148
PspPI GGNCC 3 cut(s) 1052, 1148, 1149
PstI CTGCAG 1 cut(s) 1351
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
RseI CAYNNNNRTG 3 cut(s) 68, 719, 1029
SacI GAGCTC 1 cut(s) 147
SaqAI TTAA 7 cut(s) 294, 602, 711, 750, 1008, 1233, 1287
Sau3AI GATC 4 cut(s) 177, 407, 438, 847
Sau96I GGNCC 3 cut(s) 1052, 1148, 1149
SchI GAGTC 2 cut(s) 53, 159
ScrFI CCNGG 5 cut(s) 216, 230, 244, 278, 991
SduI GDGCHC 3 cut(s) 147, 516, 1152
SexAI ACCWGGT 1 cut(s) 989
SfcI CTRYAG 1 cut(s) 1347
SfoI GGCGCC 1 cut(s) 52
SgrAI CRCCGGYG 1 cut(s) 448
SmiMI CAYNNNNRTG 3 cut(s) 68, 719, 1029
SmlI CTYRAG 3 cut(s) 506, 554, 749
SmoI CTYRAG 3 cut(s) 506, 554, 749
SpeI ACTAGT 1 cut(s) 1325
Sse9I AATT 7 cut(s) 210, 318, 343, 739, 949, 1073, 1203
SsiI CCGC 6 cut(s) 80, 156, 192, 203, 378, 1039
SspDI GGCGCC 1 cut(s) 50
SspMI CTAG 5 cut(s) 665, 827, 887, 1277, 1326
SstI GAGCTC 1 cut(s) 147
StyD4I CCNGG 5 cut(s) 214, 228, 242, 276, 989
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 3 cut(s) 565, 607, 648
TaiI ACGT 4 cut(s) 33, 294, 878, 1173
TaqI TCGA 2 cut(s) 269, 335
TasI AATT 7 cut(s) 210, 318, 343, 739, 949, 1073, 1203
TatI WGTACW 1 cut(s) 489
TauI GCSGC 4 cut(s) 82, 158, 205, 380
TfiI GAWTC 3 cut(s) 820, 883, 1013
Tru1I TTAA 7 cut(s) 294, 602, 711, 750, 1008, 1233, 1287
Tru9I TTAA 7 cut(s) 294, 602, 711, 750, 1008, 1233, 1287
TscAI CASTG 1 cut(s) 651
TseFI GTSAC 1 cut(s) 895
TseI GCWGC 8 cut(s) 17, 82, 380, 929, 940, 1041, 1346, 1349
Tsp45I GTSAC 1 cut(s) 895
TspDTI ATGAA 6 cut(s) 312, 495, 1013, 1085, 1205, 1235
TspGWI ACGGA 1 cut(s) 155
TspRI CASTG 1 cut(s) 651
Vha464I CTTAAG 1 cut(s) 749
VneI GTGCAC 1 cut(s) 512
VspI ATTAAT 1 cut(s) 1233
XagI CCTNNNNNAGG 1 cut(s) 108
XapI RAATTY 6 cut(s) 210, 318, 343, 949, 1073, 1203
XmiI GTMKAC 1 cut(s) 1259
XmnI GAANNNNTTC 1 cut(s) 318
XspI CTAG 5 cut(s) 665, 827, 887, 1277, 1326
ZraI GACGTC 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.