Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0003680

Physical Location & Seq

Genomic Coordinates Forward (+)

6986507 .. 6989911

Gene Structure 3405 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016480

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2301 bp

ATGGAAGACAAGAAAGCAGCACCAAACGACGTCGTTGTGCAGATTTCCGGCACAGAAGACTCCACCTTTATGTTTTCGCCGCAGCCTTCCCGAACTGGGTCTCCTCAAAAAGGGTTCCAAGGCTCCAACTTTGAGCTAACGGAGCTCAGGGTCGCCGCCGGCGCCGGCTCTGCTTCGCCGGAGATTACAGCCACTACCGCCGGCAAGCCGCCTAGAATTCCCGGCGAGCTTCGGCGGGTTTCGCTTGCCAGGTCGGCGTTCTCCAAGCCCAAGTCGAGGTTCCAGGAGCCAACTTACATTAATGAAGTCAAGGTTGCTGAAGAAATTCCCAAGAGAAAGTCGAATGCAAATTCACCCAATGTGGCTTCACCAAGCACTAAAGCCACAGCCACTACTCCCAGAGAAGGTGCAAGATCAAACCCTATAACGCCGAGAACGCCGTTGATCGGAACACCGGCGCGTGGAGAAGAGGATGAGGAAGATGATGAAGAGGTGTATAAGACAGCCAATCTCAAGGTGCACCAGAAATCGGGTAAGAAGCTCAAAAAGTTGGTGTTACTTGAGTGGACTGTGTTTGTGTGCATTGTTGGGTTTTTAGTTGCTTGCTTAATTGTTGATAAGTTGGAGAGTAAGAATATTTGGTCATTGGAACTGTGGAAATGGTGCGTGCTAGTGTTGGTTGTGTTGTGTGGTAGGTTGGTTACAGAATGGCTTATTAATATTTTGGTTTTCGTGATAGAAACGAATTACTTGCTTAAGAAGAAGGTTTTGTATTTTGTTTATGGATTGAAGAGGAGTGTGCAGGTGTTTATTTGGTTGGGTTTGATTCTACTAGCATGGGGTTTGTTGTTTGATCATGGAGTGAAGAGGTCGAAGAACACGTCTAAGATTCTAGGTTATGTGACAAGGGGTTTGGCTTCTTGTGAGATTGGAGCAGCCATATGGCTGTTGAAGAATTTGTTAGTGAAGCTTTTAGCCACTTCTTTTCAGTCCACCAGGTTCTTTGATCGGATTAAAGAGTCGATCTTTCATCAGTATGTTCTCCGCAGCCTTTCGGGCCCTCCATTGGTGGAGATGGCTGGAAGAAAATTTGGAAAGGAATCAAGTAGTGGCCGGTTGAGTTTCAAAAATCTGCCAAAAGCTAAAAATGATGGGAAGGAAGGGCCCAAAGAAGAAGTGATTGACGTAGAAAAACTTTCGAAAATGAAGCAGGATAAAATTTCTGCTTGGACGATGAAAGGGTTGATTAATGTGATAAGTGGTTCTGGGTTGTCTACCATCTCCCACACACTAGACGACATTAACGATGAGGAGGGTGAGCAGAGGGATATAGAGATTACTAGTGAGTTTGAAGCACAGGCTGCAGCTTATGACATTTTCCTGAATGTAGCCAAGCGTGGTAGCAAGTACATTGAGGAAGATGACCTCTGTCGCTTCTTGAAAAAGGACGAAGTCGAGCTCGTATTTCCTCTGTTTGAAGGTGGAGCAGAAAGTCGAAAGATAAAAAAAAAGCCTTTGAAGAACTGGCTGGTGAATGTTTACAACGAACGCAAGTCATTGGCACATTCCTTAACTGATACTAAAACAGCTATAGAGGAGTTGAATAGGCTTGGCTCAGCAGTTGTGCTTGTTGTGATCCTTATCGTGTGGTTACTTCTGATGGGATTTTTGACAACCAACATACTGGTCTTTATATCATCACAGCTTTTACTGGTGGCGTTTGTTTTTGGTAACACTGCTAAAACTGTGTTTGAAGCCATCATATTTGTCTTTGTGATGCACCCATTTGATGTGGGGGACCGTTGTGTTGTTGATGGAGTACAGTTGATTGTTGAAGAGATGAACATATTGACAACGATCTTCTTGAAACCTGACAATGAGAAGATATACTATCCAAATTCAGTACTGGCTACTAAACCCATCAGCAACTTTTATAGGAGCCCGGAGATGGGTGATGCTGTTGAGTTCGCTGTTGATGTTTCTACAACAATTGACACTATTGCGAGCATGAAAGCCAGAATAAAAACATACTTGGAGAGCAAGCCTCAGCACTGGCGTCCTGCCCATAGTGTGGCGGTGAAGGAAATTGAAGACGTAAACAAGATGACAATGGCTCTTTATATTTCTCATACTATAAATTTCCAGAACTATGGAGACAAAACCAGCCGCCGAACAGACCTAATCTTAGAGCTGAAGAAAATATTTGAAGAGCTTAATATAAAATATCACCTCCTGCCTCAGGAGATTTATCTCACCAAAGTTGGGTCAGCAACTACAGAGCTTCCACCCTCATGGCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel PsbX

Protein Analysis

767

Amino Acids

86.0

Weight (kDa)

8.94

Isoelectric Point (pI)

41.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Msy1	PF25886	187 - 329	1.3e-06	Mechanosensitive ion channel protein Msy1-like, transmembrane domain
MS_channel_2nd	PF00924	588 - 644	4e-10	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003680)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g08260 FvH4_1g08260 FvH4_1g08260 FvH4_1g08260
malus_domestica	MD02G1086900.v1.1 MD02G1087000.v1.1 MD15G1214500.v1.1 MD15G1214600.v1.1
prunus_persica	Prupe.7G202700_v2.0.a1
pyrus_communis	pycom02g06990 pycom02g07000 pycom15g19040
rosa_chinensis	RchiOBHm_Chr2g0094331
rosa_laevigata	RLG00000016480
rosa_multiflora	Rmu_sc0003819.1_g000016 Rmu_sc0025410.1_g000001 Rmu_sc0025411.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147230 Rroxscaffold_7G00185070
rosa_rugosa	Rorug02G0043300 Rorug02G0043300
rosa_samantha	Rh2AG091000 Rh2AG091100 Rh2BG090900 Rh2CG093400 Rh2CG093500 Rh2DG089900 Rh6AG505100 Rh6BG272500 Rh6CG273600 Rh6CG521500 Rh6CG521800 Rh6DG266600 Rh6DG507600
rosa_wichuraiana	Rw6G043950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	793
AatII	GACGTC	1 cut(s)	33
Acc36I	ACCTGC	1 cut(s)	793
AccB1I	GGYRCC	1 cut(s)	161
AccB7I	CCANNNNNTGG	1 cut(s)	2071
AccI	GTMKAC	1 cut(s)	1274
AccII	CGCG	1 cut(s)	460
AciI	CCGC	8 cut(s)	80, 156, 198, 209, 235, 1045, 2075, 2167
AclWI	GGATC	1 cut(s)	1630
AcoI	YGGCCR	1 cut(s)	1111
AcsI	RAATTY	8 cut(s)	216, 324, 349, 955, 1088, 1218, 1897, 2137
AcuI	CTGAAG	2 cut(s)	339, 2213
AcyI	GRCGYC	3 cut(s)	30, 162, 2056
AfaI	GTAC	3 cut(s)	1409, 1821, 1905
AflII	CTTAAG	1 cut(s)	755
AflIII	ACRYGT	1 cut(s)	879
AhlI	ACTAGT	1 cut(s)	1340
AjiI	CACGTC	1 cut(s)	882
AjnI	CCWGG	3 cut(s)	248, 282, 995
AjuI	GAANNNNNNNTTGG	2 cut(s)	263, 295
AloI	GAACNNNNNNTCC	2 cut(s)	85, 117
Alw21I	GWGCWC	3 cut(s)	147, 522, 1461
Alw26I	GTCTC	2 cut(s)	105, 2148
Alw44I	GTGCAC	1 cut(s)	518
AlwI	GGATC	1 cut(s)	1630
AoxI	GGCC	3 cut(s)	1057, 1111, 1163
ApaI	GGGCCC	2 cut(s)	1061, 1167
ApaLI	GTGCAC	1 cut(s)	518
ApeKI	GCWGC	6 cut(s)	17, 82, 935, 1047, 1361, 1364
ApoI	RAATTY	8 cut(s)	216, 324, 349, 955, 1088, 1218, 1897, 2137
ArsI	GACNNNNNNTTYG	4 cut(s)	866, 895, 898, 927
AseI	ATTAAT	3 cut(s)	300, 717, 1248
Asp700I	GAANNNNTTC	1 cut(s)	324
AspLEI	GCGC	3 cut(s)	164, 460, 2298
AspS9I	GGNCC	5 cut(s)	1057, 1058, 1163, 1164, 1798
AsuC2I	CCSGG	2 cut(s)	222, 1943
AsuHPI	GGTGA	8 cut(s)	345, 360, 1328, 1543, 1964, 2089, 2219, 2245
AsuII	TTCGAA	1 cut(s)	1199
AvaII	GGWCC	1 cut(s)	1798
AxyI	CCTNAGG	1 cut(s)	2238
BaeGI	GKGCMC	3 cut(s)	522, 1061, 1167
BanI	GGYRCC	1 cut(s)	161
BanII	GRGCYC	5 cut(s)	147, 1061, 1167, 1461, 1943
BbsI	GAAGAC	3 cut(s)	12, 63, 2097
Bbv12I	GWGCWC	3 cut(s)	147, 522, 1461
BbvCI	CCTCAGC	1 cut(s)	2046
BbvI	GCAGC	6 cut(s)	29, 94, 947, 1059, 1348, 1376
BccI	CCATC	8 cut(s)	1069, 1145, 1286, 1654, 1766, 1808, 1928, 1942
BceAI	ACGGC	1 cut(s)	424
BcgI	CGANNNNNNTGC	2 cut(s)	733, 767
BciT130I	CCWGG	3 cut(s)	250, 284, 997
BclI	TGATCA	1 cut(s)	853
BcnI	CCSGG	2 cut(s)	222, 1943
BcoDI	GTCTC	2 cut(s)	105, 2148
BcuI	ACTAGT	1 cut(s)	1340
BfaI	CTAG	6 cut(s)	213, 671, 833, 893, 1292, 1341
BfmI	CTRYAG	3 cut(s)	1362, 1590, 2274
BfoI	RGCGCY	2 cut(s)	165, 2299
BfrI	CTTAAG	1 cut(s)	755
BfuAI	ACCTGC	1 cut(s)	793
BglI	GCCNNNNNGGC	2 cut(s)	254, 1056
BlpI	GCTNAGC	1 cut(s)	1615
BmcAI	AGTACT	1 cut(s)	1905
Bme1390I	CCNGG	5 cut(s)	222, 250, 284, 997, 1943
Bme18I	GGWCC	1 cut(s)	1798
BmgBI	CACGTC	1 cut(s)	882
BmgT120I	GGNCC	5 cut(s)	1057, 1058, 1163, 1164, 1798
BmiI	GGNNCC	9 cut(s)	116, 124, 163, 281, 288, 1059, 1165, 1799, 1940
BmrFI	CCNGG	5 cut(s)	222, 250, 284, 997, 1943
BmrI	ACTGGG	1 cut(s)	105
BmsI	GCATC	2 cut(s)	1767, 1945
BmuI	ACTGGG	1 cut(s)	105
BoxI	GACNNNNGTC	1 cut(s)	1428
BpiI	GAAGAC	3 cut(s)	12, 63, 2097
BplI	GAGNNNNNCTC	2 cut(s)	2029, 2061
Bpu10I	CCTNAGC	2 cut(s)	146, 2046
Bpu1102I	GCTNAGC	1 cut(s)	1615
Bpu14I	TTCGAA	1 cut(s)	1199
BpuEI	CTTGAG	2 cut(s)	497, 581
BpuMI	CCSGG	2 cut(s)	222, 1943
BsaBI	GATNNNNATC	1 cut(s)	1640
BsaHI	GRCGYC	3 cut(s)	30, 162, 2056
BsaI	GGTCTC	1 cut(s)	105
BsaJI	CCNNGG	1 cut(s)	118
BsaXI	ACNNNNNCTCC	2 cut(s)	85, 115
Bse118I	RCCGGY	5 cut(s)	158, 164, 200, 454, 1113
Bse1I	ACTGG	6 cut(s)	100, 1529, 1689, 1716, 1911, 2057
Bse21I	CCTNAGG	1 cut(s)	2238
Bse8I	GATNNNNATC	1 cut(s)	1640
BseBI	CCWGG	3 cut(s)	250, 284, 997
BseDI	CCNNGG	1 cut(s)	118
BseGI	GGATG	1 cut(s)	478
BseJI	GATNNNNATC	1 cut(s)	1640
BseMII	CTCAG	4 cut(s)	160, 1629, 2060, 2252
BseNI	ACTGG	6 cut(s)	100, 1529, 1689, 1716, 1911, 2057
BseRI	GAGGAG	4 cut(s)	93, 808, 1325, 1610
BseSI	GKGCMC	3 cut(s)	522, 1061, 1167
BseXI	GCAGC	6 cut(s)	29, 94, 947, 1059, 1348, 1376
BsgI	GTGCAG	2 cut(s)	59, 821
Bsh1236I	CGCG	1 cut(s)	460
BshFI	GGCC	3 cut(s)	1059, 1113, 1165
BshNI	GGYRCC	1 cut(s)	161
BsiHKAI	GWGCWC	3 cut(s)	147, 522, 1461
BsiSI	CCGG	9 cut(s)	48, 159, 165, 179, 201, 222, 455, 1114, 1943
BslFI	GGGAC	1 cut(s)	1811
BsmAI	GTCTC	2 cut(s)	105, 2148
BsmFI	GGGAC	1 cut(s)	1811
BsmI	GAATGC	1 cut(s)	349
BsnI	GGCC	3 cut(s)	1059, 1113, 1165
Bso31I	GGTCTC	1 cut(s)	105
Bsp119I	TTCGAA	1 cut(s)	1199
Bsp120I	GGGCCC	2 cut(s)	1057, 1163
Bsp1286I	GDGCHC	6 cut(s)	147, 522, 1061, 1167, 1461, 1943
Bsp143I	GATC	7 cut(s)	413, 444, 853, 1006, 1023, 1635, 1857
Bsp1720I	GCTNAGC	1 cut(s)	1615
BspACI	CCGC	8 cut(s)	80, 156, 198, 209, 235, 1045, 2075, 2167
BspANI	GGCC	3 cut(s)	1059, 1113, 1165
BspCNI	CTCAG	4 cut(s)	159, 1628, 2059, 2251
BspFNI	CGCG	1 cut(s)	460
BspLI	GGNNCC	9 cut(s)	116, 124, 163, 281, 288, 1059, 1165, 1799, 1940
BspMAI	CTGCAG	1 cut(s)	1366
BspMI	ACCTGC	1 cut(s)	793
BspPI	GGATC	1 cut(s)	1630
BspQI	GCTCTTC	1 cut(s)	2202
BspT104I	TTCGAA	1 cut(s)	1199
BspT107I	GGYRCC	1 cut(s)	161
BspTI	CTTAAG	1 cut(s)	755
BspTNI	GGTCTC	1 cut(s)	105
BsrFI	RCCGGY	5 cut(s)	158, 164, 200, 454, 1113
BsrI	ACTGG	6 cut(s)	100, 1529, 1689, 1716, 1911, 2057
BssAI	RCCGGY	5 cut(s)	158, 164, 200, 454, 1113
BssECI	CCNNGG	1 cut(s)	118
BssMI	GATC	7 cut(s)	413, 444, 853, 1006, 1023, 1635, 1857
BssNI	GRCGYC	3 cut(s)	30, 162, 2056
BssT1I	CCWWGG	1 cut(s)	118
Bst2UI	CCWGG	3 cut(s)	250, 284, 997
Bst4CI	ACNGT	5 cut(s)	571, 654, 1747, 1802, 1824
Bst6I	CTCTTC	6 cut(s)	462, 483, 785, 860, 1830, 2202
BstACI	GRCGYC	3 cut(s)	30, 162, 2056
BstAFI	CTTAAG	1 cut(s)	755
BstAPI	GCANNNNNTGC	1 cut(s)	1361
BstBI	TTCGAA	1 cut(s)	1199
BstDEI	CTNAG	6 cut(s)	146, 885, 1615, 2046, 2185, 2238
BstENI	CCTNNNNNAGG	2 cut(s)	108, 2237
BstF5I	GGATG	1 cut(s)	478
BstFNI	CGCG	1 cut(s)	460
BstH2I	RGCGCY	2 cut(s)	165, 2299
BstHHI	GCGC	3 cut(s)	164, 460, 2298
BstKTI	GATC	7 cut(s)	416, 447, 856, 1009, 1026, 1638, 1860
BstMAI	GTCTC	2 cut(s)	105, 2148
BstMBI	GATC	7 cut(s)	413, 444, 853, 1006, 1023, 1635, 1857
BstMWI	GCNNNNNNNGC	9 cut(s)	142, 161, 170, 197, 241, 254, 436, 1056, 1361
BstNI	CCWGG	3 cut(s)	250, 284, 997
BstPAI	GACNNNNGTC	1 cut(s)	1428
BstSCI	CCNGG	5 cut(s)	220, 248, 282, 995, 1941
BstSFI	CTRYAG	3 cut(s)	1362, 1590, 2274
BstSLI	GKGCMC	3 cut(s)	522, 1061, 1167
BstUI	CGCG	1 cut(s)	460
BstV1I	GCAGC	6 cut(s)	29, 94, 947, 1059, 1348, 1376
BstV2I	GAAGAC	3 cut(s)	12, 63, 2097
BstXI	CCANNNNNNTGG	3 cut(s)	1684, 2150, 2292
Bsu36I	CCTNAGG	1 cut(s)	2238
BsuRI	GGCC	3 cut(s)	1059, 1113, 1165
BtrI	CACGTC	1 cut(s)	882
BtsCI	GGATG	1 cut(s)	478
BtsI	GCAGTG	1 cut(s)	1734
BtsIMutI	CAGTG	2 cut(s)	1734, 2050
BveI	ACCTGC	1 cut(s)	793
CfoI	GCGC	3 cut(s)	164, 460, 2298
Cfr10I	RCCGGY	5 cut(s)	158, 164, 200, 454, 1113
Cfr13I	GGNCC	5 cut(s)	1057, 1058, 1163, 1164, 1798
CseI	GACGC	1 cut(s)	2045
CsiI	ACCWGGT	1 cut(s)	995
Csp6I	GTAC	3 cut(s)	1408, 1820, 1904
CviAII	CATG	4 cut(s)	837, 857, 2008, 2292
CviQI	GTAC	3 cut(s)	1408, 1820, 1904
DdeI	CTNAG	6 cut(s)	146, 885, 1615, 2046, 2185, 2238
DinI	GGCGCC	1 cut(s)	163
DpnI	GATC	7 cut(s)	415, 446, 855, 1008, 1025, 1637, 1859
DpnII	GATC	7 cut(s)	413, 444, 853, 1006, 1023, 1635, 1857
EaeI	YGGCCR	1 cut(s)	1111
Eam1104I	CTCTTC	6 cut(s)	462, 483, 785, 860, 1830, 2202
EarI	CTCTTC	6 cut(s)	462, 483, 785, 860, 1830, 2202
Ecl136II	GAGCTC	2 cut(s)	145, 1459
Eco130I	CCWWGG	1 cut(s)	118
Eco24I	GRGCYC	5 cut(s)	147, 1061, 1167, 1461, 1943
Eco31I	GGTCTC	1 cut(s)	105
Eco47I	GGWCC	1 cut(s)	1798
Eco53kI	GAGCTC	2 cut(s)	145, 1459
Eco57I	CTGAAG	2 cut(s)	339, 2213
Eco81I	CCTNAGG	1 cut(s)	2238
EcoICRI	GAGCTC	2 cut(s)	145, 1459
EcoNI	CCTNNNNNAGG	2 cut(s)	108, 2237
EcoO109I	RGGNCCY	2 cut(s)	1058, 1163
EcoRI	GAATTC	1 cut(s)	216
EcoRII	CCWGG	3 cut(s)	248, 282, 995
EcoT14I	CCWWGG	1 cut(s)	118
EcoT38I	GRGCYC	5 cut(s)	147, 1061, 1167, 1461, 1943
EgeI	GGCGCC	1 cut(s)	163
EheI	GGCGCC	1 cut(s)	163
ErhI	CCWWGG	1 cut(s)	118
FaeI	CATG	4 cut(s)	840, 860, 2011, 2295
FaqI	GGGAC	1 cut(s)	1811
FatI	CATG	4 cut(s)	836, 856, 2007, 2291
FauI	CCCGC	1 cut(s)	228
FauNDI	CATATG	1 cut(s)	941
FbaI	TGATCA	1 cut(s)	853
FblI	GTMKAC	1 cut(s)	1274
FokI	GGATG	1 cut(s)	485
FriOI	GRGCYC	5 cut(s)	147, 1061, 1167, 1461, 1943
FspBI	CTAG	6 cut(s)	213, 671, 833, 893, 1292, 1341
GlaI	GCGC	3 cut(s)	163, 459, 2297
HaeII	RGCGCY	2 cut(s)	165, 2299
HaeIII	GGCC	3 cut(s)	1059, 1113, 1165
HapII	CCGG	9 cut(s)	48, 159, 165, 179, 201, 222, 455, 1114, 1943
HgaI	GACGC	1 cut(s)	2045
HhaI	GCGC	3 cut(s)	164, 460, 2298
Hin1I	GRCGYC	3 cut(s)	30, 162, 2056
Hin1II	CATG	4 cut(s)	840, 860, 2011, 2295
Hin6I	GCGC	3 cut(s)	162, 458, 2296
HinP1I	GCGC	3 cut(s)	162, 458, 2296
HindIII	AAGCTT	1 cut(s)	968
HinfI	GANTC	5 cut(s)	59, 826, 889, 1019, 1100
HpaII	CCGG	9 cut(s)	48, 159, 165, 179, 201, 222, 455, 1114, 1943
HphI	GGTGA	8 cut(s)	345, 360, 1328, 1543, 1964, 2089, 2219, 2245
Hpy166II	GTNNAC	6 cut(s)	520, 567, 993, 1275, 1540, 2098
Hpy188I	TCNGA	3 cut(s)	449, 1011, 1659
Hpy188III	TCNNGA	7 cut(s)	90, 733, 1381, 1438, 1864, 2143, 2240
Hpy8I	GTNNAC	6 cut(s)	520, 567, 993, 1275, 1540, 2098
Hpy99I	CGWCG	2 cut(s)	32, 35
HpyAV	CCTTC	7 cut(s)	96, 398, 757, 1150, 1154, 1472, 2074
HpyCH4III	ACNGT	5 cut(s)	571, 654, 1747, 1802, 1824
HpyCH4IV	ACGT	4 cut(s)	30, 881, 1185, 2094
HpyCH4V	TGCA	8 cut(s)	40, 347, 410, 520, 582, 802, 1364, 1780
HpyF10VI	GCNNNNNNNGC	9 cut(s)	142, 161, 170, 197, 241, 254, 436, 1056, 1361
HpyF3I	CTNAG	6 cut(s)	146, 885, 1615, 2046, 2185, 2238
HpySE526I	ACGT	4 cut(s)	30, 881, 1185, 2094
Hsp92I	GRCGYC	3 cut(s)	30, 162, 2056
Hsp92II	CATG	4 cut(s)	840, 860, 2011, 2295
HspAI	GCGC	3 cut(s)	162, 458, 2296
KasI	GGCGCC	1 cut(s)	161
KroI	GCCGGC	3 cut(s)	158, 164, 200
KroNI	GCCGGC	3 cut(s)	160, 166, 202
Ksp22I	TGATCA	1 cut(s)	853
Kzo9I	GATC	7 cut(s)	413, 444, 853, 1006, 1023, 1635, 1857
LguI	GCTCTTC	1 cut(s)	2202
LmnI	GCTCC	6 cut(s)	128, 142, 286, 932, 1484, 1938
Lsp1109I	GCAGC	6 cut(s)	29, 94, 947, 1059, 1348, 1376
LweI	GCATC	2 cut(s)	1767, 1945
MabI	ACCWGGT	1 cut(s)	995
MaeI	CTAG	6 cut(s)	213, 671, 833, 893, 1292, 1341
MaeII	ACGT	4 cut(s)	30, 881, 1185, 2094
MaeIII	GTNAC	5 cut(s)	555, 700, 901, 1650, 1730
MalI	GATC	7 cut(s)	415, 446, 855, 1008, 1025, 1637, 1859
MboI	GATC	7 cut(s)	413, 444, 853, 1006, 1023, 1635, 1857
MfeI	CAATTG	1 cut(s)	1989
MhlI	GDGCHC	6 cut(s)	147, 522, 1061, 1167, 1461, 1943
Mly113I	GGCGCC	1 cut(s)	162
MlyI	GAGTC	2 cut(s)	53, 1028
MmeI	TCCRAC	2 cut(s)	150, 603
MreI	CGCCGGCG	1 cut(s)	158
MroNI	GCCGGC	3 cut(s)	158, 164, 200
MroXI	GAANNNNTTC	1 cut(s)	324
MseI	TTAA	9 cut(s)	300, 608, 717, 756, 1014, 1248, 1302, 1571, 2214
MslI	CAYNNNNRTG	3 cut(s)	68, 1035, 2290
MspCI	CTTAAG	1 cut(s)	755
MspI	CCGG	9 cut(s)	48, 159, 165, 179, 201, 222, 455, 1114, 1943
MspR9I	CCNGG	5 cut(s)	222, 250, 284, 997, 1943
MunI	CAATTG	1 cut(s)	1989
Mva1269I	GAATGC	1 cut(s)	349
MvaI	CCWGG	3 cut(s)	250, 284, 997
MvnI	CGCG	1 cut(s)	460
MwoI	GCNNNNNNNGC	9 cut(s)	142, 161, 170, 197, 241, 254, 436, 1056, 1361
NaeI	GCCGGC	3 cut(s)	160, 166, 202
NarI	GGCGCC	1 cut(s)	162
NciI	CCSGG	2 cut(s)	222, 1943
NdeI	CATATG	1 cut(s)	941
NdeII	GATC	7 cut(s)	413, 444, 853, 1006, 1023, 1635, 1857
NgoMIV	GCCGGC	3 cut(s)	158, 164, 200
NlaIII	CATG	4 cut(s)	840, 860, 2011, 2295
NlaIV	GGNNCC	9 cut(s)	116, 124, 163, 281, 288, 1059, 1165, 1799, 1940
NmeAIII	GCCGAG	1 cut(s)	456
NmuCI	GTSAC	1 cut(s)	901
NspV	TTCGAA	1 cut(s)	1199
PaqCI	CACCTGC	1 cut(s)	793
PciSI	GCTCTTC	1 cut(s)	2202
PcsI	WCGNNNNNNNCGW	2 cut(s)	878, 1302
PctI	GAATGC	1 cut(s)	349
PdiI	GCCGGC	3 cut(s)	160, 166, 202
PdmI	GAANNNNTTC	1 cut(s)	324
PfeI	GAWTC	3 cut(s)	826, 889, 1100
PflFI	GACNNNGTC	1 cut(s)	1451
PflMI	CCANNNNNTGG	1 cut(s)	2071
PfoI	TCCNGGA	1 cut(s)	282
PleI	GAGTC	2 cut(s)	53, 1027
PluTI	GGCGCC	1 cut(s)	165
PpsI	GAGTC	2 cut(s)	53, 1027
PshAI	GACNNNNGTC	1 cut(s)	1428
PshBI	ATTAAT	3 cut(s)	300, 717, 1248
Psp124BI	GAGCTC	2 cut(s)	147, 1461
Psp6I	CCWGG	3 cut(s)	248, 282, 995
PspGI	CCWGG	3 cut(s)	248, 282, 995
PspN4I	GGNNCC	9 cut(s)	116, 124, 163, 281, 288, 1059, 1165, 1799, 1940
PspOMI	GGGCCC	2 cut(s)	1057, 1163
PspPI	GGNCC	5 cut(s)	1057, 1058, 1163, 1164, 1798
PstI	CTGCAG	1 cut(s)	1366
PsyI	GACNNNGTC	1 cut(s)	1451
RsaI	GTAC	3 cut(s)	1409, 1821, 1905
RsaNI	GTAC	3 cut(s)	1408, 1820, 1904
RseI	CAYNNNNRTG	3 cut(s)	68, 1035, 2290
SacI	GAGCTC	2 cut(s)	147, 1461
SapI	GCTCTTC	1 cut(s)	2202
SaqAI	TTAA	9 cut(s)	300, 608, 717, 756, 1014, 1248, 1302, 1571, 2214
Sau3AI	GATC	7 cut(s)	413, 444, 853, 1006, 1023, 1635, 1857
Sau96I	GGNCC	5 cut(s)	1057, 1058, 1163, 1164, 1798
ScaI	AGTACT	1 cut(s)	1905
SchI	GAGTC	2 cut(s)	53, 1028
ScrFI	CCNGG	5 cut(s)	222, 250, 284, 997, 1943
SduI	GDGCHC	6 cut(s)	147, 522, 1061, 1167, 1461, 1943
SexAI	ACCWGGT	1 cut(s)	995
SfaNI	GCATC	2 cut(s)	1767, 1945
SfcI	CTRYAG	3 cut(s)	1362, 1590, 2274
SfoI	GGCGCC	1 cut(s)	163
SfuI	TTCGAA	1 cut(s)	1199
SgrAI	CRCCGGYG	2 cut(s)	158, 454
SinI	GGWCC	1 cut(s)	1798
SmiMI	CAYNNNNRTG	3 cut(s)	68, 1035, 2290
SmlI	CTYRAG	3 cut(s)	512, 560, 755
SmoI	CTYRAG	3 cut(s)	512, 560, 755
SpeI	ACTAGT	1 cut(s)	1340
SsiI	CCGC	8 cut(s)	80, 156, 198, 209, 235, 1045, 2075, 2167
SspDI	GGCGCC	1 cut(s)	161
SspI	AATATT	3 cut(s)	637, 721, 2202
SspMI	CTAG	6 cut(s)	213, 671, 833, 893, 1292, 1341
SstI	GAGCTC	2 cut(s)	147, 1461
StyD4I	CCNGG	5 cut(s)	220, 248, 282, 995, 1941
StyI	CCWWGG	1 cut(s)	118
TaaI	ACNGT	5 cut(s)	571, 654, 1747, 1802, 1824
TaiI	ACGT	4 cut(s)	33, 884, 1188, 2097
TaqI	TCGA	7 cut(s)	275, 341, 872, 1022, 1199, 1455, 1495
TatI	WGTACW	3 cut(s)	1407, 1819, 1903
TauI	GCSGC	4 cut(s)	82, 158, 211, 2169
TfiI	GAWTC	3 cut(s)	826, 889, 1100
Tru1I	TTAA	9 cut(s)	300, 608, 717, 756, 1014, 1248, 1302, 1571, 2214
Tru9I	TTAA	9 cut(s)	300, 608, 717, 756, 1014, 1248, 1302, 1571, 2214
TscAI	CASTG	2 cut(s)	1741, 2057
TseFI	GTSAC	1 cut(s)	901
TseI	GCWGC	6 cut(s)	17, 82, 935, 1047, 1361, 1364
Tsp45I	GTSAC	1 cut(s)	901
TspDTI	ATGAA	7 cut(s)	318, 501, 1019, 1220, 1250, 1856, 2024
TspGWI	ACGGA	1 cut(s)	155
TspRI	CASTG	2 cut(s)	1741, 2057
Tth111I	GACNNNGTC	1 cut(s)	1451
Van91I	CCANNNNNTGG	1 cut(s)	2071
Vha464I	CTTAAG	1 cut(s)	755
VneI	GTGCAC	1 cut(s)	518
VpaK11BI	GGWCC	1 cut(s)	1798
VspI	ATTAAT	3 cut(s)	300, 717, 1248
XagI	CCTNNNNNAGG	2 cut(s)	108, 2237
XapI	RAATTY	8 cut(s)	216, 324, 349, 955, 1088, 1218, 1897, 2137
XmiI	GTMKAC	1 cut(s)	1274
XmnI	GAANNNNTTC	1 cut(s)	324
XspI	CTAG	6 cut(s)	213, 671, 833, 893, 1292, 1341
ZraI	GACGTC	1 cut(s)	31
ZrmI	AGTACT	1 cut(s)	1905

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.