Rh2DG089900

Mechanosensitive ion channel protein 10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003680
Physical Location & Seq
Forward (+)
6948009 .. 6952340
4332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG089900.1

Sequence Viewer

Length: 1836 bp
ATGGAAGACAAGAAAGCAGCACCAAACGACGTCGTTGTGCAGATTTCCGGCGCCGAAGACTCCACCTTTATGTTTTCGCCGCAGCCTTCCCGAACTGGGTCTCCTCAAAAAGGGTTCCAAGGCTCCAACTTTGAGCTAACGGAGCTCAGAGTAGCCGCCGGCTCTGCTTCGCCGGAGATCACAGCAACCACCGCCGGAAAGCCGCCGAGAATTCCCGGCGAGCTTCGCCGGGTTTCGCTTGCCAGGTCGGCGTTCTCCAAGCCCAAGTCGAGGTTCCAGGAGCCAACTTACGTTAATGAAGTCAAGGTTGCTGAAGAAATTCCCAAGAGAAAGTCGAGCGCAAATTCACCCAATGTGGCTTCACCAAGCACTAAAGCCGCAGCCACTACTCCCAGAGAAGGTGTAAGATCAAACCCTATAACGCCGAGAACGCCGTTGATCGGAACACCGGCGCGTGGAGAAGAGGATGAGGAAGATGATGAAGAGGTGTACAAGACAGCCAATCTCAAGGTGCACCAGAAATCGGGTAAGAAGCTCAAAAAGTTGGTGTTACTTGAGTGGACTGTGTTTGTGTGCGTTGTTGGGTTTTTAGTTGCTTGCTTAACTGTTGATAAGTTGGAGAGTAAGAATGTTTGGTCATTGGCACTGTGGAAGTGGTGCGTGCTAGTGTTGGTTGTGTTGTGTGGTAGGTTGGTTACAGAATGGCTTATTAACATTTTGGTGTTCGTGATAGAAACGAATTACTTGCTTAAGAAGAAGGTTTTGTATTTTGTTTATGGGTTGAAGAGGAGTGTGCAGGTGTTTATTTGGTTGGGTTTGATTCTACTAGCATGGGGTTTGTTGTTTGATCATGGAGTGAAGAGGTCGAAGAACACGTCTAAGATTCTAGGTTATGTGACAAGGGGTTTGGCTTCTTGTGAGATTGGAGCAGCCATATGGCTGTTGAAGAATTTGTTTGTGAAGCTTTTAGCCACTTCTTTTCAGTCCACCAGGTTCTTTGATAGGATTAAAGAATCAATCTTTCATCAGTATGTTCTCCGCAGCCTATCGGGGCCTCCATTGGTGGAGATGGCTGGAAGAAAATTTGGGAAGGAAACAAGTAGTGGCCGGTTGAGTTTCAAAAATCTGCCAAAAGCTAAAAATGATAGGAAGGAAGGGCCCAAAGAAGAAGTGATTGACGTAGAAAAACTTTCAAAAATGAAGCAGGATAAAATTTCTGCTTGGACGATGAAAGGGTTGATTAATGTGATAAGTGGTTCTGGGTTGTCTACCATCTCCCACACACTAGACGACATTAACGATGAGGAGGTTGAGCAGAGGGATATAGAGATTACTAGTGAGTTTGAAGCACAGGCTGCAGCTTATGACATTTTCCTGAATGTAGCCAAGCGGGGTAGCAAGTACATTGAGGAAGATGACCTCTGTCGCTTCTTGAAAAAGGACGAAGTCGAGCTCGTATTTCCTCTGTTTGAAGGTGGAGCAGAAAGTCGAAAGATAAAAAAAAAGCCTTTGAAGAACTGGCTGGTGAATGTTTACAACGAACGCAAGTCATTGGCACATTCCTTAACTGATACTAAAACAGCTATAGAGGAGTTGAATAAGCTTGGCTCAGCTGTTGTGCTTGTTGTGATCCTTATCGTGTGGTTACTTCTGATGGGATTTTTGACAACCAACATACTGGTCTTTATATCATCACAGCTTTTACTGGTGGTGTTTGTTTTTGGTAACACTGCTAAAACTGTGTTTGAAGCCATCATATTTGTCTTTGTGATGCACCCATTTGATGTAGGGGACCGTTGTGTTGTTGATGGAGTACAGGTAACTCTGAAATCCTAA

Protein Analysis

611

Amino Acids

68.19

Weight (kDa)

9.23

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Msy1 PF25886 187 - 327 7.2e-06 Mechanosensitive ion channel protein Msy1-like, transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 787
AatII GACGTC 1 cut(s) 33
Acc36I ACCTGC 1 cut(s) 787
AccB1I GGYRCC 1 cut(s) 50
AccI GTMKAC 1 cut(s) 1268
AccII CGCG 1 cut(s) 454
AciI CCGC 7 cut(s) 80, 156, 192, 203, 378, 1039, 1390
AclWI GGATC 1 cut(s) 1624
AcoI YGGCCR 1 cut(s) 1105
AcsI RAATTY 6 cut(s) 210, 318, 343, 949, 1082, 1212
AcuI CTGAAG 1 cut(s) 333
AcyI GRCGYC 2 cut(s) 30, 51
AfaI GTAC 3 cut(s) 491, 1403, 1815
AfiI CCNNNNNNNGG 7 cut(s) 96, 110, 270, 398, 440, 455, 523
AflII CTTAAG 1 cut(s) 749
AflIII ACRYGT 1 cut(s) 873
AhlI ACTAGT 1 cut(s) 1334
AjiI CACGTC 1 cut(s) 876
AjnI CCWGG 3 cut(s) 242, 276, 989
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AloI GAACNNNNNNTCC 2 cut(s) 85, 117
Alw21I GWGCWC 3 cut(s) 147, 516, 1455
Alw26I GTCTC 1 cut(s) 105
Alw44I GTGCAC 1 cut(s) 512
AlwI GGATC 1 cut(s) 1624
AoxI GGCC 3 cut(s) 1052, 1105, 1157
ApaI GGGCCC 1 cut(s) 1161
ApaLI GTGCAC 1 cut(s) 512
ApeKI GCWGC 7 cut(s) 17, 82, 380, 929, 1041, 1355, 1358
ApoI RAATTY 6 cut(s) 210, 318, 343, 949, 1082, 1212
ArsI GACNNNNNNTTYG 4 cut(s) 860, 889, 892, 921
AseI ATTAAT 1 cut(s) 1242
Asp700I GAANNNNTTC 1 cut(s) 318
AspLEI GCGC 3 cut(s) 53, 341, 454
AspS9I GGNCC 4 cut(s) 1052, 1157, 1158, 1792
AsuC2I CCSGG 2 cut(s) 216, 230
AsuHPI GGTGA 3 cut(s) 339, 354, 1537
AvaII GGWCC 1 cut(s) 1792
BaeGI GKGCMC 2 cut(s) 516, 1161
BanI GGYRCC 1 cut(s) 50
BanII GRGCYC 3 cut(s) 147, 1161, 1455
BbsI GAAGAC 2 cut(s) 12, 63
Bbv12I GWGCWC 3 cut(s) 147, 516, 1455
BbvI GCAGC 7 cut(s) 29, 94, 392, 941, 1053, 1342, 1370
BccI CCATC 5 cut(s) 1063, 1280, 1648, 1760, 1802
BceAI ACGGC 1 cut(s) 418
BcgI CGANNNNNNTGC 2 cut(s) 727, 761
BciT130I CCWGG 3 cut(s) 244, 278, 991
BclI TGATCA 1 cut(s) 847
BcnI CCSGG 2 cut(s) 216, 230
BcoDI GTCTC 1 cut(s) 105
BcuI ACTAGT 1 cut(s) 1334
BfaI CTAG 5 cut(s) 665, 827, 887, 1286, 1335
BfmI CTRYAG 2 cut(s) 1356, 1584
BfoI RGCGCY 1 cut(s) 54
BfrI CTTAAG 1 cut(s) 749
BfuAI ACCTGC 1 cut(s) 787
BglI GCCNNNNNGGC 1 cut(s) 248
BlpI GCTNAGC 1 cut(s) 1609
Bme1390I CCNGG 5 cut(s) 216, 230, 244, 278, 991
Bme18I GGWCC 1 cut(s) 1792
BmgBI CACGTC 1 cut(s) 876
BmgT120I GGNCC 4 cut(s) 1052, 1157, 1158, 1792
BmiI GGNNCC 8 cut(s) 52, 116, 124, 275, 282, 1053, 1159, 1793
BmrFI CCNGG 5 cut(s) 216, 230, 244, 278, 991
BmrI ACTGGG 1 cut(s) 105
BmsI GCATC 1 cut(s) 1761
BmuI ACTGGG 1 cut(s) 105
BoxI GACNNNNGTC 1 cut(s) 1422
BpiI GAAGAC 2 cut(s) 12, 63
Bpu1102I GCTNAGC 1 cut(s) 1609
BpuEI CTTGAG 2 cut(s) 491, 575
BpuMI CCSGG 2 cut(s) 216, 230
BsaBI GATNNNNATC 1 cut(s) 1634
BsaHI GRCGYC 2 cut(s) 30, 51
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 118
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 1803, 1833
Bsc4I CCNNNNNNNGG 7 cut(s) 96, 110, 270, 398, 440, 455, 523
Bse118I RCCGGY 3 cut(s) 158, 448, 1107
Bse1I ACTGG 4 cut(s) 100, 1523, 1683, 1710
Bse8I GATNNNNATC 1 cut(s) 1634
BseBI CCWGG 3 cut(s) 244, 278, 991
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 1 cut(s) 472
BseJI GATNNNNATC 1 cut(s) 1634
BseLI CCNNNNNNNGG 7 cut(s) 96, 110, 270, 398, 440, 455, 523
BseMII CTCAG 2 cut(s) 160, 1623
BseNI ACTGG 4 cut(s) 100, 1523, 1683, 1710
BseRI GAGGAG 4 cut(s) 93, 802, 1319, 1604
BseSI GKGCMC 2 cut(s) 516, 1161
BseXI GCAGC 7 cut(s) 29, 94, 392, 941, 1053, 1342, 1370
BsgI GTGCAG 2 cut(s) 59, 815
Bsh1236I CGCG 1 cut(s) 454
BshFI GGCC 3 cut(s) 1054, 1107, 1159
BshNI GGYRCC 1 cut(s) 50
BsiHKAI GWGCWC 3 cut(s) 147, 516, 1455
BsiSI CCGG 8 cut(s) 48, 159, 173, 195, 216, 229, 449, 1108
BslFI GGGAC 1 cut(s) 1805
BslI CCNNNNNNNGG 7 cut(s) 96, 110, 270, 398, 440, 455, 523
BsmAI GTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 1805
BsnI GGCC 3 cut(s) 1054, 1107, 1159
Bso31I GGTCTC 1 cut(s) 105
Bsp120I GGGCCC 1 cut(s) 1157
Bsp1286I GDGCHC 4 cut(s) 147, 516, 1161, 1455
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 5 cut(s) 177, 407, 438, 847, 1629
Bsp1720I GCTNAGC 1 cut(s) 1609
BspACI CCGC 7 cut(s) 80, 156, 192, 203, 378, 1039, 1390
BspANI GGCC 3 cut(s) 1054, 1107, 1159
BspCNI CTCAG 2 cut(s) 159, 1622
BspFNI CGCG 1 cut(s) 454
BspLI GGNNCC 8 cut(s) 52, 116, 124, 275, 282, 1053, 1159, 1793
BspMAI CTGCAG 1 cut(s) 1360
BspMI ACCTGC 1 cut(s) 787
BspPI GGATC 1 cut(s) 1624
BspT107I GGYRCC 1 cut(s) 50
BspTI CTTAAG 1 cut(s) 749
BspTNI GGTCTC 1 cut(s) 105
BsrFI RCCGGY 3 cut(s) 158, 448, 1107
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 4 cut(s) 100, 1523, 1683, 1710
BssAI RCCGGY 3 cut(s) 158, 448, 1107
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 5 cut(s) 177, 407, 438, 847, 1629
BssNI GRCGYC 2 cut(s) 30, 51
BssT1I CCWWGG 1 cut(s) 118
Bst2UI CCWGG 3 cut(s) 244, 278, 991
Bst4CI ACNGT 5 cut(s) 565, 607, 648, 1741, 1796
Bst6I CTCTTC 4 cut(s) 456, 477, 779, 854
BstACI GRCGYC 2 cut(s) 30, 51
BstAFI CTTAAG 1 cut(s) 749
BstAPI GCANNNNNTGC 1 cut(s) 1355
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 5 cut(s) 160, 221, 240, 598, 662
BstDEI CTNAG 3 cut(s) 146, 879, 1609
BstENI CCTNNNNNAGG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 472
BstFNI CGCG 1 cut(s) 454
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 3 cut(s) 53, 341, 454
BstKTI GATC 5 cut(s) 180, 410, 441, 850, 1632
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 5 cut(s) 177, 407, 438, 847, 1629
BstMWI GCNNNNNNNGC 7 cut(s) 142, 164, 191, 225, 248, 430, 1355
BstNI CCWGG 3 cut(s) 244, 278, 991
BstPAI GACNNNNGTC 1 cut(s) 1422
BstSCI CCNGG 5 cut(s) 214, 228, 242, 276, 989
BstSFI CTRYAG 2 cut(s) 1356, 1584
BstSLI GKGCMC 2 cut(s) 516, 1161
BstUI CGCG 1 cut(s) 454
BstV1I GCAGC 7 cut(s) 29, 94, 392, 941, 1053, 1342, 1370
BstV2I GAAGAC 2 cut(s) 12, 63
BstXI CCANNNNNNTGG 1 cut(s) 1678
BsuRI GGCC 3 cut(s) 1054, 1107, 1159
BtrI CACGTC 1 cut(s) 876
BtsCI GGATG 1 cut(s) 472
BtsI GCAGTG 1 cut(s) 1728
BtsIMutI CAGTG 2 cut(s) 644, 1728
BveI ACCTGC 1 cut(s) 787
Cac8I GCNNGC 5 cut(s) 160, 221, 240, 598, 662
CfoI GCGC 3 cut(s) 53, 341, 454
Cfr10I RCCGGY 3 cut(s) 158, 448, 1107
Cfr13I GGNCC 4 cut(s) 1052, 1157, 1158, 1792
CsiI ACCWGGT 1 cut(s) 989
Csp6I GTAC 3 cut(s) 490, 1402, 1814
CviAII CATG 2 cut(s) 831, 851
CviQI GTAC 3 cut(s) 490, 1402, 1814
DdeI CTNAG 3 cut(s) 146, 879, 1609
DinI GGCGCC 1 cut(s) 52
DpnI GATC 5 cut(s) 179, 409, 440, 849, 1631
DpnII GATC 5 cut(s) 177, 407, 438, 847, 1629
EaeI YGGCCR 1 cut(s) 1105
Eam1104I CTCTTC 4 cut(s) 456, 477, 779, 854
EarI CTCTTC 4 cut(s) 456, 477, 779, 854
Ecl136II GAGCTC 2 cut(s) 145, 1453
Eco130I CCWWGG 1 cut(s) 118
Eco24I GRGCYC 3 cut(s) 147, 1161, 1455
Eco31I GGTCTC 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 1792
Eco53kI GAGCTC 2 cut(s) 145, 1453
Eco57I CTGAAG 1 cut(s) 333
EcoICRI GAGCTC 2 cut(s) 145, 1453
EcoNI CCTNNNNNAGG 1 cut(s) 108
EcoO109I RGGNCCY 2 cut(s) 1052, 1157
EcoRI GAATTC 1 cut(s) 210
EcoRII CCWGG 3 cut(s) 242, 276, 989
EcoT14I CCWWGG 1 cut(s) 118
EcoT38I GRGCYC 3 cut(s) 147, 1161, 1455
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 2 cut(s) 834, 854
FaqI GGGAC 1 cut(s) 1805
FatI CATG 2 cut(s) 830, 850
FauI CCCGC 1 cut(s) 1383
FauNDI CATATG 1 cut(s) 935
FbaI TGATCA 1 cut(s) 847
FblI GTMKAC 1 cut(s) 1268
FokI GGATG 1 cut(s) 479
FriOI GRGCYC 3 cut(s) 147, 1161, 1455
FspBI CTAG 5 cut(s) 665, 827, 887, 1286, 1335
GlaI GCGC 3 cut(s) 52, 340, 453
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 3 cut(s) 1054, 1107, 1159
HapII CCGG 8 cut(s) 48, 159, 173, 195, 216, 229, 449, 1108
HhaI GCGC 3 cut(s) 53, 341, 454
Hin1I GRCGYC 2 cut(s) 30, 51
Hin1II CATG 2 cut(s) 834, 854
Hin6I GCGC 3 cut(s) 51, 339, 452
HinP1I GCGC 3 cut(s) 51, 339, 452
HindIII AAGCTT 2 cut(s) 962, 1601
HinfI GANTC 4 cut(s) 59, 820, 883, 1013
HpaII CCGG 8 cut(s) 48, 159, 173, 195, 216, 229, 449, 1108
HphI GGTGA 3 cut(s) 339, 354, 1537
Hpy166II GTNNAC 6 cut(s) 490, 514, 561, 987, 1269, 1534
Hpy188I TCNGA 4 cut(s) 149, 443, 1653, 1827
Hpy188III TCNNGA 4 cut(s) 90, 727, 1375, 1432
Hpy8I GTNNAC 6 cut(s) 490, 514, 561, 987, 1269, 1534
Hpy99I CGWCG 2 cut(s) 32, 35
HpyAV CCTTC 7 cut(s) 96, 392, 751, 1084, 1144, 1148, 1466
HpyCH4III ACNGT 5 cut(s) 565, 607, 648, 1741, 1796
HpyCH4IV ACGT 4 cut(s) 30, 291, 875, 1179
HpyCH4V TGCA 5 cut(s) 40, 514, 796, 1358, 1774
HpyF10VI GCNNNNNNNGC 7 cut(s) 142, 164, 191, 225, 248, 430, 1355
HpyF3I CTNAG 3 cut(s) 146, 879, 1609
HpySE526I ACGT 4 cut(s) 30, 291, 875, 1179
Hsp92I GRCGYC 2 cut(s) 30, 51
Hsp92II CATG 2 cut(s) 834, 854
HspAI GCGC 3 cut(s) 51, 339, 452
KasI GGCGCC 1 cut(s) 50
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 5 cut(s) 177, 407, 438, 847, 1629
LmnI GCTCC 5 cut(s) 128, 142, 280, 926, 1478
Lsp1109I GCAGC 7 cut(s) 29, 94, 392, 941, 1053, 1342, 1370
LweI GCATC 1 cut(s) 1761
MabI ACCWGGT 1 cut(s) 989
MaeI CTAG 5 cut(s) 665, 827, 887, 1286, 1335
MaeII ACGT 4 cut(s) 30, 291, 875, 1179
MaeIII GTNAC 6 cut(s) 549, 694, 895, 1644, 1724, 1819
MalI GATC 5 cut(s) 179, 409, 440, 849, 1631
MboI GATC 5 cut(s) 177, 407, 438, 847, 1629
MhlI GDGCHC 4 cut(s) 147, 516, 1161, 1455
MluCI AATT 7 cut(s) 210, 318, 343, 739, 949, 1082, 1212
Mly113I GGCGCC 1 cut(s) 51
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 2 cut(s) 150, 597
MroNI GCCGGC 1 cut(s) 158
MroXI GAANNNNTTC 1 cut(s) 318
MseI TTAA 8 cut(s) 294, 602, 711, 750, 1008, 1242, 1296, 1565
MslI CAYNNNNRTG 3 cut(s) 68, 719, 1029
MspA1I CMGCKG 1 cut(s) 1613
MspCI CTTAAG 1 cut(s) 749
MspI CCGG 8 cut(s) 48, 159, 173, 195, 216, 229, 449, 1108
MspR9I CCNGG 5 cut(s) 216, 230, 244, 278, 991
MvaI CCWGG 3 cut(s) 244, 278, 991
MvnI CGCG 1 cut(s) 454
MwoI GCNNNNNNNGC 7 cut(s) 142, 164, 191, 225, 248, 430, 1355
NaeI GCCGGC 1 cut(s) 160
NarI GGCGCC 1 cut(s) 51
NciI CCSGG 2 cut(s) 216, 230
NdeI CATATG 1 cut(s) 935
NdeII GATC 5 cut(s) 177, 407, 438, 847, 1629
NgoMIV GCCGGC 1 cut(s) 158
NlaIII CATG 2 cut(s) 834, 854
NlaIV GGNNCC 8 cut(s) 52, 116, 124, 275, 282, 1053, 1159, 1793
NmeAIII GCCGAG 2 cut(s) 231, 450
NmuCI GTSAC 1 cut(s) 895
PaqCI CACCTGC 1 cut(s) 787
PcsI WCGNNNNNNNCGW 2 cut(s) 872, 1296
PdiI GCCGGC 1 cut(s) 160
PdmI GAANNNNTTC 1 cut(s) 318
PfeI GAWTC 3 cut(s) 820, 883, 1013
PflFI GACNNNGTC 1 cut(s) 1445
PfoI TCCNGGA 1 cut(s) 276
PleI GAGTC 1 cut(s) 53
PluTI GGCGCC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 53
PshAI GACNNNNGTC 1 cut(s) 1422
PshBI ATTAAT 1 cut(s) 1242
Psp124BI GAGCTC 2 cut(s) 147, 1455
Psp6I CCWGG 3 cut(s) 242, 276, 989
PspGI CCWGG 3 cut(s) 242, 276, 989
PspN4I GGNNCC 8 cut(s) 52, 116, 124, 275, 282, 1053, 1159, 1793
PspOMI GGGCCC 1 cut(s) 1157
PspPI GGNCC 4 cut(s) 1052, 1157, 1158, 1792
PstI CTGCAG 1 cut(s) 1360
PsyI GACNNNGTC 1 cut(s) 1445
PvuII CAGCTG 1 cut(s) 1613
RsaI GTAC 3 cut(s) 491, 1403, 1815
RsaNI GTAC 3 cut(s) 490, 1402, 1814
RseI CAYNNNNRTG 3 cut(s) 68, 719, 1029
SacI GAGCTC 2 cut(s) 147, 1455
SaqAI TTAA 8 cut(s) 294, 602, 711, 750, 1008, 1242, 1296, 1565
Sau3AI GATC 5 cut(s) 177, 407, 438, 847, 1629
Sau96I GGNCC 4 cut(s) 1052, 1157, 1158, 1792
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 5 cut(s) 216, 230, 244, 278, 991
SduI GDGCHC 4 cut(s) 147, 516, 1161, 1455
SexAI ACCWGGT 1 cut(s) 989
SfaNI GCATC 1 cut(s) 1761
SfcI CTRYAG 2 cut(s) 1356, 1584
SfoI GGCGCC 1 cut(s) 52
SgrAI CRCCGGYG 1 cut(s) 448
SinI GGWCC 1 cut(s) 1792
SmiMI CAYNNNNRTG 3 cut(s) 68, 719, 1029
SmlI CTYRAG 3 cut(s) 506, 554, 749
SmoI CTYRAG 3 cut(s) 506, 554, 749
SpeI ACTAGT 1 cut(s) 1334
Sse9I AATT 7 cut(s) 210, 318, 343, 739, 949, 1082, 1212
SsiI CCGC 7 cut(s) 80, 156, 192, 203, 378, 1039, 1390
SspDI GGCGCC 1 cut(s) 50
SspMI CTAG 5 cut(s) 665, 827, 887, 1286, 1335
SstI GAGCTC 2 cut(s) 147, 1455
StyD4I CCNGG 5 cut(s) 214, 228, 242, 276, 989
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 5 cut(s) 565, 607, 648, 1741, 1796
TaiI ACGT 4 cut(s) 33, 294, 878, 1182
TaqI TCGA 5 cut(s) 269, 335, 866, 1449, 1489
TasI AATT 7 cut(s) 210, 318, 343, 739, 949, 1082, 1212
TatI WGTACW 3 cut(s) 489, 1401, 1813
TauI GCSGC 4 cut(s) 82, 158, 205, 380
TfiI GAWTC 3 cut(s) 820, 883, 1013
Tru1I TTAA 8 cut(s) 294, 602, 711, 750, 1008, 1242, 1296, 1565
Tru9I TTAA 8 cut(s) 294, 602, 711, 750, 1008, 1242, 1296, 1565
TscAI CASTG 2 cut(s) 651, 1735
TseFI GTSAC 1 cut(s) 895
TseI GCWGC 7 cut(s) 17, 82, 380, 929, 1041, 1355, 1358
Tsp45I GTSAC 1 cut(s) 895
TspDTI ATGAA 5 cut(s) 312, 495, 1013, 1214, 1244
TspGWI ACGGA 1 cut(s) 155
TspRI CASTG 2 cut(s) 651, 1735
Tth111I GACNNNGTC 1 cut(s) 1445
Vha464I CTTAAG 1 cut(s) 749
VneI GTGCAC 1 cut(s) 512
VpaK11BI GGWCC 1 cut(s) 1792
VspI ATTAAT 1 cut(s) 1242
XagI CCTNNNNNAGG 1 cut(s) 108
XapI RAATTY 6 cut(s) 210, 318, 343, 949, 1082, 1212
XmiI GTMKAC 1 cut(s) 1268
XmnI GAANNNNTTC 1 cut(s) 318
XspI CTAG 5 cut(s) 665, 827, 887, 1286, 1335
ZraI GACGTC 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.