FvH4_1g12390

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005515
Physical Location & Seq
Reverse (-)
6788805 .. 6790958
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g12390.t1

Sequence Viewer

Length: 744 bp
ATGGCTGCACTATTCAGAATTGAACAGGTTCACTTAAATAGAAAAGACGGCAAAGCCAAGGTGCTTGGAACTCTGGCTTCTGTTGCTGGAGCCTTAGTCATAACCCTTTACAAAGGGCCAACAATTTATGGACCTCCAAGTGATTCACAATATTTAAACCAGTCACATTCTCGGTTATCATTACTTGGAGATGCCAAGGAGAAGAACTGGACTTTGGGATGCATATGTTTAATTGGTCATTGTCTGTGTTGGTCTAGCTGGATTGTGCTACAAGTACCTGTTTTGAAGAACTACCCAGCTCGCCTCTCTGTCTCCTCTTTTACTTGCTTCTTTGGCATTCTGCAGTGTTCTGCAATCGCGGGGATTGTAGAAAGAGAATCACAAGCTTGGCAGATCCATTCTGGTGGTGAAGTTTTCGCTATTCTCTACGCGGGAGTGGTGGCTTCAGCAATGGCGTTCGCAATACAGATATGGGTGATCGAAAAAGGAGGACCAGTGTTCGTCTCAGTGTACCTGCCTCTACAGACTTTACTCGTAGCTCTAATGGCCTCGGTCATTTTGGGTGAACAATTCTACATGGGAGGCATTATTGGAGCGGTATTGATTGTAGCTGGACTATACCTGGTGGTCTGGGGGAAAAATGAAGAGAGCAAGTTTGGCAAGGAAAGGGGTGAAATTCCCTCAATGCCTGCAGATGAAGGATCCTTTCTGTTTCAGTCATTACTCGCTTCCTCTTCCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

248

Amino Acids

26.65

Weight (kDa)

8.18

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 72 - 210 1.7e-15 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 522
AccBSI CCGCTC 1 cut(s) 596
AccII CGCG 2 cut(s) 359, 431
AciI CCGC 3 cut(s) 359, 431, 596
AclWI GGATC 3 cut(s) 388, 696, 709
AcsI RAATTY 1 cut(s) 675
AcuI CTGAAG 1 cut(s) 429
AfaI GTAC 2 cut(s) 276, 512
AgsI TTSAA 2 cut(s) 23, 286
AjnI CCWGG 1 cut(s) 621
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 5 cut(s) 258, 299, 386, 539, 611
AluI AGCT 5 cut(s) 258, 299, 386, 539, 611
Alw26I GTCTC 2 cut(s) 316, 508
AlwI GGATC 3 cut(s) 388, 696, 709
AoxI GGCC 2 cut(s) 116, 546
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 675
Asp700I GAANNNNTTC 1 cut(s) 27
AspS9I GGNCC 3 cut(s) 116, 131, 491
AsuHPI GGTGA 4 cut(s) 419, 487, 575, 683
AvaII GGWCC 2 cut(s) 131, 491
BamHI GGATCC 1 cut(s) 701
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 623
BcoDI GTCTC 2 cut(s) 316, 508
BfaI CTAG 1 cut(s) 255
BfmI CTRYAG 3 cut(s) 341, 521, 690
BfuAI ACCTGC 1 cut(s) 522
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 623
Bme18I GGWCC 2 cut(s) 131, 491
BmgT120I GGNCC 3 cut(s) 116, 131, 491
BmiI GGNNCC 2 cut(s) 91, 703
BmrFI CCNGG 1 cut(s) 623
BmsI GCATC 2 cut(s) 181, 209
BpmI CTGGAG 1 cut(s) 108
BsaJI CCNNGG 3 cut(s) 57, 195, 549
BsaXI ACNNNNNCTCC 4 cut(s) 81, 111, 480, 510
Bse1I ACTGG 3 cut(s) 160, 212, 494
Bse3DI GCAATG 1 cut(s) 456
BseBI CCWGG 1 cut(s) 623
BseDI CCNNGG 3 cut(s) 57, 195, 549
BseGI GGATG 1 cut(s) 224
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 1 cut(s) 519
BseNI ACTGG 3 cut(s) 160, 212, 494
BseRI GAGGAG 1 cut(s) 304
BseYI CCCAGC 1 cut(s) 295
Bsh1236I CGCG 2 cut(s) 359, 431
BshFI GGCC 2 cut(s) 118, 548
BsmAI GTCTC 2 cut(s) 316, 508
BsmBI CGTCTC 1 cut(s) 508
BsmI GAATGC 1 cut(s) 336
BsnI GGCC 2 cut(s) 118, 548
Bsp143I GATC 3 cut(s) 393, 477, 701
BspACI CCGC 3 cut(s) 359, 431, 596
BspANI GGCC 2 cut(s) 118, 548
BspCNI CTCAG 1 cut(s) 518
BspFNI CGCG 2 cut(s) 359, 431
BspLI GGNNCC 2 cut(s) 91, 703
BspMAI CTGCAG 2 cut(s) 345, 694
BspMI ACCTGC 1 cut(s) 522
BspPI GGATC 3 cut(s) 388, 696, 709
BsrBI CCGCTC 1 cut(s) 596
BsrDI GCAATG 1 cut(s) 456
BsrI ACTGG 3 cut(s) 160, 212, 494
BssECI CCNNGG 3 cut(s) 57, 195, 549
BssMI GATC 3 cut(s) 393, 477, 701
BssT1I CCWWGG 2 cut(s) 57, 195
Bst2UI CCWGG 1 cut(s) 623
Bst6I CTCTTC 2 cut(s) 639, 739
BstC8I GCNNGC 2 cut(s) 301, 690
BstDEI CTNAG 2 cut(s) 94, 505
BstF5I GGATG 1 cut(s) 224
BstFNI CGCG 2 cut(s) 359, 431
BstKTI GATC 3 cut(s) 396, 480, 704
BstMAI GTCTC 2 cut(s) 316, 508
BstMBI GATC 3 cut(s) 393, 477, 701
BstMWI GCNNNNNNNGC 4 cut(s) 83, 333, 545, 657
BstNI CCWGG 1 cut(s) 623
BstSCI CCNGG 1 cut(s) 621
BstSFI CTRYAG 3 cut(s) 341, 521, 690
BstUI CGCG 2 cut(s) 359, 431
BstX2I RGATCY 2 cut(s) 393, 701
BstXI CCANNNNNNTGG 1 cut(s) 404
BstYI RGATCY 2 cut(s) 393, 701
BsuRI GGCC 2 cut(s) 118, 548
BtsCI GGATG 1 cut(s) 224
BtsI GCAGTG 1 cut(s) 350
BtsIMutI CAGTG 3 cut(s) 350, 501, 513
BveI ACCTGC 1 cut(s) 522
Cac8I GCNNGC 2 cut(s) 301, 690
Cfr13I GGNCC 3 cut(s) 116, 131, 491
CsiI ACCWGGT 1 cut(s) 621
Csp6I GTAC 2 cut(s) 275, 511
CviAII CATG 1 cut(s) 577
CviQI GTAC 2 cut(s) 275, 511
DdeI CTNAG 2 cut(s) 94, 505
DpnI GATC 3 cut(s) 395, 479, 703
DpnII GATC 3 cut(s) 393, 477, 701
DraI TTTAAA 1 cut(s) 156
Eam1104I CTCTTC 2 cut(s) 639, 739
EarI CTCTTC 2 cut(s) 639, 739
Eco130I CCWWGG 2 cut(s) 57, 195
Eco47I GGWCC 2 cut(s) 131, 491
Eco57I CTGAAG 1 cut(s) 429
EcoRII CCWGG 1 cut(s) 621
EcoT14I CCWWGG 2 cut(s) 57, 195
EcoT22I ATGCAT 1 cut(s) 224
ErhI CCWWGG 2 cut(s) 57, 195
Esp3I CGTCTC 1 cut(s) 508
FaeI CATG 1 cut(s) 580
FaiI YATR 7 cut(s) 101, 129, 224, 226, 472, 578, 619
FatI CATG 1 cut(s) 576
FauI CCCGC 2 cut(s) 352, 424
FauNDI CATATG 1 cut(s) 224
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 1 cut(s) 231
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 255
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 1 cut(s) 299
GsuI CTGGAG 1 cut(s) 108
HaeIII GGCC 2 cut(s) 118, 548
Hin1II CATG 1 cut(s) 580
HindIII AAGCTT 1 cut(s) 384
HinfI GANTC 2 cut(s) 143, 377
HphI GGTGA 4 cut(s) 419, 487, 575, 683
Hpy166II GTNNAC 3 cut(s) 31, 511, 566
Hpy188I TCNGA 1 cut(s) 17
Hpy8I GTNNAC 3 cut(s) 31, 511, 566
HpyAV CCTTC 1 cut(s) 692
HpyCH4V TGCA 5 cut(s) 8, 222, 343, 353, 692
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 333, 545, 657
HpyF3I CTNAG 2 cut(s) 94, 505
Hsp92II CATG 1 cut(s) 580
Kzo9I GATC 3 cut(s) 393, 477, 701
LmnI GCTCC 2 cut(s) 89, 593
LweI GCATC 2 cut(s) 181, 209
MabI ACCWGGT 1 cut(s) 621
MaeI CTAG 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 162
MalI GATC 3 cut(s) 395, 479, 703
MbiI CCGCTC 1 cut(s) 596
MboI GATC 3 cut(s) 393, 477, 701
MboII GAAGA 4 cut(s) 214, 298, 656, 726
MflI RGATCY 2 cut(s) 393, 701
MluCI AATT 5 cut(s) 18, 123, 231, 569, 675
MnlI CCTC 9 cut(s) 144, 314, 325, 482, 528, 559, 575, 691, 742
Mph1103I ATGCAT 1 cut(s) 224
MroXI GAANNNNTTC 1 cut(s) 27
MseI TTAA 3 cut(s) 35, 155, 230
MslI CAYNNNNRTG 1 cut(s) 402
MspR9I CCNGG 1 cut(s) 623
Mva1269I GAATGC 1 cut(s) 336
MvaI CCWGG 1 cut(s) 623
MvnI CGCG 2 cut(s) 359, 431
MwoI GCNNNNNNNGC 4 cut(s) 83, 333, 545, 657
NdeI CATATG 1 cut(s) 224
NdeII GATC 3 cut(s) 393, 477, 701
NlaIII CATG 1 cut(s) 580
NlaIV GGNNCC 2 cut(s) 91, 703
NmuCI GTSAC 1 cut(s) 162
NsiI ATGCAT 1 cut(s) 224
PctI GAATGC 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 27
PfeI GAWTC 2 cut(s) 143, 377
PkrI GCNGC 1 cut(s) 7
Psp6I CCWGG 1 cut(s) 621
PspFI CCCAGC 1 cut(s) 295
PspGI CCWGG 1 cut(s) 621
PspN4I GGNNCC 2 cut(s) 91, 703
PspPI GGNCC 3 cut(s) 116, 131, 491
PstI CTGCAG 2 cut(s) 345, 694
PsuI RGATCY 2 cut(s) 393, 701
RsaI GTAC 2 cut(s) 276, 512
RsaNI GTAC 2 cut(s) 275, 511
RseI CAYNNNNRTG 1 cut(s) 402
SaqAI TTAA 3 cut(s) 35, 155, 230
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 3 cut(s) 393, 477, 701
Sau96I GGNCC 3 cut(s) 116, 131, 491
ScrFI CCNGG 1 cut(s) 623
SexAI ACCWGGT 1 cut(s) 621
SfaNI GCATC 2 cut(s) 181, 209
SfcI CTRYAG 3 cut(s) 341, 521, 690
SinI GGWCC 2 cut(s) 131, 491
SmiMI CAYNNNNRTG 1 cut(s) 402
Sse9I AATT 5 cut(s) 18, 123, 231, 569, 675
SsiI CCGC 3 cut(s) 359, 431, 596
SspI AATATT 1 cut(s) 152
SspMI CTAG 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 621
StyI CCWWGG 2 cut(s) 57, 195
TaqI TCGA 1 cut(s) 480
TaqII GACCGA 1 cut(s) 541
TasI AATT 5 cut(s) 18, 123, 231, 569, 675
TfiI GAWTC 2 cut(s) 143, 377
Tru1I TTAA 3 cut(s) 35, 155, 230
Tru9I TTAA 3 cut(s) 35, 155, 230
TscAI CASTG 3 cut(s) 350, 501, 513
TseFI GTSAC 1 cut(s) 162
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 162
TspDTI ATGAA 2 cut(s) 657, 711
TspRI CASTG 3 cut(s) 350, 501, 513
VpaK11BI GGWCC 2 cut(s) 131, 491
XapI RAATTY 1 cut(s) 675
XmnI GAANNNNTTC 1 cut(s) 27
XspI CTAG 1 cut(s) 255
Zsp2I ATGCAT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.