RLG00000016948

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005515
Physical Location & Seq
Reverse (-)
11486881 .. 11488728
1848 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016948

Sequence Viewer

Length: 1161 bp
ATGGCTGATGAGGCCGGTTCACATGGCAAGAGAGAGTGTATGGCGCCTGAACGTGCGAGGATTCACATGGCTTTAACTTTCCTGCAGTCGAGCAATGCAGTGCAACATATCGTCATCAGGAGTGTGCTTAACATGGGCGTAAACCAGCTTATTTTCCCAGTATACAGGAGCATCACCGCGCTGCTTCTGCTTTGTCCCTATGCATACTTTTTGGAGAGAAAAGATAGACCACCACTGAATATATCCCTTGTTATGCAGTTTTTTCTCCTGGGACTTATTGGGGTCACCTGCAATCAAGGATTCTACCTCTTGGGTTTGGAGAACACATCAGCCACATTTGTTTCTGCAGTAGAGAATGCAGTCCCTGCAGGAACATTTCTCATGGCTGCCGTATTAAGAATTGAACTAGTTCACTTGAATAGAAAAGATGGCAAAGCCCAAGTGCTTGGAGTTCTGGCTTCTGTTGCAGGAGCCTCAGTCATAACCTTTTACAAGGGGCCAACTGTATTTGTTGCTCCAAGGACTTCACCTTCAGGCCAATCCCATATGCTCCTCTCGTTATTTGGAGATGCCAGGGTGAAGAACAGGACCTTTGGTTGCATTTGTGCTATAGGTCATTGCGTATGTTGGTTTAGCTGGATTGTCCTACAAGGACCTGTTTTGAAGAAGTACCCAGCTCGCCTCTCAGTCACCTCATTTACTCTCTTCTTTGGCATTCTGCAACTTTCAGCAATTGCAGCGTATGTAGAAAGAGACTCCCAAGAGTGGCAGATCCATTCCGGCAGTGCAGTCTTGGGCATTCTCTACGTGGGAGTGGTGGCTTCAGCAATGTCTTATGCAATACAGCTATGGGCGATTGACAAGGGAGGACCAGTGTTTGTTTCAGTGTATATGCCTCTACAGACGTTACTCGTAGATATAATGGACTCGGTCATTATGGGTGCACAATTCTACTTGGGAGGCATTGTTGGAGCAGTGCTGATCGTAGCAGGACTATACCTTGTAGTGTGGGGAAAAAGCAAAGAGAGCAAATTTGGCAAAGAAATGAAGTCGAAAAGTTCGCCAGTGCCACTGAACATTGATAATCAGATTAAATATTATTCTGAAGAAGAAAAAGAATCCTCTCTCTTCCAACCATTGCTCCCTACCTCTTCAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

387

Amino Acids

42.16

Weight (kDa)

9.06

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 37 - 162 5.6e-11 EamA-like transporter family
EamA PF00892 198 - 336 2.5e-13 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 296
Acc36I ACCTGC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 162
AccII CGCG 1 cut(s) 179
AciI CCGC 1 cut(s) 177
AclWI GGATC 1 cut(s) 766
AcsI RAATTY 1 cut(s) 1031
AcuI CTGAAG 3 cut(s) 516, 807, 1125
AcyI GRCGYC 1 cut(s) 44
AfaI GTAC 1 cut(s) 671
AfiI CCNNNNNNNGG 1 cut(s) 765
AgsI TTSAA 4 cut(s) 404, 418, 664, 1155
AhlI ACTAGT 1 cut(s) 406
AjnI CCWGG 2 cut(s) 267, 572
AluBI AGCT 4 cut(s) 148, 636, 677, 847
AluI AGCT 4 cut(s) 148, 636, 677, 847
Alw21I GWGCWC 1 cut(s) 946
Alw26I GTCTC 1 cut(s) 747
Alw44I GTGCAC 1 cut(s) 942
AlwI GGATC 1 cut(s) 766
AlwNI CAGNNNCTG 1 cut(s) 365
AoxI GGCC 3 cut(s) 12, 497, 535
ApaLI GTGCAC 1 cut(s) 942
ApeKI GCWGC 3 cut(s) 181, 386, 737
ApoI RAATTY 1 cut(s) 1031
Asp700I GAANNNNTTC 1 cut(s) 408
AspLEI GCGC 2 cut(s) 46, 181
AspS9I GGNCC 4 cut(s) 497, 588, 653, 869
AsuHPI GGTGA 5 cut(s) 166, 277, 519, 589, 682
AvaII GGWCC 3 cut(s) 588, 653, 869
BaeGI GKGCMC 1 cut(s) 946
BanI GGYRCC 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 946
BbvI GCAGC 3 cut(s) 168, 373, 749
BccI CCATC 1 cut(s) 422
BceAI ACGGC 1 cut(s) 374
BciT130I CCWGG 2 cut(s) 269, 574
BcoDI GTCTC 1 cut(s) 747
BcuI ACTAGT 1 cut(s) 406
BfaI CTAG 1 cut(s) 407
BfmI CTRYAG 5 cut(s) 83, 345, 366, 609, 899
BfoI RGCGCY 1 cut(s) 47
BfuAI ACCTGC 1 cut(s) 296
BisI GCNGC 3 cut(s) 182, 387, 738
BlsI GCNGC 3 cut(s) 183, 388, 739
Bme1390I CCNGG 2 cut(s) 269, 574
Bme18I GGWCC 3 cut(s) 588, 653, 869
BmgT120I GGNCC 4 cut(s) 497, 588, 653, 869
BmiI GGNNCC 3 cut(s) 45, 472, 498
BmrFI CCNGG 2 cut(s) 269, 574
BmrI ACTGGG 1 cut(s) 152
BmsI GCATC 2 cut(s) 180, 559
BmuI ACTGGG 1 cut(s) 152
BsaAI YACGTR 1 cut(s) 808
BsaHI GRCGYC 1 cut(s) 44
BsaJI CCNNGG 3 cut(s) 268, 518, 573
BsaXI ACNNNNNCTCC 8 cut(s) 462, 492, 858, 888, 951, 981, 1125, 1155
Bsc4I CCNNNNNNNGG 1 cut(s) 765
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 3 cut(s) 158, 872, 1064
Bse3DI GCAATG 4 cut(s) 100, 616, 834, 1136
BseBI CCWGG 2 cut(s) 269, 574
BseDI CCNNGG 3 cut(s) 268, 518, 573
BseLI CCNNNNNNNGG 1 cut(s) 765
BseMI GCAATG 4 cut(s) 100, 616, 834, 1136
BseMII CTCAG 2 cut(s) 489, 699
BseNI ACTGG 3 cut(s) 158, 872, 1064
BseRI GAGGAG 1 cut(s) 542
BseSI GKGCMC 1 cut(s) 946
BseXI GCAGC 3 cut(s) 168, 373, 749
BseYI CCCAGC 1 cut(s) 673
BsgI GTGCAG 1 cut(s) 807
Bsh1236I CGCG 1 cut(s) 179
BshFI GGCC 3 cut(s) 14, 499, 537
BshNI GGYRCC 1 cut(s) 43
BsiHKAI GWGCWC 1 cut(s) 946
BsiSI CCGG 2 cut(s) 15, 780
BslFI GGGAC 3 cut(s) 180, 285, 347
BslI CCNNNNNNNGG 1 cut(s) 765
BsmAI GTCTC 1 cut(s) 747
BsmFI GGGAC 3 cut(s) 180, 285, 347
BsmI GAATGC 3 cut(s) 361, 714, 798
BsnI GGCC 3 cut(s) 14, 499, 537
Bsp1286I GDGCHC 1 cut(s) 946
Bsp143I GATC 2 cut(s) 771, 981
BspACI CCGC 1 cut(s) 177
BspANI GGCC 3 cut(s) 14, 499, 537
BspCNI CTCAG 2 cut(s) 488, 698
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 3 cut(s) 45, 472, 498
BspMAI CTGCAG 3 cut(s) 87, 349, 370
BspMI ACCTGC 1 cut(s) 296
BspPI GGATC 1 cut(s) 766
BspT107I GGYRCC 1 cut(s) 43
BsrDI GCAATG 4 cut(s) 100, 616, 834, 1136
BsrFI RCCGGY 1 cut(s) 14
BsrI ACTGG 3 cut(s) 158, 872, 1064
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 3 cut(s) 268, 518, 573
BssMI GATC 2 cut(s) 771, 981
BssNAI GTATAC 1 cut(s) 163
BssNI GRCGYC 1 cut(s) 44
BssT1I CCWWGG 1 cut(s) 518
Bst1107I GTATAC 1 cut(s) 163
Bst2UI CCWGG 2 cut(s) 269, 574
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 3 cut(s) 710, 1133, 1156
BstACI GRCGYC 1 cut(s) 44
BstAPI GCANNNNNTGC 1 cut(s) 365
BstBAI YACGTR 1 cut(s) 808
BstC8I GCNNGC 1 cut(s) 679
BstDEI CTNAG 2 cut(s) 475, 685
BstEII GGTNACC 1 cut(s) 283
BstFNI CGCG 1 cut(s) 179
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 2 cut(s) 46, 181
BstKTI GATC 2 cut(s) 774, 984
BstMAI GTCTC 1 cut(s) 747
BstMBI GATC 2 cut(s) 771, 981
BstMWI GCNNNNNNNGC 7 cut(s) 11, 187, 365, 464, 737, 1026, 1035
BstNI CCWGG 2 cut(s) 269, 574
BstPI GGTNACC 1 cut(s) 283
BstSCI CCNGG 2 cut(s) 267, 572
BstSFI CTRYAG 5 cut(s) 83, 345, 366, 609, 899
BstSLI GKGCMC 1 cut(s) 946
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 3 cut(s) 168, 373, 749
BstX2I RGATCY 1 cut(s) 771
BstXI CCANNNNNNTGG 1 cut(s) 446
BstYI RGATCY 1 cut(s) 771
BstZ17I GTATAC 1 cut(s) 163
BsuRI GGCC 3 cut(s) 14, 499, 537
BtsI GCAGTG 3 cut(s) 105, 790, 981
BtsIMutI CAGTG 8 cut(s) 105, 233, 790, 879, 891, 981, 1070, 1071
BveI ACCTGC 1 cut(s) 296
Cac8I GCNNGC 1 cut(s) 679
CaiI CAGNNNCTG 1 cut(s) 365
CfoI GCGC 2 cut(s) 46, 181
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 4 cut(s) 497, 588, 653, 869
Csp6I GTAC 1 cut(s) 670
CviAII CATG 4 cut(s) 23, 67, 133, 382
CviQI GTAC 1 cut(s) 670
DdeI CTNAG 2 cut(s) 475, 685
DinI GGCGCC 1 cut(s) 45
DpnI GATC 2 cut(s) 773, 983
DpnII GATC 2 cut(s) 771, 981
Eam1104I CTCTTC 3 cut(s) 710, 1133, 1156
EarI CTCTTC 3 cut(s) 710, 1133, 1156
Eco130I CCWWGG 1 cut(s) 518
Eco47I GGWCC 3 cut(s) 588, 653, 869
Eco57I CTGAAG 3 cut(s) 516, 807, 1125
Eco91I GGTNACC 1 cut(s) 283
EcoO109I RGGNCCY 2 cut(s) 588, 653
EcoO65I GGTNACC 1 cut(s) 283
EcoRII CCWGG 2 cut(s) 267, 572
EcoT14I CCWWGG 1 cut(s) 518
EcoT22I ATGCAT 1 cut(s) 205
EgeI GGCGCC 1 cut(s) 45
EheI GGCGCC 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 518
FaeI CATG 4 cut(s) 26, 70, 136, 385
FaqI GGGAC 3 cut(s) 180, 285, 347
FatI CATG 4 cut(s) 22, 66, 132, 381
FauNDI CATATG 1 cut(s) 546
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 3 cut(s) 182, 387, 738
Fsp4HI GCNGC 3 cut(s) 182, 387, 738
FspBI CTAG 1 cut(s) 407
GlaI GCGC 2 cut(s) 45, 180
GluI GCNGC 3 cut(s) 182, 387, 738
GsaI CCCAGC 1 cut(s) 677
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 3 cut(s) 14, 499, 537
HapII CCGG 2 cut(s) 15, 780
HhaI GCGC 2 cut(s) 46, 181
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 4 cut(s) 26, 70, 136, 385
Hin6I GCGC 2 cut(s) 44, 179
HinP1I GCGC 2 cut(s) 44, 179
HinfI GANTC 5 cut(s) 61, 300, 755, 926, 1118
HpaII CCGG 2 cut(s) 15, 780
HphI GGTGA 5 cut(s) 166, 277, 519, 589, 682
Hpy166II GTNNAC 5 cut(s) 20, 142, 163, 412, 944
Hpy188I TCNGA 2 cut(s) 1089, 1105
Hpy188III TCNNGA 1 cut(s) 118
Hpy8I GTNNAC 5 cut(s) 20, 142, 163, 412, 944
HpyAV CCTTC 1 cut(s) 540
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4IV ACGT 3 cut(s) 52, 807, 905
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 187, 365, 464, 737, 1026, 1035
HpyF3I CTNAG 2 cut(s) 475, 685
HpySE526I ACGT 3 cut(s) 52, 807, 905
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 4 cut(s) 26, 70, 136, 385
HspAI GCGC 2 cut(s) 44, 179
KasI GGCGCC 1 cut(s) 43
Kzo9I GATC 2 cut(s) 771, 981
LmnI GCTCC 6 cut(s) 168, 470, 520, 555, 971, 1146
Lsp1109I GCAGC 3 cut(s) 168, 373, 749
LweI GCATC 2 cut(s) 180, 559
MaeI CTAG 1 cut(s) 407
MaeII ACGT 3 cut(s) 52, 807, 905
MaeIII GTNAC 3 cut(s) 283, 688, 906
MalI GATC 2 cut(s) 773, 983
MboI GATC 2 cut(s) 771, 981
MboII GAAGA 7 cut(s) 592, 676, 697, 1118, 1120, 1121, 1143
MfeI CAATTG 1 cut(s) 732
MflI RGATCY 1 cut(s) 771
MhlI GDGCHC 1 cut(s) 946
MluCI AATT 4 cut(s) 399, 732, 947, 1031
Mly113I GGCGCC 1 cut(s) 44
MlyI GAGTC 2 cut(s) 749, 920
MmeI TCCRAC 2 cut(s) 949, 1156
Mph1103I ATGCAT 1 cut(s) 205
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 4 cut(s) 74, 129, 395, 1092
MspI CCGG 2 cut(s) 15, 780
MspR9I CCNGG 2 cut(s) 269, 574
MunI CAATTG 1 cut(s) 732
Mva1269I GAATGC 3 cut(s) 361, 714, 798
MvaI CCWGG 2 cut(s) 269, 574
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 7 cut(s) 11, 187, 365, 464, 737, 1026, 1035
NarI GGCGCC 1 cut(s) 44
NdeI CATATG 1 cut(s) 546
NdeII GATC 2 cut(s) 771, 981
NlaIII CATG 4 cut(s) 26, 70, 136, 385
NlaIV GGNNCC 3 cut(s) 45, 472, 498
NmuCI GTSAC 2 cut(s) 283, 688
NsiI ATGCAT 1 cut(s) 205
PaqCI CACCTGC 1 cut(s) 296
PctI GAATGC 3 cut(s) 361, 714, 798
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 3 cut(s) 61, 300, 1118
PflFI GACNNNGTC 1 cut(s) 929
PkrI GCNGC 3 cut(s) 183, 388, 739
PleI GAGTC 2 cut(s) 749, 920
PluTI GGCGCC 1 cut(s) 47
PpsI GAGTC 2 cut(s) 749, 920
Ppu21I YACGTR 1 cut(s) 808
PpuMI RGGWCCY 2 cut(s) 588, 653
Psp5II RGGWCCY 2 cut(s) 588, 653
Psp6I CCWGG 2 cut(s) 267, 572
PspEI GGTNACC 1 cut(s) 283
PspFI CCCAGC 1 cut(s) 673
PspGI CCWGG 2 cut(s) 267, 572
PspN4I GGNNCC 3 cut(s) 45, 472, 498
PspPI GGNCC 4 cut(s) 497, 588, 653, 869
PspPPI RGGWCCY 2 cut(s) 588, 653
PstI CTGCAG 3 cut(s) 87, 349, 370
PstNI CAGNNNCTG 1 cut(s) 365
PsuI RGATCY 1 cut(s) 771
PsyI GACNNNGTC 1 cut(s) 929
RsaI GTAC 1 cut(s) 671
RsaNI GTAC 1 cut(s) 670
SaqAI TTAA 4 cut(s) 74, 129, 395, 1092
SatI GCNGC 3 cut(s) 182, 387, 738
Sau3AI GATC 2 cut(s) 771, 981
Sau96I GGNCC 4 cut(s) 497, 588, 653, 869
SbfI CCTGCAGG 1 cut(s) 370
SchI GAGTC 2 cut(s) 749, 920
ScrFI CCNGG 2 cut(s) 269, 574
SdaI CCTGCAGG 1 cut(s) 370
SduI GDGCHC 1 cut(s) 946
SfaNI GCATC 2 cut(s) 180, 559
SfcI CTRYAG 5 cut(s) 83, 345, 366, 609, 899
SfoI GGCGCC 1 cut(s) 45
SinI GGWCC 3 cut(s) 588, 653, 869
SpeI ACTAGT 1 cut(s) 406
Sse8387I CCTGCAGG 1 cut(s) 370
Sse9I AATT 4 cut(s) 399, 732, 947, 1031
SsiI CCGC 1 cut(s) 177
SspDI GGCGCC 1 cut(s) 43
SspI AATATT 1 cut(s) 1097
SspMI CTAG 1 cut(s) 407
StyD4I CCNGG 2 cut(s) 267, 572
StyI CCWWGG 1 cut(s) 518
TaaI ACNGT 1 cut(s) 505
TaiI ACGT 3 cut(s) 55, 810, 908
TaqI TCGA 2 cut(s) 89, 1052
TaqII GACCGA 1 cut(s) 919
TasI AATT 4 cut(s) 399, 732, 947, 1031
TfiI GAWTC 3 cut(s) 61, 300, 1118
Tru1I TTAA 4 cut(s) 74, 129, 395, 1092
Tru9I TTAA 4 cut(s) 74, 129, 395, 1092
TscAI CASTG 8 cut(s) 105, 240, 790, 879, 891, 981, 1071, 1077
TseFI GTSAC 2 cut(s) 283, 688
TseI GCWGC 3 cut(s) 181, 386, 737
Tsp45I GTSAC 2 cut(s) 283, 688
TspDTI ATGAA 1 cut(s) 1061
TspRI CASTG 8 cut(s) 105, 240, 790, 879, 891, 981, 1071, 1077
Tth111I GACNNNGTC 1 cut(s) 929
VneI GTGCAC 1 cut(s) 942
VpaK11BI GGWCC 3 cut(s) 588, 653, 869
XapI RAATTY 1 cut(s) 1031
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 1 cut(s) 407
Zsp2I ATGCAT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.