RchiOBHm_Chr2g0100221

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005515
Physical Location & Seq
Reverse (-)
12201253 .. 12203129
1877 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47487

Sequence Viewer

Length: 1020 bp
ATGGCTGATTTATCATCAAGTGTAGCTAATAAGAGAAGATTGTGTATGGTGCCTGAAAATGCAAGGATTCATATGGCTTCAACTGTCCTTCAGTTCATCTATGCAGGGGTACATATCATTTGCAGAACTATGCTTAATATGAGTGTAAGCATGCTTGTTTTCTCAGTTTACAGGAATGTCACGGCGCTGCTTCTGCTGATTCCATTTGCATACTTTTTGGAGAAGTATGCACAGAATTACAAAAAACTACTACATTTCTTAGTTATCATTATCCATCTGATCTATGCCTCGAAAAGACTTACTAATATATCTTTTCTGTGCAGGAAAGATAGACCACCACTGAATATTTCCCATGTCATGAAGTTTTTTCTCCTAGGACTTCTTGGAATTGAACAAATGCACTTGAATAGAAAATATGGTAAAGCCAAAGTGCTTGGAACTCTTGCTTCTGTTGCTGGAGCCTCAGTCATAACCTTTTACAAGGGGCCAACTATTTATGTTCCTCCAAGTACTTCACCTTTAAGCCAATCCCATATGCTCAACTCATTATTTGGAGATGTCAAGGAGGAGAATTGGACCTTTGGTTACATCTGTGTAATAAGTCATTGCCTATGTTGGTCTACCTGGATTGTGCTACAAGCACCTGTTCTGAAGAAATACCCAGCTCGCCTCTCAGTCAGGTGTGGAGTGGGGGCTTCAGCAATGGCGAATGTAATACAGATATGGGTGATTGACAAGGGAGGACCAGTGTATGTTTCACTGTATCTGCCTTTACAGACGGCAGTTGTAGCTGTAATGGACTCGCTCGTTTTTGGTGAAGAATTCTACTTGGGAAGCATTATTGGAGCAATGTTGACCATAGCAGGGCTGTACCTGGTGGTCTGGGGAAAAAGGCAGGAGAGCAAAATCAAGGTGGAAACTTCCTCAATGCCAGGGAACATTAACAGTCTGATAAACTATTGTTCTGGGGGAGAAGAACCCTCTCTTGTTCAAGCATTGCTCCCTACCTCTGCTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

339

Amino Acids

37.65

Weight (kDa)

9.41

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 228 - 294 4.1e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 49
AccI GTMKAC 1 cut(s) 620
AcsI RAATTY 1 cut(s) 821
AcuI CTGAAG 3 cut(s) 74, 671, 681
AfaI GTAC 3 cut(s) 111, 511, 872
AfiI CCNNNNNNNGG 1 cut(s) 864
AgsI TTSAA 4 cut(s) 81, 392, 406, 992
AjnI CCWGG 3 cut(s) 623, 873, 931
AluBI AGCT 3 cut(s) 26, 665, 791
AluI AGCT 3 cut(s) 26, 665, 791
AoxI GGCC 1 cut(s) 485
ApeKI GCWGC 1 cut(s) 187
ApoI RAATTY 1 cut(s) 821
AspA2I CCTAGG 1 cut(s) 373
AspLEI GCGC 1 cut(s) 187
AspS9I GGNCC 3 cut(s) 485, 576, 743
AsuHPI GGTGA 3 cut(s) 507, 739, 827
AvaII GGWCC 2 cut(s) 576, 743
AvrII CCTAGG 1 cut(s) 373
BanI GGYRCC 1 cut(s) 49
BbvI GCAGC 1 cut(s) 174
BccI CCATC 1 cut(s) 282
BceAI ACGGC 2 cut(s) 198, 795
BciT130I CCWGG 3 cut(s) 625, 875, 933
BfaI CTAG 2 cut(s) 374, 1018
BfoI RGCGCY 1 cut(s) 188
BisI GCNGC 1 cut(s) 188
BlnI CCTAGG 1 cut(s) 373
BlsI GCNGC 1 cut(s) 189
BmcAI AGTACT 1 cut(s) 511
Bme1390I CCNGG 3 cut(s) 625, 875, 933
Bme18I GGWCC 2 cut(s) 576, 743
BmgT120I GGNCC 3 cut(s) 485, 576, 743
BmiI GGNNCC 3 cut(s) 51, 460, 486
BmrFI CCNGG 3 cut(s) 625, 875, 933
BpmI CTGGAG 1 cut(s) 477
BsaJI CCNNGG 2 cut(s) 373, 932
BsaXI ACNNNNNCTCC 4 cut(s) 450, 480, 732, 762
Bsc4I CCNNNNNNNGG 1 cut(s) 864
Bse1I ACTGG 1 cut(s) 746
Bse3DI GCAATG 4 cut(s) 604, 708, 855, 995
BseBI CCWGG 3 cut(s) 625, 875, 933
BseDI CCNNGG 2 cut(s) 373, 932
BseLI CCNNNNNNNGG 1 cut(s) 864
BseMI GCAATG 4 cut(s) 604, 708, 855, 995
BseMII CTCAG 3 cut(s) 177, 477, 687
BseNI ACTGG 1 cut(s) 746
BseRI GAGGAG 1 cut(s) 581
BseXI GCAGC 1 cut(s) 174
BseYI CCCAGC 1 cut(s) 661
BsgI GTGCAG 1 cut(s) 340
BshFI GGCC 1 cut(s) 487
BshNI GGYRCC 1 cut(s) 49
BslI CCNNNNNNNGG 1 cut(s) 864
BsnI GGCC 1 cut(s) 487
Bsp143I GATC 1 cut(s) 279
BspANI GGCC 1 cut(s) 487
BspCNI CTCAG 3 cut(s) 176, 476, 686
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 3 cut(s) 51, 460, 486
BspT107I GGYRCC 1 cut(s) 49
BsrDI GCAATG 4 cut(s) 604, 708, 855, 995
BsrI ACTGG 1 cut(s) 746
BssECI CCNNGG 2 cut(s) 373, 932
BssMI GATC 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 373
Bst2UI CCWGG 3 cut(s) 625, 875, 933
Bst4CI ACNGT 3 cut(s) 85, 762, 947
BstC8I GCNNGC 2 cut(s) 152, 667
BstDEI CTNAG 4 cut(s) 163, 259, 463, 673
BstH2I RGCGCY 1 cut(s) 188
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 1 cut(s) 282
BstMBI GATC 1 cut(s) 279
BstMWI GCNNNNNNNGC 3 cut(s) 193, 452, 788
BstNI CCWGG 3 cut(s) 625, 875, 933
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 3 cut(s) 623, 873, 931
BstV1I GCAGC 1 cut(s) 174
BsuRI GGCC 1 cut(s) 487
BtsIMutI CAGTG 3 cut(s) 338, 753, 758
Cac8I GCNNGC 2 cut(s) 152, 667
CciI TCATGA 1 cut(s) 357
CfoI GCGC 1 cut(s) 187
Cfr13I GGNCC 3 cut(s) 485, 576, 743
CsiI ACCWGGT 1 cut(s) 873
Csp6I GTAC 3 cut(s) 110, 510, 871
CviAII CATG 3 cut(s) 151, 353, 358
CviQI GTAC 3 cut(s) 110, 510, 871
DdeI CTNAG 4 cut(s) 163, 259, 463, 673
DpnI GATC 1 cut(s) 281
DpnII GATC 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 373
Eco47I GGWCC 2 cut(s) 576, 743
Eco57I CTGAAG 3 cut(s) 74, 671, 681
EcoRI GAATTC 1 cut(s) 821
EcoRII CCWGG 3 cut(s) 623, 873, 931
EcoT14I CCWWGG 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 373
FaeI CATG 3 cut(s) 154, 356, 361
FatI CATG 3 cut(s) 150, 352, 357
FauNDI CATATG 2 cut(s) 72, 534
FblI GTMKAC 1 cut(s) 620
Fnu4HI GCNGC 1 cut(s) 188
Fsp4HI GCNGC 1 cut(s) 188
FspBI CTAG 2 cut(s) 374, 1018
GlaI GCGC 1 cut(s) 186
GluI GCNGC 1 cut(s) 188
GsaI CCCAGC 1 cut(s) 665
GsuI CTGGAG 1 cut(s) 477
HaeII RGCGCY 1 cut(s) 188
HaeIII GGCC 1 cut(s) 487
HhaI GCGC 1 cut(s) 187
Hin1II CATG 3 cut(s) 154, 356, 361
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HincII GTYRAC 1 cut(s) 855
HindII GTYRAC 1 cut(s) 855
HinfI GANTC 3 cut(s) 67, 199, 800
HphI GGTGA 3 cut(s) 507, 739, 827
Hpy166II GTNNAC 3 cut(s) 169, 621, 855
Hpy188I TCNGA 3 cut(s) 279, 651, 951
Hpy188III TCNNGA 1 cut(s) 358
Hpy8I GTNNAC 3 cut(s) 169, 621, 855
HpyAV CCTTC 1 cut(s) 98
HpyCH4III ACNGT 3 cut(s) 85, 762, 947
HpyCH4V TGCA 7 cut(s) 62, 104, 123, 209, 230, 321, 400
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 452, 788
HpyF3I CTNAG 4 cut(s) 163, 259, 463, 673
Hsp92II CATG 3 cut(s) 154, 356, 361
HspAI GCGC 1 cut(s) 185
Kzo9I GATC 1 cut(s) 279
LmnI GCTCC 3 cut(s) 458, 845, 1005
Lsp1109I GCAGC 1 cut(s) 174
MabI ACCWGGT 1 cut(s) 873
MaeI CTAG 2 cut(s) 374, 1018
MaeIII GTNAC 2 cut(s) 178, 584
MalI GATC 1 cut(s) 281
MboI GATC 1 cut(s) 279
MboII GAAGA 4 cut(s) 48, 664, 830, 986
MluCI AATT 4 cut(s) 235, 387, 571, 821
MlyI GAGTC 1 cut(s) 794
MnlI CCTC 9 cut(s) 298, 472, 513, 559, 680, 734, 934, 991, 1018
MseI TTAA 3 cut(s) 135, 521, 942
MspR9I CCNGG 3 cut(s) 625, 875, 933
MvaI CCWGG 3 cut(s) 625, 875, 933
MwoI GCNNNNNNNGC 3 cut(s) 193, 452, 788
NdeI CATATG 2 cut(s) 72, 534
NdeII GATC 1 cut(s) 279
NlaIII CATG 3 cut(s) 154, 356, 361
NlaIV GGNNCC 3 cut(s) 51, 460, 486
NmuCI GTSAC 1 cut(s) 178
NspI RCATGY 1 cut(s) 154
PaeI GCATGC 1 cut(s) 154
PagI TCATGA 1 cut(s) 357
PfeI GAWTC 2 cut(s) 67, 199
PkrI GCNGC 1 cut(s) 189
PleI GAGTC 1 cut(s) 794
PpsI GAGTC 1 cut(s) 794
Psp6I CCWGG 3 cut(s) 623, 873, 931
PspFI CCCAGC 1 cut(s) 661
PspGI CCWGG 3 cut(s) 623, 873, 931
PspN4I GGNNCC 3 cut(s) 51, 460, 486
PspPI GGNCC 3 cut(s) 485, 576, 743
RsaI GTAC 3 cut(s) 111, 511, 872
RsaNI GTAC 3 cut(s) 110, 510, 871
SaqAI TTAA 3 cut(s) 135, 521, 942
SatI GCNGC 1 cut(s) 188
Sau3AI GATC 1 cut(s) 279
Sau96I GGNCC 3 cut(s) 485, 576, 743
ScaI AGTACT 1 cut(s) 511
SchI GAGTC 1 cut(s) 794
ScrFI CCNGG 3 cut(s) 625, 875, 933
SexAI ACCWGGT 1 cut(s) 873
SinI GGWCC 2 cut(s) 576, 743
SphI GCATGC 1 cut(s) 154
Sse9I AATT 4 cut(s) 235, 387, 571, 821
SspI AATATT 1 cut(s) 346
SspMI CTAG 2 cut(s) 374, 1018
StyD4I CCNGG 3 cut(s) 623, 873, 931
StyI CCWWGG 1 cut(s) 373
TaaI ACNGT 3 cut(s) 85, 762, 947
TaqI TCGA 1 cut(s) 290
TasI AATT 4 cut(s) 235, 387, 571, 821
TatI WGTACW 1 cut(s) 509
TfiI GAWTC 2 cut(s) 67, 199
Tru1I TTAA 3 cut(s) 135, 521, 942
Tru9I TTAA 3 cut(s) 135, 521, 942
TscAI CASTG 3 cut(s) 345, 753, 765
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 1 cut(s) 187
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 3 cut(s) 59, 85, 374
TspRI CASTG 3 cut(s) 345, 753, 765
VpaK11BI GGWCC 2 cut(s) 576, 743
XapI RAATTY 1 cut(s) 821
XceI RCATGY 1 cut(s) 154
XmaJI CCTAGG 1 cut(s) 373
XmiI GTMKAC 1 cut(s) 620
XspI CTAG 2 cut(s) 374, 1018
ZrmI AGTACT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.