RchiOBHm_Chr2g0100241

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005515
Physical Location & Seq
Reverse (-)
12224325 .. 12226531
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47489

Sequence Viewer

Length: 1155 bp
ATGGCTGATGAGGCTGGTTCATCATCAAGTACTAGTAGAGCTCATGGCAAGAGACTATGGTGTATGATGACTGAACGTGCCCGGATTCACATGGCATTGACTGCCCTGCAGTTCAGTTATGCAGGGCAACATGTCATCCTCAGAACTGTGCTTAACATGGGTGTAAGCAAGCTTGTGTTCCCAGTTTACAGGAACATCATCGCGCTGCTTCTGCTCGGTCCCTTTGCATACTTTCTAGAGAAGAAAGATAGACCACCACTAAATTTTGGCCTTGTCATGCAGTTTTTCCTCATGGGACTTCTTGGGATCACATGCAATCAAGGATTGTACCTCTTGGGTTTGGAAAACTCTTCGCCAACATTTGCTTCTGCAATAGAGAATGCAGTCCCTGCAATTACATTTTTCATGGCTGCACTATTCAGAATTGAACAAGTTCACTTGAATAGAAAAGATGGCAAAGCCAAGGTGCTTGGAACTCTGGCTTCTGTTGCTGGAGCCTCAGTCATAACCATTTACAAAGGGCCAACAATTTACGGACCCCCAAGTGATTCACAATATTTAAACCAATCTTATTCTCTGTTCTCATCATTTGGAGATGCCAAGGAGAAGAATTGGACCTTGGGATGCATATGTCTAATTGGTCATTGCCTGTGTTGGTCTAGCTGGATTGTGCTACAAGCACCGGTTTTGAAGAAATACCCAGCTCGCCTTTCAGTCACCTCATTTACTTGCTTCTTTGGCATTCTGCAATGTTCTGCAATCGCGGGGATTGTAGAGAGAGATTCCCAAGCCTGGCAGGTCATCCATTCTGGTGGTGAAGTTGTTGCTATTCTCTACGCGGGAGTGGTGGCTTCAGCAATGGCATTTGCATTACAGATATGGGTGATTGAACGAGGAGGCCCAGTGTTCGTTTCAGTGTACCTGCCTCTACAGACATTACTGGTAGCTCTAATGGCCTCAGTCATTTTAGGTGAACAATTCTACTTGGGAGGCATTATTGGAGCAGTATTGATCGTAGCCGGACTCTACCTGGTGGTCTGGGGGAAAAATGAAGAGAGCAAGTTTGGCAATGAAAATGGTGAAATTCACCGATCAATGCCTGCAGATGAACAATTCTCTCTCTTTCAGTCATTTCTCGGTTCCTCTTCTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

384

Amino Acids

41.86

Weight (kDa)

8.7

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 37 - 170 3.7e-13 EamA-like transporter family
EamA PF00892 207 - 346 8.3e-17 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 787, 930
AccII CGCG 3 cut(s) 203, 764, 839
AciI CCGC 2 cut(s) 764, 839
AclWI GGATC 1 cut(s) 314
AcsI RAATTY 2 cut(s) 262, 1083
AcuI CTGAAG 1 cut(s) 837
AfaI GTAC 3 cut(s) 31, 329, 920
AfiI CCNNNNNNNGG 1 cut(s) 792
AflIII ACRYGT 1 cut(s) 130
AgeI ACCGGT 1 cut(s) 682
AgsI TTSAA 4 cut(s) 428, 442, 691, 890
AhlI ACTAGT 1 cut(s) 32
AjnI CCWGG 2 cut(s) 791, 1029
AjuI GAANNNNNNNTTGG 4 cut(s) 349, 381, 602, 634
AluBI AGCT 5 cut(s) 41, 172, 663, 704, 947
AluI AGCT 5 cut(s) 41, 172, 663, 704, 947
Alw21I GWGCWC 1 cut(s) 43
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 1 cut(s) 314
AlwNI CAGNNNCTG 1 cut(s) 389
AoxI GGCC 4 cut(s) 268, 521, 898, 954
ApeKI GCWGC 2 cut(s) 205, 410
ApoI RAATTY 2 cut(s) 262, 1083
AsiGI ACCGGT 1 cut(s) 682
Asp700I GAANNNNTTC 1 cut(s) 432
AspLEI GCGC 1 cut(s) 205
AspS9I GGNCC 5 cut(s) 218, 521, 536, 615, 899
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 6 cut(s) 709, 827, 895, 983, 1079, 1091
AvaII GGWCC 3 cut(s) 218, 536, 615
BaeGI GKGCMC 1 cut(s) 82
BanII GRGCYC 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 43
BbvI GCAGC 2 cut(s) 192, 397
BccI CCATC 1 cut(s) 446
BciT130I CCWGG 2 cut(s) 793, 1031
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 1 cut(s) 46
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 3 cut(s) 33, 236, 660
BfmI CTRYAG 3 cut(s) 107, 929, 1101
BfuAI ACCTGC 2 cut(s) 787, 930
BisI GCNGC 2 cut(s) 206, 411
BlsI GCNGC 2 cut(s) 207, 412
BmcAI AGTACT 1 cut(s) 31
Bme1390I CCNGG 3 cut(s) 82, 793, 1031
Bme18I GGWCC 3 cut(s) 218, 536, 615
BmgT120I GGNCC 5 cut(s) 218, 521, 536, 615, 899
BmiI GGNNCC 4 cut(s) 220, 496, 538, 1141
BmrFI CCNGG 3 cut(s) 82, 793, 1031
BmrI ACTGGG 2 cut(s) 176, 896
BmsI GCATC 2 cut(s) 586, 614
BmuI ACTGGG 2 cut(s) 176, 896
BpmI CTGGAG 1 cut(s) 513
BpuMI CCSGG 1 cut(s) 82
BsaJI CCNNGG 3 cut(s) 462, 600, 618
BsaWI WCCGGW 1 cut(s) 682
BsaXI ACNNNNNCTCC 4 cut(s) 486, 516, 888, 918
Bsc4I CCNNNNNNNGG 1 cut(s) 792
Bse118I RCCGGY 1 cut(s) 682
Bse1I ACTGG 3 cut(s) 182, 902, 945
Bse3DI GCAATG 4 cut(s) 643, 755, 864, 1075
BseBI CCWGG 2 cut(s) 793, 1031
BseDI CCNNGG 3 cut(s) 462, 600, 618
BseGI GGATG 3 cut(s) 135, 629, 801
BseLI CCNNNNNNNGG 1 cut(s) 792
BseMI GCAATG 4 cut(s) 643, 755, 864, 1075
BseMII CTCAG 3 cut(s) 154, 513, 972
BseNI ACTGG 3 cut(s) 182, 902, 945
BseRI GAGGAG 1 cut(s) 909
BseSI GKGCMC 1 cut(s) 82
BseXI GCAGC 2 cut(s) 192, 397
BseYI CCCAGC 1 cut(s) 700
BsgI GTGCAG 1 cut(s) 396
Bsh1236I CGCG 3 cut(s) 203, 764, 839
BshFI GGCC 4 cut(s) 270, 523, 900, 956
BshTI ACCGGT 1 cut(s) 682
BsiHKAI GWGCWC 1 cut(s) 43
BsiSI CCGG 3 cut(s) 82, 683, 1020
BslFI GGGAC 3 cut(s) 204, 309, 371
BslI CCNNNNNNNGG 1 cut(s) 792
BsmAI GTCTC 1 cut(s) 46
BsmFI GGGAC 3 cut(s) 204, 309, 371
BsmI GAATGC 2 cut(s) 385, 741
BsnI GGCC 4 cut(s) 270, 523, 900, 956
Bsp1286I GDGCHC 2 cut(s) 43, 82
Bsp143I GATC 3 cut(s) 306, 1011, 1091
BspACI CCGC 2 cut(s) 764, 839
BspANI GGCC 4 cut(s) 270, 523, 900, 956
BspCNI CTCAG 3 cut(s) 153, 512, 971
BspFNI CGCG 3 cut(s) 203, 764, 839
BspLI GGNNCC 4 cut(s) 220, 496, 538, 1141
BspMAI CTGCAG 2 cut(s) 111, 1105
BspMI ACCTGC 2 cut(s) 787, 930
BspPI GGATC 1 cut(s) 314
BsrDI GCAATG 4 cut(s) 643, 755, 864, 1075
BsrFI RCCGGY 1 cut(s) 682
BsrI ACTGG 3 cut(s) 182, 902, 945
BssAI RCCGGY 1 cut(s) 682
BssECI CCNNGG 3 cut(s) 462, 600, 618
BssMI GATC 3 cut(s) 306, 1011, 1091
BssT1I CCWWGG 3 cut(s) 462, 600, 618
Bst2UI CCWGG 2 cut(s) 793, 1031
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 3 cut(s) 355, 1047, 1150
BstAPI GCANNNNNTGC 2 cut(s) 101, 389
BstC8I GCNNGC 3 cut(s) 170, 706, 1101
BstDEI CTNAG 4 cut(s) 140, 499, 958, 1149
BstF5I GGATG 3 cut(s) 135, 629, 801
BstFNI CGCG 3 cut(s) 203, 764, 839
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 3 cut(s) 309, 1014, 1094
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 3 cut(s) 306, 1011, 1091
BstMWI GCNNNNNNNGC 8 cut(s) 11, 101, 211, 389, 488, 738, 953, 1065
BstNI CCWGG 2 cut(s) 793, 1031
BstNSI RCATGY 2 cut(s) 134, 315
BstSCI CCNGG 3 cut(s) 80, 791, 1029
BstSFI CTRYAG 3 cut(s) 107, 929, 1101
BstSLI GKGCMC 1 cut(s) 82
BstUI CGCG 3 cut(s) 203, 764, 839
BstV1I GCAGC 2 cut(s) 192, 397
BstXI CCANNNNNNTGG 1 cut(s) 812
BsuRI GGCC 4 cut(s) 270, 523, 900, 956
BtgZI GCGATG 1 cut(s) 184
BtsCI GGATG 3 cut(s) 135, 629, 801
BtsIMutI CAGTG 2 cut(s) 909, 921
BveI ACCTGC 2 cut(s) 787, 930
Cac8I GCNNGC 3 cut(s) 170, 706, 1101
CaiI CAGNNNCTG 1 cut(s) 389
CfoI GCGC 1 cut(s) 205
Cfr10I RCCGGY 1 cut(s) 682
Cfr13I GGNCC 5 cut(s) 218, 521, 536, 615, 899
CsiI ACCWGGT 1 cut(s) 1029
Csp6I GTAC 3 cut(s) 30, 328, 919
CspAI ACCGGT 1 cut(s) 682
CviAII CATG 8 cut(s) 44, 91, 131, 157, 277, 292, 312, 406
CviQI GTAC 3 cut(s) 30, 328, 919
DdeI CTNAG 4 cut(s) 140, 499, 958, 1149
DpnI GATC 3 cut(s) 308, 1013, 1093
DpnII GATC 3 cut(s) 306, 1011, 1091
DraI TTTAAA 1 cut(s) 561
Eam1104I CTCTTC 3 cut(s) 355, 1047, 1150
EarI CTCTTC 3 cut(s) 355, 1047, 1150
Ecl136II GAGCTC 1 cut(s) 41
Eco130I CCWWGG 3 cut(s) 462, 600, 618
Eco24I GRGCYC 1 cut(s) 43
Eco47I GGWCC 3 cut(s) 218, 536, 615
Eco53kI GAGCTC 1 cut(s) 41
Eco57I CTGAAG 1 cut(s) 837
EcoICRI GAGCTC 1 cut(s) 41
EcoRII CCWGG 2 cut(s) 791, 1029
EcoT14I CCWWGG 3 cut(s) 462, 600, 618
EcoT22I ATGCAT 1 cut(s) 629
EcoT38I GRGCYC 1 cut(s) 43
ErhI CCWWGG 3 cut(s) 462, 600, 618
FaeI CATG 8 cut(s) 47, 94, 134, 160, 280, 295, 315, 409
FaqI GGGAC 3 cut(s) 204, 309, 371
FatI CATG 8 cut(s) 43, 90, 130, 156, 276, 291, 311, 405
FauI CCCGC 2 cut(s) 757, 832
FauNDI CATATG 1 cut(s) 629
Fnu4HI GCNGC 2 cut(s) 206, 411
FokI GGATG 3 cut(s) 122, 636, 788
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 2 cut(s) 206, 411
FspBI CTAG 3 cut(s) 33, 236, 660
GlaI GCGC 1 cut(s) 204
GluI GCNGC 2 cut(s) 206, 411
GsaI CCCAGC 1 cut(s) 704
GsuI CTGGAG 1 cut(s) 513
HaeIII GGCC 4 cut(s) 270, 523, 900, 956
HapII CCGG 3 cut(s) 82, 683, 1020
HhaI GCGC 1 cut(s) 205
Hin1II CATG 8 cut(s) 47, 94, 134, 160, 280, 295, 315, 409
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 4 cut(s) 85, 548, 782, 1023
HpaII CCGG 3 cut(s) 82, 683, 1020
HphI GGTGA 6 cut(s) 709, 827, 895, 983, 1079, 1091
Hpy166II GTNNAC 4 cut(s) 187, 436, 919, 974
Hpy188I TCNGA 2 cut(s) 143, 422
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 4 cut(s) 187, 436, 919, 974
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 101, 211, 389, 488, 738, 953, 1065
HpyF3I CTNAG 4 cut(s) 140, 499, 958, 1149
HpySE526I ACGT 1 cut(s) 76
Hsp92II CATG 8 cut(s) 47, 94, 134, 160, 280, 295, 315, 409
HspAI GCGC 1 cut(s) 203
Kzo9I GATC 3 cut(s) 306, 1011, 1091
LmnI GCTCC 2 cut(s) 494, 1001
Lsp1109I GCAGC 2 cut(s) 192, 397
LweI GCATC 2 cut(s) 586, 614
MabI ACCWGGT 1 cut(s) 1029
MaeI CTAG 3 cut(s) 33, 236, 660
MaeII ACGT 1 cut(s) 76
MaeIII GTNAC 1 cut(s) 715
MalI GATC 3 cut(s) 308, 1013, 1093
MboI GATC 3 cut(s) 306, 1011, 1091
MboII GAAGA 6 cut(s) 253, 342, 619, 703, 1064, 1137
MhlI GDGCHC 2 cut(s) 43, 82
MluCI AATT 9 cut(s) 262, 393, 423, 528, 610, 636, 977, 1083, 1112
MlyI GAGTC 1 cut(s) 1017
Mph1103I ATGCAT 1 cut(s) 629
MroXI GAANNNNTTC 1 cut(s) 432
MseI TTAA 2 cut(s) 153, 560
MslI CAYNNNNRTG 1 cut(s) 810
MspI CCGG 3 cut(s) 82, 683, 1020
MspR9I CCNGG 3 cut(s) 82, 793, 1031
Mva1269I GAATGC 2 cut(s) 385, 741
MvaI CCWGG 2 cut(s) 793, 1031
MvnI CGCG 3 cut(s) 203, 764, 839
MwoI GCNNNNNNNGC 8 cut(s) 11, 101, 211, 389, 488, 738, 953, 1065
NciI CCSGG 1 cut(s) 82
NdeI CATATG 1 cut(s) 629
NdeII GATC 3 cut(s) 306, 1011, 1091
NlaIII CATG 8 cut(s) 47, 94, 134, 160, 280, 295, 315, 409
NlaIV GGNNCC 4 cut(s) 220, 496, 538, 1141
NmuCI GTSAC 1 cut(s) 715
NsiI ATGCAT 1 cut(s) 629
NspI RCATGY 2 cut(s) 134, 315
PciI ACATGT 1 cut(s) 130
PctI GAATGC 2 cut(s) 385, 741
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 3 cut(s) 85, 548, 782
PinAI ACCGGT 1 cut(s) 682
PkrI GCNGC 2 cut(s) 207, 412
PleI GAGTC 1 cut(s) 1017
PpsI GAGTC 1 cut(s) 1017
PscI ACATGT 1 cut(s) 130
Psp124BI GAGCTC 1 cut(s) 43
Psp6I CCWGG 2 cut(s) 791, 1029
PspFI CCCAGC 1 cut(s) 700
PspGI CCWGG 2 cut(s) 791, 1029
PspN4I GGNNCC 4 cut(s) 220, 496, 538, 1141
PspPI GGNCC 5 cut(s) 218, 521, 536, 615, 899
PstI CTGCAG 2 cut(s) 111, 1105
PstNI CAGNNNCTG 1 cut(s) 389
RsaI GTAC 3 cut(s) 31, 329, 920
RsaNI GTAC 3 cut(s) 30, 328, 919
RseI CAYNNNNRTG 1 cut(s) 810
SacI GAGCTC 1 cut(s) 43
SaqAI TTAA 2 cut(s) 153, 560
SatI GCNGC 2 cut(s) 206, 411
Sau3AI GATC 3 cut(s) 306, 1011, 1091
Sau96I GGNCC 5 cut(s) 218, 521, 536, 615, 899
ScaI AGTACT 1 cut(s) 31
SchI GAGTC 1 cut(s) 1017
ScrFI CCNGG 3 cut(s) 82, 793, 1031
SduI GDGCHC 2 cut(s) 43, 82
SexAI ACCWGGT 1 cut(s) 1029
SfaNI GCATC 2 cut(s) 586, 614
SfcI CTRYAG 3 cut(s) 107, 929, 1101
SinI GGWCC 3 cut(s) 218, 536, 615
SmiMI CAYNNNNRTG 1 cut(s) 810
SpeI ACTAGT 1 cut(s) 32
Sse9I AATT 9 cut(s) 262, 393, 423, 528, 610, 636, 977, 1083, 1112
SsiI CCGC 2 cut(s) 764, 839
SspI AATATT 1 cut(s) 557
SspMI CTAG 3 cut(s) 33, 236, 660
SstI GAGCTC 1 cut(s) 43
StyD4I CCNGG 3 cut(s) 80, 791, 1029
StyI CCWWGG 3 cut(s) 462, 600, 618
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 1 cut(s) 79
TaqII GACCGA 1 cut(s) 206
TasI AATT 9 cut(s) 262, 393, 423, 528, 610, 636, 977, 1083, 1112
TatI WGTACW 1 cut(s) 29
TfiI GAWTC 3 cut(s) 85, 548, 782
Tru1I TTAA 2 cut(s) 153, 560
Tru9I TTAA 2 cut(s) 153, 560
TscAI CASTG 2 cut(s) 909, 921
TseFI GTSAC 1 cut(s) 715
TseI GCWGC 2 cut(s) 205, 410
Tsp45I GTSAC 1 cut(s) 715
TspDTI ATGAA 5 cut(s) 9, 394, 1065, 1086, 1122
TspGWI ACGGA 1 cut(s) 549
TspRI CASTG 2 cut(s) 909, 921
VpaK11BI GGWCC 3 cut(s) 218, 536, 615
XapI RAATTY 2 cut(s) 262, 1083
XbaI TCTAGA 1 cut(s) 235
XceI RCATGY 2 cut(s) 134, 315
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 3 cut(s) 33, 236, 660
ZrmI AGTACT 1 cut(s) 31
Zsp2I ATGCAT 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.